LOCUS       DAYYKC010000001       101832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000001 DAYYKC010000000
VERSION     DAYYKC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.001"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2200)
                     /locus_tag="ACHBBW_000001"
     CDS             complement(<1..2200)
                     /locus_tag="ACHBBW_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151721.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYK"
     gene            complement(2323..3873)
                     /locus_tag="ACHBBW_000002"
     CDS             complement(2323..3873)
                     /locus_tag="ACHBBW_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9151722.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3875..4291)
                     /locus_tag="ACHBBW_000003"
     CDS             complement(3875..4291)
                     /locus_tag="ACHBBW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151723.1"
                     /translation="MNNTIPYGYVIKNGEINVDEQQAQQIREFFNIYLEGVGLQEAGK
                     RSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVP
                     DTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            complement(4288..5841)
                     /locus_tag="ACHBBW_000004"
     CDS             complement(4288..5841)
                     /locus_tag="ACHBBW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9151724.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5902..6075)
                     /locus_tag="ACHBBW_000005"
     CDS             complement(5902..6075)
                     /locus_tag="ACHBBW_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM9151725.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6370..6675
                     /locus_tag="ACHBBW_000006"
     CDS             6370..6675
                     /locus_tag="ACHBBW_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151726.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6736..7773)
                     /locus_tag="ACHBBW_000007"
     CDS             complement(6736..7773)
                     /locus_tag="ACHBBW_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9151727.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7889..8797)
                     /locus_tag="ACHBBW_000008"
     CDS             complement(7889..8797)
                     /locus_tag="ACHBBW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9151728.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8787..9206)
                     /locus_tag="ACHBBW_000009"
     CDS             complement(8787..9206)
                     /locus_tag="ACHBBW_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM9151729.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9207..9614)
                     /locus_tag="ACHBBW_000010"
     CDS             complement(9207..9614)
                     /locus_tag="ACHBBW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM9151730.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9601..9768)
                     /locus_tag="ACHBBW_000011"
     CDS             complement(9601..9768)
                     /locus_tag="ACHBBW_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151731.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9778..13668)
                     /locus_tag="ACHBBW_000012"
     CDS             complement(9778..13668)
                     /locus_tag="ACHBBW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9151732.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13665..14357)
                     /locus_tag="ACHBBW_000013"
     CDS             complement(13665..14357)
                     /locus_tag="ACHBBW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9151733.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14434..14835)
                     /locus_tag="ACHBBW_000014"
     CDS             complement(14434..14835)
                     /locus_tag="ACHBBW_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151734.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14906..16966)
                     /locus_tag="ACHBBW_000015"
     CDS             complement(14906..16966)
                     /locus_tag="ACHBBW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9151735.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            complement(17674..18054)
                     /locus_tag="ACHBBW_000016"
     CDS             complement(17674..18054)
                     /locus_tag="ACHBBW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151736.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(18064..18654)
                     /locus_tag="ACHBBW_000017"
     CDS             complement(18064..18654)
                     /locus_tag="ACHBBW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9151737.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18657..18998)
                     /locus_tag="ACHBBW_000018"
     CDS             complement(18657..18998)
                     /locus_tag="ACHBBW_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151738.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(18995..19426)
                     /locus_tag="ACHBBW_000019"
     CDS             complement(18995..19426)
                     /locus_tag="ACHBBW_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9151739.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19419..19757)
                     /locus_tag="ACHBBW_000020"
     CDS             complement(19419..19757)
                     /locus_tag="ACHBBW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9151740.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19754..20065)
                     /locus_tag="ACHBBW_000021"
     CDS             complement(19754..20065)
                     /locus_tag="ACHBBW_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9151741.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(20076..21287)
                     /locus_tag="ACHBBW_000022"
     CDS             complement(20076..21287)
                     /locus_tag="ACHBBW_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9151742.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21291..22046)
                     /locus_tag="ACHBBW_000023"
     CDS             complement(21291..22046)
                     /locus_tag="ACHBBW_000023"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9151743.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21946..23274)
                     /locus_tag="ACHBBW_000024"
     CDS             complement(21946..23274)
                     /locus_tag="ACHBBW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9151744.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23340..24074)
                     /locus_tag="ACHBBW_000025"
     CDS             complement(23340..24074)
                     /locus_tag="ACHBBW_000025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151745.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24130..25695)
                     /locus_tag="ACHBBW_000026"
     CDS             complement(24130..25695)
                     /locus_tag="ACHBBW_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9151746.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25778..25921)
                     /locus_tag="ACHBBW_000027"
     CDS             complement(25778..25921)
                     /locus_tag="ACHBBW_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151747.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25918..26094)
                     /locus_tag="ACHBBW_000028"
     CDS             complement(25918..26094)
                     /locus_tag="ACHBBW_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM9151748.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(26078..26272)
                     /locus_tag="ACHBBW_000029"
     CDS             complement(26078..26272)
                     /locus_tag="ACHBBW_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151749.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26274..26651)
                     /locus_tag="ACHBBW_000030"
     CDS             complement(26274..26651)
                     /locus_tag="ACHBBW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM9151750.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26725..27960)
                     /locus_tag="ACHBBW_000031"
     CDS             complement(26725..27960)
                     /locus_tag="ACHBBW_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM9151751.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27960..28742)
                     /locus_tag="ACHBBW_000032"
     CDS             complement(27960..28742)
                     /locus_tag="ACHBBW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM9151752.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28757..29329)
                     /locus_tag="ACHBBW_000033"
     CDS             complement(28757..29329)
                     /locus_tag="ACHBBW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9151753.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29463..29828)
                     /locus_tag="ACHBBW_000034"
     CDS             complement(29463..29828)
                     /locus_tag="ACHBBW_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9151754.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(29996..30424)
                     /locus_tag="ACHBBW_000035"
     CDS             complement(29996..30424)
                     /locus_tag="ACHBBW_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM9151755.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30421..31773)
                     /locus_tag="ACHBBW_000036"
     CDS             complement(30421..31773)
                     /locus_tag="ACHBBW_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM9151756.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31773..32057)
                     /locus_tag="ACHBBW_000037"
     CDS             complement(31773..32057)
                     /locus_tag="ACHBBW_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM9151757.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(32054..32263)
                     /locus_tag="ACHBBW_000038"
     CDS             complement(32054..32263)
                     /locus_tag="ACHBBW_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151758.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32401..34644)
                     /locus_tag="ACHBBW_000039"
     CDS             complement(32401..34644)
                     /locus_tag="ACHBBW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM9151759.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34637..34981)
                     /locus_tag="ACHBBW_000040"
     CDS             complement(34637..34981)
                     /locus_tag="ACHBBW_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM9151760.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34974..35744)
                     /locus_tag="ACHBBW_000041"
     CDS             complement(34974..35744)
                     /locus_tag="ACHBBW_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9151761.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35955..37891)
                     /locus_tag="ACHBBW_000042"
                     /pseudo
     CDS             complement(35955..37891)
                     /locus_tag="ACHBBW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
     gene            complement(37952..38494)
                     /locus_tag="ACHBBW_000043"
     CDS             complement(37952..38494)
                     /locus_tag="ACHBBW_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM9151762.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38496..39638)
                     /locus_tag="ACHBBW_000044"
     CDS             complement(38496..39638)
                     /locus_tag="ACHBBW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM9151763.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39639..39953)
                     /locus_tag="ACHBBW_000045"
     CDS             complement(39639..39953)
                     /locus_tag="ACHBBW_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM9151764.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39958..40164)
                     /locus_tag="ACHBBW_000046"
     CDS             complement(39958..40164)
                     /locus_tag="ACHBBW_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151765.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40261..40878)
                     /locus_tag="ACHBBW_000047"
     CDS             complement(40261..40878)
                     /locus_tag="ACHBBW_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151766.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41273..42223
                     /locus_tag="ACHBBW_000048"
     CDS             41273..42223
                     /locus_tag="ACHBBW_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM9151767.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42216..42662
                     /locus_tag="ACHBBW_000049"
     CDS             42216..42662
                     /locus_tag="ACHBBW_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM9151768.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42665..44497
                     /locus_tag="ACHBBW_000050"
     CDS             42665..44497
                     /locus_tag="ACHBBW_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9151769.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44513..44716
                     /locus_tag="ACHBBW_000051"
     CDS             44513..44716
                     /locus_tag="ACHBBW_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9151770.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44767..45903)
                     /locus_tag="ACHBBW_000052"
     CDS             complement(44767..45903)
                     /locus_tag="ACHBBW_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9151771.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45893..47848)
                     /locus_tag="ACHBBW_000053"
     CDS             complement(45893..47848)
                     /locus_tag="ACHBBW_000053"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9151772.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47841..50558)
                     /locus_tag="ACHBBW_000054"
     CDS             complement(47841..50558)
                     /locus_tag="ACHBBW_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM9151773.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(50559..50780)
                     /locus_tag="ACHBBW_000055"
     CDS             complement(50559..50780)
                     /locus_tag="ACHBBW_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9151774.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(50929..51765)
                     /locus_tag="ACHBBW_000056"
     CDS             complement(50929..51765)
                     /locus_tag="ACHBBW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9151775.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(51974..53350)
                     /gene="rlmD"
                     /locus_tag="ACHBBW_000057"
     CDS             complement(51974..53350)
                     /gene="rlmD"
                     /locus_tag="ACHBBW_000057"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9151776.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(53461..54516)
                     /locus_tag="ACHBBW_000058"
     CDS             complement(53461..54516)
                     /locus_tag="ACHBBW_000058"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9151777.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(54526..55956)
                     /gene="gatB"
                     /locus_tag="ACHBBW_000059"
     CDS             complement(54526..55956)
                     /gene="gatB"
                     /locus_tag="ACHBBW_000059"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9151778.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(55953..57425)
                     /gene="gatA"
                     /locus_tag="ACHBBW_000060"
     CDS             complement(55953..57425)
                     /gene="gatA"
                     /locus_tag="ACHBBW_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9151779.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(57425..57730)
                     /gene="gatC"
                     /locus_tag="ACHBBW_000061"
     CDS             complement(57425..57730)
                     /gene="gatC"
                     /locus_tag="ACHBBW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9151780.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(57836..59872)
                     /gene="ligA"
                     /locus_tag="ACHBBW_000062"
     CDS             complement(57836..59872)
                     /gene="ligA"
                     /locus_tag="ACHBBW_000062"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9151781.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(59889..62123)
                     /gene="pcrA"
                     /locus_tag="ACHBBW_000063"
     CDS             complement(59889..62123)
                     /gene="pcrA"
                     /locus_tag="ACHBBW_000063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9151782.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            62278..63837
                     /locus_tag="ACHBBW_000064"
     CDS             62278..63837
                     /locus_tag="ACHBBW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9151783.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(63948..65879)
                     /locus_tag="ACHBBW_000065"
     CDS             complement(63948..65879)
                     /locus_tag="ACHBBW_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9151784.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(65897..66811)
                     /gene="pfkB"
                     /locus_tag="ACHBBW_000066"
     CDS             complement(65897..66811)
                     /gene="pfkB"
                     /locus_tag="ACHBBW_000066"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9151785.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(66808..67560)
                     /locus_tag="ACHBBW_000067"
     CDS             complement(66808..67560)
                     /locus_tag="ACHBBW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9151786.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(67719..67928)
                     /locus_tag="ACHBBW_000068"
     CDS             complement(67719..67928)
                     /locus_tag="ACHBBW_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151787.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(67939..69267)
                     /locus_tag="ACHBBW_000069"
     CDS             complement(67939..69267)
                     /locus_tag="ACHBBW_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151788.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            69403..69558
                     /locus_tag="ACHBBW_000070"
     CDS             69403..69558
                     /locus_tag="ACHBBW_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9151789.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(69632..70219)
                     /gene="yihA"
                     /locus_tag="ACHBBW_000071"
     CDS             complement(69632..70219)
                     /gene="yihA"
                     /locus_tag="ACHBBW_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9151790.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(70302..71552)
                     /gene="clpX"
                     /locus_tag="ACHBBW_000072"
     CDS             complement(70302..71552)
                     /gene="clpX"
                     /locus_tag="ACHBBW_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9151791.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(71799..73088)
                     /gene="tig"
                     /locus_tag="ACHBBW_000073"
     CDS             complement(71799..73088)
                     /gene="tig"
                     /locus_tag="ACHBBW_000073"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9151792.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(73242..74174)
                     /locus_tag="ACHBBW_000074"
     CDS             complement(73242..74174)
                     /locus_tag="ACHBBW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151793.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(74331..74990)
                     /locus_tag="ACHBBW_000075"
     CDS             complement(74331..74990)
                     /locus_tag="ACHBBW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9151794.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(74993..76018)
                     /gene="sppA"
                     /locus_tag="ACHBBW_000076"
     CDS             complement(74993..76018)
                     /gene="sppA"
                     /locus_tag="ACHBBW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9151795.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(76031..76321)
                     /locus_tag="ACHBBW_000077"
     CDS             complement(76031..76321)
                     /locus_tag="ACHBBW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151796.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            76731..78536
                     /gene="glmS"
                     /locus_tag="ACHBBW_000078"
     CDS             76731..78536
                     /gene="glmS"
                     /locus_tag="ACHBBW_000078"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9151797.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(78608..79963)
                     /gene="glmM"
                     /locus_tag="ACHBBW_000079"
     CDS             complement(78608..79963)
                     /gene="glmM"
                     /locus_tag="ACHBBW_000079"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9151798.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(79987..81156)
                     /locus_tag="ACHBBW_000080"
     CDS             complement(79987..81156)
                     /locus_tag="ACHBBW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9151799.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(81153..82040)
                     /gene="cdaA"
                     /locus_tag="ACHBBW_000081"
     CDS             complement(81153..82040)
                     /gene="cdaA"
                     /locus_tag="ACHBBW_000081"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9151800.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(82229..85909)
                     /gene="nifJ"
                     /locus_tag="ACHBBW_000082"
     CDS             complement(82229..85909)
                     /gene="nifJ"
                     /locus_tag="ACHBBW_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9151801.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            86107..86628
                     /locus_tag="ACHBBW_000083"
     CDS             86107..86628
                     /locus_tag="ACHBBW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9151802.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(87327..87892)
                     /locus_tag="ACHBBW_000084"
                     /pseudo
     CDS             complement(87327..87892)
                     /locus_tag="ACHBBW_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88071..88727)
                     /locus_tag="ACHBBW_000085"
     CDS             complement(88071..88727)
                     /locus_tag="ACHBBW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151803.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(89295..90230)
                     /gene="whiA"
                     /locus_tag="ACHBBW_000086"
     CDS             complement(89295..90230)
                     /gene="whiA"
                     /locus_tag="ACHBBW_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9151804.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(90264..91262)
                     /gene="yvcK"
                     /locus_tag="ACHBBW_000087"
     CDS             complement(90264..91262)
                     /gene="yvcK"
                     /locus_tag="ACHBBW_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9151805.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(91259..92143)
                     /gene="rapZ"
                     /locus_tag="ACHBBW_000088"
     CDS             complement(91259..92143)
                     /gene="rapZ"
                     /locus_tag="ACHBBW_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9151806.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(92278..93009)
                     /locus_tag="ACHBBW_000089"
     CDS             complement(92278..93009)
                     /locus_tag="ACHBBW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9151807.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(93013..94278)
                     /locus_tag="ACHBBW_000090"
     CDS             complement(93013..94278)
                     /locus_tag="ACHBBW_000090"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9151808.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(94541..97357)
                     /gene="uvrA"
                     /locus_tag="ACHBBW_000091"
     CDS             complement(94541..97357)
                     /gene="uvrA"
                     /locus_tag="ACHBBW_000091"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9151809.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(97367..99361)
                     /gene="uvrB"
                     /locus_tag="ACHBBW_000092"
     CDS             complement(97367..99361)
                     /gene="uvrB"
                     /locus_tag="ACHBBW_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9151810.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            99620..101122
                     /locus_tag="ACHBBW_000093"
     CDS             99620..101122
                     /locus_tag="ACHBBW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9151811.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            101124..>101832
                     /locus_tag="ACHBBW_000094"
     CDS             101124..>101832
                     /locus_tag="ACHBBW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9151812.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTK"
BASE COUNT        28161 a        22660 c        18092 g        32919 t
ORIGIN      
        1 tcttatattt gttataaata aactttttaa atccaacatc attttttaaa gaatcgttat
       61 cataaatctt aggatatttg gaaacaccac atcgaagaat aaatgacaga aaatctctct
      121 cctcagcaaa actagagcaa tctatttttt tcaatgccgt gtaatctgta tcttgaaaaa
      181 gcttatctcc taattcattg ccatactctt ttccaatata cattttattg gaaggttgaa
      241 cgtcaccagc aacgtttgga agattaaatg gaactttaga cagctcactt ccagctgata
      301 gtttttcatt gtagttatca taaaaccaat taatgaaact gatagatttt tcaacagtgt
      361 caatttgtcg attaatcgtt ccaatgatca aatctgaact gctttgctca gtgaattcaa
      421 ttgcaagata tttttcactg aatgcatcta gaatacgttt atctcctgta cgttcagctt
      481 tgtatgcatt agctgtttct tcccatttat ttttccaact tgatgaagtc ttaatctgat
      541 aaattaactc atcaacatat gtttgtttca aaatgcttaa actaacccaa gaagacagct
      601 cttttggaag cacagtaaca cctgtatcgg tcggtaagaa taccttatct gatttcatca
      661 accaattgtc actattgtcc tttaatagct tagggacaat cggagaactt ttatattgct
      721 cactccacca taagaacact tttgtcctta ttctattttc ccaagaagat gaaagaatgt
      781 tgattttatc agatagttca tctacaacaa acgacaggcc agtgtattca ttataattag
      841 caagttcttg tactagttgg tcattttcgt ttgtttctaa taattgcaac aaggtggaaa
      901 atgtctcacc tattagctcc tcagggaatt cttgaaggaa aagttttgga ttatctttga
      961 tagaaatata ttgccaattg acagtcggta gtatttgggc attagataaa atacccaaga
     1021 attcgttcat taagtcaaat acatctaagt cccatagttt attctcttta aaaggagtta
     1081 ccatttttaa tgctatgtct tcatattcag gcttagataa tttttctgct acttcagaaa
     1141 taaatttgag cacctcaata aatattttct tatttatttg ttcactatca tccgggacgt
     1201 catcacgtga attatttgtt ataaacggtg catttataag tgcatgaatt ggtgtaccaa
     1261 aattacgtac tggaaagtag caatatagtt tttctttgga gtaatcgtaa tcagaatctt
     1321 tagaaacggc tagtgcaatt ttagcaactt tatctcctat catagattct ttgtcaaaaa
     1381 caaaatattc ttcagacggt gattgaccag taacatcact tttttctatc aatacatcat
     1441 ttccatccac aattttctct gatatttttt catcatcagt tgtttcaata acaatttttg
     1501 ttatatttgg taaaaataac agtgacttat aaaatggctg ctgaagttgt ttagaaacac
     1561 tcgtgccttt acttaaatta gtttctttta gttttactcg aattaatgta tcaaaaccag
     1621 aatcaatttt gtttattggt tgaggacagt tcattattgg aaaacataaa tcataatctt
     1681 cttttttcca atttttcttc tgctcaaaaa ttaattcact gaatttgtca gaatctcttg
     1741 atacatactg ctcaaaaagc ttttttgtga attcttcact aaatcgaata gcaaattctc
     1801 ccgaatacag ttcgacccac tcagcatcgt ttagcaagga acgaaaacca gtccccttat
     1861 ttccgatatt ttgtgatgat ttattactcg ctcttcctaa gcacaatctt tcaattgtat
     1921 cttttgaaaa tgaagtgcct gtattcccaa cttccaagat attattttta tatgttatct
     1981 gaactttcac ttcgtcattg ctagcatcat tttctgattt tatatctcca taggcatcgt
     2041 ctacattctg taataactct aaaatctgac gcccattata atcacgcatc aattcatatt
     2101 cttcgctgca ggccttttca agttcattac tcaactcaga atccgtatat ttacgaataa
     2161 aaatatccct taattcatca atggtattcc attggctcac agaacttcgc ccccagccta
     2221 ttttttacat aatatattat atcatcaatc actttatgaa atccaatcaa taaagacgtt
     2281 aatatggcta cccacattaa cgtctagtcc tatctattca aattaaattt ctacagtcac
     2341 tctcacccca cttttgaaat caacaacaat gttcttttca aaaatggtga ccttctcaat
     2401 caaccttcga acaagcactt cattatactc attgatgaca tcgtgttctt ttacaaagac
     2461 taccatctca tcaacccgtt gttgcaaatt agtattcgct gctatttgtt ttgcaaccgc
     2521 atctcgttca tctcttagtg aaaatatttg gcttgccaat tcatcgtaac cctgaccgcc
     2581 aactgccagg ctaataattt cagcttcaag ctctttcaac tgactgtcta gttctgccaa
     2641 tcggtcagtt aatccatcag tcataactga gttgatattg tcatttaatt gctgaagaaa
     2701 agcatcacga tcgattaacg tttcattgat tgccttgata acagctccat gcaggtctgt
     2761 ttcgtagatt gttcgacccg tgcaattatg ttctcgttta tccactctgg atttgcatcg
     2821 ccacacagtt cctcgattct tccaatgaac tctgcggtaa gcattgccac agtcaccgca
     2881 aactgtgata ccagataacg catacttccc agaaaagcac tgtgactttt tgtttttcat
     2941 tgatttacgc cggtctagtt cttgctgggc ttgcatgaag atatcttttg ggataatggc
     3001 cggatggcta tcttcaacat aatactgctg agcgtaactc ccgtcattct tcactcgttt
     3061 ctttgtcaag aaatcggtag tatatgtttt ttgaagcagc gcatccccca tgtacttctc
     3121 gtttcgaaga atcaatctga ctccttctcc tcgccagagc tttcgcttcc ctcccgttag
     3181 gatgccatca gccattagac cattagcaat ttcttgcaag gaactgccct caaggtattc
     3241 gcggaagatg cgtttgatga cttctgcttc ctttggttca atcaccaagt taccatcttc
     3301 gtccttagtg tacccaagaa aatgattgtg gttgatacgt agctgtccct gctggaaacg
     3361 atactgtacg cccatcttgg tgttttgact tagtgactgg ctttcttgct gggccagact
     3421 tgccataatg gtcaaaagta cctcaccctt cgcatccatt gtattgatgt tttccttttc
     3481 aaaaaacact gcaatcttca gttcttttaa ctgtcgtatg tattgcaagc aatcaaccgt
     3541 attacgggca aagcgtgaaa ttgattttgt gatgatgtac tcaacttcgc cgttttgaca
     3601 cgcctcaatc atccggttaa aaccgtcacg ctttttcgtc ccagttcctg aaatcccttc
     3661 atccgcaaat attcctacaa gctcccaatc gggatttctt tgaatgtagt ccgtatagtg
     3721 ttgaacctgt gcttcgtaag aactcgcctg ttcctccatt tcagtagaaa cacgacaata
     3781 ggctgccact ttcaactttg gtttctcttc ggtattctct gctgcccatt gtctaggctt
     3841 cattggaata accgtcacct gttttcctgc catttcactt aacctccttg atttttgaat
     3901 atgcatattg cgcttgtttg aatgggtcag aatacttttc ggtaatcttg ccagatttaa
     3961 aatgagtggg tattaccttc gtatctggca ctttgatctc cttgcgtttt ttaaagaact
     4021 taattcttcg ttcttgctcc agatccgctt ggaagaaaat atcctcatca atgatttttg
     4081 gatatatctc atttcctaag taaacctcat tcatcaaaat acgtttcata aagggatgcg
     4141 gcttgttaat gccagagcgt ttgccagctt cttgtaagcc aacaccctca aggtaaatat
     4201 tgaagaactc acgtatttgt tgagcttgct gttcatccac gttgatttct ccattcttaa
     4261 tgacataccc gtatggaatt gtattgttca ttctggtaac ctctctttca aaagtagtcc
     4321 gcacttaagt tcaaatccga cttcttggcg gttgtaaact gtaatgctgt ttgtgaactt
     4381 cttgaataaa tcttcatccc agtccgtcaa catctctgcc atccgcgcga atttgtataa
     4441 ggcttctagt tcaatttgct gagtgctttt gccgttgact gactcaatga tgaggcgctg
     4501 cttttcttgt gcgacagccc tctcttgttg aagtttgctt tgttcttcta aagacacctt
     4561 cgttgataaa atactggaag cagtcaaact tgccagaacc tcaagctttt cttgaatctt
     4621 gtcgatttca tcagcaagca cttcgagccc ttcctgattc gcacctgctt tattcaaaga
     4681 cttcataaaa ggttccagaa ccaacttgtg tccgaaaatc aacttattca acatggtcaa
     4741 ataagcaacc ttcaaatctt catccgtaat tggtaatgca tcgcagctat ctttattttt
     4801 aagatgcccc gtacacttcc actgaacctt cttcgtatta cctttccaat aagtgtgaac
     4861 ccgcttgaat ttgctgccgc atttatcaca gatgatgttg cctgaaaatg cgtatcgctt
     4921 ggtgtacttt ttcccgtcac cagtattccc tttggcctca gcttttctcc gaatgagttc
     4981 ttgaactcgg tcgtaatctt ctttagatac tatgggttcg tgattgtttt tgatctggta
     5041 cattggcaat tcgcccttgt ttatgcgtcg tttgtactgg tcatcaacaa aggtttgttg
     5101 catcgtcaaa gtcccggtat aaacttcatt tttcaaaata cttctgactg atgttgcttg
     5161 ccaatttgcc ccacgtttag tgaattgttt tccatcgttt aaccgcttgg caatcattcc
     5221 agtcgaaatg ccatctaatg cccactggaa catttgttta acaacttttg cttcagctgc
     5281 aacgatgtga aaatcatagc ctttccgctt gtagccatac ggcgggtaag ctttgaattt
     5341 ccctttagac atccggtttc gaatcgccca cttcaagttt tctgaatggg aataggattc
     5401 actttctgcc aagcttgata aaattgaaag caacaactcg ctttccatat caccagtatt
     5461 aatgttttcc ttttcaaaaa agatggtgac accaatatca gttagctgcc gaaccagatt
     5521 caagcagtca actgtatttc ttgaaaatct ggaaatagac tttgtcagaa tcaaatcaat
     5581 ctttccaagc tctgcatcct taatcaatcg ttttaaacca tcacgtttta cagtcttggt
     5641 tccagaaatc ccttcatcat aataaacacc gacatagtcc cattgttcat ggttcttgat
     5701 gtgattttca tagtgagtaa tctgggtttg taaacttgcc agctgttcat tacttgatgt
     5761 tgatacacga gcgtatgctg ccacctttag tttggaagct tccggcaact ctggacgtgc
     5821 caatttatgt atcgttttca tcagctcaac tcctttccat taggtgacat gttagctcta
     5881 aagtgccgtt atatcaacgt ttcagaccat tatccttgat aaaaaaggat tgaatgtttc
     5941 ccggttttta ttgtcgattt tgacgtattc ttccaggttg attttaccgg taacaagtaa
     6001 gttcagaagg atctgctgtg aaatgtaata ggctgcttca tctgaatagt taatttctgt
     6061 tttcttgtta gtcattgctt tcgcctcctc aagatacagg cgaagaaaat ccatgaaagt
     6121 aaacccaatc acaaattttc ctttgccttt catccatatg cgaaagaaat aaccgactcg
     6181 aacccacaaa acgaaaaaag cctgcaagaa tgaaacgaat cactcttgca ggcttctaag
     6241 tttgtttata tatagtagga attattcttt gtaaattttg actttttact tcccaaacat
     6301 caaataagcc tttacaagct tcatccaata aataacaata taaatatcaa ttgtgggaag
     6361 gagaaagtta tgagtagttt tgatatggaa gggttagaaa aagaatatga acatttttgc
     6421 aaatctgcga caacaaagga actggaggaa tttgatgaaa gcttcaaaca agaatttgaa
     6481 aagtgcttta aaaccataga ggctgaagaa gctttatacc agtttttaca agccaatata
     6541 aacgcgatga ataatcgtat ggaatcaata gaatcgcctc ttttctgcat caattacaaa
     6601 gagcaggtat ttcagttaag aaatcattta atcctaatgc aaaatgctgt aaacacctac
     6661 cttaaagaaa actagacgaa aaaaaggccc cacaataaaa cttgtgaggc tgttttgaat
     6721 tataaagacg tcaaactact tattgaaaat tttgtagctc gttggaacca tcgtgaagtt
     6781 ttcttcagta attgggccat cataaatttt gattttgcaa acttttgacg ccacatcaag
     6841 gtcgtgaaca aagtaaattt tatatggtgt atctccaagg gaggcagcaa cctcattagt
     6901 actcatttca aatccgtcag agaagtttgt ttttgaagct ttcacttcaa tatggatttc
     6961 attacctgat tggtcaaacg cacaaatatc atagccatga ccatctcctt cagttttaga
     7021 aacatgttca ggaagttttg ttttgttctt ttctgcttcc tgaatgagga aatcaagtac
     7081 gatttcttcg cctagagcac ccgttttttg ttttgccttg ttgacacgct cccaatcagt
     7141 atggcgtcca gtgaattttc gtttctgagt cccattcgtg cgatttggtc ccacttcatc
     7201 aacaagaaca gtaaatcctt ttgaactttc ttgctcaatt tcttcaactt cctcgtttgt
     7261 aagatccagt tcagacggca acgattgtgt atcatcgatt gggataagat gacgattttc
     7321 aatcaatgca acaaaatcgg cataggtaat ttcattcatt ccatactgat cccagaagta
     7381 ggcccatgaa ttcggctttt tacgcttctt tttgctaggt gtattccaat cgaagttttc
     7441 aataaatgaa tcaccttgtt taattgccgg ttcgattgta aatgcgtgtg taattactgc
     7501 cgtaacattt ccagcttcat caggttcaga aatgctctca acgtatccgc ccccgtaaat
     7561 ttcccactta cggtctttag taattttccc cggatgacga ttcaaaacaa ccgcgccggc
     7621 tttcaattta ccatattggc gcatgttcca accgtaactt ttagcatcat cagcgtattt
     7681 caactgacca tcatcttctg ttttcccgtg atctaaaatg aaagcttgtt ggctttctgt
     7741 tggttctggt aacaaatctt taaacgtaac catatcgata cctctttcta taattcaatc
     7801 ggaacaaatt tatgttctgg ttgaattaat aataacacgt tcgatatttt aagtcaacaa
     7861 tccggacata attttgttcc gattaaattt atgaaatcaa aatataatcg tcagattccg
     7921 tcttaccagt attcttaccg acagcacaat atccaatcgg gcaacgagcc catacccaac
     7981 catccgcaat cgtcatcttc tctgacttcg gaagcgtcca agttgcgcct ttattgtatg
     8041 aaccaacaac cgcaccactt aaacttggct tgttgcgaac gttgagacca ctcacttgaa
     8101 ccacaagagt tttcacgttg gatgttgatg acccagatgg ataaacttga ttaccgttct
     8161 ggtcgaacac cttataacca gaatggctat ctgcagcccg cttcgcattg ttcaagtcct
     8221 tataggcacc aatttgtgac ttggcgtcag cccatgattt gcgaacacga tacagaccac
     8281 tagctggcgt gcttggggtt gatgaaccat tattaatcgc attggcttta tccgcaatct
     8341 cctgcagttt accaagcaac tgtcccggac atgatgtggc cgaaaaatgt gagtgaggga
     8401 agatattctt gttcaccacc aatttcccta gaccataacg tttggcaata tctgcacaca
     8461 actttgcact tgcatcaata gttgcttgat tgatacccca gttcggtgat ccagtgctat
     8521 tgcagtgctc aatcccgata cttgagatat tggcgttcca attaccagcg tgccaagctg
     8581 tgtttccttc gtccacgtat gctcgaattt ctccattttt tccaataccg tagtgtgctg
     8641 acgcctcacg cgttttccaa atatcaggaa caatatcata gttggtggtt gccatgtgat
     8701 gaatcactat cttatctact ttggcaccac tacgaccatt cgagtaatgg ctaaatgact
     8761 gaaatccata ttttgttgct agattactaa ttcccatttt ctgtttcctc cttcttttcg
     8821 tgtaactgct gcaagatttc acgcaacttt tccggcactg gcagtccaat cgccacggca
     8881 ttctccagca gtgaaattcc ttcgtttgag atatagaaga aaatgaccgc atcacgcagt
     8941 acatttccac ctttgatgac ttccacatca agcatatgag ccacaccaac caaaagaaaa
     9001 atcatgattt tcttagtgat gccctttgcc ccaatcttgc tggatagttt cttttcgttg
     9061 attgcacgaa gcagaccagt tacatagtcc actaccacaa aagttacaag cgtataaatc
     9121 aaaccatcaa ttcctcccac ataccatcca atcactcctc caccacctgc gatggcgagt
     9181 tgaatccaat tccacacttc tttcatttac aaatcctcca attgttctaa aattaaatcg
     9241 agcatttcag catctttccc tgaaatggtg acttcactat tttcaaatcc agcaaccaat
     9301 aaaggcagct tgtcttgaag ttcatccacg ttgatgtggg tttcttcgtt gagcaagtct
     9361 gccttttctt tgtgatattc agttgcggtt tctgggatga gtgaaaaggc accgtcatgt
     9421 tggaccaaat tgccgtgctc atctttcttt ccgtattttt gaagcaagtc tacctcgtct
     9481 tgatagagtt catcaacctt cacctggatg agttttgcaa gcttcgttcg cactcggcta
     9541 atctttggtg gtaagtcaaa accttttaag aaactcatca cgtccaccaa atcttcattt
     9601 ctaattttga gcattatcta tcttctcctt taaatcacca atctggacac aaagatcatc
     9661 cacgaccatc acaagcaaat caaccatatc cgtcccaact ttcattggct ttggaatatc
     9721 tgcctcgata ggttcagtta ctatcacttc ttctttttct tcgttcatgg cggcctccta
     9781 actgtatgtg gtcattgaat taaacgactg cggattgtac caaccactcg ctgtaccgtt
     9841 gctactgaaa ccggttggta aagcgattgt tcgtcccgca agtttggcac agatttcacg
     9901 gatgactcca aagtcaaccc acttcccatc atctccgagc atcagattag aaccgttgaa
     9961 tgcaatacca gcgctgccgg aacttgagcc aatcttagta taaagggaac tactaaatgt
    10021 aatcccacca acagatattt gatgttgtgt ccctatacga agcattgatg gtaaataaat
    10081 gtcgtcgtca aagtggaacc ccttctccat accagactgc aatgttctcg cataccaaga
    10141 aagcttctgg gtgtagacac tcgcagagga ggaatcttta tgagcccatg acatatactc
    10201 tgttccatac tcaagatcaa ataccaatcc acgccaatca gagtgaccac tgatgttatt
    10261 tgcaccaatt gttccgactg tcgttccacc acgtcgaatg gtcattccct gcgagtttat
    10321 ttcagtcaca agagtgcttc ctgaataata tccaatcgta gttcctgtga ttcgaatgga
    10381 cgaactcgca gtcaatgctg ttaggatatt agcagcaatc ttatcagccg taatcgagtt
    10441 cgcgccaata cgggctgccg acaaagttcc tgtggtaatc ttcgccgcat caatgcttgc
    10501 aatttttgca ctttgaattg tggcatctgc aatctttgcg tttgtgatgg ccgcattttg
    10561 gatcatggca gaactaatca ctgcactatc aatggttgtt tgaccagtga tgtgcacctt
    10621 gtttccagca atcaaaatac tttcaggtga gatattgatt tgattaatca cgtctccttt
    10681 accaactcgt aaattgatta agtctttctg catgagaatt gctgaatata atgtgttggc
    10741 attggtatag tcttgccttg tggtcgtagg ataaatattt ccttcatatt catcgggttt
    10801 gatgctcgtg ttcactcgga ctcgcatttt caatgtgcca ctcgggactg taattgctcc
    10861 gctcgaacca gtataagaga tttgatgata acttgaacca tccgacgggt ttagagtaaa
    10921 aggtgtgttg cttgaattat aaatcccagc atatgtgcca gtcttaactg aaaagaaagc
    10981 tagatacacg ctgtcagtag taatccctga tatctgctca gccgttaacg tccaagaata
    11041 agtggcattg ctgctacttg taacggtcga aatctgttcc caagcaccac ctgtataaag
    11101 caagacaata ttatctgatg tatccttgcg accattccac gaaattacac tcggagcgac
    11161 tttgccttcc aatggcaatt tgtaaatgac cagataatct cctgaatttg aataggggtt
    11221 tgttgtcacg ccacttgtaa tactcatcga ttcaacgttc ttacttacta gataactcgc
    11281 aatggtcgca ttagtataag ttcccgccgt tagggtttca ctaaattgtt ttgccgttgg
    11341 gacaaaatca atgtaaggta aatccatgcc gtagtaatga tagtagaaag cagcttgagt
    11401 tccgtcacgc agttgcccga tatacttatc tcccactttg acatcaagga actcacttcg
    11461 gagataattg gttgcggaca cttctgaacc gtcagtggta ctcaaacttc cttgttccca
    11521 ttgattgact ttaaatggaa cgccgattgc ttctttggtt tctgaaacac ttaacagtaa
    11581 ggcatcagtg gtcgctgtaa gttttacgac gttacttatc tcggataaat caacatagcc
    11641 atttgtcaca tccgttttca acatatagca actgatggtt tctggtgcat cactagttgt
    11701 taaacgaatg cgaataaaag cggcactgct tggaacagta agcggtgtgg tggtgtttcc
    11761 aaattgggtg tacgataaaa aggacttatc ctctttgtaa taaagcagat aagccgttgc
    11821 gtaagtagag taaagtttcc cacccacaaa tgtttgaagt agatactttt cgccatttct
    11881 aactcttgta aaacctgagc gaacgtatcc accataggta ttttcttttc catcatttgt
    11941 attcagacca ccaactgccc actgcaaatc tgcaatgctc ataccggcta aaacattgac
    12001 cgctttattt gttttagaaa gagatagctc aaatctatca gcattttggg tgatggtgct
    12061 ttctgcagtt gtaatacgcc ctgtcagatt atcaaccgtc gattgacttg ccttactagc
    12121 aatctgccca gcttgcactg tcagagttga ttcagctgtg attactcgtc cttcaagagt
    12181 tgacatacca ctcgccacta atgaaatctt gctagtattc tgttcaacgg atgtagtaag
    12241 cgtttcaaca cttgcaccta cactttcagc tgcattcaaa gcattcacag catcgttaaa
    12301 tgcttgattg gccttttcag tggcagtatt ggcatccttt tgtgcttgtt ccacggcttg
    12361 ctttacttct tctgccgtca tatctgtcat aagtggatac cactgagtga cgccatcacg
    12421 gatttcatac acccacattt caaacttgtc accattttct ttgtaccaaa cgtcaccgag
    12481 tttcggatta tttggagttg tcgggccata gaagttggtg tttttaccat ttgccgaact
    12541 caatgcgtaa ttggcatcgt ctttggcttg ctccgccttg ttgtccaccc ggttgatgtc
    12601 cttcgccaca tccgtgaatt ttggcgtgat gtttccgagt gttactgaga tatacgcttt
    12661 cagaaatgga tcgtatttgt atgagaccat tctagctgtg atgttcaatc catcttcggc
    12721 atgaataact gaaacagtat cacccaaatt aatcgtttca agctgtgcaa aatctttata
    12781 ttcttcagtt cgctcaagcg gggcaaactc cacatcatag gtggcttttg gcaaatcgac
    12841 cttgttagct gagtattcta acccagccaa tctgcgaagt tcatcatagg cctcggcttg
    12901 agtaaactca tcctcttcat caccaacttt gacgttgtcg tacttgatga ctttgatttt
    12961 aggctggacg tattggttga tgtttggact gtccacgtat ttttcgggaa gaaatagtcc
    13021 atcataccct tgtggcataa tgcgagtaac aattccagag taatcaacat cagctttata
    13081 tcctgtcaga ttcttcttgt ctcggatttg tacgccgttg ttactgccac gagtcgttaa
    13141 cattgaaatc gtgaagttat cgcgtacaat ttcaccacca taactgcttt gaaaaccatt
    13201 ctctaggtca ctatcaagca acacctctac aggatttagt cgcaccaagc ggacattatt
    13261 gaccgcactg atgtttgatg taccggtaaa acggtgcatg aattgagttg caccaagcag
    13321 ttgtgtcaaa gctcccgacc cgttcttgtt gacgatataa gtatcttcaa taaagtttgt
    13381 ggctaggtca tagaaaatat gatgagctac tatgtgcaaa aaaccaccaa gtgcatcatc
    13441 tcgttcaaca atcctgaaaa gctggtcgga cagttctggt actggtgctt tcacaatcat
    13501 ttctggcaac aattcctcac tgtgtggagc atgaattgga taatcgaatt caaaggaaaa
    13561 gagtccgttc aattcctcac tcaccacggg gttcagaatg ttgccatcaa ggacagcgag
    13621 accgttatga ttgaaatttg attctgtttt gttgtaaagc gtaatcataa gtatcgccac
    13681 ctcggttcta tttctacttt ggttatacct gtaccaagag tcacagtatt actgccgact
    13741 cgaaactcag gaaagtcacc tgtcatgtat tgattggctg ccacatcccc gtaaaagcat
    13801 tctttcaaat ccgaatctag tgtcaggttg ccattcgtca gatttaacac gataggcttg
    13861 ccatttatcg tgagtgtcct agttccattg ccgtagacct taatcttcgg caatgagtaa
    13921 atgttgccgg gattattgac cgttcctgac gtggttcgat tgatagtcgc tacattggtc
    13981 ttataacgaa aaggcgaaca ggttaactcc atttcaaact cccacatgga agataaaatc
    14041 tgagttagtc cgctcgcctt agcatacttg attgggtaaa acacgtttgg ttcatttgaa
    14101 aatgcgatag ttttagcgtt taggatttgt ggcaaaatta tctgccagct agtccaaaca
    14161 tcagatttcc aaatcactac cttaaaaccg aaagtcacat ctttccagcc tttcagaatg
    14221 gtcaaatctc cttcacgtcc atcaatctca atactatcaa tgacacgttc cgtcactgga
    14281 ataactggca cttccgtaat tcgcaaacct aaatcagtcc gtgaattaat tgttccgtct
    14341 aaaataaatg cgttcaattg accaccgccc atctaaataa ctaaaaaagg accagacaac
    14401 aatgtctaat cctaaagttt ataatctatc atcctattcg gttgtttctt ccagccctat
    14461 tttcttggta atcgctaaat acgcgtatac aaccagcacg ccggaaattc catatattgc
    14521 atcgattaga tctggtgtat tcagaaaagt catcatcgtt tcacatacaa tatttgcaac
    14581 aatcagacca gctcccatcg cagtaagaac ccttaaattc gttcccataa gcaaccaaag
    14641 atagcctatc accaaataga aaagaaagct taatgcgaag tttgaaatgt gagaattaat
    14701 ctgattgttg atgaatacaa aaccatgatt tatgccgcta taccatctta ctgccgttaa
    14761 aaataatcca atagcaagca gtagaatata tcctatcagt gcaggtttct ttgctttaaa
    14821 tttatgcttc aacatatgat taccaccatc tcttcagtaa acactcgtta gttatagtat
    14881 acagtcaaaa tgtgaaaatc caacactaat atgctacacg tctacgattc aacgtcgcca
    14941 agctcttatc aatttctgga gcaagtttgc cgaccaatgt tccatcattc aagacgactt
    15001 taatgtccat tgctttaagt agcgttggca acatttcact cgtgatgttt gttaaggcat
    15061 caaccttagc acctaaatca agcatcgaga atgtgctatc aacggcagcg tggatatcgg
    15121 tatcaatatc aaagcttggc atataaagtt ttgttggaat agctttctgc atcgttttag
    15181 aaacaccagt catttcctta tcaaaaccta cgccaatccc ttgtgccatg tacttcccga
    15241 tttcatcacg aaacagtgtt gatggtgaat gaataccaaa gaagtccttg atgttgtcta
    15301 ctacaccgcc aaagaatcct gaaatcttgt tgcgtaacca acttccagca tcagcgatac
    15361 cattccacaa tcctgtaatc aagttcttcc cgatagttcc catagttgaa acatactcac
    15421 cgaaagcatt cgcaagccct gtgatgattt gcggtactgc tttgacaatg gcagaaatga
    15481 ttgctggtaa gttttgaact aatgccacaa gcaacttcac accagcattg atgattttgt
    15541 cgatgttgtt taccaaagtt cctgttattc cgttgataat ttgcggcatg gcattcgcaa
    15601 tcgttgtgat aatttgcggt aacgcaccaa tcaatgccac aagtaactga atacctgcgt
    15661 caatcaactg agggattgcc ccaatcacgg cattgatgat attttgaata atcactggaa
    15721 tagctgcaac aatcgcattg atgatatccg gcaaagctgt cacaagtgat gtcaaaagtt
    15781 gaataccagt ctgaatgatt tgtggaatag cactgataat aaagttcacg attcctgtaa
    15841 taatctgtgg cagactttca atcagtattg gaagtgctgt gataagtcct tgggcaagtc
    15901 ccatgataag ttgtaaagca gcatccaaca gtaatggcaa gttttcaatc aagccgttca
    15961 caatcgttat gattgcctgt actgtaattg gaataagttc tggcagcgct tcgcctagtc
    16021 cttggataag tgttgtcaca agttgaaagg cagcgtcaat cagtaaaggt aagttctcaa
    16081 taagtgtttg aacaatcgtt aaaacggctt gaaccaccac tggaataagt gtgggaagta
    16141 agccaagtat cgtttcaagc acttgtgaga ataatgaggt tactgcttct aagagtgttg
    16201 gcaataactg ccccaatgct tgaataatgg catccaccac tgccggtaaa gccgacacga
    16261 tattttgaat gaccggtaga atattcttga tgacgttttg aaattgttcc accacattgc
    16321 caatcaacat accaatgtca gcgctgctgt taccaagtcc agccatcaag tttgagattg
    16381 cagacttcat accgtcaatc gaaccactta tcgtctcact cgcttccttg gcggttgtac
    16441 cagtgatgcc catttcggtt tgaataacgt gaatcgcttc agtcacatca gcgaagttcg
    16501 agatatcata tttgacacca gaaatcttct cagcctcttg gagcaaacgt tccatttcgg
    16561 acttcgtgcc cccaaaacct aatttcaagt tgtccagcat cccgtaattt tgcttggcga
    16621 aaccttgata ggcgttctgg atggactcaa tatccgtccc catcttgttt gcattatcgc
    16681 tcatatcagt aacagcttga tccgcaactc ctgcggcttt cttggtatca ccaccgagag
    16741 atgaaatcaa ggcagctgag aaacctgtga cagtttccat gtattcattg gctgacattc
    16801 cggcagtttt gaaagcattg tcggcattct tttgaacggt ggatgacgca tccttgaaaa
    16861 gagtatccac cccaccgact aattgttcat agtccgcgta agcagatacc acttgttttc
    16921 ctagctccac ggcagcagcg ccagcagcaa gtgccactgt acccattgct accccgatac
    16981 cttttagaac gccaccgaac ttttcaaatt tacttccaga cttttcagcc tcgtcacctg
    17041 tatccttgag ttcgttgcca agctcatcag ttttcttagc agcatcaact tcttcagtac
    17101 ccatggcgtc aattgccttc tcgttgttag caacttcacg ctccatatta ttgagttggg
    17161 cttttgcatt gtttaattgc actgtccaat tctgagtacg cttatcgttt tcaccaaaac
    17221 tgtcactagc gttcttcaaa gcattttcaa gggtggtgat tttttctttc tgggcatcaa
    17281 tcgatttgtt taagacttgg ttgcgagcag acagtgagct gatgctttta tcgtttttat
    17341 caaattcgga tgataccagt ttcatttctg aacctagcac cttgaatgat tggttgattt
    17401 cacgcaacga gttcttaaac tccttttcac cttcaacgcc aatctttaaa ccaaaattat
    17461 ctgccaaata aatcaccacc ttcccttaaa ttccactcgg tatcacatca tcaatgaata
    17521 actcaacttt cggcttgcta atcccagtaa attgcttatg acactcccat aaatccaaaa
    17581 acagtccaag cgagcagtac cagaaatcat ctgacgtcat acccatttga actgtcccat
    17641 agtaatacag gcgagtaaat gtttcttcat cacttactcg cctgctttgt tttttgaatc
    17701 tgtttcactt tcgatattgc gagctgtgcc cttgaacatt gcttcagtga ttgctgattt
    17761 atattcagcc aactgcaacg gactcgtcat caactccaca tattcagtcg tgagttcatc
    17821 tttcttttct gtgttcttca agttgtaaat cttgatggac tgattcgcca acagcgtaat
    17881 caaccagatg atttcatcaa gcgccaattc aaagttctcg gatttcaaca atttctcacc
    17941 aaggttatca agcccaccgt agtgactggc gatttctttc gtggcttttg ttgtcaaaat
    18001 cagttcatat tcatctccgc caaggctaat cttggcactg cgttcttcac tcattgttgc
    18061 ctcctagtct gctgtgaaat ctggttcata aacactcgtg taccatccat tgatagttgt
    18121 ttgttcaact cccgtatcgt tttcatcaac ttctgctttc catggatgct taccactagc
    18181 atctaacttg ttacgacgca tgactgtacc ctcaattgtt ggtgttgaga atgtaatgtc
    18241 gtcacctttt gttgctaggc ttgtgctggg cacggcaaac ttcactcggt acaaccacaa
    18301 atatttgtat ttgccattcg atttacgagc acggaaacca atcgcaactg gtgtgccacc
    18361 atcttcggtt gtcgaaatta agacaccgtt ttgatccacg gttgcaccaa caagcactgc
    18421 cgccacagca cgtccaatat catcaacacc gagcgttaac tttccatttt taaattcttt
    18481 tactacttct gatgcaccat catcggcgta taaagttgct tcagcaagtt ccaccgaaag
    18541 ctcagctgag attgctttcg caagcatagc gggtgtgccg tatgtttctt cgccagtagt
    18601 tgccgactct gtaatcggtg cataatataa tttatccaat ccaatcgttg ccattttcag
    18661 acctccattt cataatgctt ggcgacatca atcgccacat gatagtaatt tgtatctttt
    18721 tcaaatccta caaataatcg ttcagtaatc gtaaactcgt gtttcaacaa gttctgaacc
    18781 agttgttttt gcgtgttcag atagttgcct ttcacaaaca aggaaatgcg aacttcctgt
    18841 acatcaacgg ttggaaagtt atccccgtaa acatcgaacc tgtcagcgag tggtgtcagc
    18901 acacaatact tgtctggcgc tttatcacta aactcgcccg tttcaacagg aatatgaagt
    18961 gtggcaaaaa gactgttcaa ttcctctaaa atgttcaaat gccgtttacc tcactttcca
    19021 tttttgattt cattgtttca atgcatgact tgcgactttg tgattttgca ggtttcaaga
    19081 atggctttgc tggctgaccg tgttttccat attcgagaat gttcgcaatc ttagcatttg
    19141 aactgccatc gattcgaggc tcatcaaaac caaccttgat gttccagttg ccgcttttat
    19201 cttgacgagc ttgtgtgata ccaagggctg attcaagttc accagttgaa cgacttggta
    19261 cttttgtatt tgagccaaca acggcagtta agttacttcg gactttgcct tccaccactt
    19321 cagcaccact ttctaaaacc ttggggatga tttcatctgt cttgttttct agttttgaaa
    19381 cctttaacaa gaaatcttct ggcatcttca tcaatgcttt agccatccgc actcacctcc
    19441 atttcagtag tcagcatttc aaggtacata ttgcgatttc ggacattctc caccgacaca
    19501 atctgatacc ttttctgatc tgaaatgaca accatctctg tcgtaacctc tagattagga
    19561 atataacgaa tccgaaacag gcaggtcgct gaactaaaag tcgccatgtt tacccatttc
    19621 tcagtcgcat tcttttcctc tttgtatgca tggacagctg caagaacttc atcttgtttc
    19681 actgaaaatc cagcgctatc cttttggttc gtcactttaa cgatttgtat tcgttgattc
    19741 atctttccaa aactcatact tgccaccgcc tatcgagcct gagtaacaga ttgaccgttt
    19801 cccagacttg cttactggca ttgacgttat ccgcaaagaa gccaccagtt gaaccatcac
    19861 ggctttcgta aaagttactg gctaacacaa taaccgcctg ttcagtggtt ggcggcattt
    19921 catgttctga atagtagcca gactcaatat gttgatagct ctcagcataa gaaagagcgg
    19981 tcagaatcaa tcgttccaac agctcatcat cttcgttatg ctcaagtatg aggtttgcct
    20041 tgaccttatt tagtaagtca ctcataggct actccttaag atgaggcaga tgctttcatc
    20101 ttcaagattt gcactgcttc aggtaacaca agtttaccgt ccacacgttc tttagctaag
    20161 aaaccaacca tgccattacc tgcaaagagt tcacgcaact ggtcgaatga acgcacgcca
    20221 cggtcgccaa tgttatagta gctgaaatca ccaaaagcaa tgactggcgc acctgcctta
    20281 acggttggaa caaactccga agtataaact ggataaccga gcagacggtc aggttcacct
    20341 gctaccaaag ctggctgcca catgtaggca ccattgttgt ctttcaactt acgaagcaag
    20401 ctgatggttt ggtcgttcat gataaacttc gcgtttttac gatatggacg tttcaaagca
    20461 tacacaaggt tcaccacatc atctgccgta actgcacttt gagtattagt tgtaatggca
    20521 acttgtccgc cacctgttgc tgcaaaaata ccgagtggtt taccaacacc gtcaccattc
    20581 aagaacgcat cttcttccgc gtttgctaag gctttggtga attggtcgat gatatagttt
    20641 tcaaggttaa aggcgttgtc ataaagcaat tcatcagtca ccttgattgc cacatgaagt
    20701 ttgtgagcat caagcaagat ttgatcgaac gttgcatccc caaagctcaa ctcgccacct
    20761 tcttcaatcc atgaagcggc tggtttagaa cctgcaatat taatcttgtg ttcaccactt
    20821 gtcgtaattg ttgtcgcaag tgtacgcatg atgttttcag aagtcatgcc gtcaatcaaa
    20881 cgtgagtcat attcttctgg caccaaataa ccgcctgccg tatccacacc ttcttgaaga
    20941 acatttgaaa catgtcggaa gtttgagcgc aaggcttcaa gtacaccttc tttatattca
    21001 gctgaagcac gtcctgtttt cttaggttcc attttatttt cagtagtcat aggttgcgaa
    21061 gttaatggtt ggctcattgg cttttcaagt tcacgatcca tcgcttccat tgactgcata
    21121 cgttcaattt ccatcgtaaa gttcttcact tttgtttcca tttccgcata agtcttggca
    21181 tcttcttcag acatcaagcc atctttatct tgctttgctt ctacaaaagc ctttgctccg
    21241 ttccaagctt gtgcacgttt ttccatcaat tcttgaattt tgttcatggg ttagttcctc
    21301 caatttttca ttaaattaag ccgactcatt aacgaatcgg ctgggatagt gttattaatt
    21361 tcttctcgtt cctttggttc aggtggttca atacgacact tcttagcaat cgctgaagtc
    21421 agtgaattag ttactttgac ttccgagtac atcgaattat taaccggaat atccgggtca
    21481 atctcaccga tgatttcatc ggcaaatcct aggtgaacag ctttatttgc gtccatccac
    21541 gtctccgcat ccatcatatg tgccagttgc acacgcgata aattcgtttt acgttcatag
    21601 gcgttgataa tacttgcttt cacttcatca agcatggcaa tggctttctc catatcaact
    21661 ttttcgccaa acgcaaccgc tgacggattg tgaatcatca ccatcgccac aggactcatg
    21721 agtaccttgt cacctgccat tgcaataaca cttgcggccg aagcggcgat gccgtcaatc
    21781 ttgactgtga ctttaccttt gtagtcagca agcatgttgt aaatacttgc ggcagcgaca
    21841 caatcgccac cacccgaatt aatccagact gtaacgttac ctccacctga atttaactca
    21901 tctttgaaaa tctgcggtgt gacttcatca tcaaaccagc tctcctcagc gattggtccg
    21961 ttgaggtaga gggttctctc taggatttcc tcttggttgt cttgattcag aaccgtttga
    22021 ttcttccact tccaaaactt cttcatcttg ttcctcgctt tcttctttgt ttacgccata
    22081 agctgccccg gcttgttcaa ttggcagcat attgccattg aagtaatatt tcgcaccagc
    22141 accaccaggg attgggttca agtcctcaag ttcacgcaca tcatcaggag ataggaaccc
    22201 attttgaata ccggttgcat agccttgttg acgtgtggca tagtcaccac gcaataagcc
    22261 atccacatta aactttatga aatacttttc tttttcatcg cgacttaaca gtgatttatt
    22321 aagtgcttgt tcccaacgat tgagccatgg tgataatgag aacttcacaa aatccaaact
    22381 caaatgttca acgtttgaga aggtcgcgtg gtctaagtcg ccaaccatgt gaaggggcac
    22441 tcggaatatc cgtgcgattt cttccagttg aaacttacgt gtttcaagga actgggcttg
    22501 ttctggtgac atggaaatag gcgtatactt catgccttct tcaagtactg caaccttggc
    22561 cgcatttgaa ctgccaccaa atgtcgcatt ccaactttca cgaagctttc ctgggtcttt
    22621 caaagttcca ggatgttcta ggacaccacc aggattggca ccattggcaa aaaatttaga
    22681 accatattcc tctgttgcaa tcgcaagccc gatagcattc ttagccatgg caattggtga
    22741 ataaccaacc aagccatcaa agccaaggcc aggaatgtga agcacgtcag cttttgaaag
    22801 tctgacttgc ccgacatcag cttgatataa ataatagatt tccttgttgt catcgcgatt
    22861 aaccgtcatc ttatctggca ttaaaggata caaagctaca acttcacctt tgccatttcg
    22921 gataatttgc gcataggcgt taccccaaag tagcaggtga gtcattaagg tttcacgaaa
    22981 tacaaatgat gtcatttccg aatttggttc atcatgcagc aagaaataca acgaatgttt
    23041 cattgcttta tctttgccgc cagcatctcg gtattggtaa aagtgtaatg gcaatcctgc
    23101 cacggcttct gatagcacgc gcacacatga gtacacagcc gtcatctgca tagctgtttg
    23161 ttcggtcacg cttttaccag ctgatgagcc gccaaacata aatcggtatg gattcatgat
    23221 ggtttgattt gttggtttat cgcgtgattt gaatagtgat tttattatgc ccattgattg
    23281 ctcctttcaa aatttgagta tcaaaaaagc acctacgatt tgtagatgct gtgttcacat
    23341 tagatataaa accgacttcc tcttgacaaa attatctcaa ttgcattatt taaaaaggta
    23401 tttacttcct ctaaacattt cgatttttcg tcaggtgtta aggattttct ttgataagga
    23461 aagaatgaac tacttggtgc aagtttttta aacgtcatat aatcttcatc atcgaagaaa
    23521 tcttgtaaat aatttagttc tataaaactt ttccaatctt tgaatttaga gaaatagtct
    23581 ttattattaa cgataaattt atcagcgtta gttgatgaac catatcgttt atctcttaca
    23641 tcagaataaa ataaatcagg aaagtcatga tacggccctt tacctttaac accattgaga
    23701 gaaggttttg atacatatag tggcataaaa tttgcaagag tatgacttaa atcatagaat
    23761 tttacaaatt tcactttcat ctcttcggtg atatcatcgg aatataaaat gactttcaaa
    23821 cgcccataat gactatctgg cattgaaaag ttcttttgat tatagcattt caataaccgt
    23881 atcgttgcac cagcaattgt gttgaaagaa ataatcgttt ctcccctata attcccaatt
    23941 ccttcagcat actttgtcaa atcttcagtt agaatttttc cccaccattt tctataaaac
    24001 agtctagaaa ttaatgtgtt gtccgggtct gaagatattt tatctggtgc tacttgaatc
    24061 aaatattcat tcatacacat acacctccaa agacaattat atcaatggat tgtaataaaa
    24121 gctcatattt cacagaaaca acaatcccct atcatcataa acactggcac cattatcatt
    24181 tccacaacga attgcacgat ccagagccat aattgtcgcg atggcaccat caatcttctc
    24241 agtggatttt tctttatcag ctttgatatt acctgccggg tcagttcgga cgaaaatgtt
    24301 gtccatattc caacgaagaa ccggatgacc gccatgggct aatttctgtt caagagtcaa
    24361 cttcatcaac tctttagtag ccgggctcat atccttgaaa ccttgaccga aaggaactac
    24421 agtaaatccc ataccttcta agttttgaac catttgaact gctccccaac ggtcaaaggc
    24481 aatctcccgg atattgtatt tctcacccag ttcctcaata aatttttcaa tgaaaccata
    24541 gtggactacg tttccctcag tggtttgtat gtacccctgc ttggcccaca aatcataatt
    24601 cacatggtcg cgtttaactc gtaagtccaa ggtatcttct ggcacccagt aatatggcag
    24661 tatcacaaac ttgtcatcct catcttcagg tggaaacacc aaaacgaagg atgtaaggtc
    24721 ggtcgtgctg gaaaggtcga gacctccgta acaaactcga cctctcaaac tttcttcatc
    24781 tgcattgaat gaacaagcgt cccatttatc cattggcatc caacgcactg cttgtttcac
    24841 ccattggttc aggcgaagct gtctgaatga gttttcttca gctggatttt gtttagccga
    24901 atcacaagca gccaccacct tttcttctgc aatcgttatc ccaagtgacg gattggcttt
    24961 cttccaaact tctggatcag tccaatcatc atcttgcttg ataccgtaaa ttaacggata
    25021 gaatgtttta tcatgctttc gaccttcaat aatgtcgaga gctttttgat gctgctcata
    25081 acaaattgaa ttggtatcat tccctgccgt tgtaatcaaa aagaatagcg gttgcgttct
    25141 agcatcccct gcccctttcg tcatgacatc aaacagcttt ctgttaggtt gtgcgtgaag
    25201 ctcatcaaat acaaccgcag aactgtttaa tccgtgttta gagtatgctt cagctgataa
    25261 tacttgatag aaactattcg ttggctgata aataattcgt ttttgtgaag tcaggatttt
    25321 cactcgtttg cttaacgctg ggttctggcg aatcatatca acagctacgt caaatacgat
    25381 tgaagcctgc gctcgatcag cagcacagcc atatacctca gcgcgttctt ccccatcacc
    25441 gcaagtcaaa agcaaagcca cggcagccgc taactcactc ttaccattct tcttaggtat
    25501 ttcaacataa gccgtattga attgtctgta gccatttgcc ttaatcgttc cgaataagtc
    25561 acggataatc ttttcttgcc aaggcatcag tttgaacggt ttcccgaacc actgaccttt
    25621 cgtgtgtttc aacaactcaa taaaggccac ggcataatct gcacgtgact tatcataatg
    25681 ggagtctttg tccataaatt cagttggttt gtaattatcg attggaactc actcccttct
    25741 acgcaaaaag cagctgttgt cagctgcctt aatctcttta ttccatcata tcggcaatga
    25801 actgaatggc ttcagggttg ttcttgttat cccgcagcaa ttgcaccagt tcttgccaat
    25861 cgtagtatcc ttcttcaatg ccatcttcat tatctggcat gtaaatttga taatccatca
    25921 tttatcaccc tccaagataa agcggctgta ctcatctttg tgttcttcaa tgaaaatcac
    25981 caactcgtaa aagtgccggt catgtgcaat tcgctgcact gcaatcgcat caaacatatt
    26041 tgtcagacca ctttctgcaa tctcaatgat ttgttgctta atctttgcgt tcattttgac
    26101 cgtcctttct gcctgcctca taggccgcaa ttaaggcatc ttccaaacac caaaccgcaa
    26161 tgtccaagaa gtcttcacgg tcgctgttgt gggccttcag gtcgccacgt tcaggcaact
    26221 gaacgatgta attctttgcg atgttgtaaa tcttacgttt actcactttc atcttaaagc
    26281 ccctttctga tgtagatggt atcttctgtt tttgactcaa ggtcaaccca gaccaaatcg
    26341 tggttcaata tcatgtccgt cacgtattgc caagcatcat tgaaggtttc aaatccttct
    26401 cttctaccgt tttgaaagac caattcgaag tctggtttga tgatgtgcac cacatcttcg
    26461 ccgtataccg cgttcagtgt tgaaccgttg tcccattgga taaggattga accaatgtcg
    26521 tcaacaccgg ttactgtgcc ttgtgtgccc ttcgctgggg catgtgggtc gttcatcttg
    26581 atgagttcta cacgactacc ttcagggtaa cgttttttca taccctcgat ttgttcatga
    26641 gttggaaaca taactttgtc ttccgtattc ttttggtagt ctatatatca ctctaaaggg
    26701 attttatagc aagtgatacg ttatttattc aggattttct aggtcattaa atgcgatagt
    26761 ctttccatct cgaatcactg aaacgtctgc atcgcttcca gcctgctcga tataacgctt
    26821 aacaatcaca tcacagaact tttcatccag ttcgatcgta tggcaaatcc ggtcggtctg
    26881 ttcgcaagca atcaaagttg atccgctgcc accaaaacta tccaacacga tgcagttgct
    26941 gagtgacgaa ttggtaatcg gatatgccaa cagcggaatt ggtttcatcg ttgggtggtc
    27001 accattcttc tttggtttat caaattccca gatggtcgat tccttacgtc cggtgtacca
    27061 ttgatgtttg cctttcttct tccagccata gagcaccggc tcatgctgcc attggtaagg
    27121 cgaacgacca agcactagac tctgtttctt ccagatgcac gtacctgata aatagaaccc
    27181 tgcatccacg aatgcttttc tgaagttcaa gccttccgta tccgcatgga aaacataaat
    27241 actggcgtcg ttggccatca cactttccat acaagaaaag gcatcataca agaagttgta
    27301 aaatgcctcg tccttcagat tatcattttt aatcgttccc gctttacctt gatatgagac
    27361 cccataaggt ggatctgtca ccacaagatt tgctagtttg ccgtccatga gtttctcata
    27421 agtttcaggt tttgttgagt caccacaaac taaacggtga cgaccaagca accacaagtc
    27481 ccctgtttta ctgaaggctg gcttttcaag ttcctcatct acatcaaagt catcatcttg
    27541 ggcatcatca ttaccgttga gtaatttatc aagttcatca gtttcaaaac caagcaaatc
    27601 caaatcaaac tcttcacctt gcaggtctga taactcaacc gccaacattt catcatccca
    27661 accggcgttc attgctagtc ggttatcagc aagtatgtac gcacgctttt gtgcctcggt
    27721 taggtgttcc acaaacacgc acggtacctg cttgatacct tcttctttag ccgcaagaat
    27781 acgtccgtga cctgcaatca agttataatc cttatcaaca atcaccggat ttatgaaccc
    27841 aaactcacgc atagaagctt gaatctgttt cacttgatcc ttactgtggg tacgggcatt
    27901 tctggcataa gggaccagct tatcaatatc aactttctca aaacgttctg ttgtgttcat
    27961 tagaacaacc cccattctgc tagtttctca aacccgccaa gctcattcac ataatcacgc
    28021 gcaacttcca cgatttcaga ataaggcacg ccatcaatct cagtgtcacc aatcgcacag
    28081 cttaactcaa cgggcatacc agtttcttga gctttcaaga atgcgtagat gttcactgac
    28141 acatcagctt ttgataagtc tttcccatgg ataccgccac ctgttactcc gtctgccata
    28201 tcggaaccaa gcttgcgatt agtcgcacct gtatccacat ccgggccgcc agtccaatca
    28261 cctagagggt tgactgtagc gtttggatat ttttgtttta actcatttgt ctttgcgtgg
    28321 ctctgacaaa ttatcaagcg ttcaccatca agaatgtact ttccatcaaa tggatggtcc
    28381 tgatataagt cacgcgcaat ttttgagagc tgtaactgtt catcagtcaa tggcatccca
    28441 cgaaagattc cattatcgcc acaacgaatt tcagtcgctt gattgtcagc caactcttta
    28501 tcttggggca caatcaccac atctttttta attttgcctg cgatacgatt aatcgcttgg
    28561 ctgatttcat tcaaatttaa agcagttgaa gtttcaataa tcacatgaca gatgccatga
    28621 ccaattaaaa cctcaactgc tatctttgga ttattttctt ctgcgtatgc caagtccaca
    28681 atggctccgg caatacgatc tgcaacttta tctggatgtt gcggattaac cttctctatc
    28741 ataaattctg ttcctttcaa ccattcttag aacgtaacaa tcgttccatc gcatccgtat
    28801 tcggatctcc tgtgtattcc actgatgcat tctcttttac aatctgttga atctcatacc
    28861 acgtcacatt tgcttgcttt tggaagttct gcgacatcgc cacaaatggg gaggccatga
    28921 ctgccttggt agtcgggtgt tttccgagca aaccatattg actcagagca ttttcacatt
    28981 gaacaaaacg cgcaaagttc tgagcgtagg actcaaccat tcgtggggct atcaacgttt
    29041 cgcatccgcg gtctttcaac cattggaacg ttgagttata aatttcatca gcgcctagca
    29101 cagtgccatc cttctgtttt gcagaaaggt aagcacttgg ttcaggataa tcattgccct
    29161 caagctctac accctcacca atatcttcac caattaataa ttcaacatcc ggtaagtcaa
    29221 attccatccg cgatgctttc tttccagcag caactttatc aacgagtggc tgtgagcgtt
    29281 cccctgaacg aggtcgtctg ccaccgcgtt gtgttccatc ttttgccaaa atttatcacc
    29341 tctttttctt gtttggggct atcccccgtt tgaactgaac ttttttcgcg taagggccta
    29401 gcgcgctgta cagtttagaa gggtttaggg ataattaccg cccctccccc ccttgttatt
    29461 tttcacatga ttgttttatc ttttgtgttt cgtcgagtca tgtgaatttt cgagtgacat
    29521 gatttgcaga cactcatcaa attattttca tcatgcgtac caccatcact taattccttg
    29581 atgtgatgca caatctcaac cgaagtaaag cgtccttcct tcagacacat ctcacatagg
    29641 ggatgtgctg ccacgtacct tgcccgtatt ttcttccacg ctctgccgta acgtttgttg
    29701 attgccggat cacgctcgta tttctcgtaa cgtttcctcg caagtgtttt atgttcatca
    29761 caataagtat catgcgtacg gttcggacaa cctgggaact tacaggggtt agctggtttc
    29821 attggcaagt ggggtcacat cctttcgggc aaaagaaaag cccttgaagg atttttccca
    29881 caaaggcttc gagtatttgt tcgatacttc tattgctggt attgcttgag tatcgtttgt
    29941 cttttttgac atcttaatga tatcactaaa ggctactgac attcactgac acatttcaaa
    30001 tgactatggg ttcatctggt agcttcaagt tctcaagagc tttgctatgc caacgaaaga
    30061 ctgtccgcac gtcagcgtga agatcttcag caatcttgtc ccagctctct tccaacaggt
    30121 atcgattgat taacacaatc ttttcgtcca cgtttgtgag ttcatcaatc acgccacgca
    30181 tttgtcgttt gaggtctaca aacttatcaa tctctgcatc aatttcagct tccaattggt
    30241 aacacttctc aaggttacga acaaatggtg cctcagtatt ccgtgtgcca cttgaaatct
    30301 tttcttcgaa tgttgtggaa gaaactgtca ccgccagttg atagagctcc gccttctctt
    30361 taatccggtc gtcaattcgt ttattcaaac ggtacgcttg tttcaagtat tcttttggtg
    30421 tcacttgctt ccctccaatc tagctttcac tgcatcaatc aaacatgatt gtgttttatc
    30481 ttttcgtttc aaagccaaca tcacatcttc atcaatcgta tctttcgcaa taatgtgatg
    30541 aatgaccaca gtatcacttt gcccttgtcg ccacagcctt gcattggttt gttggtaaag
    30601 ttccaaactc cacgtaagac caaaccatac aagtgttgaa ccaccagctt gtaaattcaa
    30661 tccatgtcct gcactagccg gatgaatgac agcgatgggg atgctacctt cgttccaatc
    30721 aataatgtcc tgagacgttt tgatctgtcg aacgttgaat cgttccttga tacgctccag
    30781 atcatgctgg aaccaatagg caatcagaat cggtttgcca tttgtacctt caatcaaatc
    30841 ttccaaagca tctaacttct ggtcatggat gtgatgactc acattaaact catcatagat
    30901 tgctccattc gccatttgta gcagtttatt cgaaagcacc gctgtattgg ctgcatcaat
    30961 ggtggcttct ttcaattgta gcaccaagtc tgccttaatt tcatcgtact tttgcttttc
    31021 tttgagtccc atatcaactg gcacttcatt catcacaagc tctggcatat ccaagaaatc
    31081 gactgacttc attgaaatcg tgatatctga aatcttgtcg tagatttctt gttctgcatc
    31141 ttcccttggt ttccaagaat aaatctgcat gccattttgc ttgtcaggta gaaagtattt
    31201 atctcgataa cgtgaaatat aatagcccaa tctctcgccc atatccagaa ccttgaactc
    31261 cgcaaacaaa tccatcaaac cattacttga aggtgttccg gtcaatccaa caattcggtc
    31321 aaccttgtgt ctgactttca taaagctagt gaaccgttta gcttttaagt ttttgaagga
    31381 cgacagttcg tcaatcacca ccatatcaaa atcaaacgta aaacccgatt tgttaatcag
    31441 ccagtccaag ttctcacgat tgatcagata gatgttggca ttattttgta atgctgccat
    31501 tcgctgactt ggtgttccaa gaaccacgga atacgataaa tctttcaaat gatcccactt
    31561 ttgaatttca gttggccatg ttgattgcgt cacgcgcaac ggtgcaataa ttaagacacg
    31621 ttgtattgta aagtcatcat gcatcaagtc tttaatagct gtcagcgttg tcaccgtttt
    31681 acccaagccc atgtccaata gaagggcggc agttttatga tctttgataa aatcaatcgc
    31741 atatttctgg tagttatgtg gtacgaactt catcagacat cacctcctat tctttctagt
    31801 aactcaggta tctgatcaat ctcatcaagc acgaaacact gaaaccctaa atctgtcagt
    31861 tgtttcatac gaaaaagctg taaagaccgt ggcttcttcc ctggcgcctt cacttcgaca
    31921 aatccaagtt tcccatttgg cataagtacc aatcggtcag gcacaccagc aagccccggt
    31981 gatgtaaact tcgggcaaat accacctacc cttttgacgg ctttcactag tgcttgctca
    32041 acctgctttt ctctcatagc cacaccgtca aatactcgaa atacgcatca cgcaagattt
    32101 ccaagtattc cttttctgcg cagtgaatca tcagatgtgc gacttgctta tcgaatgatt
    32161 tcttccaagg atacgtgaca tccgacatgg caaactcgta aaactcatca aatcgtaaat
    32221 cacgtttacc gattccaatt tgtaaccact gtttaaattt catttagata ttctccaatc
    32281 tgcaagtatg gtgtaacccg tgaatacgca atacataact ttatataggt ggtaattttt
    32341 tatcccttaa gaaaagttct atataagacc tacacaggtt acactttcct ttaaaatcaa
    32401 ctattccaag aactcggatt tgagttgcaa accaacaatc ttgtttcctt tattagttcg
    32461 ccggcgctta aatccgcccg cctccagagc cgtgtaaaaa tcggctgaac tacgaatgta
    32521 ctcaccagtt cgcgtacaaa acgcacgata ctctgcatac aactcaccag atttttcttc
    32581 aaagtcaaca gcagtctcgc agcactcgtc taagaaatgg ccaagccaat cattcgccgt
    32641 cttatattca gaaattgcat tagcgacaac ttgtggaacc gttaaatgaa agtctgcggc
    32701 gatgactttt ttcgcaccgt caaggatcca ttgcaaaatg gctccgccgg cattttcaaa
    32761 aaggtagtcc gcataattct tgatgtcctt attgccgtca atcgtagcaa ggaatggaat
    32821 cacaattaaa cgacgccagg ttcctttatc aatcgctcca acttttggga ggtggttggt
    32881 atagagcacc aaagtatgcg ttgggacata cttgaatggg tctttgtatt tcttctcagc
    32941 cgcaatttca tccgtcgagc agagctgctt aatgttagat gtgttgagtc gcatcccctc
    33001 ttcaagctcg gctgcgatga gtaggcgttt ccccttcgct tccgcaagtt caggtttcac
    33061 attgcgtcgg atttgacttg taagaatatc agctgagatg ctgccagagt aattaccgag
    33121 cacacggctg atgacattcc agaacgtgga cttcccgtta cgcccttcac catacgagat
    33181 gataagcgct tcaacgtaaa ctttaccaat agctgcaagt cctacaatca tctggacata
    33241 atcaatcaac tcttggtcac ctacgaaaat ggtgttcaga gcgtccaacc aaatctgctg
    33301 attggcgtca ctcggggcac attccgtttg cttcgtgatg taatcttctg agttgtggtc
    33361 ttgtgatttg cctgttcgca aatcaaaggt tgctgttggt gtgttcagca agaactcgtt
    33421 cacatctagt aaccgttgct cgatttcaag catgggcttt gcctcggtta atgctgacga
    33481 gagatacttg ctgtcgcggc gtttgacagc aaacttctta tactcttgtg cgtgttggta
    33541 caaatcaaaa ctatgacgtt gcgctgtgtt gaacatggtg actgcctttt tcggaccaac
    33601 actagcaagt acatcgaacg cgccattact aaccatttcc ttggtacgct tctcaatttc
    33661 ggtttcagat tcttccagtt gacgttctgt caatgcgtgg atcacacctt gcgcttttgg
    33721 tttcgactct tcccaatagc tgccgttgta gacaaggtaa ccagttgacg gtgagtaatg
    33781 aagcttacat tcatactcgc gagctaagac cgttgcttga cctaagtccg agtagtcggt
    33841 tggctcaagt gataaatcag agttgtactg ctctggagga atgtagcctt cttgttgttt
    33901 cactttcttc ccaaagttga ccgcgctatt ccagatagct ttaagctcct cctccggcag
    33961 tggcggatta catttttggg attgttcgag atacttgtga tacgtatctt ccgttgcacc
    34021 gtagcgtttg atgagctttc ctgcgatgtg actcatgctt gaattacggc tgccttcgct
    34081 aatctctgac tgcgcattat cccaatcagc aaacagatca tcttcgagaa aatcagtgat
    34141 caacttacta ccttcataga tttcaacttc agagtttggt gttccgaaaa gcaaacgtgc
    34201 cgcatccatc gcattgttgt caaagtatgg gaactcactt tgaatctgct tcttgagttc
    34261 cacatattca tctttatcag ttgtggcagg aatcgggaaa tatacgtgaa aacgtggacg
    34321 tgctgatttg ccacctttgt ctttcatgtt gttacgtgaa tagctgaccg caaacgtcac
    34381 atcaggaaac tgcatcgcca cttcgaaagg ggctacccaa tcatctggat tgtctgagtg
    34441 gtcattgtcg caatcaagtg gaatcacatc agactggatg aagttgtcac ctttgcgata
    34501 gttgcctttg tactcagcca tcacatgatc cagcttgacc gtggcaacaa gtgtttcttt
    34561 atctgtcacc acttgcttgg ttgggaacaa tccattcttg gaattaccca cctgattgga
    34621 tgtgaataac gtcatcttaa gcatttgaga cctccttcat ctcatcatcg aagtacctga
    34681 tatgtttctt ccaatactcc gcttgttcaa tttcaactgc catgccttgg gagatggtgt
    34741 cacccaccac ccagacttcc tcacatttgc ccatgagaac aagtgacatg tgcattgcca
    34801 catcacgctc cgttgcgtcg tccatgaatt gtggatacat cagatgtgcc gcaactggca
    34861 tgtagccacg gtcaagagca atacggcagt aacgcttagt gtttttatgg ttgacgacaa
    34921 catttcctga gaacggcgag cagacataga ctgatgttct gtaatcttta cgtctaggca
    34981 ttattatcac ctcgctcaat cactggtaag atgttcttct tcgatttcag caagtcgtac
    35041 aagaacaaac gacctttctg cgtccagtag gtatgcatca cgctgcgctc tgcatctatc
    35101 gcatgagttt ttgattgtgt gtagccttga ttggcatact tctgataaag caaccacatc
    35161 ttgccttgct tgtactgaat tcccagttca tgaagcaact tgttcattgc ctgtggtgac
    35221 ataccgtagt ccttcgagat tttagaaatc gacatcacag aattgttttg aagaatcaaa
    35281 tcgtagtagc taacttttgg tttcatctcc aaaatcattt gttgttgcat cgccaccatt
    35341 tcttttttct cttgtagctg cttcaacact gaaatggccc atgctggatc ttcgattgac
    35401 tttgtgatga aatcttctgt cgcgtacaca ccgtgttggc ggatagacgg caagacctca
    35461 tttgtcaccc aacgcttgaa tttcttcgcg ttcggcatct tgctagaaat gatgagtgag
    35521 tacaatccac tttcgttgat aaggatagcc attgtcccat taacgctgaa cgattcgttc
    35581 accgtcttat cttcctcatc aacatgatct cgtaatgctt tttgtggatt tgtgtagcca
    35641 agaattgatg ctacatcttt accaacaaag tgagggacat cgccaatcat caccgtgcgt
    35701 acagagccaa actccgcatt ttcaaaaact ttcaattcgt tcatcttgtg aacctcctgt
    35761 aatagaattt tcaggagagg cattttgccc cttctaactt acaggcgaag aaaatggtcg
    35821 aaagtaaact ctgactcgaa tttatttttg atttttttgc cttcgtctat aagcgaagaa
    35881 agaacccaat tcgaacctta tttttgataa aaaaatttga aaaaaatatg gagccatttc
    35941 tgactccaca ttgactaatc cttttgatag aattcacact cgtatccatc agcgttcagt
    36001 agcaagcctt cagcccactt aggcgtttga ctcatcaact gacagatgtc ttcaaccttc
    36061 gattccattg gcacttcgat aatcacttcg tcgtggacgt gcccgacaat gcgatactta
    36121 cgaagattct ccattgcgaa acagagaatg tctctagcta ttgcctgcac gatgttttcg
    36181 acaaacttgg gaccatagct ttcaagacgt tcccatttct tggtagcacc gacaccttca
    36241 taggtaacag cttcaccgcc gaatttgttc tcaccaatct ttggttttac ataaactaat
    36301 ttacgtcctg acaagagttg gatgaacaga aaacccgact tgtattcaaa cttgatgcca
    36361 tgcgtcgctg tgctccgtcg ttctttcaca caagtcttta ctgccttatc aacagcccac
    36421 caaagctccg ttatgttaat gtttgagtct cgccaagaat taacaagcgg ctgtaattca
    36481 tcctcagtta gtcccatttc aagagcatcc attgctttca atgcacccac cgatcctccg
    36541 taccctagag caagctcagc aattttccct ttttgacgta gatgcccatt cacgccatgc
    36601 ttttcaactg gcacaccgaa catctgactg gcagacgcac agtaaatatc gccaccattt
    36661 ttgaatactt cttgacgcca cttctcaccc gacagccaag caatcacacg tgcttcaatc
    36721 gctgagaagt cagatacgat aaatttcatc ccttcacgtg gtacaaacgc tgtccggagg
    36781 agttctgaga gtacgttcgg gacattgccg tagagaatgt taagcatttc aaaattgctt
    36841 tgttttacta aactacgcgc ttcagataaa tctgacatat ggttctgagg caagttttga
    36901 agctggatgc cacgtcctgc aaagcgtccg gtacggttgg ctccatagaa ttggaataaa
    36961 ccacgagaac gattatcaga acacgcaaag ttttgcatgg cttgatactt tttcacggat
    37021 gacttggcga gttgctgacg taagaccagg acttgcttta aattttcatc aataggttgt
    37081 tcgagtagtt cggccactgc ctttttaccg agtgactcgg tttcaagtcc gtggtctagt
    37141 aaccatcccc gcatttgaag cacgctgttt ggattttcta gtccagttaa ttcttgcaat
    37201 tgttgattca atccagcttt tgataattca tccaactgaa cggcagcttc cacaaagtcc
    37261 ttatcaatct caattcctcg gtcgttgatt tcctgatcca gccagtattc tttccaaaca
    37321 gaatcaagca ctggaaaatt ggaaagtttt ccttggatag ctatttcaac ttccacatca
    37381 cgactattgt atgtcttaaa tgctgaccat ttagttggcg catggtgtgt aagttcctcg
    37441 tgcgtccacc attaactttg gtcggattgc atggcacgca aaagtatcgg atgaggtctt
    37501 ttccttctgt gagcttttgt ttatccaaac caagaacagc accaactcct acaagtgaca
    37561 acggcaaacc taatgtcgca ccccacacca ttgtgcattg ccaccctcgt ggattcaaaa
    37621 agccatccaa ccccagaaag cgtgacaaac acacacgttc aaaactcgca ttgaaggcgt
    37681 gtttaatcac tgtttcatct gatagcgaag ttaatatttc agccgggata gtttcgtcca
    37741 tcttcagatc aaccacttcg acgggaccac catctacact gtatgcaaat agcagtattt
    37801 caaaatcggg ggactctgaa tatttgtaga caccacattt cgataaatcg acactactat
    37861 acgtttccaa atccaatgac aaatgtttca ttttcttcct ccaatcaaac aggcgacagc
    37921 acttctgcca tggccgtttt tgttttaatg attaagccaa gaaatcatca tccgccaagg
    37981 tttcaaactc atcgcttgca gatacttgtg agccgagtgg ttcgccgtca cgtacaaact
    38041 ggatattccc aagccctgcc gctaccccgc gatttccatt tgcgttaaag gcatagaaat
    38101 taatcgacac acgagcgtag caaccactat acacttgtga acgatccaag attggttgaa
    38161 cgtgctgatc cacgatttgt ggtgcagtgg ttgagttcgc attaatgaag taatggcctt
    38221 ggtatgcttc atcgtcgcgt tcgatatccc catcacgtaa tggcagcttg atttctgctt
    38281 tgtttggttt cttcccaccg aatttcgcaa gtccctcttc gattgctgca tcgattgctt
    38341 tttcaatggt atcaatcgtt ctcttatctg attttggaat caagactgct gttgaatatt
    38401 tctctgcgcc cccgttgatg gatgctggtt cccaaccgtg gaagtatgag agacgtgtgt
    38461 ttgtaccagt gattactttg cttgttttag acatattatt gttcctccgt taatggttta
    38521 aattcttcgt ttaagttgtt tactgtgacc gcttgtcgtt tatctgaacc atcgactagc
    38581 gtcagcttgc cttttggttt gatgatgtat tcgcccaaga tttcattgaa tttctcttta
    38641 ccgagtgcct tcgtcattgc tgtgacacca agtaactttt tatcatatgg gtcgtatcca
    38701 gccttgatga ccgccgcagc gacatcattg tcattagaat acttacgagt agagcgaccc
    38761 tcgactaatt tgtgatgagt ccattgtttg ccaccgagtg caagtttcaa agcacagtcc
    38821 ttgatatcgt tactccaaga aaccaggtca tccactttac cgatgatttc ttcgatttca
    38881 tcatcactaa gaagctctgg tggtttgaac tcatattgcg ccagtttcaa gttatggtct
    38941 gctcgtgcac gacagctatt cttcgccgga cagaatccac accatttccc gcattggtac
    39001 tcaccctcac ctttgaaagc taaatctgcg gcaggcgaca acacatcttt tgcccactga
    39061 agtaattctt ctttcagcag tgtgtacgta ctcacattct cacgccttgg ctggaaaatg
    39121 gtcatcttga cttcttgaat gtcataaata acatcgaaaa gctccaacgc tcccaaggca
    39181 taacacatca tctgtgggtt ctgatacgca tctaccaaaa ttcctagacc gtatttcatg
    39241 tcaataacag atagggttcc atccgcaatg acgagacagt cgccggtccc aaagccgtct
    39301 gggacgtacc ttgagaagtc gagccgctgt tctactatta caatagagtc aactgttgtc
    39361 tgtttcgcct tagcgacttc ttccatgacg aaggtcgcat aactttcagc acactgctcc
    39421 atctcctcgt cgtagaattc aagcgtagat gttgggtctg tgacgtcatt accaatgaac
    39481 ttattcagtt tgtactcaca taaggtatga gcatcagtgc cttgttgggc atacgggctc
    39541 gacttgtttt caaagtgctc tgacaagcga acacttggcg gacagtgaat ccaccgatta
    39601 cttgaagatg cggataggac tgcgtgttgt gttggcatct acaatccctc cgcatctgca
    39661 accaaagccg cgtagttttc tgtcggcaca tcactcaagc gactggcacc atatttctca
    39721 atcagtgctt tcacttcagt tgacttacct tcttgagatt tacgtgccaa caaaccacgg
    39781 acttcttcaa gcgtcggttg tttgacttct gattttgttt ccattgtcgg ttctggtgtt
    39841 tcaacttgtg gttcaggttc attggttttc atcgccacag ccaactcttg taagctatca
    39901 gctaacgctt ggacgtcctc aacgacctga agaaatagtt ttgttttact cacgtcatta
    39961 cgctcccttc ttgttaactt cacggatttc gactgattcg attgtgtccc ctggtgtgag
    40021 cacgagaatt ttgtccatgt tgcccttacc gaacagccac cgtgcaaacc agcgaggcaa
    40081 atgacgcaca ccaccttgta ggacagtttc tttgttaccc gtgtcatcag tcacgtttac
    40141 tcggattttg tgttttaggt tcatgatggt cctccttcat ctttatttgt ttaggatcta
    40201 ctcccttaca caatacaggc gaaggaaaac ggggaaagta aactcccccg taactttttt
    40261 ctaaaataat tctttgatct tgtctttgat gattccaaag gtgcgattga ttgactgttt
    40321 tttcttgtca agcttgtccg ccacttcagt ctgagacatg ttttcagtga cgagcatgtg
    40381 atagatttct tggtggtacg gtaataattg cgtcacgact tcacgcaaac gatcgatacg
    40441 aggatcttct tcattatctt ggtaagcatc gtaaaccaat gattggatgg ggttgtcccc
    40501 ctcatcgcat tcatcatcaa attcattgag cgacaaattg tagttacgtt tgaagtaatc
    40561 gttggcttca ccatccacgt attcgtccat tggataatag ccgtgctcat tattaaagtc
    40621 ttcacggaac ttctctttcc aagccttgat cttttctttt tcttcttctg tgcgttcagg
    40681 acgtgcgttt ttataaaagt tgtaaatcgc acggtcgtca gcgcgatgca gctcctgaat
    40741 gttaagctca gtgacgccgt cttcgcctgg gcgaatctca accacttctt tttggatacg
    40801 tccattggca tcagtacgct caaacacgta cttgtaagtg ctgcggtcgt tttggtgtgt
    40861 tttcataaat ctactcatta gatccctctt tcttgcctga aagagctttt gagcaaggaa
    40921 aacaaacagg gaccgtgcca atacgtacac cgtccctcat cacgaaaaaa gagcgcaata
    40981 aagtaaggca tacaatatct gaaaaatttt ctggataacc agttcttttc agtatttgta
    41041 accttgccct attgcaactc aggcattgtt atattttatt agtaggacta ttcgctttta
    41101 agataaattc gttcactttc ttgaaacata atgtagtgtt tatgaaccat aaatgataaa
    41161 attaaatagt taaactacta tgttttaatt atagaaaaag gcctcccttg ttttcgtcag
    41221 tcatcggcgg caaacggcgg tcaacggcgg acgtaacaaa ggaggttgta ttttggaaat
    41281 aagtgaaatt actcaaacgc tttatccgtt tatgggaagc gagtacgtga agccacagga
    41341 ttttttaatt gctttactgg atagtgtttc aaatgatgac gaggccacca ttttcggttt
    41401 ttctgatgat aacctacgaa gattttacaa tggtactcga ccattaacac aagagtatgc
    41461 agttcgggtt gctggaaaat tcaacatcga caaatgcacg acttttattt atgaacgtct
    41521 tatagatgat gctccaaccg tccttaagca acaattgatt gataaaggtg tggtaatccc
    41581 caaagataca gacgtttctg aaatagtggc ccatctttta cttgatgaaa tcacctcaat
    41641 tgcttctcaa acaaaagtaa aaacaaaaaa ggccaagccg aagcttagcg aattagagct
    41701 cgcaagttta agaatcaaca ctaatggtga tttctcttat aatgatgaag tgaaaaaatt
    41761 ctttgacgat ttgccagttc cccttgatat tagagctgag gagatgacgt atgtcatagc
    41821 tcttttttca gcatatgcag atgccgatgg atacgaagta tacgagcata caaacgacct
    41881 gccaaattcg cggctaaagg atttcaagag acagcgaaga aattactata aagctgaaag
    41941 catacgtcgt ggggtccgtg ataactttac acaagaggaa ggaactgagc actttgaagc
    42001 tttaaaagaa gatatgtacg acggcatcga ggaagtctat gaagaagact acgatgatgg
    42061 cgttgagaga ctcaaagccg ttctacagca gtcgtctgtt ataacattaa acggatcaat
    42121 cttatcgttt ataccgggat tgctgcaaaa ttctgctaaa aaaggcattt gccacatgct
    42181 ggttaatgac ggaaagatca aatgggtgac tgaagatgaa tagtttgttt aatactccat
    42241 ttgaaatttc ggttcgagta atgttactgc tctctgaatt taaagagcct gtcagcgctg
    42301 atagaatttt aatcacagac ttcatcacca cgtatggtcg cgactttgaa atttccgatt
    42361 acaacttaaa tggtgataac agctatagat tcagtgaatt tattgcgcga cgtgaattag
    42421 ttaaagatgc agtcaaaaat cttgtactgc aacatgttat ccaaccatcg caaagcaaag
    42481 aaggttttaa gtatgcttta tcagaagatg gcctaaagtt gatatcacaa ttcgtatcgg
    42541 tatatgcaga aaaatactta caaatagctg acgaatctgt taaatacatt caagataaaa
    42601 gtgacgttga gcttctgcga caaataaacc aacgcgcaaa ggaatttaaa ggagagcaat
    42661 gataatgcaa aattattata ttgaacgtgt taccataaaa ggcaaagatc gtgaagatgc
    42721 aactgtcaag ttcaccaatg gactaaacat aatttctggc ccttcaaata ccggaaaaac
    42781 tagcattgtt acggcaatcg attttctcta tggcgcgaaa aaagacgctg ctcccttttc
    42841 aaccgatgca accggataca gtaaagttga actgattatt caatcgacta atggcaaata
    42901 ttcattaact agagaatttg atagcaactc cattttggta tccaaaataa cgagttcagg
    42961 tgaatatgta gattatgaaa aatataatgc acgatctggg aaaaatccaa ttagccattt
    43021 ttggttacaa cttttaggca tcgatgatga ttatcagatt atcaagaatg aggatttcaa
    43081 acgtcaaaaa ttaacttggc ggacattagc gcctagcaca cttattccac aagatagagt
    43141 caataccagc gcctctgttc tttttcctgg ggaaacaacc caacgcacag cttttttatc
    43201 atcgttgtta cttctgctat acggttcaga ttttgcgaac tcggaagaaa acgaaacaaa
    43261 agaacataaa aaaattagaa ggaactcggt gattaactac atcaattcaa gcctttctga
    43321 attatatgaa aactacgaag tggctaaatc agaattacct gaggctccct ctgatgattt
    43381 tcaacaaatt gtcgatggaa tgttcgctga acttgaaaca attgaaaagc aaatcaatga
    43441 tgcgttagcc agcagcaaaa tcttgataaa agagatttat caaaaggaac agtatcttca
    43501 tgaaactcaa cttcttttga ataggtacaa acagttacat tatcaatatc aatcggatat
    43561 tgaacgacta acactaatcg tagatggtga gcgattatta aatgatcata atgaacatga
    43621 tacttcacat agctgtccat tctgtaacgg cgaactaagt aaacctgtcc aaacaaccta
    43681 catcgatact gctaggtcag aattacattc attgctctct caattatcag atttgacgga
    43741 agaatctcat gataccgaag ttgaactgca agagttaatg gaacagcttg atgagcttaa
    43801 aacaagaaag aaatcgcttg atgcagcaat caacgatgaa ctaaaaccga aagctgagac
    43861 gttaaaggat cgaatcaata aaatcgaaac ttacagggat attaaacaac agctaatctc
    43921 gatggaaaaa atgcaaaata agcttaaaag tgatcttgaa aaaatcgaga atgagccaga
    43981 agacgaacgt atcctttata aacctaaaga gaaatttgat gcagaattct acaaagaaat
    44041 gtctgagtta ctggactcca tgttacgtaa tatgcattat aaacctaatg aatttcgtaa
    44101 cgcatttttc gccaaggata ttttcgacat caaaatcaat ggaaatctaa aatggaccga
    44161 aaacggacat ggatatacag cgtttatcaa ttcagtagtg gtaatggcat atcgacatct
    44221 cttgtataat cacgctaagt acacaccttc tgtgttcatt atcgattccc ctcttcttgg
    44281 attagatgaa cgcaaaaaac aagatggcga tgacaatatg aaagaaggac ttttcaaata
    44341 ctttatggat catcaaaacg aaggacaact tatcatcgtt gagaatacaa cgaacttgcc
    44401 agcactaaac tatgatgata aacaagtaaa tcagattgtc tttactgaag ataaaaacaa
    44461 tggacgctat ggatatctat acgatattta taactagaac aggaatgatt acatgaagtt
    44521 tagctacaat ccgctttgga aactcctaat cgacagagga ctgaataaag cacaactcag
    44581 aaaaatgact gatatcagtt cagcaacact ctcaaaacta aataaaggcg aaaatgtaac
    44641 aacagatatc ttacttcgca tctgtgaagc acttgattgt gatttatcgg atattgttga
    44701 aatcgttcgc gattaaatat caaaaatgca ctgtattcga ttcatgagta cagtgcattt
    44761 tcatatttat ttttgtaatc cagcgattac ttcattgatt ttcgattcaa tatcattcaa
    44821 tggaaccata taccattcag tagctttttt gaagtttcca tcctgaaaga caacttgttt
    44881 gtcctggaat acatgatgga gagtgcgctc aagtagctga gcgttgacat tttgtacttc
    44941 agcagaaagc atgaccttca ctggtgcatt taagtaggtc acgtcgtttt ccgcattggc
    45001 aattctactt tcaacatcat ttcgagtgaa accaatctta tacaggttat tccacttgct
    45061 aatatccgga ttcgtgctta atgacttcag cacgtagata taacctgtcg taaactcttc
    45121 tccaaagctt tcagccagtg tttcctcatc taccggctct gtcaccatcc ttccatgacg
    45181 ttcagtcgag tatagagacg aagctaaaga ttgtaacaac agattcttat tctctgtccc
    45241 attctcataa acaacatgca ttctggcatt tgttttccca ttatcagcga taaaggtatc
    45301 cttcgaaatg gaaacgatat acatcattac gccgttttgg atgtagaatc tattttcatg
    45361 aatatcatac tgctcaaacg ggataatttt tcgtctgcca gatgcgatat ctgcatgcac
    45421 ttgatcgaac aagctcttat acttttcaaa atgacttgca tgttttctgc ggccaatttt
    45481 atctgcagcg ttcacagttc tacgatagcg ggaaacatcc aacaagtctg aaaaatcatc
    45541 aatcatatca tctgagccta agatatcatc taaattcaac tcgtcatcga tttcaactga
    45601 atcagcgaga aactctccat ccggttcgaa ttctttctcc aataaaccaa gagtgtcata
    45661 cttcttaacc ttctcagacc tttcgggttt tcctcgaaat ccgctaagac gagcccacaa
    45721 tgcgcgttca ctagaccatt caggagtttt ttctggctct cgtccgtttt cattcacaaa
    45781 gtctataatc tcctgaaatt tagcaacttc tgggtcttgt tttacaactt tctttttttc
    45841 aacttgcttg gttaaatcat caaaagcgtc atcttcaaag atgctatcta aactattcgc
    45901 catcttcttt cgctgcctct ctttcacgtt ttatctcagc aagtcgtagc aaagcaaagg
    45961 ccaagtattt ttcaccatca tctttactat ttcgatcagg atttcgacca ttttctgcac
    46021 ggaacttctt agcagcttgg taggccgcaa ccaattgttc gtcagtaaaa tcatacttat
    46081 ttgatgacaa ataatcctga ataaaccgaa gagtcggtgc atcaatatca cgactaatca
    46141 cttcatacgc acgttggaac ggattcactt gatcgattaa atcaatattc aattcatcaa
    46201 tgttaatgaa acgatcggcc atcttaataa tttcgcggct accatcttct gaagttgttg
    46261 tccgtccagc cgcaatattt atatcggcaa ctgtatgttg acgaacggtt tctaactctt
    46321 cgttcgtcaa atcaggataa cgatcaatga tgacacgtgg aatcaacgtc ttattaatca
    46381 tcttcgcatc agcacctgaa acaatcgcat tttgaatatc ttggctctgt aaaatgctag
    46441 atttcaaatc ttccatatca ttttcgatga ttttctttgt acgttccgtc gtatcagcag
    46501 gtttcagtcc tctgattgaa agatcacctg gtttgtctgg cttaccatct tttggtttaa
    46561 acttgtaaac tggcgccaac acttgttcca taagtagaga tgcagaaatt gccttcataa
    46621 tactgttaac cgcgaataat acttcgtcat cctgtgcatc tggctgcgca ataagatttg
    46681 taaactgtgc atgagtctta ttggggctgt cacgagttac gcgaccaatg atttgaatca
    46741 cttcagttaa tgagttacga taaccgattg tcagtgcata ttccgcaaat ggccaatcga
    46801 agccttcttt tgccattcca agtgcaatga tgatatcaag cttatcaaga tctttaatgt
    46861 catccgtaat acttgctaga tatgctgaga ctttttcacg tccttcttca ttcacaaggt
    46921 cggccactaa tagtttttta ccatctggac gttcgatttc ataaatacca tcctcgttct
    46981 gtgtgatatt accaccgtcc gtcaataaat caagtatttc atcaacttcc gcgtatttat
    47041 ctttagtaga ctcaccagaa ttaacattag gaatatggat aattgatttt ttgttgacat
    47101 caattacttt atccaaagca tctgtatatt ttccacgata gaaactatac cccattgcaa
    47161 aggatttcag atatttatat ccttccagtt gctcataata agtgtaagtg accttatcaa
    47221 atttatcttc gtcggctggt tccagaatct gaacagagtc gccacggaaa tatgaaccag
    47281 tcattgctaa gatatgcgca ttagagtttg acatgatgct acgtaaggca ttacctaaca
    47341 cagatttatc atcacggctc acatgatgga actcatcaat cgctaatagc gtattgtcaa
    47401 ataatgaatc gtccaactct tcaaaagcaa atcgcaacgt agcatgtgtg gcaatcagga
    47461 tattatcact tgggtcagtc gagttcatga atttaacaaa gtgttgaacc tttgattttt
    47521 caccgccccc aatagttaag ttgtcacttg gctttacttc ccaatcccaa taaaaaccaa
    47581 acttcgataa ttcagtatct ttaaatgatt taccaattga gcgttcagga actgcaataa
    47641 ccactttttt aattccttga ttatggagtt tatccaagcc aacaaacatc aatgcacgtg
    47701 attttcctga agctggtggt gattttacca acaaatactg tgacgcacgc ttttcataaa
    47761 cacgttcttg catttcacgc ataccgaggt catttgttgt ttttgattga tttttatcaa
    47821 aattaatttc aaataaatta ctagtcattg attgtcatct cctgatagag ttttagtaat
    47881 gttccaagtc gatcttcgtc agattcaaac gggcgttgct gataagctct atcaacaatc
    47941 ccatccagtt cctggtgttt cttacgtaat gattctggca tactgtcttt attatacaaa
    48001 tcggctaacg tgccgccttc gtattctcgt aaatcaagaa tttctgtcat cacgcgagtc
    48061 atttcatttt tacgctgggc ggataaatcc tgaatcggaa aagtattata gactaacccg
    48121 gctgaataac ggtaccgagt ttctaatttc ccaccgacag agcgcagcca caccatatgc
    48181 attcgggatt ctaacagacc aagcagccac attggagcgt tatagattgc catagccgaa
    48241 tctgaaatta tagtatcgct cccaactata cccataggaa cataatctct tttttctgat
    48301 gacacacgag gaattagaat actattctca ctggtttcat cataacgaac ttcaccaaat
    48361 gaccatggct tctcagctag tgctcgagtt tcttttctct tgcttttttc cctataactt
    48421 tttacatttt ttattgtttc tttaataaga gaattttgtt tgtatctagt tgcatcttcc
    48481 tcggtcaacc ataaagtata tcttaagtct ccatttatta atcctgttga tcctaaatat
    48541 ttcttaaata aatgtttaat atctggatat ttttgaatca aattccagta ttctgccact
    48601 gaaaaaacta gattaccacc atcatttgcc atactgccaa atttcaaaga gggtaaattt
    48661 gaaatactat ttcttgattc gttgacaaat acattttcac cattagctaa atatgcatta
    48721 atatttgaaa cactcattac tgacccattc tctaaatata acttaggatg aatattcaca
    48781 ttatttgtca taccaataat gacaacagtc acaccggcat tattctttgc attgttgttc
    48841 cacttaaagc tctgatatgc aaatgaaatt cttacctcat cttttaaaag gtacggccaa
    48901 aggatactaa cttgttcacc ttgacaaatt gaattagtag taacaaatgc gaacgagcca
    48961 tgagatgttg caatatattg tttagctttc caaaaccacc caaaaatata atcaagcaat
    49021 ttatattttc gaatatcgcc aacaagtttt tccatatctt cacgttgttc agcattttga
    49081 agacttttgc ccagatacgg tggatttccc attatatata tttcatgatt ttctttgagt
    49141 cgcaacacct cattccaatc aattcgaaga gaattggcac acactatatt cccagcatct
    49201 tttaacggta acaactcagc aatggtacct ggtattgctt cttcaagctc tttattcatt
    49261 tgatgttcag caatccataa tgaaagtctt gctacttcat gcgcaaaatc atccaattca
    49321 atcccattaa aattaccaag atgaatcact gatagtggca tggaatcatt attttgtaag
    49381 tcgcccaaca attgcaaaat cttaatttca agtctacgga tttccttata agcaatgatc
    49441 agaaaattac cagaaccact ggcgggatcc aaaaacttaa tattagaaat tcgcgtcaac
    49501 aaagcactta gatcattgat gattgttctc ttattatcgt ttcttgtttt ttcagtaatg
    49561 tccttcagct cgttttcttc actgcgaagc tttaaagttt caaatgactc tgtcaaatca
    49621 tctaagaata acggcttgat gactttcatg atattcggca cgcttgtata atgcataccg
    49681 gcaacgtgac ggtcttctgc tgaagcaact gattgaatca ttgcgccaag gatatcagga
    49741 ttgatttcat tccaattcaa taattcccct gcttcaataa gcagctctct tgaaactttt
    49801 gaaaaggtca aatccacatg tggctcacca aacagttttc cattaacata tggaaattca
    49861 agtagccact catccttgcc attgcgagct aactcattaa tatccaagat tgcaaagagt
    49921 tctttgatta cttcgttaaa attcgaacca tcttcagccg ttcttgtttt tagaacattt
    49981 gtaaaggaac ccttattcat aattcctgta tcttctgcaa acaacaagaa cagcactcga
    50041 atcaagaaca agttaaatgc atgctcgttg gcatcaggat tatctttagc caccacatca
    50101 tacaattttg caaaacgttc agcagctttt cggtcagctg gattttcagc ttgataatca
    50161 gccttctcaa tgccattcca cgctaagaaa aagtcagcat tagccggtag ctcttcaatt
    50221 ggaatattca aagtttcatg agttttcgta tcaattgcag caatatctaa ataatcgttt
    50281 accaagatat atcgtggttt agatttttgc tctttgattg attgagcaat cgcatcaatc
    50341 gcaatgacaa cgttgccttc aatttcttca taatgaagtt tgttcttaat cacaaatggt
    50401 tcaccattat cgaattttga ttttgcacgt gtaatcgacg ttttggggat atcgtacaag
    50461 gacaaaaatt gagtgatgaa atcatcgccc ctatttgaaa gtattttttt tacaccatct
    50521 tcaatttcag tgatgtttaa aactttattt tttgccattt atttaacatc ctccgcatct
    50581 ataacatctg aactaattat ttcactaaca tcaacattta aggcagcagc aattttcaat
    50641 aatgactcca ttgcaaccaa ctcattttta ccgagttttg ctattacatt gggactgacg
    50701 ccagatattt cacgaagttg agttttgttc atattcaaat caatcaataa cttccacaat
    50761 ttattataag aaattgacat actacacctc tattctactg gtatcttcaa ttatatcatc
    50821 tatgagctat cattattata cgaaacatga aatattttcg cgaacgcaaa ctatttttct
    50881 aaaataaaag ccttcagcat agatttacta aaggcgagat aacaaatatt aattccactt
    50941 tattttaacc ggaatcccca attgacgagc tagtttatca attttgattc tgacattctt
    51001 tttgttgaaa tttgcataaa cccatactcc atcagctacc ctggctgaat ttggtcttcc
    51061 gttatacatg gatggtttac gtcctaaaat tatagcgaac gacggcgtct catcgacaat
    51121 taattcttgc agttgattgt tgtagtttcc atccgcaaat agctgagaaa caaaatcaac
    51181 caataaacct ctagcagatt tcgaagatat ctttttccca tcatactcaa tttcatattg
    51241 tccagatgaa ctaaattcat aaatttgggt ttcactgtct tcggaagtac caaacaaatc
    51301 gatatttgca atttcatcta taatttcaaa cactgtgtca tatagctgat ccgattttgc
    51361 tttttgaagt ttatcaatat acgaagtatt accagcagtt gaattcatca tttcatagtc
    51421 agatttttct tggaaatctt ggataagtcg tttttcaaga taatctgtat ctgcttttga
    51481 cattttccca tctgctcttg taaagaataa aactgattca acccaagctt tatattcctt
    51541 gagaaagtgt tgagataaac gaagtgaaat tgattgccct gctgcctgac ccacgtaacg
    51601 cttattacca ccaatcaaaa cataaacagc tggataattt gaaaaatcct gttccttcaa
    51661 atacggcaaa tcactacgac tcattgaaac caagattgct ggctcgcgtt caaaataaga
    51721 aatattatct ttctgtgtat ctgaaaatat aaaaatttgc ttcataagtt tgtctccctt
    51781 gaaaactcga taatatatta tatcacgtaa ccgttatcaa aatcatctaa cctatcaagt
    51841 ctacggtgta actcctatca actcaaagct ccatagcatt tccatcaact ctacgctcac
    51901 ccaaaaatcg tcatttcaac acgaaaaaag agcaatccgg aaaccatccc gaatcgctct
    51961 aatccttgtc atatcaacgt tccacacgtg taagcagtcc tacacactcg atgtgatggg
    52021 tttgcgggaa aagatctaca ggctgaactt tcttcacggc ataacctgct tctgcaaata
    52081 acttagcatc tcttgcaaaa gttgccggat tacatgagat atagacgatt cgttctggtg
    52141 acatttcgat tgctgattca ataaaatgat gatctaatcc ttttcgtgga gggtcaacta
    52201 cgatcacaga tggtttgatc cctttttctt gccatttttt cataactttt tctgcttttc
    52261 cgacttcata atgaacattt tcgattttat tatttaaagc attgaactgt gcatcttcaa
    52321 ttgcttctgg aataacttcc atcccataca ccttatccac tctgtctgct aaagacaagc
    52381 caatcgtacc tattcctgaa taagcgtcga tcacaatgtc ttttttatct aactcggcaa
    52441 actcgatggc tgtttggtac agatactctg cttgttgagt atttacttga taaaatgact
    52501 ttgctgagat tcgataggtt tttcctaata gctgatcttc gatatagtct ctgccatata
    52561 aaactttttc tttgtctcct aaaatcacat ttgttttttc ttcattgata ttttggatga
    52621 ccgaaaccac ttctggcaat tcttcatgaa tagcttcagc aatttccttg gatttaaaga
    52681 atttttcttt acgagtaact aaaacgacca tcatttcatg ggaataatga ccacgacgga
    52741 taatgatgtg acgaagctgt ccttcatttt tttcttcatt atatcctcgt acttggaacc
    52801 gttgcaagat atctctgact actttgattg cttgatcgat gacaggatct tggatataaa
    52861 aatcttcgat agggactaga tcatggctat tctttttgta aaaaccggtg gctaactgtc
    52921 catcgatacg gcgaacaggg atctgtgctt tattacggta gccgaatggt tctgccattc
    52981 cgaccgttgg aagtacagga acttctggca tcttagcaat cttcgccatt acatgctcta
    53041 cttgtttttg tttgaagaca agctgttgtt catacttcaa atgggctaaa ggtgcgatac
    53101 ctatgcgaag taatgcattg ttttctacag gctgacgatc aggtgattgt gtaataaatg
    53161 tttcgacttt tccatatcca aacttattgc ctgcttttac tactagtatt tctacttctt
    53221 ctccagggag tgcattttcg ataaataatg gataaccatc gatttttgct acccccattc
    53281 cttcataact caagtcgacg atttcgactg tatatcgttc gtttttcttt actatttgct
    53341 gttctgtcat attttgctcc tttaagccta tttttcaatc ctactgaact ctactataaa
    53401 tcagctaatt acacaactaa aaccggcact gaccttacta cgttcagcac cggcttttga
    53461 ttaattcttt tgattaggtg tttcgtctgc tatttttccg tcaccgtcga tatcttcatc
    53521 cgttaaacgt tcgacttctt ttacgaattc tttgctgaca acctctaatt cttcttcatc
    53581 tgtacctgtg attgcttcat caggaatctg atcggtattg gcaaagaact caatgtgttg
    53641 atgaagattt tggaaatgca tcggtgcttc cccgccgtat tcaccatcta agttgatcat
    53701 tactggtcga ttgatatcat cttctgtttc aacgaacaaa ctgcttgttt tggtatagat
    53761 tattcttgga tcatcgacat gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat
    53821 ctcaaagata tttgctgtct tcacaatgat taaagaaaac ttcccatcat ccaatttggc
    53881 atctggcgcg atctgttcga atcctccaac agagttcgtc agccctagga aaaacatcga
    53941 agcatttccg atatattccc cttcatcata cttcaagcgc attttgattg gctttactcg
    54001 agggagcatc tctgctcctt tcgcaagata agcaagataa ccaaaaatac tctttagttc
    54061 tgatggaact tcataagtca attctgtcaa atagccacct gcagcgatat tgatgaagta
    54121 actgttatca gaaaggccga tgtccatttt tacggtttgg tgttttttta tcacttctgc
    54181 tgctgcttta atattatctc taggaatttt taatgctcga gcatagtcgt tcgttgtacc
    54241 agctgggatg atggccattt taggacggtg ttcaagcgga gcgattccat taaccacttc
    54301 gttgattgtg ccatcgccac cagctgccac tactagatca aaacctagtt ctgcgactct
    54361 acatgcttca ttcttggctg aatctggttc tggtgtagtt gcataggcgc tggcttcaaa
    54421 tcctgcttct tctaatgcct gtaggatatc cgccaaattt tttttcaata attcttttcc
    54481 cgaggtgggg ttataaatta cgcgtgcttt tttcattttc agcctctatc gtttcgctaa
    54541 ttcatcttta agtagcttat tcacgacacc aggatttgct tgtcctttag ttgctttcat
    54601 gatttgaccg actaggaagc caaccgcacg atcttttcca tttttgaaat catcgacaga
    54661 ttgttggttg ttatccaaaa tttcattaat gattggcagt agtttgttcg gatcagataa
    54721 ttgcacccaa ccattttttt caactacttc ttgggcttct ccgccatttg tgatcaattc
    54781 acggaagact tttttggcga tttttgagct gatcgttcca tcggcgatca aacgaatcat
    54841 tcctgctaag ttctcaggtg tcaattttgt ctcagctaat tccagtttct cactgttcaa
    54901 gtaagcagaa acttcaccca tcaaccagtt agatgcttgc ttggcatctg caccattttc
    54961 caaaacagct tcaaaaaaat cagacatttc ttttgtaagt gtcaatacca ttgcgtcata
    55021 ctctggaagt ccaaactcac ggatatagcg ttcgcgacga gaagctggca tctcaggcaa
    55081 actagctttt actttttgaa tccattcgtc atcgatcacg aatttcggga tatcaggctc
    55141 tgggaagtaa cgataatcac tagacccttc tttgacacgc atcaaaactg ttttattcgt
    55201 tgtttcgtca aaacggcgtg tttcttgttg gatttctcct cctgaaagaa gaacttttgc
    55261 ttgacgtttt tcttcaaaag ctaaaccttt tttcacgaaa gataaagagt tcaagttttt
    55321 caattctgct ttcgtaccga actcttcttg tccataaggg cgaagagaaa tattggcatc
    55381 acagcgcata gagccttctt ccattttcac atcgctgact tctgtaaatt ggataattga
    55441 tcgcaatgct tctagataag cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac
    55501 gatttcgatc aaaggcgtgc cttgtcggtt caaatcaacg taagaataac cgccaatacc
    55561 atgcatgttt tttccagcat cttcttctaa atggacacgt tcgatccgga tttttttctt
    55621 tttaccttct acttcgattt cgatccatcc gtcatgaccg attggttgat caaattggga
    55681 aatttgatat gcttttggat tatctgggta aaagtaattt ttccggtcaa aatgagtatc
    55741 tttagagatt tcacagttta atgccaaagc tgctttcatc ccgaattcca gcgcagcttt
    55801 attcattact ggcagcacgc ctggataacc ccagtcgatc acgtttgtat tgctgtttgg
    55861 ttctgcacca aaatgagctg gcgctggaga gaaaattttt gagttggttt ttaactcgac
    55921 gtggacttct agtccaatga ctgtttcaaa attcattcgt ctttccctcc taaaatagct
    55981 ggttgttgtt tatggaattc tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt
    56041 ttttcatcaa aataattacc gatgatctgc aatccaactg gtaatccttc tgataaacct
    56101 gccggtacag acatacctgg aagtccagcc aagttgacag ggatcgttaa gatgtcactc
    56161 atgtacattg tgattggatc gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa
    56221 gatggcccga tgatcaagtc ataatctgca aagacttttt caaaatctcg tttgatcaat
    56281 gtacggactt gtcccgcttt tttgaagtaa gcgtcataat aaccagcact taaggagaat
    56341 gtacctaaca tgatacggcg ttttacttct tcaccgaatc cttctgaacg tgagttgaca
    56401 tatacatcat ctaatgattt cacattttct gagcgataac cgtagcggat accatcgaat
    56461 cgttgcaagt tggaacttgc ttctgatgag gcaatgatat aataaacagc cacaccgtat
    56521 tttgaatgag gcaagctgac ttcttccaca acagcaccta atgaacggaa tgtttctgct
    56581 gctttcaata cagcttcttt tacgccaggg gccacacctt cgcctaagta ttctttaggt
    56641 aaagcaatct tcattccttt gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa
    56701 acgcctgaag aagtaccgtc tttttcgtca taaccgctga tagctgtcaa tgctaatgca
    56761 ttatctttca ccgtacgggt cattggtccg atttggtcaa gagatgaagc aaaggcaatc
    56821 aaaccaaaac gtgagacacg gccataagta ggtttcatcc ctacgatgcc gttaaaagct
    56881 gctggttggc ggatacttcc cccagtatca ctacctaaag aaactggaat ttgtccagct
    56941 gcaactgctg ctgcagaacc accagaagag ccaccaggta cttttgttgg gtcccaagca
    57001 ttttttgttt tcttgaaata agaagtttct gtacttccac ccatagcaaa ttcatccatg
    57061 tttagcttac caacagggat catatctgct tgatagattt tatccatgac cgttgcatca
    57121 taaattggtt caaaattgta aaggatctta gatgcagcgg ttgtcaagat atcttttgtt
    57181 acgatattat ccttgatacc aattggaata ccagcaagga cgttgctttc atcaatccct
    57241 ttttcatcta aagcttttgc ctgttccaat gctttttctt cgttcaatgt aatgaatgct
    57301 tcgacctcag gctctgtttc tttgattcgt tcaaatgttg cttttgtcaa atcttgtaca
    57361 gtgatttctt tcgatacaag catatgatgc agttcttcta gagaacgatt gtgtaattct
    57421 gtcattatgc gccagcctcc ccattatcga tgattgcggg tactttgata aagccgtctt
    57481 ctgtttccgg cacatttttc aataattcat cgcggttcca tccagcttgc gcccgatctt
    57541 gtcgcatcac gtttactgtt tcaaccacat tcgacgtaaa tggaacacct tctgtatcta
    57601 cttcttccag catttcaacc atatcgatga ttttacctaa ttggtcggta aaacttgcaa
    57661 gttcttcatc tgcgaacgca agttttgcta atttggcaac gtgtttcact tgttcttcac
    57721 taatcgccat actacccctt ctttctaaac gagttaaaaa ctttgctctc tattttaaca
    57781 caagagtgcc gtgtctgaaa ttgtttattg aaatttgtct gaaaaaactc atttttcatt
    57841 cttctactga gcgactttta gccaatgcat ctgccatttg atcttctgtc caaacttcga
    57901 ttcctaattc ttgtgctttg gtcagcttgc tgccagcatc ttctcctgct accacgatat
    57961 ccgttttttt cgatacgcta ccagtcactt tcccgccaag attttcgatc gtctcttttg
    58021 cttcttcacg agtaaatcgt gttagtttac cagtaaggac tactgttttt tctttaaaag
    58081 gagactcgac ttcttgcaat tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg
    58141 cttgttttaa ttcattcatc aattcatgga cttcttcgtt actgaaataa gtgactacac
    58201 tgtcggcaat gatatcaccg atcgtatcaa gcgcaataat cgactcatag tcacttttac
    58261 tcaatgtttc tagatcaccg aaatgttctg ctaaaatttt tgctgctttc gctccgacat
    58321 gtcggattcc taacccgaaa atcaaccgct cgaccgaatt atctttgctg ttgtcgattg
    58381 ctgtcaaaat attatttgct gatttttctt ttattttgtc caacgtcaat aactcttctt
    58441 ctgtcaattt gtaaagatca gcaacatctg ctactagttt tttatcatac atctgttcta
    58501 gtacgcgcgg ccccaaccca tcgatattca tcgcatttct tgaaacaaag tggttcaaac
    58561 cttctttcat ttgagcaggg catttaggat tgatgcaccg taaggctact tcttcatcca
    58621 aatggacaag ttcacttcca caaactggac aatgtgtagg caattggtat tcttcgctgt
    58681 ctttaggtcg tttatctaac acgacttgcg aaacttctgg gatgatgtct cccgctttat
    58741 aaatcagaac tgtatctttc aagcggatat ccttcaactt aatataatca ccgttatgca
    58801 ggctggcacg gcttactgtc gttcctgcta ctcggactgg tgtcatgata gctgttggcg
    58861 ttactacccc tgttcgaccg actgtccatt cgatgttttc gattagcgtt tcaacttctt
    58921 ctggcggaaa cttgtaagct gctgcccatc tcggtgcttt gacagtaaaa ccaagttgat
    58981 cttgtagcga gaattcattc actttgatga cgattccatc gatttcatag ggcaaatcca
    59041 cacgtttgtc atgatattct tcaatgtatg accacacttc atcaatcgaa tgacatagac
    59101 gtttttcccg attcgtgtgg aatccaattt tttctagctc ttctagcgca tcatactggg
    59161 tttttgcttg cataggaccg aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt
    59221 tcgctgtgat tttagaatct aactgacgca aagaaccagc tgcggcgttc cgaggattcg
    59281 caaagatatc ttttccctct gcttctctgt cttggttcaa ttggacaaat gaccgttttg
    59341 gcatgaaaca ttcgcctctt acttcgatgt tcattggttc ttttaatttg attgggaccg
    59401 accggacagt ttttaggttt tcagtgatgt tttctcctac agttccatcc ccgcgagtcg
    59461 cacctctcac gaaatttcca tcttcataac gcaaagaaac ggaaagtcca tctattttta
    59521 gttcacagca atagtcaact acacgtccca ctgctttttg tacacgttga tcaaaagcta
    59581 tcagctcttc tttgctgaaa acgtcgttca aactgtaaag cggaatatca tgagtgactt
    59641 tttcaaaacc ttctaatact tttccgccta ctcgttgagt gggagaatca gacgtgatca
    59701 aatcaggata ttgttcctca attgctacta attctgcgta ttctttatca taaacatagt
    59761 cttccactgt tggtttatct tctacgtagt attcgcgaga ccattggttc agtcttgtac
    59821 gaagttcttc tgctcgttta gcagcttcgt caatcgtcat ttcatgttcc atcatttacc
    59881 ctccactact aagctttttc aattggcgca aaggcagcca aaagacgttt tactccttga
    59941 cttgggaaag ccacatctag ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt
    60001 cctagacccc attttttgtg tttgacttta tctccaggtt tccagctgtt attttcccct
    60061 ccagtagcac ttttagaggg aacaaccgct gtctctggct gtttatagct atatgctgtt
    60121 tttttagcga atacaggtgt ttttttaggc gtaggacgca tgccttcgat ttccagtaac
    60181 tcctgatcga tttcttctac aaaacgagaa ggtcggttat attgcgtccg tccatataat
    60241 gttctggaga aagcatttgt taaatacaac gcttcttctg ctcgcgtgat ccccacatat
    60301 gcgagacggc gttcttcttc cagttcgctt tcttccatca atgctcgaga taaaggaaaa
    60361 accccctctt ctagaccaat caggaataca actgggaatt ctaatccttt ggctgcatgc
    60421 aaagtcatca atgtcacttg ggaagcatct tcttctaaat tgtcaatatc ggaaacaagt
    60481 gccagatcat ttaagaagac cgttagtttt tcttctggtg catcagcgtc ttcttcattt
    60541 tgctgttcga attgtttgtc gaattcttgt gtgaccgtta aaaattcctc caagttttcg
    60601 atgcgtgctt gtgcttctaa tgtattttgg atttttaagt cttctagata tccgctacga
    60661 tccaaaacct ctttcgtcaa ttctgtcact gtcaagtatg ggatcatctg agtgacttct
    60721 tggatcatct ctccaaatgc acctaattgc tgtcctgctt ttccaccgat atttgccaga
    60781 tccacatttt gcgctgcttc aagcagtggc cattcatgaa gagaagcaaa actgcgtagt
    60841 ttttcgatag atcctggtcc gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg
    60901 attgaatctt gaggatttgc cagcacattc aaatacgcta aaatatcttt gatttcttta
    60961 cgatcgtaga acttgtgccc tccaaccatt ttataaggaa tatttgcttt caacagagtt
    61021 tcttccatta cacgagattg tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc
    61081 cgatgattgc ttcgaatctc ttcttgcatc cgatctacga taaaacgggt ttcgtctcgt
    61141 tcattatctg cacggtagta agtgatcttg tttccttctt tgttttctgt ccacaagttt
    61201 tttggttttc gattgctatt gttttcaatg acttggtttg ctgcgctaag gatgtttttc
    61261 gtggagcgat agttttgttc tagcaaaatt actgcagcat ctggataatc tttttcaaaa
    61321 tccaagatgt tctgcatatc tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc
    61381 acgacgcaca ggttcctaaa tctacctgcc aataaattga ctagcgtata ctgtgcgtga
    61441 tttgtatctt ggtactcatc tacatgaata taatggaatt tgttttgata atatgtgagt
    61501 gaatcaggat gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc aaagtccata
    61561 cattgattat attgaagctc tttttggtaa gcagcataac attttgccgc aatctcttcg
    61621 taataactgc cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga
    61681 gagattgttc ctaatatact tcgaggatca tattttttcg gatcaatgtt cagttctttt
    61741 agtatccgtt tcatcaaggt cagctgttca gaagaatcga tgattgtaaa gttccggtta
    61801 tacccgataa aatcaacatc tcttcttaaa atacgcacac acatcgagtg aaaagtcgat
    61861 acccagacat cttcgcctcc agatgcgaga atcgcattga cacgttcttt catttctttt
    61921 gcagccttat tggtaaaagt aatcgctagg atgttccaag gattgacttc tttttcctca
    61981 atcaaatagg caatgcggtg tgtcagcact ctcgtctttc cacttcctgc acctgccatt
    62041 agcagtaatg gtccgtcagt gtgaagaaca gcttctttct gacgggggtt catcccgttt
    62101 aataattcag ctttggatga cattaacaac atcctttctt ttatcaatct tgttaattat
    62161 agcattttct tggctttcat taaactagct agttcaaaat catttgtttc tttttatgga
    62221 tttttccttg tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg
    62281 gaaaaaaaag gaaaaatcaa gaaattagat aagactcaga tatattttat tctaaaagga
    62341 atatttgttg gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag
    62401 ctaagcgatt tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt
    62461 ggcctagcta tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa
    62521 ccggatatca agggaagcgg gattccacaa gtagagggac agcttcgcgg ggaattatca
    62581 atgaactggt tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg
    62641 ggcctttttc taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga
    62701 gctggacaat tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca
    62761 agtagcggac ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa
    62821 gaaatccatc actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact
    62881 gctaacttcg tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt
    62941 ccttctttgc cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc
    63001 ttaggtttta tttatcagaa ggtcttgctg gctttgccaa aattttacaa ccgattgcct
    63061 ttaccagctt attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta
    63121 tggcctagta ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg
    63181 ccaatcatta tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac
    63241 ggtggtggtc ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt
    63301 agcttttatg gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt
    63361 gtcgttattg ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt
    63421 attttagtga cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct
    63481 cttgttgctt atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg
    63541 gaacgtctac ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat
    63601 tttccagtaa cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct
    63661 aaaaatatgc tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat
    63721 acgatcatga atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga
    63781 atcaagcagg aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga
    63841 acatggaaaa actattttac aacaactatt catctacact acttgattct atatgattct
    63901 ataaaaaaag aagagactgg gaaaaaccag cctcttcttt tttatttcta tgcttctgct
    63961 gctgcaggag cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca
    64021 ataaacacca aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccatga
    64081 ggagccagta atttgatccc gaaagctccg actaatccgc cagttaaagc agaaccaata
    64141 ataaaacttg gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg
    64201 acaaacgata gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat
    64261 ttattcttaa acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc
    64321 ataacagaag ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta
    64381 ccaaacacat aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct
    64441 aataatgcac caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat
    64501 gcatcattga ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt
    64561 aagccaaaca ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc
    64621 ttgaataatt tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca
    64681 cctaagaatc ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa
    64741 ccagggcggt caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca
    64801 aatgcagctt gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt
    64861 gataattgat catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg
    64921 attcctccac caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa
    64981 attctttgtc cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca
    65041 ccatggaata ctggtgcagt accactaagg gctagattga tcagctcttc tggtttacgg
    65101 ataccatcac tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact
    65161 tttttgtctg ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg
    65221 tttttgattc cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct
    65281 tttttcttca gagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta
    65341 acggcaacaa cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt
    65401 tctgcttttt cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct
    65461 tctggtgtat ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat
    65521 agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa
    65581 aacagataag ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg
    65641 aataaaactg ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca
    65701 ccaagacctg tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata
    65761 tcggaaatcc ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta
    65821 tcagtcgctt gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg
    65881 attatcctcc ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa
    65941 tcaattcgcg agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc
    66001 atttaaaggc ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccggcaatca
    66061 ttgaatcccc tgcacctact gaatttttca atgggcgaac taatacattc gttttataag
    66121 ttccttgttc cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg
    66181 ccccttcagc tagcaatttt ttaccatacg gaatgatttc ttctacagat tggaaggttg
    66241 tattgtaaag ctcagctaat tcgtgattgt ttggtttgat caacaatggt ttcttaacaa
    66301 gtgcattttt taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa
    66361 tgatatcaat cagttcttga taaaatcctt ttcgcaggct agccggcgcg cttcctgaca
    66421 atacgacgat atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt
    66481 catctgaaat agatgggccc aaaccgttaa tttcagtttc agtatctgat tttagtttga
    66541 tattgattct tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa
    66601 gccacttcga aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag
    66661 aatcgatccg tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt
    66721 cttccttcat tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa
    66781 ttgatggatt aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc
    66841 acggaattca ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa
    66901 aggtgcaaca gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt
    66961 ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc tcttctgggt ccggagtcgt
    67021 gaatccttgt tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt
    67081 ttttagttgt tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc
    67141 acctaaaata atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt
    67201 tgtcactact gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc
    67261 agcgtctaaa taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg
    67321 ctgtttttcg tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa
    67381 ttgctctttc ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg
    67441 taaatcacgt cttatcgttg attcagaagc attaaaaagg tttacaagat cttgtgattt
    67501 cacgattttc tgctgttcaa gcaagtctag gattttttgt ctacgttcct ctgtaagcat
    67561 tttcacgatc acctcaatac aaataatagc atattttttt tctactcgca atataaatca
    67621 gtcaaaaacg ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcaaaaa
    67681 gactggtttt ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt
    67741 cttgtttttt tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa
    67801 agtcccatcc cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga
    67861 acaaaaaaga gacagacgga taataatgat gccggaacca ttattcgata atagagttgt
    67921 ttatccattt tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta
    67981 atctaaaaat tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg
    68041 ccgctttttc tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa
    68101 cggtaattct gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg
    68161 atatttttca tctgttacct ctacttctag atattcccta atataataac aataagcttc
    68221 gattccatta tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgcgagcg
    68281 attctcgtca acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc
    68341 tttataatct ccttgttgaa tatcataact ttttgttaca aaaaatgaag cattcttttt
    68401 aggaacaggt tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta
    68461 aatgtattca acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc
    68521 aaacccaagc tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt
    68581 aaaggggtct cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc
    68641 tacttgttgg atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata
    68701 atacatttcc caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa
    68761 ttggttgcaa tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat
    68821 ttcatccttt ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa
    68881 ctcgctataa gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa
    68941 gtctcgaatc aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa
    69001 taattgatta tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca
    69061 actttcgtct cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg
    69121 atccgcttgt tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat
    69181 agcccaatcg cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt
    69241 catcaataat ttatcttcgc caatcatttt ctcacctctt taaaatcttt ttataattta
    69301 attataacca acgataaaga aaaactaaaa ctttatgtct tagaaaccaa tttggacgat
    69361 tacatgatag actaagtaaa aagaaacaaa gaggtgttta caatggggtt gaaatttgaa
    69421 cgtacagata atttagataa acttttcgaa tcatttgctg tcgatccgga aaaaacagtt
    69481 gcaccaaaag aagagatcga tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa
    69541 gacaagaaac aaaagtaagt taagctcaaa aaaagactta ccagccaaac ttttatgaaa
    69601 gagtttgact ggtaagtctt ttttattctt tttatgattc aattgcttgt tcgattgctg
    69661 cccacgcttc ttcttttccc tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc
    69721 gatcaaaatc caacgctttt ttgatttcgc tctcatgctt gttccattta ccacgaggga
    69781 tcttatctgc cttagtggca acaacaatta cagggatatc ataatacttc aaaaattgat
    69841 acatctggat atcttcttga gaaggcttat gacgcaaatc tactaaagaa acgaccgcac
    69901 gtagctggtc tctagtcgtt aaatatgtct cgatcatctt tccccatttt gctcgctcag
    69961 ttttggatac ttttgcatac ccatatcccg gaacatcgac aaaatgtaat gcgttctcga
    70021 tcaaataaaa attcaatgtc tgggtctttc ctggcttacc ggaagtacgg gctaaattct
    70081 ttcgatcaat caatgtgttg ataaaagaag atttccctac atttgatcgt cctgccagag
    70141 ctatttctgg caattgtgta tctggatatt gttttggtgc cactgcactt atgataattt
    70201 cagcttgatg gactttcatg gtctctctcc ttaacaaggt gatgaataaa gcaagcggct
    70261 gtcgcaaaag ccaaaatgac ttttacggca gcctcttttc atcaacccgc ttttttctct
    70321 gaatctttat agattactgt tggctcacca gacaattcag ctgcttcttt tgtgataatc
    70381 actttttcga tgttttcatt tgaagggata tcaaacatca catccatcat gatttcttca
    70441 atgatggaac gaagtcctcg agcaccggta ttgcgttcga ttgctttttt cgcaatggct
    70501 ctcaacgctt caggttcgaa ttcaagttcg gtattatcta gagaaagaag tttttgatat
    70561 tgtttgacta gcgcattttt cggctcagtc aagatccgta ccagatcatc tgttgtcaat
    70621 ttctctaaag ctgccataac aggtaatcgt ccgataaact cagggatcaa tccgaatttt
    70681 agtaaatctt caggaatgat atgctgcatg acactttcgt cttcagaaag tttttggttt
    70741 tgtgtaccaa atccaatcgt cttttcgccc atacgattct tcacaatcgt ttcgatgcca
    70801 tcaaatgcac cacctacgat gaacaatatg ttcgtcgtat caatctgaat aaattcttga
    70861 tgtggatgtt tacgtcctcc ctgaggtggc acgcttgcaa cagttccttc tagaattttc
    70921 agcaaggctt gttggacacc ttcgccggaa acgtcacgag taatagatac attttcgctt
    70981 ttacgcgcaa ttttatcgat ctcatcaata tagatgatcc ctttttctgc acgttccaca
    71041 ttgtaatcag cagattgaag caatttaagc aaaatgtttt ctacatcttc ccccacataa
    71101 ccagcttctg tcaaacttgt cgcatctgcg attgcaaaag gtacattcaa tgtttttgct
    71161 aatgtttgtg ctaaaaatgt tttaccagac cctgtaggtc cgatcaagca gatattgctt
    71221 ttttgcagtt caacatcatc tgtgttttct tgtgcttgct gattgacacg tttatagtga
    71281 ttgtatacgg caacagctaa cgagcgtttt gcgcgatctt gtccaatcac gtagttattc
    71341 aatacttcaa gaatttctag tggtttaggg acttcagtga attcgcgtac agcttcttca
    71401 taaaattctt catcaatgat ttctttacat aggtcgatac attcattaca gatgtagacc
    71461 cctggaccag ctacgatctt tttcacttct tcttgggttt tcccgcaaaa agaacagcgt
    71521 acggtctcat tgctacttgg attgtcatac atggtcttca ccccttaact tgcaaaaagt
    71581 atgcaagaaa ctatcaaaga ctttacagtc ttttactatc ataacatagt ttagtaaaaa
    71641 gaaaaagtgt ttaacaaagg atcaactgac ctataccttt gcatttttta ctcttaacaa
    71701 aaaataagga actgtgacaa aatcgtgtca cagtccctta tttcgaataa acaattttct
    71761 agaagtagct tcttcagaaa tgatttatta cttgatttct attctttcgc ttctgtcgct
    71821 gtacctgtga tcaattcgat tgcttttttc attgtcacat catgttccag catatcttct
    71881 gtcaacacgc gtttgatttg ttcgattggc atgttgtatg tttctgataa ttcttggatt
    71941 tctgcattga tttcttcttc tgttgcttga agattttcag cagctgcaat tgcttcgatc
    72001 acaagatttg ttcttgtgcg catttctgct tcaccttcga attgtttatg caagtcttct
    72061 tctgtagaac cagttaattg atagtacatt tcaggagaga taccttgacg ttgcatgttg
    72121 ttcaagaatt cgtccattga acgatgtact tcatcatgaa ccatcacgtg tggcaattca
    72181 acgatttctg cgttttcaac agccatacgg atcgctgctt catcttttgc atcgtccgct
    72241 gctttttctt ttgtttctac taattctttg cgatattttt ctttcaattc gtctaatgtt
    72301 gatacttcgt catcaacatc ttttgcgaat tcgtcatcta attcaggcac ttcttttgct
    72361 tttacttcat ggattgtcac tttgaagact gcttcttttc cagcaagatc ttctgcttga
    72421 taatcttctg ggaaagttac atttacatct agactttcac ctgatttatg accaactaat
    72481 tgatcttcaa aacctggaat gaatgagcct gaacctaatt caagtgagta attttcgcct
    72541 tttccgccat caaacgcttc gccatctaca aagccttcga agtcgattac tactgtatcg
    72601 ccattttcag ctgcagcatc ttctttgata actaattcag cttgtgcttc ttgttcacgt
    72661 ttgatacgtg catccacgtc agcatctgtt acttcacggt cttgtttttc aacaactaga
    72721 tctttatatt gtcctaattt tacttctggt ttcactgtta cttcagcagt gattacccag
    72781 tcttgacctt tttccatgct ttcaacatcg atttttggtt gagaaacagg atcgatgcca
    72841 gcttctttga cagcagcttc ataagcttct ggtaatacag cattcaatgc atcttcatat
    72901 aaagcttctt caccgtacat acggttgaat acttgacgag aaactttccc tttacggaat
    72961 ccaggtacgt tcaagctttt tttcacttta ttaaacgcag ttgttaatcc tttttggatt
    73021 tcgtcttgcg caatcgtaaa ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt
    73081 gcagtcattt aattccctcc gagtttagaa attcatttta tacatcaaaa acgtttcatg
    73141 cataccttta aatagtacac taaccctttc aaattgtaaa catattgaat ggatagaatc
    73201 cttgtttttc ataacatttt tatctttttc actgataaaa tctaatggaa gaagatcatt
    73261 tgctgcaaaa ttttttctct tttttttaaa aaatcttgtt tttcatcttc tgtaccttca
    73321 tacatcgttt ttcctgtaca cgcctctaca gtcagttgaa accagcgctt cgggtcttcg
    73381 atgtatgtat catagacagg ataaacaatt gcagattgca acgtgaattc ttcatttaac
    73441 acggaactaa gcagcgcatc ttcattaccg taacgatctg aaaatacaca taattgctgg
    73501 taacttgcct gctgctgcgg ctttggcaga tcttttggta tgactttgac taacttttct
    73561 tcaatcgtca aatagaaaac aggatggcat tcgttcaatt gcctaagtgt ctccagtaat
    73621 tttgcccttg ctaaaatatg gacctctggc gatacaataa gtttactcgc tgtttggata
    73681 aactcatgat aaggaagctg ttcgatctca tcaatagacg caagctgacg tgcaggttcc
    73741 aacgaaggaa gttcctccga tttattgtgg agtgccttga tcttctgcct ctccatttgt
    73801 ccggataatt cttctgcaaa ttggattttt tcaatgaccc gctgctcgaa agcttcataa
    73861 aaatctgtac ttgctatcaa ttttctagca tataaaaaat cttgtgtact gattaacaag
    73921 tcaaaataaa attcagcaaa ctcctcattt tgaagatact gtttttcatg caagagtgct
    73981 tgcttcaatg cttctttttt ctcgtccaac tcgtaaagac aaaaaacaag caggctatta
    74041 gcctcaaagc tttctttcaa ctcgtaagct cgttgaaata attcttttgc ctgctgatat
    74101 tgctggtttg ctagtgcttg ttgtgctact cgcattaaat tctcatattt ttctggaaaa
    74161 tcaatcggct tcacgcagtg tcctcctctt gctcatgttc agctcatgtt caatagtagc
    74221 aaaacaaaaa aagcatgaca attaaaaata actgaagcac cctctaaaag ctagatttta
    74281 gatctgactt ttagaaggtg ctttcaactg ttactgcttt ttattgctca tcagctgttg
    74341 acttcctggt tgaatgcttt gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa
    74401 tcgcctagat gttgcttttt ctgattaaag attgctggaa gataccctaa catcagcaaa
    74461 ggaaatttta gaatgaaccg gcaagcacct tctctaacta aaacggttga ccagctaagt
    74521 tcttcttcat caaagctgat gactcgaata ccaaagatca ttttcccaat cgtttgacca
    74581 tgattgaatt tcgtcaataa aataaaatac gctagataaa taagtaaggc tgtgaatccg
    74641 tagatactca gataagaatc tgaaaaatta agccaaccta gatttttggc aataccaagt
    74701 gttaaacgcg taatagctcc gatacacaaa agatcgacca agaaagcaaa cattcggata
    74761 aaaaaacctg catagaaata cttaggaaaa tcatttgctt ttggcggttc atttcttgtg
    74821 tccgtatact ttttccattt acgttgtttc tttttgattt cttcgggcgt caaaggctct
    74881 ctttcaaaag agctcagtac cttttgggaa aagttggagt caggtcctac agcaggctgc
    74941 tgttgcttat ctttttcttg ttccttattt tcatttgaaa gctctgacat gcttactcac
    75001 ctccgtaata atacattgct tttggtgatt ccgctgtccc tagattctca agaacggata
    75061 agatctgact agtctcagaa ggcttcaatc cctggaattc tgctagttta cttcctaacc
    75121 atgtactagc aaacccagat ggcgtggaag aatattctac tagttccgca tcttcaagct
    75181 gttgttcctt tcgcatagct tttaaagcat cttcaggaaa tcccaattca tcgactaaac
    75241 ctacttcttt tgcttggaca ccatcataaa tccttccatc agcaattttt ttgacttctt
    75301 cttcagattt attccgtccc tcgctgacga ttgctacaaa acgactatat gcgctgtcga
    75361 tatatgcttg aagcaccgct tcttcttctt ttgtctctgg tcgcgttcct gaacccatat
    75421 ctttcaacgc tccgctttta actgttgtat cttccacacc cagtttttct agaagtcctg
    75481 aataattcaa tcctgacatg atcacaccaa ttgagccggt cactgtttct tctgttgcaa
    75541 agattttatc tgcttgagca gaaatatagt agccaccgct agctgccatg tttttcatgc
    75601 tgacatacat tgggatatca tgttctttac gaatcgtatc caatttctta gcgatttccg
    75661 cactttcgta gacaccgcca ccaggagaat ttacttctaa aaagatacct ttgactgttg
    75721 gatcttcttc aatcgccttc agttgttcca taaaattttt atgattgtaa ccttcagaag
    75781 caaataaacc tgaagaccct cccgcagcaa tcgttccatc tactgataat tttacgattt
    75841 tttgcgtact gtctccctct tccaacacat ttgaagctag ttcttcatcc ccatataacc
    75901 aactgttcaa tccctcaatt tgatcgtttt cctgaggctt agtcaaacta gctgaaacta
    75961 acgaaaccac aaataatcct gcagcaatca gaactgctgc ccaacgtctc ttattcattc
    76021 tttctccttt ctaatacaag ttttttatga cgatcacagt ttctgactgc tcagaagtgt
    76081 cgaaccgctc tgctttttct tcccgcgcat acccggaaaa cgactctttt gtcaattcat
    76141 cttctaccgt aaactcacga gcttcagcaa cttccaaggc atcaggtgcg atttcttgga
    76201 tgctatccca gccagtatct aaaaatattt tcgtatcatc tgatacacca gctgatgctt
    76261 cgaatttgga caattgtttt tttaacattc ccagtgtcat gatctcaatt tttgaatcca
    76321 tttttactcc tcctctatcc gattactttc tattgtaccg aactcctgaa aaagatgata
    76381 gataaaaacc cattattttt tgacataaaa agagcatttg acaaagcatt tgatatatac
    76441 caatttattt tttattcttt ttttgttgac aagaacattc gcaaaaggtt atgattttaa
    76501 atgtttccag ataaaaaaca aaagaggagg atagttatac cttttaggat agcgccaggt
    76561 ctgtgtaaac agatgacgag gagagggttt atcgaaagat tcggcggatg gccctaggga
    76621 ctgcactcta cagaaaatcc acaaaaaata tctgcaaaga taaaacaaaa ggattttcct
    76681 gcagctggaa gcattcatac gaattcatta tagaaagtag gatgtttttt atgtgcggaa
    76741 ttgttggaat gattggatta aaaaatgtaa caccaggatt gattgacggt ctagaaaaat
    76801 tagaataccg tggttatgat tcagctggta tctttgtctc agatggcaca actgattatc
    76861 tggtcaaagc acaaggcaga attcaaaatt tgaaaaacaa aataaatatt gatactacag
    76921 gaaatatagg tattggtcat acacgctggg caactcacgg gcaaccttct gaagaaaacg
    76981 cccatccaca tacttcccaa agtggacgct tcgtgctcgt tcacaatggc gtaatcgaaa
    77041 actttgaaga acttaaaatt gcttatttag caaatgacca ctttattggt gagaccgata
    77101 ctgagattat cgctcatcta attgaaactt tcgcgaagga tactacaacg caagaggcgt
    77161 ttttaaaagc tttgcgggtc atcaaaggtt cttacgcttt cgctttgatt gatcggacag
    77221 cacctgatgt tatttacgtc gctaaaaata aaagtccact attaatcgga ctcggcgatg
    77281 gattcaatgt gattgccagc gatgcaatgg ctatgcttgc tcatacgaag gaattcgttg
    77341 agattgaaga tgaagagatg gttaccgtca catcagaaaa agttattatt caaaattttg
    77401 caggtgacat cgtagaacgt tcttcattcg aagcacaagt agattcttca gatatcgaga
    77461 aaggtaccta tcctttttat atgctgaaag aaatcgacga acagcctatt atcatgcgtc
    77521 gtatcgctca aaaatacgta acagaagaca atcgtgtctc tcttgatcaa aagctggtcg
    77581 atacattgtc tgatagcgat cgaatctata tcgttgcttg cggaactagt taccatgcgg
    77641 ggttagcagg taaacagact ctggaaaaat tgacacagat ccctgttgag gttcatttag
    77701 ccagtgaatt tggctataac acacctcttc tttcaaaaaa accattcttt attttcttga
    77761 gtcaaagtgg cgaaacagca gatagccgcc aagttctggt aaaaatcaac cgtctcggct
    77821 acccttcact tacgattaca aatgttgctg gttctactct ttcgcgggaa gcatccttta
    77881 cactgctatt gcatgctgga cccgaaatcg cagtagcatc aacgaaagct tatactgcac
    77941 aaatagctgt actaacttta ttagccaaag ctatcggtga taaaaaagaa tttgaaactt
    78001 ctcttgtatt cgatatcact catgaattga gtttagttgc taatgcgatg gaatcggtca
    78061 tcgcccaaaa agattattta gaagagttgg cagaagtata tctcagcgat acaagaaatg
    78121 ccttttacat tggacgtggc gctgactaca acgtttctct tgaagctgct ttgaaattga
    78181 aagaaatcag ttatatccag gcagaaggtt ttgccgctgg cgaattgaaa catggaacga
    78241 ttgctttgat cgaagaagga acaccagtga ttggaatcat cagtgaagaa gtgactggtg
    78301 cgcatactcg aggaaacctg aaagaagtcg aaagccgtgg cgctaaaaca ctagttattg
    78361 tctcagaggg attagaaaag gaaagtgacc agctggtgct accaactgtc cacccttatc
    78421 taactactct aaccacagtc gtaccgactc aattacttgc atattacgca acgctattac
    78481 ggggattcga cgttgacaaa ccacgtaatt tagcgaaatc agtgacagta gaataataaa
    78541 agaacaagga ccagtgtcag acatctgaca ctggtccttg ttcttttatt ttttatgaaa
    78601 atatctctta atccagtccg atttcttctt tgactactgc cgcgatttta tcgacatagt
    78661 aattaacttt ctcatcagta ggcgcttctg ccatcactcg aagtaatggt tctgtaccgg
    78721 aaggacgaac taggattctt ccttctgatc ccatttcagc ttctgcctct tcaatcactt
    78781 ttttgattgc cggaacatcc atcgcaccgt ttttatcact tactcggata ttcactaatt
    78841 tttgcggata gattgttacc tctgctgcaa gctctgataa tttcttacct gtctgtttca
    78901 tgatattcag taactgaatt cctgaaagca tcccatctcc agttgtgtta aaatctaaga
    78961 aaatcatgtg cccagactgc tcgccgccaa agttataatc gtttttgcgc atttcttcta
    79021 caacgtaacg atcaccaact tgtgtaacga catctttcaa accgattcct tccaccgctt
    79081 tgtggaatcc taagttactc atgacagtcg taacgatcgt atcttttttc aatcgatttt
    79141 tttctgccaa gtatctagca cagataaaca tgattttatc accgtcgaca atgtttccaa
    79201 gttcatccac cgcaataata cgatcgccat cgccatcgaa agctagtcca gcatctgctc
    79261 ctttttcaac gaccatctca gctaaacgtt ctggatgggt agagcctacg ccatcattga
    79321 tatttaaccc gtctggattt gtgcccatgg tataaaaatc tgtttcaaga tcggcaaaca
    79381 aacgattaac acttgttgaa gttgctccgt ttgctgcatc gacacaaatg gtcaaaccgg
    79441 aaaggtcacc gttgatcgtt tgtacaagga attgagaata tttcaataag ccttctggaa
    79501 attcttctac agttcccaat ccttcagctg atggacgagg caattcatcc acttcagcat
    79561 ctaataatgc ttcaatttct agctcttggt catctactag tttaaaacca tctgcaccga
    79621 aaaatttgat tccattatct tgtgctggat tatgagaagc agaaatcatg acccctgcgc
    79681 ttgctttttg caaacgtgtc agataagcaa cacctggtgt agagatcaca cctagctgga
    79741 acacttcgat acccaccgat aaaagtcctg aaatcagcgc ttcttctaac atttgacctg
    79801 agattcttgt atcccgccct actaacacgc gtgggcgttt tccttcttct tcatgctggc
    79861 ttaggacata acctccgcac cgtcccagct taaatgcaag ttctggcgta agttccttgt
    79921 tggcttcacc gcgtacgccg tctgttccaa aatatttacc catagttgta aaattactcc
    79981 tttaacttaa ttttctgagg aatttgttct gctcatttgt gttgattcgc ttgtttctga
    80041 tgtttcttct gtacttgtgg tcgagctgct tgtcccagat tccgaactgg aagaaatggt
    80101 tgttgtcgat ggttccgttc ctgaatcagc agatgaaata cttctactcg tacttgtatt
    80161 ttcttgtgtc cgactgctcg tcgaagcaga gctactgctt gacgtttgca acacgggtgt
    80221 aatctccaca ctaactgatt cgggcttgat acttaacccc tcttctactg gtatttcaat
    80281 tttacgagtt gttcgatgat tgatacctga gacattgatc ggtacagcaa tactgtcacc
    80341 gatcgatgca agcaaatcgc tagagccgct tacttctgct tccagttttg aaaaactgaa
    80401 atcatagctt tctaccccgg cagctggtgt tccttgctgt atcccataaa gtcgtaccga
    80461 cttgcttggc ttgatcgaat cagctctcac tgttacttgc actggatcag agacgatact
    80521 taatacttcc cctgctgcat tcagcgcctg tattgggacc tcactattaa tcccatcatc
    80581 tgtgaccatc gaagcgtcca catcagctac tactcgtttg atttctgcca atgttttgtc
    80641 acctgtagtg atagagactt ttttaggact aacggaagta tcatcaagtt catatccaga
    80701 tggtgtgaac gttgaaggaa tcaacgtttc cacatcgaaa tcttttatta cctttttctc
    80761 aaccgttact gtaatagtcg atggttcgat ctttgctgtg acagcactgc tcaaattttg
    80821 aatttttagc ggaacctcat gtgttccttc gcccagctta gtgatatctg ctgtgacgcg
    80881 gaacatccgt gtatcttcgt ttgcctctgc attcaactga actcggtttg cactggaaag
    80941 tttgacagtc actgcatttt catatccatg gatatagtag cgctttgaat cataagaaat
    81001 cttgattggc accccagtaa ctgtttgatt atacgcttca tttccagaaa gcgtatttgg
    81061 aacggactgg ccatttgcat tgaagtacag cgcaatactg aacagcaatg caataaaact
    81121 gtaaagaatg cttgtccgtt tctcttttga gatcatttgc gatcccctcc cttagtcata
    81181 ccgtctagga aagattgaat cacgtttttc ttgttttttg ttttctcttc agcaatcagt
    81241 tctctacgca aaattgccaa atattcttgc tgggataagc cagccatcag ttcattatct
    81301 aatgtaatgc tgacatcccc agtttcttca gagacaacga tggtaatcgc gtcgctgact
    81361 tcgcttatac cgacagctgc ccggtgtcga gtcccgaatt cttttggaat caacatactt
    81421 tctgatagag gaagataggc gcttgctact gcgatctttc cttcttttac aataactgca
    81481 ccgtcatgca gcggtgtatt aggaataaag atattgatca gcagctctcc agtgatatct
    81541 gcatctaatg caattcctgt ctcaatatac tcatctaatc cagtatgccg ttcgatagta
    81601 attagcgcac cgatttttcg cttggacata tattggatgg ctttgtcaaa agacaagatc
    81661 atcttctcat cttcctgctg ttcgcttttt gattgtttga aaagcgaact gcgtccaaga
    81721 tgttccagtc ctctccggat ttccggctga aaaatcacga tagcagctat caccccgtac
    81781 gtaatgactt ggttcatcag ccaagacaag gtgtgcagac caatgatttc cgctacgatt
    81841 cggataacaa taaaaatagc tacacctttc agaagctgaa tcgcttttgt gcctcgcagc
    81901 agttggatca gtttgtatac aagataccag acaaccagaa tatcaagtat gttcaccaaa
    81961 aaattcagag agaacaagtc accggaaaac atttgctgcc aatagtccaa attaaaaagt
    82021 tccccgattt gaaatgacat aaaaagcctc tcttctcttt tgagatttcc tctctagtat
    82081 atcatatgtt ttaattttag aggctgatct gctcgtattt ttcttgagaa aaacgcataa
    82141 ttttcaggaa aattgcaagt atgcgtttta tttttctctg caataaatat ttttaaactt
    82201 cagagaatta ttcattatat tttttctgtt atttttcctt tttttctttt gtagcttgtt
    82261 ccattttttt acgtcgttct gctctggcag ctcttctttc ttctgcttca aggtccgtcg
    82321 ttttttcttt tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct
    82381 tgattttttc taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtctt
    82441 ggatagtctt ttcaaataaa gcttttgcaa gatttggatt tgcgttctct agcgaggaaa
    82501 agcgaacttg tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca
    82561 aagtcattgg gttcttgttt ttttcgacca ttgcagggtt atatcgatat aatgaccaat
    82621 agccagaaga aacagcttct tttgcttctt taagcgattc tttcattcca ccaagcaaac
    82681 catgtgtgat acaaggcgta taggcaatga ttaaagaagg tccaggatac ttttctgctt
    82741 cttcaaaagc ctttatcgtc tgcatttgat tggcacctga agcaatttgc gccacataaa
    82801 catttccata tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag
    82861 ccgaaaattt agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg
    82921 aatagacttc gttatccaaa acaagaatat tcacgtctgc tccgctggca agtacatgat
    82981 cgatcccgcc aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt
    83041 tttttacgaa tagatccttt ctcttgtata tatcctccag ttcagggaca attgatcgtt
    83101 cgctttcgat cgcagcttgc agttttgctg ctctttgctg cgttccttcg ctctcaaaca
    83161 tgtgtaccaa ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt
    83221 ctttgattga acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac
    83281 caaattcagc attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat
    83341 tggtcgtata aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat
    83401 tcgcgatcat cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc
    83461 cacagcctga gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga
    83521 cagtattctt tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat
    83581 tcacagcttg ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg
    83641 gacaagcctc tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt
    83701 attttacgcc gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca
    83761 tttcctcttc atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt
    83821 cattacacat cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct
    83881 tttccataga agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg
    83941 taccaacaga gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat
    84001 aaccagtgtt ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga
    84061 tcgcgacttg atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga
    84121 tagccattga tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct
    84181 catcaaaagg aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc
    84241 ggcgtcccaa accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt
    84301 tattttcagc caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc
    84361 tccaaagagt attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc
    84421 tatgcaggta agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg
    84481 aacgtatcgg cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg
    84541 aatcataata aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag
    84601 ctgatttatt tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg
    84661 tttgcggttc tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat
    84721 ccacgatccc tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa
    84781 aaactgctga gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa
    84841 taacagccgg gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt
    84901 ctccacctgc ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag
    84961 gcaacttttg cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac
    85021 ctgttttccg tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg
    85081 atcccataga aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat
    85141 aagaagttcc tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt
    85201 tatcataata ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg
    85261 taccactaac agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct
    85321 cctgattgat cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt
    85381 gactcgtacg gaatccatca aagaaattca aaaaaggtac ttctccttca atagaagcaa
    85441 gatgggcaac aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa
    85501 atcctgtttg tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg
    85561 tcgtcactgc ccgactagca acatggaaaa cacttggcag tagttcccca gcaatcttgt
    85621 acatattcgg tatcatcaaa agcagccctt gagaagcggt atatgtagtt gtcagtgcac
    85681 cagttttcaa tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta
    85741 cttttactgg ttgaccgaat acattttctt ttcctgctgc tgaccattga tctactaatt
    85801 cagccatggt cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag
    85861 aaatatatgc ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc
    85921 cttgttcata ttctctctta ttatctgttc cattataacg taaaacattc cattttttct
    85981 tttatttata aaaatatttc acatatgtct atttgaattg gtcatctaat ttttagacaa
    86041 acagtctaaa tcgttgcctt tttttgtcag acatgtataa actataagcg aaagaggtgt
    86101 tttaacatgg atatatcagt gattgatgca acgaaaacaa atacacaaaa aggaatcctt
    86161 tatggttcat cgaatgctcc taaaaaaatg gtcgaattca tcaacctatc ttgtccgtat
    86221 tgccgacaat ggtttgaaga atcttatgag ctattagaag aagcagtcca aagcgggcaa
    86281 ttgcagcgag tgatcaaatt gttcgataaa gaaaaagaaa gcttgcagcg aggaaacgtg
    86341 atgcaccggt atctcacaat cagcgatggt caaaaagcga tcaaagagat caaacaaata
    86401 ttcgatactc aagacgagtg gaaacatctt tctctccaag aagtagcgga ttttgcagta
    86461 gataaactaa agctgacaga acaaaaagac gaacagctga gccaagctgt catcaacgaa
    86521 gctgagcaag cacatattcg atttgttccg actgtcatct tgggtaaaga aatctttgat
    86581 gaatctattt caatagagga attaaaagaa ttgatccaaa caaaataaaa aaataaggag
    86641 cgtaggacaa aagtaaagaa cacttttgtc tcacgctcta aacacctata aacggcggaa
    86701 gcagaagcaa ctccttctta tttcttggag ttaaacgctt ctgtcccagc ctccttattc
    86761 cgaataaatg gtgtccaaaa gcttgcatct gccagattat ctcatttctt atgacaagct
    86821 gagcaaatta tctccaccaa ataatttatt catatttttg acttgcgcat actaactaat
    86881 tcttaaattt tcatacaaaa tttctcaaat tcccttaacg agtaatattt atgtacggtg
    86941 attcacacta gtatcccaat cactttttaa gttatcccga tttcaattct acttcaaaaa
    87001 tagttgcttt tatgtatgct gttagttgat tagatggtcg tttgttgagc taggtcgttt
    87061 attttaaaca agttgtttct ttagcttata cctatctcgt ctaccaaacg caagatggca
    87121 aacacaaatg catacgcatc tgtatttgcc actttgattc gtataaagct aacaactcct
    87181 aactcctctc atttaatagt tgctctcttt tttattcaga tacatttttg ctgatcacag
    87241 cctagtattt caatggtttt gaacactaaa ccaaattgtt ccattacaac attcactggc
    87301 tgatgaaatg gattaataga tttcgattat tcaaagcgat tttctattgg tggattgtgt
    87361 tcccaactaa caagacggat tccaaagtca ttatataact cttcaagagt taaccctttc
    87421 ctgttcccaa tagatgtatc tctcccacgc tcgatataaa atatggagtt tcgaatgcca
    87481 agacctggca aatctttcct tgttacagtt acactgctac taacttcccc atataaaatc
    87541 cagctcctcc aaaatgataa tatatttttc gaccatcatc taactcatgt aacaataatt
    87601 gttgtgccct cttgtcactc gccaaatgca aacgccacgt gcgataccat gccatgctac
    87661 ctgcatctgc agaaaatcca tcaaataatg caacaattgt atcttctatc acaatccttt
    87721 ccccatctac ttcatattct aatctaaaat caaactcccc tcgggtaatc gcaggtcttg
    87781 gtggcttagg acccaatatt attctactta aaaccgcatt cgttcccaga tgaaacacag
    87841 ctataacaac aataatgatt aatattttgt ttctatttga taaatttctc attattcgca
    87901 aatcacctct acattctcac atactagttc gtaatactcc tttaatatca aaatatctac
    87961 tgatcgtaat aatattcgct atgaattcct actactcgta taagaaaggc caaatcatca
    88021 gcttttactt gctgatgatt tggcttgttt ctatttatcg taattgtttt ctaatcacca
    88081 aaattgttaa caatcggttc agatatttcc caattcacta gccttatatt atattgattt
    88141 aacaattctt cttcatttat tgtattttga tcaagcggac caaattgttc taataattct
    88201 ttgtcatgta aaatatccct tctagtaatg cctctttccc tttcaatgaa agaagcacta
    88261 ggaaaagtat gattataacc attcaaccta tctcccatgt aataattagc aggtcctgga
    88321 aaataaaata tttgcttatt atcactaact tctaacagca acactaaatc ttctccgctg
    88381 gctaaacgac ttgtccatct acgatatctc cctatgcctt cgttcattcc aaatccatca
    88441 aattcaacta ttaacgtgtc ttctatcaca tgtaattctt cattcaattc atactcaagt
    88501 ctaaaaggaa attctccata tgtaatctct ggcaatggtg gtgctggagt tctccaaata
    88561 aatacaataa ctgtgaatag aaaaaatcca acgatcgata taaatgttat gagacctgct
    88621 cgaagtaaaa cccgatataa taaaaatgct gtatttcgct ttgctatatc tagaaaaata
    88681 aaaagtactg caatcataat gatagtaaaa gttgtaaaaa aacgcatttt tgccctccct
    88741 ttataaacat cgattctcaa attctaaacc aaatcttggt agttcaagtt caggtataac
    88801 gtattccaac catacatctc aattactatt tatgaactta tgattatcac tgaataaatc
    88861 actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc gactagctaa
    88921 tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac cagaagtaag
    88981 aagagcagtt ctcaaggatg atcgagcagt tccttccagt tttttaatta atcaggccag
    89041 ttatagataa atacttgatc ttatctatat caggacttct atattaagca aaaatttatg
    89101 tacaaaacta aaatcttcat tcagttgtct ccccgctttt tatacgattg tattaaaaac
    89161 aatagaagcc tttccaaatt atgttactcc agtaaagatg gaattatagt ttgacggaag
    89221 aaaagcaagt ataactttta aaaaaaactg atacattcgc atctcctgct tcatgtatca
    89281 gttttttctt tggattaaac tgcttcttca cgcagttttt ccgcaaattc atttattttg
    89341 cgaattcggt gattaattcc cgattttgat atcgcacctg agggaatcat ttcccctaat
    89401 tctttcaagc tgacctctgg atgttgaagc cgaagttctg cgatttcctg aagtttttca
    89461 ggaagggcat gtagaccgac ctttgcttcg atcaattcga tgttttcgat ttgtttagaa
    89521 gccgcatcaa tcgtcttgtt cagatttgct gtctcacaat tgactagacg attgaccgaa
    89581 tttctcatat cccgtacgat tcgaacatct tcaaatcgga gcattgagtt ggttgcacca
    89641 atcaatgtta agaaatcagc aattttttct gctcctttta aataagaaat atagccattg
    89701 cgccgttcaa gcgttcgagc atgtagaccg taataattta acatatcgca aatgtcttgg
    89761 ttatgctgtt cataaataga aaatatttcc aaatgataac ggctcgtttc cggattattg
    89821 actgaacctg aggccataaa agcaccacgt aagtaagaac gcattttttg cgaatttccc
    89881 ataatctcgt ccgaaacgtg tccattgaac atcataccat ccatgatatc aagatccatc
    89941 aacacatctt ttgtgccttg cttcaaccgg acgatataga cattgttctt ttttaatttc
    90001 atttttttac gaacgagtag ctcacttcgt acctgataat gatctttcaa aagagaatag
    90061 atacgtctgg caattgcagc attttctgtt tggacgttca aaataaactg ctggttcaca
    90121 atacctaaag agccgttcat tcgcagcaaa gcagctaatt ccgctttcgc atgttctcga
    90181 tgaacttcca aaccagttag ttctttcttc acatctgcag caaatgacat cctattctcc
    90241 tcctttctcc aacaataaat tgcttaatat tttgaaccga aaactaaacg gaacaattct
    90301 tctactactt tgtctccatc gtggaagaca ccaccatctt tcaattccaa aaagtcggtg
    90361 gaaatcactc gacatccttc atttctcaat ccgttgaagt cgtgtgcgac ttgtactaga
    90421 tactcatcat agatctctgg gtccatataa tcttcaggca ctttttccgt attgaccaat
    90481 actgtatcga taaaaggctg attcaaatgc ttgtgtaaaa cacgtatatg atcggcatct
    90541 gtgaaatgtt ccgtttcccc tttttgggtc atgatattac agatatagac ggtttcagca
    90601 gttgtcttga gcattgcttc gccgatttct ggtatcacta aatttggaag gatactggtg
    90661 aataaacttc cgggtcccaa aacgaccatg tcggcgtcca taattgctga aacgactttt
    90721 cgagccgctt tggcttcact tttatcattt gtgttgcaga catacacacg gtcaatggtt
    90781 ttacggtcga tcgcgatttt agattctcct actgcagtcg ttccgtcttt aaagacagca
    90841 tgcaaggtca aaggagtttc tgaagaagga taaacatgtc catctacatg catcattttt
    90901 gctaataatt gaatcgcctc ataagtgctt ccccgcatct cggaaactgc ggcaatgatc
    90961 agattaccga tcgtatgatt ggctaaaaac tgatcttcct ttttgaaacg gtattggaaa
    91021 atatcttcat agaattgtgg catatccgat aacgccacta agacatttct caagtcacca
    91081 ggaggagcca tagcgatcga ttctctgatt gcaccgctgc ttcccccatc atctgcaaca
    91141 gtcactactg ctgtgatatc tgctccttga tttcgcaaac ttttcaggat aactggcaga
    91201 ccagtgccac cgccgaccac tacgatcttt ggttttctta tccggtaggt tttcattttc
    91261 acgaccggtt caccgtctct ttccttttat ccttgtcccg atgtgtcaca ttgactttat
    91321 agtttttccc taaagcattt gccaaacgtt cagtcaatgc cacagaacgg tgttgaccgc
    91381 ctgtacagcc gattgcaact gtcaagctgc tttttccttc tttaatgtat ccaggcaata
    91441 cagtttccag aagatccaaa aatttatggt agaatgattg cgtttcatca aaggacatca
    91501 cataatcata gactggctga tcttttccgg tcaatggtct tagttccggg atataatgag
    91561 ggttgggcaa aaaacgcaca tccatcacaa tatccgaatc gatcggcaac ccgtatttaa
    91621 aaccaaatga tacaaattcg atccgaaatc cttggtctcc ctttgatttg aatgactcat
    91681 tgattttttc acgtaattgt cgtggtgtca atgtcgtcgt atcaatgatc acggaagctt
    91741 gggctttcaa gtcgtctagt atcgcccgtt ctttacgtat cccttcagtg atcaaaccgt
    91801 ccatcgccat tggatgtgct cgtcttgttt ctttgtagcg agccactagt tcttcgtctg
    91861 aagcatctaa aaataagata gaagtgtcga tgaagttcgt attttcgatt tcgatcagca
    91921 tgttttggat ttcttcaaag aaggaacgag aacgtaaatc cactcctaaa gccatttttg
    91981 tgactttgcc tgattctttg attaattccc agaattttgg aatcaatcga ggcggcatat
    92041 tatcgataca aaaatagccc atatcttcaa aactttggat agctactgtt ttccctgcac
    92101 cgctcatacc tgttatgatg actaattgca aattatctgt cataattacg aactcctctc
    92161 acattgtgca tagtaaaatt atatcattcc gggcgtatca ttaccagttt tctttttctt
    92221 ttacaaaaag atttggacca aatatctttt aggatatttg ccccaaatcc aattttttta
    92281 cttttccgat gctgtatcca atgccttgct tctttcagct agcgctcgtt caatcgtccg
    92341 atctgctaaa atagattggg catcgatcac tggataatgg ttatcttcag caaattcatg
    92401 catcaatgac aattctgtgc aacctaagat aactttttca cagtttaggc gctctaccgc
    92461 ttcttctaag atttcgtaat atagctcttg attcaaataa tccgattcct tgatctcatg
    92521 atagatcaga taattgattt tttcttgaag aacagtatca ggaatcactg tttcgaatcc
    92581 aagatttttg acttcgcgtt catagattcc agctttcata cttccttctg ttcctagaat
    92641 cgctactctc cctgtcgtat gctgacggac aagttcattt gctgcttctc ttggcatatg
    92701 aaggatagga atatccgttg ccgcttgtaa ttcttcaaaa aagtagtgtg cggtgttaca
    92761 agtcaacaca atgaagttcg gctgcaataa attttgtttc tcaatatcat ccagcagaaa
    92821 cggcattgga ttttcttctg atcgatctaa aatataagct gtacgatcag gaactgttgc
    92881 atgattgaat aagacataat tcaaatattc ttgatctttt gtagctttcg ttcggtggtt
    92941 gatcaagcga acaaaacttt ctgtcgccat cgtgcccatt ccgcctaaaa tactgaaaaa
    93001 attctccatg aactagcctt ctttcacatg aaaatacttt ttgaatcttg gaatatagtt
    93061 atggaacata tatttcatca acaaccagcg cttgattgac cggtcttttt cataaaacac
    93121 agtagtaccg taacgatttt ctttgatcat ttgctcagct agttttttgt cttcattttc
    93181 ccgagcatat tctaaaaaga tttttttggg cacacccatc cataatcttg ctttatctga
    93241 ttgattcgtt ccataggtcg tttcaacaaa gggtttgtta aacactcgat cttctgtgac
    93301 gaaacgagcc agattcaagc cgttcaatgt cacaaagaaa ctgctacgac cttgtctcaa
    93361 attgatttca aataatttat attctccatc tctaggatca tacttcatat cgaaattggc
    93421 aaagcctgta tattcgattt tttccaaaaa tgccttaatg gtttgataga ttttttcgtt
    93481 ataatctggc atgatcacaa cgtaattacc aatcgatgct ggtgtaggat cttctaacag
    93541 tggatgacca agacacatca tccgaacttg atgatcttca tctacatacg catttagcac
    93601 acgcatatta ctatcatcac ccgggataaa atcttgtaca atcatttcac ttttataacc
    93661 ggcttcgtat atccggccta aaattaaatc gaactcttct ctagtctcta aaataaaagc
    93721 tttttttctt ccttcaaatt ggacagaaag atactccaca ctgtttgcag gtttcaaagc
    93781 aactgggaat tcaaaaggca attcctgttc caaatgtcca tttactaaca tttcttctct
    93841 gacgatcaat gttttaggat acggaagatc atattcttca caaacttcat aaaagcttac
    93901 tttattgatc agtctttcaa ataaagaata atcaatatat ggacagataa aatattctga
    93961 cagctcttgt ttatgttggg agatcaattc tgcatatcca tcaccacaag caatcaaaag
    94021 atatttttta ctcttatcag aatatctttc ttttgccaaa cgaagcatcg tctcgataaa
    94081 cacagggtct ttatcaaatc ctggaataac ttctacattg acaattttac tgtagcgggt
    94141 aggggctaac tgatccgaag catatgcttg gcagattttg ccgtatgctt cattaaaaga
    94201 acgtgccata ccataaacgt tcatgtcgct tcctaataat atagggataa attcttcatt
    94261 ttcaatactg ttcatcatta tctctcactt actcataatc tcttgttttt cttttccaac
    94321 tacacaagga atatcatacc ataaagaaaa aagcaatcaa aggaaactcc tccgattgct
    94381 caattctttt ttgaacggat tgaacttata ggaagttttt gtctatacca atcagtgtag
    94441 aaagaactac catgtaatga ttctagaaca ctgatattaa taaaacaagc aaaaattcat
    94501 ttttttatgt ttttttaact atctcagcta tctatcctct ttataccatt atttttttca
    94561 aatagtgacc tgtataactt tcttttacgt taatgatttc ttctggtgtt ccagtcgcaa
    94621 ggatcgttcc tccaccttct ccaccttctg gtcctaaatc aatgatataa tctgctgttt
    94681 tgattacatc tagattgtgt tcaatcacta aaactgtgtt accagcatct actaatcttt
    94741 gtaatacatg caacaagcgg gcgatgtcat cgctatgaag tcctgtcgtt ggttcatcta
    94801 gtatatagaa attctttcca ttagagattt tgtgcaattc actggcaagt ttcatccgtt
    94861 gtgcctcacc accggacaat gtcgttgctg gttgccccat cgtcacataa cctaagccaa
    94921 catcaacaat cgtttgcagt ttgcgatgaa tctttggaat gtgcttgaag aattctacag
    94981 catcttctac tgtcatttcc aaaatatcag caatgctttt tcctttgtaa tgcacttcta
    95041 atgtttcaga gttatatcgt ttgccatgac atacttcaca aggaacatag acatcaggca
    95101 aaaagtgcat ttcgatctta ataattccat ccccgcgaca agcttcacaa cgaccgcctt
    95161 ttacgttgaa actaaaacgc cctttcttat aaccccgcat ttttgcctcg ttcgtttgag
    95221 caaataaccc acggatatca tcaaatacac ttgtataagt cgctggatta cttctcggcg
    95281 tccggccgat tgggctttga tcgatatcga tgatcttttc gatactttcg tagccggaaa
    95341 ttgtcttgaa tttacctggc ttagcagaat tcttatttaa tttttgcgct aacgatttct
    95401 ttaagatact attgaccaat gtactctttc cagaaccaga cacacctgtg actgctacaa
    95461 attctcctaa tggaaattca acactaatat ttttcagatt gttttctgaa gcaccagtga
    95521 tcttgattgc tttcccatta cctttacggc gttcttttgg tacagggatt gattttttcc
    95581 ctgaaagata tttccctgtc aaagaatttt catcattagc tacttcttca ggagtgcctg
    95641 cagccacgat ttgtccgcct tgttctccgg ccccaggtcc gacgtcaatc agataatctg
    95701 ctgcccacat cgtatcttca tcatgttcga ctacaacgag ggtgttgcca agatcacgca
    95761 ttttctttaa tgagccaatc agtcgatcgt tgtcgcgttg atgcaatccg atcgatggtt
    95821 catctaaaat atacaaaact ccagaaagat tcgatccaat ttgtgtagcc aatctgattc
    95881 gctgcgcttc tcccccagat aatgttccgg aagcgcgact caaagttaga tagtctagtc
    95941 ccacattttc caagaatgaa agtctgtcat ttacttcttt taaaatcggt ttggcaatta
    96001 ctttctcttg ttcagataac gttaagtttg agaagaaatc tactgctttt tgaatagaca
    96061 actcatttgt ttgcccgata ttcgttccat tgatttgaac agatagagct tggggattca
    96121 atcgataacc tttacatgtc tgacaagtta attctgtcat ataaagacgc atttgctcac
    96181 gcgtgaaatc actgttcgta tcatgataac gacgctcgat attcttcaat accccttcga
    96241 aagtcgtttc tacatcgcga acgccaccaa aatcattttc ataatggaaa tggaaaagat
    96301 catctgtccc attcaagatg atatcctgat gctcttccgg taattcttcg aatggtgtat
    96361 ctaaatcgat accgaaactt tcacaagcct gcgctaacat ttgaggataa tactgagagc
    96421 tgattggatt ccatggaacg atagcacctt gactaagtgt cttcgttcta tctggaatca
    96481 ctaaatcttg atcgacttct aatttgacac ctaaaccatc acagtctgga caagctccaa
    96541 aaggcgcatt gaatgagaac agtcttggtt ctagttcgcc aacagtaaat ccgcaataag
    96601 gacaggcata gtgttcgcta aacaacattt cttcttcacc gatcacgtca accagagcat
    96661 agccgtctgc caatcgcaga gccgcctcta aagaatcaaa tagacgagaa cgaatccctt
    96721 ctttcacaat gatccggtcg atgacgatcg cgatatcatg ttttttgttt ttctccagct
    96781 caggcacttc acttaaatca tagatctcac catcgactcg aacgcggacg tatccctctt
    96841 tttggattcg ttcgaatatt tttttatgtt gtcctttttt ctttaccacg atcggtgcca
    96901 acacttggat ctttgtgcgc tcaggcaaag ccattacttg atctgccatt tgatctactg
    96961 attgactggt aatctcgata tgatcgttgg gacaaatcgg atgtcctaca cgtgcaaaca
    97021 acaagcgtaa atagtcattg atttccgtca cagtacctac tgtcgaccga ggatttttac
    97081 ttgttgtttt ctgatcaata gaaatcgccg gactcaatcc gtcgatgcta tctacgtccg
    97141 gcttatccat ttgtcctaaa aactgacgag cataagcaga aaggctctct acgtatctcc
    97201 gctgcccttc agcgtacagc gtatcaaatg ctagggaact ttttccagaa ccggacaaac
    97261 ctgtaacgac cacgaattta tctcgtggaa tcgttacatc tatatttttc aagttatggg
    97321 cacgtgcacc gtgaatcgta attttatcgt tagccatgtt gtcctcctac tttgaagctt
    97381 ttaattctaa aatcatatct cgcacattag cggctgtttc aaaatccaat gctttcgccg
    97441 catttttcat ttctttttct aatttcatca ataacgtatc tttttcttct ttcgtcattt
    97501 cttcatacga gacttgtacc gcttcttttg tatcatcttc cgattctttc gtaatagaaa
    97561 tcaaatctcg gatttcttta atgatcgttt ttggaacgat cccatgttct tcattgtact
    97621 tctgctgaat cgtacggcgt cttgatgttt catccatcgc tagacgcata gaatctgtta
    97681 ctttgtctgc gtacatgatt actttacctt cttcattccg agcagctcgt ccaatcgttt
    97741 ggactaagga acgttcacta cgtaaaaagc cttctttatc cgcatccaag atagcaacaa
    97801 gggaaacttc aggtacgtct aatccttcac gaagcaaatt gattccaact aacacatcaa
    97861 attcacctaa tcgaagatct cgaatgattt ctgttcgttc cagcgttttg atatcactat
    97921 gcaaatattt gactttgatc cctaattctt tgaaataatc tgtgaggtct tctgccattt
    97981 ttttcgtcaa tgtcgttaca aacacccgct ggtctttttc gactcgttca tttatttcgc
    98041 ctactagatc atcgatttgt cccatgattg gacgaacttc gatcactggg tcaagcagcc
    98101 ctgtcggacg gatgatttgt tggataacgg tatctgtttg ttcatgttca tatggtcctg
    98161 gcgtcgcaga gacataaatg atctgattga catgtttttc aaactcttct aaccgaagtg
    98221 gccgattgtc taaagcagat ggcaaacgga aaccgtaatc tactagcatc tgttttctcg
    98281 ctcggtcacc attatacatt cctctgactt gaggcatggt tacgtgcgat tcgtccgcca
    98341 cgatcaaaaa atcttcagga aagaaatcca atagcgtata aggcggttca ccttctttac
    98401 gtccgtccat atgccgagaa taattttcaa ttcccgacgt gtagcccatt tctaacatca
    98461 tttcaatatc gtaattggtt cgctgttcta aacgttgagc ttccagaagt ttgttttcat
    98521 ttcgcaagac agttaaacgc tgttctaatt ccgctttgat attcgctacg gcatgctcca
    98581 tatgttcatc attcgtcaca aagtgagtcg ccgggaagat tgccacgtgt tccgtttctc
    98641 ctaaaacttc acctgtcagt gcattgactt ctcggatacg ttcgacctca tctccaaaaa
    98701 actccacacg taaggcacgt tcatctcttg aagccggaaa aatctccacg acatcaccgc
    98761 gcactcgaaa acgaccgcgc tgaaaatcta tatcatttct ttcgaattgg atactgacta
    98821 agtcacgaat caactgattg cgatctagtt ctgctccctg tcgaatcgaa actacttgtt
    98881 tttggtactc aaaaggcgaa cctaatccaa aaatacatga aacggatgca atgacgatca
    98941 catcatttct ttctaataat gaactagtag ccgaatgtcg taatttgtcg atctcatcat
    99001 ttacacttga atctttttcg atatacgtat cgcttgaagg cacataagct tcaggctgat
    99061 aataatcata ataagaaaca aaatattcaa cagcattgtt agggaaaaat tccttgaatt
    99121 ccccgtacag ctgaccagcc aatgttttgt tatgggcaat aattagtgtt ggtttattca
    99181 ctttctcaat aagattggaa atcgtatatg ttttccctgt tccagtcgca cctaataaga
    99241 tttgtgcttt tttcccgccg actacgccat ccactaattg gttaatcgct tcaggctgat
    99301 caccagccgg ttggtatttc gagacaagct caaagtgtcg ggaggtatct ctctcaatca
    99361 ttcttcgtcc tccttttcat tcattccttt tcattttacg accaataaaa agaaaaggaa
    99421 agcaaaggca ttccttacta aaaaattata ccgcaccgaa catactttcg ccactttttt
    99481 atcttattat gcatgttacg ttttataaca taaaatgtta gaaactcgca tttttttcat
    99541 tttttcgagc ttacagtctt gtctgattca gcacttttcg cctataataa taaaatattg
    99601 gaaatttagg aggttgatta tgaaaagaaa gcatttattt cactcatttg ttttcttaat
    99661 gacaggactt gtgacacttg cgtttggaca tgttgcacaa gctgacaaca ctacaacggc
    99721 agagtcaagc acaagcgaga caatgcaaaa aatagagccc aaaaaagacg tatacgtaat
    99781 tgccagtgac tctgcttttg ctccatttga atttcaaaac gcaaagggcg agtacgaagg
    99841 catcgatgta aaattgatgg aaaacatcgc caaactgcaa ggatttaaca tccgaatgga
    99901 ctaccgtggt ttctctgctg ctttgcaagc tttggaatca ggacaagcag acggaatgat
    99961 tgccggaatg actgtaacag atgaacgaaa gaaaagctat gatttttctg attcttactt
   100021 tgatagcggg atccaattag ctgtcaaaaa aggagatact tcgatcaagt cttatgaaga
   100081 tttgaaagga aaaacagtcg gagcaaaagt tggtaccgaa agtgctgaat ttttaagcga
   100141 ccatgaatct gaatatggct attctatcaa atattttgat gctgctgatc aattgtacga
   100201 cgggttaaaa gttggcgcaa tcgatgcaat gatggatgat tatccggtaa tcggttatgc
   100261 gatcaaacaa ggacaagaat tggaaacacc gatcaaaaaa gaatctggcg gaaaatatgc
   100321 atttgccgtt aaaaaaggac aaaatcctga gcttcttaat atgttcaatg aaggattgaa
   100381 agaattaaaa agaacaggcg aatacgataa gattattaaa gaatatgtag cggatggttc
   100441 agaaacttcc agtgagtctt ccactgctga tgaatcaaca tttcttggac ttatccaaaa
   100501 caactatcaa gcattgttga aaggactttg gagaacaatt ctcctaactt tgatctcctt
   100561 tgctttggct atagtaatcg gtgtgatttt tggattattc agtgtagcac caatacgcgc
   100621 tcttcgcaca attgctagta tttatgtcga tatcatccga ggaattccaa tgatggttct
   100681 tgcgttcttt attttctttg ggttgcctgg tatcgttgga tttacgattc ctgacttcct
   100741 tgctggtgtt atcaccttga ctcttaacgc aagtgcttat atcgcagaga ttgttcgagg
   100801 cgggatcaac gccgttcctg tcggacaaat ggaagcttcc agaagtttag gtctttctta
   100861 taatcggaca atgcaaaaaa tcattttgcc gcaagcgata cgcatcatga tcccatcatt
   100921 cgtcaatcag ttcgttatct cattaaaaga cacaacgatc atttccgcaa tcggtgtagt
   100981 cgaattgctg caaactggta agatcattgt tgcacgtaca acacaaagta cgtatgttta
   101041 cttgatcatt gcgatcatgt acttgatttt gatcacagca ttgacaaaat tagcaaaagt
   101101 tttagaaaag aaggtgaagt aagatggcag aaaaaatctt agttgagcat ttagtcaaaa
   101161 aatacggcga taacacagta ttaaacgaca tcaatgtgtc catcaacgaa ggagatgtcg
   101221 tctgtatcat tggcccttca ggttcaggaa aaagtacatt tttgcgttgc ttgaatcgtt
   101281 tagaagaacc ttctgacggt gaaattatta ttgacggcgc ccacttgatg gataaaagca
   101341 cagatatcaa cttggtgaga caacatatcg gaatggtatt ccagcacttt aacctattcc
   101401 ctcatctttc tattttggaa aacatcattt tagcaccgat ggacctgaag aaagaatcta
   101461 gagaagaagc agagaaaaaa gcaatcaaat tgctgcagac agttggtcta gaagataaaa
   101521 aagatatgta tcctgaaatg ctttccggtg gacaaaagca gcgtgtggct attgctcgtg
   101581 ctctagcgat gaaccctgac atcatgcttt ttgatgagcc tacatctgca ctcgaccccg
   101641 aaatggtcgg tgatgtcttg aacgtcatga aaaagttagc agaacaaggt atgacgatgg
   101701 ttatcgttac gcacgaaatg ggcttcgcta aagaagtggc caatcgtgtc atgttcattg
   101761 acgacggaaa ctttttagaa gacggaacgc ctcaacaaat ctttgaaaat ccgcaaaatg
   101821 aacggacaaa ag
//
LOCUS       DAYYKC010000002        84948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000002 DAYYKC010000000
VERSION     DAYYKC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.002"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..535
                     /locus_tag="ACHBBW_000095"
     CDS             17..535
                     /locus_tag="ACHBBW_000095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9151813.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="ACHBBW_000096"
     CDS             663..1346
                     /locus_tag="ACHBBW_000096"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9151814.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="ACHBBW_000097"
     tRNA            1655..1728
                     /locus_tag="ACHBBW_000097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="ACHBBW_000098"
     CDS             1887..2246
                     /locus_tag="ACHBBW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9151815.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="ACHBBW_000099"
     CDS             2369..4000
                     /locus_tag="ACHBBW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9151816.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="ACHBBW_000100"
     CDS             complement(4062..4703)
                     /locus_tag="ACHBBW_000100"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9151817.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="ACHBBW_000101"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="ACHBBW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9151818.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="ACHBBW_000102"
     CDS             5320..5664
                     /locus_tag="ACHBBW_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9151819.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHBBW_000103"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="ACHBBW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9151820.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="ACHBBW_000104"
     CDS             6404..9211
                     /locus_tag="ACHBBW_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9151821.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="ACHBBW_000105"
     CDS             9402..9893
                     /locus_tag="ACHBBW_000105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9151822.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /locus_tag="ACHBBW_000106"
     CDS             9996..10985
                     /locus_tag="ACHBBW_000106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9151823.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="ACHBBW_000107"
     CDS             11018..11821
                     /locus_tag="ACHBBW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9151824.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="ACHBBW_000108"
     CDS             11842..12756
                     /locus_tag="ACHBBW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9151825.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="ACHBBW_000109"
     CDS             12839..13213
                     /locus_tag="ACHBBW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9151826.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="ACHBBW_000110"
     CDS             13352..13597
                     /locus_tag="ACHBBW_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9151827.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="ACHBBW_000111"
     CDS             13769..14833
                     /locus_tag="ACHBBW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9151828.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="ACHBBW_000112"
     CDS             15079..15504
                     /locus_tag="ACHBBW_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9151829.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="ACHBBW_000113"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="ACHBBW_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9151830.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="ACHBBW_000114"
     CDS             16043..16897
                     /locus_tag="ACHBBW_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9151831.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /gene="xseA"
                     /locus_tag="ACHBBW_000115"
     CDS             16898..18235
                     /gene="xseA"
                     /locus_tag="ACHBBW_000115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9151832.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="ACHBBW_000116"
     CDS             18240..18464
                     /locus_tag="ACHBBW_000116"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9151833.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="ACHBBW_000117"
     CDS             18466..19356
                     /locus_tag="ACHBBW_000117"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9151834.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="ACHBBW_000118"
     CDS             19365..20183
                     /locus_tag="ACHBBW_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9151835.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="ACHBBW_000119"
     CDS             20290..20739
                     /locus_tag="ACHBBW_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9151836.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="ACHBBW_000120"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="ACHBBW_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9151837.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="ACHBBW_000121"
     CDS             complement(22494..22610)
                     /locus_tag="ACHBBW_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9151838.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="ACHBBW_000122"
     CDS             complement(22683..23639)
                     /locus_tag="ACHBBW_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9151839.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="ACHBBW_000123"
     CDS             23810..24178
                     /locus_tag="ACHBBW_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9151840.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHBBW_000124"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="ACHBBW_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9151841.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHBBW_000125"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="ACHBBW_000125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9151842.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHBBW_000126"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="ACHBBW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9151843.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="ACHBBW_000127"
     CDS             26166..28358
                     /locus_tag="ACHBBW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9151844.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /gene="mraY"
                     /locus_tag="ACHBBW_000128"
     CDS             28384..29346
                     /gene="mraY"
                     /locus_tag="ACHBBW_000128"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9151845.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /gene="murD"
                     /locus_tag="ACHBBW_000129"
     CDS             29360..30721
                     /gene="murD"
                     /locus_tag="ACHBBW_000129"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9151846.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="ACHBBW_000130"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="ACHBBW_000130"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9151847.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="ACHBBW_000131"
     CDS             31850..33070
                     /locus_tag="ACHBBW_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9151848.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHBBW_000132"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="ACHBBW_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9151849.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHBBW_000133"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="ACHBBW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9151850.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="ACHBBW_000134"
     CDS             35832..36509
                     /locus_tag="ACHBBW_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9151851.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="ACHBBW_000135"
     CDS             36523..37122
                     /locus_tag="ACHBBW_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9151852.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37203..37424
                     /locus_tag="ACHBBW_000136"
     CDS             37203..37424
                     /locus_tag="ACHBBW_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9151853.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="ACHBBW_000137"
     CDS             37473..38255
                     /locus_tag="ACHBBW_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9151854.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="ACHBBW_000138"
     CDS             38276..38983
                     /locus_tag="ACHBBW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9151855.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="ACHBBW_000139"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="ACHBBW_000139"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9151856.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHBBW_000140"
     CDS             complement(42092..43618)
                     /gene="zwf"
                     /locus_tag="ACHBBW_000140"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9151857.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="ACHBBW_000141"
     CDS             complement(43633..44280)
                     /locus_tag="ACHBBW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9151858.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="ACHBBW_000142"
     CDS             complement(44464..44793)
                     /locus_tag="ACHBBW_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9151859.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHBBW_000143"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="ACHBBW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9151860.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="ACHBBW_000144"
     CDS             45714..46727
                     /locus_tag="ACHBBW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9151861.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="ACHBBW_000145"
     CDS             46727..48004
                     /locus_tag="ACHBBW_000145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9151862.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="ACHBBW_000146"
     CDS             48067..50769
                     /locus_tag="ACHBBW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9151863.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="ACHBBW_000147"
     CDS             50921..51238
                     /locus_tag="ACHBBW_000147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9151864.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="ACHBBW_000148"
     CDS             51268..51588
                     /locus_tag="ACHBBW_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9151865.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="ACHBBW_000149"
     CDS             51696..53156
                     /locus_tag="ACHBBW_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9151866.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="ACHBBW_000150"
     CDS             53224..53445
                     /locus_tag="ACHBBW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151867.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53658
                     /locus_tag="ACHBBW_000151"
     CDS             53476..53658
                     /locus_tag="ACHBBW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9151868.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53658..54071
                     /locus_tag="ACHBBW_000152"
     CDS             53658..54071
                     /locus_tag="ACHBBW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9151869.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54194..55375
                     /locus_tag="ACHBBW_000153"
     CDS             54194..55375
                     /locus_tag="ACHBBW_000153"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9151870.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55446..55610)
                     /locus_tag="ACHBBW_000154"
     CDS             complement(55446..55610)
                     /locus_tag="ACHBBW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151871.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55764..55837
                     /locus_tag="ACHBBW_000155"
     tRNA            55764..55837
                     /locus_tag="ACHBBW_000155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55798..55800,aa:Arg,seq:cct)
     gene            complement(55906..57045)
                     /locus_tag="ACHBBW_000156"
     CDS             complement(55906..57045)
                     /locus_tag="ACHBBW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9151872.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57344..57979)
                     /locus_tag="ACHBBW_000157"
     CDS             complement(57344..57979)
                     /locus_tag="ACHBBW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151873.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58092..58727)
                     /locus_tag="ACHBBW_000158"
     CDS             complement(58092..58727)
                     /locus_tag="ACHBBW_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM9151874.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58761..59222)
                     /locus_tag="ACHBBW_000159"
     CDS             complement(58761..59222)
                     /locus_tag="ACHBBW_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM9151875.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59352..59783)
                     /locus_tag="ACHBBW_000160"
     CDS             complement(59352..59783)
                     /locus_tag="ACHBBW_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9151876.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59801..60121)
                     /locus_tag="ACHBBW_000161"
     CDS             complement(59801..60121)
                     /locus_tag="ACHBBW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9151877.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60420..61196
                     /locus_tag="ACHBBW_000162"
     CDS             60420..61196
                     /locus_tag="ACHBBW_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9151878.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61211..61414
                     /locus_tag="ACHBBW_000163"
     CDS             61211..61414
                     /locus_tag="ACHBBW_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9151879.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61430..61768
                     /locus_tag="ACHBBW_000164"
     CDS             61430..61768
                     /locus_tag="ACHBBW_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM9151880.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61755..61934
                     /locus_tag="ACHBBW_000165"
     CDS             61755..61934
                     /locus_tag="ACHBBW_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151881.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61977..62447)
                     /locus_tag="ACHBBW_000166"
     CDS             complement(61977..62447)
                     /locus_tag="ACHBBW_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151882.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62534..63232
                     /locus_tag="ACHBBW_000167"
     CDS             62534..63232
                     /locus_tag="ACHBBW_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9151883.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63410..63751
                     /locus_tag="ACHBBW_000168"
     CDS             63410..63751
                     /locus_tag="ACHBBW_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151884.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63744..64415
                     /locus_tag="ACHBBW_000169"
     CDS             63744..64415
                     /locus_tag="ACHBBW_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9151885.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64421..65113
                     /locus_tag="ACHBBW_000170"
     CDS             64421..65113
                     /locus_tag="ACHBBW_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9151886.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65110..65859
                     /locus_tag="ACHBBW_000171"
     CDS             65110..65859
                     /locus_tag="ACHBBW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9151887.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65871..66140
                     /locus_tag="ACHBBW_000172"
     CDS             65871..66140
                     /locus_tag="ACHBBW_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151888.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66146..66310
                     /locus_tag="ACHBBW_000173"
     CDS             66146..66310
                     /locus_tag="ACHBBW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151889.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66303..66602
                     /locus_tag="ACHBBW_000174"
     CDS             66303..66602
                     /locus_tag="ACHBBW_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151890.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66602..66904
                     /locus_tag="ACHBBW_000175"
     CDS             66602..66904
                     /locus_tag="ACHBBW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9151891.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            66944..67309
                     /locus_tag="ACHBBW_000176"
     CDS             66944..67309
                     /locus_tag="ACHBBW_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151892.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67799..67939
                     /locus_tag="ACHBBW_000177"
     CDS             67799..67939
                     /locus_tag="ACHBBW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151893.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            67936..68118
                     /locus_tag="ACHBBW_000178"
     CDS             67936..68118
                     /locus_tag="ACHBBW_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151894.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68131..68325
                     /locus_tag="ACHBBW_000179"
     CDS             68131..68325
                     /locus_tag="ACHBBW_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151895.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68358..68570)
                     /locus_tag="ACHBBW_000180"
     CDS             complement(68358..68570)
                     /locus_tag="ACHBBW_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151896.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68698..68898
                     /locus_tag="ACHBBW_000181"
     CDS             68698..68898
                     /locus_tag="ACHBBW_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151897.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            68895..69167
                     /locus_tag="ACHBBW_000182"
     CDS             68895..69167
                     /locus_tag="ACHBBW_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM9151898.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69168..69353
                     /locus_tag="ACHBBW_000183"
     CDS             69168..69353
                     /locus_tag="ACHBBW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151899.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69331..69687
                     /locus_tag="ACHBBW_000184"
     CDS             69331..69687
                     /locus_tag="ACHBBW_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151900.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69647..70123
                     /locus_tag="ACHBBW_000185"
     CDS             69647..70123
                     /locus_tag="ACHBBW_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9151901.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70254..70982)
                     /locus_tag="ACHBBW_000186"
     CDS             complement(70254..70982)
                     /locus_tag="ACHBBW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM9151902.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            71030..71308
                     /locus_tag="ACHBBW_000187"
     CDS             71030..71308
                     /locus_tag="ACHBBW_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151903.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            71310..71696
                     /locus_tag="ACHBBW_000188"
     CDS             71310..71696
                     /locus_tag="ACHBBW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM9151904.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            71693..72073
                     /locus_tag="ACHBBW_000189"
     CDS             71693..72073
                     /locus_tag="ACHBBW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9151905.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            72185..72637
                     /locus_tag="ACHBBW_000190"
     CDS             72185..72637
                     /locus_tag="ACHBBW_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9151906.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            72634..74361
                     /locus_tag="ACHBBW_000191"
     CDS             72634..74361
                     /locus_tag="ACHBBW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9151907.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            74425..75612
                     /locus_tag="ACHBBW_000192"
     CDS             74425..75612
                     /locus_tag="ACHBBW_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9151908.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            75584..76153
                     /locus_tag="ACHBBW_000193"
     CDS             75584..76153
                     /locus_tag="ACHBBW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM9151909.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            76166..77530
                     /locus_tag="ACHBBW_000194"
     CDS             76166..77530
                     /locus_tag="ACHBBW_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9151910.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            77532..77810
                     /locus_tag="ACHBBW_000195"
     CDS             77532..77810
                     /locus_tag="ACHBBW_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9151911.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            77791..78117
                     /locus_tag="ACHBBW_000196"
     CDS             77791..78117
                     /locus_tag="ACHBBW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9151912.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            78107..78445
                     /locus_tag="ACHBBW_000197"
     CDS             78107..78445
                     /locus_tag="ACHBBW_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151913.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            78435..78800
                     /locus_tag="ACHBBW_000198"
     CDS             78435..78800
                     /locus_tag="ACHBBW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9151914.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            78807..79433
                     /locus_tag="ACHBBW_000199"
     CDS             78807..79433
                     /locus_tag="ACHBBW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9151915.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            79433..79897
                     /locus_tag="ACHBBW_000200"
     CDS             79433..79897
                     /locus_tag="ACHBBW_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151916.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            80092..82401
                     /locus_tag="ACHBBW_000201"
     CDS             80092..82401
                     /locus_tag="ACHBBW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9151917.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            82413..83117
                     /locus_tag="ACHBBW_000202"
     CDS             82413..83117
                     /locus_tag="ACHBBW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9151918.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            83114..>84948
                     /locus_tag="ACHBBW_000203"
     CDS             83114..>84948
                     /locus_tag="ACHBBW_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9151919.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        28620 a        13991 c        17851 g        24486 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagtttcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtaa taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53641 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53701 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53761 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    53821 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    53881 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    53941 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54001 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54061 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54121 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54181 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54241 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54301 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54361 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54421 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54481 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54541 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54601 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54661 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54721 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54781 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    54841 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    54901 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    54961 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55021 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55081 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55141 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55201 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55261 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55321 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55381 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55441 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55501 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55561 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55621 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55681 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55741 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55801 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    55861 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    55921 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    55981 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    56041 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    56101 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    56161 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    56221 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    56281 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    56341 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    56401 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    56461 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    56521 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    56581 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    56641 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    56701 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    56761 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    56821 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    56881 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    56941 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    57001 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    57061 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    57121 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    57181 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    57241 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    57301 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    57361 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    57421 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    57481 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    57541 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    57601 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    57661 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    57721 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    57781 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    57841 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    57901 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    57961 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    58021 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    58081 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    58141 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    58201 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    58261 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    58321 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    58381 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    58441 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    58501 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    58561 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    58621 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    58681 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    58741 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    58801 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
    58861 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
    58921 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
    58981 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
    59041 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
    59101 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
    59161 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
    59221 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
    59281 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    59341 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    59401 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    59461 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    59521 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    59581 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
    59641 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
    59701 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
    59761 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
    59821 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    59881 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
    59941 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
    60001 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
    60061 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
    60121 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
    60181 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
    60241 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
    60301 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
    60361 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
    60421 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
    60481 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
    60541 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
    60601 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
    60661 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
    60721 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
    60781 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
    60841 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
    60901 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
    60961 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
    61021 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
    61081 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
    61141 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
    61201 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    61261 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    61321 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    61381 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
    61441 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
    61501 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
    61561 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
    61621 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
    61681 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
    61741 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
    61801 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
    61861 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
    61921 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
    61981 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
    62041 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
    62101 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
    62161 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
    62221 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
    62281 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
    62341 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
    62401 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
    62461 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
    62521 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    62581 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
    62641 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
    62701 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
    62761 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
    62821 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
    62881 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
    62941 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
    63001 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    63061 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    63121 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
    63181 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
    63241 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
    63301 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    63361 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    63421 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
    63481 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
    63541 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
    63601 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
    63661 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
    63721 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
    63781 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
    63841 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    63901 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    63961 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    64021 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    64081 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    64141 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
    64201 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    64261 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    64321 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    64381 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    64441 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
    64501 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
    64561 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
    64621 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
    64681 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
    64741 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    64801 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
    64861 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
    64921 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
    64981 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
    65041 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
    65101 aggggaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
    65161 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
    65221 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
    65281 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
    65341 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
    65401 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
    65461 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
    65521 aaggctaagc ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag
    65581 cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg
    65641 tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt
    65701 tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga
    65761 agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg
    65821 ctagaacgag ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg
    65881 attatctaga actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc
    65941 tgagagacgt atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt
    66001 cgagttggag taaactcaga tactatttcg cactctatat cgatggaacg caattaagaa
    66061 atttagcata taccaaatta ctattcattg attgtgttaa aggcttgcaa aaacatctta
    66121 atgaacttga gcaggtgtaa acaagatgga cctaaagaca tttacagcac agatcgaatt
    66181 aatgcatcaa gaagctttaa gacaaagtgt gtcgtacgaa gacaagtggc tcaacacatt
    66241 tcatggcgga cgtgagagcg cacttgatca agtactcaaa ttactgaaag gagaatgtcg
    66301 ggatggataa gaaagcggca atgcagcgaa ttatcgaatt gacttattca gaagattggc
    66361 aggaagataa aaaaatagtt gcagaagtcc aaaagctcgg taaatcaatg tggactgaaa
    66421 agcccaaacg gagaacgccg agaaagattg caatctggca tggtgatcga attctagtaa
    66481 cgggtaccgc taaacagtta tctgaaatta ctggtctgag caaaaatatt atttgggata
    66541 gagctaggag cttatggatt gattcaaaag gaagacagtt taggtatgtg gaggagaaat
    66601 aatggacgaa ctaatcgcga aagtagagca gtgggcaaaa gataagggat tggatcaagc
    66661 ggatttcagc aagcaaatgt taaaaacggt cgaagaaatc ggggaagttg ccgcttctct
    66721 agctagaaaa gatgaacatg gtttaagaga cggaattggc gatgtagtag taaccttgat
    66781 catcttggca atgcaaaatg atatggattt atacgagtgc ttggattttg cttatgacga
    66841 aatcaaaggt cggactggaa agatggtcaa tggtgttttc gttaagtcca gtgacctgaa
    66901 ataattccat taatggaaga aataactgaa taggaggata gacatgattg atgcaaaaca
    66961 agtgcaaaaa cagaaagatg gaatgttgat gtttgaagca tatgttctac catttcttaa
    67021 tcaatttgaa gttttggaat gctctgccag cggagaagaa ttggagtatg ttgtaattcg
    67081 tgaaaccaaa gaaaacgttc agaagctaaa cgaatttctt tgcactatta actgttggga
    67141 tatgatagct ccaggatttt tatgtccagc tatgggagaa tttttggaat attgtcgtct
    67201 tgaagatgca ggcactttgg atttagctta tctggtatac aactacctaa acatcaacac
    67261 agatcatttg tggtttggca ctgccgaaag aaaatgggta gtccgctaac caccaatatc
    67321 aatgaaaaaa ggcggtttcc tgaaagtgga attgccgcca ttgctgtaag gttctttacg
    67381 cgactcaagg tcacagaaat ataaatgcaa atctcttttg aagtgatcca aagctctcgg
    67441 aaaatggaag ttttttgttt caaagaataa atgagatttg cgtcaagaac ctgagagtta
    67501 tttccaaaaa agcttccaaa gaaccgaatg agctaaggac atcatgtgta aacacataat
    67561 atagctctcc tttcacggct aatcataaca tgaaaaatat tcgatgtcag taggaaaaaa
    67621 ggtgaattat gaaaagaata ttaaattatc ccggcagtaa cagattctgc aattaaactc
    67681 cgaagctgat gtatcagtat caactgtaga atattatgag cgtggagaaa gtcaagcagt
    67741 cacaaatgta aaaatcgata acgtcaacgg tcttatctgt ttagatgaag gcgatagtat
    67801 gaatgaaatt gctgcagacc atgaattatc agaaactgct ttaattgatc aaatcgaatt
    67861 agctagacaa tactttgttc catatgcaga tacctggtcc aagcataaga atgacatcat
    67921 ttttccagag gaacaatgag agcaactgat ccagtaatta tccttgagga agccaaattt
    67981 atttggactc acgaagagat agagcaagca cgcttgctct tttctcaagg agttaagccg
    68041 agcaaagtag ctgaaataat gggtcaaaag attcttgatg tcggattgct tttgctccat
    68101 ctagcagaaa aaatttgatt tgaggtgaaa atgatgattc aacttgcagg catccaaaca
    68161 gggaaaattt atttttctgg agaaagcaaa agcgaagcca accaatggct attgaaaact
    68221 tacacgaaca ataagaagct aaggaaaaaa tatcctgatt tgttgctaaa ggatgatcag
    68281 attatgccgg aaccgatgat tttgacccgt aaggagggaa gttgaaacta aaaaaccagc
    68341 cgtgtgggct ggagttgcta tttcttttta ataatgagtt tttctaaatt taattcattc
    68401 tcacgagttt gcgtccaata atcagaataa tattttaatt gtttaagagt aggaatgtcg
    68461 tcttcggtaa aattagaaaa taattctgga gaattatctt tttttaattg ttctaatcct
    68521 gattctttaa aacgttgttc aacgagtttt actaaattgt tgatattcat gataaatcac
    68581 ctttttaaat ttaatttcga tttgattaat cgatacaagt attatatcaa ataaaaaagc
    68641 tgatttaggg ggtcttacaa aaacactaaa attttttcaa aaagaggagg aacggtggtg
    68701 aaagtcagag aacaattcaa gatagtgagc gacgaaggta atgtttacaa aataaagatc
    68761 gttaatatta atgaatatcg agaagcagat cacaaatatt tggttgactt atacatgaac
    68821 gataaatatt tgggagaagg attttgtgga gattcacttt ttgatgatga gcgaattttg
    68881 aaggaggaaa cagaatgatc cacgaactaa aaatactacc agaatatttt gaagcagtga
    68941 cgagcggacg caaacaattc gagatccgca agaatgatcg tgacttcaaa gttggtgatc
    69001 agttaatact acgtgaatat aaaaaataca ttaaacatga attacctgga ctaacgagac
    69061 caacatttag ttacactggt gactcataca aagcagagat cacttatatt actgattaca
    69121 tgcagaagtc cggatatgta gtgttgggga ttaaaggggt gaattaaatg aaagtataca
    69181 tcttagctat cacagagggc acatggatgt tcccagtcgg ttccggaaaa atctataaat
    69241 caaaaacagc tgcttataaa gcatttgaaa aatacaaaaa agaaaacggt ggcggcacta
    69301 acgcaaagat tctagtggct gataactggc atgaagaagg tgaacgtaat tgatttacct
    69361 gttcattttc gtggtactaa ctatttttat gctagtaatc ttattcaaaa aaagatgagt
    69421 aaagacgaac gaaaaattct ggccaacttt tatttagtat taaaaatcga acaaaatgtg
    69481 ttttcggaaa ggagaattgt atgggaaaaa caaaatcaaa gattaagaag aaaaaacgac
    69541 gtttggaaca aaaggcaatc cagaatggaa cggctaagaa aaaataaaaa agctacccct
    69601 ttcgagatag cagcgcaata ctattctacc ataaggggtg gcgtttgtga gatttcattg
    69661 gttaaaagat taccaagaac tagatgagca gattctttac ttaaagtgga atcttaataa
    69721 aagtaagctt gaattgaatc gatgggtctg tggtgattta gcaaacgtcc gcatcgaaaa
    69781 gaattcgaga acttcttctt tagaagagaa tatccaaaag atagaaaatg aattggaatt
    69841 gttgatcgag caaaaagaag aaatgttatt gctgatagat agcttttctg gtatcgataa
    69901 tcaaattgtt aagatgaaat atgttgatca aatgagctta gaagatattg ctgaatctgt
    69961 tgggtatagc tcatcgtatg tcagacagcg acatgcagaa attagaaaga cattgaactt
    70021 tttagatgag tatgaacaca gacaagctga ccgattgaaa aaagaaaacg aaattgattt
    70081 ttacaatagc gagaagtaca aagaacaact ctctttattc taaaattaca ctgttcacta
    70141 aatgttcgta aacattgtta gcagcatgat attctattag tgtcaaaaaa atatgaaaga
    70201 gccaagatat cccaaccgtt ttattaattg gtatctgtgg ctcttttatt cttttaatta
    70261 gtagttggca taaagttatt tccatttata aattgatcag ctaaatcagc atcataatca
    70321 gccgcgataa attgcgctgc tgatttaata aatgcatcca tatcatgaac atctttatta
    70381 gtatgtctac gaacatagtg cgtttcatca ttaccaatcc aagtagcagc tttggctaat
    70441 ctttggagtt taggaaaatc attcaaataa ttttcaattg ttttacctaa taacattttt
    70501 tttattttat cttcatcctc tgaattttta gatattgcat aatctttaat tagaaattct
    70561 agagcttttc tataaccaac tccagcaatt tgttccaatt gctcagattt agcttgtgtg
    70621 gcttgagtat aaatatcaac aaatgtagga gaaacttttt caatattttc tggtaaatct
    70681 attttaatcg gcggacggta ataatcttta attaaaatat tttcagaata tccttcatct
    70741 tcattaaacg tatttataaa ttgtaaacta taaaatttgt tgcactcatc atttatacac
    70801 tgtactaata cgccaaagat tctatcatta tcgaacgcac tatatgatga aaccccaaaa
    70861 tacacatacg gagtcattat atttccgcaa tgcggacaac tatttggcaa ataaattgtc
    70921 tctgtatttt gacgatcacg atacttaata ttaacttctt gaagcattaa aacacctcgc
    70981 atctttttta taaatcatac cacaaaatat ctgataggag agtaaacaaa tgaatgattt
    71041 tcatgaagcg gtacttagta ttgaagtaga atcaagtttg gctaaagttt ataagaaagc
    71101 aattgaagca gagaatagtc cgtacagaga aaattggaat ggtaatcatg cacatgtgca
    71161 agtggagaat gatgattaca gaactggaat gaatacgtta gttatttctt tattgtcaca
    71221 cactctaccc aatctgcagg aaacgattga atggtatgag cgaatgggcg caaaagtaat
    71281 ccgtacaaat tacaaaggag agaattaaaa tggcaatgat caaaattaga acatccatta
    71341 gtggaacaga gtattgggat tcagaaaaga aaaggacagt agttgttccg aaagatcaag
    71401 aaccaaattt tgaagtaaaa gaagaaaaag gaattttgag tgatggcaaa tcatttgttg
    71461 ctgtcaatgg cgaactcata gctcatagca gtgaaattct tgatagcgat ggaaacactg
    71521 ctgctaattt tgatggagac gaagctacta atgatcagtc cactgaagat acggatgaac
    71581 tggacaatat gactgcaaaa gaattgcgtg catatgctaa gaaacatggt attgatattc
    71641 ctggtgctat ccgtgcaaaa ggcgatatca taaatcttat tcgtgaagta gaatgaaata
    71701 ctgtcagttt gacggatgca cgaacaaaat agcaaagggt atctactgta ctgaacacaa
    71761 gagatcaagc agatcacgca agaagaagca gcaagcaaag tctgtttatc atcatgagaa
    71821 caaaccattc tatcgaacgc aagcatggaa agatatgcgt caatttattt atgaaagaga
    71881 aggtggtcat tgtcagcggt gtggtcagtt catatttgga aagagagcac acgtccatca
    71941 cattgtacca atcaaagaca acgaactgct taagcttgat ccaaacaatc tcatgctatt
    72001 atgttcaaaa tgtcatccaa ttgttgaaaa cgaaacggaa gacaaaaaag tttttccttc
    72061 gtatttcaat tgaagccccc ctattcattt tcaaaatttt ttcgcgtggg gagatagggt
    72121 agcggggagt cacgcgcatc gttaggtcaa atttttcaaa aaacaaaggg gggtgtatac
    72181 aaaaatgacg actaaagcgc aacgtaaagc gattattgat gaaaaagtaa gtgctgaaaa
    72241 agctcgtatc ttagaaataa tgaatttgtc tgatttgtac accatcactc ttgatccatt
    72301 aatcgaatca tacttggata tttttgaaat ttaccaacac aaatatttat tgtggaagga
    72361 aaaaggattt ccggagacgc aaaaattcac gaataaatca ggagctacta atcaatcaaa
    72421 acatccattg gcacaacaag tagaaacttg ggcagataag aagatgaaag ctctagattt
    72481 attaggatta acaaacaaag caaaaactgg tagactaatt gctggaggat cgaccgcaag
    72541 aaaagatgaa gaaattacac gcccagaagt aaagccagta gatgaactag cagcgcacag
    72601 aaataaatgg cgtaagaagg caggtgctga aaaatgattg aacctggtgt aaattatgct
    72661 gatttatttg caaaagaagt aagaaagaaa cctaagaagt atcctaagac cgttcgttta
    72721 gcaatagatc gctggtatca gtggaaaaaa agaaaagata tttggtttga tgtagatcgc
    72781 gcgaatgaaa tgatggattg ggtagaatca tttattgttc acacaaaagg agaaatggtg
    72841 ggaaaaccgt ttcttttaga accctgggaa aaatttattt attcttggat ttatggttgg
    72901 gttaaagaaa atgaaaaagg ccaaattgta cgcgttactc gtgaggctta tgtccagatt
    72961 ccaaaaaaga atggtaaaac attgattgct gtcggttcat taggatatgc aatgtacgga
    73021 gaaggggcgt tatcagttga ttgctatgct tgcgcatctg attttgctca agcgcaatat
    73081 gctgccaaac cttttgcagt tacaatctta aacaatccaa tcctacttga aggaactaaa
    73141 atatttaggg ggccaaaggg aactgtttct agtattactt atgactatat tcatgaagat
    73201 atggcttact caaataaatt tattgttcaa accaagaata ttgataatat cgagggatcc
    73261 aatccatatt ttgtattgaa cgatgaattg cataaacagg agaaaatgga acagtatgac
    73321 aattttaagt ctgctcagat atctttacca cagccattga tgtttaatat ctcaactgct
    73381 gggaaaggtt cgtcgtctgt tggaatgcgt gtatatcgcg aagcaaaaga agtgttgaaa
    73441 cgtgatgata atgattcaaa ctttgttcta atctatgagc caaataaaaa ttatgattgg
    73501 acggatagaa aagtctggga aatgtgcaat cctaactggg gaatatcagt cgatttatct
    73561 gctttagaat cggcatttaa aacagcgcaa cggtcagctc attccaaagc tgaatttcta
    73621 acgaaacact tggatgtatt tgtgaacggc gcggataatt tctttgaaca ggatcaagta
    73681 gagccatgtt tagttaccac acaagaactt ggtgatttaa gtggcgaacc atgttatatc
    73741 ggtttagatt tatcacgtac acgagatttg acctgtgtat ctttaaactt cccaacatgg
    73801 gatgaagatg gtaaagcggt tcttaaagta aagcaacttt attttattcc gaatgaagat
    73861 ttagagtttc gagaaaaaga agataatgtt ccttacagtg atttggctga acaaggtttc
    73921 gttgaatttt gcgacggaaa aatgattgat caagatcaga ttttgcagta catagaggac
    73981 tgcatgaatt tatatgatat tcagcaagtg aattatgatc cagcgatgag tgacaaactt
    74041 gttgagaaat tagaaaactt aggattggaa tgcgttgaag ttgctcaata tcctaaagta
    74101 ttgaatgctc cttttgacga tgtcgaacgg ttgttttatg agaaacgaat tcaatttgat
    74161 aatccattat tcctttattg cactttgaac gttgtagcaa ttactaacat caatggacaa
    74221 aaagcgccaa gtaaacgtca atcaaagaaa aagattgatg ggttcgtggc gtttttgtgt
    74281 ggtcataagg aaacgatgaa tcaaatgaca gatattgatt cagatgagtt agatgattac
    74341 ctaagttcca tctatagata aatagaaagg cggtgaaaaa aattgaaatt acgtgatcgg
    74401 ttatcgaatg cagtctattc ttttatggaa aaacgtggat atatcgagga tatttttgga
    74461 cactacacac gttatggtca aagatatgtg acggattcat ctatcatgga atcttctgat
    74521 gtttatgaat tagttcaaga catatctaat caggtggcat tggccacacc aattgttatt
    74581 ggtcccgacg gcaacgaagt caaagatcat cacttactaa atattttgaa gagtcccaat
    74641 gattatttga caggattcga atttaccaaa ttggaaacaa atactttatt gatcaatggt
    74701 gagacattcc cattaacgga tagggatcag cttcatttgg cgtacggtgt aacaactaaa
    74761 atcaatgaac gacttcaaga agaatttgaa atgaatggtc aaaaaatacc tggtcaaatg
    74821 attcgacaca ttaagaacat cggaactgat tcattaaaag gtgctggaat aattgatctt
    74881 gcaagaaaca ctctagaagg cgttctgagc gctgaaaaag ttttgacgga caaatatact
    74941 aaaggcggtt tacttgcgtt catgcttaaa ctagacgccc acatcaatcc aaataatagc
    75001 gcccaaacaa aaattgtcaa agctatattg gatcaactgg aaggaacgca aaatgagagt
    75061 gatcattctg ttaagatgat tcctttggga aaaggatatt ccatcgagac attaaaaagt
    75121 cctgttgatg acgcggcaat tttgaattat ttaggtgttt acaaaaaaga cttaggaaaa
    75181 tttctaggaa tcaatgttga tacgtatcaa tcgctgatga agacagatat tgaaaaagcg
    75241 atgatgtatc tgcacaacaa agcaatcaaa ccaatattga aaaacaagag cgaacattac
    75301 accgctcttt tttttatgcc taattctggc tatagagtgg aatggaaaat taatattttg
    75361 gattttgttc cttactcaac aaaaacaaat attggctaca acatcgttcg taccgggatt
    75421 acaagtccag ataacgtggc agaaatgctt ggttttccta aacagaatac tccagaaaca
    75481 caagctatct atatttcaaa tgacttatct aggattggcc agaaaaatgc aacagatgat
    75541 tccttaccaa cgaatgatca agacttgaaa ggaggtgatg gaaatgaaga agaaggaaat
    75601 tcgcacgatt gacatcacca atctttcaac acgttccgat gaagaaactc atacgaggac
    75661 cattagcggc tacgctgctg tattcaacag tcaaacactt ttatgggatg acttaagcga
    75721 agtgattgtt cctggtgcat tctcaaaggc aattagtaat tcggatgttc gttgtttgtt
    75781 caatcacgat tggtccaatg tgctaggacg tactaaaagc ggaacccttc gccttgaaga
    75841 agatgaacgt ggtttgaaat tcgaagttga tttaccggac acaacggtag caagggactt
    75901 agttaaatct atggaacgag gagacatcaa ccaatgtagt tttggttttg tgccgactga
    75961 agaaacgtgg gactacaatt ctgaaccaat gcttcgaaca atccatgaag tggaattata
    76021 cgaggtttct attgtgcctt tgccagcata cgaagataca gaagctgcat taagaagtcg
    76081 tgatgaatta gaaaaaaacg tcgaagaaag aaaaaaatta atcaaaaaaa ttaatcaagc
    76141 gctagaagcg taggaggaaa aatacatgga taaagaatta ttgaaaaaaa tgaaggcacg
    76201 tcgtgagcaa cgattgactg aattacgtga aaaagttgaa tcaggagaat tacgcgaagc
    76261 agatttagaa gccgtgaaag aagaaattga cagtgttatt gatgaattga acggaattaa
    76321 agacgaatta ggcgcagatt ctggaactga tgaaacagac gacaatacag atgatcaatc
    76381 gaatagtacg gattcagacg aaagccgttc tggtgaagac aacgatcaag aagaagattc
    76441 agacagtgaa gatagttcag aaaatcgttc tggaatgatt actcaacagc aacgagatgg
    76501 attacttgga tcaattaaga acggattgga ggcacgtgca aaaatgacca agaaacaaaa
    76561 agatcaacaa ctacgaaaag catttgctaa ttttgtagct ggaaatattt ctgaagcaga
    76621 agctcgagct ttagggattg aagctggcaa cggttcagtt actgtcccag aagtaattgc
    76681 atctgaagtt attacttatg ctcaagaaga aaatttactt cgtaaatacg gaacagtggt
    76741 gcgaacatca ggagatgtca aatatccaat tcttgtgaag aaagcagatg cgaatgtaaa
    76801 caagaaagag cgttcaactg atattgctga aacagctatt cagtttgatg aaattttgct
    76861 taatcctgcc gaattcgatg ctttggcaac agtaactaaa aaattattaa aaatgtctgg
    76921 tgttccagtt gaagatattg ttgtggaaga attgaaaaaa gcttatgtgc gtaaagaaat
    76981 caattatatg ttcaatggtg atgacgctgg aaatgaaaat cctggtgcat tagccaaaaa
    77041 ggctgtagca tttaaaaaac ctttagatct aactgcttca ggtgctgggc aaaaattata
    77101 tgatgcatta atcgaattta aaaatacacc agtgacagaa gtgatgaaaa agggacgctt
    77161 tattattaat cgagctgctt tgactgccat tgaaaaaatg aaaacagatg atggatttcc
    77221 tttgttgcgg ccattcacac aagcagaagg tggaattggt taccaattag ttggctatcc
    77281 tgtggattgg acagatgcag cagataaaaa gggtgaacca gacacgccag ttttatattt
    77341 tggcgatttt tctgcattta aaattcaaga agttattggt gccttggaaa ttcaaaaact
    77401 tgttgaaaaa ttctctggta aaaatcaaat tggattccaa atttacaact tgctagatgg
    77461 tcaattggtt tattctccat ttgaaccggc agtatatcgc tacgaaatta caaaaccagt
    77521 tggtggttaa gatggaagag caaactaaag aattgtcttt agaggaaaaa ttcaaatcac
    77581 atattcattt tgaagagggc atggatgatt ctttgctctc tttttattta aatatggcaa
    77641 aagattatgt caaaacagca actggtggcc aacaagaata tcttattttg atggttgccg
    77701 gcattgccta tgaatataga gtttcagagg atgaactcga caaagctatg aatgccatga
    77761 cgccatttat cgtgcaagga gcgattcaaa atgccgaaga gacagactaa taatctcaga
    77821 tggaaagccg aattgctaga catcaaaaca ggaacagacg gaaacgatcg tccaactaca
    77881 atttacgaat ttaagcgtct aatattctat gaagaactcg gtgtgacttc tcaagaaaaa
    77941 tatttatcac agcaagccaa gacagacgtt gtcagacgaa ttaaagtcag atgggataaa
    78001 tccatcacag agaaattaag tgcgctcaaa attgattctg taacgtataa cattactcgc
    78061 atttatacga atcccgatgc aagagaaatg gagttgagtt tagcttatgt cgattagctt
    78121 tgatgaattg aaaacagcgc tgaaatcaac aaagttagca gtgttcagag acaaagccag
    78181 attagggacg atgtatccat acattgtgta ctcaaatgtg agtaacagca aaaaaatggc
    78241 atccggtaaa gtctataaaa aattacctta ttaccaagtc tcttttttta cacttggaac
    78301 agaacaggat ttagttgtta ttgaacaagc gctgcaaaat gctggcattc catattcaga
    78361 ttttacaggt atacaaggtg atgagaacga cgataccgta accaactatt acacatatgt
    78421 gaggtgtgtg gaaaatgcca agtaataaga atggtttttc tgaaatatcg gattatttag
    78481 gaaacctttc aaaagttgat ccgaaaaaat tatcgttgga atctttagaa gaagcagcta
    78541 acttttatct aaagcagttg cttcctaata ttcctaagtc gctcttaaaa aagaaacata
    78601 tgcgcgatca aataaaagta gtagttgaaa acgatcaagt gaaagttcaa tttgaagaga
    78661 cggcctttta ctggcgcttt gccgaaaatg gcacaacaaa tcaacgtgcc caacattttg
    78721 caagtggtac gtatgaacaa aacaaagaaa aaattgaaga aattatgact agaaaaatac
    78781 ttgatttatg ggaagggtga ttttaattgg gaaaacaaga tactttttat tttgaaggat
    78841 tggacgacat tttaattgcg atgatggcaa cgccagattc tgttggaact gcaccgactt
    78901 atagtgaagt tttacgattg ccaattgcca caaaaatcgg tgtcaaaggg aatggaacag
    78961 cattagaaaa atgggcatcc agcaaaatgt ttcgtcgtgt gtcgcgagaa acaaaacatg
    79021 aacttgcgtt ggatcacgtg ggtattccga tagaagtgat ggatgaaatc aaaggattag
    79081 ttgctaaaag cggagtaacg tttagcaaaa ataccgcacg agaatttccg tacttcgcct
    79141 ttggatttat tggaaatatt gaaaatggcg gtaaaaaagc agtttggtat cctaatacac
    79201 aactatccaa tgtgattgac gaagagtatg ccactgcaga ggaagaaaca aaaattgacg
    79261 atgtaactgc aaatctggtt tccatcggac tgaaatacaa taatgtgatg tatgcaagtt
    79321 ttgattcaaa tagagacgga gcttctctag aattatttaa taaattcatt gctcaacctg
    79381 tatacgatga agaacagtgg aaaacattgg ctaaagttgg aggtgcgggt taatggctcg
    79441 gttatctgat tatggaattc acgttgaaga cttaaaaaat tctgctgttg taattattca
    79501 aggcgcagaa ttccctattt catttactat gcaaacgatg gaatttatag cagatgtgta
    79561 tggtggagat tattcgcaat ttgaatctga tatgaacgcc atgctatcta aaaaagaagg
    79621 acaaatttct tctgctaact tatcgcctag tgatctaaaa atcatgcgtg ccttgattta
    79681 tgccatgctg cgtactggtg gtttagagga agatccagaa actattttca aatttttggg
    79741 aatgagtgga gaggtgttgt ctgcttatag tacctgtatg gaaatttttg ctagccagac
    79801 atttcaggtg gaagacctaa aaaaatccaa gaagccacaa gactttcaaa aagcgcaagc
    79861 aaaaagaaag aaaaacaaaa agaatcggaa gagataggaa ctccttggag tttttatatt
    79921 tacgttgctc tcactctatt gaattggagt gagagtttct ttttaaagtc tacacctaac
    79981 ttgtggctca aatcttattt acagtggtta caacaaaaca ccgattttga accacctcaa
    80041 tctgtaacta tggataaatc gccttggtgg tagaaaggag cgctaagata aatggctggt
    80101 aaagaatctg atgtcgttct taattttaaa acgaatggcg aagtcagtta ttcgaaaaca
    80161 atcaaagaaa tcaacaaaga aatgaactta gccgctgccg agtacaaaaa ccaagtgtct
    80221 gcgatggaca aggatgcaac tcaaacagaa aaattgcgag cggctaaaca aaaattagaa
    80281 aaacaattag gattggctga acaacgatct caaatgctga gagaggaata tgaaaaatct
    80341 gtcaaagaaa ccggtgaata ttctgctgaa tcagaaaaac tttataaaca attactcaat
    80401 tctgaaaccg gagaaaataa acttcgtaca gcattagaac agacgaacga tgcacttaaa
    80461 gaacaaggtg acgtttctgt tgatacagca aaaaaactcc agaaaatcga agaaacaggt
    80521 gagaaagtaa aaggcgttgg tgaaaaaatg tctgttggag taaccgcgcc tattgtagcg
    80581 gcaggagcag caggacttgc agcatttggt gaagttgacg aggcacttga taccatcatt
    80641 acaaaaaccg gagcaacagg tgatcaagct gatagacttt cacagtcttt caaaaacgtt
    80701 ggttcaaata ctcatttacc tttacaaacg gttggggaag ctattggtga ggtaaataca
    80761 caatttggat tcatggataa aaaactggaa gattcaacca attatctcct acagtacgct
    80821 gaaatcaatg atacagatgt ttcgcaatca gcaatatctg ctcgacaagc tattgatgct
    80881 tatggactag aatatgatga tttgaattct gtccttgatg taacaacgaa aacatcgcag
    80941 aatactggtc aatctgtaga cgacttgatg caaaaagcaa ttgatggcgc accacaaatt
    81001 aaacaattag ggttgagctt tggtgaaggg gtcactttac ttggacaatt tgagcaatct
    81061 ggtgttgatt caagcgcagc tttaagtagt ttatctaaag caacagtggc ttatgcgaaa
    81121 gacggaaaat ctttaagtca aggactagaa gaactacaag acaaggtaaa aaatgcaggt
    81181 tctgaaacag aagccatcaa tgcagcagca gaagtattcg ggactaaagg cggtcctaga
    81241 atggccgatg caattcgtag aggtactttg aacctggaag atttagcaaa aactgctgga
    81301 gaaagtgggg gagctgtagg agacacattc gatgctactc tcgacccaat tgatcaagcc
    81361 gatcaagcta tgaacaatgc aaaattagca atggctgacg taggtgaagc agtccaagta
    81421 agccttttgc ctttttttga aaaagcaact tccttattac aggaattctc taaatggtgg
    81481 gggtctctag atcaagatac aaagaattgg atcataacaa ttgctggtat agcggcagca
    81541 gttggaccag tactaatcgt tcttggttca cttatgggat ctgttacaaa aatcgttggg
    81601 gaagttaaaa gttttatagg agtttggcaa ggacttgctg gtttatttgg aatgtcggga
    81661 ggctggtttg cattagcagt tatagctatt ggcgcattag ttactggact aatttgggca
    81721 tataataacg ttaaatggtt tcatgatgga gtgaattctt tcttcaaagg tgtttcagat
    81781 gtagctgttg aaatatttaa ttttttaagt ggttattttg gtggattttt cgatggtgtt
    81841 atagcaaatt tcaataattt ttataatgct ggaaaacgaa ttttcagtgg ttttctagat
    81901 tttatcactg gagtatttac agggaattgg tcaaaagcat ggcaaggtct agttgatatt
    81961 tttggaggta ttttcgatgg tattgttgct gtagggaaag ctcctattaa tgccatgatt
    82021 ggattgatca atggatttat tggtggattg aataatataa agataccaaa atgggttcct
    82081 ggtgtaggtg gaaaatcatt ttctatttca aaactgcctt atttagcaca aggcggtcat
    82141 ctaatcaatg gacaagcgat tgttggtgaa gctggacctg agttactgac tgccaagaat
    82201 ggtaaaacca cagttactcc attatcagac gaagaaaaac gcaaagggat cagtggaaga
    82261 gtttctggag gtagcattga acagcatata cacattggca aagtcgatgc aaacaatcca
    82321 tctgagttag atcgtatgaa tcgtaaattt gctaaagcaa acaaacaggc tattcgtgat
    82381 ttgggaggtg ttcctatttg agtcgacaat ttatgaatcc agatgaacca aattttattt
    82441 ggaaaaatcg gaatgcagta attgatatga attgcatcat tgaggcagag cttccagata
    82501 ttatgccgaa caagcgatac gaaacttaca cgattcaggg ccgaagcgga gaattgacgg
    82561 agacgtttga tgactatgaa ccttttgatt tggaaataga agggatcact attccacatt
    82621 cgaaactgag agaagtcaaa cgatggctca ctggtaaaag ccaattaatc acacataatg
    82681 atccagacaa gtatctagag gctatctgca gcatggataa agaagtccct ttcgaaaacg
    82741 agtggggctt cttttataca ttcgatgtta cttttcgttg tcagccactc aaaagaaaaa
    82801 tcggtgaagc acctaaaatt tttcattcta gtgttatgga tatttttgat catggtgacg
    82861 aaattgcaca tccctatctg gaaatagaat cgaacggagg agatataacg atcgctgtcg
    82921 gtgataaaag cctaacaata cttaatacgt tagctgcaac aatcgtggtt gatactcagc
    82981 ttggaaaagc aatacaagaa ggtttgaatt tatttacgcg tggtgattgg ccagtattgc
    83041 agcctgaatg gaatcaggta aaaatatccg gaaaatttaa agaagtgcgg ttttggaata
    83101 ggagtgtgta tctgtgacgc aagaatttat ttatgcttat aaaaaaatgc cagatgattt
    83161 gagcgtgaac ggagcgtcgt tagttgattg ggaagatttg ccagaaatca atcgtgtgct
    83221 gaatggccaa tatcgttttt atggtaacta ttcaagaagc ggacagtatc gctcttattt
    83281 aaaaaaagga aattttataa aagcgaaggt accagatgga tcatggcaat attttgaaat
    83341 ttataatgtc aaaaagaatt tgacctccgt ttctgtaaca gctcggcata ttggttttat
    83401 ggccaataag aactttattg tcaagtcgtt tacggataac ggcaatgggt ctcagatcat
    83461 gaccaatctt aaaaacagtt tggcatttga tcaaaaattt aactatcttt cgaatgtagg
    83521 gacaacgcac caatttacag caagacaagt cgcgccagtt gaagcaatca ttggttcaaa
    83581 taacggtaat caaaatttga ctggcgtgac tagcgcggaa cttgatatgg ataactatga
    83641 tttgaaactg gtaaaacaga ttggatcaga taatggtttt cggattgatt ttggcatcaa
    83701 tttagaagca atcgaagaag aaattgatga agaatcaatt gtcaatagtt tgtatttggt
    83761 tggcggtgtt cctgataacg attatgatga agacaaagaa cctatcgaat atggttattt
    83821 agaaattgat ggagtaacca acgagaatcg acgtattgcc aaacgtgaaa acggagattg
    83881 taaaacagtt gatgaattga tcaaatgggg caaaacctta tttgacaatg atcgtatcca
    83941 tgagccaaaa gctactcata cagtcagtat ggtggcgttg gaacacacac tcgaatacgg
    84001 tgacatgtat caagagctag cgtctttaca ctttggtgat gtcgctcatg ttagggcgaa
    84061 agagctagat atcgagataa aagagcgtat ggtcgaatac acttatttcc caacgcttgg
    84121 gaaatataag gatattgttt taggtaatga tttatcactg tacacttcgg cagtaaatac
    84181 tcaagcacaa gaattgaaaa agaaaatcga caatcgaaca gaaacgttag ttcaaaatgt
    84241 gttgaatgcg acggcatgga tcactggtaa ttctggtggg catgtcgttt ttcgtccaga
    84301 aaaagctcca tcagagattc ttatcatgga tactccaaat gtggcgaatg caaagcgagt
    84361 gtggcgttgg aatttaaatg gcttaggtta ttcagacaac ggagtaaatg gcccgttcgg
    84421 tattgctatc acatcaaaag gagaaattgt tgctgacttc atcaaagtag gaacaataaa
    84481 tgcagaagta ttcgaaactt cctttaatgc ttatggtgat gtgttaaaac ttgtaaaagg
    84541 cacgcttcaa atttggaatg aaaacaaaaa aatcatggag ctaaccaaaa aaggtatgga
    84601 attctggaat agaaaagaat ccattggaac gatcggaaca acagattcag caggaaatcc
    84661 atttccaaat gctgtaacac ctacacctct tgaggaaaat tctttggtaa tacgtacaaa
    84721 tggcgatgga aaatacattt taatttctcc aactgcagaa aaaggatttg tgttattagg
    84781 aaacggaaaa gcatattatt tcggtgattt agatattcag ggaaaactta ctgttagagg
    84841 taaagaagtg ataccaggac aaaatggcgg tccatctggc ggaggagaaa cacctggtgg
    84901 gtatcctgat gaattaaaaa cagatgcaga aaagagagct tggagaat
//
LOCUS       DAYYKC010000003        71207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000003 DAYYKC010000000
VERSION     DAYYKC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.003"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..413
                     /locus_tag="ACHBBW_000204"
     CDS             183..413
                     /locus_tag="ACHBBW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9151920.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            510..1811
                     /gene="murA"
                     /locus_tag="ACHBBW_000205"
     CDS             510..1811
                     /gene="murA"
                     /locus_tag="ACHBBW_000205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9151921.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1824..2000
                     /locus_tag="ACHBBW_000206"
     CDS             1824..2000
                     /locus_tag="ACHBBW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9151922.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2135..2431
                     /gene="yidD"
                     /locus_tag="ACHBBW_000207"
     CDS             2135..2431
                     /gene="yidD"
                     /locus_tag="ACHBBW_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9151923.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2616..3083
                     /locus_tag="ACHBBW_000208"
     CDS             2616..3083
                     /locus_tag="ACHBBW_000208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9151924.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3168..4052
                     /locus_tag="ACHBBW_000209"
     CDS             3168..4052
                     /locus_tag="ACHBBW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9151925.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4166..4402
                     /locus_tag="ACHBBW_000210"
     CDS             4166..4402
                     /locus_tag="ACHBBW_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9151926.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4490..4570
                     /locus_tag="ACHBBW_000211"
     tRNA            4490..4570
                     /locus_tag="ACHBBW_000211"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4524..4526,aa:Tyr,seq:gta)
     gene            4577..4648
                     /locus_tag="ACHBBW_000212"
     tRNA            4577..4648
                     /locus_tag="ACHBBW_000212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4609..4611,aa:Gln,seq:ttg)
     gene            4773..4943
                     /locus_tag="ACHBBW_000213"
     CDS             4773..4943
                     /locus_tag="ACHBBW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151927.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5163..5870
                     /locus_tag="ACHBBW_000214"
     CDS             5163..5870
                     /locus_tag="ACHBBW_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9151928.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5929..6573)
                     /locus_tag="ACHBBW_000215"
     CDS             complement(5929..6573)
                     /locus_tag="ACHBBW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9151929.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6699..6986
                     /locus_tag="ACHBBW_000216"
     CDS             6699..6986
                     /locus_tag="ACHBBW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9151930.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7094..7414
                     /locus_tag="ACHBBW_000217"
     CDS             7094..7414
                     /locus_tag="ACHBBW_000217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9151931.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7495..8808
                     /locus_tag="ACHBBW_000218"
     CDS             7495..8808
                     /locus_tag="ACHBBW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9151932.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8805..9524
                     /locus_tag="ACHBBW_000219"
     CDS             8805..9524
                     /locus_tag="ACHBBW_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9151933.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9652..10203
                     /gene="raiA"
                     /locus_tag="ACHBBW_000220"
     CDS             9652..10203
                     /gene="raiA"
                     /locus_tag="ACHBBW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9151934.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10425..12959
                     /gene="secA"
                     /locus_tag="ACHBBW_000221"
     CDS             10425..12959
                     /gene="secA"
                     /locus_tag="ACHBBW_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9151935.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13085..14186
                     /gene="prfB"
                     /locus_tag="ACHBBW_000222"
     CDS             join(13085..13156,13158..14186)
                     /gene="prfB"
                     /locus_tag="ACHBBW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9151936.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14291..14977
                     /gene="ftsE"
                     /locus_tag="ACHBBW_000223"
     CDS             14291..14977
                     /gene="ftsE"
                     /locus_tag="ACHBBW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9151937.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14970..15854
                     /gene="ftsX"
                     /locus_tag="ACHBBW_000224"
     CDS             14970..15854
                     /gene="ftsX"
                     /locus_tag="ACHBBW_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9151938.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16006..17454
                     /gene="cls"
                     /locus_tag="ACHBBW_000225"
     CDS             16006..17454
                     /gene="cls"
                     /locus_tag="ACHBBW_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9151939.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17791..18648
                     /locus_tag="ACHBBW_000226"
     CDS             17791..18648
                     /locus_tag="ACHBBW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9151940.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18763..19683
                     /gene="pstC"
                     /locus_tag="ACHBBW_000227"
     CDS             18763..19683
                     /gene="pstC"
                     /locus_tag="ACHBBW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9151941.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19683..20567
                     /gene="pstA"
                     /locus_tag="ACHBBW_000228"
     CDS             19683..20567
                     /gene="pstA"
                     /locus_tag="ACHBBW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9151942.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20582..21388
                     /gene="pstB"
                     /locus_tag="ACHBBW_000229"
     CDS             20582..21388
                     /gene="pstB"
                     /locus_tag="ACHBBW_000229"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9151943.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21404..22162
                     /gene="pstB"
                     /locus_tag="ACHBBW_000230"
     CDS             21404..22162
                     /gene="pstB"
                     /locus_tag="ACHBBW_000230"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9151944.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22175..22852
                     /gene="phoU"
                     /locus_tag="ACHBBW_000231"
     CDS             22175..22852
                     /gene="phoU"
                     /locus_tag="ACHBBW_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9151945.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23429..24121
                     /locus_tag="ACHBBW_000232"
     CDS             23429..24121
                     /locus_tag="ACHBBW_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9151946.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24118..24885
                     /locus_tag="ACHBBW_000233"
     CDS             24118..24885
                     /locus_tag="ACHBBW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9151947.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24985..26553
                     /gene="liaX"
                     /locus_tag="ACHBBW_000234"
     CDS             24985..26553
                     /gene="liaX"
                     /locus_tag="ACHBBW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9151948.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26850..27170
                     /locus_tag="ACHBBW_000235"
     CDS             26850..27170
                     /locus_tag="ACHBBW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9151949.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27170..27520
                     /locus_tag="ACHBBW_000236"
     CDS             27170..27520
                     /locus_tag="ACHBBW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9151950.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27687..28625
                     /gene="hprK"
                     /locus_tag="ACHBBW_000237"
     CDS             27687..28625
                     /gene="hprK"
                     /locus_tag="ACHBBW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9151951.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28640..29470
                     /gene="lgt"
                     /locus_tag="ACHBBW_000238"
     CDS             28640..29470
                     /gene="lgt"
                     /locus_tag="ACHBBW_000238"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9151952.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29514..30539
                     /locus_tag="ACHBBW_000239"
     CDS             29514..30539
                     /locus_tag="ACHBBW_000239"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9151953.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30554..31492
                     /gene="galU"
                     /locus_tag="ACHBBW_000240"
     CDS             30554..31492
                     /gene="galU"
                     /locus_tag="ACHBBW_000240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9151954.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31617..32057
                     /locus_tag="ACHBBW_000241"
     CDS             31617..32057
                     /locus_tag="ACHBBW_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9151955.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32060..32662
                     /locus_tag="ACHBBW_000242"
     CDS             32060..32662
                     /locus_tag="ACHBBW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9151956.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32721..33827)
                     /locus_tag="ACHBBW_000243"
     CDS             complement(32721..33827)
                     /locus_tag="ACHBBW_000243"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9151957.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34037..35038
                     /gene="ccpA"
                     /locus_tag="ACHBBW_000244"
     CDS             34037..35038
                     /gene="ccpA"
                     /locus_tag="ACHBBW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9151958.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35375..37720)
                     /locus_tag="ACHBBW_000245"
     CDS             complement(35375..37720)
                     /locus_tag="ACHBBW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9151959.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37999..38901
                     /locus_tag="ACHBBW_000246"
     CDS             37999..38901
                     /locus_tag="ACHBBW_000246"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9151960.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39114..40889
                     /locus_tag="ACHBBW_000247"
     CDS             39114..40889
                     /locus_tag="ACHBBW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9151961.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40955..42541)
                     /locus_tag="ACHBBW_000248"
     CDS             complement(40955..42541)
                     /locus_tag="ACHBBW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9151962.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42792..43016
                     /locus_tag="ACHBBW_000249"
     CDS             42792..43016
                     /locus_tag="ACHBBW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9151963.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43157..44908
                     /locus_tag="ACHBBW_000250"
     CDS             43157..44908
                     /locus_tag="ACHBBW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9151964.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44908..46692
                     /locus_tag="ACHBBW_000251"
     CDS             44908..46692
                     /locus_tag="ACHBBW_000251"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9151965.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47018..47869
                     /gene="licT"
                     /locus_tag="ACHBBW_000252"
     CDS             47018..47869
                     /gene="licT"
                     /locus_tag="ACHBBW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9151966.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47965..49845
                     /locus_tag="ACHBBW_000253"
     CDS             47965..49845
                     /locus_tag="ACHBBW_000253"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9151967.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49885..50787)
                     /locus_tag="ACHBBW_000254"
     CDS             complement(49885..50787)
                     /locus_tag="ACHBBW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9151968.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51018..52421
                     /locus_tag="ACHBBW_000255"
     CDS             51018..52421
                     /locus_tag="ACHBBW_000255"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9151969.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52421..53854
                     /locus_tag="ACHBBW_000256"
     CDS             52421..53854
                     /locus_tag="ACHBBW_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9151970.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            53999..54925
                     /gene="trxB"
                     /locus_tag="ACHBBW_000257"
     CDS             53999..54925
                     /gene="trxB"
                     /locus_tag="ACHBBW_000257"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9151971.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55139..56866
                     /locus_tag="ACHBBW_000258"
     CDS             55139..56866
                     /locus_tag="ACHBBW_000258"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9151972.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56881..57231
                     /locus_tag="ACHBBW_000259"
     CDS             56881..57231
                     /locus_tag="ACHBBW_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9151973.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57323..58183)
                     /locus_tag="ACHBBW_000260"
     CDS             complement(57323..58183)
                     /locus_tag="ACHBBW_000260"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9151974.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58275..58703
                     /locus_tag="ACHBBW_000261"
     CDS             58275..58703
                     /locus_tag="ACHBBW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9151975.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYETIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58865..59041
                     /gene="rpsU"
                     /locus_tag="ACHBBW_000262"
     CDS             58865..59041
                     /gene="rpsU"
                     /locus_tag="ACHBBW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9151976.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59068..59514
                     /locus_tag="ACHBBW_000263"
     CDS             59068..59514
                     /locus_tag="ACHBBW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9151977.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59668..61047)
                     /gene="brnQ"
                     /locus_tag="ACHBBW_000264"
     CDS             complement(59668..61047)
                     /gene="brnQ"
                     /locus_tag="ACHBBW_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9151978.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61753..62724
                     /locus_tag="ACHBBW_000265"
     CDS             61753..62724
                     /locus_tag="ACHBBW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9151979.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62748..64940
                     /locus_tag="ACHBBW_000266"
     CDS             62748..64940
                     /locus_tag="ACHBBW_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9151980.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64957..65433
                     /gene="ybeY"
                     /locus_tag="ACHBBW_000267"
     CDS             64957..65433
                     /gene="ybeY"
                     /locus_tag="ACHBBW_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9151981.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65417..65812
                     /locus_tag="ACHBBW_000268"
     CDS             65417..65812
                     /locus_tag="ACHBBW_000268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9151982.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65827..66726
                     /gene="era"
                     /locus_tag="ACHBBW_000269"
     CDS             65827..66726
                     /gene="era"
                     /locus_tag="ACHBBW_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9151983.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66869..67681
                     /gene="recO"
                     /locus_tag="ACHBBW_000270"
     CDS             66869..67681
                     /gene="recO"
                     /locus_tag="ACHBBW_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9151984.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68065..68982
                     /gene="glyQ"
                     /locus_tag="ACHBBW_000271"
     CDS             68065..68982
                     /gene="glyQ"
                     /locus_tag="ACHBBW_000271"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9151985.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68984..71059
                     /gene="glyS"
                     /locus_tag="ACHBBW_000272"
     CDS             68984..71059
                     /gene="glyS"
                     /locus_tag="ACHBBW_000272"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9151986.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23286 a        12469 c        15244 g        20208 t
ORIGIN      
        1 aataagggac tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt
       61 atttcctaaa aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat
      121 acattttatg gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca
      181 ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct
      241 acttagcctt ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata
      301 atacgcaaat tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta
      361 attttttctt agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa
      421 aaaaaggtac tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat
      481 gctcattgga attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc
      541 aattaaatgg aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg
      601 ctagtttgtt agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg
      661 tcttcactat gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa
      721 agaatcaagt aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg
      781 ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg
      841 caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa
      901 aaggatttca agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg
      961 cggatgaatt gatcggtaac acgatttatt tagatttccc tagtgtaggt gctactcaaa
     1021 atatcatgat ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg
     1081 aaccagaaat cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag
     1141 ctggtactga aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa
     1201 tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca
     1261 atgtgctgat tgcagatgct atctctgaac ataaccgccc attgatttct aaattgatcg
     1321 aaatgggtgc agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata
     1381 tcttacctac agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc
     1441 aaatgacagc tatccaatta gttgcagaag gtacaagtgt agtgactgaa acagtgtttg
     1501 aaaatcgttt ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg
     1561 gaaatgtagc aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag
     1621 acttgcgggc agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg
     1681 tgcgtaactt gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat
     1741 taggtgcaga cgttgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa
     1801 cgattgctta aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa
     1861 agattttaat ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat
     1921 atggtgtcat cggtggcggc aatccattta aagtatttca gccaagtttg tggattcata
     1981 ttcgagactt ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag
     2041 ccatgaagca gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc
     2101 tttttttagt aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt
     2161 tagaggatat cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac
     2221 ctgttccaat tatatgataa aagcaattca aacccatggc gcgctaaaag ggacggtaat
     2281 ggggactgca cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc
     2341 attgacattc cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt
     2401 tgtgaaaaat acgggtagca ctgagcgata aagaaacaat ttggaaaata gcttcttata
     2461 ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt gaacaccgtc aaaaaaaagg
     2521 ttgtgaaaaa tacggctagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt
     2581 aacaactccg caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag
     2641 atttaccatg gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg
     2701 tttccagagg gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct
     2761 atgtggcaag ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag
     2821 gaaaggggat tggcggaaaa ttacttgaga cagcttttca gaagctttca caactcgctt
     2881 ccagtcaaac tcagatcatc atacgaaccg gcagtactag cagtgcggcg ttacatctct
     2941 atcaaaaaat gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact
     3001 ctgagcccat ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact
     3061 tacctaaaaa tagctttaac taataaaatt tcaattcaag aaaatgcttt caaatgctaa
     3121 atagatacat tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag
     3181 tttttgattt tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag
     3241 gtaagggaca atttgcaacg cctgaatctt gggaagaaat gaaagcgcaa ttatttcaag
     3301 tttataaaaa aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag
     3361 tagtgaataa tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat
     3421 ttaatatttt ccgagatttg gaaactctgt ttcaacttcc agtaacgatt gaaaatgacg
     3481 ccaactgtgc cggaatggct gaattttatg aaggtgctgc ccgcgattat caagatgttg
     3541 cttttgttgt ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaatcaata
     3601 aaggggcaca tctttacggt ggagaatttg ggttgatgtt cttaaatggc gataagacat
     3661 tcagtaaact tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg
     3721 ataaaaatgc ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag
     3781 caaaagaaga agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat
     3841 tttcgtttga tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca
     3901 ttgatgagat caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg
     3961 aaccgcagat tttattatgt gaatacagaa acgatgctaa tcttgtaggt gcagcagcaa
     4021 actatctcgc tgtacgtcaa ggggagaatt aagagcttta gagaaggtgc agcttgcata
     4081 aaagaagtga atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt
     4141 atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg
     4201 tatagaagaa gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat
     4261 agggaaaaaa atgattggtg agatcttagc ctatggacca aaagaatatg aaattttctt
     4321 tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg
     4381 acactggaat ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa
     4441 atctggaaaa ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc
     4501 gaagtggcta aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca
     4561 ctctcctcca tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     4621 tgcgttggtt cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga
     4681 gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgatt taagatagag
     4741 ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag
     4801 cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa gtgaatattc
     4861 cgaaaaaagg cggaaaagcg gtccgggtaa aatcaaaacg cccaacgaat catacaaaaa
     4921 aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga
     4981 ttttagggtc ttttgttatt ttctaggttg gacatatcta aaaaattgtt tctgacaata
     5041 ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt
     5101 ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg
     5161 ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg
     5221 aacaaggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat
     5281 tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct
     5341 tagaaagaaa gatcggttcg ggtacatatg ttgcacgaga aaaagtacaa gaaaaaatgt
     5401 ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga
     5461 caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta
     5521 aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt
     5581 ttgaagttgc cagtgtgcca caaaagctga tcgatggcta cagcaaagca gagattactg
     5641 aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa
     5701 cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag
     5761 atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg
     5821 tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg
     5881 atccctgatt ccgtattaga aataaaagag aatcagaatc acctgttttt atatatgatc
     5941 agttaccagt tgctcgtgat aagaaacagg accctcttca aattggactt gtccgttcag
     6001 cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac
     6061 gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt
     6121 ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt gcaaggtacc
     6181 ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg ctcgtattaa
     6241 tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag ctacattgat
     6301 cagttgattt tttttgagga cttccaacat cggtatgccc gccgttccac ttggttctcc
     6361 atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc tgcagttatg
     6421 gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt cgtcctctga
     6481 ttctattcgt ctaagagaac agataaagcg tgattttttg atctcgatct cactttgtcc
     6541 attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt cattttcttt
     6601 attcagtata aataaagttc taggttgttt caacactgta acggttgcgc aattgctgta
     6661 gtatgcgata ataaagacaa aggcaggtgg atctggggat gcaggaacga atgaaaaagt
     6721 acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg ttgaccttta
     6781 cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca agggaaagta
     6841 cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc tggataaatg
     6901 caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc aataaattag
     6961 tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg acaaatttct
     7021 ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa
     7081 aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg gtatgagcac
     7141 aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg actctgatat
     7201 tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagacg tggatgtgtt
     7261 gttattaggc ccacaagttc gctttatgaa agctgatttt gagaaacgtc tggaaccaaa
     7321 aggcattcct ttagatgtca tcaatatggc tgattatggt atgatgaatg gcgaaaaagt
     7381 gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact atcgaacaag
     7441 cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa taatatgaat
     7501 catttttggg gtagaaggct tctgacgaga gatattaaag caatagactg tgaggaggta
     7561 aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgagcaactg gattctttgt
     7621 catcgctgtg gaatgagaac gtccgtcatt gatggacagc ttgcagatta cggttatttt
     7681 tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata tttgtttgat
     7741 cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct tactgaaaaa
     7801 caaacggaaa tctcaaagag aatactttgc cattcagaaa aaagacatca tcttatttgg
     7861 gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga aactttaaaa
     7921 gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga attgttttta
     7981 cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa tacagcaacg
     8041 gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg tttttatcgg
     8101 tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg ggatgcagga
     8161 ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta cttaagtgcc
     8221 acacctgata aaaagttact aaaggagatc aagcagacgt ttgacatcca caaacttgct
     8281 ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg ttttcatgaa taattggcga
     8341 caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca tcaattaatt
     8401 aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg tttgaaagcc
     8461 acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag agacaatcag
     8521 cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt aaccacgatg
     8581 atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg agcggatcat
     8641 ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg taaagggcct
     8701 tatacgaaca gtcaagtata ttttttctat gaagaaaaaa ctgcagctat tcgaaaagct
     8761 tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tactgtgagg tgcagttgct
     8821 gcagtaaaga tatcgtccaa aatttaacat tcagcgagat tattggtgtt tcgaaataca
     8881 gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa acaagaggct
     8941 gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag aaagtaaatt
     9001 ctatccccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg acctatgatt
     9061 ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc aatcaataca
     9121 agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg cagataaaaa
     9181 aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa
     9241 gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt cagatgctac
     9301 tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa agattggaga
     9361 tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa aaaatattga
     9421 ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat tgtttgcaga
     9481 tcgcacagcc gaaaatcatc cgaactattt ctttggcgag atgaattaga tgtggaaatt
     9541 tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta agaagaggga
     9601 aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt tatgtttaga
     9661 tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta cgttgagaaa
     9721 aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc gcatgtgaac
     9781 ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt accttatctt
     9841 gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt ggttgttgac
     9901 aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg
     9961 ggcatgaata ctgcaaatgc tgctgcttta ttcaatgaag aggacgaaaa cgaaaatgat
    10021 tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga tagtgaagaa
    10081 gcagtcttgc aaatgaacat gctgggacat aacttcttta tttttgaaga tgcagaaaca
    10141 aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat tgaaacagac
    10201 tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag ctgccgcaag
    10261 tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag ttcttacttt
    10321 aatacgctta agatatttca attatctata actaatgata aaatgaaaag taaagtctac
    10381 atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc aatttgattc
    10441 gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt gcaaaaaaag
    10501 ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag gcaaaaactg
    10561 ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt ccggaagctt
    10621 ttgcagtcgt ccgagaagca gcaaaacgtg tattaggttt atatccatac cacgtacaat
    10681 tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact ggtgaaggta
    10741 aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa ggggtacacg
    10801 tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc gaactttata
    10861 atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat gaaaaacgcg
    10921 aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt gactacttga
    10981 gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat tatgccattg
    11041 tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc atttcagggc
    11101 aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag
    11161 aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc gaagcaggga
    11221 tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa aatactgcat
    11281 tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta gatatcgatt
    11341 atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg atcatggatg
    11401 gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc gtagagatcg
    11461 aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt atgtacaaga
    11521 aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga gaaatttata
    11581 acatccaagt ataccagatt ccgacaaatc gtccggttat ccgtgatgat cgtccagatt
    11641 tgctttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc aaagatcgct
    11701 accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct gagttattat
    11761 cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa aatcacttta
    11821 aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca
    11881 tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt ggtggtctag
    11941 cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt cgaggacgtg
    12001 caggacgtca aggagaccct ggtatgtcac agttctactt gtcattggaa gatgatttga
    12061 tgaaacgatt cggttcagaa cgaatcaaat ctttcttaga tcggatgaag atcggtgagg
    12121 aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca caaaaacgtg
    12181 tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg
    12241 aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa aaaagcttat
    12301 ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat gcgcatacac
    12361 aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc agtactcttg
    12421 tccatgaaga tagtattact cttgcagata tcgaaggaaa aacagctcag gagatcaaag
    12481 agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa ctaaatggtc
    12541 cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga
    12601 cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt gcttatggac
    12661 agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa gatatggtag
    12721 gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt caaaacgtac
    12781 agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat gccaaagaaa
    12841 atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa caaggtcgga agaaatgatc
    12901 cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat gcaaactaat
    12961 ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc accttctttc
    13021 tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga aagaaggaaa
    13081 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
    13141 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
    13201 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
    13261 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
    13321 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agccatgcaa gaagaagcag
    13381 aaacacgtat caatgcgcta gatgaaaaaa tgaagacata tgaattatct atgttgttag
    13441 atggtcctta tgatcgaaac aacgccatcg ttgaattgca tccaggggct ggcggaacgg
    13501 agtcccaaga ttggggcagt atgcttttac gtatgtatac acgctgggca gagcagcatg
    13561 ggtatcaagt agagacacta gattatcaag caggtgatga agcaggaatc aagagtgtga
    13621 ccctactgat caaaggacac aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc
    13681 gccttgtacg tatctcgcct tttgactcag cgaaacgacg tcatacttct ttttgttcgg
    13741 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
    13801 aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa acagaatctg
    13861 ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca caacgttcac
    13921 aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat caattagaag
    13981 tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa atcggctggg
    14041 gttcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
    14101 acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta tttattgacg
    14161 gctatttaaa aatgcatcta caataagaac ccattgttaa attgaaataa aattgtaaga
    14221 ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
    14281 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc
    14341 tatccgcaat ctctcaattg tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc
    14401 tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
    14461 aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag ttccttatct
    14521 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
    14581 tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
    14641 agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct ttcctagcga
    14701 attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatagtga atacgcctaa
    14761 agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat gggaaatcat
    14821 gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta cacataacag
    14881 tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc gcattattcg
    14941 agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
    15001 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa
    15061 cgatcacatt gactttagtc ggtgtattca tggcagttat catgaacgca actaaattag
    15121 ctcaagatat agaaggaaac gttgatgtct ctgtattcgt agatatcggt acaaaagacg
    15181 aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa aaagttgaat
    15241 tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat
    15301 tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct actgatcctt
    15361 cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct cgttctgatt
    15421 acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg acatggggat
    15481 tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac acgattcgga
    15541 tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg
    15601 gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc ggtgcgattc
    15661 ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc aatccatcat
    15721 tattacgttc ccattattca ttgattcatc cacaaaatat cgtttggaaa atcaatcttt
    15781 taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct atgagacgtt
    15841 tcttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt ttcgttccag
    15901 ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct ttactttaaa
    15961 aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa aattatcgtt
    16021 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat catcgttttt
    16081 cgagaaagaa aggaaactgc tcaaacatgg gcatgggtgc tcgttttgat gttcattcca
    16141 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
    16201 gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga caaacaggca
    16261 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
    16321 tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat cactttattt
    16381 acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc agaagaccat
    16441 atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt tcgagatgca
    16501 ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc
    16561 acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga agtcgcattt
    16621 ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa tcatcgtaaa
    16681 atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaatgaatat
    16741 ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat ttacggagaa
    16801 gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
    16861 gtaggctatt ctcccaaatt ttttccgtcc attcactcta ctggtgatat agctgttcaa
    16921 atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta tttgaaaatg
    16981 atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc ggatggatcg
    17041 attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat ccaaatacct
    17101 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctaatc
    17161 gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa gacgatgatt
    17221 atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc atttcgcttg
    17281 gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct
    17341 ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga aaaccgagga
    17401 ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct ttaaaacata
    17461 aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa cggggccatt
    17521 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
    17581 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
    17641 ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca attatgagaa
    17701 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata
    17761 aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt cttaccaata
    17821 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
    17881 acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga aacttaccag
    17941 acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tggtttgtct
    18001 caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga agaaaaatcg
    18061 gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat cactcctatt
    18121 gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa actatttaca
    18181 ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt tatattgaac
    18241 cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga cggagaaaca
    18301 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt tgccgacaca
    18361 cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga tacgttgaat
    18421 gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac gatttgggca
    18481 tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt tttagactat
    18541 atcttatctg atgaagtaca agataacatc gttggagaac taggttatat ccctgtttct
    18601 catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc tttacacaaa
    18661 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
    18721 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
    18781 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt catcagtttt
    18841 ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt agctagtaaa
    18901 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
    18961 tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt gcctatgatc
    19021 acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt tgccattggt
    19081 gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt acagccggtc
    19141 atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt atcagttatc
    19201 gtgccttttg tcagaagtat ttttggcggg actgggtttg gtatcttagc tggtacattt
    19261 gtgttgttcg ttatgatctt gccgactatc acaacgatga ctgttgatgc attaaaggca
    19321 gtgccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg gcaaacaatc
    19381 tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt ttttggtatg
    19441 gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc agcattgatg
    19501 ccaacaggtc ttactactcc tgcttctaca ttgacgtcca tcttaacaat gggaatcgga
    19561 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
    19621 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
    19681 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata gtttcgggga
    19741 tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga ataccgcaca
    19801 tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggcggt ggaatcggga
    19861 tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt tttcctattt
    19921 ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta acagatgtga
    19981 ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt
    20041 tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt gcattggctt
    20101 tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta caagcggtcc
    20161 attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa acagtgataa
    20221 aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta agttctggaa
    20281 gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca cctgcgttag
    20341 attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc cgtcaagctg
    20401 aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct gatggtgttt
    20461 ctgtttcggc tggcgcttct gccgtgttga ttattgctgt tttactgttc aacttcggcg
    20521 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagcaa
    20581 aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt
    20641 agcactatat acggatgatc tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt
    20701 ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg gctgcggaaa
    20761 atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata cgaaaatcac
    20821 tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg tttatgagat
    20881 gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt cgatttatga
    20941 caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat
    21001 cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa
    21061 aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc
    21121 gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc ctatttcgac
    21181 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca tcatcgtgac
    21241 gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct atttaggaaa
    21301 agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc aggcaaccga
    21361 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagagattat
    21421 ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag ggatcgatct
    21481 aagcttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg gaaaatccac
    21541 ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga tcaccggaag
    21601 tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg aactaagaaa
    21661 agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct atgaaaatgt
    21721 catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc aagtagttga
    21781 agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc
    21841 tttatcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa
    21901 tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt ctactgggaa
    21961 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    22021 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    22081 cgaatacaat gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    22141 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    22201 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgccgtgca
    22261 caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag ttatcgaaaa
    22321 cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg aaatgatcgc
    22381 attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga aagctagctc
    22441 ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa
    22501 agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attatgtgaa
    22561 aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac
    22621 gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaacaat
    22681 caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact tgaatgttgc
    22741 cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    22801 ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct aaactgaagc
    22861 tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg
    22921 ttctgcatca agtaataaga acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg
    22981 ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgggaa agggtttgtg
    23041 acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa
    23101 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
    23161 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
    23221 tcaattgatt acaggaaaac tgatgaacac ggatcaaaaa ggagaatcga tttatttatc
    23281 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
    23341 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
    23401 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggtggaaaac agggtctatt
    23461 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
    23521 taaaacagaa gggatttctc tattaaatat atctatcctg ttcgtcgtta tagggatgat
    23581 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
    23641 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
    23701 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
    23761 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
    23821 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
    23881 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
    23941 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt cgttcaaaga
    24001 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
    24061 agtattatcg atccctatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
    24121 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
    24181 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
    24241 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
    24301 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
    24361 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagca
    24421 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
    24481 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
    24541 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
    24601 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
    24661 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
    24721 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
    24781 atggctattc ttaccacatt gtccgcacta cttgttcttc ctttaacctg cgtgactaca
    24841 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
    24901 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
    24961 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
    25021 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
    25081 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
    25141 caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
    25201 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
    25261 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
    25321 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
    25381 ttaggcgagt tgacagaaga agaattagca cagcgtcaga cgttagaagc agaaatcaaa
    25441 gaattagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
    25501 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
    25561 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
    25621 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
    25681 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
    25741 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
    25801 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
    25861 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
    25921 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
    25981 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
    26041 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
    26101 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
    26161 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
    26221 acatttggtg cgacagcaga aaacctttct gtgtctttgg taaatggtga tgtacgtctt
    26281 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
    26341 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
    26401 aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
    26461 ttgcaattcc gtcgtgtttc agatggtgaa atcgcgcaag tgcaactttc aacgacaaca
    26521 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
    26581 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
    26641 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
    26701 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
    26761 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
    26821 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
    26881 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
    26941 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
    27001 tatacatttt attagctttg attatcccat ttgcgcgttc tgcacgaaca aactatggac
    27061 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
    27121 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
    27181 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
    27241 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
    27301 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
    27361 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
    27421 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
    27481 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
    27541 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
    27601 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
    27661 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
    27721 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
    27781 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
    27841 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
    27901 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
    27961 gaggattacc agctccagaa gaaatgatcc aggcagcaaa taaaaatggg atagctgtac
    28021 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
    28081 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
    28141 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
    28201 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
    28261 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
    28321 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
    28381 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
    28441 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
    28501 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
    28561 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
    28621 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
    28681 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
    28741 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
    28801 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
    28861 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
    28921 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
    28981 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
    29041 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
    29101 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
    29161 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
    29221 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
    29281 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
    29341 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
    29401 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
    29461 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
    29521 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
    29581 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
    29641 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
    29701 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
    29761 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
    29821 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
    29881 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
    29941 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
    30001 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
    30061 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
    30121 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
    30181 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
    30241 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
    30301 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
    30361 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
    30421 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
    30481 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
    30541 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
    30601 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
    30661 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
    30721 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
    30781 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
    30841 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
    30901 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
    30961 agtaccattg acacagcaat taatcgatga ttacgaacgt actcatgctt ctacgatcgc
    31021 cgttatgcaa gttcctcatg atgaaacttc aaaatacgga attatcaatc caggagaagt
    31081 attggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
    31141 accaagtgat ttagctatta ttggacgtta cttattgact ccagaaattt ttgatgtatt
    31201 gtccgagcaa caaccaggag ccggaaatga aatccaacta actgacgcaa tcgatacatt
    31261 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttacg atgtcggtga
    31321 caaatttggt tttatgaaaa caagtattga atatggtcta acacatccag aagtaggtgc
    31381 acctttacgt gagtacatcc tgtctttagg taaaacatta gaagcagaag gaagcaagat
    31441 cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aacccttttt
    31501 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
    31561 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
    31621 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttta
    31681 tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag actgtagcag
    31741 aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct agacaagtag
    31801 aaggcttact ggtcaaaagt aatgaactgc tcttggatat caatggaaaa gtcgcaacga
    31861 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcagaa cttaatcatt
    31921 ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg
    31981 ttgccggaaa agtcggtggg acagcgatga aattttttaa aaacaaaaaa catactgaaa
    32041 cgacaggagg aaaataaaaa tggctaaaaa aggtggattt ttattaggtg cagtagtcgg
    32101 aggcacagcc gcagcaatcg ccgcgttatt gttagcacca aaatctggta aagaacttcg
    32161 ggatgaatta gctgcccaag cggatgattt gatggatatg gcttcagatt acacagatat
    32221 ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc aagatctttc
    32281 tcaacaagca ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat cagcagatga
    32341 agcaggaagt gaatctgacg atatcttgtt aaatcttaaa gaacaatcag ctgacttatc
    32401 taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat taggaagtat
    32461 tgctaaagat acttcagaag atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg
    32521 agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgact caaaagaaat
    32581 gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag atacaatgac
    32641 agattcatct acaaatcact aaaatcacaa atttatagaa taaaaaggtg ggagcggact
    32701 agctctcacc ttttttactc ttatttttct aaaacaagta attcttttgg tgtagtcgtg
    32761 aatggcacac agccatcgct tgtcacatat acacagtctt cgatacgtac ccctactttt
    32821 tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat tactaagtca
    32881 tttccttcta caagggaagg gaattcgtgg acggttgttc cgatcccgtg acctaaacgg
    32941 tgattgaaat attcgccgta gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt
    33001 tcacctgctg tcacacctgg acggacggct tcttgtgcgg caagctgtgc ttcaagagtg
    33061 atgttgtaga ttttttcttg gaagtcggtt ggtttttgat aagcaattgt acgagtagca
    33121 tcactgcaat agccattcca aacaacgcca agatcgaata acacaagttc attcggtgaa
    33181 atcttcgtat ttcctggtgt accatgaggg cttgcagcat ttgctcctgc aaggactaat
    33241 gtgtcgaaag acatttgtga aacaccttgt tttttcaatt ggtattcaat ttctgcaatg
    33301 atcgcttgtt cagtcacgcc ttctttcact gctttaaagc caatctcaaa tgcaacatcg
    33361 gcccatttac cagcttcgat cagtttgtct ttttcctcta gagttttgat cagttgtaga
    33421 cgttggatga tggaagttag atcagtgtaa gaatccgttc ccgtcaagat atcagagata
    33481 gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc ctacattgcc aggctgacca
    33541 tagcgagcac gaagttcttt tccgatgatc tcccaagggt tttcgctgtc taaatagcca
    33601 cggacatcat acgcccaact actgttttga gcatcttcaa cttccaaagc aggagtgaaa
    33661 aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcatg aggatcactt
    33721 tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa ttcgacttgg
    33781 tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg gcaacctact
    33841 ttctaaatta attttgtatt caataattgt accactgatt tattaaaaga ccaacgttct
    33901 aaaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg caattcttga
    33961 gattatttga tattctattt tagaaatgta agacgtattt tcagaaaaca tataaaatat
    34021 gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag cgagagaggc
    34081 aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc
    34141 aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa acgcagttgc
    34201 gcgtggcttg gcaagtaaga aaacaacaac agttggtgta atcatccctg atgtcagcaa
    34261 tatgttcttt gcttctttag cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa
    34321 tattatctta gcaaattcag atggcaacga ccaaaaagaa gtcaatgtat tgaataactt
    34381 actggctaag caagtggacg gcgtgatctt catgggtcac catatcacag acgaaatccg
    34441 tggagaattt tcacgttcaa aaactcctgt tgtattagct ggctctattg atccagatga
    34501 acaagtaggt agcgtaaaca ttgattatgt atccgcaaca aaagatgcag taaacaaatt
    34561 ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa
    34621 tggacaaaat cgtctgaaag gctataaaga agcattaagc gaaaataacc taccatatag
    34681 cgaaggattg atctttgaag cacaatacaa cttcaaagat ggtctatctc tagtcgatcg
    34741 gatccataat agcggtgcaa cagctgtcta tgtttcagat gatgaattag ccatcggtat
    34801 tttggatggt ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag
    34861 taacaattca ctactgacag aagtagctcg tcctcgttta acaagtatca cacaaccatt
    34921 atacgattta ggggcggtat ctatgcgcct attgacgaaa atgatgaata aagaagaagt
    34981 cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcta caaaataata
    35041 aaaggacaac cattcagagg ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat
    35101 ttcaaaaaac agcatctcat gtttgatgaa tattgtgact cgtgattcgg agttggcagg
    35161 agaacaccgt ttatttggga aaaggggtta tgacaaaatt ttgttataat cctttttttg
    35221 tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat cgtatataaa acaatccgtg
    35281 tatagatgct gcattttgtt caccaccatc aaaaaaataa gggactgtga caatactatt
    35341 gtcacaatcc cttattactg tgtattctat taaatcaatc atctttctca tctgattctt
    35401 tagttgcttg attcgctctt gcataagctg aagcggtgtt ccagtaatct gtatagtttt
    35461 catcggtacc gccaacgcca aatttaaagg cgcttttctt cggtccattt ttcgcccata
    35521 gcgattcaac tgtttttcct ggtgtttgga tggatttttt attgacagta acttttccag
    35581 gtgtttgacc agtaaatcca gatacttttt tcttcatcac atcagatgat aattcgaatt
    35641 tctcatcagt gccaaaaatg tcaggttttg cttggtaagc ttgattgatc agattggcaa
    35701 gataattagc cgaacgtatc cttgctttgc tgtccattcc agtattgtca tcgtgccctg
    35761 cccagctact gatggtaata gacggagtag agacaattag ccatgaatct cggtattcat
    35821 tggtagaacc agtttttccg acccaatctg cttttcctag atttccgttt aagcttgaga
    35881 tggcatcttt gaatggtgta gtgatttttg cattgatcac actacgcatc atgtcattca
    35941 tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt gctttcgtgt ttataaatta
    36001 catttcctgc gttatctgtg atggaatcga tgatatagcc ttcttcatag acgccgccgt
    36061 ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt gacatctact gtacctaaag
    36121 gagcagattc aacgccccaa ttgtcatttg ccgggtagtt catctttgcc aagtagtgct
    36181 cgtaagcata ttgtttcgat ccaccggaat tcaacacatc ttgatataag tgataagccg
    36241 ggatattgtt ggaccattct agtgactcac ggacggtttg gaaggtgttc gatccttcgt
    36301 tagtagcatt gacgattttc tcgccagcat tttctccttc ttgccaagtt gttgcataat
    36361 ctgatacacg ggattctgat ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa
    36421 ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct
    36481 gactgtaatt ccgtccgcca acgaatccgt agatgcggcc agttctgtta tccatcaata
    36541 catttccggt ttctacttga ctgccagcac catcatctaa catgtatcca taattttcaa
    36601 cgccattttg caatgcagca tagacatctt tatcgattgt cgaatggatt gtatatcctt
    36661 tattttggat cgtttgctgt gccttttgat aataagcgtc actgaccgat gaatcagaca
    36721 tatcagcgtt gtctttttca gccaattgtt gtgcaatgat tcttgttgct tcattcatca
    36781 cagtataata caagaattct ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt
    36841 ttgtaaggtc ataggctttt gcttcttcgt attgttcttt cgtaatttgg ccttcacgat
    36901 acatactgaa taaaacaaaa tcttttctgg caaggccggc agaaagatct tcttttagcg
    36961 cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg gggtaatcca gcgatataag
    37021 cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa gattcctttt gcagcttcct
    37081 ggactccagc aatgttttgt cccttattat tacgaccaaa tggtgagaca ttcaaatacg
    37141 tagcaatgat ctcatctttt gaaaagtatt tttctacttg tgcagaaaga atgatctcgt
    37201 ttgctttacg tttgaatgtt gtttcatctg taaggatctg ctgtttgact aattgctgtg
    37261 tcaaagttga tccaccagaa gagccaatac cagtagcttc agatactaat gctcgtaaaa
    37321 cagctttagg aacgtaacct tggtgtttat cgaagtattc atcctctgtg ctgataattg
    37381 cagttttcaa taaaggtgaa atcttatcag atgaaatagt cgtgcgcatc agatctgtct
    37441 ggatttttga tatctctgta ttatctgcat aaacaatctt tgacgtttct gtaatgtttc
    37501 ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt gtcttcgacg agataggcaa
    37561 aatagccagc gcctattcca atacctagag ctgcgaacat caaaagaaga acagtaataa
    37621 aaacagtcaa tgattgaaaa actctaaaaa taatcttagg aacaaaccat ttttcttttt
    37681 ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg
    37741 gttttatttc gctaattata gcaaataata gaacaaaaaa acagacaagg ctgtttttgg
    37801 caggaatttc ttttttaaaa gagttaaaac gtgagaaaat gatctttatt ataaaagagt
    37861 tcttaagctt ttctttagag aaaatttcag ttactatgga gaacagggca aagaaaaatc
    37921 aatcattttc tttgtatatc atcttaccaa tatataggaa ttgttagtaa caattgcctt
    37981 tcaaggagga atccatccat gttattaggc tcacacgtca gcatgagcgg aaagaaaatg
    38041 ctgttaggag cagcagcaga agcagccagt tatggatctt cgacttttat gatctataca
    38101 ggcgcccctc aaaacactag aagaaaagcc atcgaagaga tgaatattga tgccggaaaa
    38161 gcgtacatgg cagaaaacgg tctaacaaat atcgtcgttc atgcccctta tatcattaat
    38221 ttggggaata cgatcaaacc agaaatgttt ccttttgccg tccaatttct gagagaagag
    38281 attgttcgtg cggaagcttt aggcgctact caaattacac ttcacccagg tgctcatgta
    38341 ggggcaggaa cagaagccgg aattgctaga attattgaag gcttgaatga agttttgaca
    38401 aaagaacaga cggcacaaat cgctttggag acaatggcag gtaaaggtac ggaaatcggt
    38461 cgaacatttg aggaattagc gcaaatcatc gatggagtaa aattaaatga aaaattatcc
    38521 gttacttttg acacctgtca tacgaatgat gccggttata atattaaaga agacttttca
    38581 ggtgtaatgg aagagtttga taagatcatc gggttagatc ggcttaaagt actccatatc
    38641 aatgattcta aaaatccgca aggttcgcat aaagaccggc acgccaatat tggttttgga
    38701 acaatcggat tcgacacgct gaataagatc gtccatgatc caatgtttga caatatttcg
    38761 aaaatcttgg aaactcccta tgtcggcaaa gataaaaaga aagcttacgc accatatgga
    38821 aaagaaatcg ccatgttcaa agcacaaata ttcgatccag aagtgtttcc tgagttatta
    38881 gcagaagaag tgatactcta agattttcta tctgacagca gcaaaaaagg accaggcaca
    38941 ttgccttgtc cttttttgtt taagaaacaa aggagtactt tttgcagtct ttttcatcat
    39001 gcatcttctc ccccctcttt ttaaatttag acaaacaagg tatactgtta gaaaagtaaa
    39061 aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg gaaggacgaa aagatgctga
    39121 acactttaaa acccttgatc aaccaactaa aaaagaaaca tacatacttt attcttggca
    39181 tagccctatt atttacaatt attcgtttgt atttatatag taaagcaatc tatggaacag
    39241 acatgtcggc aacctatgat gaccagctgc tgattcgcta tgctcaaaat attttggacg
    39301 gtaaatggct aggggagtat acgactacaa cactttccaa aggaatctcc tacagcttat
    39361 ttttagtact tgcacataaa ctgcatcttt cttatgctgt tttgttcggc atgttcagta
    39421 tttttgccag tatattggtt gcactcgcgt ttaaaccgat cgtgaagaac aagtatcttc
    39481 ttttaagtat ctatttttat tttttgtttt ctccgattag ttttacacat gaatattcca
    39541 ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga gatcattatc gcttgtttac
    39601 tagctattta ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg tttttagggc
    39661 tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga ttctatctgg cttcttcctt
    39721 ttactgtagt agctttgggt atcagtttcg tccagatctt gctagggcaa gacttttctt
    39781 tcaaagaagt aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg gaatggctga
    39841 aactttcatt gtttttactt cctatagttg gcttttccat cgtctatcag tggatcgaat
    39901 ataaaaatga gacaacatac ggtattgcaa ccatcaatga ccgttccagt ggagaatttg
    39961 gacgtttgat gaaacaactg atccgaatag atgatgaaac gaacttgaat aaagaaaaca
    40021 gtgagatatg ggtctcacat gctgcactaa ataaagcatt agccgcatct cctacattcc
    40081 atactgtttc tacccgtatt gacgaattat atacgactca cgcgtggact caaggtggaa
    40141 aagtaaaaga attacccgga gatatcatct tttgggcatt gcgagatgtg gtaagcgaat
    40201 ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag
    40261 aattagaaga agcctatcac aagggaacca tcaaaaagaa aaaagagttc tatctgatgg
    40321 gatcaggaga tggaaagacg atgagtgatc tgccaatcgt attcgatttc cttcaatcag
    40381 gatacgctta tgcatggcga tataaagatt tccatcaagg aggaaattat agtttcggaa
    40441 cgcaagaaca agtcaaacag gctcaagaat tattaaacct tccgctaatg gagaattgga
    40501 tcgatagtaa tgaaccgaga acacctaatt tccaattaac aaaatcagca aaagtcgcga
    40561 atgtcatcat aaaaatttac caagttctgg ctattcccgt gctgattgct tcaatagcag
    40621 ggatattact tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt
    40681 cctctttgat tgtggtcacc ggattaatac tgacacaatt ggtttttctt ttcggtgtct
    40741 cgtggttttg ttcttatgcg ccagaaaaaa aggattattt tttaagtgtc tacactggcg
    40801 caggcgtgcc attaacgcaa ttcatcgtag taacaagcat gctagttttc agtaaattaa
    40861 atctgaaaaa ggctttatct tctaaataaa aagacccttg gtagctagat ttttaaccta
    40921 actatcaagg gtctttgtgt ggaaaaatca gtttctattc gatttcttct accgtttcct
    40981 gcaaacgctg ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca
    41041 ggagaaggac aagaacaatg attacataag cgatgtgatc agccaatgct agttgagcag
    41101 aagtggcttg ttcaccaaag aaactttcga tctgtcctgg taatcctcgt aagttcaagt
    41161 aagttagact aatcacagag aaccagccaa gaagttttac gatgaatgaa tttttaaatc
    41221 ggctacccat ctccgcttgg ctgttggtca tcatcagtaa agggatcatc gaaaacggca
    41281 acgcaaaagc caagaacacc tgggattcat tcatcaagtt attcaaagcg gtatgttctt
    41341 cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca
    41401 atcgtgtgac cagtcttctt agccaaatcg ggatacgcat atggatgaag ccttccatga
    41461 tgacttgacc ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta
    41521 cagcaaataa agtggataag ataccagatt ttgctacagc aatcaagata ccattgctca
    41581 tagctgatgg atcagataag gcttcataca gaccaaaaaa ggaaggatct tggacagaac
    41641 cggttttgaa gacagcgaca cccatgatca atagcagtgc attgactaga aaagccatcg
    41701 acaattggat attagagtcc caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt
    41761 cttggtgatc aatcttccga gtttgagaaa ctgctgaatg aagatataag ttatgcggca
    41821 tgactgttgc accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg
    41881 gtgagtctga gaatgtatga ctgttaggga taaagccagc aaataaactg tgccaatctg
    41941 ggttagacaa gccaacttga tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa
    42001 ccagtgcttc gatcttacga aacccgactt ttgtcagtag taagagaacg aaaacatcta
    42061 atacagtaat aaagacggca atcaccaatg gaatatcaaa aagtaagtag agggcgattg
    42121 ctgcaccaat gacttctgcg atgtctgttg ccataatggc taattcagtc aagatccata
    42181 aaatgacacc caatgtctta cttgtgcgtg ctcggattgc ttgggccaaa tccatttggg
    42241 tcacgattcc taattttgct gccatatact gtaaaagcat agcaatcaga ctagacatca
    42301 aaataactga catcaataaa tattggaaat tttgtccacc agtgatcgaa gtggaccagt
    42361 tacctggatc catataacca actgctacta atgcacctgg tccagagtaa gcaagaagtg
    42421 ttttaaaaaa acttttcccc tttggtacat caactgttcc atttacttca gctaaagata
    42481 aaccatttgt atgttcaata agtcgatgct tatgaaattg tttgttggat tgattattca
    42541 agaaaaaacc acctttccta tttatctacg attacattct acacctcaaa aagaaaaaat
    42601 taaagaaaaa tattcggtat gcctaattta ttttcgtggc gcgtcttgtt agatgacgaa
    42661 agaaattttg ttctcctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc
    42721 tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg aaatagaaag
    42781 gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt gctttgctaa
    42841 tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa
    42901 atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatggga caaaaaccat
    42961 ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc
    43021 tgacccaacg actttttacg ggctgatttt ttcagtccgt tttttatttt gtaatttgtt
    43081 agactattca gattattcag caaaaagaca agcacagaca atcctaggct cttgataaag
    43141 acaaaggagg agtaaaatgt cgatttttag aaagttaggc tggttttttc gtcaggaaaa
    43201 gaaacattat ttaattgggg ttttctcgct attcgcagtg gccttggtac agttagttcc
    43261 tccaaaagtg attgggatca tcattgatga gattgccgat aaaaatttat cagtaaagat
    43321 cattttcggg tggatcttcc tattgattat cgctgctttc ttacaatatc tttttcgtta
    43381 tatctggcgg acgaatattt ggggaagtgc agctcggttg gagaaagaac ttcggcagca
    43441 gttattcaaa cattttacaa aaatggacaa tctcttttac caaaaatatc ggactgggga
    43501 tttgatggct catgcaacga atgatttgaa tgcgatccag aatgtagcag gagctggtat
    43561 cttgaccttt gcagattcag tgattacggg cggaacaacg atcattgtga tgattttgtt
    43621 tattgattgg cgcttaacac tgattgcgct gattcctctg cctttgctgg ccgtcacttc
    43681 acgagtgctg gggtcgaaac tccatgatgc ttttcgtgat tcgcaagaag ctttttctag
    43741 tatcaatgat aagacacaag aaagtatcac tgggatcaaa gtcattaaaa catttggaca
    43801 agaaaaagaa gatctagcgg attttgaaga aaagattgaa gatgcaatta ttaaaaacaa
    43861 acgagtcaat tttctagact ccttatttga tccattcatt accttgatta tcggtctgtc
    43921 ttatgttgca acaattattt taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg
    43981 gcagatggtc tcatttatta gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg
    44041 tcgattgttc aacgtattgg aaagaggaaa tgcttcttat gatcgcgtca atgaactttt
    44101 gcatgaaaaa acgcatatcg tggaacaact gggtgcagtg cagaccccgg taaaaggcga
    44161 gctgaatgta gctatttcct cttttaatta tccgaaagaa gaatcaccag cattagaaca
    44221 aatcaagttt gcaatccaag aaggagaaac attaggtatt gttgggaaaa ctggtgcggg
    44281 aaaaacaacg atcatcaaat tactgatgcg tgaatacgat gattatcaag ggaaaatcca
    44341 atttggcgga cgttcgatca aagaatattc attgaatgct ttattaggat caatcggcta
    44401 tgtgcctcag gatcacttct tgttttcgat gaccattcga gataatatcc gtttttctaa
    44461 tccaaaactt tcagaagaag aagtagagca tgcggcttct ttagcattta tcaacaatga
    44521 aatcaaggaa ttaccaaaag gttatgatac cttagttgga gaacgtggag tctctttatc
    44581 tggcggacag aaacaaagga tttcgattgc tcgagcattg atcaaaaatc ctgaattatt
    44641 aatattagat gatgcacttt ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa
    44701 tttaaaagaa ataagaaaag agaaaacaac tatcattgca gctcaccgtt taagtagcgt
    44761 gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa atcattgaac gaggaaccca
    44821 tcatgagctg cttgcactag gcggctggta tgaacaaatg tgggagaaac agcaattaga
    44881 agcgaagata gaaggaggcg aggcctgatg gatgaaaatt atcaatcgga atggacaaaa
    44941 tcgattccac ttaaagaaca gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct
    45001 tttagaacaa cgttcatcgt tgctatctta tttgcgtttg ctctgtctgt gatcaatgtc
    45061 ctattgccaa ggatcatcca aacttttatg gatgatcatc tggctaaaca gtcggctacg
    45121 acacaagtta ttttgttttt tgctggtatg tacttatttg gagtcctagt aaaaagtatc
    45181 atttggtttt tccaatggta tttgtattcc atggcttcat tgaagaccta tcagtatatt
    45241 agggtaaaat tatttgaaaa gctgcataca ctaggtatgc gttattttga tcagacaccg
    45301 gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa cgctatttga attttggtac
    45361 gtctttttga tggtattgac tgggatcttt gcagtcgttt cttcattcat tgccatgttt
    45421 caaatcaatg gagaaattgc cttgtggact ttggtgttct tgccgatttt aggaattgtc
    45481 atctggtatt accagaaatt tagttctcgt atttaccgaa atatgcggga aaaattaagt
    45541 cagctgaata caaaattgaa tgaatcgatt tccggcatgc gtattatcca gcagtttcgc
    45601 caagaagctc gtttagctaa agaatttgaa caagtgaaca atgagtattt agacacgcgc
    45661 tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga tcatcaattt tctatatgca
    45721 ttggcgattg cgttggcgtt gactttattt gggatcgatg cgttgcattc tcccgtagaa
    45781 gttggactga tctatgcatt tacgacttat gtccagggat tttttaatcc aatgtctcaa
    45841 atgatggact ttttgagcat cttcacagat gggatggttg ctggtagtcg tatcttgaag
    45901 attatggata cggaagaata tgcaccacaa caaaaccctg gagcagatca aatgattact
    45961 tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg atggagagca caatgtatta
    46021 aacaatatct cttttgttgc taatccggga gaaaccgttg cattagtggg acacaccgga
    46081 agtgggaaaa gctcgatcat caatgtgttg atgcggtttt acgaatttta tgaaggtcag
    46141 atattgattg atgatcatga cattcgagaa tatccaatgg aagagttacg taagaaaatg
    46201 gggctggtct tgcaagatgc tttcttgttt tatggggata tcgcaggaaa tattcggatg
    46261 atgaatccag ctatcacaga cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac
    46321 aaattcatcg atgaattacc aaaaggatat cactcaaaag tgatcgaaca tggtgccagc
    46381 tattctagtg gacaacgaca gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa
    46441 attctagtat tggatgaagc aacagcaaat atcgatactg aaacagaagg attgatccaa
    46501 gaaggattgg cgaaaatgcg acaaggacgg acaacgattg cgattgccca tcggctgtca
    46561 acgattcgag atgcagaatt gattttagtt ttagacaaag gtcatatagt agaacgcggg
    46621 aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag atatgtacaa actgcaaagt
    46681 agtggtatat aaaagatgtt gtgacagtag cattttgacc taagcactag agtgaaaata
    46741 aaacatagct tcccatattt ttcatctgtg agagttagtg tcgtagttac cagatttaaa
    46801 aaagacgacg tcagctagtc agaagtgcct aatagcccct aagaaagcgt tgacaaaatc
    46861 gtttgctttc gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag
    46921 ctaaacctga tgagtctccc atggtttcat ggacggcttt ttgggttttt ctttttgttt
    46981 tggatacagc cctatatatc atggaaagga gcaaaacatg ctgattgaga aagtactgaa
    47041 taacaacgtc gtaataacaa gggatgaaag agatcgggaa atgattgtga tgggaaaagg
    47101 attggcattc aaaaagaagg tgggtgaccc gattcttgac tcatgcatcg acaaaaaatt
    47161 tcagttgaca aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct
    47221 tgagtactta gaaatttctt gtgaaatcat tgaatataca aaagaggctc tgcaagtcca
    47281 tttaaatgat tctgtgtata tttcattgac ggatcacttg tatacagcta tcgaacgtca
    47341 aaaaatgggg atctttgtgc caaatgtatt gctgtatgat atcaaacaac tgtttcctaa
    47401 agagtatgcc attgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt
    47461 atcagaagac gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag
    47521 ccagcaagac aatacctacc tgtttaccac aacaatacag gatattttaa atatcgtccg
    47581 ctattactat aagattacat ttgatgaaga ttcagtccac ttttttcgat tcgtcacaca
    47641 cctgcgtttt tttctgacac gtgtcctaga tggagcgaaa tcagaagatg gagaagtttc
    47701 tgaggaattg ttccaagtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa
    47761 aatagcagac tttttaaccg gggctttcga ctaccagatg tccacggatg aaaaaatgta
    47821 tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaagga
    47881 ggacaaatta acaagaacgt atgaaataag ggtagtgaca ttcagttggc taaaccttcc
    47941 actaattaat tggagggaat agaaatggga aaataccagg aattagcaga aaacatcgta
    48001 aagaatatcg gaggaaaaga aaatatccgt agcttgacac actgtgtgac gcgacttcgt
    48061 ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt tgaaaaagat ggaagggata
    48121 gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa tcgggaatca tgtgccagat
    48181 gtctatgcag aagtactgac agtagccggg atttccgatg aagcagaaga agaggtttct
    48241 tcaagtagta atctgttcaa tcgattaatc gacatattaa gtggctgttt ccagcctttt
    48301 ttaggtgctt tggctgcagc gggaatggtc aaaggattaa atgctttgtt cttgtttctc
    48361 aaattttata ctgaaggatc gggtacgtac atcatgctga atgggatagg ggatgcaatt
    48421 ttttatttca tgccagtgat cttagggtac acagcagcga aaaaatttca tctgaatccg
    48481 atggtggggc tcgtgattgg tgcagcactt tgttatccga ctgtacaagg agcagcacta
    48541 caaagtggtt tccctgatgg cagtcctgcg ccttatagct tgctgggtct tccggcttat
    48601 gaaacatttt tgggtatccc atgggtgggc agcagttata ctagtagcgt cgtacctgtt
    48661 atcttcatta tcgcatttgc cggacaagtc caaaaaattg ccaagaaatg gattcctgca
    48721 gtcgtccaga cctttttggt ttctttttct gtgttattaa tcgcacttcc tgctggcttt
    48781 ttgattatcg gtccgattgt cagtatactg acagatctat taagcacggg attccaagca
    48841 ttgatggact tttctccagc tgtgtatggt gcagttctag ggtttctttg gcaagtactg
    48901 gtgattttcg gacttcattg gagtattatc cctcttgcga ttatgcagct agcgcaaaat
    48961 gggtggagtc agatgttagt aggcgtttat ggcgcgagtt ttgcacagac agcagtggta
    49021 ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg cgctttgcat gccagctgtg
    49081 atttcaggaa tcttcggcgt aacagaaccg gctatttatg gaatcacctt accgaagaag
    49141 aagccattta tttactcgat gattggtggt gctgtttcag gaatctattt gatggcatct
    49201 ggaacaatca gctacataat gggcggatta gggatttttg gggtggtaaa ctacatcacg
    49261 gaaacagacg ccagtggtat gtttcaagcg tttatcggca ttgctattgc ttcggtaatc
    49321 gggttttcgc tgactttttt cttttggaag gatgaagcag gtgaaacaga tgctatacag
    49381 aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc tccactaaat
    49441 gcagcgaagg atgcagcttt tgcacaagga acactaggtc gaggaatctt aatctaccct
    49501 gaaaaaggag aagtacgtgc cccttttgac ggaacagtta tgaccctgtt cccgacgaaa
    49561 catgcaatcg gaatggtttc tgaaacaggg ctggaattgc tgattcatgt cggattggat
    49621 actgtgcagt tggaagggaa atattttgaa agcttagtgc agcaaggaga aagagtgaga
    49681 caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg aaaaggaagg atacagttta
    49741 gaaacacctg tgatcgtcac caattacacg gattatttgg atattttgga aaaccaaggg
    49801 gagacggtcg ggccagacga tgcacttatc accgcactga cctgaacggt taggtgaaga
    49861 atggactagg cttaactatt ctttttattc cacttcttta ttttcaaaat tgaaaggctc
    49921 tttcatgatg gctgagctga tagggcgttt atcgaatttt tgaccgacag aaattagatg
    49981 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    50041 acaaattgat gtattgatcg taaaattacc aatttttgaa tacagtcgct cgcgtgtcgt
    50101 aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa tcaccgatac tggtcaaagc
    50161 taaaattgga acatcatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    50221 acccgtataa gaaatcagaa tcccgcacat atcgctcgta caattgtacg cttcatagaa
    50281 tgattcacca aaagtggtga caattgtgac atccattttg atgcgcttca ttttcagcat
    50341 gaaatcttga gaaatcaaag tattcgagtt actggcgaag atcacgattt tttttgcctg
    50401 gagcagtaaa tatttagctt tttgcagttg gtcatgatga atgagagaaa gtgtatcatg
    50461 aatcgtcatc tgttctaaag aagccatttt atttgcgatt gtcataatac cgtctgtggt
    50521 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    50581 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    50641 gagtgtagac gggtggacat aacatcgctc agctatcgct tggatggtca tttcgccaag
    50701 tgctggagaa tcgcttaaga gaaactggat cacttcattt tctgaaggag aaaaggagat
    50761 ttctttgatt ttttcacgta ataacatgta gataccttct tcttattttt ttacgtaaat
    50821 tataactttt ttgaaaatgt tttgtaaacg gatttatact atataactta aaataggctt
    50881 tttttgacaa gatatttaca aaaatccact agaaaacagt aacttttgta aatgttactg
    50941 gaaagaaacg gatttctatt gtttgttttg tttacaaaaa agataatgaa agtgtaaaaa
    51001 agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc tacgattggt ggaggcagca
    51061 gttacacccc tgagttgatg gaaggattta tcaaaagata tgaggaatta ccgatacgag
    51121 aaatctggct cgtagatgta gaagaaggaa aagaaaagct tgagatcgtt agtcagatgt
    51181 ctcagcgtat gtgggatgct tctccatatg aagtcaagat ctatgcaact ctggatcggg
    51241 aagaagcatt gaaagatgcg gattttgtta cgactcagtt tcgtgtcggt caattagatg
    51301 cacgaatcaa agacgagcgg atccctagtt attatgggat gattgggcaa gaaacaaatg
    51361 gtgctggcgg gatattcaaa gcttttcgta ccgttccagt cgttttggca atcgttgagg
    51421 acatgaagcg tttatgtcca aatgcttggc tgatcaattt cgcgaaccca agtgggatgg
    51481 tgacagaagc agttatcaga tatggcaaat gggataaagt gattggatta tgcaatgtgc
    51541 cagtgatggc gatgatgaca gaaccagcaa tgcttggcaa gaaaaaagaa gagctgatct
    51601 acaagtttgc cggtctaaat catttccatt ggcacaaagt agcagataat aaaggaaatg
    51661 accgcacggc agaattgatt gatcgtttat acgatgaaga taatggatta ccgaaaaata
    51721 tctttgatat cccgtttttc aaagaacaat taaaacaaat gaacatgatc ccttgtggct
    51781 accaccgcta ttactatcgt gaagaagaaa tgctggaaca tgctttagaa gaatatcaaa
    51841 cgattggtac aagagcgcag caagtgaaag aaacagaagc agagctattt gaattataca
    51901 aagatccaga attagaccat aaacctgaac aattgcaaaa acgaggtggc gcttattatt
    51961 cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa taaagaaaca cagatcgttg
    52021 tatctacaaa gaatgatgga gctgttcctg acctgtcggc agattgtgtt gtagaagtca
    52081 ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt tggttctctt cctgctgctg
    52141 aaagaggctg gttgcaagta atgaagaata tggaattgct gacgatcgaa gcagctgtga
    52201 ctggtgatta tgggacagct ttacaggcgt ttacgatcaa tccacttata cgatcaggaa
    52261 agacagcaaa acggatcatg gatgagttgt ttattgcaca ccagcagtat ttaccgcaat
    52321 tcaaggaaac aatcagccga ttaaaagaag aaggaatcga agttcaagat gaactggcaa
    52381 gagaattatc aggtaataca catataatgg aggaagtata atggggttca gaaaagattt
    52441 tttatggggc ggtgctacag cagccaatca atgtgagggc gcttatgatg aaggtggacg
    52501 cggactagca aacgtagatg ttgtgccaat cggaaaagac cgttttcctg ttatcaccgg
    52561 aaagaaaaaa atgtttgaat ttgatgagga tcatttttat ccagcaaaaa ttgcaatcga
    52621 tatgtatcat cgttataaag aagatattgc tttgtttgca gaaatggggt tcaagactta
    52681 tcgaatgtct atcgcctgga gtcgtatttt tccaatgggg gatgagaaag agccgaatga
    52741 agctggcttg aaattttatg aggatgtctt taaagaatgc caaaagcacg gaattgagcc
    52801 acttgtgacg attacgcatt ttgattgtcc aatgcatttg atcaaagaat atggtgcgtg
    52861 gcgttccaga gaactcgttg gtttttatga aaatctgtgt cgagtcatct tcaatcgcta
    52921 taaaggactt gtcaaatatt ggctgacatt caatgagatc aatatgatcc ttcatgcacc
    52981 atttatgggc gcagggctgt attttgaaga aggagaaaat gaagaacaaa tcaaatatca
    53041 agcggcacat catgagttgg ttgcaagtgc catcgcaacg aagattgctc atgaagtaga
    53101 tccggagaac aaagtaggct gtatgctggc agcaggagca tattatccat acacttgcaa
    53161 tcctgaagat gtttgggctt ctagaaaatc agatcgagaa aattatttct tcattgatgt
    53221 ccagtcacga ggagaatatc ccgcttatgc attgaaagaa atggagcgaa atggcattca
    53281 gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa catactgttg attttatctc
    53341 tttttcttat tattcttcac gagtttcgac taccgacaac gaactcttgg aacagactgc
    53401 aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa gcaagtgaat gggggtggca
    53461 aattgatcca ttaggcttga gaataacgat gaatgatatt tatgatcgtt atcaaaaacc
    53521 attatttatt gtagaaaacg gcttaggtgc agtcgatacg ccagatgaaa atggttatgt
    53581 ggaagatgat taccgaatcg attacctggc agcgcatatc caggcgatga aggatgcagt
    53641 agaactagac ggagtcgatc tattaggcta tacgacttgg ggatgtatcg atttagtatc
    53701 agctggtacc ggtgagatga agaaaaggta cggattcatt tatgtcgatc gtgataacca
    53761 aggaaatggc acattagccc gttcaaagaa aaaatcattc aactggtata aaaaagtaat
    53821 tgattcaaat ggagaaaatc tttcgaacaa ttgatcaatg atttataaaa gtctattgtt
    53881 tttcagcgga tttttcgagt ataataaagc tattcgagaa acgcaagcag aacaatagac
    53941 tttttttcgc attggctgta aagtatgctt acttgattaa gtaaaggaga atctacatat
    54001 gtatgatgta atcgttattg gcgcaggtcc tgctgggatg actgctgctt tgtatgcttc
    54061 acgttcaaac ttatccgtat tgatgatcga acaaggtgcc cctggcggcc aaatgaacaa
    54121 tacggcagaa atcgaaaatt atcctggatt cgattcgatc atgggccccg atctagctta
    54181 taagatgtat gaaagtgtct caagatttgg gacagaaaat gcttatggaa tcgttcaaga
    54241 tatcaaggat cacggcagcc ataaagaagt catctgtgaa gataaaagtt acgaaggaaa
    54301 aacagtcatt atcgcaacag gctgtatcca ccgtaaatta ggcgtacctg gcgaggaaga
    54361 atttgctgga cgaggcgttt cttactgtgc agtctgtgat ggtgcgtttt tccgtaacaa
    54421 acgtctagta gtcatcgggg gtggggactc agcagtagaa gaagccatct atttgacaca
    54481 atttgcttct gaagtagtga tcgtccaccg tagagatgaa ttacgtgcac aaaagatcat
    54541 ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg tgggatacag tcgttgacga
    54601 aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca cgtaatgtca aaaccggcaa
    54661 tgaatttgat ttagcagcag acggagtatt catctatgtt ggactggatc cattaactgc
    54721 accttttaga gaagcaggtt tgacgaacga agctggctgg attccaacag acaacgaaat
    54781 gaaaacgaat atgccaggcg tttttgccat cggagatgtt cgtgaaaaag atttgcgaca
    54841 aatcactaca gctgttggtg acggcggtat tgctggacaa caagtgtata aatacttgga
    54901 gtctttagaa gaaacaacag cataagttta aaaagagtga aagaacggtt tcaattgttc
    54961 ttttactctt ttttttcgcg tgaaatccac tgaatccgat ttatgtgaat acttgcaatc
    55021 atgcttgata aaagaatcct ttcttgattt aagcgttttt tggtcctcat actttcatat
    55081 ctaataagtg aatgctatac ttattaagaa ccatatttca gaaagaggtg ttattttcat
    55141 gtcttgggaa caagtgtatg aacaatgggt acatgaagaa aatttagaag aaaacttaaa
    55201 aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt gaagatgctt tttatgcacc
    55261 gttggaattc ggtactgcag gcatgcgggg aatcttagga cctgggatca atcgaatgaa
    55321 catctatacg gtaagacaag caacagaagg actagcacgt ttcatggata cacaaggtga
    55381 agaaacaaaa cgccgaggtg tcgctatcgc ttatgactca cgccatcagt caccagaatt
    55441 tgcaatggaa gcagccaaaa cactggcaca tcacaatatc ccgtcttatg tattcgaaag
    55501 tctgcgtcca acaccagagc tttcttttgc agttcgtcat ttgaagacgt ttgcgggaat
    55561 catggtaacc gcatctcaca acccagcggc ttacaatgga tataaagtct acggagaaga
    55621 tggcggacaa atgcctcctg cagacgcaga tgcattgacg aaatatgtcc gtgaagtcag
    55681 caatccgcta aaagtcgaag tattatctga cgaagaagct aaacatagcg ggttgatcac
    55741 gatcatcgga gaagaagtgg acgctgctta tctagaagaa atcaaagcag tgacgattga
    55801 tcgtgaattg gttgaaacaa tggggaaaga tttgaaactt gtgtacacgc cacttcatgg
    55861 aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct ggattcgaac aattcgtact
    55921 agttcccgaa caagctgtgg cagatccaga tttcactacg gtcaaatcgc caaatccaga
    55981 agaacattca gcttttgaat atgctatacg acttggagaa aaagagggag ctgacttgct
    56041 gattgcaaca gatcctgatg cagaccgttt aggcgcagcg gttcgtttgc cagatggttc
    56101 ttatgaagta ttgactggga accaaatcgg agcattgttt gctcaatata ttttggaagc
    56161 acatcaatca agaggcactt taccagaaaa cgcagcggta ttgaaatcaa tcgtttccag
    56221 cgaattgcct acagcaattg cggaacacta cggtgctact atgttcaatg tgttgacagg
    56281 gtttaaattc attgctgaaa aaatccaaca atttgaagaa gaccattcac atacttttat
    56341 gtttggattc gaagaaagtt atggttattt agttaaacct tttgttcgcg ataaagacgc
    56401 gatccaagca ttgcttttat tagcagaagt agcagcttac tataaaaaac aaggcaaaac
    56461 gttgtatgac ggcttgcaaa cgatttttga aacctatggc tatttcgcag aaaaaacgat
    56521 ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc tctgctttga tggaaaaatt
    56581 ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt attcaaacag aagactttaa
    56641 agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa ctaacaacac cgccatccga
    56701 cgtattgaag tatacattgg aagaaaatgg ctggatcgct atccgccctt caggaactga
    56761 gccgaaaatc aagttttata ttggtgtaaa aggaagctct acagaagacg cagccgaaaa
    56821 agtagcctct tatgaagcga ttatcaaaaa gatgacagaa gaataaaaaa cttgagactg
    56881 atggaaaaag tggtaaactt tagaagtcag tcttttttca agttggaggt gaatggattg
    56941 ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat tattcaaaat gttgagtgac
    57001 cccactcgtc ttaaaattct gctatatctg aaagacgggg aacaaaatgt gacagcaatc
    57061 acccaagcag tggaaatgga gcagtctgct gtatcgcatc aactacgttt attaagagaa
    57121 aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt tgtatagtct tgatgattct
    57181 catgtactgg atattttgaa ccagacactc aaacatgtgg aacatcaata acgataggtg
    57241 caaatagata aacactggag tgtgacaacg aaatttcttc gttgtcacac tccagtgttt
    57301 tttaagaaaa agacaaaaat gattaatcgc ttttttctgt tgtttcagtt ccataataac
    57361 tatgatcttc taggtctaaa gcattcaaaa taagcgaagc cgtttcttca atagataaag
    57421 atgccacatt gatcactaca catcctagtt tttcataaag atcattcgca aaatcaagtt
    57481 cagctctaat tttttcgata tcggaataag cagtatcagg gtttaatccg taagagcgca
    57541 tccgttcttt acggatatta tttaaaatat ctgggttatt cgttagtccc acgatttttt
    57601 taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa ggtaaattgg
    57661 caacttttaa atttttgtta gctaaaaaca agctcaaagg cgttttggaa gttcgggata
    57721 cgcctagcaa aacgacatct gcttctaaaa aaccacgagg atctttccca tcatcgtatt
    57781 ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt ctcattcaat aaatggaggg
    57841 caccaggttg ccgaattggc ttgataccag ttaaacgttc aatttctgat atcggtggcg
    57901 tcatcacatc aaaactaaat aattgatgtt cttcacaaaa agaacgtgtt atctcgatca
    57961 gttcttgatt gattaatgta tgcaggacaa gtgctttttc ttctaatgca tcttgcaatg
    58021 cctgccttaa tgttttttca tctgtcacga acgttctgcg gaataggttg aactcaacgg
    58081 taggatactg agccatagta gcttgcgcta gttttgtcgc agtttctcca gcagagtcgg
    58141 agatgacaaa aaaagtaacg atttctgtag aatcggaaga catggataaa acctcacttg
    58201 catttttttt atgtttatta taccatactc tagaatgcgt acttcgtaat cattttgatt
    58261 tggaggaata agtcatgaaa aaacagacat tgatcgaaca atcattagaa acattgaaaa
    58321 ataacggttt taaatatact aaaaaaagag aagcattgtt aacttattta gtgaagcgta
    58381 accgctacgt atctgctaaa gaagtttttg actatatgaa tcaggaattc aaaggggtaa
    58441 gttatgagac gatttaccgg aatctccatg attttgcaga gatcgattta ctggaagaaa
    58501 ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg tcaggggaaa atcggccatc
    58561 accatcattt tatctgtaca gtgtgcggaa caacgaaaga aatccatatg tgtccgatga
    58621 attatttcga agaacagcta ggtggatgca cgatcgaagg gcatagattc gagattttcg
    58681 gaagatgtga aaattgtcaa taaggattta aataaaataa catttgataa ttttaaaaaa
    58741 aagcaagatt tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg
    58801 ttttttgaga tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat
    58861 taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt
    58921 caaacgttcc gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga
    58981 aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta
    59041 gttctgtgat gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg
    59101 aaagcagcga tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca
    59161 tctatacaaa acgagcagat taaagaaggt cacgacttga cagaagaaga agaactaacg
    59221 gtgttgtctc gtgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga
    59281 cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa tagtcgaaaa atatatgccg
    59341 gaacaacttt ccgacgaaga aattcgacaa cttgttcaag aagcaatcac tcaaactggt
    59401 gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa ttatgcctaa agtcaaagga
    59461 aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac tattacaatc ttaattttat
    59521 catttaaact gcggacagtg attatccgca gttttttgtg ttcgtaaaaa aagaactaaa
    59581 aaacagctag aagaaagaat cttattcttt cttctagctg tttttttcct gtttaatatc
    59641 tcttatgtta aattgtgttt gatttggtta ttcttctggt aactcacttg cttcttgttt
    59701 atatcctgat acgcgatata cataataaaa gagcaagctg ccagcgtaac caagcaacgt
    59761 tgggacgacc cagccaagac ctaatgcagc aaaaggaaca taatgatgat aaaatcctag
    59821 taagcttgct acgacagttt gctgactcac taatgcaggc gaatttgcca acatatccaa
    59881 tacggctgga acggcagaaa agaacatcgt tacttgatag acaaatggac tcttttggaa
    59941 gaattttgct gttaaggaaa gtaagattaa cgcaagtgac aatggataca aaagcatcaa
    60001 tactggtaaa gaccattgga tgatattatc caatccggca ttcgcaacga caaaagagac
    60061 aaaagtcgtt aaccgcagcc atgtcatata actgacttta gggaacagtt tatggaaatc
    60121 ttgtgcaaaa gaaacaacga gtcccatagc agtagtgaag acacctaatg tgaccattac
    60181 ccctaagaag ctagcaccga agttacctaa ataaaaatta gtgatctgag aaagtgcagt
    60241 tccgccattt tctgataaag acatcttatt caaactcatc gcaccgagaa ggaccagacc
    60301 aaaataaatc atgacttcta acgcaacgct caggctacca gcttttgctg ttaattctgt
    60361 gactttttta tctttaaaac caagatcacg aactgcatgg acaaaagtga cacttaaagc
    60421 aagtaaagct actgcatcaa ccgtgttgta tccttctaaa acgccatttg tcaaagcgtt
    60481 cgtctgataa gaatctgcag cagattggtt tagattcccc atgggttgga ataaagaaac
    60541 gacaaaaatg atagctaata atgcaagaaa caaggagttt aaatactttc cgacatattt
    60601 gaccaaacgt gttggatgta cagtaaggaa ataagcaagt aaaaagaaaa gagcagagaa
    60661 taaaaacata cctaatgaat catatttttc aggcaaaaag ggtttagcag ccatttcaaa
    60721 agcagttgct gctgtccgcg gtgtaccaaa gaatggtccg atcgttaaat gaacaaggat
    60781 caagaacaag gcagcatacc attttccgac aggtttagct aagtcataca gaccatcact
    60841 ttttgtcact actacagcta agatagctaa taatgggaaa agagcgcctg agatggcaaa
    60901 tcctacacca gcgctgatcc agtttgatcc agccatttga cctaagtgta caggaaagat
    60961 cagattacct gacccgaaga acatcccgaa gataagtgaa ctgaccatca ataattgctt
    61021 ccatgaataa gtttttgttt cttccataaa tttttcctcc aataattatc attttgagta
    61081 acaaaataaa cgttgcagaa actagaaata ccactaatat cagattccat gaaaaccacc
    61141 accttaaaat ataaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg
    61201 gtaccacctt ttttcgctta aaaaagcttc ttcattcacg gacgcttttc gataaagtta
    61261 tccgctagcc tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa
    61321 atgatgttcg tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa
    61381 tcactgaata gcctactgtt ttcctcgacg ctttgctgta tatttatttg ttgacgttat
    61441 aatacacctg ttttttttga aatgcaagaa tatttcaaat attttgtttt ctttttttga
    61501 aacaagaaaa atacccagag aatacttgct aagttagacg gaatacttgt cctctatctt
    61561 atgcatagct tacatgatca gcttttttta ttcaaacgag acctttagag aatggaagcg
    61621 gctagttttt taacgtttct tggtacttat ggttaagcaa ctcttttaga accttttctt
    61681 atttgctata tagtttcttc tgagtatggt aaaataggaa agaatgaatc gaaaaaaagg
    61741 ggatgtctat ttttgacgga agagtctagt tctttagaga ttagattgac ggctgctgat
    61801 gatgtcagca tgcttttagg tgcacacgac aagcatataa aactgattga ggaaacaacg
    61861 gaaacaacca tccatacacg tggagaaatc attcagatca ttggtgagca atcccagatt
    61921 tccttggctg cctcagtcat tcggacatta caggaattga tcaaacgagg aatccacgta
    61981 agtacgcccg atgttgtgac ggcattgaaa atggggcaaa aaggtacatt agattatttt
    62041 gttgagatgt atgaggaaga gatcgtcaaa gaccgtaatg gaaaacctat acgggtaaaa
    62101 aacagcggcc aaaagaaata tgtcgaatcg gtagccaaac atgatatcgt atttggcgta
    62161 ggaccagccg gtacaggaaa aacttttctt gcagtagtat tagcaatcgc tgccttgaaa
    62221 aaaggtgaag tgcaaaaaat cattttgacg cggcctgctg tggaagcagg tgaaaatcta
    62281 ggctttcttc ccggggatct aaaagaaaaa gtggatcctt atctgcggcc tgtttatgat
    62341 gctttatacc aaatttttgg attggatcat acgaaccgat tgatggaacg aggcgtaatc
    62401 gaaatcgcac ctttagctta catgcggggg cgaacattgg atgatgcatt cgttatcttg
    62461 gacgaagcac aaaacacgac agtcgctcag atgaaaatgt tcttgacccg tctcggttat
    62521 caatcaaaaa tgattgtcaa tggggataca agtcagatag acttgcctcg agggacgaca
    62581 agtggtcttg ttcatgcaga acgaacatta aagcaaatca agaaaatcga ttttgttaat
    62641 tttgaagcaa gtgacgttgt tcggcaccct gtagttgccg aaatcatcaa agcttatgaa
    62701 aaggcagatc tccatcaaga ataagctttg cttaggagga taagcacatg ttgaataaaa
    62761 cactgcagaa actacaagaa aaacttggaa aggcagcatt gcctttcctg ctgttcttgt
    62821 tttttatcat tatctgcggc gttacattta gtagtgtcaa acaaaaggcc aatgatttca
    62881 gagaaggcca agtagcagaa gaaagtatcc gtgcgaacaa aacgatcgag aacacggaag
    62941 aaaccgaaca aaaaagaaaa ctggcagcag aagcagtcac tccagaatac acttaccaaa
    63001 aagatcttgc agatgaccag aacaatcgga tcaagcaact atttgaattg atcgataaga
    63061 cgaatgatgc catcaacaaa tcttatgacg aaaagattga taaggcaaag gaagatgaaa
    63121 atatcccaaa gcctactaca gaagagcgga tcgccgcgct gaagaaaaat tttgaaaatg
    63181 tgaatgcaga aaatgttgtg ttttatcaaa aactaccgaa caatttttat tcgacgatat
    63241 ttgaaatgac tgaaactgag attcatactg tgagggatga gagtctgaag ctcattgacg
    63301 aacagatgag caaacaagtt cgtgaaagtg aattggaagc ttttaaacaa gaagcagaag
    63361 agcaaatcca atatttgaat gtcacaccag cacaacaaca aatgattcgc tacttagtgg
    63421 atcaaggtat cgtagtgaat gacgtattga atgagaaaaa gacagaagaa ttgaaacaat
    63481 ccgcacgaga agctgtacaa ccagtgatga tcttccaagg agaaatcatt gttcgtgaag
    63541 gtaatcaaat cgatgcttca gctatgaaaa aattggaatt actaggactg actaatcaaa
    63601 caacttcgat ttttccgcta attgccatgg ttttagctgt tttgcttcaa atcggcgtac
    63661 tgatttataa ttcacttcaa ttccaaaatg caggagaaag aacaaaatac gtgcttttct
    63721 atgtgacagc tatgtcagtc agcatagtgc tgatgaaatt tttccaatta ttccaaacgg
    63781 aacaagctac ttatatcccg ctgttttatc ccgctgcttt tgcaccactt gttttaagct
    63841 ttttcttgaa tcgaagagca gggatcatgg ctggtatttt ccaagcagta tccgcacttt
    63901 tcatttttta tgattcgatt ggcacgaact ttctaacgat tatcttgatg tcttatgttt
    63961 tctctgggct catggcaacg attgtcagac gcaaacggat ctcagaacaa ggattatctg
    64021 ctgccatgtg ggtcattatt tttcctgtga tgatggatgt cattttgatc gtctatcaag
    64081 gaatgagctt tggtgatgca acgacttggt taatgctcgt ctgcgggttc actggtgcac
    64141 tcttctcatt cttgctgacg atcggtctac acccatatat tgaattgatg gtcaatgatg
    64201 acagtgtaat tgtattaaat gaactcagca atccaaatca tcctttattg aaacagttgc
    64261 tggaagaagc gccaggtact tatcatcata gtatgatggt agctaatcta agtgctaacg
    64321 ctgtagcaga aatcggcgga cgttctttgc taactcgagt agcttgctat tatcatgata
    64381 tcggcaagat caaacatgct agctttttcg ttgaaaactt gccttcagga gcggaaaacc
    64441 cgcacaattt cttattgcca gaagacagta aacatattat ctttggacat gtgacagatg
    64501 gagccaaaat attggaagat tataatatgc cgcaaatggt gatcgatatt tgcaaacagc
    64561 atcatggaac cacgctaatg cgttatttct acatcaaagc gaaagaacgc aatccggaag
    64621 taacagaaga gcaattccgt tatcctggac caaaaccgca aagtcgtgaa gcagggatcg
    64681 tcaatattgc tgacagttgt gaggctgcag tacgggcaat ggatcatccg acgagcgaaa
    64741 aaatcgaaag ttttgtccat aatcttatcg aagagcgtat ttccgatggt caattagacg
    64801 atagtggact gaccttgaaa gaaatccgaa aagtggaaaa atcattaatc agcggtttaa
    64861 gtagcacctt ccattctcgg atcaaatatc ctaaaatgaa aacagaagtt gagaagataa
    64921 aggaagaaca agaagaatag aaagaagcga tgtataatgg atattacctt tatggatgaa
    64981 acaaaccaag tctcagaaga gaaaatgaaa gaaatcgatg acttgctgca atttgcagca
    65041 tcttatttaa agctgccaga ggaaacagaa atgtctgtta ctttcatgga caatgcggca
    65101 atccaagtaa tcaatcgtga ttaccgtgga aaagatgcac cgacagacgt catcagtttt
    65161 gctctggaag aagaaggcga agacgaaatc ccagtgattt ttgaagatgg agagaatcca
    65221 ttacctcgtg tactaggaga catcatgatc tctattgaac gtgctgctga acaagcagaa
    65281 gcttacggcc attcatatga tcgggaactt ggctttttag cggtacatgg attcttgcat
    65341 atcaacgggt atgaccatat gacaccagaa gatgagaaag aaatgtttgg tttacagaaa
    65401 gagatcttgg atgcctatgg actcaaaaga taaaaacgta gagaaaaaca aacatttcct
    65461 gatgtctgtg gaatttgctg tccaaggagt caaaaccgtt tttgaggaag aacgcaatat
    65521 gaggaaacat gttgcgttcg gtatccttgc tttatttatg ggcgtgattt tccagcttga
    65581 tcggatagag tggttatggc tgcttttagc tgtttttctc gtttggatag tagagatcat
    65641 caatactgtt tttgaaaatg tagtggacat gtttacggat tttcattttc atccgattgg
    65701 gaaaaaaatc aaagatatgg cagctggagc agttttagtg accgcttgtt ttgctgtcat
    65761 cattgggttg attttatttg tccctaaaat atatcatttg ttgtttaatt aaaaaggaga
    65821 acgtatatgc cagaacataa atcagggttt gtcgcaatcg tcggccgccc gaatgtagga
    65881 aaatccacat tattgaaccg aatcgtgggt caaaaaattg ccattatgag tgacaaggca
    65941 cagacaacta gaaataaaat ccaaggagta tatactacac cagaagctca gctgattttt
    66001 atcgatacac caggaatcca taaaccaaag catcgtctag gagattttat ggtagaagca
    66061 gcttacagtg cgttgaaaga agtggatgca gtcttattta tgatcagtgc tgatcaaaaa
    66121 cgtggccggg gagacgattt tattattgaa cgtctaaaaa acgtgcattc tcctgtttat
    66181 ctgattatca ataaaatcga taagatccat ccagacgagt tgctgggaat catcgaagat
    66241 tattcgacgc agatgccttt tgcacaagtg attccgatct ctgctacaga aggaaacaat
    66301 gtcgaacgct taatggatgt attagtcagt gaaatgccag agggcccaca atattttcca
    66361 gatgatcaag tgactgatca tcctgaatac ttcatcgttt ctgaattgat ccgtgagaaa
    66421 gtcttgtttt taacacgaga cgaagtgcct cattctgtcg cagtcgttgt tgattcgatg
    66481 aagcgaaacg agaatgataa aatccatatc caggccacga tcatcgtcga aagagatagc
    66541 caaaaaggaa tcattatcgg caaaggcgga aaaatgctga agcaagtagg aacgaaagca
    66601 agaaaggata tcgaaaatct tttaggagac aaagtatttc tagaactttg ggtcaaagtc
    66661 caaaaagact ggcgagacaa aaaagtctat ctgcaagact tcggctaccg aaaagacgag
    66721 tactgatatt ctggacttct tataggttag gtatagaaat aaactggata aaaatccgta
    66781 cgttgagaaa aacagcggaa gatcgaaaca taaataatct ggatcggcag aatctgcttt
    66841 tccagattta ttttttagga gggagaatat gagccagctt gcagaatcga aaggcttgat
    66901 tttatctagc cgtaattaca aagaaaaaga taaattagtc aaaatcttta cggaatcttc
    66961 tggcaaaatg atgttttata tcaaaggaat ccatcggcaa aatcagccat tagctccggc
    67021 tttgctcccc tttaccgaag cagtctatat cggtaatttt cgaagtgaag gtttgtcgtt
    67081 tttaagcagt gtcaaagacg tccatgcgtt tcggaacatc caagaagata tctttttgag
    67141 cggttatggt acgtatttgc taaatctagt agatgcagca atcgaagatc atcaatatga
    67201 cccgcattta ttccaattta cgcaacaagc tctgcagcgg atggaccaag gagatgatgc
    67261 agaaatcatc acgaatatct ttgaagtgca aatactgcaa cgcttcggaa tcgcgccaat
    67321 ttggacgcac tgtgtcgtat gcggggaaac aaaaggaaaa tttgactatt cttccaaata
    67381 tggcggtgtg atatgcgaga aacactggcc aatggacgaa caccgttatc atgcagatcc
    67441 tcgcgctgtt tatttcgttc gaatgttttc ggctatttct tatgataaga tcagtggaat
    67501 ccacttgaaa gaagaaacga agcaggcaat ccgtgacctc attgaccagt tgtatgatga
    67561 atatgtcggt attcatctaa aaagcaaaaa gttcattgat caaatgaagt cttgggcaca
    67621 cgtattaaag acgccagaga aaaatatgcc tgaaaaagag atacctgaaa aagaagaata
    67681 aatgaaagat acaacaaaaa aatcaaaact atttgacaaa tacagggact tccgtctatt
    67741 attaaccgta gattaaatag atcgttcact gagaggatct attatacaaa ccagaaaact
    67801 gcaaaatgaa tacgtacatt gaccaagatc agtaaaaaac acgtggttta gcgagttcgg
    67861 gagagtggaa gccggaccca aacgattttt gaagaggcac ttgcgagtac gataaatgaa
    67921 gctgccgaat ctttattcgg aagtagggtg gaaccgcgaa attattcgtc cctatgtctg
    67981 taatcagaca tagggacttt ttgtcgtttt ctttcctgta ttcttattga cagcaacgct
    68041 taaaaattga aaggggcaaa acagatgagt aaaaaattga ccgtccaaga tatgatttta
    68101 acattacaaa aattttggtc atcaaacggc tgcatgctga tgcaagctta tgatacagaa
    68161 aaaggagcag gtacgatgag cccatataca ttcttacggg caatcggacc ggaaccatgg
    68221 aatgctgctt atgtagagcc atcccgccga ccagctgacg ggcgttatgg agaaaaccca
    68281 aaccgtttgt atcagcatca tcaatttcaa gtcgtaatga aaccttcacc tgaaaatatc
    68341 caagagcttt atttagaaag cttgcgtctt ttaggaattg atccattaga acacgatatt
    68401 cgctttgttg aagacaactg ggagaaccca tcgatgggtt gtgccggtgt gggctgggaa
    68461 gtatggcttg atgggatgga gatcacacaa ttcacatact tccagcaagt cggaggacta
    68521 ccttgtaaac cagttacttc tgagatcact tatggactag aacgtcttgc ttcttatatc
    68581 caagaagttg aaagtgttta tgatctggaa tgggctgatg gcgtgaaata tggtgagatc
    68641 ttcaaacaac cagaatatga acattctaaa tattcttttg aagtaagcga ccaagacctg
    68701 ctactaagca attttgatcg ttttgaaaaa gaagctaaac gctgtatcga tgaaaatctt
    68761 gttcatccag cgtatgatta tattttgaaa tgtagccata cattcaattt attagatgct
    68821 cgcggtgcag tatccgtaac agagcgtgca ggttatcttt cccgtatccg caacatggct
    68881 cgtgctgtag ccaaaatctt tgtggcagaa cgcgaaaaat taggattccc tttattgaat
    68941 aaaaacacag caaatccaga aaacgtgaag gaggctgaat aagatggcaa aagacttatt
    69001 acttgaaatc gggctggaag aaatacctgc tcatgtagtc acaccaagca tgaaacaatt
    69061 agaagaaaaa acagcaaaat ttttagatga aaaccaattg acttatgata cgatcgaaac
    69121 gttttctaca ccgcgccgct tagcaatcaa agtaacaaac attccagaac gtcaagcaga
    69181 cagcgaagaa gaagtcaaag gaccagccaa aaaaattgct ttggacgcag atggaaactg
    69241 gacgaaagca gctgaaggat ttgtccgcgg acaaggtctg acgacagatg ctatcacttt
    69301 tagagaatta aaaggcgtgg aatatgtcta tgtaacgaaa tacactcacg gcaaagaagc
    69361 aaaagaagta ttgacaggtt tagcagatgt cattaaaagc ctcactttcc cagtaacgat
    69421 gcactgggca gaatacgatt ttgaatatat tcgccctatt cactggatcg tggcattgtt
    69481 agatgacgaa ataatcccgt ttgagatctt ggatgtcaaa acagacagag cttcaagagg
    69541 tcatcgtttc ttaggcgaag atgtgacatt tacgcatgca aaacaatacg aagaaaaatt
    69601 gaatgaacaa tttgttattg cttcaccgca aaaaagaaaa gaaatgatca tcacacaagc
    69661 aaacgaatta gcagaaaaaa atcactggat cattgatcta gatgaagatt tactagaaga
    69721 agtcaacaat ttagtcgaat atccaactgt cttttctggc gacttcgaag aaaaatatct
    69781 atctgttcct gaagaagtac tagtcacttc tatgaaagaa catcaacgtt atttcgaagt
    69841 acggaacgat caaggtatgt tgcttcctca ttttatttct gctcgtaatg gcgatcgggt
    69901 aaaattggaa aatgtggtac gaggcaatca aaaagtattg actgctcgtt tagaagatgc
    69961 ggaattcttt tataatgaag acaagaaagc ttcaatcgac caatacgttg aaaaattaaa
    70021 aactgttact ttccatgaaa aaatcggtag catctatgag aaaatgcaac gggtacaagt
    70081 catcgctcgc atcataggcg aacaagtagg actatctgag gaagaattga tggatctgca
    70141 acgcgctgct gaaatctata aatttgatct agtaaccaac atggtaggag aattccctga
    70201 actacaaggg atcatgggcg aaaaatatgc tctgcttaaa ggcgagaaac cagccgtagc
    70261 aacagcgatc cgtgaacatt acatgcctat atccagtgaa ggagatttac ctgagactac
    70321 agtaggttct gttttagcga ttgctgataa attagatagt atcttttctt tctttgcagt
    70381 tgggatgatc ccaagtggct caaacgatcc atatgctttg cgccgtcaag catatggtgt
    70441 cgttcgaatc atcgaatcga aaaaatgggc ctttcctttg tctgttctgc aagaaacgat
    70501 tagtgaagtt atcagtaaag atacggatcg ttttggcatt ggcttgtcag cgggacaaca
    70561 acaggtgatc gactttatca aaggccgtct gcgccaatta ttgacaacga agaatatccg
    70621 tcatgatgtc atcgaagctg ttttgaatgc agaacaaaaa gatctgacaa aagtatttgc
    70681 agcagcacaa ttgttcaagc agcatcttgc agatgaagac ttcaaaccat cgatggaagc
    70741 tttgacacgt gtcgtgaact tagcgaaaaa agcggagctt gaaaaacaat cagaagtaga
    70801 ccctgaactg ttcgaaaacg aagcggagaa agaactgcac aaagcagtag aggcaatgaa
    70861 aacaagcttt gctgaccaaa cgatcaacga aaattatgat cagcttgtaa gccttcgccc
    70921 actgatcgaa aattattttg atcagacgat ggtcatggca gaagaaccag cagtacgtaa
    70981 gaaccgatta acgcaattaa gtcaaattgc tgccatggct ttgtcactag caagtttaga
    71041 tcaaatcatc acaaaataat ttctaatatg taaataaacc agagaacagg cacaaaatag
    71101 gctatttctc tggtttattt gttataaata taaataacta ttattaattc aatgttaatt
    71161 tttttgttat tttatttttt gtgtaacttt taaaaacacc tatgaaa
//
LOCUS       DAYYKC010000004        64887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000004 DAYYKC010000000
VERSION     DAYYKC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.004"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1421)
                     /locus_tag="ACHBBW_000273"
     CDS             complement(<1..1421)
                     /locus_tag="ACHBBW_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM9151987.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="ACHBBW_000274"
     CDS             1673..2854
                     /locus_tag="ACHBBW_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9151988.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHBBW_000275"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHBBW_000275"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9151989.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="ACHBBW_000276"
     CDS             complement(4993..7704)
                     /locus_tag="ACHBBW_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9151990.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="ACHBBW_000277"
     CDS             complement(7714..8415)
                     /locus_tag="ACHBBW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9151991.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="ACHBBW_000278"
     CDS             8601..9200
                     /locus_tag="ACHBBW_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9151992.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="ACHBBW_000279"
     CDS             9281..10294
                     /locus_tag="ACHBBW_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9151993.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHBBW_000280"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHBBW_000280"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9151994.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHBBW_000281"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHBBW_000281"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9151995.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="ACHBBW_000282"
     CDS             11742..13115
                     /locus_tag="ACHBBW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9151996.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="ACHBBW_000283"
     CDS             13472..15616
                     /locus_tag="ACHBBW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9151997.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="ACHBBW_000284"
     CDS             complement(15668..16708)
                     /locus_tag="ACHBBW_000284"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9151998.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="ACHBBW_000285"
     CDS             complement(16985..18787)
                     /locus_tag="ACHBBW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9151999.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="ACHBBW_000286"
     CDS             complement(18801..19553)
                     /locus_tag="ACHBBW_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152000.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="ACHBBW_000287"
     CDS             complement(19562..19924)
                     /locus_tag="ACHBBW_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9152001.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="ACHBBW_000288"
     CDS             complement(20100..20972)
                     /locus_tag="ACHBBW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9152002.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="ACHBBW_000289"
     CDS             complement(21023..21511)
                     /locus_tag="ACHBBW_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9152003.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="ACHBBW_000290"
     CDS             complement(21649..22440)
                     /locus_tag="ACHBBW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9152004.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="ACHBBW_000291"
     CDS             complement(22495..23103)
                     /locus_tag="ACHBBW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9152005.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="ACHBBW_000292"
     CDS             complement(23096..23413)
                     /locus_tag="ACHBBW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9152006.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="ACHBBW_000293"
     CDS             complement(23569..24297)
                     /locus_tag="ACHBBW_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9152007.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="ACHBBW_000294"
     CDS             24456..25337
                     /locus_tag="ACHBBW_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9152008.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26616)
                     /locus_tag="ACHBBW_000295"
                     /pseudo
     CDS             complement(25574..26616)
                     /locus_tag="ACHBBW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(26619..27287)
                     /locus_tag="ACHBBW_000296"
     CDS             complement(26619..27287)
                     /locus_tag="ACHBBW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9152009.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27429..29765
                     /locus_tag="ACHBBW_000297"
     CDS             27429..29765
                     /locus_tag="ACHBBW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9152010.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29880..30191)
                     /locus_tag="ACHBBW_000298"
     CDS             complement(29880..30191)
                     /locus_tag="ACHBBW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152011.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30372..31847
                     /locus_tag="ACHBBW_000299"
     CDS             30372..31847
                     /locus_tag="ACHBBW_000299"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9152012.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31894..32478
                     /locus_tag="ACHBBW_000300"
     CDS             31894..32478
                     /locus_tag="ACHBBW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9152013.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32480..32863
                     /locus_tag="ACHBBW_000301"
     CDS             32480..32863
                     /locus_tag="ACHBBW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9152014.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33180..33464)
                     /locus_tag="ACHBBW_000302"
     CDS             complement(33180..33464)
                     /locus_tag="ACHBBW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9152015.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33816..34526)
                     /locus_tag="ACHBBW_000303"
     CDS             complement(33816..34526)
                     /locus_tag="ACHBBW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9152016.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34523..35995)
                     /locus_tag="ACHBBW_000304"
     CDS             complement(34523..35995)
                     /locus_tag="ACHBBW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9152017.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36133..36606
                     /locus_tag="ACHBBW_000305"
     CDS             36133..36606
                     /locus_tag="ACHBBW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9152018.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36759..37310)
                     /locus_tag="ACHBBW_000306"
     CDS             complement(36759..37310)
                     /locus_tag="ACHBBW_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9152019.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37415..37945
                     /locus_tag="ACHBBW_000307"
     CDS             37415..37945
                     /locus_tag="ACHBBW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9152020.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38428..38871)
                     /locus_tag="ACHBBW_000308"
     CDS             complement(38428..38871)
                     /locus_tag="ACHBBW_000308"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152021.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38887..40047)
                     /locus_tag="ACHBBW_000309"
     CDS             complement(38887..40047)
                     /locus_tag="ACHBBW_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9152022.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40554..42254
                     /locus_tag="ACHBBW_000310"
     CDS             40554..42254
                     /locus_tag="ACHBBW_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152023.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42356..43189)
                     /locus_tag="ACHBBW_000311"
     CDS             complement(42356..43189)
                     /locus_tag="ACHBBW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9152024.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43493..45658)
                     /locus_tag="ACHBBW_000312"
     CDS             complement(43493..45658)
                     /locus_tag="ACHBBW_000312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9152025.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45918..48212
                     /locus_tag="ACHBBW_000313"
     CDS             45918..48212
                     /locus_tag="ACHBBW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9152026.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHBBW_000314"
     CDS             48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHBBW_000314"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9152027.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48863..49870
                     /locus_tag="ACHBBW_000315"
     CDS             48863..49870
                     /locus_tag="ACHBBW_000315"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9152028.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49892..50911
                     /locus_tag="ACHBBW_000316"
     CDS             49892..50911
                     /locus_tag="ACHBBW_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9152029.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHBBW_000317"
     CDS             complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHBBW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9152030.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHBBW_000318"
     CDS             complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHBBW_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9152031.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHBBW_000319"
     CDS             complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHBBW_000319"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9152032.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHBBW_000320"
     CDS             complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHBBW_000320"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9152033.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57868..58073)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58336..59637
                     /locus_tag="ACHBBW_000321"
     CDS             58336..59637
                     /locus_tag="ACHBBW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9152034.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59706..60305)
                     /locus_tag="ACHBBW_000322"
     CDS             complement(59706..60305)
                     /locus_tag="ACHBBW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152035.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60559..61440
                     /locus_tag="ACHBBW_000323"
     CDS             60559..61440
                     /locus_tag="ACHBBW_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9152036.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61453..63063
                     /locus_tag="ACHBBW_000324"
     CDS             61453..63063
                     /locus_tag="ACHBBW_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152037.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63140..63799)
                     /locus_tag="ACHBBW_000325"
     CDS             complement(63140..63799)
                     /locus_tag="ACHBBW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9152038.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64082..64747)
                     /locus_tag="ACHBBW_000326"
     CDS             complement(64082..64747)
                     /locus_tag="ACHBBW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9152039.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19457 a        13218 c        11704 g        20508 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaacgat
    25981 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26041 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26101 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26161 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26221 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26281 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26341 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26401 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26461 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26521 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26581 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26641 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26701 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26761 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26821 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26881 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    26941 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27001 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27061 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27121 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27181 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27241 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27301 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27361 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27421 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27481 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27541 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27601 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27661 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27721 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27781 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa
    27841 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27901 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    27961 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28021 ataatcctga actggcccat caatacgcag atcgaatcat tgaattttct gatggtaaga
    28081 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28141 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28201 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28261 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28321 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28381 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28441 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28501 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28561 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28621 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28681 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28741 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28801 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28861 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28921 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    28981 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29041 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29101 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29161 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29221 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29281 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29341 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29401 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29461 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29521 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29581 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29641 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29701 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29761 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29821 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29881 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    29941 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30001 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30061 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30121 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc
    30181 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30241 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30301 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30361 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30421 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30481 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30541 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30601 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30661 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30721 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30781 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30841 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30901 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    30961 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31021 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31081 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31141 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31201 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31261 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31321 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31381 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31441 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31501 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31561 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31621 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31681 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31741 taagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31801 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31861 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31921 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    31981 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32041 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32101 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32161 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32221 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32281 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32341 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32401 gttaatcacc atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa
    32461 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32521 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32581 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32641 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32701 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32761 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32821 gattttagat tggttggaaa aaatagattt ccatatcaaa taaccagttt attataaaga
    32881 tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    32941 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33001 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33061 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33121 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33181 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33241 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33301 ttcacgatac tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca
    33361 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact
    33421 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33481 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33541 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33601 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33661 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33721 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33781 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33841 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33901 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    33961 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34021 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34081 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34141 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34201 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34261 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34321 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34381 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34441 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34501 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34561 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34621 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34681 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34741 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34801 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34861 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34921 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    34981 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35041 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35101 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35161 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35221 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35281 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35341 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35401 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35461 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35521 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35581 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac
    35641 cgcaagcttg gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35701 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35761 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35821 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35881 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    35941 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36001 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36061 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36121 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36181 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36241 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36301 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36361 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36421 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36481 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36541 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36601 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36661 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36721 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36781 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36841 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36901 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    36961 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37021 ttaaccggta gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct
    37081 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37141 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37201 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37261 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37321 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37381 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37441 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37501 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37561 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37621 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37681 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37741 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37801 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37861 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37921 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    37981 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38041 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38101 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38161 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38221 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38281 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38341 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38401 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38461 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38521 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38581 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38641 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38701 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38761 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38821 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38881 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    38941 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39001 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39061 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39121 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39181 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39241 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39301 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39361 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39421 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39481 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39541 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39601 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39661 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39721 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39781 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39841 gtttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39901 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    39961 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40021 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40081 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40141 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40201 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40261 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct
    40321 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40381 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40441 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40501 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40561 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40621 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40681 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40741 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40801 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40861 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40921 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    40981 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41041 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41101 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41161 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41221 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41281 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41341 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41401 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41461 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41521 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41581 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41641 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41701 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41761 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41821 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41881 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    41941 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42001 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42061 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa
    42121 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42181 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42241 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42301 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42361 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42421 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42481 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42541 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42601 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42661 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42721 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42781 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa
    42841 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42901 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    42961 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga
    43021 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43081 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43141 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43201 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43261 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43321 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43381 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43441 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43501 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43561 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43621 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43681 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43741 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43801 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43861 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43921 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    43981 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44041 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44101 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44161 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44221 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44281 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44341 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44401 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44461 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44521 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44581 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44641 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44701 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44761 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44821 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44881 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    44941 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45001 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45061 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45121 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45181 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45241 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45301 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45361 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45421 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45481 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45541 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45601 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45661 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45721 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45781 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45841 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45901 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    45961 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46021 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46081 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46141 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46201 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46261 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46321 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46381 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46441 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46501 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46561 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46621 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46681 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46741 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46801 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46861 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46921 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    46981 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47041 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47101 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47161 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47221 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47281 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47341 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47401 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47461 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47521 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47581 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47641 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47701 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47761 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47821 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47881 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    47941 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48001 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48061 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48121 gttctcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48181 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48241 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48301 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48361 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48421 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48481 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48541 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48601 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48661 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48721 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48781 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48841 ttgaaagaaa agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48901 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    48961 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49021 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49081 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49141 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49201 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49261 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49321 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49381 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49441 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49501 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49561 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49621 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49681 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49741 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49801 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49861 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49921 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    49981 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50041 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50101 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50161 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50221 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50281 ttcagatagt caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50341 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50401 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50461 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50521 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50581 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50641 cttcgccctt cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact
    50701 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50761 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50821 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50881 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    50941 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51001 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51061 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51121 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51181 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51241 aagacaggtg caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc
    51301 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51361 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51421 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51481 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51541 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51601 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51661 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51721 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51781 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51841 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51901 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    51961 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52021 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52081 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52141 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52201 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52261 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52321 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52381 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52441 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52501 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52561 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52621 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct
    52681 aaactttttt ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga
    52741 ataacgaaca tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta
    52801 cctaatgtag caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa
    52861 gctaatccgt ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta
    52921 cgcattttaa gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta
    52981 gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca
    53041 aataatgaaa taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt
    53101 tctttatgga atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt
    53161 tttgatggtc cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag
    53221 ctgaagatat ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc
    53281 tgcgtgatat ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca
    53341 ttgatgacat tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt
    53401 tttgttgcgt ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata
    53461 aatgtgatac cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt
    53521 gaccatgaaa agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa
    53581 acacctaaaa cgaacatgat gactactggg atatataccc ctagccatac accgatgttc
    53641 ccgccgactt ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact
    53701 aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt
    53761 cctaacaatg gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat
    53821 aatagccatg caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga
    53881 ggaccgcctt cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg
    53941 gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac
    54001 gtaggaatac tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga
    54061 cccattttac ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa
    54121 ataacggtgt ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg
    54181 ggattctttg agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat
    54241 cgaagagctt aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta
    54301 ttcaagctgt tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt
    54361 ctgcgtagat tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt
    54421 caaagtgttc ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt
    54481 ttccggcacg gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc
    54541 tgttgccata gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa
    54601 tgcttccttt cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa
    54661 ctaagtcatc gttgcctttt tctttgaata catagtctac catattgaaa tctggataag
    54721 ttaatggatg aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt
    54781 tgtagaaacg atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat
    54841 tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac
    54901 cttccaagat ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg
    54961 tcgcaaagta agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag
    55021 aatatggaac atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg
    55081 ctttatatgc actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt
    55141 atttgtaatg aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga
    55201 aaatcgcacg gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt
    55261 ctttttctaa tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt
    55321 ctgttgatcc tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac
    55381 gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa
    55441 tcacttggtc taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag
    55501 tttgtggaac caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag
    55561 ctttgatttc gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg
    55621 acaagttcat taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca
    55681 ttgcaagcgg caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag
    55741 aaccatcagc aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca
    55801 ttcctacttc ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac
    55861 cattccatag catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca
    55921 tatgacccca gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg
    55981 aaatttctga tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt
    56041 tttcttctgg cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat
    56101 gttcatccac caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc
    56161 caatgaatag ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct
    56221 cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa
    56281 actaagtttt ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc
    56341 acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag
    56401 attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta
    56461 atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa
    56521 aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg
    56581 atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga
    56641 aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac
    56701 gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat
    56761 ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc
    56821 ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg
    56881 tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg
    56941 agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca
    57001 gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc
    57061 agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc
    57121 ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca
    57181 ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc
    57241 gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct
    57301 ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa
    57361 aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc
    57421 atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg
    57481 ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt
    57541 tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat
    57601 aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag
    57661 tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat
    57721 ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt
    57781 ttatgaaagc aggaacctgc tagcatatgt aattaattat ttcttcagga taggatagga
    57841 aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt
    57901 tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact
    57961 gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca
    58021 gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta
    58081 atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt
    58141 aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta
    58201 ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac
    58261 tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag
    58321 caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta
    58381 actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa
    58441 ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa
    58501 caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg
    58561 ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta
    58621 aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt
    58681 ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac
    58741 aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat
    58801 gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc
    58861 tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc
    58921 agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac
    58981 aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg
    59041 gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa
    59101 ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa
    59161 tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag
    59221 ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa
    59281 gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc
    59341 ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct
    59401 tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt
    59461 aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct
    59521 cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt
    59581 gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt
    59641 tacgtcattt atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt
    59701 atgctttatt tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta
    59761 tatggaagca gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa
    59821 tagaggatca agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga
    59881 tggaaaagat caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa
    59941 aattcttttt tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc
    60001 actcgattga gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga
    60061 agattttccg caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat
    60121 acttcataaa tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc
    60181 tgagcgttct cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc
    60241 aagtcaaaag ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg
    60301 ttcatttgta gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt
    60361 ctgaggttta ttataactat ctattgagaa agtatcaagc tagcaattcc ttgtttatga
    60421 attcattgac agaaatttat aaaaagaata ttatgtacta aaataataaa aaaagtttta
    60481 taaaaaggta taaaaccttt ttattaaact atttaatctt gtttagtata tatttttaga
    60541 aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt
    60601 ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac attcaggtga agtcgttgga
    60661 tttatcggac caaatggttc tggaaaatcc acgacgattc ggacactttt agggattata
    60721 aaaaaagacg gcggaacggc aacgattttt gggcaagatg tatgggaaga tagtttatct
    60781 atccatgaac gtctttctta tgttccaggc gatatctctt tgtggggaaa tttgactgga
    60841 ggagcgatca tcgatctctt catgaagctt catggaaaag ggagtgctca aaaaaaagat
    60901 gaactgattc aacgtttctc tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga
    60961 aatcgccaaa aagtgggctt gattgctgct ttggctgttg attcggatct ctatttattt
    61021 gatgagccta cttctggttt agatcctttg atggaaacga ttttccaaga agaagttgaa
    61081 aaattgaaag aacagggcaa atccatccta ctttcttctc atattctcag tgaagtcgag
    61141 cgactagcgg ataaagtcgt gatcatacgc gaaggaacac tcgatgaaga aggaacactc
    61201 gatgaattgc gtcatttgac tcgttcgacc atcactatag aaacagaagc agagaatagt
    61261 cacttagcac aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt
    61321 gctgctgata acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa
    61381 aaatttgaat ctattccacc gactcttgag gatttattca tgagacatta tgaaggatga
    61441 ggaggaagcg aaatgactga taaattttca cgctggtcca cccttttttc tcaaaacctt
    61501 agccgtgact ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt ttctggtggt
    61561 tttgtccctg cgtttgaaga attgacttcc ggacaaggat tagctggatt atacgaaacc
    61621 ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag
    61681 tatactttag gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct
    61741 gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa aagaagaact tggtctgatt
    61801 gaacttattc gttcctttcg agtcggacgt caagcaaact ctttagctgt catcgctgaa
    61861 agtattctga tcaatttgct tttagcaata tcgatcagct gtacgatgat ttttttccaa
    61921 gcggattcga tcactgcaga aggtgcttgg ctttttgggc tatctgtagg tagtatgggg
    61981 attttgggga gtgccgttgc actgtttgct gcacaactga tgccaagttc agccagttcg
    62041 aatggtctgt ctttagcgtt tttaggtgga ctttaccttc tacgagcaat aacagatgta
    62101 gctgattcct ccctttcgat gtggaatcct ttaggctggt cttatttgac ctatccattt
    62161 acaactaaca actggattcc tttgctttat ttgtttttgt tcagtctaat cttgataatc
    62221 agttcatttg ttttagagga acatcgagat ttgaacaccg gctatatccc cgaacgtgaa
    62281 ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg gtttattatt ctatctaaat
    62341 aaagggatga caatcagttg gcttatcact tttggtttga tgggggcggc atacggctct
    62401 atctacggga atatgcaaca gtttttagaa agtaacgact taatgaaaca gatgtttatc
    62461 caatctggcg tctcaattga aaaaaacttt acaagcacga tcatggtagt cttgatttgt
    62521 ctagctgcta ttttaccaat tgctagcatc aatcgtttat ttacagaaga aacccacgga
    62581 cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc aactttattg gaccaatatg
    62641 ttactagcta ttctatttgc tatattggca atcctgatta gttctggtgc actagggctt
    62701 tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc
    62761 gggttcaatt tcctgcctgc cgttttagtg tttatcggaa ttagtggctt tctattcgga
    62821 tggcttcctc gaatcggtaa acttgcttat atctacttag cttactcctt tgcactcaat
    62881 tactttggcg gaattttgga tttaccagaa tggttttcca aaacagctgt ccctagctgg
    62941 gttccacgga tgccaaacga ttcttttgat ctttccgtgt tccttacgtt atcatgtatc
    63001 agccttgcac tgttcgtcct tggttctatt ggttataggc gaagagactt actagaagga
    63061 taataaaaaa gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa aatccaacat
    63121 ttggaggtca tttttttatt tacaagtctt ctaactgctc tttatcaatg atccagactt
    63181 ctcgattctt tctttttaca taaccattct cctctaataa tttaaatttt ctagataaag
    63241 tttcgggggt cgtaccgata aatgcagcaa gttctttcat tttcattggc aggaaaaagt
    63301 gatccttccc tgtcgcttta gctaagtcaa gcagatagag agcgactcgt tcttctactt
    63361 tttccatcat caaaaaagtt gtctgctgct gtacttttgt taatttctca acgttgattg
    63421 ctaaaagttt cagactaagg gcgggataat gcagtaagag ttccgagaaa tctttttgtc
    63481 tcaatatgca aatttccgtt ttttctagtg cttcgccaaa taaagactca tttggaacgc
    63541 ctagcaactg gctctctccc tcatatccgc ccggctctat tactcgtaaa agctgttcct
    63601 ttccgctagc agataattga tacactttca tactgccgga ggccacgatc actaattgag
    63661 gttctccatt cggcgcaaga atctgttctc ctttttccac atagtgatgt ttgacaagtg
    63721 cgtgtacttt ttcttgatct tgctgtacta agtgattgaa caaaggaaca aatgaaacac
    63781 ataaatgatc atgactcata atctctcctc cttctaaaca gctatttcta ataaagaaac
    63841 ataacttccc ttattccaac tgataatttt cttttatcat agcataaacg tcaagcgaac
    63901 cagataccag caaatagagt ggatttcttt ttgtcccttg ctttattttc cttcttttga
    63961 gaccttaagt aattgattcc tatcaatttt ttaaatattt actttttgaa taatcaaaaa
    64021 caaaccttca ctcgcaagcc gagagtaagg tttgtttttc gcattgctta tttcgtgttg
    64081 tctattgaaa tttttccgca cctagctttt gatatagtgg tgctagttct tgtaaggttt
    64141 cagcggttat ttgctcaacg tgttcgattt cttgcggata gaagaatagt ttacccattt
    64201 gaaaggttcg gtctctcatc gcttttttca actcttcttg tttctttgta tcggacaaat
    64261 agtcagacaa agctaagtgg tcgtcaagtt catgttctgg ttgcggccat atgtaataat
    64321 ccttgatatc gactgttttg ctccactctt tcagtatcgc aagcaactgg ttatacgctg
    64381 caatgctacc atatcgttct tcgcttttat aatgttggta catcaaatag atttggtatt
    64441 gtttttgatt caacaaaact gctagacagg cattctcgtc ttgacgagat tcgcttcggt
    64501 acgcgctcca aaaatgactg cgtaaattcc agccattcgt ccagctttcg attttaggtt
    64561 tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg gagctgaatc tctttccatt
    64621 tttgccagtg ttctttatat tctttcttga tttgttcaat cttatctgtt ggatggctct
    64681 ctttgagctg tttgaatgcg tagattggtc gatcaaacaa ctgataatct gattttccaa
    64741 gttccattgt ttcgcctctt tccgttatct tttgtattct acctgaattt tgcgatatgg
    64801 acaaatagag gttgtgaaaa agctgttctg catcaagtca taagaacagc caaacaaaaa
    64861 actgcgatga gcatcaccgc agtttta
//
LOCUS       DAYYKC010000005        58332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000005 DAYYKC010000000
VERSION     DAYYKC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.005"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..919)
                     /locus_tag="ACHBBW_000327"
     CDS             complement(71..919)
                     /locus_tag="ACHBBW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9152040.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1143..3083)
                     /gene="thrS"
                     /locus_tag="ACHBBW_000328"
     CDS             complement(1143..3083)
                     /gene="thrS"
                     /locus_tag="ACHBBW_000328"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9152041.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3420..3599)
                     /locus_tag="ACHBBW_000329"
     CDS             complement(3420..3599)
                     /locus_tag="ACHBBW_000329"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9152042.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3646..5469)
                     /locus_tag="ACHBBW_000330"
     CDS             complement(3646..5469)
                     /locus_tag="ACHBBW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9152043.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5747..8026
                     /gene="helD"
                     /locus_tag="ACHBBW_000331"
     CDS             5747..8026
                     /gene="helD"
                     /locus_tag="ACHBBW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9152044.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8119..8766)
                     /locus_tag="ACHBBW_000332"
     CDS             complement(8119..8766)
                     /locus_tag="ACHBBW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152045.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8945..9664)
                     /locus_tag="ACHBBW_000333"
     CDS             complement(8945..9664)
                     /locus_tag="ACHBBW_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9152046.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9795..10946)
                     /locus_tag="ACHBBW_000334"
     CDS             complement(9795..10946)
                     /locus_tag="ACHBBW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9152047.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11163..11903)
                     /locus_tag="ACHBBW_000335"
     CDS             complement(11163..11903)
                     /locus_tag="ACHBBW_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9152048.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11907..12230)
                     /gene="rsfS"
                     /locus_tag="ACHBBW_000336"
     CDS             complement(11907..12230)
                     /gene="rsfS"
                     /locus_tag="ACHBBW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9152049.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12287..12880)
                     /gene="yqeK"
                     /locus_tag="ACHBBW_000337"
     CDS             complement(12287..12880)
                     /gene="yqeK"
                     /locus_tag="ACHBBW_000337"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9152050.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12864..13514)
                     /locus_tag="ACHBBW_000338"
     CDS             complement(12864..13514)
                     /locus_tag="ACHBBW_000338"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9152051.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13524..13835)
                     /gene="yhbY"
                     /locus_tag="ACHBBW_000339"
     CDS             complement(13524..13835)
                     /gene="yhbY"
                     /locus_tag="ACHBBW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9152052.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13851..14960)
                     /gene="yqeH"
                     /locus_tag="ACHBBW_000340"
     CDS             complement(13851..14960)
                     /gene="yqeH"
                     /locus_tag="ACHBBW_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9152053.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14957..15484)
                     /locus_tag="ACHBBW_000341"
     CDS             complement(14957..15484)
                     /locus_tag="ACHBBW_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9152054.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15664..16449)
                     /locus_tag="ACHBBW_000342"
     CDS             complement(15664..16449)
                     /locus_tag="ACHBBW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9152055.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16442..17311)
                     /gene="accD"
                     /locus_tag="ACHBBW_000343"
     CDS             complement(16442..17311)
                     /gene="accD"
                     /locus_tag="ACHBBW_000343"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9152056.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17312..18691)
                     /locus_tag="ACHBBW_000344"
     CDS             complement(17312..18691)
                     /locus_tag="ACHBBW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9152057.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18699..19118)
                     /gene="fabZ"
                     /locus_tag="ACHBBW_000345"
     CDS             complement(18699..19118)
                     /gene="fabZ"
                     /locus_tag="ACHBBW_000345"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9152058.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19143..19619)
                     /gene="accB"
                     /locus_tag="ACHBBW_000346"
     CDS             complement(19143..19619)
                     /gene="accB"
                     /locus_tag="ACHBBW_000346"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9152059.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19622..20857)
                     /gene="fabF"
                     /locus_tag="ACHBBW_000347"
     CDS             complement(19622..20857)
                     /gene="fabF"
                     /locus_tag="ACHBBW_000347"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9152060.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20876..21613)
                     /gene="fabG"
                     /locus_tag="ACHBBW_000348"
     CDS             complement(20876..21613)
                     /gene="fabG"
                     /locus_tag="ACHBBW_000348"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9152061.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21625..22542)
                     /gene="fabD"
                     /locus_tag="ACHBBW_000349"
     CDS             complement(21625..22542)
                     /gene="fabD"
                     /locus_tag="ACHBBW_000349"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9152062.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22649..22873)
                     /locus_tag="ACHBBW_000350"
     CDS             complement(22649..22873)
                     /locus_tag="ACHBBW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9152063.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22926..23888)
                     /locus_tag="ACHBBW_000351"
     CDS             complement(22926..23888)
                     /locus_tag="ACHBBW_000351"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9152064.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23898..24338)
                     /locus_tag="ACHBBW_000352"
     CDS             complement(23898..24338)
                     /locus_tag="ACHBBW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9152065.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24644..25609
                     /locus_tag="ACHBBW_000353"
     CDS             24644..25609
                     /locus_tag="ACHBBW_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9152066.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25666..25896)
                     /locus_tag="ACHBBW_000354"
     CDS             complement(25666..25896)
                     /locus_tag="ACHBBW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9152067.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25982..26869)
                     /locus_tag="ACHBBW_000355"
     CDS             complement(25982..26869)
                     /locus_tag="ACHBBW_000355"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9152068.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26958..28181)
                     /locus_tag="ACHBBW_000356"
     CDS             complement(26958..28181)
                     /locus_tag="ACHBBW_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9152069.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28198..29244)
                     /locus_tag="ACHBBW_000357"
     CDS             complement(28198..29244)
                     /locus_tag="ACHBBW_000357"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9152070.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29408..31135)
                     /gene="ptsP"
                     /locus_tag="ACHBBW_000358"
     CDS             complement(29408..31135)
                     /gene="ptsP"
                     /locus_tag="ACHBBW_000358"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9152071.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31135..31401)
                     /locus_tag="ACHBBW_000359"
     CDS             complement(31135..31401)
                     /locus_tag="ACHBBW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9152072.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31880..34114
                     /locus_tag="ACHBBW_000360"
     CDS             31880..34114
                     /locus_tag="ACHBBW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9152073.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34262..34582
                     /locus_tag="ACHBBW_000361"
     CDS             34262..34582
                     /locus_tag="ACHBBW_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9152074.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34787..35011)
                     /locus_tag="ACHBBW_000362"
     CDS             complement(34787..35011)
                     /locus_tag="ACHBBW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM9152075.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35209..36789)
                     /locus_tag="ACHBBW_000363"
     CDS             complement(35209..36789)
                     /locus_tag="ACHBBW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9152076.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37190..38566)
                     /locus_tag="ACHBBW_000364"
     CDS             complement(37190..38566)
                     /locus_tag="ACHBBW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9152077.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38694..39782)
                     /locus_tag="ACHBBW_000365"
     CDS             complement(38694..39782)
                     /locus_tag="ACHBBW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9152078.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39954..40388
                     /locus_tag="ACHBBW_000366"
     CDS             39954..40388
                     /locus_tag="ACHBBW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152079.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40466..40999)
                     /locus_tag="ACHBBW_000367"
     CDS             complement(40466..40999)
                     /locus_tag="ACHBBW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9152080.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41093..42271)
                     /gene="mutY"
                     /locus_tag="ACHBBW_000368"
     CDS             complement(41093..42271)
                     /gene="mutY"
                     /locus_tag="ACHBBW_000368"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9152081.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42264..43061)
                     /gene="recX"
                     /locus_tag="ACHBBW_000369"
     CDS             complement(42264..43061)
                     /gene="recX"
                     /locus_tag="ACHBBW_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9152082.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43168..44550
                     /gene="rlmD"
                     /locus_tag="ACHBBW_000370"
     CDS             43168..44550
                     /gene="rlmD"
                     /locus_tag="ACHBBW_000370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9152083.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44724..45302)
                     /locus_tag="ACHBBW_000371"
     CDS             complement(44724..45302)
                     /locus_tag="ACHBBW_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9152084.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45624..46094
                     /locus_tag="ACHBBW_000372"
     CDS             45624..46094
                     /locus_tag="ACHBBW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9152085.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46202..47428)
                     /locus_tag="ACHBBW_000373"
     CDS             complement(46202..47428)
                     /locus_tag="ACHBBW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9152086.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48182..48382)
                     /locus_tag="ACHBBW_000374"
     CDS             complement(48182..48382)
                     /locus_tag="ACHBBW_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9152087.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48516..49124)
                     /locus_tag="ACHBBW_000375"
     CDS             complement(48516..49124)
                     /locus_tag="ACHBBW_000375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9152088.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49209..49679)
                     /locus_tag="ACHBBW_000376"
     CDS             complement(49209..49679)
                     /locus_tag="ACHBBW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9152089.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49727..50641)
                     /locus_tag="ACHBBW_000377"
     CDS             complement(49727..50641)
                     /locus_tag="ACHBBW_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9152090.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50787..51590
                     /locus_tag="ACHBBW_000378"
     CDS             50787..51590
                     /locus_tag="ACHBBW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9152091.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51768..52715
                     /locus_tag="ACHBBW_000379"
     CDS             51768..52715
                     /locus_tag="ACHBBW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9152092.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52783..53076)
                     /locus_tag="ACHBBW_000380"
     CDS             complement(52783..53076)
                     /locus_tag="ACHBBW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9152093.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(53104..53769)
                     /locus_tag="ACHBBW_000381"
     CDS             complement(53104..53769)
                     /locus_tag="ACHBBW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9152094.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53909..54541)
                     /locus_tag="ACHBBW_000382"
     CDS             complement(53909..54541)
                     /locus_tag="ACHBBW_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9152095.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54548..55615)
                     /gene="liaS"
                     /locus_tag="ACHBBW_000383"
     CDS             complement(54548..55615)
                     /gene="liaS"
                     /locus_tag="ACHBBW_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9152096.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55612..56343)
                     /gene="liaF"
                     /locus_tag="ACHBBW_000384"
     CDS             complement(55612..56343)
                     /gene="liaF"
                     /locus_tag="ACHBBW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9152097.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56512..56991)
                     /gene="greA"
                     /locus_tag="ACHBBW_000385"
     CDS             complement(56512..56991)
                     /gene="greA"
                     /locus_tag="ACHBBW_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9152098.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57127..58302)
                     /gene="mltG"
                     /locus_tag="ACHBBW_000386"
     CDS             complement(57127..58302)
                     /gene="mltG"
                     /locus_tag="ACHBBW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9152099.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        16809 a        12309 c         9863 g        19351 t
ORIGIN      
        1 atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat
       61 agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca
      121 ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag
      181 caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag
      241 atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg
      301 caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc
      361 tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga
      421 gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca
      481 gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg
      541 attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt
      601 ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg
      661 cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt
      721 tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac
      781 tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac
      841 cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa
      901 tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac
      961 catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca
     1021 acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa
     1081 tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc
     1141 tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc
     1201 aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc
     1261 aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc
     1321 ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa
     1381 cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt
     1441 gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg
     1501 acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt
     1561 tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt
     1621 atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt
     1681 ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg
     1741 gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta
     1801 ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat
     1861 actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca
     1921 aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct
     1981 gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa
     2041 gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca
     2101 agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata atgatcccag
     2161 tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa
     2221 cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct
     2281 tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt
     2341 tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct
     2401 ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt
     2461 gagtttccac gccaataagc acctgccact gataataatt taaatacttg tatacggcca
     2521 gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta
     2581 atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa
     2641 atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg
     2701 attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca
     2761 ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt
     2821 ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca
     2881 agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga
     2941 gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt
     3001 gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca
     3061 tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat
     3121 catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa
     3181 gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg
     3241 accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa
     3301 gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc
     3361 aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact
     3421 tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca
     3481 tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc
     3541 atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata
     3601 atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt
     3661 cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat
     3721 agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt
     3781 acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc
     3841 tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt
     3901 caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat
     3961 accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac
     4021 aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg
     4081 ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc
     4141 cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc
     4201 tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt
     4261 tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc
     4321 aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag
     4381 ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat
     4441 atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc
     4501 attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc
     4561 aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat
     4621 cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag
     4681 tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca
     4741 ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc
     4801 taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg
     4861 atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa
     4921 agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa
     4981 agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc
     5041 gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa
     5101 ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc
     5161 tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa
     5221 tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc
     5281 taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa
     5341 gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa
     5401 aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa
     5461 atgtgtcatt gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca
     5521 gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta
     5581 attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata
     5641 ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat
     5701 gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca
     5761 actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct
     5821 ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa
     5881 tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca
     5941 acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca
     6001 aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg
     6061 tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat
     6121 cgttattgac tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc
     6181 attttatgaa actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa
     6241 aatccaagaa ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt
     6301 tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat
     6361 acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg
     6421 aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca
     6481 taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc
     6541 tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt
     6601 caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga
     6661 aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt
     6721 agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga
     6781 tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga
     6841 aacaaatgaa caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa
     6901 aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga
     6961 agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga
     7021 gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca
     7081 tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca
     7141 aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca
     7201 tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact
     7261 attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat
     7321 ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt
     7381 cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac
     7441 gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac
     7501 gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga
     7561 acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac
     7621 agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg
     7681 gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca
     7741 actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa
     7801 gcgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt
     7861 cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac
     7921 catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt
     7981 gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa aaagacaact
     8041 gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag aaaaagtaat
     8101 tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag gatattttaa
     8161 tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt ctgggtcaaa
     8221 taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc gattccattc
     8281 aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat ccaaaatctc
     8341 tgatacacga aatgctaggt atgcactatc gatcacttta ttttttttcg tgtctatctt
     8401 ctcatttctt tcgataagaa ttttttcagg acgatgtttc aagctccact ctaatagtag
     8461 aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat cagaacgacg
     8521 aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc ggtccaccat
     8581 acactctaaa taacggttca gataccctgt accgacatag tttcctaatc gatagccttc
     8641 aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa
     8701 ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag tgatttttgc
     8761 ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat gatacttatg
     8821 ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa aagcaagaca
     8881 atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg ttaaaactgg
     8941 ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct agatcttcca
     9001 gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt acctcgtttt
     9061 gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct tttaatgctt
     9121 cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct tcttcttcca
     9181 ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg tcagcgtctt
     9241 ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct ccggaaacac
     9301 ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat
     9361 tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct tcatagcgca
     9421 attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg tcaataatat
     9481 gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt tggtttgcat
     9541 gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat tttgcataaa
     9601 tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc catttacgtc
     9661 ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg tcctgttcca
     9721 ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca ttcttctcat
     9781 ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc cggaatattg
     9841 cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg tgcctctttt
     9901 tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct tcttccagca
     9961 tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat ctcttgcttt
    10021 ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt atatcttttt
    10081 gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg
    10141 cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa agtagaagat
    10201 aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga acaagttgaa
    10261 agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc ttctaacgca
    10321 tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc atgatagcct
    10381 gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc attttccttt
    10441 gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat atcgggatac
    10501 agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc agacagttct
    10561 tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa actgctgtaa
    10621 tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag tgtttgcaag
    10681 atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc gacgggaagc
    10741 tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca tttatcgatg
    10801 atagccggtt cgcctctttg caaaaaattc ccgctcatta cagcaatgac gacctctgct
    10861 cctgttattt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa aggattgtac
    10921 tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg ataaaaaaga
    10981 aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat ataaactatt
    11041 ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt
    11101 tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag caggcaaaac
    11161 cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc tgcttcgtct
    11221 gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat tcttaaataa
    11281 ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa tcgaccagac
    11341 ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct
    11401 ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct atattctgga
    11461 aaaacctcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc attttcttct
    11521 aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt catatagcac
    11581 agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa atcgagcata
    11641 ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc
    11701 aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc aaaggcaacc
    11761 gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga
    11821 cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg
    11881 aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc gactagcgga
    11941 gcatctgacc atagtttttc cagattatag aaatttcgtt ctggcgcatg gaaaacatgt
    12001 acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc ttcaatacgt
    12061 tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat tgcgttgatt
    12121 tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga aacatttcga
    12181 acgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt
    12241 aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat ttttttgcta
    12301 cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc gcaagcaaat
    12361 gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg
    12421 ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct gctacataaa
    12481 tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg attgcatgaa
    12541 gaatctctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca acaagaccat
    12601 gccagattgc attattataa ttcaataact ccggatcaaa accgtctctt acgattacca
    12661 ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca gcaatactag
    12721 ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct tctactccaa
    12781 gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc aggttttctc
    12841 tagaataagc agtatattca ttactatagt ccatattcat acaacccttc attttgaata
    12901 taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt gatcttttga
    12961 cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac aggatatgga
    13021 gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga agtcagctca
    13081 tcgattttat accattttgg caaatattct accatatccc caccgataat aaagtaataa
    13141 tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta acttttccca
    13201 ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc
    13261 aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg aggtggctga
    13321 taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg ttcagccatc
    13381 actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac ttgctttttc
    13441 ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa tgtcttagct
    13501 tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag cctttgatac
    13561 ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg gacgatttcg
    13621 caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc tgtattttga
    13681 agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg aacgatcatt
    13741 gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg cgctttgcta
    13801 cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc ttaaatcaat
    13861 gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac tactggcgct
    13921 gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt cacagaaaac
    13981 tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg ctgtaataat
    14041 ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg atgaatattt
    14101 acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa atcaaatctt
    14161 gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt aggtttgatt
    14221 tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc catttgatgg
    14281 cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg aatctcgatt
    14341 ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac tcccgctgta
    14401 tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc gacgatatag
    14461 acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat ctcatgagat
    14521 ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta agcacgttcc
    14581 ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc cactttgttc
    14641 ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat tagtgaacca
    14701 ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttttgccc taattcattc
    14761 agtaagcgca aaaagtcgtc atccgtcaat gagacatctt gggtctcatt ataatggcgt
    14821 aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc caaagccgtt
    14881 tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc acagccgata
    14941 cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg tgttttgcta
    15001 atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc caactatctg
    15061 ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct ccacgtatat
    15121 cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc aattgtttct
    15181 ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa
    15241 atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca acaacaggga
    15301 ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca tctggattat
    15361 tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct agattcttta
    15421 attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg taatttgaaa
    15481 acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc tcaaccccgt
    15541 attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt cagaaaaagc
    15601 atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa
    15661 caactaatat tggcggaacc tcttgtatcg tttttccagt aatttatgct gatctaatcg
    15721 aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg
    15781 tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg
    15841 tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt
    15901 ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat
    15961 ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat
    16021 gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc
    16081 tccttcgccg cgctcttctg ctcctacacc gcaataagca cctgctgtat tgatcaaggt
    16141 aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc
    16201 ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg
    16261 aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc
    16321 gtcatctgca aataaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa
    16381 aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt
    16441 cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct
    16501 gtttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag
    16561 ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag
    16621 caaaaccgat caagctttgg ggttctgcta gaataatatc gccttccatt gcaaaactgg
    16681 cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc
    16741 tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt
    16801 gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc
    16861 gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat
    16921 taggatccat cactccaata atgaagggaa tcccctgaat ggttgcttta ccagtgagga
    16981 ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa
    17041 atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa
    17101 taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata
    17161 ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc
    17221 acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc
    17281 gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg
    17341 tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg
    17401 caggatgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa
    17461 attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga
    17521 ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac
    17581 tgtctactcg cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag
    17641 aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt
    17701 ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt
    17761 tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag
    17821 tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg
    17881 cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt
    17941 ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgattg ttgcgttgta
    18001 aagaacaatc tcgctcgcct aaatgaatga catgaccaaa tcgatcacct aagatctgaa
    18061 cttctatatg gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg
    18121 ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa
    18181 ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa
    18241 ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa
    18301 tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat
    18361 cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa
    18421 atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga
    18481 ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa
    18541 tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata
    18601 tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac
    18661 cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat
    18721 tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa
    18781 gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta
    18841 cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga
    18901 gaaagtaatg caactgctcc tgcttgagcc attgcttcaa caatcaatac ccctggcata
    18961 acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta
    19021 gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa
    19081 cgatgtggaa tgatttcttt tatttcttgt atgttcattg ttttccctct ttcctgcttt
    19141 gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct
    19201 acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc
    19261 gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct
    19321 ggtttgtcgg gcgctggttg cagataaacg attcccacga ttggcgaaac aacctcgatt
    19381 ccatcaaata ctgccgattc tttttctttc attatttctg gagcagttgt tgcttgttca
    19441 ggtatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg
    19501 cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat
    19561 tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa
    19621 attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat
    19681 tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa
    19741 tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca
    19801 gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt
    19861 tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg
    19921 cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt
    19981 ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagatgca ccagaaccat
    20041 ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca acgatttctc
    20101 catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacgcctg
    20161 ctccttcacc catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag
    20221 gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa
    20281 tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga
    20341 atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac
    20401 cttttgcacc tacacgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta
    20461 cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga
    20521 ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt
    20581 tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg
    20641 aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt
    20701 tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga
    20761 tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg
    20821 aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc
    20881 cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg
    20941 ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt
    21001 gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga
    21061 atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt
    21121 ttgttaatcc tactacgcca gctttactag ctgcataatt tgcttgacca atatttccaa
    21181 tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt
    21241 tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatccaga cattttttga
    21301 aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta
    21361 acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat
    21421 aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta
    21481 tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa
    21541 aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc
    21601 cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt
    21661 attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac
    21721 ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact
    21781 ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt
    21841 cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat
    21901 ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg
    21961 cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc
    22021 ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc
    22081 aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg
    22141 cgcattcatc actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc
    22201 gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa
    22261 tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt
    22321 caataagcga taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg
    22381 atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact
    22441 tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg
    22501 atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc
    22561 cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat
    22621 gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta
    22681 aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa
    22741 tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag
    22801 tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt
    22861 tttcgaatac cataataata tattcctccg atattgtttg ctttttattt ttataattta
    22921 acagtctaca actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat
    22981 attactttct gctgttttcc taaggtgatc tttccactag aaactgcttc atccaataag
    23041 atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct
    23101 tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa
    23161 agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt
    23221 acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct
    23281 tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta
    23341 ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc
    23401 ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta
    23461 gatagtgttt caccaccaat caccagtccc ttagttgagc cgcagcggat cattttttca
    23521 gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca
    23581 tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc
    23641 gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc
    23701 aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt
    23761 tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga
    23821 tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat
    23881 tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca
    23941 tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca
    24001 cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt
    24061 acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg
    24121 ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt
    24181 gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct
    24241 ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta
    24301 acaagataat catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt
    24361 ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt
    24421 aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg
    24481 ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt
    24541 atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc attatttcaa
    24601 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
    24661 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat
    24721 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
    24781 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
    24841 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
    24901 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
    24961 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
    25021 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
    25081 aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga cttttgaaca
    25141 gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac tgacttactt
    25201 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
    25261 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcagttttt
    25321 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
    25381 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg acgtttctcc
    25441 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
    25501 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtt
    25561 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
    25621 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
    25681 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
    25741 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
    25801 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
    25861 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
    25921 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
    25981 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
    26041 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
    26101 ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt aaatctttga
    26161 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
    26221 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
    26281 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
    26341 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
    26401 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
    26461 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
    26521 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
    26581 tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
    26641 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
    26701 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
    26761 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
    26821 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
    26881 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
    26941 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
    27001 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
    27061 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
    27121 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
    27181 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca
    27241 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
    27301 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
    27361 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
    27421 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
    27481 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc ataagaaatc
    27541 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
    27601 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
    27661 atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac acgttcactt
    27721 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
    27781 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
    27841 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
    27901 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
    27961 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
    28021 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
    28081 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
    28141 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
    28201 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
    28261 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
    28321 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
    28381 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
    28441 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
    28501 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
    28561 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
    28621 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
    28681 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
    28741 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
    28801 ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa tagcttgaat
    28861 cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
    28921 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
    28981 cgatctgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
    29041 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
    29101 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
    29161 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
    29221 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
    29281 ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga caaaaacgtt
    29341 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
    29401 acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc atatcgcatt
    29461 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
    29521 tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
    29581 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
    29641 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
    29701 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
    29761 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
    29821 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
    29881 ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
    29941 tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga agcaatgcac
    30001 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
    30061 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
    30121 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
    30181 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
    30241 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
    30301 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
    30361 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
    30421 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
    30481 tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct ggaatttcta
    30541 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
    30601 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
    30661 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
    30721 tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca ttatcttcca
    30781 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
    30841 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
    30901 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
    30961 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
    31021 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
    31081 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
    31141 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
    31201 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
    31261 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
    31321 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
    31381 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
    31441 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
    31501 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
    31561 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt
    31621 cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
    31681 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
    31741 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
    31801 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
    31861 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
    31921 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
    31981 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
    32041 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
    32101 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
    32161 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
    32221 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
    32281 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
    32341 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
    32401 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
    32461 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
    32521 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
    32581 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
    32641 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
    32701 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
    32761 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
    32821 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
    32881 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
    32941 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
    33001 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
    33061 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
    33121 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
    33181 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
    33241 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
    33301 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
    33361 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
    33421 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
    33481 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
    33541 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
    33601 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
    33661 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
    33721 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
    33781 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
    33841 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
    33901 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
    33961 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
    34021 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
    34081 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
    34141 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
    34201 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
    34261 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
    34321 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
    34381 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
    34441 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
    34501 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    34561 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
    34621 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa gcagaagcaa
    34681 ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg tgaattccag
    34741 cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt
    34801 tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt
    34861 taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt
    34921 cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt ttctgttttt
    34981 ttctttcacg tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat
    35041 gtacgcaaaa taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa
    35101 aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt
    35161 ttggctattc ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact
    35221 ttttaatttt acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa
    35281 taagaacaac ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt
    35341 ctcatccaaa tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat
    35401 tacttcggcg ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc
    35461 aacagctcca atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc
    35521 ttgaactaat tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt
    35581 cactttcata aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt
    35641 tccttcgaac aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc
    35701 gcctgctact gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct
    35761 taatttcagt tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc
    35821 agaacagatt cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat
    35881 cttgaatacg aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt
    35941 tttgtgtcca taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc
    36001 gaagttagtc aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc
    36061 ttgattaaat gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata
    36121 taaggaattt tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac
    36181 taatcgttca tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc
    36241 ataaagacct tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat
    36301 atctaatagc tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag
    36361 tttgttgata aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt
    36421 tgtctgtgct tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat
    36481 caaagtacga taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac
    36541 tcgtttccct tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt
    36601 ctcgatttcc atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc
    36661 ggcttgacgg atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc
    36721 atccggatgg gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg
    36781 attattcata acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag
    36841 attgtccgat ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc
    36901 agtctctttt tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca
    36961 agccaaaaat acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc
    37021 tttttcacaa cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg
    37081 aaaaagccac caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac
    37141 gtgttgcttg tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg
    37201 gctcgtcatc ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg
    37261 tttcttccgg atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt
    37321 cagaaaccag cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca
    37381 tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac
    37441 tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt
    37501 catcgatttt ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga
    37561 tttcttctaa aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata
    37621 aaatagccat ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg
    37681 tttcaggaac gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga
    37741 atttatgggc agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt
    37801 cttcgttata aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt
    37861 ccacagtgtc gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg
    37921 caactttcgt atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata
    37981 cgccttcgtt ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat
    38041 caaaagtcat cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga
    38101 agggcttagc tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt
    38161 ctgatttatt taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg
    38221 tcaaaatagc cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata
    38281 ccggagctaa ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca
    38341 aagtgattcc tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa
    38401 gcttttgaga tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg
    38461 aaacaacagc aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata
    38521 ataaaatctg cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc
    38581 cttataaaat taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa
    38641 atggtatcac aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag
    38701 gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata
    38761 taggaaatga tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat
    38821 ccggcaatat ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt
    38881 gatttcccaa ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca
    38941 accacacaac aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt
    39001 gctagtccta gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca
    39061 aataagaaag cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg
    39121 aataaacatt cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc
    39181 aataactcca caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact
    39241 agtttttctc cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg
    39301 gttgccgaag cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat
    39361 tgctgaatga ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact
    39421 tccttgaaca aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa
    39481 tcaggcatat tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt
    39541 aaaatccaaa taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt
    39601 ttgatttttg ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct
    39661 gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag
    39721 tttatttctg atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac
    39781 attagtttag actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt
    39841 atatattata ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac
    39901 tttttatttg ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag
    39961 ttgtacaaac aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc
    40021 aagtttttgt agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc
    40081 attgtatcca cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt
    40141 tgcctttaga aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac
    40201 accaagggtt aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct
    40261 ataatacgat tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat
    40321 atacagctta cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga
    40381 ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc
    40441 actggcttac tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc
    40501 aaatatcaat atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa
    40561 tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt
    40621 gcatctcata ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat
    40681 ccaagtcata gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat
    40741 tacaataata ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt
    40801 tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga
    40861 ctaatgtgtg atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat
    40921 ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc
    40981 cttcttttgg aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg
    41041 gtgttcatta caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag
    41101 gattcctatt gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa
    41161 aaacgaccga atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt
    41221 cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac
    41281 taaaaatatg agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt
    41341 caaatgggag attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct
    41401 ttgtttccca ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa
    41461 atgtccacat attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag
    41521 aatgattttg aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt
    41581 ttacaggaaa actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac
    41641 aagtctcaca ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt
    41701 ggttgaattc acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt
    41761 tcctgctaga agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca
    41821 tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg
    41881 tcgtatatgg tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc
    41941 catcaaattc tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat
    42001 aaccaaggcc ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa
    42061 ttgtaggaaa ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt
    42121 gctgaagcat gatctcagag atccagatac gataaggatc acgattatag cgccaaggca
    42181 ggtttctttt ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt
    42241 tatctgtcca tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc
    42301 tggatcatat ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt
    42361 tttttaggtt caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa
    42421 tcatattcta tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact
    42481 tcttgagaaa agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg
    42541 ctttttccgt gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg
    42601 tcttcaaatg tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc
    42661 tgcatctgct gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca
    42721 taactttccc catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt
    42781 ttatttcgat cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc
    42841 gatcgcaact gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta
    42901 ctcttcttga tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag
    42961 cgtactaata catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag
    43021 aaagggcctt tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat
    43081 atattctcta tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc
    43141 acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg
    43201 gttcctttga aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg
    43261 ttgatcatat ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa
    43321 gcaactcctc gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc
    43381 gtagtggcac catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct
    43441 tatcaagctc aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa
    43501 ccagcaggct ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat
    43561 tatagaaaca aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct
    43621 ggtttatacg aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa
    43681 cctcgaacac aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc
    43741 atctatgatg aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt
    43801 aaaactgggg agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac
    43861 gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc
    43921 caggcacaaa aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa
    43981 gccattgaag aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa
    44041 ctcaatcctc agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag
    44101 cctaatgaaa cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca
    44161 aaacaagcca aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa
    44221 aaaaatgcgg aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa
    44281 gttctgccta aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca
    44341 cgtacgggtt tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt
    44401 gtttatatct cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag
    44461 ttcaagattg aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt
    44521 gtcgtgaaga tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg
    44581 tatcaagtaa taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact
    44641 tgtgaccgaa gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt
    44701 tttgtcacag ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca
    44761 tttgtcgtat cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc
    44821 ggaagtgtga tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt
    44881 gtgatcaatt caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta
    44941 atgtagtaat cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat
    45001 gttcctggat taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca
    45061 aagccgctga ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg
    45121 atactttgcg gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat
    45181 gttcctaggt atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg
    45241 ctaaaaccga acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc
    45301 atgtagaaac ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa
    45361 cattatgctt ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt
    45421 ataacagaag tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct
    45481 ttttcaaaaa catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa
    45541 tttttttctt acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa
    45601 tattatagaa aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg
    45661 ttataggaat tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat
    45721 attactattc tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt
    45781 ggaccttact gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca
    45841 aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat
    45901 ggtctgctct attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt
    45961 tagttatttt cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctggcaatgt
    46021 ggctcatgat tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt
    46081 attttttaaa ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc
    46141 acctaagtta gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt
    46201 tctagtaccc acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt
    46261 cctgtccagc gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg
    46321 tatcttctgt ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg
    46381 aataggaaga ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc
    46441 catcttccga atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg
    46501 tatcgcttgt ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat
    46561 tttttcctaa aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt
    46621 cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc
    46681 aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta
    46741 cgccatctaa acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt
    46801 gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt
    46861 ctccattcaa tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt
    46921 tattattcac tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga
    46981 tcgttacata atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt
    47041 cgacaccacc aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat
    47101 tcgtataaat atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg
    47161 ttactaccat catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta
    47221 aacctaatag caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt
    47281 tggaggatat tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca
    47341 taaatctgat tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg
    47401 ttaatagaac tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat
    47461 tgtctcatat aactgttcaa tcagcaaatt cactttttaa tcataagaaa actttaaaaa
    47521 acatttttgt gtaacgatta ttatttttta aaatttctca aaattcttaa ctatcatacc
    47581 ataccgtgaa aaagatcgaa agaccgaata cacgcagttc gcaatcgttt tatctttttt
    47641 tatgtatttt ttccagaaaa agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa
    47701 atgatcgcac atacctttcc catcttttta acagtctttg cctaccgttc agattatcat
    47761 tcgatttcac gacaatttct tcctatgact ggtccttttg ttaaacccat cttctattcg
    47821 ttttatttcc agctcactgc atggctctta aaatgactcg aactagtgat tgcattacca
    47881 tcatctttta atggatagat atgctcctct tcaaagtaaa acgattttct tttaaagtaa
    47941 tagaattgac atggtaatga gtatgtatac tagtggtttc acaaatggtc ttttattcaa
    48001 catccaaaaa ctgaaatttc tttaaaatcc attactaacg aagaaaatgt tccttgcttg
    48061 ataattagtt aggacatgtc aagtgaacaa atgcatttac ttagccaaaa aatctaaaaa
    48121 cttcataaat agacaaaaag acacgactct ctaagaagtc gtgtctttat tcaacaaatt
    48181 attataattt agtcacttca gcagcttgag gtccgcgtgc accttcaaca atcgtgaatt
    48241 ctacttcttg accttcttct aggctcttga agccgtctcc ttgaattgct gagaaatgaa
    48301 caaatacgtc atctccgccg tctactgtaa taaacccaaa tcctttttca ttgttgaacc
    48361 atttaaccgt tccgtgttcc ataaatgtat atcctccaaa aaagttcttg caagtaacct
    48421 tgcaagaaca ttatacccaa tttagaaaac gctagcaagc tttcgctctt ttattttcca
    48481 aattattttt aagactgata tgacggagga aatggttatt ttgctgcttc gaaacgtttg
    48541 tttacttcat cccaattaat aacattccag aatgctgcaa tataatctgg tcggacattt
    48601 ttatatttca aataataagc atgttcccaa acgtccaaac ctagaattgg tgtctttcct
    48661 tccatcaatg gagaatcttg atttgcagta gaggtaatag ctaattttcc attttccatt
    48721 acaagccaag cccatccaga accaaatcgt ccagcggctg cttttttgaa ttcttcttta
    48781 aaagaagaaa aatcaccaaa agcttcatta atcgcttctt ttatttctcc tgtaggttcg
    48841 ccaccagcat ttggtgccat gatttcccag aaaaatgaat ggttagcatg tccgccacca
    48901 ttgttacgta cagctgtctt gatatctgtt ggaatagcgt ccatatcaga taataattct
    48961 tctattgttt tttcgcctaa ttctgggtat ttctcaattg ctgaatttaa attcgtcaca
    49021 taagtattgt gatgcttatc atgatgcaga tgcatcgttt cttcgtcaat ataaggttcc
    49081 aatgcgtcat atgcataagg tagatctggt aatgtgtaag tcataaaaaa attcctcctc
    49141 aatgttttct taataatgtc tacattacaa agagtaacaa agagaaggaa attcttcaat
    49201 tgttatgcct agtcgtcttt ttcactttga cctacttctt cttcaaaaac ctcagtttct
    49261 ttaaaaataa acaacttgct gaaaatatag ttcgcaagga taacaataat ttgagtgatg
    49321 attttcgcca catactcatt gctgttcata acattgatca acaataccat tgcccccatg
    49381 tccaatccgt aagaaaggat acggtagaag aagaacttac cgcattcaat aagtgcatgt
    49441 gtaaaattcg gtgattttga atggaatacc caaagtttgt tcgttacaaa agcaaacaat
    49501 acagaacaaa accacgaaat agaattggat aaggccatac ttaaatcaaa atattggcgt
    49561 gcgagagcaa aactaacaaa atttacaatc gtagccaaac caccaaaaaa gagatagact
    49621 agtatctccc aatatccttt tgcttctaaa tatcctttta actgtcgata ctgcttcatg
    49681 agccaaccta ccttattaaa aattcttatt tatcatactc tatatttcaa taactttcat
    49741 ctttatttaa aaaaattaat tttttttgat cctttgcctt tgtctctgct tggatcgtca
    49801 catgtgtaat accatatttt aattttaata aagaaattat acgaggatat atcttttcaa
    49861 tctcgcttag tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc
    49921 tatactgcca aatatggaca tgatgaacat cctttacccc atcgattgct tcaatttctt
    49981 gttccatttt ttcatagtcg atagcaggtg cactttgtat caaaatagcg gcagcttttt
    50041 tgatcaccat tatcgcttct atcaatatat atatcgaaat gatcagtgta ataactggat
    50101 cgatccagta gatatcgaaa aaacggatca aaattgctcc gataatcaca cttaccgaag
    50161 acagtgtatc acttaacaaa tgcaaataac tagattttat attcaaactt tcatctgctt
    50221 cttgtcgtaa taataatact gagaacaaat tcgcgagcaa tccaataact gctactgtta
    50281 gcatcaaatg actatttatc ttttctggtg aagtgaaccg cttgaatgct tcaacggtta
    50341 aaaacaagga taatatgatc aatatgaatg agttgatgaa agcactcaat atttctaaac
    50401 gctggtagcc atacgtacgt tttttatttg cttcttttcg ggccattttt tgtgccccgt
    50461 aagaaaaaat aattgccaat gtatcactaa ggttatggaa agcatctgaa acaagagaca
    50521 aactccctga cataatccct ccaataaatt ctgataaagt gatcaccgca ttcaaaacca
    50581 ctactccaaa tagttttttt gatgatctct gtggctgtgt aggcactatt ccttcctcca
    50641 ttttatttat attggatagt ctttagacaa gaataacatt tttttgatat ttcaccaatc
    50701 attcagctgt ttccttaaag cacttgtatt tcacttgata cttcgctaga attgcaagtg
    50761 atgacactac tagatgagga gtacttatga caacaaaaca attaattatc agttccttta
    50821 tacgattcaa agacctaaag gaggcgagaa atgtcccatt ttggaaagcc attgtctatc
    50881 ttttagtttt gagtataatt atggctttgc caatctcttt tcaagttttt caagtcttaa
    50941 acaacattca attggatggg caaaagattg ctactcgcat tcctgatttt acaatccaaa
    51001 acgggcagct tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac tccattatct
    51061 tcacatttga tccagaggga aaacgaacag aacaggacat ctctactgat ttgatgggga
    51121 attttgttag tgtcgggatg ttaaaaaaca aacttatcat agctcttcct aatacaggca
    51181 cgacttctgc tttgttgaac aataaccaat tagaacttcc ctacaccaat gaatctttaa
    51241 aaaacttaac aggtaagcag ctccgttctt ttttaagtga agcatctata ccactttgga
    51301 taaaagtcct gacatttata ttttctattt atcctagttt tctgaatctg ctgatcactt
    51361 tgctatttac caacattgct gcttttctct atgcacgctt tcgactaaca aaagctactt
    51421 ttcttgattg tttaaaaaca ttgatctatt caatttcatt gcctactata attgcaacaa
    51481 tcttgatggt ctttcttcct tcttttgaca ctagcgcctt cattgctttt gcaggaatct
    51541 ttatttttgc tcaagctgtc aaaggatggg caaaaatatc gatcaattaa gaaataaaga
    51601 caactcgtta tttctacttc aatagaccct tttagaggtt gtgacatttt tttgtcacag
    51661 cccctttttt atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc
    51721 ttcttgtatg cgctacactt agagtaaaga aaggcgggaa attaactatg tcagaaagaa
    51781 tcatttatgc agatcgtcca tttcgtgatg aatttatcga agaaatccat actatagcgc
    51841 ctggttatat ctttaaaaca gaattacaac cagaggatct acaaaatgta gagatcagtt
    51901 tgggctggaa ctcagactac caagaaaaac tattagcctc ttctaccttg aaatgggtcc
    51961 aatctatctc agctggtgtt gatcagctac cccttgagac tttttcaaag aaacatattt
    52021 tactttcaaa tggtagtggc atccacagtg aatcgattac ggagcatgtc ttaggaatca
    52081 tccttgggta ttctcgtggt ctttttcaag cacaaaaggc acagagtgac atgaaatggt
    52141 taggttcatc ggtccattac caatctatcc aagggaaaaa aatactaatc attggaactg
    52201 gccatattgg aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa
    52261 tcaatacatc cggtcatcca gcagaaggaa tggaaaagac tttttcttta aatcaactaa
    52321 aggaagttct tcctgaagta gatttcgtag tcaatatctt acctctaacg gaggaaacaa
    52381 ctgggctctt tgatcagcat atgtttgaac actttgatcc aaaatctgta ttcatcaatg
    52441 ttggccgtgg tgcttctgtt aagacacaag atctggtgca ggctttaaat aaccagcaac
    52501 tttcctttgc agcattagac gtttttgagg aagagcctct tccacaagat catcctcttt
    52561 ggaaaatgga caatgtactg attacttccc acattgcagg tttgacacct gattttcaaa
    52621 agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc catgagcttc
    52681 aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata ttttcaaaag
    52741 aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt ttttaaagtg
    52801 ataggttggc tcctccaggt tgggattata atcaacaagt aaatcatatc ctttaaagaa
    52861 ccattcatct gcttcctcga taaaaaaaag tgtcccatca atttccactt tctttacagg
    52921 atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct ctgttttgcc
    52981 gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt ctgagtcaaa
    53041 ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact ttctcttttc
    53101 tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc caataaccag
    53161 tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa ccgtttgatc
    53221 tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta attcagctaa
    53281 tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat atttatctga
    53341 caactctagg taatctaaaa tattctttga cactaaatta tgcgcaatcc ttattcccat
    53401 gtctcgctcg ggatggacga cgcgatcagc tccgatcttt tcaagtactc tggcatgata
    53461 ctcattttgg gcttttgcta aaatatttgg tacgcccatt tcctttacca tcagagtaac
    53521 taagatactg gcttgaatat cttctccaat agcgacaatt acatgatcga aatttcttat
    53581 gcctaatgat cgaagtgtca tttcatcctg tgcatttgcc acaacagcat gagtggctat
    53641 attcatatat tcattgaccc gatcttcatt actatcaata gccagtactt cttgaccagc
    53701 ttcgatcagt gttcggcaga tacttccgcc aaatcgtcca agacctacaa ttgcaaaatt
    53761 ttgtttcacc gttttcctcc tttagtatct tcttttctca gtataacaaa gcctttacaa
    53821 aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt attttctcag
    53881 cttttgatta tcctctttat catcgttttt atttcaccaa accatgtttg aatgcataga
    53941 tagcggcttg tgtacggtct tccacttcta gcttagccaa tatattggaa acatgtgttt
    54001 ttactgtttt cagtgtaata aacaattcgt ccgctatttc ctgattactt tttccttcag
    54061 aaatcagcat taaaatttca ttttcccgat tcgttagttc ttcatgtaat acaggctcat
    54121 ttcgccgact cattttttcc atcatttttg ttgtcacttc tggttctaat actcgctcgc
    54181 cacgttgcgt tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc ttcaacaaat
    54241 accctgctgc acctgcttca atggcaggat aaactttttc gtcatcaata aaactggtta
    54301 cgataatgat tttagcttct ggccaatctt ttaagattgc ttttgttgat tcgataccat
    54361 ccatttcttc catgactaag tccattaaaa tgacgtccgg tcgaagtgtc atcgccttct
    54421 catagccttg tcgtccattt tcagcttctc caattacttc aatgtcttct tgaatagata
    54481 aataaaaaga gacgcctaag cgcaccattt catggtcatc tactaataaa acttttatca
    54541 tccttatcta tgcctccttc atcaaaggga ccttgatttc tacacttgta ccttgacctt
    54601 taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg atattgttca
    54661 atccataact tcccgttttc gtttcattca tattaaaccc tgttccgtcg tcaataatac
    54721 gtaacaaaag attattgtct attttgtgca aatatacctc taattcattg gctttagcat
    54781 gtcttaatgt atttgataac aattcttgaa cgattcggaa caagtgatcc tcaatggaac
    54841 tagatagttt tacatcttct acatcccatt tcagcgaaat ctggatttta ttttgcaatt
    54901 ccttcaataa ctgctcgata ccttgcttta aacttttctc ttctaaattg actggacgta
    54961 gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct gctaccattt
    55021 tcaactgctt gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc gtcaatgccg
    55081 acatcatcat cattgctgca aacaattgtt gcgaaactga atcatgcagc tcacgggcta
    55141 atcgatgtct ttctaattct aaaatttctt ctttggtttg tccatctaca tattggggac
    55201 ggctcgttac aatttgtaat tcactagata cttcaatcat tttttctttt atcttagtga
    55261 tttccttatc aatatctttg atgtataaat cgtccgatgc acttgggatc ggaagaagca
    55321 acaatttgga ttcataattg ccagaagaaa gtgcacgcag cttttcttcg attttcccgt
    55381 attttgtctt ttgaataagt gataataata gaaagacaat taatcctaca cctaaggaaa
    55441 tggctaacaa atggaagata agcggaacat ataataaacg tgcacgcagt aattctcccc
    55501 accaatgact ttgattgctg gcataaaaat aagtgaaaag tgaaaataga ataataagga
    55561 aagccgcaat ccccgaataa acagctagca tcgctctgga tatttttccc atcatattcg
    55621 tatcacctcg acatctccaa tcaaggtatt agtaataatc ttcaatctac gagggttttc
    55681 atcataatca ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt gctcttcttc
    55741 aaacaagaca tttcctacta atgtcgcatg ctctaatcgg atggctacgc ccaaaggtac
    55801 taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat cttttggcaa
    55861 aagcgtattg cccaaatcta tgatagtatc accagaaatg acggcgatgt tgatatcgtc
    55921 ccattcatag acgtcgtttc caatacgttg gttaccgaat aattgctgtc ttttccgctc
    55981 attattatgt gtttttaatt gctctgtttg gatcatcatg atctgtttct ttcgccaaaa
    56041 agcatttttg gttaaatcta tccctgaaat ctctacacct ttcagaccaa taaataaaac
    56101 ggcaaataca gccatcatcc ataaagcagg actgttgact aagctaaaaa aaatgaccaa
    56161 acttcctaat atcaattgaa aattttgaaa tttagtacga ggatgtttgc gcatagctag
    56221 atatatattg aaaataccga atatcactaa aagcaaaagt ccagtattgt ttacgatttg
    56281 ccaaacagca aataacagca gtagtgcttc aactaccaca aaaaaacgcc aagaactatt
    56341 catcgctctt tctccctcct gacacattct catttcatca tacctatttt tttaactgtt
    56401 ggctatagga ctttagatgt aagatcgttt gttgaaatct aaaatacaca aagagaaaaa
    56461 gatctagagt gtatcatcag taagatgaac tctctagatc tttcagtcag actacgcttg
    56521 ttctactttg acgattttaa ctaacatatc gccacctgga gtagcaatag ttacttcatc
    56581 atttttacgt ttgccaatca atgcttgcgc aataggagaa tcattggaaa ttttgccaga
    56641 aaacggatct gcttcagcgc ttccgacaat cgtgtactct tcttcttctc catctggtag
    56701 ttcgatgaat gtaactgttt taccaattga tacctcatct gcatctacac cgtcgttatc
    56761 gatgatttga gcaaaacgaa tcatattttc aattgttgtg attcttcctt caacaaatgc
    56821 ttgttcatct ttcgctgatt catattcaga gttttcggaa agatctccga aaccgcgggc
    56881 aattttgatt cgttcaatga tttctccgcg tttgactgtt tttagttctt ctaattcttt
    56941 ttctaatttt tctttacctt ctagagtcat tggataaact ttctctacca tgtgtaacgt
    57001 ctcctttttt cgataaaata ccgcaaaagg taatcttttg aggaatatag tccaaaaaag
    57061 attaccacgc ttttcttcaa atgtaaatgt ttttctaaaa atgatcaatg tctagagcct
    57121 gtttttctaa gaattattta catatttttc cactaattcc atatgttctt cataagtttt
    57181 agcgaaataa acatttccag tgctcgtatc tgctacgaaa taataatagt cattttctgc
    57241 tggttctaat actgccttga ttgcctcttc gcttggattg tcaaaaggtc caggtccata
    57301 gccggtattg gtatacaaat tatatggtga atctacagct gtatcttcat acgtcactaa
    57361 ttctttgtgt tcacctaatg cgtataaaac agagatatct gactgaagtg gcatatttgc
    57421 tttgattcgg ttaaagaaga cttgtgcaat gtttttacga tcattttctt ttacgccttc
    57481 tttttcaact agagaagcta gagttaaaac ttcttgtaca gtcaaatttt tctgcttgat
    57541 agctgaataa tagttttgca tgaccgtatt cgtcttgttc accatttcaa tcactagatc
    57601 cttaagcgaa gtactcgtgt aataatcata tgtggctggg aataagtaac cttccaaacg
    57661 gtattttgtg ttctcagctt tagatgcact ttccaacagt tctgggaatt tttggtgcaa
    57721 ctcgttaaag aaagtctcat cattcactaa atctaaaaat tctttcttgt ctttaccagt
    57781 taccttcgct acccgttcag caatctgatc gatatcgtac ccttctggaa tggcaatttt
    57841 cccatctgca acttttgttg gctcagaagt accgccttct tgcaactgtt tgccaatctc
    57901 gtcaagagtc atattaggcg ctaactgata atagccagct tgaaagccag ttaaattttt
    57961 aaattttgta taataattga aaacaatccc gcttttaata atattgtcct tctccaaaat
    58021 ctcaccaatc tgcttgttag aagagccact cgggatctcg acttggacta attggtcatc
    58081 tgatttatct aaaggcttca acccgctatc tacataacga tacacggtaa atccaagaaa
    58141 tccgccaata atcaataatg taagagcaat cactaaaatg atcttcctca caatcttatc
    58201 ttctttttta cgcagatttc tccgcttgtc gattatatct gctgtagctg ccttactggc
    58261 ttctttttta tctttacttg ggtgttgttt gtttcctgac aaaacaactc ctccttacta
    58321 tcaagttcgt aa
//
LOCUS       DAYYKC010000006        57733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000006 DAYYKC010000000
VERSION     DAYYKC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.006"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1653
                     /locus_tag="ACHBBW_000387"
     CDS             136..1653
                     /locus_tag="ACHBBW_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9152100.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1625..2914
                     /locus_tag="ACHBBW_000388"
     CDS             1625..2914
                     /locus_tag="ACHBBW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9152101.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3089..3985
                     /locus_tag="ACHBBW_000389"
     CDS             3089..3985
                     /locus_tag="ACHBBW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9152102.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3999..4862
                     /locus_tag="ACHBBW_000390"
     CDS             3999..4862
                     /locus_tag="ACHBBW_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9152103.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4868..5431
                     /locus_tag="ACHBBW_000391"
     CDS             4868..5431
                     /locus_tag="ACHBBW_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9152104.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5453..6964
                     /locus_tag="ACHBBW_000392"
     CDS             5453..6964
                     /locus_tag="ACHBBW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9152105.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6976..7935
                     /locus_tag="ACHBBW_000393"
     CDS             6976..7935
                     /locus_tag="ACHBBW_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9152106.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            8021..9100
                     /locus_tag="ACHBBW_000394"
     CDS             8021..9100
                     /locus_tag="ACHBBW_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9152107.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9352..10752)
                     /locus_tag="ACHBBW_000395"
     CDS             complement(9352..10752)
                     /locus_tag="ACHBBW_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9152108.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10970..12631
                     /locus_tag="ACHBBW_000396"
     CDS             10970..12631
                     /locus_tag="ACHBBW_000396"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9152109.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12588..13286
                     /locus_tag="ACHBBW_000397"
     CDS             12588..13286
                     /locus_tag="ACHBBW_000397"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9152110.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13302..14726
                     /gene="araA"
                     /locus_tag="ACHBBW_000398"
     CDS             13302..14726
                     /gene="araA"
                     /locus_tag="ACHBBW_000398"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9152111.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            15033..16193
                     /locus_tag="ACHBBW_000399"
     CDS             15033..16193
                     /locus_tag="ACHBBW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9152112.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(16170..16310)
                     /locus_tag="ACHBBW_000400"
     CDS             complement(16170..16310)
                     /locus_tag="ACHBBW_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9152113.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16311..18170
                     /locus_tag="ACHBBW_000401"
     CDS             16311..18170
                     /locus_tag="ACHBBW_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9152114.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18280..18714
                     /locus_tag="ACHBBW_000402"
     CDS             18280..18714
                     /locus_tag="ACHBBW_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152115.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18973..19134)
                     /locus_tag="ACHBBW_000403"
     CDS             complement(18973..19134)
                     /locus_tag="ACHBBW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152116.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19153..20175)
                     /locus_tag="ACHBBW_000404"
     CDS             complement(19153..20175)
                     /locus_tag="ACHBBW_000404"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9152117.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20303..20656
                     /locus_tag="ACHBBW_000405"
     CDS             20303..20656
                     /locus_tag="ACHBBW_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9152118.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20668..21834
                     /locus_tag="ACHBBW_000406"
     CDS             20668..21834
                     /locus_tag="ACHBBW_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9152119.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21926..22546)
                     /locus_tag="ACHBBW_000407"
     CDS             complement(21926..22546)
                     /locus_tag="ACHBBW_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9152120.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22738..23034)
                     /locus_tag="ACHBBW_000408"
     CDS             complement(22738..23034)
                     /locus_tag="ACHBBW_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9152121.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23238..23888
                     /locus_tag="ACHBBW_000409"
     CDS             23238..23888
                     /locus_tag="ACHBBW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9152122.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23904..25631
                     /locus_tag="ACHBBW_000410"
     CDS             23904..25631
                     /locus_tag="ACHBBW_000410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152123.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25628..27520
                     /locus_tag="ACHBBW_000411"
     CDS             25628..27520
                     /locus_tag="ACHBBW_000411"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152124.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28224..29093
                     /locus_tag="ACHBBW_000412"
     CDS             28224..29093
                     /locus_tag="ACHBBW_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9152125.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29302..30327
                     /locus_tag="ACHBBW_000413"
     CDS             29302..30327
                     /locus_tag="ACHBBW_000413"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9152126.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30366..31316
                     /gene="manA"
                     /locus_tag="ACHBBW_000414"
     CDS             30366..31316
                     /gene="manA"
                     /locus_tag="ACHBBW_000414"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9152127.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31410..33113
                     /locus_tag="ACHBBW_000415"
     CDS             31410..33113
                     /locus_tag="ACHBBW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9152128.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33115..35148
                     /locus_tag="ACHBBW_000416"
     CDS             33115..35148
                     /locus_tag="ACHBBW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9152129.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35159..35599
                     /locus_tag="ACHBBW_000417"
     CDS             35159..35599
                     /locus_tag="ACHBBW_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9152130.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35799..36926)
                     /locus_tag="ACHBBW_000418"
     CDS             complement(35799..36926)
                     /locus_tag="ACHBBW_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152131.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36929..37606)
                     /locus_tag="ACHBBW_000419"
     CDS             complement(36929..37606)
                     /locus_tag="ACHBBW_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9152132.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37870..39636
                     /locus_tag="ACHBBW_000420"
     CDS             37870..39636
                     /locus_tag="ACHBBW_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9152133.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39651..40088
                     /locus_tag="ACHBBW_000421"
     CDS             39651..40088
                     /locus_tag="ACHBBW_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9152134.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40101..41252
                     /locus_tag="ACHBBW_000422"
     CDS             40101..41252
                     /locus_tag="ACHBBW_000422"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9152135.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41314..42012)
                     /locus_tag="ACHBBW_000423"
     CDS             complement(41314..42012)
                     /locus_tag="ACHBBW_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9152136.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(42005..43357)
                     /locus_tag="ACHBBW_000424"
     CDS             complement(42005..43357)
                     /locus_tag="ACHBBW_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9152137.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43580..44980)
                     /locus_tag="ACHBBW_000425"
     CDS             complement(43580..44980)
                     /locus_tag="ACHBBW_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9152138.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45141..45878
                     /locus_tag="ACHBBW_000426"
     CDS             45141..45878
                     /locus_tag="ACHBBW_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9152139.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            46003..46482
                     /locus_tag="ACHBBW_000427"
     CDS             46003..46482
                     /locus_tag="ACHBBW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9152140.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46479..47246
                     /locus_tag="ACHBBW_000428"
     CDS             46479..47246
                     /locus_tag="ACHBBW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9152141.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47476..48045
                     /gene="lepB"
                     /locus_tag="ACHBBW_000429"
     CDS             47476..48045
                     /gene="lepB"
                     /locus_tag="ACHBBW_000429"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9152142.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48149..48751)
                     /locus_tag="ACHBBW_000430"
     CDS             complement(48149..48751)
                     /locus_tag="ACHBBW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9152143.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48857..49087
                     /locus_tag="ACHBBW_000431"
     CDS             48857..49087
                     /locus_tag="ACHBBW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152144.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            49078..49326
                     /locus_tag="ACHBBW_000432"
     CDS             49078..49326
                     /locus_tag="ACHBBW_000432"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152145.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            49432..50016
                     /locus_tag="ACHBBW_000433"
     CDS             49432..50016
                     /locus_tag="ACHBBW_000433"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9152146.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            50019..51092
                     /gene="prfA"
                     /locus_tag="ACHBBW_000434"
     CDS             50019..51092
                     /gene="prfA"
                     /locus_tag="ACHBBW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9152147.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51085..51924
                     /gene="prmC"
                     /locus_tag="ACHBBW_000435"
     CDS             51085..51924
                     /gene="prmC"
                     /locus_tag="ACHBBW_000435"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9152148.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51917..52096
                     /locus_tag="ACHBBW_000436"
     CDS             51917..52096
                     /locus_tag="ACHBBW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152149.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52118..53143
                     /locus_tag="ACHBBW_000437"
     CDS             52118..53143
                     /locus_tag="ACHBBW_000437"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9152150.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53334..54578
                     /gene="glyA"
                     /locus_tag="ACHBBW_000438"
     CDS             53334..54578
                     /gene="glyA"
                     /locus_tag="ACHBBW_000438"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9152151.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54731..55678
                     /locus_tag="ACHBBW_000439"
     CDS             54731..55678
                     /locus_tag="ACHBBW_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9152152.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55812..56441
                     /gene="upp"
                     /locus_tag="ACHBBW_000440"
     CDS             55812..56441
                     /gene="upp"
                     /locus_tag="ACHBBW_000440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9152153.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56580..56822)
                     /locus_tag="ACHBBW_000441"
     CDS             complement(56580..56822)
                     /locus_tag="ACHBBW_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152154.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56943..57015
                     /locus_tag="ACHBBW_000442"
     tRNA            56943..57015
                     /locus_tag="ACHBBW_000442"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56976..56978,aa:Thr,seq:cgt)
     gene            complement(57134..>57733)
                     /locus_tag="ACHBBW_000443"
     CDS             complement(57134..>57733)
                     /locus_tag="ACHBBW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HFM9152155.1"
                     /translation="GILGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDT
                     KKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILP
                     IMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQ
                     GVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        19197 a        10103 c        11942 g        16491 t
ORIGIN      
        1 aaattatttg ttttaatcat tattaatatt ttggttaatt aaaatcgata aaaaataagt
       61 aagtttaaca tttatattat tgacaacgct ttcaacgagg tgtattattc atgtataaat
      121 taaaggaggt cagttatgga agccacaatg ttgattagta gtgtcaagcg tgtaggcaaa
      181 gttgatccgc ggttgtacag ttcatttatt gaacatatgg gacgagcagt atatgaaggg
      241 atttatcagc cagatcatcc atcagctaat aaaagtggat ttcgccaaga cgtcattcac
      301 ttggtaaaag aattgaacat tcccatgatt agatatcctg gtggaaattt tgtgtcagga
      361 tttcgttggg aagacagtat tgggcccata gcaaatagac ctagaaggtt ggatttagct
      421 tggcggacaa ttgaaacgaa tgaagttggg attcatgaat tttataactg gtgtcagcat
      481 gtgggggccg aaatcaatat ggctgtcaat ctgggtactc gtggagtaga tgcagctcgt
      541 aatttagtcg aatattgtaa ttttcctaaa ggaacttatt ggagtgattt aaggcgttcg
      601 aatggtcaag agagcccttt tggaattaag acatggtgtt tagggaatga aatggacggt
      661 ccttggcaga ttggacataa aacagcggaa gaatatggac ggttagcaga ggaaacggct
      721 aaggcgatga aactcgtaga tgattcaatt gaggtggttt tatgcggaag ttcgaatcgg
      781 caaatgccta catttggtga ttgggagtta acagttcttg atcaagcgta cgatcaaata
      841 gattatttgt ctctgcatca atattatggc aaccaaaaaa atgatctttc tcattatctt
      901 gcacgttcct tagacatgga tcggttcatt tctgaagtga cagcgatgtg cgatacagtc
      961 aaggcgaaga aacatagtaa gaagcagatc aacctgtcct ttgatgaatg gaatatttgg
     1021 tatcattcag cggaacaaga tgagcaagca gcgccttggc agattgctcc tcctttacta
     1081 gaagataact ataattttga agatgcatta ctattaggct gtctattaat cactttattg
     1141 aaacatgcag atagagtaaa gattgcttgt cttgctcaat tggtaaatgt tattgcacca
     1201 atcaggacag aaacaaacgg agaagcttgg agacagacga tctattatcc atttatgcaa
     1261 gctgctgtgt atggacaagg agaggtgtta agcccacaaa ttcgttcagc aaactatgcg
     1321 acggaagatt ttgaagaagt gccgtattta gaaagtatag ccgtaataaa tgacaaagaa
     1381 cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga atctaactgt tcacttgtct
     1441 gaattatcat tagagggagt gattgatttt tctgaaatga gtggatttga tacaaaagca
     1501 actaatcttc ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt aaaagctgaa
     1561 gatgggaaaa tcaattgttt attgaaaccg ctatcttgga atgtggtcag atgtcgttta
     1621 tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct ctttgttggt
     1681 agctgcaaca ggtattttag gagcttgcgg tggcggaagt tcagcagaat ctgattctga
     1741 taaagaagtg actttgactt tttggaatgg gtttactgca tctgatggtg aaatattgca
     1801 agaaatcgtt aatgaattta ataaaacaaa tgataaaaac atcaaaattg aaatggatat
     1861 catgacttgg gccaatttga atgaaaaact tcctacagcg atttcttcta aaaaagcacc
     1921 tgactttatt gcattaaatt atccagattt tgcacagtat gtagaaaacg gagcggtaca
     1981 accattagac gatttctggg aatatgatgg agtagataaa tctgatttta aagaaacggc
     2041 tattgaatta ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg tacaaggcat
     2101 gtatatgtat tggaataatg atttgtttaa acaagctgga ttagatactg aacaaccacc
     2161 tactacttgg gaagaattga cagaaatggc accaaaatta actgatagta gtaaaaatgt
     2221 atcaggtttt gtcttcaata aggatggaac tgcaccgtta tataactgga tgttagcaaa
     2281 tggcggtaaa ttagtgaacg atgattatac taagtctgaa tttgcgtctc ctgagaacct
     2341 taaaacgtta aaagcgatcc aaaaaatgat ccatgtagac aagactggtc cagaatcgat
     2401 ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa ctagccattg aaatcaatgg
     2461 gccatggtta aataacggat tgaaagcaaa cgagattgat tataatgtga ctaccttacc
     2521 acaagggagt gattcagaca aaaaaacagc tattttagat ggcgtaggat atgccattcc
     2581 ggcaagtaca gataataata aaaaagaagc tgtctatgag tttttgaaat attggaatac
     2641 aactgaaatt ggtaaaaaat ggagtatcga aaatggtttt cctgcctact tggattcagt
     2701 agcaaaagat gaagaagtaa aaaatgatcc tattgtatct gaattatcga aacaaatgga
     2761 ctatgcagag ccatatttac caggttttac ccaaatctct gcgatcaata acgatatcat
     2821 caatccactg attgaaaaat tattagcagg agaagatcct gagacactca tgaacgatgc
     2881 agatgatgcc atcaatcaat tactgcaaaa atagtacaga actttataca ggatagactg
     2941 ttggcaagat acagatatct gaatgatcga cctagtattt gcagataggt gttattgcaa
     3001 aaatcacagc gattgttcgg atatcttatt cagattcgtt taaaccaaga ttgtatattt
     3061 tatcaaaatg aaagatgagg tggactatat gaacggattc accaaatatt ggaatcgtcc
     3121 tgatcgtgct gcgtatttgt ttttaactcc atctttttta gttttattat tatttactgt
     3181 cataccgctt ttaggaacat ttgctattag ttttacagat ttgaatattt tctttacatc
     3241 aagaaatttt gtaagttttg aaaatttcac acgtatattt agtgatgaac gagcagttaa
     3301 ttcgttgatt cattctttat atttcaccgt actcgaaacg ccaacacaaa ttatcgttgg
     3361 tttattagca gcagtagcat tatctaaaaa taccagattc aatcgttttt gtcgttcgac
     3421 attttacatt ccagttattt gttcattgac gtcaattgcg attatttttt ctatgttgct
     3481 tgatccgaat gtaggggcga ttccttactt gttttcacaa gtcggcttgc cgatccctca
     3541 attttttcgc gatccaaccc ttgctatgcc gacggtagcc atcatgactg tttggaagaa
     3601 ttttggtgtt actatgacga ttctcttgac tgctattcag ggaatctctc cctctttgta
     3661 tgaatctgct caaatggatg gtgcaacaaa tcgacagcag ttttttaaca ttacgctacc
     3721 tcagatcgtt ccctccttag gtttttgtat tttaacgaat ctgattggtt cgatgcaagt
     3781 gtttgaccaa gtatatgttg caaccgctgg tggcccacaa tttaagacag aaacagctgt
     3841 tcaatatatt tacagtcgag gatttacagc accttatgaa ttgggctatg catcagcact
     3901 gtcttcggtt ttgtttatca ttgttgcaac tatagctatc tccactaatg tgtacatgtc
     3961 acggaaagaa aaacaaatga aataggaggg aaacctgtat ggcaccagaa agctcgatta
     4021 cagttactgt gaatagtcga aaagcagtta gtggtaaaaa aatcattcga acgattagta
     4081 tgctagttat attgttggtt attgcgtttc cttttttgtg gctgattatt tcttctttta
     4141 aacacgaaaa agacattatc tcatttccgc ctagaatatt tgcagatagc tataccctag
     4201 ataactatat taaggtttgg acaacgatcc ctttattaga ttacatcaaa aatacagtga
     4261 tttttgctgg agggacagtg atcacttctg tattctttga ttctcttgcg gggtatgcat
     4321 ttgctcgtat gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg ctgacaatga
     4381 tgatcccatt ccaagtcttc atgattcctc tatttattga agtcaatttg cttggcatgc
     4441 ttgatactta tgcaggtttg atcattcctc gtatgacaac tgcatttggt atcttcatga
     4501 tgcgttcctt ttttattact ttaccagatt ctttggaaga ggcagcacgc attgatggat
     4561 taagtgagtt taatattttt ttgaaaatca tgttgccatt gagcaaacct actcttttat
     4621 cattagggat tttcacgctg atgaatagtt ggaatgactt gctttatcca ttgatattga
     4681 caagtagttc aaaaatgaga actttaccag cagggttagc tttatttaca gggcagaaca
     4741 tttcttttta tggacctgta atggctggaa cggttatttc aatgctgcca ttactagtgg
     4801 tttatatttt tgcacagaaa tattttgttc aaggaacagc tatgtctgga atgaaagagt
     4861 gaggaagatg aaaatagttg agactaaact ttatggacag ttgattgtgg tttctgatta
     4921 catcttatta ggtattctct gggtaatcgt gtgtttgcct gttattacta tcgtgcctgc
     4981 tacatgtgct gttttttatg taatggcgca atggaaaggt gagggatcag gaaatatcat
     5041 ttattttttc tttcaaggaa taaagcaaca ttttggtaag aacttgttcc ttagtctgtt
     5101 aacgatcatt atttattttc ttgttaatca attattagaa gaacagacaa tcgtactttc
     5161 aattagtgga tatgtagttt tacttattta ttccatgttt cttttatcct ggatatatgt
     5221 gatttctgaa aacaaagata tcacattttt acaaattttt gaacaaagtg catggaaagt
     5281 attttttgat tttttgagaa atattctttg ttgcgtattg ttattaggat ttgtactgct
     5341 catatttctt ttccctcctt ttatttttat ctttgctggt ggcgtatgga aacttgtatc
     5401 tattatcttg actataggaa atgaaaggta gggaaagctg ttggacgaag aagtgaaagt
     5461 aaaaatcaga gaggatttta cggtaggaga gatcagttct cggctgtttg gctcatttgt
     5521 tgagcatatg ggaagtgtcg tctatacagg gatttatgaa ccgggccatg aaacagctga
     5581 tggtgacggg tttagacaag atgtcctctc ttatgtaaag gaattagaat taggtgtcat
     5641 tcgctatcct ggaggaaact ttacatctgg ttatgattgg gtggatactg taggtaggaa
     5701 agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg
     5761 actccacgaa ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa
     5821 tttaggaaca aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa
     5881 aggaaccttc tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa
     5941 attatggtgt ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta
     6001 tgaatatggt aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat
     6061 tgaaacaatc ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg
     6121 tatcgcatta gaagaagctt atgagaatgt agattattta gctttgcata actatattga
     6181 taagtatgat gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac
     6241 ataccttgca aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga
     6301 ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa
     6361 tgtacatgcc caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg
     6421 gtgttacttt actatggaag atacattatt atttggttca ttagggctgg ctattttacg
     6481 acacgcagat cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat
     6541 actgacagat gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt
     6601 atcgaaatat gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg
     6661 tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct
     6721 gactattttc ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt
     6781 caatttgcta gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa
     6841 tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca
     6901 tgacgaatta aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat
     6961 gtgaggaaat ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca
     7021 gatccttggg tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc
     7081 taccaagtga ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc
     7141 ctgattgtat ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa
     7201 gtccattata tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata
     7261 cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg
     7321 aatacagatt ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac
     7381 ggaacaactt tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa
     7441 attactggga attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg
     7501 aagcagatcc tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat
     7561 gaaggtccag cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca
     7621 acagatgaaa actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta
     7681 aaagaatcat ggcataaatt atcggagcca gtattcgttt cttctaaaaa aaataagcaa
     7741 tttggtcctg gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc
     7801 tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac
     7861 gcgaacgcgc aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt
     7921 ccgtatgcct tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg
     7981 ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat
     8041 tgcagaagag ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc
     8101 ctcagaattc aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat
     8161 cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga
     8221 tgacagctat aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac
     8281 cacatatctc tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg
     8341 agaatctggg tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga
     8401 gtgtttgaag aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag
     8461 taatcagtat aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat
     8521 ggtgatgatc aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt
     8581 gaaagtcggt tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt
     8641 catcacaaaa atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg
     8701 tgaacagtat aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg
     8761 agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat
     8821 catttgctat aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta
     8881 tcatattcca gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg
     8941 gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg
     9001 gtggatcgtc acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt
     9061 actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct
     9121 cttattgctc ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg
     9181 ctgaacgttt ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag
     9241 tcacaatatt catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac
     9301 taagcggttc cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg
     9361 atggatagaa atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa
     9421 agatttccca cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat
     9481 gatcccaaaa atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag
     9541 caagccaact aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc
     9601 gcggacagct agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca
     9661 agaataaaat gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc
     9721 atccagtaca gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc
     9781 tattttcaat aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc
     9841 agcaactaaa atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc
     9901 agtcgctttt tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg
     9961 ctggaatact gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa
    10021 agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa
    10081 ttcctgttca gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa
    10141 gcggccagat tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa
    10201 aatcagcgcg gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa
    10261 cccttttagc aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg
    10321 attgatccct gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg
    10381 aactagggca gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa
    10441 ataagaacca ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc
    10501 agaaagaaga atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc
    10561 gccaaggatc gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc
    10621 tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata
    10681 tccaaataga atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt
    10741 ttcttttttc ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt
    10801 gtaagcaatt tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct
    10861 tgtaaataac tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact
    10921 aaaaagacgg tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt
    10981 aaatgcgcaa agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg
    11041 atcaactagg atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag
    11101 ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg
    11161 gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga
    11221 gaatcttacg acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt
    11281 tgatagcaag gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga
    11341 agcagaacga atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc
    11401 tcatttgtat caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac
    11461 aacgcttgca ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga
    11521 tgcatcaggc atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa
    11581 tatttttggt cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc
    11641 taaagttcta gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat
    11701 cgaccctacg ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg
    11761 aaccggtatg gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac
    11821 ttccgccttc gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga
    11881 catggtcact acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc
    11941 ggatatcaat gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat
    12001 aagcagagat aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg
    12061 cgggggatta ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg
    12121 aaggccatta tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat
    12181 ccatttagcc agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa
    12241 tgtccaaatt gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca
    12301 gcggattcta gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg
    12361 tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt
    12421 agaaaacttc ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc
    12481 agcagaagaa atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat
    12541 tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac
    12601 tgaaagaaga agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata
    12661 catggggaaa tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg
    12721 gagtcagtta tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata
    12781 tcattgaagg taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt
    12841 catttcctca aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac
    12901 aagcaggatt ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg
    12961 taccttgtgc tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag
    13021 gaaaagttat catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg
    13081 tacttttaca cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga
    13141 acgcagtcgt actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat
    13201 tttcagaaga gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa
    13261 atgcgtatta cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa
    13321 aagaattttg gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag
    13381 taaagaaaca tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc
    13441 caatccgcct tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg
    13501 tcaattatcg agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa
    13561 aaatgtggat ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat
    13621 ataacgaaag cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg
    13681 ctcatggtga ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg
    13741 tcgttggcta ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg
    13801 cagtagccta taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa
    13861 acgtgggtgt cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg
    13921 attattttgg tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg
    13981 atcaattatt tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa
    14041 agacatggga ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct
    14101 tagaagaagg aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac
    14161 aactacctgg actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag
    14221 gcgactggaa aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata
    14281 caggattcat ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat
    14341 cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc
    14401 ctttatcaat gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag
    14461 aaggggtagt cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag
    14521 tagaagcctt tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga
    14581 aaactaaacc gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc
    14641 ataccgttgt ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg
    14701 agttggaaac agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga
    14761 aataagaaga gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca
    14821 atgtcagcta acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa
    14881 aataaggctt atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct
    14941 ctaactgctc tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat
    15001 ttcgagatac ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta
    15061 ttccagaaca agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc
    15121 cagccgaagg aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt
    15181 tgctagcaat ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg
    15241 aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg
    15301 ttttgtgctg tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa
    15361 cactaacagg tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac
    15421 gagaattgca aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag
    15481 cgctaagttg ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt
    15541 tggattatgt tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag
    15601 tcatgctgcc gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa
    15661 tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg
    15721 ttatgcaagc attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag
    15781 agttcttaga aaatgaccgt atacttatgt tgcctcatct agggggatcg actcaggaag
    15841 ctttggcaga cagcggacgt ttggcagttg aagccttaaa agattatctt ttgttcggta
    15901 cggttagaga agcggtcaat tatccatccg cacgaatgtt tttccaagcg ccgtttcgat
    15961 tcactatatt ttatcaaaag aaaaccaata tattagcaga gatattttct ctgctgaatg
    16021 agaacgagct agcgatcgca gatatatcgc gtaaccataa gaatgagcat gtctacatat
    16081 tgattgacat agacagtact gattttgaac agctcaatca agtgaaacaa cagctagaaa
    16141 aaatttctgg agtacgaaag gtccgcttat taaagaaacc agaaaggcca taaagaaaga
    16201 gaatatttgg atttcattaa ttcttctttc cgttctttac tttcgaattt cgtacaacta
    16261 aaataagaga agcaacattc ttttattgat agaaatgaag gagcaagcat ttgaaaagat
    16321 atataagcag actttcgacc aaacagaaaa agattttaaa aggaacagca gcaatcatca
    16381 gtatcttttt tcttgttgtt tttagtaatt tatttttgca gtggtgtcaa aatgatctat
    16441 ccgtagacct tgcgttgaaa tttgcttttt cttggcatac ggaaaaattc ttcttagcct
    16501 gtctcgtctt gctgattatt cttatttttc tgattgcact agcaggatct gtccctttag
    16561 gttcccttac gtatgtcgta gccatcggtg tattaggttt tgcaaattat atgaaaatga
    16621 gttatcggca agaaccgatt tatccagacg atttgaagat gatcactgaa atcggattgc
    16681 tgaaagatat gaccggcact atgctgttta cagtgatctt agctgccgca ggaaccgtga
    16741 taggattgtt ttgctggtac atgtttcgaa gcctgaaaaa aggaaggcga ttccaattga
    16801 tccgattaac gacactgctt gtggcaattg gactgctagg gtatatcagt aatttcaaca
    16861 atcccgataa tttgttgcga aaagcatata ataaaacggc actatggata ccttacagcc
    16921 agaaaatgaa ctactataac actggtttta tcggtgggtt tttgtacaat ctgaaggtag
    16981 aaccaatgga tgaacctgaa ggatattcga aagaaaagat caaagaaatc actgaaaaat
    17041 accagaagct agccgatgaa aaaaataaaa ctgtagagga agagtcgccg aacatcgtgt
    17101 tcgtgatgag tgaaagtttc tcagatccta gtcgattaaa cggcgtagaa gtatctggtg
    17161 agccattggc agattattat gaagtagctg atcaaacata tagtggcaat atgctttctc
    17221 aaaactatgg cggtggaaca gcaaatatcg aatttgaagc attgactggt ttttctatgg
    17281 cgttatttaa tgcgcaactc accacccctt acacgatgct tgtaccaaaa atggatcagc
    17341 ttccttctat cgtttcagcg ttaaacgcac aatcatacca gacgacagcg atccatccat
    17401 acaatacttc gatgtataaa agagaggatg tttatcagac attaggattt gaccagttca
    17461 tcagcgagcg tacgatgacc tatacggata cgatagagaa taatccatat atttctgatg
    17521 agtcagctta caaagaagta atgacacttc tcaaggaaga aaaaacacct cagttcattc
    17581 atttagtcac gatgcagaca catatgccct acgatggaaa gtatgataat ctagactatc
    17641 ctgcaaaaac ttcagatgga agtgggacac ttgacttaga gaattatctg caagatattt
    17701 cctatagcag cacggcactg aagcagttca cggaagaatt gaagaatctt tctagaagga
    17761 ctttagttgt tttttggggg gatcatcttc cagggatcta ttcagatacg atccaagcaa
    17821 aaaatgacaa acaaacattg catgaaacac aatttcttat gttcgacagt aaaggagaat
    17881 tagaaaagca aacgactcac gatgcaatca ccagtccgtt ttattttgca gcgaatctaa
    17941 tggaacagac gaatcagaca acaaatggct tttatcagct gcttttatca ttagaacagg
    18001 aacttccggc gtttgaacga gaactgtact atcaaaacgg acagtggtat aaagaagcgc
    18061 aattcaatcg ctcgcaacaa gagatttacg acgaatatca attgatccaa tatgacattg
    18121 tcgcagggaa acaatatagt ttagcagatg gtttctttga gcacgaataa gaatggcgag
    18181 ctgtatagaa accattaaat tttttcaagt acttgtcatt tgaggataaa aacaagtaga
    18241 ataaataata acaacaatgg agaaagtagg aaaacgaaca tgttaccgaa tattggctat
    18301 tatgtgcagt ccatcgatca aatcgtcaag gaaacagaag caatcggaga aggaatgcat
    18361 ccgaaatatg aagtgatcag aaacgcaatt gatgaaggaa aaacaagtga actggacaaa
    18421 tcgactcttg aagagatcat cctgttattt gaagaaggaa caaaaaaata tcgggcaatg
    18481 cttatgaaag tcagtacttt gcgtccacca gcaaaagcaa tgggcattca taaaaatttt
    18541 gaaaaagctt atctttcata tgtagcaggc tgtgaagaaa tgatccaaag tatcgatctt
    18601 gaatcagggg tagatgaaga tctattcaat acatctgaag caaaacaaga ccaagcgaca
    18661 gaccaattga cagctgccat ccaaaaaatg actaatttgt tgttgaaaaa ataaaagaaa
    18721 tctttgataa caataaaaac tagagttatc taatataaat gtaatattac ctttatgata
    18781 gagtgatcaa aatcctttat gataatgaca tacctataac caacaacaac cttgtactta
    18841 ctcaaaatcg agtaaatact ccttttccgt cgagtctaac catctcggcg gttttttatt
    18901 tgtagaaaag aacatcagga tagacggaaa agtcttgtcc tgatgttctt ttcagatagt
    18961 atggaaagct gattagtttt ctacattctg tacatattgt tcaaagtagt tctgtaaatc
    19021 ggctttgagt ttctgatatt cctcttcgct atactcttta tcttcgattt tcccatcaaa
    19081 gaaaggaata tttagttctt cccgtagtct gtctaatacg tcttgctgat tcatttcatt
    19141 ctccttttct tactaacgat caaaataaac acagacacat tctggtacat agacatcttt
    19201 tttcagtaat tccagtaatc ctgtttcagg atgacggcga tataatgtca gattatcggt
    19261 gttctgattg gcacacacga caaaattatt gcttggatct agtgcgaaat cgcggggaaa
    19321 atccccttct gttgagatga tttgcttgtt ttgaatgtgg gaaccatctt caaaaactgc
    19381 gaatacagca atcgagttat gtcctcggtt agaagcataa aggaacttgc catcatttga
    19441 aatgcggata gctgcccctc cattttcacc gtcgaattct tctggcaagg tcgatatttt
    19501 ctgtaattct tcaaaagcac ctgtttcttt attataagaa agcacgcgga ctgtgctgtc
    19561 taattcacca aataaataag cgaattgtcc atttggatga aagacaagat ggcgagggcc
    19621 tgttcctggc tctgcagtga aggtagttac aagattcaat ttgccgtttt cggacacatc
    19681 ataagtatac acacgatctg taccaagatc acatacgact aatcgttggt caggagtcag
    19741 atccgtatag tggacatgcg catgatcttg gtttttatga ggacctgtcg cttcttgatg
    19801 gaatattgaa tctgctgctt tcagttcgcc atctcctaat atatggtaga catttacttc
    19861 acctttatga tagttcgccc cgtatagtaa ttgacgtgct tcatcaaccg ctacataaca
    19921 caaaggagcg ccagactctg ttaccgcatt caaaaaatga aaatccttat cgtacgcacc
    19981 ggcaccacct aatccatcca cactagtaac tgtgtaaaga ttgccagctt tactttttgc
    20041 tacataggta gggctatttt cttcaacagc taatgaaagg ttggctaatt cttctttttc
    20101 tgtatcaagg tcgatcgtat agattccttt actttcgcga cgcgtatagg ttcctaaaat
    20161 tatcttttcg atcatatgag ctgattcctc ctaattgtaa tgaaatagtt ttcgttttta
    20221 ttctatcaca taattattcc acacggctaa agaatgagaa tgtgttatac tgtttttgaa
    20281 agcaattaaa ggagagagtg caatgattca ggcaaagatt acagaaattg ggacagaagc
    20341 aataaacaag gaagaaccca tgctgatttt gttcgataaa acagcaacag ctgccttacg
    20401 aaaatattcg gtgattcaag aagtatcaaa agacgaacct ttttctttga aagcaggcgg
    20461 aacaattgct tttgatgatc aggtatatac gatcgattat gtcggtccga tggccaatgg
    20521 gaatttaaag acagtagctc acgtatcatt gatttttgat gcttgtccag aagaaggaca
    20581 aattgcaaat ggaatctatc ttcatccgca tagactgcct gctattggtt tgggaagcca
    20641 aattttttat aactaacagg aggaattatg gaaggaaaaa ataagaagaa cagaccggta
    20701 tcgtggtttt ggcgctggtt tttaaataat caagtagtca cagccttact tgttgtattg
    20761 ttaatattgc tgatcgtctt tttatttaca aaagtttcat atttgtttgc accaatctgg
    20821 caatttttag caatcgtagg tctgcctatc attttagcag gaatcttgta ttacttgatg
    20881 aatccggtag ttgattactt tgaaaagcga aaagtaccaa ggctttacag tatcattggg
    20941 ctgtttgttt tggttgttgc actaatcgca tgggggagcg tagtgatcat ccctaagatt
    21001 caggaacaaa cagtgagctt catcagtaac ttccctcaat atgtagatac gattgataat
    21061 aaattaaccg agattttgcg ggacccgctg ttcaaccaat ttcgcgaaca gttggaaaca
    21121 gcaggtgata agttcatgaa ttcggcaggc gatatgatcc aagatatatc taagagtaca
    21181 gttcagagtc ttggaagttt tgtcggcgcg gtagcgacta ttttagtggc tctgctgacg
    21241 atgccgttta tcttatttta tctattgaaa gacggtaaac aattagcacc ttattttgtc
    21301 aaatttttac cgacacgcat gcaaaaaccg acgttgaatg tgctgagtga aatgaatagt
    21361 caagtttctt cttatataag aggacaacta actgtggctt ttgctgtggc aatcatgttt
    21421 atgattggtt ttgcggtcat tggtttagag tatgctgtca cgttggggat tattgcgggt
    21481 tttttaaacc tgatccctta cttaggatca tttttagcga tgattccggc tatcttttta
    21541 gccattgtag caggtccatt catgatcatc aaagtactga tcgtttttgc agtagagcag
    21601 acaatagaag gacgcttgat ctctccatta gtactaggga atcagttagc aatccatcca
    21661 gtaacgattt tattagttct gctgacttct gggaaattgt tcggaatcat cggtgtgatc
    21721 ttggggattc ctgtttatgc agcagctaaa gtagtgatta ctcatgtttt tgaatggtac
    21781 acaacaatct cttctcttta tgaagaaaat ccgttgaaaa agcagaaaga ataacaaaaa
    21841 gagacagatg ggaaaatatc caaggcacaa atgctgcttt gaagattttt tcctctgtct
    21901 cttttgtatg catttatctc aatttttaaa atgatttacc taaagcagca gctttatcta
    21961 atgcattttg catcaactca tctgcacggt cagggtcata atcgattcct tcgatgaata
    22021 gctgatctac ttgatcgatg cctacaaagt ttaaaatacc ttttacatat tgagaggcaa
    22081 agtcttgtcc gttgtataca ccgccattag attggatgtg cagtgctttt ttacctgtca
    22141 ctaatccttc tggcccgttt tctgtatagc ggaacgtttt tccagctaca ttgacggtat
    22201 cgaaccaagc ttttaatttt gttgggatat ttagattcca taaggcgttt gcgataacga
    22261 ttttatcagc agcaaggaac tgatcagtca attcattgaa acgagcaatt ttttgttgtt
    22321 gactttctga taaggaagtg aattctgcac cagcacgtaa tgcttcccaa cctgataata
    22381 attcttcatc gatttccgga atatcatcgc gataaagttc caaaacagtc acttcatcat
    22441 ctggatgatt ttctttataa cttgttaaaa aagacgtcaa tgccttaact gtgcgcgatt
    22501 cttctgcagt taggggatga cctttgacta ctaaaacaga tgccatatgc ttaccttctc
    22561 cttttattgc tatgattgtt ataactatga ctaacaaaat cagtttactc gaaaaagaaa
    22621 aggctatcaa gtcatattga taaggttgaa gaataaataa ctaaaaaaag acagggtata
    22681 aaagaggctg tgacacgatt ttttgtcaca gcctcttttg aatctaacaa gcaatattca
    22741 agcaacactc gactgaagag cttccatccc accaatgata taaacagcac gaaaaccatt
    22801 agcagtaagt tgatcggcta atgcttctga tcgcctgcct gtatagctta gaatataata
    22861 aagtttcttt ggatctagat tttttaattg tcttggcaag atagcagcgg ggatagaaag
    22921 cgagtggctt aagtggtttt ttgtgtaatc taaggtattt cttgtatcta aaacgatgct
    22981 gtcagcatcg agaggcaatt tattgaagtc ttggacacta attgattggg ccatttatct
    23041 catcctttgt ttagtatgtg gctattttaa tgggcaactc tcattaattc aaatggaagg
    23101 attcctctta tcactttttt aatgtatcgg atgataatga aaaaaactat gcaaatgtct
    23161 tgcaaaacag gtaggaggtg ttataatgac atagcgtcat atgacaatgt gtcatcttga
    23221 aagaaaggag aatccatatg ccgacacaga catttttcca tttgcctaaa gaaaaacaaa
    23281 aaagactaat cgaggctgcg agaatcgagt tttctagagt acccttaaaa gaagcttcga
    23341 ttgctaatat cgtcaaatta gcggatatcc ctagaggcag tttctatcag tattttgaag
    23401 ataaagagga tctttatttt tattactttg agacaatgag acgtgacagc tctaaagata
    23461 tgttacaact aatgaaagaa tccgatggtg atctattcaa aggattcgaa tcctattttt
    23521 ctaaaatgat ttatgagatt ttggaaggcg agaatgcttc tttttatcgt aacttgttta
    23581 tgaacatgga ttaccgtgcc tcaagaaaag tcgctcctca tacgagtcgc caaaaaacgc
    23641 ctcacgatcc acataaagaa cacaaacaag atgcaaaaga gttgcttgaa ttgatcgatc
    23701 ggacgaagct gaaagtaaaa aatgatcatg aattggaatt actgatccaa ttagtgatga
    23761 atactgtatt tacaacaatt gcgcattcgt atcgcttatt ttcaacatct gaagattact
    23821 cagtagagca agcaatcagt gaattcaatt tgaaactttc ttggctgaaa aacggagtat
    23881 ataaatagaa agaagggaac tagatgctaa aaatcgtaaa acggatctcc ttgacttcag
    23941 caattgcagc tgcagttttc atggtgatcc aagtactggc agatttatat ttgccgacac
    24001 ttacctccaa tattattgat aaaggggtag cgcaaggaga tgtcgattat atttggcata
    24061 caggttttgt gatgatcggc ttttctttga tcagtatctt agctgctatc gcaaatacat
    24121 tttttgctac tcgtgaatca cagaaactgg gtaagcaatt gcgtacggat gtctataaaa
    24181 aatcagagag tctgacaaaa gatgcgtttg ataaatatgg aacggcctct ttgatcacaa
    24241 gaacgaccaa tgatgtgact caaatccaaa tggttacaca aatgttttta cgtatgatga
    24301 tcaatgcacc aatcacgttg attggcgcga gcatacttgc ttatcaaaaa gatcatcagt
    24361 tgacaaaaat cttcttagtt gtcatcccag taatgattat tttgatcggc ggaatcatgt
    24421 actttgctgt tccattattc aaaagtatgc aaaagaaaac tgaccgttta aacctggtat
    24481 ttcgagaagg attgacaggg gtcagagtga ttcgagcatt cgataagaca cgctttgaag
    24541 aaaatcgttt tgatttagcg aataaagatt atacaaatac ggcaatcaaa gttaatacga
    24601 tcgtcgcatt gatgatgcca atgatgacat tgatcatgag cggtacaaac gtagcaatca
    24661 cttggtttgg tggacactac atagcagata tgactttgga agtcggtaat ctgattgcat
    24721 ttatgactta tgcgatgcag atcttgatca gctttatgat gctttccgct atttttatca
    24781 tggtgcctcg tgcacaagct tcagctgacc gtattaatga agtgttggat gaaaaaatcg
    24841 gtatccatga ccctgaaaat cctaaaacag tttcttttgc tggaaaaaac gcaacattgg
    24901 ccttcaatca tgtcaattat cgatatcatg gagcagaaaa acttgcattg gaagatattg
    24961 atttccaagc caagtcgggc gagatcgtcg caatcatagg ggggactggt tcaggtaaaa
    25021 cgaccttagt caatttgatc cctcgattat atgacatcga atctggttcg atccaaataa
    25081 atggaacgga cattagtgac atgactcaat ataatttgcg agaattaatg ggctttgttc
    25141 ctcaaaaagc agttctgttc tctggaacga tccgagacaa tatgcagtat ggtaagccgg
    25201 atgcaacaga tgaaatgatt tggaaagcat tagaaatcgc acaagctaaa gattttgttt
    25261 cagagatgga agatggctta gatagccatg tggaacaagg aggaggaaac ttctctggtg
    25321 gacaaagaca gcgtctggca attgcacggg cattagtcaa aacagcagat atctatgtgt
    25381 ttgatgattc cttctcagcg ttggatttca aaacggatgc caatcttcgc caagcattga
    25441 aaacgaatat gaaagaaagt atcaccgttt tagttgctca acgagtaagt acagtaatgg
    25501 atgcagatat gatccttgtg ctagatgaag gaaaactggt tggcaaagga acacatgaag
    25561 aactattagc aacaaacgaa acttatcaag aaatcgttca ttcacaacta agagaggagg
    25621 acttagcatg agtaaaacaa acacacgtcc ctctcatgga ccagcaggcg gtccaggaca
    25681 tggtggacct ggcagaaatc tcggtgtaaa aggtgaaaag cctaaaaatt tctggggaac
    25741 ggtacgccga ttgtttggct atatgtctaa acgtatggtt gcgatcatcg ctgtatttgt
    25801 actagcaata gcagccgtga tcttccaaat ccaaacacca aaggtcttgg ggaaagcaac
    25861 cacagaaata ttcaaaggtg taatggctgg cgctcaacag atgcaacaag gccaacaagt
    25921 aacaaaattt ccgatagact ttgataaaat cgcgcagatc attgcgacag taatcgtgat
    25981 gtatttgatt tctgctgtat tcaacttttt acagcaattt attatgacac gtgtttccca
    26041 acgaacagtt tatgaattac gtcatgattt ggaagccaaa atgaatcgtg taccgatttc
    26101 ctattatgat acacatacaa atggggatat catgtctcga gcaatcaatg atatggataa
    26161 catcgcaagc acgctgcagc aaaatttgac tcaatttgta actagtgcag tcacattgat
    26221 tggggtcatc gtgatgatgc tttcaatcag ctggcaattg acgttagtcg cgttattgat
    26281 gatcccttta agcttgatca tcgtgatgat cgtagcgccg aaatcacaag tgtttttcgc
    26341 agatcagcaa aaaagtttag gtttattgaa taaccaagta gaagaaacat atggtggtca
    26401 taccattgtt aaaacattca atcatactga aaaagaccag gaagtatttg aaaaagaaaa
    26461 tcagaatctg tatgccgctg gttggaaagc tcaatttatt tcggcaatca tcatgccgtt
    26521 gatgaatttc gttaaaaatc taggctatgt gttcgtcgct gtactaggcg gagtaaaagt
    26581 tgcaaatggg aacatgacat taggggatgt tcaagcgttc ttgcaatata caacacagtt
    26641 ctctcaacca atcactcaac ttgcaagttt gatgaacact atccaatcaa cagttgcttc
    26701 agccgaacgg gtatttgaag tgttggatga agaagagatg tccaatgaaa aatctggcct
    26761 tccagaagaa aaagatacac catacaaagt tcgttttgaa aatgttcaat tcggctacac
    26821 accagataac ttgctaatga ctgattttaa cttggatgtc aaacctggtg agatggtcgc
    26881 tattgtaggt ccgactggtg cagggaagac gacactgatc aaccttcttg aacggttcta
    26941 cgatgtttct ggaggtagta ttcgttacga tggtgtagat acccgtgatc tttcacgtga
    27001 tgaattgcgc gcacagtttt caatggtact gcaagatact tggctgttta caggaagtat
    27061 ctatgacaat atcaaatatg ggaacgacga tgcaacagac gaacaaatca ttgaggctgc
    27121 taaagctgct cacgtagacg attttgttcg taaattgcca gaaggatata acactgtatt
    27181 aaacgaggat gcaagcaata tttcgcaagg gcagcgtcag ctaatcacaa ttgcgcgggc
    27241 attcttagca aatccagatg tcctgatcct tgatgaagca acatctagtg tagatacacg
    27301 aacagaaata ctgattcaaa aagcgatgaa ccgcctattg gaaaaccgga caagtttcgt
    27361 tgttgctcac cgcttgtcta caatacgcga tgcagacaac attattgtga tgaatcatgg
    27421 ttcgatcgta gaaacaggaa accacgatac attgatggaa aaagacggat tctatgcaga
    27481 tctttataac agtcagtttt ctgaagaaga agcttcatag aatagtacaa atttattatt
    27541 ttttttaggg gctgtgacaa aagtcgtgtc acagtccctt tttccgaata aacggtgttc
    27601 aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgagaagc tatttttccg
    27661 ttttgtatct ctaatactca ggtcaaaccg ctgattttac aacctcttcc ttaaacggtg
    27721 ttctcctgcc aactcctcgg tcacaagtca caatagtcaa caaacctgag aagctgtttt
    27781 ttgaaattgc tccttgtgac tcggagttaa acggcaggct cacaacctct tggtaaacgg
    27841 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
    27901 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cttccttaaa
    27961 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa taggaggagc
    28021 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca acctcttttt
    28081 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
    28141 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
    28201 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
    28261 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
    28321 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
    28381 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
    28441 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
    28501 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
    28561 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
    28621 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
    28681 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
    28741 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
    28801 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
    28861 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
    28921 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
    28981 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
    29041 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
    29101 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
    29161 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
    29221 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
    29281 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
    29341 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
    29401 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
    29461 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
    29521 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
    29581 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
    29641 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
    29701 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
    29761 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
    29821 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
    29881 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
    29941 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
    30001 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
    30061 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
    30121 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
    30181 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
    30241 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
    30301 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
    30361 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
    30421 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
    30481 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
    30541 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
    30601 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
    30661 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
    30721 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
    30781 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
    30841 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
    30901 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
    30961 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
    31021 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
    31081 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
    31141 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
    31201 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
    31261 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc aaatagttcc
    31321 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
    31381 catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
    31441 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
    31501 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
    31561 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
    31621 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
    31681 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
    31741 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc catgtgaaat
    31801 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
    31861 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
    31921 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
    31981 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
    32041 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
    32101 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
    32161 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
    32221 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
    32281 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
    32341 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
    32401 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
    32461 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
    32521 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
    32581 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
    32641 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
    32701 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
    32761 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
    32821 cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
    32881 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
    32941 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
    33001 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
    33061 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
    33121 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
    33181 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
    33241 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
    33301 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
    33361 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
    33421 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
    33481 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
    33541 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
    33601 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
    33661 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
    33721 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
    33781 atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa tgagacatat
    33841 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
    33901 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
    33961 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
    34021 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
    34081 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
    34141 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
    34201 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
    34261 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
    34321 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
    34381 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
    34441 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
    34501 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
    34561 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
    34621 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
    34681 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
    34741 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
    34801 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
    34861 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
    34921 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
    34981 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
    35041 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
    35101 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
    35161 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
    35221 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
    35281 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
    35341 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
    35401 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
    35461 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
    35521 catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc aaatgatttt
    35581 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
    35641 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
    35701 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
    35761 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
    35821 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
    35881 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
    35941 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
    36001 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
    36061 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
    36121 aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat tcgtatctcc
    36181 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
    36241 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
    36301 gtaaaacgga tgctatgttt ctttaccata ctatccagta atagattgac tagagagatt
    36361 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
    36421 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagagaga
    36481 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
    36541 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
    36601 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
    36661 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
    36721 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
    36781 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
    36841 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
    36901 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
    36961 aaatctcctc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
    37021 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
    37081 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
    37141 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
    37201 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
    37261 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
    37321 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
    37381 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
    37441 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
    37501 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
    37561 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
    37621 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
    37681 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
    37741 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
    37801 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
    37861 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
    37921 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
    37981 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttcgctacc
    38041 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    38101 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    38161 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    38221 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    38281 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    38341 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    38401 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    38461 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    38521 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    38581 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    38641 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    38701 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    38761 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    38821 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    38881 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    38941 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    39001 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    39061 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    39121 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    39181 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    39241 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    39301 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    39361 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    39421 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    39481 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    39541 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    39601 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    39661 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    39721 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    39781 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    39841 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    39901 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    39961 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    40021 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    40081 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    40141 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    40201 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    40261 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    40321 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    40381 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    40441 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    40501 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    40561 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    40621 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    40681 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    40741 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    40801 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    40861 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    40921 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    40981 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    41041 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    41101 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    41161 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    41221 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    41281 gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg
    41341 cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag
    41401 gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat
    41461 gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg
    41521 tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc
    41581 attgaattct tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa
    41641 cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta
    41701 gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata
    41761 gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc
    41821 ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact
    41881 aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag
    41941 gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc
    42001 ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa
    42061 gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga
    42121 ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg
    42181 caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga
    42241 tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt
    42301 ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca
    42361 tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc
    42421 cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt
    42481 tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt
    42541 agacattata cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg
    42601 ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga
    42661 agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca
    42721 agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac
    42781 gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga
    42841 agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta
    42901 caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat
    42961 ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat
    43021 ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg
    43081 atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct
    43141 ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa
    43201 cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta
    43261 gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt
    43321 ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt
    43381 gctgacgaat aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc
    43441 ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt
    43501 aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc
    43561 tcttacctat attcccattt taaccgataa taattttctc gcttggatat tcatagccta
    43621 gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa
    43681 taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta
    43741 aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt
    43801 gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa
    43861 agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt
    43921 tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc
    43981 gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga
    44041 tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg
    44101 taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat
    44161 ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta
    44221 acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt
    44281 ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca
    44341 aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag
    44401 tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg
    44461 catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa
    44521 aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag
    44581 acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc
    44641 gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa
    44701 ataatatttc cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg
    44761 atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat
    44821 ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca
    44881 atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc
    44941 ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat
    45001 agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat
    45061 aacaatacag ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa
    45121 gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc
    45181 aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag
    45241 taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac acattggaat
    45301 tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa
    45361 gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa
    45421 ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg
    45481 ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca
    45541 atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa
    45601 gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata
    45661 tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg
    45721 aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg
    45781 tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt
    45841 acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta
    45901 agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa
    45961 agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct
    46021 attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt
    46081 gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca
    46141 ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact
    46201 acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact
    46261 gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat
    46321 ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta
    46381 gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga
    46441 aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta
    46501 tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg
    46561 gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt
    46621 ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt
    46681 attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca
    46741 ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga
    46801 tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg
    46861 aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca
    46921 aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt
    46981 attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact
    47041 cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg
    47101 tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt
    47161 atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc
    47221 gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata
    47281 aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata
    47341 taacgagaaa gataagacga acacatcact tattctttcc tttcatttat aaacattgta
    47401 gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca
    47461 agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta
    47521 gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg
    47581 atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa
    47641 cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg
    47701 atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc
    47761 aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta
    47821 caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt
    47881 acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc
    47941 ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa
    48001 gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag
    48061 aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg
    48121 agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg
    48181 aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga
    48241 caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt
    48301 tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag
    48361 ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa
    48421 gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga
    48481 tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag
    48541 tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa
    48601 gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag
    48661 tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt
    48721 tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa
    48781 ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg
    48841 agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg
    48901 tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa
    48961 atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt
    49021 aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg
    49081 ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa
    49141 ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac
    49201 tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt
    49261 tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa
    49321 aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg
    49381 ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa
    49441 ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca
    49501 cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga
    49561 gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt
    49621 gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg
    49681 atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac
    49741 gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt
    49801 gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt
    49861 tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat
    49921 gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat
    49981 tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa
    50041 ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata
    50101 caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt
    50161 atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa
    50221 aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa
    50281 aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata
    50341 aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg
    50401 gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga
    50461 tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag
    50521 acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt
    50581 caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag
    50641 ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt
    50701 caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac
    50761 caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag
    50821 caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt
    50881 atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt
    50941 ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag
    51001 atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa
    51061 catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg
    51121 gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg
    51181 gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa
    51241 gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag
    51301 tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg
    51361 atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta
    51421 ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca
    51481 gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg
    51541 aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga
    51601 ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat
    51661 gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct
    51721 gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa
    51781 tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata
    51841 tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa
    51901 cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat
    51961 tataaaaaaa cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta
    52021 tccactattg acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca
    52081 aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga
    52141 aacagaattg caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac
    52201 cgaaacagta tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt
    52261 ttctgtaaaa gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca
    52321 agtaaaagag tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg
    52381 gcctggacca ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac
    52441 gggaggatta tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat
    52501 cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc
    52561 aacgactgca gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga
    52621 tggagctacg cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat
    52681 gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc
    52741 tcctgttgct attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat
    52801 gaaatacaaa cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc
    52861 aactgcagtt caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga
    52921 aattgcggat caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt
    52981 agaagcggcg gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg
    53041 attagatttg atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa
    53101 tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg
    53161 cattctttta gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta
    53221 agcgtaacta atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa
    53281 aataaaattg taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag
    53341 attacaaaac gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag
    53401 aacataattt ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac
    53461 aaggaagtat cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg
    53521 ggtgtgaatt tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg
    53581 gagcgaaatt tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc
    53641 tcgctttggt agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc
    53701 taacacatgg atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg
    53761 ttgatccgac aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc
    53821 aaccaaaatt gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat
    53881 tcagagaaat cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag
    53941 gattagtagc agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta
    54001 ccactcataa aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat
    54061 tagcaaaaaa aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg
    54121 tgattgcggg taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata
    54181 gtgaacaaat catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa
    54241 ccagagtcat ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag
    54301 gtataaatgg aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa
    54361 actcgattcc ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc
    54421 cagcaatcac tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg
    54481 tcaaagcatt acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac
    54541 gcgaattaac tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa
    54601 cagaaaagtt caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa
    54661 tatcggtgat gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg
    54721 aggaaaagtc atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc
    54781 aaatgcgcct catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga
    54841 agaagtacaa gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga
    54901 tactggcgaa tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa
    54961 attaccagtt attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca
    55021 tgataaattg atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata
    55081 tagtcgatca ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca
    55141 acagctgaat aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg
    55201 agattcagta ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg
    55261 cctcccaata tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga
    55321 ttcctatcat gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg
    55381 ggaccaatat acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc
    55441 aaatataaat gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt
    55501 attaagagat gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac
    55561 acaagtacgc ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac
    55621 aaaggcaacg cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc
    55681 tttgcttgtc cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag
    55741 tttgactagt caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga
    55801 gagcttaaaa aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa
    55861 ctattattcg ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg
    55921 ctatgttgat ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa
    55981 caccgatcac tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa
    56041 tcttgcgcgc aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag
    56101 tgggacatat cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca
    56161 aacttccaga agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag
    56221 gcggatcagc aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat
    56281 ttgtttgttt ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata
    56341 ttgacatcta tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg
    56401 gactagggga tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg
    56461 tgacacaact tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt
    56521 tttcacagct ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt
    56581 caaatctttt ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag
    56641 ttcgcatttt ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa
    56701 atcgttccag tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat
    56761 ttttcaccaa tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc
    56821 ataaaacctg cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa
    56881 ctaattttcc atcggactct ttacaatcag ccaaatagag tatataattt cttttggata
    56941 aggccagctt agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga
    57001 tccctatagc tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata
    57061 catctgtctt tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta
    57121 tttctataaa aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca
    57181 catgaactgc ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca
    57241 atcttgccac cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat
    57301 cataagtact gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa
    57361 tgctgaactt actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta
    57421 tcatgatagg caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg
    57481 ttaaacccca tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat
    57541 tgataaaaaa atatttggct atatttttga caggagcgac aggtttcgag aacatttttt
    57601 tcgtatcttt tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa
    57661 aaatgctaat aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag
    57721 tgcccaatat ccc
//
LOCUS       DAYYKC010000007        54639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000007 DAYYKC010000000
VERSION     DAYYKC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.007"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..1732
                     /locus_tag="ACHBBW_000444"
     CDS             209..1732
                     /locus_tag="ACHBBW_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9152156.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1969..3309
                     /locus_tag="ACHBBW_000445"
     CDS             1969..3309
                     /locus_tag="ACHBBW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9152157.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3462..4199
                     /locus_tag="ACHBBW_000446"
     CDS             3462..4199
                     /locus_tag="ACHBBW_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9152158.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4364..4585)
                     /locus_tag="ACHBBW_000447"
     CDS             complement(4364..4585)
                     /locus_tag="ACHBBW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9152159.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4616..5647
                     /locus_tag="ACHBBW_000448"
     CDS             4616..5647
                     /locus_tag="ACHBBW_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152160.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5634..7037
                     /locus_tag="ACHBBW_000449"
     CDS             5634..7037
                     /locus_tag="ACHBBW_000449"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9152161.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            7103..7738
                     /locus_tag="ACHBBW_000450"
     CDS             7103..7738
                     /locus_tag="ACHBBW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9152162.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7787..8467
                     /gene="cmk"
                     /locus_tag="ACHBBW_000451"
     CDS             7787..8467
                     /gene="cmk"
                     /locus_tag="ACHBBW_000451"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9152163.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            8602..9831
                     /gene="rpsA"
                     /locus_tag="ACHBBW_000452"
     CDS             8602..9831
                     /gene="rpsA"
                     /locus_tag="ACHBBW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9152164.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            10013..11323
                     /gene="der"
                     /locus_tag="ACHBBW_000453"
     CDS             10013..11323
                     /gene="der"
                     /locus_tag="ACHBBW_000453"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9152165.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            11566..11841
                     /locus_tag="ACHBBW_000454"
     CDS             11566..11841
                     /locus_tag="ACHBBW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9152166.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            12076..13122
                     /locus_tag="ACHBBW_000455"
     CDS             12076..13122
                     /locus_tag="ACHBBW_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152167.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            13124..14383
                     /locus_tag="ACHBBW_000456"
     CDS             13124..14383
                     /locus_tag="ACHBBW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9152168.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            14389..14970
                     /locus_tag="ACHBBW_000457"
     CDS             14389..14970
                     /locus_tag="ACHBBW_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9152169.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(15052..15927)
                     /locus_tag="ACHBBW_000458"
     CDS             complement(15052..15927)
                     /locus_tag="ACHBBW_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9152170.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            16075..16407
                     /locus_tag="ACHBBW_000459"
     CDS             16075..16407
                     /locus_tag="ACHBBW_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9152171.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            16519..17727
                     /locus_tag="ACHBBW_000460"
     CDS             16519..17727
                     /locus_tag="ACHBBW_000460"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9152172.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            17881..18336
                     /locus_tag="ACHBBW_000461"
     CDS             17881..18336
                     /locus_tag="ACHBBW_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152173.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            18698..18973
                     /locus_tag="ACHBBW_000462"
     CDS             18698..18973
                     /locus_tag="ACHBBW_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152174.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            18986..21073
                     /locus_tag="ACHBBW_000463"
     CDS             18986..21073
                     /locus_tag="ACHBBW_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9152175.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKNESNKIKR"
     gene            21228..23111
                     /locus_tag="ACHBBW_000464"
     CDS             21228..23111
                     /locus_tag="ACHBBW_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9152176.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            23123..24070
                     /locus_tag="ACHBBW_000465"
     CDS             23123..24070
                     /locus_tag="ACHBBW_000465"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9152177.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            24090..24617
                     /locus_tag="ACHBBW_000466"
     CDS             24090..24617
                     /locus_tag="ACHBBW_000466"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9152178.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(24680..25333)
                     /locus_tag="ACHBBW_000467"
     CDS             complement(24680..25333)
                     /locus_tag="ACHBBW_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9152179.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            25465..26307
                     /locus_tag="ACHBBW_000468"
     CDS             25465..26307
                     /locus_tag="ACHBBW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9152180.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            26465..27361
                     /locus_tag="ACHBBW_000469"
     CDS             26465..27361
                     /locus_tag="ACHBBW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9152181.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            27364..28077
                     /locus_tag="ACHBBW_000470"
     CDS             27364..28077
                     /locus_tag="ACHBBW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9152182.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            28097..28615
                     /gene="msrA"
                     /locus_tag="ACHBBW_000471"
     CDS             28097..28615
                     /gene="msrA"
                     /locus_tag="ACHBBW_000471"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9152183.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            28612..28839
                     /locus_tag="ACHBBW_000472"
     CDS             28612..28839
                     /locus_tag="ACHBBW_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9152184.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            28991..29542
                     /gene="lepB"
                     /locus_tag="ACHBBW_000473"
     CDS             28991..29542
                     /gene="lepB"
                     /locus_tag="ACHBBW_000473"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9152185.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29625..30167)
                     /locus_tag="ACHBBW_000474"
     CDS             complement(29625..30167)
                     /locus_tag="ACHBBW_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9152186.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(30404..30802)
                     /locus_tag="ACHBBW_000475"
     CDS             complement(30404..30802)
                     /locus_tag="ACHBBW_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9152187.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            31062..31922
                     /gene="ylqF"
                     /locus_tag="ACHBBW_000476"
     CDS             31062..31922
                     /gene="ylqF"
                     /locus_tag="ACHBBW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9152188.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            31915..32682
                     /locus_tag="ACHBBW_000477"
     CDS             31915..32682
                     /locus_tag="ACHBBW_000477"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9152189.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            32738..33595
                     /gene="dprA"
                     /locus_tag="ACHBBW_000478"
     CDS             32738..33595
                     /gene="dprA"
                     /locus_tag="ACHBBW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9152190.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            33717..35795
                     /gene="topA"
                     /locus_tag="ACHBBW_000479"
     CDS             33717..35795
                     /gene="topA"
                     /locus_tag="ACHBBW_000479"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9152191.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            35764..37140
                     /gene="trmFO"
                     /locus_tag="ACHBBW_000480"
     CDS             35764..37140
                     /gene="trmFO"
                     /locus_tag="ACHBBW_000480"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9152192.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            37338..38243
                     /gene="xerC"
                     /locus_tag="ACHBBW_000481"
     CDS             37338..38243
                     /gene="xerC"
                     /locus_tag="ACHBBW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9152193.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            38280..38828
                     /gene="hslV"
                     /locus_tag="ACHBBW_000482"
     CDS             38280..38828
                     /gene="hslV"
                     /locus_tag="ACHBBW_000482"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9152194.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            38842..40242
                     /gene="hslU"
                     /locus_tag="ACHBBW_000483"
     CDS             38842..40242
                     /gene="hslU"
                     /locus_tag="ACHBBW_000483"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9152195.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            40260..41051
                     /gene="codY"
                     /locus_tag="ACHBBW_000484"
     CDS             40260..41051
                     /gene="codY"
                     /locus_tag="ACHBBW_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9152196.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            41133..42008
                     /locus_tag="ACHBBW_000485"
     CDS             41133..42008
                     /locus_tag="ACHBBW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9152197.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(42140..42778)
                     /gene="plsY"
                     /locus_tag="ACHBBW_000486"
     CDS             complement(42140..42778)
                     /gene="plsY"
                     /locus_tag="ACHBBW_000486"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9152198.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            42905..43339
                     /locus_tag="ACHBBW_000487"
     CDS             42905..43339
                     /locus_tag="ACHBBW_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9152199.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            43505..45550
                     /gene="parE"
                     /locus_tag="ACHBBW_000488"
     CDS             43505..45550
                     /gene="parE"
                     /locus_tag="ACHBBW_000488"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9152200.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            45562..48012
                     /gene="parC"
                     /locus_tag="ACHBBW_000489"
     CDS             45562..48012
                     /gene="parC"
                     /locus_tag="ACHBBW_000489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9152201.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            48289..50520
                     /gene="pflB"
                     /locus_tag="ACHBBW_000490"
     CDS             48289..50520
                     /gene="pflB"
                     /locus_tag="ACHBBW_000490"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9152202.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            50764..51525
                     /gene="pflA"
                     /locus_tag="ACHBBW_000491"
     CDS             50764..51525
                     /gene="pflA"
                     /locus_tag="ACHBBW_000491"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9152203.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            51610..52536
                     /locus_tag="ACHBBW_000492"
     CDS             51610..52536
                     /locus_tag="ACHBBW_000492"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9152204.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            52718..53611
                     /locus_tag="ACHBBW_000493"
     CDS             52718..53611
                     /locus_tag="ACHBBW_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9152205.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            53745..53870
                     /locus_tag="ACHBBW_000494"
     CDS             53745..53870
                     /locus_tag="ACHBBW_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9152206.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(54004..54636)
                     /locus_tag="ACHBBW_000495"
     CDS             complement(54004..54636)
                     /locus_tag="ACHBBW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152207.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        18899 a         9314 c        11547 g        14879 t
ORIGIN      
        1 tgtttgttta taaaaaaaag gaaacgctac aatgaaaaag aagaaagctt taaaaacctg
       61 aaaaaagagg agggaatagg gaaaaacgaa ctttttgaac aaaagtttta caaaatgcgt
      121 gttattattt ttgttctgat actctttttg ataaaataac attagagcca aaattgttgt
      181 ctctaacgga aaagaaagga agaagaagat ggaacaaaca aaaaagaaaa aatttcaaat
      241 gccttcagcg tatacggtct tatttattat tattgcagta atcgctgtac ttacttggtt
      301 tataccagct gggcactata gtgtagacga tgcaggcaac atcgtcgctg gatcatatga
      361 acgagtacaa caaaatcctc aagggctgtg ggatattttc atggcgccaa tcaatgggat
      421 gctaggtgta ccagcggggg agctaactaa agaaacacct gctgcaatcc caatctcttt
      481 cttcattttg gttgttggtg gatttttagg tattatcaac aaaacagggg cactagatgc
      541 cgggattgct tcagttgtga agaaattcaa aggaaaagaa aaaatgctta ttccagtatt
      601 gatgctgtta tttgcattag gcggctctac ttacggaatg gcagaagaaa cgattccatt
      661 ctatgcttta ctgatcccag tcatgatggc tgtaggcttt gatacagtag tagctgtagc
      721 aatcgtatta gtagggtctc aagtaggctg tctagcttca acggtaaatc cttttgcaac
      781 aggcgtagct tcacaagctg ttgggatttc gatgggtgaa ggtattggtc ttcgattctt
      841 gatgttcgtt attttgttag ctatttctat tgtatatgtg tatcgttatg catcaaagat
      901 cgaaaaagat ccaacaaaat cattagtata caaccaacgt aaagaagata tgaagcattt
      961 cgatattgaa gcaatcggtc gtcaagaagt aatcacgaaa aaacaaaaac atgttaatgt
     1021 attgttcttt attacttttg gtatcatgat catcagtttg attccttgga caaccttgaa
     1081 tgcgaatttc acgatttttg aatcattgac aaactggtta acaaaccttc cggtactagg
     1141 tacggttcta ggtaaaagca tgcttccatt aggtgactgg tacttcccag aaattactat
     1201 gttattctta gtaatgtcca tcgtagtagc aatcacttat ggcatgaaag agtctgaatt
     1261 tgtctctgaa tttttagctg gtgcttctga tttgatcggt gtagcaatcg tagtagctgt
     1321 tgctcgtggt atccaagtgg ttatgaacaa tggattaatc acagacacca tcttacattg
     1381 gggcgaacaa ggattaagtt cattatcttc aagtgtattc atcattatta catttatttt
     1441 ctatatccct atgtcattct tgattccttc aacttccggc ttagctgctg caacaatggg
     1501 aatcatggga cctatgggtg aatttgccgg cgtcggtaag gatttagtaa tcactgctta
     1561 tcaatctgca tctggtttag taaacttaat tacaccaaca tctgctatcg taatgggtgc
     1621 tgtagcaatt gctcgtttcg atgtttcagt gtggtggaaa tttaccggta aattaattgc
     1681 gatattattt gtagccatct gtgtgatcct aggtgtagca gcaatgttct aaacctagac
     1741 aaatacaggt acaaggtcta tcataagaat gaagaaaccg ataaacggct atcgataagc
     1801 acgtggataa gtcaatagct ccattcagtg agtgtttttt ctttcatcat ttgttaagaa
     1861 cattctgctt gctgatggag caggcttaaa acgtcattat ttgagaagaa gaggttgtgt
     1921 cggaggaatt tgacatgacc ctttttaaca tattaggagg ataatatcat gaaaaaattt
     1981 gtgacagaac aacatcatga acaagcatta gaatcattga gcgaattgat ccgaattcct
     2041 tccgtactag atgaagcaga ttcaggacaa ggacatccct tcggtaaaaa agtgattgaa
     2101 gctttggata aagtattgga aatcagtgaa aagctaggat ttagaacatt taaagatcca
     2161 gaaggctatt acggttattc agagattggc tctggagatg aattattcgg tatcttgtgt
     2221 catatggacg ttgttccagc aggggacgaa aataattggg aaacaaaacc attcgatcca
     2281 actataaaag acggctggtt agttggtcgc ggatcccaag atgacaaagg tccttctatt
     2341 gcggcaatgt atgccgtcaa agctttgatt gatgctggcg tggaattcaa gacacgtatt
     2401 cgttttatct ttggtacaga tgaagaaaat ttatggcgtt gtttagaaaa atataacgaa
     2461 aaagaagaag gaattacgca aggatttgcg ccagatgctg aatttccttt aatttatgca
     2521 gaaaaaggct tattgcaagc ttacttgaca ggaccaggaa cgaacgagtt ttctgtaaaa
     2581 gctggaggag cgttgaatgt tgttcctgat gcagctcctt actctggaga aaagctttct
     2641 gaagtaaaag aagcattgaa aaaacacgaa ttcgatttcg aagaacaagg tgaaggcatc
     2701 gtcgttcttg gtaaaagtat ccacgcgaaa gatgcagcac aaggagtgaa tgctatttct
     2761 cgtctggcaa ttgctctttc agaagtattt gattttggcc caatcaattt cttaggaaaa
     2821 ttagttcaag aaaatgcaac tggtgaagct gtcgtgggca aaacagaaga tgaacaatcc
     2881 ggcgagctaa cgatgaactt tgcaagtttg gaaatcacac cagaacaaac aaaaatcggt
     2941 gtagatatga gaatccctgt tacattcaag aaagatgatt tagtagctaa attaactaaa
     3001 acagcagaaa aatatgggtt gacttatgaa gaatttgact tccttgattc attatacgtg
     3061 ccactagaca gtgagttagt gaaaaatcta ttggggacat atcgcgatat tactggagac
     3121 atgactgaac cgtttgtatc aggcggcgct acttttgctc gtacgatgaa ccagtgtgtg
     3181 gcattcggtg cgatgttccc tgatactcct gactttatgc accaggcgaa tgaacgatgg
     3241 gaattaagta gtatgtataa agcaatggaa atttatgcag aagctgtgta tcgtttatgt
     3301 gctaaataaa aataaacata caaaaagggc ggatattcaa atggatattc cgtctttttt
     3361 tatttgatgc tggaaagttt gcatttctct cttatttttt taaggtataa tcatacttga
     3421 ttaagaaaaa ggggtcactc agatagaaaa ggtgggagaa agtgaaaata tttagacgaa
     3481 tactaattgg cttagtcatc attttaggat tactcggtgg agccggttat tactatatac
     3541 agcaaaatac gtacgaacca tcgtcagaag cgcaagtgtt ggtaaaagaa ggaaaaaaca
     3601 aagaagatta cttatttttt gaaggagaga aaaagaatca tggaaagtct ccattactta
     3661 ttttttatcc tggtgctctc gtagagccag aaagttatag tatttgggcg caagatttag
     3721 caaaaaacgg cttttcagtc gctattgtca aaatgccact tgatctagca gtgatgggcg
     3781 gaaacaaagc ggataaggtc aaagaagatt ttccaaattc cgattatgtg atcggtggcc
     3841 attcattagg tggcgtaatg gcaagtcgtt atgtcaataa gaatcattca gacaaacatt
     3901 taaaaggggt attcttcatg gcaagttatc ccgacaaaaa aggaacattg acagaatcag
     3961 ctgttcctgt tttatcgatc acggcgacaa aagataaagt attgaatcag gaagcttatc
     4021 aagaagctaa gcagtattta ccggaaagaa cagagtatgt atcaatcaat ggcggaaatc
     4081 atggaggttt tggcagctat ggtgagcaaa aaggagatgg agaggcagaa atttctaacg
     4141 cagaccagca aactgtgatc gtcaatacat tggaaaactg gctgaaaaag ttgaaataat
     4201 ataaatgggt cctatagggt ccgtgaaaaa actagtcaat taactataga tagcaaatga
     4261 atctttcaaa ataagtcggg atttcacgga acaagagaaa cagatcccgg cttattttca
     4321 agtttagaga acttagtgta cgtttttaaa gtttcgttta tatttatatt ttattttcta
     4381 ataaaatcgc tcgaactgga catttttgat acgctttttt tacatatgtt tcttcttccg
     4441 gcatcacgaa ctgttgatga gcctctggtt cttgggcaaa taaaacgatt ccttcttcat
     4501 cataatcgaa aatatctggc gcttcgattt gacaaagacc gcatgcaata cagcgttcag
     4561 gtacaagttt acatagtagt gacatttcta accatcctaa cttaaggaga ataatatgat
     4621 tgtttttatt ttatcgctat tttcatcagg acacaagctg cggattagtt ctctttacca
     4681 actccttgtg ggaaaacgaa cgacttcagt acttatttat ggatttaccc acgaactttt
     4741 gtttatccat aacagttttc cagaattgaa gcaggataag ttttatcaaa ttttgcaaaa
     4801 aattgcgcag caaggttgga tcgaaataaa tgaaaatgaa gcaaagctaa cgcctgcagg
     4861 ggctgatatg ttgtccgaac atcaaataga acatactggt ttgcgttttg accgctatgg
     4921 cagaacaggg gaaactagtt ggcggctcat taaatttgct gttcaagtaa tttctaatct
     4981 ggcaacgggt attcaagact atcttccagc agagacaagt cctttttata ctttccaact
     5041 aaaaaaatgg cttagtgaaa gccaattgcc acgtgaaatt ttgattgata ctgcgtatga
     5101 gagtttggtg cagctgtttt cggagatacc agaagaagca gcagattttt tagctaacca
     5161 attatctgga aatgagcgaa cagggctgct tccttatcag ctggcaaaaa acaatgatga
     5221 gtcagcggtt tatctgcaac aaagcagatg tatccacctt ttgttagcac aaatcgaaaa
     5281 acggccagat tcgctttggc acgctttgat cgattcatta ttacagcaaa atttcaatca
     5341 aagcatgatg atcaccaagc agatgtttat gaatggtcag acaattgacc agatcatggc
     5401 aatccgtcat ttaaaaagag gaacagtaac agatcatctt attgagtggg ccttgttttt
     5461 tgatgatttt ccatacgagt ggattctctc tgcagaaaca atcgaacgtt tggaaccaaa
     5521 taaggattcc gtgcgagagt ggcgctttag cgaatggaat gtagatggac aattagatta
     5581 tggagaattt cgtttgtatc aaatctatct attgagaaag gaagcgatac agaatgttaa
     5641 taaatgaatt aaaaaaatat tttggatttg attcatttcg tcccggccaa gaagaaatta
     5701 ttcaggcgtt gcttgatgga gaagatactc tcgctatttt gcctacagga acgggaaaaa
     5761 gcctttgcta tcaattgacg gggtatttaa tggaaggtct ggtcatcatc gtgtcaccgc
     5821 tgctttctct aatggaagat caagtgacgc aattacaaaa acgaggggag aaacgagtag
     5881 ctgctttcaa tagtttactt tcgcaatctg aaagaagata cgtcctccgt catctatcgc
     5941 aatataaatt tttgtttttg agtcccgaaa tgttgactaa tccttctctt ttagagcatc
     6001 tgaaaaaaca aaatattgcg ttatatgtag tagatgaggc tcattgcgtg tctcaatggg
     6061 gagttgattt ccgtccagaa tatcaacagt taggaaaaat ccgaaagctt ttaggaaacc
     6121 cggtcacact ggcactaacg gcgacagcaa cagatttagt agcaagagat atacgtcatg
     6181 tactatttga tcgaccacca aaagagatca gacaatcagt caatcggcaa aatatctcgc
     6241 tttttgttcg gaaaacgcag caaaaagaac aagaacttga acacttcatg gaaagagcac
     6301 aaggagcggc aatcatttat tgtgcaacaa aaaaagaagt cgaacgattg tatcatttat
     6361 tcagggaacg ttttacagtt ggatactatc acggtggtct tgatgcagct caaagaagac
     6421 aacttcaaca gcagtttgta aaaaatcagc tgcagtttct cattgctacg aatgcatttg
     6481 gtatggggat cgataaatct gatatccgtt atgtggtcca ttatgatttg ccagacagtc
     6541 tagaaaacta tgtacaagaa atcggtcgag caggcagaga tcaggaagaa agtgcagcaa
     6601 ttctttttta tcaaagtggg gatgaacgta tccattattt ctttaatcaa ctttctagag
     6661 aacagcgaca aagttttgag ctttatttgg aatatgccac agagcaagct ccctttgatg
     6721 agctgcaaaa aaaatggatg gagctgatcc agcagtctga aaaaccagaa aattgggtgg
     6781 aacggctcaa acgtcaggaa aaagaaaaag aattccgcct gcagcagatg cttcgctata
     6841 ttaatgaaga aaactgccgc agaaaattca tcttagctta ttttggcgaa aaactctccg
     6901 aaaagccgca gaattgttgt gatattgatg gagcaaaagc agaacttctg ccaaagagga
     6961 agcttttttc taagactgaa caacttcatt ggggaacgat tttactaaat ttatttaaaa
     7021 aaaacatgga acattgattt tttaggacaa gacacttttt cctatggtat aatacacacg
     7081 atagagttta ggaggtaaaa cagtgagcag aaaagataga catcaatcta cagaaaataa
     7141 cgaaacacaa gaaccatggg aacaaccaat ctacgataca gatgatgaag tttcatcaag
     7201 aagttctcag agacaacaaa agaaaggaaa tacattattt ctttcgctat ttttgatttt
     7261 actggtctta tgtatcgcta ttccagcagg tgcttatttc tggatcagaa atggatcaag
     7321 caacaatgca acagcagcaa cttcttctga aacttcgaca tcagtagtag aaagttctac
     7381 aagtacaaca aaagaggagt caactgagac ttcaacagta gaatctagta caatcgcgga
     7441 agaatctact gtgtctcaag atacaggtgt aacgtcagaa agcagtattg cagcagagac
     7501 aacaccgact tcctcaagtg aggcaactta tcaagcaggg gattcaagca gcaatgcagc
     7561 agaaacaaca ccttcttcat cagctgcagc cggttcaaca acgactgttc aagcaggcga
     7621 aggtccaaaa caagtggctg agcgtgcagg gatcaccact gatcaattgt ttgaattgaa
     7681 tggcttagat ccaaacaact ttatgcttta ccctggtcaa gaattaagaa ttaaataata
     7741 atagccgcgc tttgcggctt tttttctgag aaaaaggagt tatatcatga cacaaatcag
     7801 tattgcaatc gacgggcctg cttcatctgg gaaaagtacc gttgccaaga tattagcaaa
     7861 agaattgaat tatatttata cagataccgg tgccatgtat cgcgcagtaa cttatttagc
     7921 tattacgaac aatgtatctt tttcagatga agaagggcta gtttcgctga tcaaaaagta
     7981 tcctatctct ttttcgcaac aagaagatgg acagcatgta ttcatagcat caaaagatgt
     8041 gactttagaa atccgtcaac cagatgtcac aaaagctgtt tctgaagtct ctgcacatgg
     8101 aaaagtcaga gaagaattag ttgctgtcca acgaaaaata ggggaacgcg gtggcgttgt
     8161 catggacgga agagacatcg ggacagctgt attaccgaat gcagaagtga aaatcttttt
     8221 agtagcaagc gtaagtgagc gtgcagaaag acgtttcaaa gagaaccaag aaaaaggaat
     8281 tccgactgat cttgaaacac tgacgaaaga gatacaggaa agagaccatt atgattcaac
     8341 aagggacgtc tctccgctga agcaagcaga ggatgctgtt cgtatcgata cgactggcaa
     8401 atcgattcca gaagtcgttg cagcaataaa agctgttgtt ttgaaaaaag gttatcttct
     8461 tttttaaatt aagaaaaaaa atgaaaaaca attgtttttt caaggaaaac gaacgaaatc
     8521 gctttatttt ttttgaataa tcaattaaac taaaaacagt accgtactta cggcagaatg
     8581 ttggttagga ggacaagtgt tatgacagaa tttgatcaaa atcaagagaa caaccagtca
     8641 atggaagacg caatgaaaag cgtacaagaa gtaaaagttg gcgatgttgt taagggcgaa
     8701 gttttagtca ttgaagacaa acaagtgatt gtcggaatcg aaggggctgg agttgaagga
     8761 gtcgttccgg caaaagaatt atcaactttg ccggtagaag atatcagcga agtagtaaaa
     8821 gtcggtgatg tattagattt agtcgttatc agtacgatcg gcaaagataa agaaaacggc
     8881 agctatctac tttcaaaacg ccgtttagat gctaaaaaag tttgggaaga tatcgaaaga
     8941 gatttccaag agggcaaggt catcgaagca cctgttacaa atgttgtcaa aggcggttta
     9001 gtcgttgacg tcggtgtacg tggttttgtc cctgcatcaa tggtggaaga tcattttgtt
     9061 gcagactttt cagactataa aggcaaaaca ttgagcttta aaatcatcga aatcgaacca
     9121 tcagaaaatc gtttgatctt atcgcataaa gcggtagtgg aaaacgaaag agaagaaaag
     9181 aaaaaagaaa ttctttcaac tatccatgag ggagatgttc tcagtggacg tgttgcacgt
     9241 cttactgatt ttggcgcatt tgtcgaccta ggcggaattg acggtctagt ccatgtctca
     9301 gagatttcac atagtcatgt agcaaaacca agtgatgtat tggctgtcaa tgatgatgta
     9361 aatgtcaaag tgttgtctat caatcctgaa caagaacgtg tttccttgtc aatcaaagac
     9421 acacagcctg gaccttggtc agaaatcgat acaaaagctc cagttggcac agtattagac
     9481 ggaacagtta aacgtttgac aagcttcggt gcctttgttg aagtgttccc tggagtagaa
     9541 gggctagtcc atatctctca aatttcacat aaacatattg cgacaccaca tgaagtgctt
     9601 catgaaggtg acgaagtgaa agtgaaagta ttagagatcc atccagaaga acatcgtatt
     9661 gcattgagta tcaaagcttt agaagaaaaa ccagcttcag aagaagaacc aaaaaatgtt
     9721 gagtcttatg aattaccaga agaaaatact ggatttacta tgggagacat tttaggtgat
     9781 gcgttgaaag aagaagtaga agaaaaagca accgatgatt ctgaaaaata attgattttg
     9841 tatctgtgac tgagatgaaa gcagctgctt ttgtctcagt ttttcttttg aaaagcgatt
     9901 agtatttcaa taaggaataa aaatacttcg tttaattgac aatctatgct tgaaagttgg
     9961 caatgcattc gttaaactaa gtgagattgg aaacttgtga ggaggaaata atatggcaaa
    10021 tccaacaatt gcaatcgttg gccgcccgaa cgtcggtaag tctacgattt ttaaccgcat
    10081 cgctggtgag cgtatctcga ttgtcgaaga tactcctgga gtaactcgtg atcgtattta
    10141 tgcaaaaggt gaatggttag gtcgagaatt cagcgtgatc gacacaggcg gaattgatct
    10201 tggcgatgaa ccttttatgg atcagatcaa gcatcaagca gaaatcgcga ttgaagaagc
    10261 agatgtaatc atttgtgtag tcagcggacg cgaaggcgtg acagacgcag atgagatggt
    10321 tgcgaaaatt ttatacagaa gtaataaacc agtgatttta gcagtcaata aagtagataa
    10381 tccagaaatg agaaacgata tctatgaatt ttattcctta gggctaggcg atccttatcc
    10441 tgtttcagga agccatggac tagggattgg tgatatttta gatgaagcag tgaagtattt
    10501 ctctgaagag actgaagaag aggacgacga cacgattaag tttagtttga ttggtcgccc
    10561 aaatgttggt aaatcctctt tgatcaatgc gattttagga gaagaacgtg taattgtttc
    10621 ggatattgaa ggaacgaccc gagacgcaat cgacacccac ttcgtttctg aaagtggtca
    10681 gaaattctta atgatagata cagctggtat gcgtaaacgc ggaaaagtat acgaaaatac
    10741 tgaaaaatac agcgtgatgc gtgcaatgcg tgccattgac cgttctgata tcgttttgat
    10801 ggtactgaat gctgaagaag ggattcggga acaagacaag cgagtagcgg gttacgccca
    10861 tgaagctggt cgtggaatta ttatcgtcgt gaataaatgg gatcttgtaa agaaagaaac
    10921 caatacaatg cgtgatttcg aacaagagat tcgtgatgaa ttccggtatc ttgattacgc
    10981 tccaatcgtt tttgtttctg cagtgactaa acaacggttg gaacgtctgc ctgaaatgat
    11041 cgaacaagta agtatgaacc agaatcttcg tatctcttcg gctgtgttga atgatatcat
    11101 tatggatgct gtagcaatca atccaacacc aacagataaa ggaaaacgcc tgaagatttt
    11161 ttatgctaca caagtcgctg tcaaaccacc tacatttgtc gtttttgtca atgaagagga
    11221 attgatgcat ttttcttatg aacgtttctt agaaaatcaa atcagaaaag catttacttt
    11281 tgaagggacg tccatccgaa ttatccctcg taggagaaaa taggcatttt accatatcga
    11341 cgtttttttt tcaaaagaaa aagacagttt cgtttgttac gtttctatga ctaaaaaaag
    11401 catgtgaaaa ttggtaaaaa ccttgctatt aagcggttca tatgatatcg tgataacaga
    11461 atattgaagt taatcaatat ttctttttaa acttctggac cactcagaaa gttttaacta
    11521 aatgatattt ctatcatatc tcttgtggag gaggtgaatc tatccatggc aaacaaagca
    11581 gaattaatcg aaaaagttgc ttcagctact gatttaacta aaaaagatgc aactgcagca
    11641 gtagatgctg tattttcaac tattcaagat gctttagcta acggcgaaaa agttcaatta
    11701 atcggttttg gtaactttga agttcgcgaa cgcgcagctc gcaaaggtcg taacccacaa
    11761 actggtgaag aaatcgaaat tccagctagc aaagtacctg cattcaaacc aggtaaagca
    11821 ttaaaagacg ctgttaaata agacagttga aaaagcgctt gtggctctag ggctacaagt
    11881 gcttttcttt ttatttccta gcagaaaagg aataaataag agacagagat tccgaatccg
    11941 tatgtcatac atagaaaaca catgtatttt atttaatttt tatttatttg caagttatga
    12001 ggtgagcttt gctcttatct tttctagaat ttgcttacta atggtaaaat aaaaataaga
    12061 agaggaggga tgcgtatgga acttgattat caagtagaag atgcgttgac gaaagtgttt
    12121 tacggagaaa cgctagtcag tcagctaaga gaactttcca ttgatcagcc aatcttattt
    12181 ttaacgaatc agcgctatta tgatttgttt gcagataaga tcaacccgtt atttgcaaat
    12241 caagcaaaca atgactggta tatttgtgcc aacactcaat gcaatcattt aacagaatta
    12301 aagaacctgc ttgatttcgt caaacgctat cccgaaaatc aatccatgtt tatcatcgga
    12361 tttggaaatg aagggatcat ggatctggca ggtttttttc aaaaacatac aacccttgat
    12421 tcatcactat ggatgatccc tgtatcgatc cgttctctaa gccgagcatt aacaccgcag
    12481 cggacgattt tgcagctacc aaatcgtgta gtgctgcaaa ccatcaatct gccagaacgc
    12541 atcgtttatg atcaaacaat cagcgagcgg caggtggacg gaaagcagat tgactttttg
    12601 acttttatct gttgcggaat gatttgtgat catcgttttt tgcaaaatct gtataaaaac
    12661 tatccaaata aaaagcaatt aacgactcgg ccgtttgccg ggatgatcga agaaatgatc
    12721 tatttttatc aattagaggc agaaaacctg tcaaattatg gtaaactgtt tgaacaagct
    12781 ttttatctga ccgaaaatgg ccatctactt tctgctagta tgaaaaaatt tttaggaatg
    12841 atgtttcatt tcgtgtggaa tctccaagca tatccttttg atttccaact aaaaaacttt
    12901 tttgtgtggc tggacttgct tggctatcca atccactggc tggaacagat ttcaaagatg
    12961 gagtatttgg aaaaagttct ttttttggca gaaaaaagca aaaaaatact tgttttagaa
    13021 aaaataggta taatagacgg ataccaacat ccaaacgaaa aagaattatt gagaacaatg
    13081 acggtatttc aacaaatcgt cagtgagata agagggaatt gaaatgaata caaatagcga
    13141 aaaaatgttg caagctttat cagatgaaga cttgtcacaa gcacaaatct ttttagaaaa
    13201 agcattaaaa gaagacccag cagatatatt agctgaacta ggcgaagaat tgctggctat
    13261 tggctttttg atcgaagcaa aacagatttt tgaacaactg atcaaccaat acccagaaaa
    13321 tgacggactg aacatcccgt tagcagaaat tgcaatcgaa gataatgaga tcgacttagc
    13381 ttttgaatac ctggaaaaag tggataagca aagtgattac tatccgcaaa gcttattggc
    13441 gatagctgat ttgtaccaag ttatcggaat ccctgaagtg agtgaagcaa aattgaaaga
    13501 agcagctggg ttgcttcctg atgaaccatt gatccagttt gcgctagctg aattgtattt
    13561 ttcagttgat cgtttcaaag aggcagcagt ggtttatgaa acattgttgg caagcgggat
    13621 aaatgaaatt tcaggaatct cgatgcagga acgtttagga caatccttaa gtatgcaagg
    13681 agaattcgaa gcagctatcg tacctttaga agaagcactg aaagaagaaa ggacagatga
    13741 tcgactgttc cagctagctt ttacgaatct tcaattgaaa gaaaatcagc aggcaatcaa
    13801 ctatctggaa gaacttcgag aagttaatcc gcattatcaa tcgttatatc tatatctagg
    13861 acaagctctt caagaagaag aaatgattga agaagcacaa acagtgttag aagaaggaat
    13921 caaagaaaat ccttaccaag tcgaacttta tcacctggcc tcggaaaatg ctttccgtct
    13981 gcatgataga cagcgagcag aagaactttt actgaaagct ttagaagtag gagacaaaca
    14041 agatgaaaca ttacttactt taagtaatct ttacttggat gatgaaagat atgaagatgt
    14101 gatacaagtg atcaatcaga tggaagaaac tgctaatcct tatgcagaat ggaacctagc
    14161 ccatgcttat aatgaattag aagactttac cgttgcagct gtacactatg aacaggctta
    14221 tcatgaacta tcacatgaac cagatttctt gaaagagtat gcctttttct taagagaaga
    14281 agggcagctg aaaagagcgc aagaattatt gacttattat ttaacacttg aaccaggaga
    14341 tatggaggca ctgtcattat tagatgacct aacagagagg tgagaaaaat gctaattgat
    14401 gtacgggaaa agaagaattt tttaacgtgg atggtcaatc atgtatcttt tagccgaaga
    14461 gaagtttttt ggatcttgaa ttatcttgcc aatcatgaag cgattttaag caatgtccac
    14521 tttgttgaag gggcgaattt tgcggatcga ggactgcaga tcagtgatct atctgttgaa
    14581 ggggaaccca tgaaattgtt tttgcaggga aaagaattta cggacacaga ccagattttt
    14641 catgaaatcc gtttgaattg gaaaaagcct ttatatgtgg aatgtatatt cgaaaatgct
    14701 tggcagacaa gagaatatct atctatctta gaagacaatc cattttcttc ttgggattct
    14761 tcagtcagtg aagaagatac tgaattaatt gaacgatatt ttcaagaaaa agaagaagag
    14821 gcaaaactaa aaatgcttta tgctcaaatc gatcaagcac tagaagatgg agatagggat
    14881 gcttttttga aattatctga tgaagttaac cgaaagatcc ttttgcagac acaaaagaaa
    14941 agtaaccggg taagtgaaca taacaattag ttaaaaatag tatagtacta aaaaagacaa
    15001 aggtcagctg gatgtggaat ctttcctcat agcctatctt tgtctttttt cttatagttt
    15061 tttcagtaat cttgtctgtg gcttcaaata agtaaagcct tgcccttcca cttcatgcac
    15121 attgatgatt gaaaggaaag ctttttcgtc aatctcgtgg acgatcctct tagcttcagt
    15181 taattcacta gagctaacga ccatatagat gatttttttt gaattaccag agaatgcgcc
    15241 ttctccagaa aggaaagtca cacctctttg cagaccggtc atcagtagtg gtgcaatctc
    15301 ttcgcttttg ttggagacga caagtagacc ttttgcagaa tatccgccat ctaagatcga
    15361 atcaatcaca cgactgaaaa catacgaaac aatcaacgta tacatcatac gtttcaagtc
    15421 gatataggtt aatgaagcaa gtaagacgat gatatcaaat gccaataggg aacgccccat
    15481 agtgatgccg taattttgtt ctaaaatccg agcgataata tcactgccgc ctgttgttcc
    15541 acccatgcgg tagacgatcc cgcttcctac acctgcaact aaacccgcaa gtagagaaac
    15601 gatcagtaga tcatgctgta aattgatttc tagtggaacc ctttgccaga tccataaaaa
    15661 gaatgacaaa gaaagtgtac ctagaacagt ataggccaag gaacgtttac cgaggatctt
    15721 tcctcctagt aaaatcaatg ggatgttgat taataaagtc gtataagctg gatcaatacc
    15781 aaataaagct cttaagatca aggtgatccc tgagactccc ccttctgcta aatgattagc
    15841 gatgttcaag taaacaagac caaatgcata aatacttgtt cctataatga taaaaagaac
    15901 ttcttttact gtttttcgtt tgaccatatg aattccagct ttcaattatg tattttcaca
    15961 gaatttccat ttagaaattc cttcctaaat gtagcatgga aattcgattt tctcaattgc
    16021 tcttatctag gatttgcgtt aaagttttgg taagatagtt ttggagggaa aaagatggat
    16081 aaaaagcgtt ctttatacag tatgcagcag gaagtggatg aatatatcca gcaattcaag
    16141 gtggggtatt tctctccatt agctcagatg gctcgattaa ctgaagaagt aggtgaattg
    16201 gcaagagaag tcaaccacac ttatggagaa aaaagcaaaa aggcttctga accagttaac
    16261 tcggtagcag aagaacttgg tgatgtttta ttcgttacga tgatcatggc taattcattg
    16321 aatatcgact taactgaagt ttttgaaaaa aacatggaaa aattcaatca aagagaccat
    16381 aatcgctttg agcgtaagga cgagtaaaca agatgaagtg gtcctggcct tgaagactct
    16441 gaaacggact tgtcaaacag tcaccatgca actgagtctt tatctcagga caagaaacac
    16501 gcaggaaagg ttggaaaaat gaaattaaaa caattacctc tggaatacca aaaggcaatt
    16561 ccagttctga aaaaattaga gaatgcagga tatgaagctt attttgtagg tggaagtgtt
    16621 cgtgatatct tgctgaacca gccaatccat gatgtggata tcgcaacaag cgcatttcca
    16681 gaagaaatca aacagctttt tccaaaaacg atagatattg ggatcgaaca tggaactgtg
    16741 ttggtattag aaaatgatga acaatatgag atcaccacat ttcggacgga atcaacttat
    16801 caggactacc gtagaccaga tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa
    16861 agaagagatt ttacgatcaa tgcgtttgct ttgaaagaag atggagaaat catcgatttg
    16921 ttcgatggtc tgcaagatct agaagaacag atcttgcgag ctgtaggcaa tcctcatgag
    16981 cggttccatg aagatgcctt acgaatgatg cgcgggttgc gttttgtcag tcaactaggg
    17041 ttcaaattag aggaagaaac cttctacgcc atcaaagaaa accacgagct attaggtaaa
    17101 atatctgtag aacgtataaa tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc
    17161 gcgggaatcc atgcatttat tgaaacagag tgctacattt gctgtcccgg tttgaaagaa
    17221 gaaggcgaag cacttttacg ttttgctgaa ttaccatccg tcccattgaa agaagagagc
    17281 caagcatggg cattattgat cagccaactg gatctaaaag aaaatgagat tcgttccttt
    17341 ttgaaatcgt ggaagtgttc taaccaaatg attaaagacg ttcaagcact tgtccaagga
    17401 ctacgacaac gagaaactgg agctttggaa ccattcgaat tgtacttctt aggcaaaaaa
    17461 catgcactgc aagtagaaga attgatgatc tattttgacg aaacacctca gatggatgcc
    17521 gtaggagaat cctatgatcg tttacctatc aagtcattaa gcgaattagc agtagatggt
    17581 aaaatcttgc tagaagaaac tggaaaaaaa cctggtaagt gggttagtga agctttgcaa
    17641 ttagctgaaa aagccgtcgt agaacaaaga ataaaaaatg ataaaaaaca ggttttggat
    17701 tttttatcga aagaaaaatt gatttaaaaa acactagact gtatctgctc agctttgttg
    17761 gctacttaaa gtgggcagat acgtcattag aagaaaaaac ataagatgtg accacttaca
    17821 taaatttgtg ctaaactgag gtagtacaag aataaattac atcataaagg agtcttttta
    17881 atgagtaaag aaatgacagc gctaaaattt tattttagaa atggagaaac ctggacgatc
    17941 aaccgccgtc atatcggcga tctttggatc aaacaaatca caacaagttt tggacgaatc
    18001 aatggcagtg aatttgttga aatccatccg tgtgctggat ttaaaatcga aatcttccat
    18061 gaaggggacg cagttgcgac acatgatatc aacctaggcg gactggaaat gggcatgttt
    18121 aatcgcgcat tgaaatacga agatatcgag cgaatggaaa tcctttaccg aaatggcaca
    18181 cctgatcttg tgtacttccc atatctagat aaaggaacag aaggattaga caatcaatat
    18241 caatcaacaa aaatcagcga aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa
    18301 cgtgtagaag atgtatacgg ggaatttttc gaataaatcg atttttacgc aaaaatgagg
    18361 ctgtgaaaga gatgtgtctt attactactg ataggctgtc tcttccacac cgtttattca
    18421 gaataaggga ctgtgacaag atttttgtcg cagccccatt tttttgttgt gataaaagaa
    18481 caagaaaggc aggaatggga aagtaaacat ccttttgtca gaaactttta tctttcttaa
    18541 gttttacttg atatctgata ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa
    18601 tttctaacta gcaataaacg atgaaacaat gaaatatttt tcatccaaaa agaactttgc
    18661 tataatgatg gaagtttgtg tataggagat ctggaaaatg agagagagtt ttgaacaaca
    18721 aaaaaaactg ttgcatgatc gctatggcgc cctttcgatg gatgaccgcc gtcaaatact
    18781 ttgtaagttg cgtaaaagaa atatattgat gtatcgtcag ttggaacgtc tgaaacatga
    18841 tctgcttcgt ttggagtcaa aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt
    18901 tgaagtagtc gaaaccaaaa ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca
    18961 aaataaaaaa taaaggggat ggacgatgga atttgctgag ttagttattg tatttgcagt
    19021 aacgatcacc ttatcgaaca tagctagccg cattttgcca atgattccag ctccactgat
    19081 ccaaattttc cttggagtgg tattaggtct gacagagtgg ggacaatcaa tcgattttga
    19141 acctgaattg ttcttagtta tgattattgc accattgtta tttagagaag gagaaaaagc
    19201 agatatttct tcgattctca aaaatttcgg gacaattttg ttcttggcat ttggaggagt
    19261 cattttgacc cttgtcgggg taggtgcgac gttatcattc ctgttgccaa gtgtcccact
    19321 tgctgcctgc tttgcttttg gagcagcgtt aggtccgaca gatgcggtag cagtcagttc
    19381 tctttctgga cgagtgaata tccctaaaaa ggcgatgcat attttggaag gagaaggtct
    19441 tttgaatgat gcatctggtg ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg
    19501 atctttttca gcagtaaacg cggggatgag tctagtagtt tctagtattg ggggagcttt
    19561 gatcggcttt ttgcttgtct ggttcaaaca aaaaataatt catttgatcg aaaaagcatc
    19621 agcacaagat gtcacagggt atctgctgat cgaacttttg cttccttttc tagcctatgt
    19681 attggcagag gcatttgatg tatctggaat cattgctgca gtggcagcag gtattctcca
    19741 agcttctggt tttaggaaaa tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag
    19801 tacatggaca actattgctt ttacactgaa tgcattagta tttatatttt taggaatcga
    19861 attgacgcaa gtgttctcgc cagtatgggg agacggttta tatccaaacg gtcttttatt
    19921 agcgattatc gtattaatct ctgtcatgct ttttgtgatc agattcattt ctatcagtct
    19981 tttttatgta ttcaaagatg gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat
    20041 cttaacattt ggcggtgtaa agggaacagt cagtttggct acgattttta ttttgccatt
    20101 ttcaatcaat aatatgatgt tttaccagcg gtctttgctt ttgtttctga cggcgggtgt
    20161 catcttagtg actttggtca tagggatcat tgtcttgcct atgctgacgg aaacagagga
    20221 agcgaaaagc acggatttga atgctttgat gattttagaa gaggtcgttg aagttttgcg
    20281 aaaagaaata aaggaaattg atcgtaatac aaaagaattt ttagccactg aagcagtgat
    20341 agaaaattat caagaacgga tacgtgatct gtacttggaa gatttaaccg atgatgaaaa
    20401 gcaagaagta caagaaatac aagcactgat tttgtcgatc gaacgggatg gattggatga
    20461 aagttatcgt tcgggcaagc tttcttctaa tggttatcgt ttttactccc gttttctttc
    20521 tcgattcgaa cattctatca caagccagat tttgtctttt atcggctttt ggttcattgt
    20581 tgtccgtagg ttaatgcgaa tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc
    20641 tgatcgtcag acatttatta gtgatgaaga tatccaagaa atcagagaga catatcaaaa
    20701 aaatactcag ttgattattg aaagtttaga taacttgact gatatttatg atgaaacgat
    20761 ggttcaattt tttatccagc aacgcaaact tgaaggaata aaaatgaact cggggaattt
    20821 gatttcttcg tggatgatcc agcaagatag tctgtttacg aaaaaaatgc taagaggtta
    20881 ttatcttgaa agaaaaatca ttgatgaata cgaagttgct gaaaaaatca cgacgttttc
    20941 tgcgaacagc tatcgcagga atatcaattt gttagaatct tatacgatga acaaaccatc
    21001 tgataacttt tctttccgat ttgcgtttcg agcaaagtca aaaaaaaacg agtccaataa
    21061 aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga
    21121 atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga
    21181 agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa
    21241 aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt
    21301 tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc
    21361 tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc
    21421 caactgatta ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg
    21481 ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg
    21541 aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta
    21601 aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta
    21661 ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc
    21721 agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag
    21781 acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac
    21841 aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa
    21901 accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt
    21961 acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc
    22021 ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc
    22081 agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag
    22141 aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca
    22201 aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc
    22261 aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta
    22321 atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc
    22381 atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt
    22441 acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag
    22501 aattacttga acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt
    22561 caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt
    22621 tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt
    22681 attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca
    22741 aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa
    22801 acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac
    22861 caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag
    22921 aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag
    22981 aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat
    23041 ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg
    23101 tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt
    23161 ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata
    23221 ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt
    23281 tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct
    23341 attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc
    23401 agactataat ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt
    23461 caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc
    23521 ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga
    23581 aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac
    23641 tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc
    23701 tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca
    23761 attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc
    23821 gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac
    23881 attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg
    23941 agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt
    24001 tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat
    24061 cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa
    24121 acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct
    24181 tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg
    24241 gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag
    24301 cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag
    24361 acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta
    24421 cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg
    24481 ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc
    24541 gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag
    24601 gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc
    24661 catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac
    24721 ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa
    24781 agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc
    24841 caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga
    24901 tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa
    24961 tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga
    25021 tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat
    25081 cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg
    25141 ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata tggagggagg
    25201 aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc
    25261 caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt
    25321 tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg
    25381 tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg
    25441 ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt
    25501 acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg
    25561 atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg
    25621 atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg
    25681 atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc
    25741 ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg
    25801 gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa
    25861 ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac
    25921 aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc
    25981 agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc
    26041 cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt
    26101 aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat
    26161 gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac
    26221 atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg
    26281 tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc
    26341 ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata
    26401 aagactacct ttttgataaa atttttgata acattagaac caatcgaata caagaggtga
    26461 aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat
    26521 catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt
    26581 aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga
    26641 ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc
    26701 aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa
    26761 tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa
    26821 tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat
    26881 ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca
    26941 ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta
    27001 cattttaggc atctacaatc cattttattt gaactttcct gagatcactg acatgcagac
    27061 aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta
    27121 ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga
    27181 agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt
    27241 gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc
    27301 agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata
    27361 aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa
    27421 ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt
    27481 atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga
    27541 gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc
    27601 cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca
    27661 ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt atattttgat
    27721 ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca
    27781 ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat
    27841 tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg
    27901 caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt
    27961 agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact
    28021 agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat
    28081 tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat
    28141 ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca
    28201 tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt
    28261 ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca
    28321 gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc
    28381 ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt
    28441 agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt
    28501 ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag
    28561 aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga
    28621 agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt
    28681 ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa
    28741 atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa
    28801 gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt gtactttatg
    28861 atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct
    28921 agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga
    28981 ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata
    29041 cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc
    29101 agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa
    29161 attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta
    29221 tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa
    29281 gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc
    29341 aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc
    29401 ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt
    29461 tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga
    29521 tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct
    29581 ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta
    29641 aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg
    29701 atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac
    29761 aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg
    29821 gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat
    29881 ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg
    29941 aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa
    30001 cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct
    30061 atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca
    30121 ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct
    30181 ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga
    30241 tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg
    30301 taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg
    30361 cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata
    30421 cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag
    30481 gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt
    30541 tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt gtttttttat
    30601 tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta
    30661 tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat tcttcatttt
    30721 ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca acaggaaaat
    30781 taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg tctatcatct
    30841 cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa ataaaaattt
    30901 gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc acttaactaa
    30961 tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa aaaacaagta
    31021 aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc caatggtttc
    31081 cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat gtggacatcg
    31141 tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg ctagatcaga
    31201 tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca gatccacaac
    31261 agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta gctatcacgg
    31321 cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca ttgaaagaaa
    31381 aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca atggtcttag
    31441 gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag aaaattgcac
    31501 agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta ggtaatgagt
    31561 tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa gaaatcggca
    31621 aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat gatttagcga
    31681 tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat cgttatggtt
    31741 tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt acagcaaaac
    31801 gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat attcgtcaag
    31861 gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa agtgatgact
    31921 gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga tgatcctaca
    31981 ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc ttcttttgaa
    32041 cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt ttgctttgaa
    32101 aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt aggaagagga
    32161 ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga gatcatatta
    32221 gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta tcaacaagtt
    32281 caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat cgatgatgtg
    32341 aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt gtccattgta
    32401 ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca ggaaaaaata
    32461 atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc taaagtatat
    32521 cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt ctcgcataat
    32581 gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat tacgccaatc
    32641 catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc cgcttttacg
    32701 tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag aagaaaattt
    32761 attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac ttgctaactg
    32821 gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga taggtaaaat
    32881 aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta actggggcaa
    32941 aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc ctcagctgct
    33001 tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt tactttcttg
    33061 taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa aaacagttta
    33121 ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct gcgcgaaagg
    33181 gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa tcgcagtcat
    33241 tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag aggtgattgc
    33301 aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa agcattgttt
    33361 tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct ttgaagccaa
    33421 gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa gaacagtttt
    33481 ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt tgatccaaga
    33541 cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag aataacacca
    33601 tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta gctacgattt
    33661 gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat tcagtaatgg
    33721 cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt gagaaatatc
    33781 ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta ccgaaaagta
    33841 aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata cgcggaaaag
    33901 gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt tacttggcaa
    33961 gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta cttgggttgg
    34021 atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca gtcaaagcag
    34081 cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa gctcgtcgta
    34141 ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag gtcaagaaag
    34201 ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac cgtgaaaaag
    34261 agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt aaaaaagctc
    34321 gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa ttaccagatg
    34381 cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa gttaaaaaag
    34441 tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt agcctgcaac
    34501 aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta gcacagcaat
    34561 tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca tacatgcgta
    34621 ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt attgaagata
    34681 cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct caaggcgcac
    34741 aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat gaactcaaac
    34801 aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt tttgtggcta
    34861 gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa aatggtgtca
    34921 tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg tatgtggaag
    34981 gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa gaaggcgatg
    35041 tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca gcacgtttta
    35101 gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct tccacttatg
    35161 caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa aaaagatttg
    35221 agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc cctcaaatcg
    35281 tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa gaaggcacgg
    35341 aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa ctgacgaatg
    35401 cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc gattgtgatg
    35461 tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat gcttgcagta
    35521 attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg acttgcccag
    35581 tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta ttctatggtt
    35641 gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc agaaattgtc
    35701 caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa gtagtctgtg
    35761 taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct ttcattgaaa
    35821 atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca gatcgcacaa
    35881 gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga ggcgcatcac
    35941 acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag tttggctaat
    36001 gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat cacttcggca
    36061 gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc tttttctgaa
    36121 acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga agaaattact
    36181 gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca tccattagca
    36241 gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc agctgcacca
    36301 atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg ctatgacaaa
    36361 ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc tttccatgag
    36421 gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa atattttgaa
    36481 ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct tttcggacct
    36541 atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc tgttatccag
    36601 ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca gactcacttg
    36661 aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa tgctgaattc
    36721 gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt gctgaaaccg
    36781 acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac tggtgtagaa
    36841 ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc acgcttagca
    36901 aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat ggcttactac
    36961 attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg tctgtttcct
    37021 gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc acaacgagct
    37081 ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc aaatgagtaa
    37141 ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg tttttcttaa
    37201 gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta gaaatattgg
    37261 ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat tctctgggac
    37321 aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg cgttatttga
    37381 ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac attctccatt
    37441 tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa tattttacgg
    37501 ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga aattctatca
    37561 gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa gaagtgataa
    37621 aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg ccacgttttt
    37681 tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt cctttggatt
    37741 tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta agtgagtgta
    37801 gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc catggtaaag
    37861 ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg caagaatatt
    37921 tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat atcgtttttg
    37981 tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg aatcaaatga
    38041 tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat acatttgcta
    38101 ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta ggacatgcca
    38161 atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa aaaaactatc
    38221 gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag gaaacaaaca
    38281 tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac gggaaatttg
    38341 ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa ggcactgcaa
    38401 agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga agtgttgcag
    38461 atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac gggaatctac
    38521 aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg caaaaacttg
    38581 aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt acaggagaag
    38641 ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt gcgttggctg
    38701 cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa attgcagaaa
    38761 atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt atcgtagaag
    38821 aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca aatcgtaagt
    38881 gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc cgttgctttg
    38941 cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat tacaccaaaa
    39001 aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg acgtttggca
    39061 aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga agtcggttat
    39121 gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat ccaaatcgta
    39181 gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga aaaacgtttg
    39241 gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa tcaatttgaa
    39301 caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca agaagaagtg
    39361 acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa gggtttactg
    39421 gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc aatgatgaac
    39481 aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt gacaccgaag
    39541 aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat caaggaagaa
    39601 tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt agcggaagaa
    39661 agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca gcaatctgga
    39721 gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg ttcacaagta
    39781 aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc aggagctttc
    39841 catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc gatacgggta
    39901 gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc aaataatgct
    39961 ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac gtttacaaaa
    40021 gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac agataatatc
    40081 ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt atttgaagca
    40141 ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga caaacttggc
    40201 tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg aggacgacaa
    40261 tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa aagaatactt
    40321 ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt ggtattcttg
    40381 acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat gaacgtcacg
    40441 atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct gaatcttata
    40501 ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact agtgatatta
    40561 ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact gtcgttccga
    40621 tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa caatcgtttg
    40681 cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg cagatccttt
    40741 atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc caaatggcaa
    40801 tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa gcattggatg
    40861 gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc actcgttctg
    40921 tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct cgttcgttag
    40981 gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag ttagcaaaac
    41041 acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag ccttcccatt
    41101 ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa aatgaccagc
    41161 tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca aaagaacatg
    41221 gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca ccggttttat
    41281 tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa acatatcaaa
    41341 tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat ggacaagaaa
    41401 tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca tttgattttg
    41461 aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat caagtggcaa
    41521 ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc aatgtacctc
    41581 ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg aaatcaagat
    41641 tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta ggtcagacaa
    41701 atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt tttgaaacaa
    41761 ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta actttaagct
    41821 ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg cctttcgttt
    41881 gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac ttgatggaaa
    41941 aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat tccatcacag
    42001 tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa ggtgtgtaaa
    42061 aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtcacactc ccgttttatc
    42121 tctttggaat gaaaaagtat caggattttt tcttgctgct attcaaacca aacgggattc
    42181 gactttcagt atggtttctg atacgcttga tattgtcttt atgccgataa aagataaagg
    42241 aagaaactaa aaaggcaatg atcgtcagta accaattggg cttagccaaa atcacaggag
    42301 ctacataagg aaggacgatc gtagataaag tgatcaatac tgcactgatc atactgctca
    42361 aactcaccat acttgtacaa aataaactaa cgataaagat gatacaagaa taaacaaaaa
    42421 atacaggatt aaatcctaaa agcattcctg cacttgtcgc aacagctttt cctcctttga
    42481 acccagcaaa aatcggaaat gtatggccaa gaatggcgca taccccaaac caaagagggt
    42541 taatcccaga aaggtggaag atcatcggca aacaagtagc caatgtacct tttaaaatat
    42601 ccataagcag gacaacaatc ccggctgttt tgcctaatac acgaaatgta ttggtcgttc
    42661 cagtattacc gctcccgtgt tgtcggatat cttttttgaa aaacaatttg ccaatccaga
    42721 caccggaagg aatagaacct aaaagatatg ctaaaataag taataagatc aatttcattt
    42781 tttaccctct ttcaagttga aaactatcct ttattttagc atgaataggt aacaggaaca
    42841 attgaaaaaa tgaaagatac aagtataata gatggaataa aaagaaaagc aaggggtggc
    42901 agaaatgtct gtaaaaaatc caacacaaga aacgattttc aactatttgg aagaagcaaa
    42961 aaatattgcc gtcgtaggct tgagcaataa accagaacga acaagttaca tggttgcaca
    43021 ggtattgcaa gacaatggat ataaaatcat accagtaaat ccattattgg ctggtgaaga
    43081 aattctaggc gagaaagtct atgcgaattt gacagaaatc gatgatccaa tcgatatcgt
    43141 agatgtattt agacggagtg aatttttacc tgaaatagca ggtgattttt tgaaaactga
    43201 tgctaaagtg ttctgggcac aattagggat tgaaaacaaa gaagcagctg agatgctaag
    43261 aaaagcagga cgtacagata tcgtaatgga tcgttgtatt aagattgaac ttggtaagtt
    43321 gaaagaaaac caaaaataaa aatgaccaca aagaagagat gatcgataga ggggaatgtc
    43381 gaaaaagcgg ttaggagata attaaaattg atttctcgtc taccctcttt tttttcacga
    43441 ccttttctag tatgataatc gtggtgcggt tttgccgcaa agtattattg aaggagcgtt
    43501 tgccttggca aaaaaagtaa acaacgaata caatgactcg tccatccaag tattggaagg
    43561 attagaggca gttagaaaaa gaccagggat gtacattgga tccactgaca gccgaggctt
    43621 gcatcattta gtctatgaaa tcgtcgataa tgcagttgac gaagcattat caggttatgg
    43681 cagtgagatt gatgtgacca tccatgaaga caatagtata actgttgcag atagcggacg
    43741 tgggatgcct gtaggaatgc atgcttccgg aataccaaca gttgaagtga tttttactgt
    43801 gctgcatgct ggcggaaagt ttggtcaagg agggtacaaa acttctggtg gactgcacgg
    43861 tgtaggagcc agtgtggtaa atgccttatc gaaatggcta attgtaacga tcgttagaga
    43921 tggcgtagag tatcagcaga aatttaaaaa cggcggtaaa cctgacggta ctttgaaaaa
    43981 gataggaaaa acaaaaaaag caaacgggac gactgtccat tttttaccag atgatacaat
    44041 attcagtaca acgaaatttt catatgaaat tttagccgaa cggttaagag aatctgcctt
    44101 tcttctaaaa ggcgttaaaa tctctctttc tgatcttcgt ggagaagaac cagtcaagga
    44161 aatttttcat tacgaagaag gaatcaaaga atttgttgat tatctgaatg aagaaaaaga
    44221 tacattgaca ccagttgttt atttttcagg agaaaaagaa ggtatcgaag tagaggtcgc
    44281 ttatcaatac aatgacggtt attcagaaaa tgttttatct tttgtcaata acgttcggac
    44341 aaaagatggg ggaacccatg aagctggtat gaaagcggcg atgacgaaat cttataatga
    44401 atatgcgaga aaagttggtt tgctgaaaga acgggacaag aatctggaag gtagtgattt
    44461 cagagaaggg ttagctgctg tactttctat ccgtgtacct gaaaatcttc tgcaatttga
    44521 agggcaaaca aaagaaaaac ttggtacgcc agttgctcgt accgtagtag ataacgtcat
    44581 cagtgaacaa atgggctttt atttgcagga aaatagcgaa atgagccaga tgttggtccg
    44641 taaagcaatc aaagccagag aagcgagaga agcagcacgt aaagccagag aagaaagccg
    44701 aaatgggaag aagcgtaaaa aaggcgaatc tcttctttca ggaaaattga cacccgcaca
    44761 atcccgaaac cctaagaaaa atgaattgta tttagtcgaa ggggattctg ctggcggatc
    44821 tgctaaacaa ggaagagaca gaaagttcca agcaattcta cctttacgag gaaaagtcat
    44881 caataccgaa aaagctaaaa tgcaagatat cttgaaaaat gaagaaatca atacgatgat
    44941 ctatacgatt ggtgcaggag tcggtcctga gttttctatc gaagactgta attacgacaa
    45001 agtaatcatc atgaccgatg cggatacaga cggcgcccat attcaagtat tactgcttac
    45061 tttcttttat cgttatatga aaccgctgat tgaagctggg aaagtatata tcgcgttacc
    45121 gcctttatat aaagtatcca aaggacaagg aaagaagcaa gtcattgaat atgcgtggac
    45181 agatgacgaa ttggcagcaa tgattaaaaa agtcggcaaa ggatatatgc tgcagcgata
    45241 taaaggactt ggagagatga atgcggaaca attgtgggaa acaacgatgg atccgacttc
    45301 ccgtacgttg atccgcgtcc gtatcgatga tgctgcgcaa gctgaacgtc gagtaaccac
    45361 actgatgggt gacaaagtag aacctcgaag aaaatggatc gaaaatcacg tgcaatttac
    45421 gctcgaagaa gatggcagta tcttagataa aaaagaggat actgaaatct ctccttcagt
    45481 ttctaatgat ttattagatg aagaacgagc agataaaaat gaaaacaatc agttatttga
    45541 agtagaatag gaggtcacct gatggaaaat cgacaagaag tacaagaatt aactttagaa
    45601 gaagtgatgg gtgaccgctt cggacgttat tccaaatata ttatccaaga acgtgcactg
    45661 cctgatatcc gtgatggatt gaagccagtg caacgccgga tattatttgc catgaacaaa
    45721 gatggcaata cgtatgacaa aggattccgt aaatcagcaa aatctgtcgg aaatatcatg
    45781 gggaattatc atcctcatgg tgacatcagt atttatgaag cgatggtccg catgagccaa
    45841 gactggaagt tgcgggaagt actgatcgaa atgcacggga ataacgggag tatggacggt
    45901 gatccacctg ctgcgatgcg ttatactgaa gccagattat ccaagttaag tggagagatg
    45961 ctagcagata tcgagaaaga aacagtcgat ctcgtgtgga attttgatga tacagaaaaa
    46021 gaaccaacag tccttccagc tcgataccca aaccttctcg tcaatggatc gacaggtata
    46081 tctgctggat atgcaacaga aatcccgact cataacttag ctgaagtgat cgatggcaca
    46141 atctatatga tcgatcatcc acaagcgagc cttgaaaaat tgatggaata tattcctgga
    46201 cctgactttc caacaggagg gatcttgcaa ggaaaagatg aaatcaaaaa agcttatgaa
    46261 acaggacgag gaaaagttat tcttcgttcg aaaacaaaga tagaggcaat gaagggtaat
    46321 aaacagcaaa tcgtgatcac agaaatccca tatgaagtaa ataaagcgac cttggtgaaa
    46381 aaaatggatg agatccgttt gaataagaaa atcgatggaa tagctgaagt acgagatgaa
    46441 agtgatagaa caggtctgca aattgtcgtc gaattgaaaa aagacgccaa tgctcaagga
    46501 attttgaatt atttgttcaa aaacactgag ctacagatca attacaattt caatatggtc
    46561 gctatcgacc atatgactcc gcatcaagtt ggactgaaag acattttaag aagttatatc
    46621 gagcatcgaa agcaagtcat aacaaaacgt agtcaattcg atttagccaa agctcaaaaa
    46681 agacagcata tcgtagaagg attgatgaaa gctttatcga ttctggatga agtaatcgca
    46741 acgattcgtg aaagtaaaga caaaaaagac gccaaaaaga atttagtcga cgtctttcaa
    46801 tttacagaag aacaagcaga agcaattgtc acattgcaat tataccgttt gacgaacacg
    46861 gatatcacag agcttcaaaa ggaatccgaa agcttgattg cgcaaatcac agagttgaat
    46921 aaaatccttt caaatgacaa agagcttttc tctgttatga aaaaagaatt gcgagaagtg
    46981 aaaaaaaatt attcttccgc tcgtctaacg acaatcgaag acgaaatcga agaaatcaaa
    47041 attgatacgc aagtcttagt ggcacaagaa gacgtaattg tcagtgtgac gcgagaaggc
    47101 tatgtgaagc gaacaagcct tcgttcttat agtgcttcca aaccagaaga aatcgggatg
    47161 cgagaaggcg actacttgct atactcaggt gaattgagta cattggacca tgttcttctg
    47221 atcacaaata aagcaaatgt gatctaccgc cctgtccatg aactacccga tttgaaatgg
    47281 aaagatgctg gtgagcacat ttctcaaaca atctccaatc tgtctgtaga cgaatcgatt
    47341 ttagctgttt tcccatatca aaaaattcaa gcagagaaaa catttgtatt catcagcaag
    47401 aatggattga tcaagcaaac acgaatgaca gagttcgaac catggcgcac ctataagagc
    47461 cgtccgctca gctgtatgaa gctaaaggcg gaagaagatg aactgattgc tgtgtatctg
    47521 aaagaagatc agccaggctt agatgttttc ttagtaacgc atcaaggaat gggtttgcgc
    47581 tatcctttaa cagatgttcc cgttgttgga gcaaaggcag ctggagtgaa gtctatgaat
    47641 ttgaaagatg gagataggat tatcaacggg ctgcttgtgt tagcagaagg tgatacgccg
    47701 atcgtaatcg ttactcagcg aggagctgtt aaacgcatgc ttgctcaaga aatcagccaa
    47761 acttcacgtg cgaagcgggg agtgacggtc atgcgtgagc tgaaaaaaaa tccgcaccgt
    47821 attatctata tgtcggaagg aagatcaaaa gaaataacct tgatgaatca aaaagggcag
    47881 cagttaacga ttgacccaac tgatttccct ataggtgatc gaacatcgaa tggatcattt
    47941 gcgatggatg agaaaaaagg tggagaagtc atcgaagtta tcgatgctcc tgagattttc
    48001 atcgagtcgt aactgttgta gtattttaat gaagagacgc catctgtatt aatacaggtg
    48061 gcgtctcttt ttcattcatt tttaataaat ccttatacgt ttgcaaaaaa agatgaaagt
    48121 gttcacaatg cttaaatgtt ggtatgtcaa cgttgattta aatatatgta aataaaacat
    48181 aaaaattgtt aaataaaaaa caagaaaacc cttgcaaaga tagggtgctg tgttatacta
    48241 atcacgtaaa aactgttaca taaaacagaa atttaggagg tccatacaat ggagcaatgg
    48301 aatggattta aaggtaccaa atggaaagaa ggcgtagaca ctagagactt tattcaaaat
    48361 aactacactg aatatcgcgg tgatgacagc tttttggaag gcccaacaga agcaacagaa
    48421 aaattatggg atgaattgca aaagttaaat gatatccaat tcgagcgtaa cggtgtctat
    48481 gatatggaca cagaaatcgt atcaactatt acttcacacg atgcaggcta tttggataaa
    48541 gatttggaaa aaatcgtcgg tctacagaca gacaaacctt tgaaacaagc attcatgccg
    48601 ttcggcggaa tcaatatggc taacaatgcg ctaaaaagca atggctatac accaaacgat
    48661 gaattgacac atattttcac tgactggcgc aaaacacata atcaaggtgt atttgacgct
    48721 tatactgctg aaatgcgtgc tgctcgtaaa aacaaaatca ttacagggct tccagacgct
    48781 tacggtcgcg gacgtatcat cggtgactat cgtcgtgtgg cattgtatgg aatcgactat
    48841 ttgatggaac aaaagaaaaa agatcatgat atgactggtt atcatacaat gagtgaagaa
    48901 gtgattcgtt tacgtgaaga aatcactgaa caatatcgtg cattagcgca aatgaaagaa
    48961 atggctgcga aatatggcta tgatatttct aaaccagctg caaatgcaaa agaagctgtc
    49021 caatggctat acttcggata tttagcagca atcaaatcac aaaatggtgc tgcgatgtca
    49081 atcggacgta tctcagcatt ccttgatatc tatatccaac gtgatttgga taatggagta
    49141 atcacagaaa aagaagcaca agaattgatc gaccacttag taatgaaatt acgtatggtc
    49201 aaattcgcgc gtacacctga atataaccaa ttattctcag gattccctat ctgggctact
    49261 ttatccattg caggtatggg attagatggt cgttcattag ttacaaagaa tgatttccgt
    49321 ctgcttcata cattaacaaa tatgggacct tcaccagaac caaacttgac tgttttgtat
    49381 tctgaacacc ttccagaagg attcagaaca tatgctagca gaatcgctat cgaaagttct
    49441 tcgattcaat tcgaaaatga taatcagctt cgtgaacaat ggggttctga tgattgtgcg
    49501 attgcttgct gtgtatcagg aacagttgtc ggaaaagaca tgcaattctt tggcgctcgt
    49561 gctaacttgg ctaaaacact attatatgcc atcaatggcg gagtcgatga agtgactggc
    49621 gcccaagttg gaccagaata tcgtccaatc acatcagaaa tactagactt tgaagaagtg
    49681 aaacataact tcatgaatat gatggattgg ttagcagaac tatatgtcaa tacgttgaac
    49741 atcattcatt atatgcatga taaatattct tatgaagcag ctcagctggc tttgatggat
    49801 tctgacttgc aacgcacatt tgctacaggt attgctggga tctctcatgc ggcagacagc
    49861 ttgtcagcga tcaaatatgc aaaagtaaaa gcaatccgta atgaaaaagg aatagcagtt
    49921 gattttgaag tggatggcga cttccctaaa tacggaaatg atgatgaccg tgcagatgaa
    49981 ttagtttcat ggatcttgga atacttcatg ggacaaatca aacgtcataa aacttaccgc
    50041 aactcaaaac caacaacttc attattgacg attacttcaa atgttgttta cggtaaaaat
    50101 acaggaaata ctcctgatgg ccgtgaagct ggaaaaccac ttgcgccagg agcaaatcca
    50161 agttacaatg cggaacaaag tgggttatta gctagcctga actcaacagc taaaatgaat
    50221 tacggctgtg cattggatgg aatttcaaat acccaaacaa tcaatccttc aggtcttgga
    50281 aaagaacaag atacaagaat cgaaaacttg cgtaatgtct tagatggata cttctcaaaa
    50341 ggagcttatc atttgaatgt caatgtattc agtaatgatt tattgcgtga tgcaatggaa
    50401 aatccaatga aatatccgaa cttgacgatc cgggtatctg gttatgcagt taaattccgt
    50461 gacttgacaa gagaacaaca attagatgtc ttgatgagaa cttcacacga cagaatgtaa
    50521 aaagttcgtg aaaaaagttt ttcgacttag cgtcgaaagg acgttgaata gccatcgcat
    50581 caaaatcgaa taatcagtat gcttatttga aagaaacgat ttacaggggc aggacttgta
    50641 tgttccgtcc ctgtaaattt tacacataac gaagcggcaa ttgcgaaaaa ttttgttcag
    50701 aaagcttcgc ttcgacagct aacttgtact taaaatgaaa tcaaagaaaa gatgtgaact
    50761 taaatggaag aaaaaacaat cggctatgtt cattctatcg aaactttcgg ttcagtagac
    50821 ggacctggac tgcgctttgt cgtgtttatg caaggatgcc gcatgcgttg ccaattttgt
    50881 cacaatccag atacttggaa tattggcgga gggaaagaat acacagcaga tgaactttta
    50941 gataaagcag aacgcttcag accttactgg ggagataaag gtggtattac tgttagtggt
    51001 ggagaacctc tattgcagat cgattttttg atcgaactat ttaaaaaagc aaaagagcgg
    51061 aagatgcata ctacattgga tacatgcgga aaacctttta cgtatgaaga acctttcttt
    51121 tcacgttttc aagaattgat gaagtataca gatttactgt tatttgacat caaacacatc
    51181 gataatgaag agcataaaaa actaacgcat tgggacaatg aaaatatttt agaaatggct
    51241 caatatttgt caaaaatcaa caaaccggtt tggattcgcc atgtactagt tcctgagcga
    51301 agtgattatg atgaatattt gatccgacta gacaacttta ttcaaacctt gtcaaatgta
    51361 gacagagtag agattcttcc ttatcatacg atgggaaaat acaagtggga aacacttggt
    51421 ttgaaatatc cattagaagg gatcgaacca cctacaaaag aacgtgtcga aaatgctaaa
    51481 cgattgcttc atacagaaga gtatacaaaa tatttaacgc gataggtcgc atcggaaaga
    51541 agataagtat tgcttctttc cgtttttttg ctattattaa ataggagaaa tcacaaagga
    51601 gcgaatgaca tgtcaaaaat tttagttttt ggtcaccaaa atccagatac agatgcaatc
    51661 ggggcagcca ttgcgttttc acacttgcaa aatgaattag gtaaagaaac agaagcagtt
    51721 gctctaggta cacctaatga agaaacacaa tacgcattag accactttca gctatctgca
    51781 ccacgagtag tagagtcagc taaagcagaa aatgcagatg tcatgcttgt ggatcataat
    51841 gaattccaac aaagtatttc agatatcagc gaagtaaata ttttagctgt agtagaccac
    51901 catcgtattg caaactttga aacagctaat ccattgtact atcgtgcaga accagttgga
    51961 tgtacaagta cgattatttt aaaactatat aaagaaaacc aagtaacaat tcctaaagaa
    52021 atcgcaggaa tcatgctctc agctatcatt tcagatacat tgttgttcaa atcaccaaca
    52081 tgtacgcctg aagatgaagc agctgcaaaa gaacttgcag caatcgctga agtcgatcta
    52141 aacacttatg gacttgaaat gttgaaagca ggaacaaatc taagtgataa atcagctgcg
    52201 gttcttcttg atttggatgc aaaaagtttc ccaatgggca gttccaacgt tcgtatcgct
    52261 caagtcaata cagtggacct taatgaagtg atggatcgac aagcagagct agaagcagca
    52321 atggaagatg aaatgaaagc aaatggctac gatctgttcg tattagttgt cacaaacatc
    52381 ttagacagtg attcagaatt acttgttgct ggtaaaggaa aagaaaaagt agaagaagct
    52441 ttccaaacaa ctttgaccaa caaccgtgct ttgcttaaag gtgtcgtttc acgtaaaaaa
    52501 caagtcgtac ctcaattgac ggctgtattt agctaatcaa atggtagtat gaagcattca
    52561 aaatgaatgg ataagcgatt gatataaaaa gaaacataga acatactaaa ggccgaagaa
    52621 atggaggaga ataacagtca attatgactc ttgttcttcc tcctttttac gtaaaagcct
    52681 tttaagtaaa ataaaacgga tagaaaagag aacatatatg aactattatt caaaaaataa
    52741 ggaaaacttt tatcaaaaac tgaccgatga tccgcttttt tcttcattga ccgattatct
    52801 atatgaacat cgagaacaag aaacgattct aagagaattg aaaaaagaat tttcgcaaaa
    52861 taaattttcc cattttcttg atttattgat cgatgcagga ttaatcaaga gggaagaacg
    52921 aagatatcat ttgaactttc caatatttga tcctaaagat tacttgcaac aagccacttc
    52981 ggctgctgaa accatagctg aacaattgaa gagactctct gttgatgaac aaaaattagc
    53041 gatgggagaa gttatttggg cgtattgttt cgaagatgaa cggaaagaag cgtattttta
    53101 tggagtaaga aacagccgag agacagaatt gctgaggaca actgcaggaa atgagaagta
    53161 ccgttttatc acactttcct ctatagaaca cttcccgctt actttggcta attacttttt
    53221 tgtacagaag aatcagcttc ctgtaacgaa ggcattcaaa gaattagccg aacttattgg
    53281 agacgtaaat gaatcgtact tttttgatca aatagaagtt atcgttgaca ggatacgtaa
    53341 gaataaatat aaaaatcgcc ggcctagtat tttccatcag tctctacttg tgacgaatac
    53401 tataaaggaa gaagaatcat tcacattaga attacctatt gtcgaaaaaa acaattttga
    53461 gattgaatta ccaacattgg atccttcatt gacaatggag gaaatggcct ttcttaaacg
    53521 acaaatcttt tctgagctga gcaagaaatt tataccgcat gcattttctt atatcaaaga
    53581 atatggaaca gttttagtga gtaaaaccta agagagcagt aagcaaaaat tatactgact
    53641 cttgcaggag gagcctttct cttcctttat ggttaaaaga attatttgag tattttgcct
    53701 atctaccacc tattagattc actacatggt ctggaaacaa aggaatgcaa aaaaatggtg
    53761 agaaatgtgg aatgacaaag gaagtagtga taagaaaagt tagattttta aataatcaat
    53821 attatgattc agtcaagtat ggcattttat gggaagaact agctgattag attaggaagt
    53881 ccatttgata atccagaatc actaagaaac gaatggcgag tcattaataa tttatccaat
    53941 cttgggttat tcttttagat tgaagtgttt aaaactaaaa aagcatttat gaagtcgtaa
    54001 tagtcaggct tcaaaatgct ttttattttg tttgtttttt ctttgccaca taaatacacc
    54061 tagactgacg atgaagagca tatccaacag atttcctgcg acttggatcg tcgaagggtt
    54121 ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa aacaggtgac
    54181 aggaactaat cgaaagaaga gcggacattt caattggcta aaaatgagtg aaaaaataaa
    54241 ataagtaaaa atggataaaa tcacgactaa accattcatg ataaagaaga aaagcgtatt
    54301 gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg caatcaatac
    54361 gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa aaataggcac cgaaataaac
    54421 agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt cttcggcttc
    54481 tgtgatatca gaatgatcca aaagcaacca gcctaatgca ctagaaggcg catgtgaggt
    54541 cttaaggtat gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa aagagcctaa
    54601 atgtaaaaaa ttagtggttc tacgttgaat ttccataat
//
LOCUS       DAYYKC010000008        52830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000008 DAYYKC010000000
VERSION     DAYYKC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.008"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..679)
                     /locus_tag="ACHBBW_000496"
     CDS             complement(101..679)
                     /locus_tag="ACHBBW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152208.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(699..1367)
                     /locus_tag="ACHBBW_000497"
     CDS             complement(699..1367)
                     /locus_tag="ACHBBW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152209.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1382..2452)
                     /locus_tag="ACHBBW_000498"
     CDS             complement(1382..2452)
                     /locus_tag="ACHBBW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152210.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2623..3987)
                     /locus_tag="ACHBBW_000499"
     CDS             complement(2623..3987)
                     /locus_tag="ACHBBW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9152211.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3984..5543)
                     /locus_tag="ACHBBW_000500"
     CDS             complement(3984..5543)
                     /locus_tag="ACHBBW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152212.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5599..8079)
                     /locus_tag="ACHBBW_000501"
     CDS             complement(5599..8079)
                     /locus_tag="ACHBBW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152213.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8063..8398)
                     /locus_tag="ACHBBW_000502"
     CDS             complement(8063..8398)
                     /locus_tag="ACHBBW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152214.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8412..9446)
                     /locus_tag="ACHBBW_000503"
     CDS             complement(8412..9446)
                     /locus_tag="ACHBBW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9152215.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9536..10216)
                     /locus_tag="ACHBBW_000504"
     CDS             complement(9536..10216)
                     /locus_tag="ACHBBW_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9152216.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10500..11603)
                     /locus_tag="ACHBBW_000505"
     CDS             complement(10500..11603)
                     /locus_tag="ACHBBW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9152217.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(11825..12295)
                     /locus_tag="ACHBBW_000506"
     CDS             complement(11825..12295)
                     /locus_tag="ACHBBW_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9152218.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            12628..14268
                     /locus_tag="ACHBBW_000507"
     CDS             12628..14268
                     /locus_tag="ACHBBW_000507"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9152219.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14422..15744
                     /locus_tag="ACHBBW_000508"
     CDS             14422..15744
                     /locus_tag="ACHBBW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9152220.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(15806..16237)
                     /locus_tag="ACHBBW_000509"
     CDS             complement(15806..16237)
                     /locus_tag="ACHBBW_000509"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152221.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(16301..17137)
                     /locus_tag="ACHBBW_000510"
     CDS             complement(16301..17137)
                     /locus_tag="ACHBBW_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM9152222.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(17879..18505)
                     /locus_tag="ACHBBW_000511"
     CDS             complement(17879..18505)
                     /locus_tag="ACHBBW_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152223.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(18800..19135)
                     /locus_tag="ACHBBW_000512"
     CDS             complement(18800..19135)
                     /locus_tag="ACHBBW_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9152224.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19122..19406)
                     /locus_tag="ACHBBW_000513"
     CDS             complement(19122..19406)
                     /locus_tag="ACHBBW_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9152225.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19462..20985)
                     /locus_tag="ACHBBW_000514"
     CDS             complement(19462..20985)
                     /locus_tag="ACHBBW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9152226.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(20978..22153)
                     /locus_tag="ACHBBW_000515"
     CDS             complement(20978..22153)
                     /locus_tag="ACHBBW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9152227.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22157..22342)
                     /locus_tag="ACHBBW_000516"
     CDS             complement(22157..22342)
                     /locus_tag="ACHBBW_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152228.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22308..24002)
                     /locus_tag="ACHBBW_000517"
     CDS             complement(22308..24002)
                     /locus_tag="ACHBBW_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9152229.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(23999..24472)
                     /locus_tag="ACHBBW_000518"
     CDS             complement(23999..24472)
                     /locus_tag="ACHBBW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9152230.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(24541..24696)
                     /locus_tag="ACHBBW_000519"
     CDS             complement(24541..24696)
                     /locus_tag="ACHBBW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9152231.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(24830..25210)
                     /locus_tag="ACHBBW_000520"
     CDS             complement(24830..25210)
                     /locus_tag="ACHBBW_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9152232.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25214..25429)
                     /locus_tag="ACHBBW_000521"
     CDS             complement(25214..25429)
                     /locus_tag="ACHBBW_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152233.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25432..25839)
                     /locus_tag="ACHBBW_000522"
     CDS             complement(25432..25839)
                     /locus_tag="ACHBBW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9152234.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26105..27580)
                     /locus_tag="ACHBBW_000523"
     CDS             complement(26105..27580)
                     /locus_tag="ACHBBW_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9152235.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(27570..28418)
                     /locus_tag="ACHBBW_000524"
     CDS             complement(27570..28418)
                     /locus_tag="ACHBBW_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9152236.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28455..28817)
                     /locus_tag="ACHBBW_000525"
     CDS             complement(28455..28817)
                     /locus_tag="ACHBBW_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152237.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(28818..28976)
                     /locus_tag="ACHBBW_000526"
     CDS             complement(28818..28976)
                     /locus_tag="ACHBBW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152238.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29117..29741)
                     /locus_tag="ACHBBW_000527"
                     /pseudo
     CDS             complement(29117..29741)
                     /locus_tag="ACHBBW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(29738..30049)
                     /locus_tag="ACHBBW_000528"
     CDS             complement(29738..30049)
                     /locus_tag="ACHBBW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152239.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30093..30386)
                     /locus_tag="ACHBBW_000529"
     CDS             complement(30093..30386)
                     /locus_tag="ACHBBW_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152240.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            30580..31227
                     /locus_tag="ACHBBW_000530"
     CDS             30580..31227
                     /locus_tag="ACHBBW_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9152241.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31288..32433
                     /locus_tag="ACHBBW_000531"
     CDS             31288..32433
                     /locus_tag="ACHBBW_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9152242.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(32514..32819)
                     /locus_tag="ACHBBW_000532"
     CDS             complement(32514..32819)
                     /locus_tag="ACHBBW_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9152243.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(32844..33275)
                     /gene="ruvX"
                     /locus_tag="ACHBBW_000533"
     CDS             complement(32844..33275)
                     /gene="ruvX"
                     /locus_tag="ACHBBW_000533"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9152244.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(33272..33538)
                     /locus_tag="ACHBBW_000534"
     CDS             complement(33272..33538)
                     /locus_tag="ACHBBW_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9152245.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(33793..34518)
                     /locus_tag="ACHBBW_000535"
     CDS             complement(33793..34518)
                     /locus_tag="ACHBBW_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9152246.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(34879..34949)
                     /locus_tag="ACHBBW_000536"
     tRNA            complement(34879..34949)
                     /locus_tag="ACHBBW_000536"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34915..34917),aa:Gly,seq:ccc)
     gene            35066..35818
                     /locus_tag="ACHBBW_000537"
     CDS             35066..35818
                     /locus_tag="ACHBBW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9152247.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            35834..36313
                     /locus_tag="ACHBBW_000538"
     CDS             35834..36313
                     /locus_tag="ACHBBW_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9152248.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(36564..38144)
                     /locus_tag="ACHBBW_000539"
     CDS             complement(36564..38144)
                     /locus_tag="ACHBBW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9152249.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(38137..38880)
                     /locus_tag="ACHBBW_000540"
     CDS             complement(38137..38880)
                     /locus_tag="ACHBBW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152250.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(39056..39832)
                     /locus_tag="ACHBBW_000541"
     CDS             complement(39056..39832)
                     /locus_tag="ACHBBW_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9152251.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(40080..40916)
                     /locus_tag="ACHBBW_000542"
     CDS             complement(40080..40916)
                     /locus_tag="ACHBBW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9152252.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(40927..41856)
                     /locus_tag="ACHBBW_000543"
     CDS             complement(40927..41856)
                     /locus_tag="ACHBBW_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9152253.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            42048..42401
                     /locus_tag="ACHBBW_000544"
     CDS             42048..42401
                     /locus_tag="ACHBBW_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9152254.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(42475..43131)
                     /locus_tag="ACHBBW_000545"
     CDS             complement(42475..43131)
                     /locus_tag="ACHBBW_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9152255.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            43357..45291
                     /locus_tag="ACHBBW_000546"
     CDS             43357..45291
                     /locus_tag="ACHBBW_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9152256.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(45377..46600)
                     /locus_tag="ACHBBW_000547"
     CDS             complement(45377..46600)
                     /locus_tag="ACHBBW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9152257.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(46594..48465)
                     /locus_tag="ACHBBW_000548"
     CDS             complement(46594..48465)
                     /locus_tag="ACHBBW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9152258.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(48486..49604)
                     /locus_tag="ACHBBW_000549"
     CDS             complement(48486..49604)
                     /locus_tag="ACHBBW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9152259.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(49687..50520)
                     /locus_tag="ACHBBW_000550"
     CDS             complement(49687..50520)
                     /locus_tag="ACHBBW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9152260.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(50513..51430)
                     /locus_tag="ACHBBW_000551"
     CDS             complement(50513..51430)
                     /locus_tag="ACHBBW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9152261.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(51453..51632)
                     /locus_tag="ACHBBW_000552"
     CDS             complement(51453..51632)
                     /locus_tag="ACHBBW_000552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152262.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(51657..52781)
                     /locus_tag="ACHBBW_000553"
     CDS             complement(51657..52781)
                     /locus_tag="ACHBBW_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9152263.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15697 a        10709 c         8877 g        17547 t
ORIGIN      
        1 tttgtattta tgaaaaacta cctatataaa gaagcaagat ataacgatct tcgtcatatc
       61 ttgcttcttc acctaactat tttctcccta tataacagct ttacgcttct cctaatacac
      121 cattcaaata taaatcgatc aattgcgcca catttagcgg ttcttccaca ggtcttttcg
      181 accgagtgat gtaaggtgct aacactaaga aaaaatgcct tgctgcgatt tcttttgaaa
      241 tcccatttct tagcacagat tggtttgctg aaaagatttc ttcaaaaggt tcacgataat
      301 ttttcttcca tagaaagaaa agatgatctc ttgtttcctt tgaaagattg ctttcaagat
      361 cgtgaagcat cagaaacaaa tcgaccagat gtgtctcaat caaaaactgc gccatttttg
      421 tcaaggttac ttgaaatccc gtttcgcttg tttgaacgat ttctctcaaa gcttttccaa
      481 catcttttaa gtggttttct aaaactgccg tatatagttt ttctttatca ccgaaataat
      541 gatagagatt gggctgggtg atcccaactt tttgtgcgat ttctcttgtt gaagtgccga
      601 gatatccctt ggacataaac aagtcagtcg cttctttcaa aattttttgc tgtgtatctt
      661 tgttgtatcg atggaccatt tttccacctg ctttttggtt attctttttc tttcaataca
      721 ccatcttcca tcataaatat tctatcgcat ttgtcaatca gccgtgtatc gtgagtaacc
      781 atgatcgttg ctttattttt gtcttttgat tcttttgcca agatatcgac tacttctcgt
      841 gccttttctg aatccaaact agcagttggt tcatctgcta gaataatgga cggatcattg
      901 tacaatgcac gagcgattgc gaccctttgc cgttctccac cagatagttc ttctggatac
      961 ttgttgatca acttatccac gccaagctgc tcaaataatt cgtttgcttc tttttgacga
     1021 tgttcatgtt taatcttatc tactaataat aactgctttt ttactgtcag aaaaggaacc
     1081 agattggaag cttgcaaaat aaagccaatt tctttaaaac gtagtttaac gcgttcgctt
     1141 tctttttgat tactgaattc ttgatcattg ataaatacag agccactgct aggcgtctgc
     1201 aatccgcctg ccaaagtcaa aaaagtactt ttacctgaac cagaaggacc gatgattgca
     1261 acaaattccc ctttatcgat actcatggtt gtttctttca atgctttaac tacttggtcg
     1321 ccatcttgga aacttttttc tacttcttta aattctattg ctttcatgtt cttcctcctt
     1381 cttatccaat tgccttcaac ggatcgattt ttacgattgt ccgaacagaa aatagtgcgc
     1441 ctaacacagc tactactaac atcaacaagc taatgacgcc aaaaaacagc caattgcttt
     1501 ggaatggaac cgcatcaggt agaacaaggg atgttcccac tgttcctaat agaccaagaa
     1561 gaataccaac aaatgataaa atgaatgttt gtgccactac agaacgcgca ataaatcctc
     1621 ctgtgatccc ttgtgctttc attacaccaa agatattaat cttttgcatc gtcagtacat
     1681 aaatgaaaat cccgatcaca atagccgcaa taacgatcaa aaatccgatc ataaatccaa
     1741 aagtcaatac ttgcgcatta tatccaggta attcattgat gaatttagaa attttgatct
     1801 gttctaaatc ttcaggtaaa ttactgattt tacctctgac aactattgcg ttaatccgtg
     1861 cattttcact tgtatcttct ttttcgaagc ggatttcttg gtaagcattg atggttgtat
     1921 agagaactgg tgagacattg aatttggcat gatccgtaaa gccagttagt ttaaacgtct
     1981 tgtcactacc agataactta actgtatctc ctatggctag accatattct tctttcaaac
     2041 taatatcgcc tactgcctca tcatcgttat caaaagcttt tccttcaaca agattgggca
     2101 tgatgaattg atttttatcg ataccgaaga agctaacatt gatttttcct tcttctgtac
     2161 tgtctttact agtcacgact ccaggtgttt gggcaagata cgccactttc ccaccttcga
     2221 cttcttctgc tatcttctta gtaatcatgg acatccctag atttgaattt gcatctttcg
     2281 aaagcacaat cgaatctgcc tcccacttgt cgactgctgt tcgattatct tgagctaagc
     2341 cataagctaa cccagtcaaa aaaaatacca ggtaggcgat caaaaacatc acccctgcaa
     2401 ctaacgcata acgtaacttt gaatgcataa tttcatttaa tgctaaaaac atttaactcc
     2461 tcctcctgaa ataatcattt tatacaacaa ttttatcact tgataaattt ttggcgaata
     2521 ttatgctcaa aaaattcttc cgctatttca ttatatgaat tttttattct cgattgatta
     2581 atatttatta tatccccaca caataaaaga ccgtgatatt tctcacagtc ttttatctac
     2641 tctattgcta tttgctctct tagctagtac tttaaaagct aactcattga ggtagtgaaa
     2701 acgagaggtg tattcagaca gaaatccaaa atcctcaact gctagctgtg gaatcgtagt
     2761 tttgtgtttc acgatatccg caagcaggtt caaatctgat aataagaatt cttggcttat
     2821 cagctgtccg ccaattattt ctaatgtatc cggatggtag atcagctttg catttactgt
     2881 tttgttttct tcgtcgtatt gtggaaatag atcaaattct cctgaacacg aaccggtatt
     2941 ttgttccaag aatggcgctt catcttctgt cagccctaca gttcctaaaa agtagtctat
     3001 aatgactgct gctgttatct tttgtctgag gtcgtaagcg gttggctgac cactcaaagt
     3061 tagcgctact gctcttgcca tatgtacagc tcgagtaact aaaggtaaaa agagatgccg
     3121 tttatttttg tcaaaactga ctgggacgat atctccaatc gcatagatgt ccggatcact
     3181 tgtctgcaga tattcattga ctatcacagt ctcatcctca tacaaagcca ctttttcttt
     3241 taacaatgtc gtattaggac gagtttgggc agaaaaaatg accatatctg cctttattgt
     3301 ttgagacaga agttgtacgg atactacttt ctcttctgat tttttccaat cgatcaagaa
     3361 ttcatttgga taaaaattta tttgcttagt ttgcagctga ttttgaaaac tgttgatcat
     3421 atccttatcg aaatatcttg ataaaacatt cggcatataa tcaactagat gaataatgta
     3481 tcctttattt ctcaaagcat ctgctaattc caatccaatg taaccagcac cgaagatcac
     3541 tacttctttt gcttctttta aagcggcaag tacttgctta gattgcacta gatttttata
     3601 ggtgtagaca ttttcagctt caatttgttt atacaaggtg acaggagagg aacctgtcgc
     3661 tacgattaat ttatcatagg ttatggtctc gttttttcct tctttggatg cgaggatgag
     3721 ctgatgtttg tctggatcaa gttcagttac tctccattcg gtgatcagtt ttgccccact
     3781 ttcttgaaga gtccgcatgt tatgggcaac ttcagctaaa ctacgtatct ttccttttaa
     3841 ataaaggttg attccatttg aaacatagcc tagtccatct ttatgatctt catcgatcag
     3901 taaaacttct gctttcggtt gaagttttct tagatttagg gcagctgtga tgccttcatg
     3961 ggaagcacca atgatcacga ttttcatgtt ttattcatcc ttaaattcaa tttttctaaa
     4021 taacattctt ttaaaaattg gttcaaatag gggaaatcat aattatattc gttgctagtg
     4081 aatacaaaaa ctttagcgga cttctgtgac gggtactctt tcaattggct agtcaaaatg
     4141 atatcatact gcttttcttt ctcgtattcg actaaatcat atttgatcgt attcagcaat
     4201 tgttcctgga tgatttcttt cacgatatcc gccattagcc tttcataagg aagatcataa
     4261 gcaatttgca gacgtaattc taactgcttg atcagcgtcc gtaaaataga tgcatagcga
     4321 gcaaataatt caaagcgggc attttcctcg tctacttcag taaaatcaac aatagcaaat
     4381 gtttgtctga ccaatctttc acataaaacc aggaattgtt tatcttctac ttcagttaac
     4441 cgctgtttta atccttcttg gccaaaaata ttcatccatc ctctaaaata ggcaaaatga
     4501 gcgtgatttt gcatcactgt caacgcaatc ttttgcgaaa tctcttctgg aaaaacataa
     4561 aaagagaaat attcttctat cgattgttta aacgttcgat tcaattgagc tactacgtcg
     4621 tctttgtatt tttcgcttat caaaaactcc tggacatgcg agttatgtgt atctaatgta
     4681 aagttactga tcatgaaaag ataaaaaacc aacgtttcat tttgattcca aggaaaggca
     4741 tattgaaaga agtaacgggt caaaaatgta tggaggtcca ataaaaattg tgtcgtttga
     4801 aacaatccaa caggaagatt gctataatct ggtttggatt ttttcgtaca gcgcattcgc
     4861 ttcttcataa tggaaagcca tatttcaaat ttcaatcgtc cttcttctgt aatagcaaaa
     4921 ctaaactctt tttgcagcag atctaaaaaa gtgtttcctg ataaatcact gtattctttt
     4981 ttgttttcaa gatagggacg tccaaaccaa aataattgga aataaaaata acgaatttga
     5041 ctttctggac cggtcagccg cccgcttttt attttcagat gaaactcatt taaagaatca
     5101 tttaattcct tcactctgcg agacaaagta gccgaactga tattaagttc catgacaagt
     5161 ttctgccgat caaatgagcc attttccaac agataaagaa gtatctgata ttttgtagaa
     5221 tagcgcaagt aacaatgata ataaacatcg actgctgcta tatttccatg tctgattaat
     5281 aaaatattcc cttcttggat aacaatttcc aattgtttga tattttccaa gttagcttgt
     5341 aaataaaaca gatcttcttt caaggtagcc cgatcaactg ataagcgggt aagtacgtta
     5401 ttaacggaaa cctctccttt ttcctctaat aaaagttgga aaagtttgat ttgccgttgt
     5461 tcattttttt caagcaaact gaaaagttcc aagtccactc cttcaagcat ttctgatttt
     5521 aacagcagaa gtttccttct catccttgaa aaagaggatc tgtgacagtt atcacagatc
     5581 catacaaatt ctaatcactt agctggttgg cccagctgtc agttcccatg tgaccatgcc
     5641 ttgatacgtt gcgccaggtt gagcattcgt actgctattt acctctaaga gtaaaccatc
     5701 ggtatcggga aagtcaacga gaacctctcc tttagctgtt ccatcactct cataaatcaa
     5761 cgtgttttga ccttgctgaa tcaattgctt tgatccagaa tgattgaagt ataattttgt
     5821 tccgcttaat tcttgacctt gatcattctt aaataaagaa gtcaatgtac ctgtcagttg
     5881 ccaaggagac gtgcttggaa gccgagtatc ccgcacatca aaagaaaaat cgccttttga
     5941 ttcataataa cttgtcttca acggtttggg gagatcgtta ccaaatgtaa aatcatctgg
     6001 tacattaact aatgttaatt cttgtttggg gacaagaatt gttttagcag atgtttggac
     6061 aactgggtta ggaagcttag caccatctgc taatgtaata ttgctggaaa gtacagctgc
     6121 ctttgtcagt atgctgacgg attgtaaaga agtatctaca gtatttaatt gatagttgta
     6181 agtaagatga atggtatctc ctattttgct caatagatct gctgggacag aaactccttg
     6241 ttgaatgccg gtatctgcga tagttgtacc atttaatttt aaggaaccat tgatataagt
     6301 agtattttca ggaagtgaga tcgtgatttt tttgtcggtt atcgcatccc cagattttgt
     6361 taacgtgaat gtcacatttt cttgaatcgt atctcctgat agagcagaca ggggatctcc
     6421 tgtctcaggt tgtctgctaa ggttttttag ctgactatct gcactcacag atggaaaatt
     6481 agccactgtt atttgaggaa gagtggtgat tccagcttgt tcagactgcc atttgtacgc
     6541 aaaagacgga tacatgttag taagtacatc gtccaccaag cgataatcaa aataatattg
     6601 ccctgaagcc aatgcaggta gtgtagaagt agctgtattt acacccccgt tatttgattg
     6661 gatcgtttgg aaaagttgat ctgtccatcc tgtcgctaat ttttgggttc ctttcgaata
     6721 aagcctgtaa tggatcgaac ccttttttag ttcagttgct gaccaattgg ccgtgacttg
     6781 gtacgatgca gaagaaagga gctttcccca ctgactatcg gctgttaagt ttgttggtaa
     6841 aaccgttccg tctagcttgt actgctccaa tacctttaaa gcaacaggag aaagcaatcc
     6901 gccttgcgca tctttcccac tgtacacgat attggctgtc tgattcaatc cacttgtgct
     6961 tgctggagta tgagtcagta ttgctccttg tgtaacgttt tttcctgtaa aatagttgac
     7021 tacgcgatca aagcgactaa atggcaactg atcaaaacgt ccttcagtca gccccatcgt
     7081 caatgtccga ctattggtag agcctggaag gttctggtta tttccatcca cgtattctgt
     7141 ggtatcagca aatgtattat attttcctgt tgaatcactt ggtgagatgt aactattcca
     7201 cgtatttatc tctgcacttg cagaaaatgc atatgctcct ggattcaagt ttccgccagt
     7261 taatttagga taaaatctac ctgcagctgg agctaaaatc gaattgaaca tacgatcttg
     7321 gataatatta ttcgaatcat aaaaatcaac agaaaaaacg gcctttttag acatagaagg
     7381 ttgtaattta cccttggtca aaattttgtt tggactcgta tcatatttat ctccgttttc
     7441 tgtcatcttc gcgacattag ctgtaacatc aaattgaagc gggatatcgt ccacgttgaa
     7501 ttcgcctcgc gtaataatag caataatatc tgtcaataga gtatttgtat cccacgaccc
     7561 catcccaaaa cgcaaggtat ggcttcctgc tgataataaa cttccgctag taaccttgat
     7621 gggggcaccg gcaatataca agtaggcgtt gttccagtca attgctttta atacatcact
     7681 gcttgataca tgttccggca aattgatgat cgccacaaat gcaaatcctt gtgcagtgat
     7741 attcaccaaa tcaagtacat ttgttagttt cacatggctt tcccaatgaa aacggaccgt
     7801 gccacctttc caaggatcat ttgaaccatc gttcaaataa gtagcagtca atttaggcaa
     7861 ttggttagcc ccagttagtt ggacaagctt cccgccattg actgcactac gtccaccatt
     7921 tggaggtacg atattttcat acgtgtatgg atcataagaa atggcaggag gcgttgctcc
     7981 aacagcttct gccgaaggtg aaaagataaa tatacttaaa acaaaaagcg aagtaaaaaa
     8041 gaaggagcga aaaaatgaaa atttattttg tagcaacatg ttcatcctcc ttttcctttc
     8101 ttctccataa caacaaccac gcaagaagcc ataattctat accaatgatc aaataaagaa
     8161 aacggatctt ttgttcattc gtctccggga atacgccatg ttgattagat tctttataaa
     8221 tcggtagctg tgtctgctgt tttgatgcgc cattgactga accacttgga tgcggaattg
     8281 gacttagatc ataaaatgta atcgtacctg aacttttggt atctgcttgg actggctctt
     8341 gccagctgaa gaacagcaaa cttgcaataa tccctcccaa taagatcgat gttttcaacg
     8401 aaatcacctc atcatgttgt ttctcctttt ttctttttct tgtggaccca caaaaacagc
     8461 aaaatcaacc ataacactag aactgcagca accaatcctg cataaagcca tgggaaagat
     8521 tgttcttgta ctgcaaaaac ggaattttca tttacttttt gtgctttttc ttttttgatc
     8581 gtaaagtttt gtgtaaatcg ccattcatta ctgtcgcctg attttgctat gatttctgcg
     8641 cggtaatcgc cggcttttgc tttatcacca gctaaatcaa gattcaagtg aaaattcgaa
     8701 tttggtgcaa ctaaaaatgc attagacgta ttttcaatat aaagctgatt ttcataaaag
     8761 atcttgctcg taaatatcaa attgctgata atcctaggct gaggattttg gaaacgaagc
     8821 tgaacagttg gctttgcgtt gacctcgtcc aaatctcctg ataaaagttt cagatccggt
     8881 tctatcgtct cttgactttc ttgtaaaacg actgcgagat tataagcaat acgatttttg
     8941 atttgtgctc cttctgtagc attttctgtt tcagcttctt ctttaggtga aacatgaata
     9001 cccgctaata cacgtccctt aaagcttttt gctgggattt tcacttctat aggtacagtt
     9061 gtttctgcat gtgctgatac attgatttcc tttttcgcaa tgagcaaaac gtcacggaca
     9121 tcaaacggca atgtctcatc taacgttggg tttttttgac tataatcgat cgttccacca
     9181 tcactcgtta ccgccggatt gacagagacc tcaaatgttc gttcttcatc tgaagaattg
     9241 ctgataatta cttctagtgt ctgattttgt tccggtgcca ctaataaatc aaaataaccg
     9301 attgctttat ctatttggtt ctctggaaat accggtttga ttccaaagtc tcctgctgaa
     9361 tcctttgctt ggctgattga cggaaaaatg aaaaatagac ttaaaataga aaaaaacgaa
     9421 acaaacagtc gtttactttg attcattgtc caccacatcc tttcaacaaa aactgcggta
     9481 aaaaatcctt cacctatagt attttcaccg cagtttgttg aatttatttc atactctatg
     9541 ctactgggcc agccacgagg ttccaagtca aatcagccgt gaatgttcca gctgctgctt
     9601 tcggcacttt cagtgtgata tttgctggat cataaacaga aagccatgtt cccagacctt
     9661 gatcttttgc tgctgtaaat attggtgtcg ctgcacttgc tttactcaac ccagttacag
     9721 tttgactagt tggtccagtg gattcgttat ctgacgtact tttaacagtg gatgttccaa
     9781 aatctaattc agcgccttgc aaaacttgtg aaccgtttac aaaatctgaa accgaaactg
     9841 tcacgttcca cccttgtgct tggccatctg cacctacacc tcgacgatct gatacttgga
     9901 gataaggtgt atcgtttttt gaagcgtcta cttgatacgt ttttgttccg ctttcaatct
     9961 cgtgtgtacc aaatggcaat tcgggaacta catccagtgt taaaggacct gtctggccag
    10021 ttgctggatt ttccggatct gctggctcta ctggttttga aggatcagta gggttaagag
    10081 gctccacggt atttgttggt tcagtaaagg taatatctcc ttttgaagtc gcttgaccct
    10141 cagcaaaagc tggtgttgcc aagccgccta gaagaagtgt tgaaagtaat aatgtcccaa
    10201 tcatgttctt tttcataata aatcactcct ttttcttagt gacctaagta tagtagagac
    10261 agtatgcaaa aaatcctcat gaattcatcc gcttacttga aattatgacc ctttttgtat
    10321 aaaagagaaa aaaacaataa aatcgtttac atgaatattc ttcctttttt attaagtatg
    10381 aaaaaatatt tcaattttat tttttcgttt caaattattc tattagtgac atccttgaac
    10441 tataaaaata ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttgtttct
    10501 taaataaaac gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca
    10561 gctaattgca tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct
    10621 gtacttcctc ctaaagacaa tccataaata acgggtctgc caatagcaac aagatctgca
    10681 cctgatgcga ttgctttgaa aacatgctgt ccgcgtcgta caccgctgtc aaacacgatt
    10741 ggtacacgtt tatctactgc ttctgccaca tattgcaacg aatcaaacgc tgctggacca
    10801 ccatctagtt gacggccacc atgattagat acccagattc cgccagcacc agattctaat
    10861 gaacgataga catcttcttc tgactggaca cctttcacat agactggcaa atcagaatga
    10921 gcagcaataa attctacatc ttttggacta agcttctgtt tggaagattt gtacacagca
    10981 tccatcgttt ggcctactcc tgattgataa gcctgaacga tcggcattgg cagtgggaag
    11041 gtaaacccat ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat
    11101 acgattgctt ttgctccatt acgcttggcc acttcaagaa tatccaaatt gatcccatca
    11161 tctttgctca tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc
    11221 tcttctaatg tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca
    11281 ccttttgcag aagctttctc cgccttcaca tgagctaagc catgagcagc aactggtgcc
    11341 atgatgattg gtgcagtcag cgtttctttg tcaaaatgtg tagttgtatc gggtaattct
    11401 acatctttta atacatgagg aataatcagc tgatgattga atgcccgttc attttcttga
    11461 taagtaaata agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact
    11521 tgtgaagcgg ctatttctaa atcttccata ttgataaaat ccactattcc ttcatttgta
    11581 cctgcttgat acgttttttc catttcaatg ccttccctct ataaaaaatt gaatgaataa
    11641 tatgatagcg tttacacact ttatattact cttccgtatt gatacaggca aataatctgt
    11701 ttacatttca gtcattccag ttgttactac tctgaaagaa atgtttagaa cacaaataga
    11761 taaaaaacga tgggactttg acaaaagtcg tgtcaaagtc ccttttacga aatactgtgg
    11821 atgcctattg aaataagtgc gggttgtttc tttgtatttc aatcgtttct tgccattctt
    11881 cgtttaattt tggattaccg ttttcttcct tgaacgcata aggtcccaat ccaaaaagct
    11941 cagttagtat ttgattcacg attccatata gctcggtgtg atcggacaca tgattattga
    12001 agatcatctc attatcacca cgtttgaatc tcacattcgg gctctcatga tgaacctcta
    12061 tgcagttttc cccttttttt aactgacgga tttctgaaaa catctcataa atatcttctc
    12121 caataaaacc ttcagcaagc gccagactgt ccacaagacc gcctactgtc aactcagaga
    12181 ttatcgcttt gttggcacca agctttaatg tatagcttgt tgcttctact acgccgcatg
    12241 cattgcaacc accttctgat agttcgcttt ttctgatcaa ttgaacttct ttcattttct
    12301 gttcctcctc ttagttttct acttatgtat tttttatcac ctgacgtaag tgtattttat
    12361 cgtcacgatc acgtgattta ttcttcagag attctattta tctatctaac atatccttta
    12421 ttcttctact tttctttctt tagcatataa gagatacgca tagtaatcaa gcaaggattg
    12481 ttgatataaa aagaacaaat accgctattt tgatttcttt tgactctatt actcatataa
    12541 ataaaattta ttatacctgc tctttttgac tatatcctca tctgactcct atacttcgtt
    12601 tgtattaaaa cttaggagga atcattcatg cttacagggc taaacttatt aaataatcct
    12661 tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatacgg aatcactggt
    12721 atgcttccaa gtactgtcca aacattggaa caacaatctg tccaagctta cggtcaatac
    12781 ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa tgaatctatt caatacgaac
    12841 cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc tgtcgtttat
    12901 gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa accacaagat
    12961 gctgcatttc tttcaatcga cgaaccagaa aatatcaaag caagtttgaa aaacgcagca
    13021 gatggacgcg acattcgttt gatcgttgtg acagatgcag aaggaatctt aggtatgggt
    13081 gactggggtg tgaacggtgt tgacatcgct atcggtaaat tgatggtcta cactgctgcg
    13141 gctggtatca atcctgcaca agtcttgcct gtatcaatcg acgctggtac taataacgaa
    13201 gaattattga ataatccatt atacttaggg aatcgtcatg ctcgtgtaga aggcgaaact
    13261 tactacgaat tcattgatca attcgttcaa tcagctactg aattattccc agaattgtta
    13321 ttacattggg aagattttgg acgaggaaat gctgcaacga tcttagaaaa atacgaagat
    13381 aaaatcacta ctttcaatga tgatatccaa ggcacaggaa tcgttgtact agctggtgta
    13441 ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt gacatttggt
    13501 gctggtactg ctggtgtagg tatcgcaaac atccttttag atgaaatgat ccgtcaaggt
    13561 gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaggg tttattgttt
    13621 gaagatacaa aaggattgac tcctggtcaa atcccattcg ctcgtaaacg ctcagaattc
    13681 actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcaatcca tccaacgatc
    13741 atgatcggta cttctaccca acctggagca ttcactgaag aaatcgtgaa agaaatggcg
    13801 gctcataccc cacgtccaat catcatgcct ttgtctaatc caacaaaatt ggcagaagca
    13861 aaagcaaaag atttgattga atggacggac ggaaaagctt tagttggaac aggtatccct
    13921 gctgctgatg tggaatataa cggtgtaact taccaaatcg gtcaagcaaa taatgcgttg
    13981 atgtacccag gtttagggct tggtttgatt gcttcaacag ctacacgagt aaatgctgaa
    14041 atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa accaggagca
    14101 gctatcttgc ctccagtagc aaaaatcact gaattctctc aaacgattgc tgaaactgtc
    14161 gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat caaagaagct
    14221 gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga ttgttatagt
    14281 aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact gcggcaaaac
    14341 agcagccagc caactactgc tcttttgccg cttctctttg gcttttttca tacgatacag
    14401 aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc agttgaggaa
    14461 aagaaaaaag aagggttttg gtcaacaaaa attaccggcg taagtttgcc cctttatttg
    14521 atcatggtcc ttgtcctgat cattgcaatg gctttaggaa aattaccgac gaatatgatt
    14581 ggcccaatcg ctgttttggt cattttcggt aatttatttc attttatcgg aacaaaaatc
    14641 cctatcgtca aaagttattt aggtggtggc tccgtttttg cgatctttgc ctctgccgcg
    14701 attgctacat ttgggatctt acctgagtat gtagtaaaat ccacagaaaa ctttgtcagc
    14761 aatatgggct ttttagactt ttatattgct gccttgatta ctggtagcat ccttggtatg
    14821 aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact tatttcaatg
    14881 gtcgtctcct tttttgccgt aggactcgta ggtatgctta tcggaaatgg ttttgcaaat
    14941 tccgttttat atatctcatt gcccgcaatg gctggtggtg ttggagccgg agctgtacct
    15001 ttatcaacga tttatgcaaa tgttttaggt acggacgctt cttctatcat ttcccgcctg
    15061 attcctgcga cagcaatgac aaatgtccta gctatcattg gtgcagcgtt agttgctcgt
    15121 gcgggacaat caatgccaaa attcaacggt aatggtaaat taatggaaaa aggcgacctt
    15181 ggcgatggaa aacctagaaa tgtaaaacct tccattccac aattaggtgt cggtttgatg
    15241 gtatcattga ctttcttcat cttaggacaa atctgtaact attttgttcc taaagtccat
    15301 gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct attaccagaa
    15361 tattacgaag atgcagctat catgttcaac aacttgattg tcaaaaactt gacagcagct
    15421 gttcttgccg gtatcggtat tgcgttattg aatctaaatg tattggcttc tgctttgaca
    15481 tggcaatttg ttgtcttatg tttgacaagt gttatcgcga tttcagtagt ttcaggattt
    15541 gtcggtcgtt tgtttggttt atatccaatc gaatcaacta ttactgctgg cttatgcaac
    15601 aactcgatgg gtggtacagg aaatgtagct gtcttatctg cagctaatcg gatggaactt
    15661 attgcttttg ctcaaatggg caatcggctt ggtggtgcca tcgttttgat catctcaggt
    15721 ttcttgatgc aattactttc ttagtcaaaa aaaatacctg atcatcaagt atcggcttga
    15781 tgaccaggta ttttttgata aaaatttaca ggattttcaa aaatcttttt tgcattttat
    15841 cggaatgata accgatgcgt tcataaaatc ggtgtgcttc tactctgtat tctgcagaat
    15901 taagtcttat tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc atcaaggcct
    15961 ttccgattcc tcttttttca gcatgtttag acacagcaag tgccataata ttgaacatcg
    16021 gatcgctgaa aatcgattca taggactccg cttgtacata gccagctgct tttttagaag
    16081 taggatcttc atagaccaag aaaaaatttt tttctggctc cttcaacagt ttcttcattt
    16141 gtctcaccgt aacggttaat ggtacatcat atcctaattg ttccgcattg atttctcgta
    16201 gtgcttctgc atctcctaaa cgcatttccc gcaacatcca aatcaccagc caatttttat
    16261 ttttttaaat gaattttttt atcacagtat actccttttt tcatttagaa caatatgttt
    16321 cagtgatttt gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg acgaaagcat
    16381 tgtaaagatt gatcaaaaga accacgagga caattacttg aaaaataaag gtcacttttt
    16441 ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca gcagcgggga
    16501 taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa gcaatcgtta
    16561 ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt gcttctttta
    16621 ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca ccaataccta
    16681 aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc catgacgtca
    16741 aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag gtcaataccg
    16801 tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt aaaaaaactt
    16861 cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc caaccgacaa
    16921 tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca atggacatcg
    16981 tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg ccgataagat
    17041 caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca actgtgttcg
    17101 ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt tctcttgttt
    17161 atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa caaacgaggc
    17221 ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaaaagag gttgtgaaaa
    17281 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagctcctca taattttcgt
    17341 ttggttgaac ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg
    17401 agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    17461 ttgaatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagagact
    17521 gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag ttcttcatat
    17581 ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt atttggaaaa
    17641 agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta attcattctt
    17701 ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa ctataaaggt
    17761 tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt tttcaagcga
    17821 tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt gggggttttt
    17881 aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa tccacttttt
    17941 caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta aatagatttg
    18001 actctggact tataaagtct acttcacctt ctagtattac tttcttatgc aaatattcct
    18061 ctaataaata tagacttgct aaagcataca acacattttc caaatttgct tccttataat
    18121 ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt gtatcttcag
    18181 gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata accctaacac
    18241 cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca gttatgcttt
    18301 tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac tccgaaccaa
    18361 tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc tcatcgagca
    18421 tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac ttccaataaa
    18481 aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac aaaaaaaaca
    18541 cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa ccatccaatc
    18601 agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt cttagtccag
    18661 catgtttgca accacaccta caattttcag tccaacaata gatttcgact agctaagtct
    18721 tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa tacccacaac
    18781 gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta taatctttaa
    18841 acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa gctactttca
    18901 tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac gttccttcaa
    18961 gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca ttagcccaac
    19021 attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt acccgttttg
    19081 tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt ggcataataa
    19141 gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc gctgctggct
    19201 tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc aaattgctga
    19261 taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat atattcgcct
    19321 gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat ccgcatactt
    19381 ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag gcagtgggat
    19441 aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa actcaatata
    19501 cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg caagtccttg
    19561 tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag caacaaagat
    19621 agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat tggcaaacaa
    19681 tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag gtgctgtcac
    19741 atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt ggtaatatcc
    19801 gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat tgtatagttg
    19861 tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata aatccatgat
    19921 atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct cttgtacaat
    19981 attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag cacgagtttc
    20041 tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt cagcaagttc
    20101 agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac cttgtccatt
    20161 tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt tcaaatcaaa
    20221 gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag tgactccatc
    20281 acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg ttttttcgtt
    20341 atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag tggattctaa
    20401 gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag aacgcttatt
    20461 tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt cattaagtgc
    20521 tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga atgaatctaa
    20581 tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat tctcatacac
    20641 atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg tatcatcgac
    20701 attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat cgtgtccata
    20761 taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa tcacttctac
    20821 aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg aaatagtttt
    20881 gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg ctgttagctc
    20941 agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt gtaactcctt
    21001 cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc aaacttgcta
    21061 gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc tcattaatcg
    21121 tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca ggatcagtct
    21181 ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct aattcactca
    21241 caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta ctttgaacgg
    21301 tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac gccttggcaa
    21361 tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg tttatttcta
    21421 atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta tctccactac
    21481 ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat ccgtctaaat
    21541 ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag aaattgtgca
    21601 atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa agtggtggta
    21661 atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta gacttcacac
    21721 gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga gtaaccgtta
    21781 cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt ttaatctctg
    21841 caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa tgccaaccat
    21901 ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca gcttgtggca
    21961 tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata attcgaacgg
    22021 ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg gtgtaatcat
    22081 ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt tgtctcgctt
    22141 taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc gctaccccaa
    22201 taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag gtgatagcaa
    22261 ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta gaaagaaaat
    22321 tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt gaagtaatcc
    22381 attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt actgttcttt
    22441 gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt cactgcatgg
    22501 gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc tctaaaagtt
    22561 cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg gtagttggct
    22621 ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat ccccatacat
    22681 tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc atagtccacg
    22741 attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc tcgataaggc
    22801 aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa gctatgtgaa
    22861 tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt taaatcatct
    22921 gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg tggttctact
    22981 tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg tctccataaa
    23041 ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag gttcacatca
    23101 tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat cggattggct
    23161 ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca tatcgctata
    23221 aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa gtattcctcg
    23281 tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa gccgttcttt
    23341 tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc ttcgtgccac
    23401 tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc actagccagt
    23461 gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat tttcagttgt
    23521 ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag catatcatag
    23581 cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc ttctgctggt
    23641 tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt tttcccattc
    23701 ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc tttagtacgc
    23761 cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat tgctttaccg
    23821 tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc ttcatcgaac
    23881 acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg ctcacacgct
    23941 tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat gtaatcattc
    24001 atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc ttgttcaaag
    24061 ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt ttcaaatcac
    24121 tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg ttttcgcatg
    24181 tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga gcaaaggcta
    24241 aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct ttttttaatg
    24301 ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca gggggcgtga
    24361 tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg attgcttttt
    24421 cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc atgttgtcct
    24481 cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt cgtttttcga
    24541 tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct gcatttgaat
    24601 ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt tatcagtaat
    24661 aacaattgta tttttcaata aatctcctac tttcattata tactttttca cttgtgatta
    24721 caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta tttgtaccaa
    24781 aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact caaagggtac
    24841 tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca tggcataaag
    24901 aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca attttgtgat
    24961 ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat actggatgat
    25021 ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat tgaaaatatt
    25081 taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct ttatgcttcc
    25141 cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta cattgttttt
    25201 taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct tgttcccatt
    25261 ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct ttaaaattaa
    25321 ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga gtgtttgaat
    25381 actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata attattctac
    25441 ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga acttatcatg
    25501 gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg ctcgatcata
    25561 taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat agttgccact
    25621 atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct gcagccaatc
    25681 aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat cttctgttac
    25741 caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat ctgtatattg
    25801 aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc cttgattcgt
    25861 aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa cggggacaag
    25921 ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt ccccagcata
    25981 ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac caaattgctg
    26041 tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga cccgttacta
    26101 cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt gacttattgt
    26161 aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca ggaatactgc
    26221 taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat aaatcctttg
    26281 cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca actaaataaa
    26341 actcttgttg catagtagct ccattataga aatgctgtaa atttttctca ttacatttac
    26401 tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct tcccatttat
    26461 acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc tccatcttgg
    26521 catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg ggactaaaat
    26581 agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga agaaaatatt
    26641 catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa ggataaaaac
    26701 ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca ataaatacac
    26761 attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta tcgatcttgg
    26821 cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat aattctccaa
    26881 gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca cctagcgaat
    26941 ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta ctcttaccaa
    27001 tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt ttatagatac
    27061 gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg ttgtcgtcta
    27121 cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat ttaacttttt
    27181 cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact actgctttgt
    27241 gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc gttaaggcat
    27301 cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa tcttttaata
    27361 ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg ctatcattgg
    27421 ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct ttattgcatc
    27481 ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg ctggcttcgg
    27541 gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc cctcaaatct
    27601 tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg tggttcattg
    27661 tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc gtgccatagt
    27721 aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc gccttgaaca
    27781 atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg agtctttatt
    27841 tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc aatattattt
    27901 agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat aatcatagtt
    27961 ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt acctttatca
    28021 tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac accatcagga
    28081 attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt agaaggaaat
    28141 ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt ttcagaatgt
    28201 tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat atttctagtt
    28261 gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc attccaacca
    28321 ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga ccatcccgac
    28381 ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct cctttttcaa
    28441 aatactggta taaatcattc agacacctcc atgagcttca actgctcaag ttcgtatttc
    28501 aacccatccc gaacaaattc agcacctttt aattgattct gatactttac ccatgtatta
    28561 aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt taaaatctga
    28621 ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt tttgagtaca
    28681 tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc gatattattt
    28741 tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag ttggtttaat
    28801 agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc gtactcgtca
    28861 taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc aattaatggt
    28921 aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg tttcataatg
    28981 gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt tttatcccaa
    29041 ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt ctttgttctc
    29101 gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa attttctcac
    29161 tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact gtctcatcta
    29221 aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt accattttgg
    29281 aatgaccata aatagttgat acagctttat tggctatcac attcgcttta atgtagtcta
    29341 ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt tggtgttctt
    29401 tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt agttctttga
    29461 tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt ttgaatgcca
    29521 tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc caatgaatcg
    29581 atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct agtaacctgt
    29641 ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc attaatatcg
    29701 acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc tccagttcaa
    29761 gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga tactcttcaa
    29821 tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc aatactccgc
    29881 ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac aaacattcaa
    29941 acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg tcttctgtaa
    30001 gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt attatttcct
    30061 cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc cataaagcga
    30121 tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt taaaccgtta
    30181 ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc agcttgtgcc
    30241 ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa cgttccttga
    30301 gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg atcatcaagc
    30361 gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac atttctatac
    30421 aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct tataaactca
    30481 atataccatc ataccttttc atttgcaaga attttaacat acttttttga acttttatgt
    30541 ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg tgaaaatgtg
    30601 gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact ggctagaatt
    30661 gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc cagtaaaggt
    30721 gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct taaacaactt
    30781 tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac attaagagat
    30841 atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa aaaaactatt
    30901 gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa catcgaagaa
    30961 gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata tcttaaaaca
    31021 ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc agctcttcta
    31081 aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac agatgagaaa
    31141 aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc tcttgttact
    31201 gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg cccccacggc
    31261 ttgctacact atggagggaa aactattatg gcaacattca agcaatatga aactaaaaaa
    31321 ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg caaacctgat
    31381 aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt gtcacgcttg
    31441 caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt taaagaagtt
    31501 tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt catgaaaact
    31561 gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt gaatgaagtc
    31621 acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga ccaatacaag
    31681 gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg gattattgag
    31741 actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca taaagaaaag
    31801 gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa cagcaaacct
    31861 gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta tagcggggtt
    31921 aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga aaatacggtc
    31981 accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga tcctaaaact
    32041 gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt aaaaaaatgg
    32101 cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac tatgattttt
    32161 tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt gattactatt
    32221 tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac tcatgcttca
    32281 atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg gcatacaaaa
    32341 attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga aaagactgtt
    32401 gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc atacagtaaa
    32461 aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat agtctattct
    32521 tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc tgcgtctgtt
    32581 tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt
    32641 aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata gttacgtcca
    32701 aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt tccttggtca
    32761 tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg ttctgtcata
    32821 agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc taaataattt
    32881 tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc tcttgaagta
    32941 tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc atcttgataa
    33001 tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga agcttctgcc
    33061 cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg gtctacttca
    33121 tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc ttcattgatc
    33181 cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc aacacctact
    33241 gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga tttgagaggt
    33301 agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc aaatttcgag
    33361 catcacgata acgaggaatg taggacgggt ctccagaaag caagtagccg acaatctgat
    33421 tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat gtttcgctca
    33481 cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta aaacccatga
    33541 ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt attttactcg
    33601 aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag aaacactatt
    33661 tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca taaagtatac
    33721 gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca taaaaaattc
    33781 gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta
    33841 gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc
    33901 tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag gagtgacacg
    33961 gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt cgtgagcaag
    34021 catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt tgttattttg
    34081 accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc cgtcacttaa
    34141 tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt cagtagggaa
    34201 caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat caatatttga
    34261 atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt ccggctgatt
    34321 cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga
    34381 agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg aatctttttg
    34441 tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca cgatcgcctt
    34501 ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat cttaacatat
    34561 gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa acctgtttta
    34621 agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt caccactcta
    34681 tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa tacactgatt
    34741 tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa
    34801 gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg cagagcagct
    34861 ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag
    34921 ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc tcttttattc
    34981 cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa
    35041 tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt tgctggacac
    35101 ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc gggtagaaga
    35161 tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct atgtcactca
    35221 aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa
    35281 ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta aatatgttgc
    35341 ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc gagaccgttc
    35401 tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa caattatggt
    35461 gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag gattgggata
    35521 cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg aatttttatc
    35581 ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag gaagcaatca
    35641 ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc
    35701 tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa cagaagcgat
    35761 gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt tttattagga
    35821 aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt tagcgactgc
    35881 ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg gtctttctgg
    35941 tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg ctgcttttgg
    36001 ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta aacgtttgaa
    36061 aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg gtggtttagc
    36121 ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc attatgcggt
    36181 ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag aagtctttaa
    36241 ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg
    36301 gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt tcagccttga
    36361 gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa taagaagtaa
    36421 ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca taaaattgac
    36481 ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg
    36541 atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg ttgataataa
    36601 acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt cactaaccaa
    36661 aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact gatgatcaat
    36721 gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa taattggtta
    36781 atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg tagtgacaat
    36841 aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa aggcagctga
    36901 aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa tatccatccg
    36961 aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg gataaacaga
    37021 aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt aactgttaag
    37081 aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc taaatcagtt
    37141 aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt taacaaggaa
    37201 gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt gtaactaaat
    37261 aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa ctcactgttt
    37321 gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc aaggaaaaat
    37381 cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga aagtggctga
    37441 ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt taactcattt
    37501 tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat cagcatcgag
    37561 ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc tgttcgagcc
    37621 aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag agaaaacaaa
    37681 aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc ggttaaaata
    37741 aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac caaaatcttt
    37801 gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag gtcatttcct
    37861 tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat accaaaaagt
    37921 gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa catagcaaat
    37981 ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag atagatagtt
    38041 aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg attaacatat
    38101 aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc catctcctgt
    38161 cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag gtgagccatt
    38221 caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa tagccagttt
    38281 atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg tttttccttt
    38341 tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat caagtccttg
    38401 caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga tcaaaatcgt
    38461 tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac tatacatatc
    38521 aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta attcataggc
    38581 agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat ctggggaatc
    38641 aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt tcattccatc
    38701 tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa tcgtagttga
    38761 ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga
    38821 caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga attcaatcat
    38881 ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt ggtggagtaa
    38941 atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta cttgggttgt
    39001 tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt tttctttaaa
    39061 atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata cttctctttc
    39121 ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg ctaatagatg
    39181 taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac tcatattcga
    39241 gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct ttcaatacta
    39301 ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca tcgtaatgga
    39361 tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa
    39421 caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat
    39481 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
    39541 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
    39601 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
    39661 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
    39721 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
    39781 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
    39841 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
    39901 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
    39961 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
    40021 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
    40081 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
    40141 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
    40201 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
    40261 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
    40321 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
    40381 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
    40441 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
    40501 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
    40561 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
    40621 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
    40681 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
    40741 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
    40801 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
    40861 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
    40921 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
    40981 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
    41041 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
    41101 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
    41161 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
    41221 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
    41281 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
    41341 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
    41401 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
    41461 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
    41521 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
    41581 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
    41641 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
    41701 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
    41761 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
    41821 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
    41881 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
    41941 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
    42001 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
    42061 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
    42121 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
    42181 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
    42241 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
    42301 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
    42361 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
    42421 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
    42481 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
    42541 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
    42601 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
    42661 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
    42721 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
    42781 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
    42841 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
    42901 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
    42961 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
    43021 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
    43081 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
    43141 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
    43201 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
    43261 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
    43321 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
    43381 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
    43441 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
    43501 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
    43561 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
    43621 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
    43681 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
    43741 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
    43801 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
    43861 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
    43921 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
    43981 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
    44041 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
    44101 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
    44161 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
    44221 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
    44281 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
    44341 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
    44401 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
    44461 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
    44521 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
    44581 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
    44641 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
    44701 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
    44761 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
    44821 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
    44881 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
    44941 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
    45001 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
    45061 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
    45121 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
    45181 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
    45241 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
    45301 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
    45361 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
    45421 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
    45481 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
    45541 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
    45601 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
    45661 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
    45721 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
    45781 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
    45841 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
    45901 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
    45961 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
    46021 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
    46081 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
    46141 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
    46201 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
    46261 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
    46321 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
    46381 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
    46441 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
    46501 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
    46561 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
    46621 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
    46681 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
    46741 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
    46801 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
    46861 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
    46921 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
    46981 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
    47041 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
    47101 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
    47161 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
    47221 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
    47281 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
    47341 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
    47401 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
    47461 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
    47521 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
    47581 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
    47641 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
    47701 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
    47761 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
    47821 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
    47881 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
    47941 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
    48001 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
    48061 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
    48121 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
    48181 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
    48241 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
    48301 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
    48361 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
    48421 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
    48481 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
    48541 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
    48601 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
    48661 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
    48721 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
    48781 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
    48841 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
    48901 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
    48961 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
    49021 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
    49081 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
    49141 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
    49201 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
    49261 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
    49321 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
    49381 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
    49441 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    49501 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    49561 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    49621 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    49681 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    49741 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    49801 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    49861 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    49921 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    49981 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    50041 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    50101 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    50161 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    50221 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    50281 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    50341 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    50401 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    50461 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    50521 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    50581 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    50641 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    50701 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    50761 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    50821 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    50881 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    50941 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    51001 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    51061 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    51121 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    51181 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    51241 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    51301 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    51361 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    51421 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    51481 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    51541 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    51601 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    51661 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    51721 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    51781 tgtgccactt gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact
    51841 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    51901 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    51961 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    52021 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    52081 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    52141 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    52201 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    52261 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    52321 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    52381 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    52441 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    52501 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    52561 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    52621 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    52681 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    52741 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    52801 tcaagttctt atataaaacg ctttcactta
//
LOCUS       DAYYKC010000009        49461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000009 DAYYKC010000000
VERSION     DAYYKC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.009"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..636
                     /locus_tag="ACHBBW_000554"
     CDS             265..636
                     /locus_tag="ACHBBW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9152264.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(910..1602)
                     /locus_tag="ACHBBW_000555"
     CDS             complement(910..1602)
                     /locus_tag="ACHBBW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9152265.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1605..2390)
                     /locus_tag="ACHBBW_000556"
     CDS             complement(1605..2390)
                     /locus_tag="ACHBBW_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9152266.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2627..4027
                     /gene="lacG"
                     /locus_tag="ACHBBW_000557"
     CDS             2627..4027
                     /gene="lacG"
                     /locus_tag="ACHBBW_000557"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9152267.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4052..4369
                     /locus_tag="ACHBBW_000558"
     CDS             4052..4369
                     /locus_tag="ACHBBW_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9152268.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4382..6055
                     /locus_tag="ACHBBW_000559"
     CDS             4382..6055
                     /locus_tag="ACHBBW_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9152269.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6069..7700
                     /locus_tag="ACHBBW_000560"
     CDS             6069..7700
                     /locus_tag="ACHBBW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9152270.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8191..9756)
                     /gene="guaA"
                     /locus_tag="ACHBBW_000561"
     CDS             complement(8191..9756)
                     /gene="guaA"
                     /locus_tag="ACHBBW_000561"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9152271.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9815..10738)
                     /gene="coaA"
                     /locus_tag="ACHBBW_000562"
     CDS             complement(9815..10738)
                     /gene="coaA"
                     /locus_tag="ACHBBW_000562"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9152272.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10934..11539
                     /locus_tag="ACHBBW_000563"
     CDS             10934..11539
                     /locus_tag="ACHBBW_000563"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9152273.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11847..12863
                     /locus_tag="ACHBBW_000564"
     CDS             11847..12863
                     /locus_tag="ACHBBW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9152274.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13004..14305
                     /locus_tag="ACHBBW_000565"
     CDS             13004..14305
                     /locus_tag="ACHBBW_000565"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9152275.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14351..15013
                     /gene="deoC"
                     /locus_tag="ACHBBW_000566"
     CDS             14351..15013
                     /gene="deoC"
                     /locus_tag="ACHBBW_000566"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9152276.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15034..15432
                     /locus_tag="ACHBBW_000567"
     CDS             15034..15432
                     /locus_tag="ACHBBW_000567"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9152277.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15498..16580
                     /locus_tag="ACHBBW_000568"
     CDS             15498..16580
                     /locus_tag="ACHBBW_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9152278.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16792..18354
                     /locus_tag="ACHBBW_000569"
     CDS             16792..18354
                     /locus_tag="ACHBBW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152279.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18347..19480
                     /locus_tag="ACHBBW_000570"
     CDS             18347..19480
                     /locus_tag="ACHBBW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152280.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19480..20436
                     /locus_tag="ACHBBW_000571"
     CDS             19480..20436
                     /locus_tag="ACHBBW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152281.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20535..21701
                     /gene="deoB"
                     /locus_tag="ACHBBW_000572"
     CDS             20535..21701
                     /gene="deoB"
                     /locus_tag="ACHBBW_000572"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9152282.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21725..22543
                     /locus_tag="ACHBBW_000573"
     CDS             21725..22543
                     /locus_tag="ACHBBW_000573"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9152283.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22560..23267
                     /gene="deoD"
                     /locus_tag="ACHBBW_000574"
     CDS             22560..23267
                     /gene="deoD"
                     /locus_tag="ACHBBW_000574"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9152284.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23565..24251)
                     /gene="gpmA"
                     /locus_tag="ACHBBW_000575"
     CDS             complement(23565..24251)
                     /gene="gpmA"
                     /locus_tag="ACHBBW_000575"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9152285.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24423..25004)
                     /locus_tag="ACHBBW_000576"
     CDS             complement(24423..25004)
                     /locus_tag="ACHBBW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9152286.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25137..25820)
                     /gene="rpiA"
                     /locus_tag="ACHBBW_000577"
     CDS             complement(25137..25820)
                     /gene="rpiA"
                     /locus_tag="ACHBBW_000577"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9152287.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26139..26552
                     /gene="rpsL"
                     /locus_tag="ACHBBW_000578"
     CDS             26139..26552
                     /gene="rpsL"
                     /locus_tag="ACHBBW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9152288.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26636..27106
                     /gene="rpsG"
                     /locus_tag="ACHBBW_000579"
     CDS             26636..27106
                     /gene="rpsG"
                     /locus_tag="ACHBBW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9152289.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27200..29287
                     /gene="fusA"
                     /locus_tag="ACHBBW_000580"
     CDS             27200..29287
                     /gene="fusA"
                     /locus_tag="ACHBBW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9152290.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29459..30646
                     /gene="tuf"
                     /locus_tag="ACHBBW_000581"
     CDS             29459..30646
                     /gene="tuf"
                     /locus_tag="ACHBBW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9152291.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31053..31361
                     /gene="rpsJ"
                     /locus_tag="ACHBBW_000582"
     CDS             31053..31361
                     /gene="rpsJ"
                     /locus_tag="ACHBBW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9152292.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31392..32021
                     /gene="rplC"
                     /locus_tag="ACHBBW_000583"
     CDS             31392..32021
                     /gene="rplC"
                     /locus_tag="ACHBBW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9152293.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32049..32672
                     /gene="rplD"
                     /locus_tag="ACHBBW_000584"
     CDS             32049..32672
                     /gene="rplD"
                     /locus_tag="ACHBBW_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9152294.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32672..32962
                     /gene="rplW"
                     /locus_tag="ACHBBW_000585"
     CDS             32672..32962
                     /gene="rplW"
                     /locus_tag="ACHBBW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9152295.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33001..33834
                     /gene="rplB"
                     /locus_tag="ACHBBW_000586"
     CDS             33001..33834
                     /gene="rplB"
                     /locus_tag="ACHBBW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9152296.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33877..34155
                     /gene="rpsS"
                     /locus_tag="ACHBBW_000587"
     CDS             33877..34155
                     /gene="rpsS"
                     /locus_tag="ACHBBW_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9152297.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34178..34525
                     /gene="rplV"
                     /locus_tag="ACHBBW_000588"
     CDS             34178..34525
                     /gene="rplV"
                     /locus_tag="ACHBBW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9152298.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34539..35195
                     /gene="rpsC"
                     /locus_tag="ACHBBW_000589"
     CDS             34539..35195
                     /gene="rpsC"
                     /locus_tag="ACHBBW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9152299.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35198..35632
                     /gene="rplP"
                     /locus_tag="ACHBBW_000590"
     CDS             35198..35632
                     /gene="rplP"
                     /locus_tag="ACHBBW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9152300.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35622..35810
                     /gene="rpmC"
                     /locus_tag="ACHBBW_000591"
     CDS             35622..35810
                     /gene="rpmC"
                     /locus_tag="ACHBBW_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9152301.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35835..36101
                     /gene="rpsQ"
                     /locus_tag="ACHBBW_000592"
     CDS             35835..36101
                     /gene="rpsQ"
                     /locus_tag="ACHBBW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9152302.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36159..36527
                     /gene="rplN"
                     /locus_tag="ACHBBW_000593"
     CDS             36159..36527
                     /gene="rplN"
                     /locus_tag="ACHBBW_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9152303.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36564..36872
                     /gene="rplX"
                     /locus_tag="ACHBBW_000594"
     CDS             36564..36872
                     /gene="rplX"
                     /locus_tag="ACHBBW_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9152304.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36900..37439
                     /gene="rplE"
                     /locus_tag="ACHBBW_000595"
     CDS             36900..37439
                     /gene="rplE"
                     /locus_tag="ACHBBW_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9152305.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37458..37643
                     /locus_tag="ACHBBW_000596"
     CDS             37458..37643
                     /locus_tag="ACHBBW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9152306.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37679..38077
                     /gene="rpsH"
                     /locus_tag="ACHBBW_000597"
     CDS             37679..38077
                     /gene="rpsH"
                     /locus_tag="ACHBBW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9152307.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38109..38645
                     /gene="rplF"
                     /locus_tag="ACHBBW_000598"
     CDS             38109..38645
                     /gene="rplF"
                     /locus_tag="ACHBBW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9152308.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38806..39162
                     /gene="rplR"
                     /locus_tag="ACHBBW_000599"
     CDS             38806..39162
                     /gene="rplR"
                     /locus_tag="ACHBBW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9152309.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39183..39683
                     /gene="rpsE"
                     /locus_tag="ACHBBW_000600"
     CDS             39183..39683
                     /gene="rpsE"
                     /locus_tag="ACHBBW_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9152310.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39698..39877
                     /gene="rpmD"
                     /locus_tag="ACHBBW_000601"
     CDS             39698..39877
                     /gene="rpmD"
                     /locus_tag="ACHBBW_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9152311.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39921..40361
                     /gene="rplO"
                     /locus_tag="ACHBBW_000602"
     CDS             39921..40361
                     /gene="rplO"
                     /locus_tag="ACHBBW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9152312.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40361..41656
                     /gene="secY"
                     /locus_tag="ACHBBW_000603"
     CDS             40361..41656
                     /gene="secY"
                     /locus_tag="ACHBBW_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9152313.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41718..42365
                     /locus_tag="ACHBBW_000604"
     CDS             41718..42365
                     /locus_tag="ACHBBW_000604"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9152314.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42556..42774
                     /gene="infA"
                     /locus_tag="ACHBBW_000605"
     CDS             42556..42774
                     /gene="infA"
                     /locus_tag="ACHBBW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9152315.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42807..42923
                     /gene="rpmJ"
                     /locus_tag="ACHBBW_000606"
     CDS             42807..42923
                     /gene="rpmJ"
                     /locus_tag="ACHBBW_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9152316.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42941..43306
                     /gene="rpsM"
                     /locus_tag="ACHBBW_000607"
     CDS             42941..43306
                     /gene="rpsM"
                     /locus_tag="ACHBBW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9152317.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43334..43723
                     /gene="rpsK"
                     /locus_tag="ACHBBW_000608"
     CDS             43334..43723
                     /gene="rpsK"
                     /locus_tag="ACHBBW_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9152318.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43803..44741
                     /locus_tag="ACHBBW_000609"
     CDS             43803..44741
                     /locus_tag="ACHBBW_000609"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9152319.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44769..45149
                     /gene="rplQ"
                     /locus_tag="ACHBBW_000610"
     CDS             44769..45149
                     /gene="rplQ"
                     /locus_tag="ACHBBW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9152320.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45421..46260
                     /locus_tag="ACHBBW_000611"
     CDS             45421..46260
                     /locus_tag="ACHBBW_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9152321.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46236..47105
                     /locus_tag="ACHBBW_000612"
     CDS             46236..47105
                     /locus_tag="ACHBBW_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9152322.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47095..47892
                     /locus_tag="ACHBBW_000613"
     CDS             47095..47892
                     /locus_tag="ACHBBW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9152323.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48095..48841
                     /gene="truA"
                     /locus_tag="ACHBBW_000614"
     CDS             48095..48841
                     /gene="truA"
                     /locus_tag="ACHBBW_000614"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9152324.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        15663 a         9015 c        10832 g        13951 t
ORIGIN      
        1 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
       61 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
      121 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
      181 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
      241 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
      301 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
      361 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
      421 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
      481 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
      541 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
      601 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
      661 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
      721 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
      781 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
      841 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
      901 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
      961 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
     1021 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
     1081 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
     1141 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
     1201 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
     1261 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
     1321 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
     1381 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
     1441 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
     1501 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
     1561 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
     1621 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
     1681 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
     1741 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
     1801 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
     1861 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
     1921 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
     1981 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
     2041 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
     2101 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
     2161 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
     2221 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
     2281 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
     2341 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
     2401 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
     2461 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
     2521 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
     2581 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
     2641 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
     2701 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
     2761 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
     2821 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
     2881 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
     2941 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
     3001 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
     3061 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
     3121 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
     3181 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
     3241 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
     3301 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
     3361 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
     3421 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
     3481 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
     3541 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
     3601 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
     3661 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
     3721 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
     3781 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
     3841 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
     3901 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
     3961 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
     4021 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
     4081 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
     4141 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
     4201 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
     4261 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
     4321 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
     4381 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
     4441 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
     4501 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
     4561 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
     4621 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
     4681 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
     4741 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
     4801 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
     4861 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
     4921 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
     4981 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
     5041 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
     5101 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
     5161 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
     5221 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
     5281 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
     5341 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
     5401 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
     5461 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
     5521 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
     5581 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
     5641 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
     5701 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
     5761 actttgtgct ggcgcaggga caagtgggat gttagctaat gctttaaaaa aaggtgcatc
     5821 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
     5881 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
     5941 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
     6001 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
     6061 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
     6121 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
     6181 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
     6241 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
     6301 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
     6361 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
     6421 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
     6481 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
     6541 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
     6601 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
     6661 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
     6721 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
     6781 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
     6841 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
     6901 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
     6961 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
     7021 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
     7081 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
     7141 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
     7201 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
     7261 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
     7321 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
     7381 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
     7441 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
     7501 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
     7561 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
     7621 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
     7681 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
     7741 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
     7801 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
     7861 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
     7921 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
     7981 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
     8041 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
     8101 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
     8161 gggattcccc ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt
     8221 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
     8281 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
     8341 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
     8401 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
     8461 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
     8521 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
     8581 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
     8641 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
     8701 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
     8761 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
     8821 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
     8881 gtctaagaag cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag
     8941 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
     9001 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
     9061 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
     9121 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
     9181 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
     9241 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
     9301 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
     9361 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
     9421 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
     9481 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
     9541 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
     9601 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
     9661 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
     9721 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
     9781 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
     9841 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
     9901 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
     9961 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    10021 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    10081 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    10141 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    10201 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    10261 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    10321 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    10381 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    10441 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    10501 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    10561 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    10621 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    10681 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    10741 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    10801 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    10861 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    10921 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    10981 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    11041 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    11101 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    11161 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    11221 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    11281 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    11341 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    11401 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    11461 aatggaagaa acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat
    11521 caaaagtgtg aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg
    11581 cgtgcatgta cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa
    11641 ctattttttc ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa
    11701 ataagataca gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca
    11761 aaaaacatac tttccttttt tctctattct tttcttgggg gttttggtac actgataggg
    11821 aatttagata gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg
    11881 caggcgtatc agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg
    11941 ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt
    12001 tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca
    12061 ctgatttctt cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct
    12121 atgtgatcat gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag
    12181 agctttccca ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga
    12241 aatatgcatt agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat
    12301 tgaatcgtcg ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg
    12361 tttcgctgtt tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat
    12421 actatcaatt agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc
    12481 cttttaatgg gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat
    12541 caaaaaaatt actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg
    12601 cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag
    12661 tcataggatt cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc
    12721 agcagccaag ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat
    12781 ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg
    12841 taaaagtatt gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg
    12901 ctagttagtg ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta
    12961 aaaataatgt ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct
    13021 gattgaaaaa aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac
    13081 gaactatacg aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt
    13141 ttatcaagac atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg
    13201 tgatgtcatc gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg
    13261 cgttggtgac acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt
    13321 ggctaaaatg tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc
    13381 aattcccgga tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag
    13441 taaagtagcg gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc
    13501 cttgcgtgac gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag
    13561 taaaaaaatc gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc
    13621 tttcatgaag aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa
    13681 actggctaac cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc
    13741 tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga
    13801 agatctagta gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc
    13861 taagacaacc gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt
    13921 agcaaaattc aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc
    13981 agaacgaatt ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt
    14041 gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa
    14101 aaccaaagaa gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga
    14161 cactgtgaca aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc
    14221 tgtcattgaa ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt
    14281 gattcatgac atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag
    14341 gggaaaagaa atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc
    14401 taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg
    14461 catcaatcca gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt
    14521 atgtacagta atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac
    14581 aacagatgcc atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt
    14641 gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa
    14701 agatcgtgcg cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat
    14761 ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt
    14821 ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga
    14881 aatgggtgtc aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga
    14941 agctggtgct acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg
    15001 tgaaggctat taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc
    15061 aacacagcta ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc
    15121 gcttgtctcg taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca
    15181 tttggattaa cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa
    15241 aaaacattta cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt
    15301 gcatgtcgac aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga
    15361 gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa
    15421 aaagatctat aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt
    15481 taggaggctt tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag
    15541 cagcagggtt atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca
    15601 aaagctcatc agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc
    15661 gttcattcaa ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt
    15721 cacgaggaaa tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa
    15781 atattgacca agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa
    15841 aaccagcgat ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg
    15901 atgtcatcga tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt
    15961 atcttgcagg tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg
    16021 gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag
    16081 gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg
    16141 ctccagataa aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct
    16201 tccatgcttc aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat
    16261 caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat
    16321 acacattaaa cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa
    16381 cagtagtaga agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca
    16441 ctgtttatgg attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag
    16501 cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag
    16561 aaactcctga agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg
    16621 ctatggtttg attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga
    16681 taagggaaca actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga
    16741 atcttagtca atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa
    16801 gaaaatattg tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat
    16861 gacaacatca atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt
    16921 gctggcaaat ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa
    16981 attttgatgc gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg
    17041 attgggatgg tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc
    17101 gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag
    17161 atccaaaaag tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat
    17221 atttcagttg gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat
    17281 gtactgattt ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc
    17341 gtgatcatga aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta
    17401 gatgaaatca aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt
    17461 acagtcaacg ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg
    17521 gtttcattca aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa
    17581 aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt
    17641 cgtgctggcg aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc
    17701 caagctttga ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac
    17761 atcactaata aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac
    17821 cgtcataaat acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc
    17881 tatcatcaaa aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat
    17941 gccagagaat tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa
    18001 gctttatcag gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct
    18061 gatctgttga ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt
    18121 cataaacgtc tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa
    18181 ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc
    18241 ggtatcgttg atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat
    18301 accttagaag aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag
    18361 cgaaaaattc cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg
    18421 cgcgatattg atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga
    18481 tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat
    18541 ttttacagca ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc
    18601 tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct
    18661 gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc
    18721 agcggctgtt ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat
    18781 catgatgaac tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga
    18841 aagctatcgc tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt
    18901 accatggtta acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat
    18961 catcgcgctt attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg
    19021 ttctgttgga ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat
    19081 cgttttgtcg atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt
    19141 aggaacgtat caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat
    19201 ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt
    19261 cagtattttg aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat
    19321 cgtcaatgta tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt
    19381 cgtcatggct aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc
    19441 gaaaccaacg aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt
    19501 gccttgatcc ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc
    19561 ggaacttttt ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg
    19621 ggtgctttta gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca
    19681 ccatggatcg ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca
    19741 gtagcaacag tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg
    19801 gcacctgctt taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac
    19861 atcacgcaga atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga
    19921 ccaattttct ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta
    19981 gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat
    20041 cctcaagctg ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg
    20101 atttctggct tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac
    20161 ttttcagctg gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt
    20221 aagtggaatc ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg
    20281 agtatcattg gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt
    20341 gctccatatt tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca
    20401 aaagcaaacg ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg
    20461 gttacgggaa gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga
    20521 aagaaggaat aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg
    20581 gagaagcacc agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg
    20641 cgaaagaagc aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc
    20701 ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag
    20761 tttctgtagg gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa
    20821 ctccatttcg tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat
    20881 tttctggccg taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt
    20941 acggtgaaca ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc
    21001 ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat
    21061 tcgttcgtga tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt
    21121 acgtgggtga accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc
    21181 cattcggaca cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg
    21241 gaaagatcaa cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca
    21301 atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct
    21361 ttaccaattt agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg
    21421 cgcatgcgat tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag
    21481 acgacttgct aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc
    21541 atacgcgaga atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat
    21601 tgcctcaagg atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt
    21661 cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa
    21721 gcaaatgacc aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg
    21781 agtaaaagaa attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga
    21841 aatcgaagat gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt
    21901 agttgggcat gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat
    21961 gcaagggaga ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg
    22021 tgtgatggcc ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa
    22081 cgaatcattt gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga
    22141 aaatcctttg atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca
    22201 agcttatacg cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga
    22261 tcttaaagaa ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat
    22321 ccgaatgtct cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat
    22381 cgtagcagct cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc
    22441 aggtatgcaa gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca
    22501 atcttttaaa gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa
    22561 tgagtgtgca tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag
    22621 atcctttgcg agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc
    22681 aggtacgcgg tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag
    22741 gaacaggaat gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg
    22801 gtgtaaagaa attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc
    22861 gtgatttagt actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc
    22921 aagcctttca ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag
    22981 ctaaagaaaa gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca
    23041 aagatgattt gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg
    23101 aagcagcggc tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga
    23161 ccgttagcga ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt
    23221 tcaatgaaat gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa
    23281 aagctgccct gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga
    23341 cttggctgga cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg
    23401 aatctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
    23461 atgaatattg tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag
    23521 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
    23581 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
    23641 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
    23701 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
    23761 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
    23821 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
    23881 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
    23941 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
    24001 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
    24061 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
    24121 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
    24181 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
    24241 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
    24301 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
    24361 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
    24421 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
    24481 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
    24541 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
    24601 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
    24661 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
    24721 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
    24781 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
    24841 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
    24901 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
    24961 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
    25021 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
    25081 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
    25141 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
    25201 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
    25261 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
    25321 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
    25381 aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc
    25441 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
    25501 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
    25561 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
    25621 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
    25681 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
    25741 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
    25801 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
    25861 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
    25921 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
    25981 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
    26041 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
    26101 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
    26161 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
    26221 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
    26281 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
    26341 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
    26401 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
    26461 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
    26521 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
    26581 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
    26641 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
    26701 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcggtattg cagctaacat
    26761 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
    26821 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
    26881 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
    26941 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
    27001 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
    27061 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
    27121 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
    27181 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
    27241 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
    27301 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
    27361 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
    27421 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
    27481 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
    27541 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
    27601 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
    27661 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
    27721 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
    27781 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
    27841 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
    27901 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
    27961 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
    28021 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
    28081 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
    28141 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
    28201 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
    28261 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
    28321 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
    28381 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
    28441 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
    28501 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
    28561 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
    28621 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
    28681 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
    28741 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
    28801 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
    28861 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
    28921 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
    28981 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
    29041 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
    29101 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
    29161 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
    29221 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
    29281 tgaataattt cttgaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
    29341 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
    29401 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
    29461 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
    29521 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
    29581 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
    29641 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
    29701 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
    29761 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
    29821 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
    29881 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
    29941 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
    30001 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
    30061 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
    30121 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
    30181 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
    30241 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
    30301 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
    30361 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
    30421 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
    30481 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
    30541 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
    30601 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
    30661 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt
    30721 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag
    30781 aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt
    30841 ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta
    30901 ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg
    30961 cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc
    31021 tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt
    31081 taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa
    31141 aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca
    31201 caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac
    31261 acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc
    31321 tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg
    31381 gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca
    31441 ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac
    31501 aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac caagatatgc
    31561 gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta
    31621 agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa
    31681 ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta
    31741 aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt
    31801 ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa
    31861 acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg
    31921 tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa
    31981 aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga
    32041 actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca
    32101 cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg
    32161 taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc
    32221 gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta
    32281 tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca
    32341 gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag
    32401 ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag
    32461 aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa
    32521 aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt
    32581 ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg
    32641 ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc
    32701 agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga
    32761 cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt
    32821 caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg
    32881 atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca
    32941 attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac
    33001 gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat
    33061 tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat
    33121 gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc
    33181 caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg
    33241 atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg
    33301 aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca
    33361 gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt
    33421 atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca
    33481 agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa
    33541 gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac
    33601 gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta
    33661 cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct
    33721 atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt
    33781 aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt
    33841 tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga
    33901 ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa
    33961 aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc
    34021 atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga
    34081 cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag
    34141 aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc
    34201 taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct
    34261 tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc
    34321 tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga
    34381 cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa
    34441 acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat
    34501 cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa
    34561 ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt
    34621 atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg
    34681 atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc
    34741 acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa
    34801 aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac
    34861 cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg
    34921 ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga
    34981 tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact
    35041 cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag
    35101 cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc
    35161 caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa
    35221 acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc
    35281 attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga
    35341 agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat
    35401 tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg
    35461 ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg
    35521 cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa
    35581 aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca
    35641 gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta
    35701 atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac
    35761 gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg
    35821 aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg
    35881 tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta
    35941 tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag
    36001 caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt
    36061 tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat
    36121 atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt
    36181 taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat
    36241 ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc
    36301 caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag
    36361 gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc
    36421 gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg
    36481 aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct tgagacactt
    36541 atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca
    36601 ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag
    36661 ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc
    36721 aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact
    36781 ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt
    36841 ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa
    36901 tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta
    36961 actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg
    37021 gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca
    37081 ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg
    37141 aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag
    37201 acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag
    37261 cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag
    37321 tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa
    37381 actctgatga agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat
    37441 taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa
    37501 aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc
    37561 gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg
    37621 gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat
    37681 ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa
    37741 acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa
    37801 acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg
    37861 tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc
    37921 taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt
    37981 aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa
    38041 aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt
    38101 gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta
    38161 ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt
    38221 cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca
    38281 gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag
    38341 gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat
    38401 tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac
    38461 cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca
    38521 aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata
    38581 aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta
    38641 aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag
    38701 tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt
    38761 tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca
    38821 gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact
    38881 gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt
    38941 gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt
    39001 ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa
    39061 aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa
    39121 gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat
    39181 tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc
    39241 gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg
    39301 gtgacaaaaa cggacacgta gggtttggta caggtaaagc tcaagaagtt cctgaagcaa
    39361 tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa
    39421 caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg
    39481 tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg
    39541 tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa
    39601 cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat
    39661 cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt
    39721 aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct
    39781 tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa
    39841 tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac
    39901 attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc
    39961 aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg
    40021 gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac
    40081 aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact
    40141 atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg
    40201 ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta
    40261 atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag
    40321 aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga
    40381 tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt
    40441 atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct
    40501 ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt
    40561 ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct
    40621 tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa
    40681 aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg
    40741 tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc
    40801 aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg
    40861 agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat
    40921 catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga
    40981 tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt
    41041 gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac
    41101 gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg
    41161 agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc
    41221 attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta
    41281 taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta
    41341 cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta
    41401 tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg
    41461 tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca
    41521 aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat
    41581 cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt
    41641 tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt
    41701 tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa
    41761 cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt
    41821 tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata
    41881 aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac
    41941 cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag
    42001 ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg
    42061 ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag
    42121 caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg
    42181 ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta
    42241 tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca
    42301 ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa
    42361 aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa
    42421 attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga
    42481 attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac
    42541 aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa
    42601 actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact
    42661 gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt
    42721 gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac
    42781 tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg
    42841 aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa
    42901 aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt
    42961 agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa
    43021 cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga
    43081 attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg
    43141 ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg
    43201 tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg
    43261 tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg
    43321 aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat
    43381 agaagcaggt attgcacata tccattctac attcaataac actatcatca tgatcactga
    43441 tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa
    43501 aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga
    43561 acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc
    43621 aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt
    43681 tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt
    43741 gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa
    43801 gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca
    43861 agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc
    43921 gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac
    43981 tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca
    44041 ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta
    44101 ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta
    44161 acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag
    44221 tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg
    44281 gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga
    44341 acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg
    44401 gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg
    44461 atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag
    44521 aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct
    44581 cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg
    44641 agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa
    44701 aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg
    44761 aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc
    44821 gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga
    44881 aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg
    44941 cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg
    45001 aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg
    45061 cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg
    45121 caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga
    45181 ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc
    45241 tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa
    45301 ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt
    45361 gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc
    45421 atggagccaa ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc
    45481 cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga
    45541 cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa
    45601 tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga
    45661 cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa
    45721 gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt
    45781 gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt
    45841 ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat
    45901 atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc
    45961 tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat
    46021 attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt
    46081 gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat
    46141 ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt
    46201 gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag
    46261 acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga
    46321 cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct
    46381 tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac
    46441 gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca
    46501 tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt
    46561 tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat
    46621 tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag
    46681 ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat
    46741 tggatgaacc aacggcagga cttgatcccc agggtagaaa agaaatgatg gagatgtttt
    46801 ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg
    46861 tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg
    46921 agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta
    46981 ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt
    47041 tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg
    47101 aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc
    47161 cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc aaataattgg
    47221 gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat
    47281 ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct
    47341 ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc
    47401 actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt
    47461 atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac
    47521 ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg
    47581 attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag
    47641 cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt
    47701 cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct
    47761 atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg
    47821 cataatagag acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt
    47881 ttgagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa
    47941 gaaaggatgc gagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca
    48001 ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttataaaaaa atcattattc
    48061 cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc
    48121 atacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga
    48181 agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc
    48241 gggaaggaca gatgccggag tacatgcgat tgggcaagta atccattttg attatccaga
    48301 ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc
    48361 tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa
    48421 gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc
    48481 aagttatttt ccttaccctc ttgatttgga aaaaattcag cgggcgcttc ctgatttatt
    48541 agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaagtacg
    48601 tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt
    48661 taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat
    48721 tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa
    48781 tgcagcagga cctacagcac atcctgaagg actttatttg tatgaagtga aatatgaata
    48841 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattactt
    48901 ctcaaaattt tgcaaaattt cggcttattt tcgaaggagc tacttctgga acaccgtgaa
    48961 tcagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccaaa caaaaatagc
    49021 tcgtcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49081 taaggaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggaaaaa
    49141 tagttcctca tattttgccg tttgttgagc taataccgaa ggtcagctga ttgaacaccg
    49201 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaagtaac aatttcaaaa
    49261 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    49321 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    49381 cttaatgagg acagtcggaa caggtttgtc atttttatat ttaaatggca caaatttttc
    49441 taattatttg tcatttagcg c
//
LOCUS       DAYYKC010000010        44219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000010 DAYYKC010000000
VERSION     DAYYKC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.010"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..2776
                     /gene="adhE"
                     /locus_tag="ACHBBW_000615"
                     /gene_synonym="adhC"
     CDS             179..2776
                     /gene="adhE"
                     /locus_tag="ACHBBW_000615"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9152325.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            3005..4111
                     /locus_tag="ACHBBW_000616"
     CDS             3005..4111
                     /locus_tag="ACHBBW_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9152326.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4128..5156
                     /locus_tag="ACHBBW_000617"
     CDS             4128..5156
                     /locus_tag="ACHBBW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9152327.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5162..5641
                     /locus_tag="ACHBBW_000618"
     CDS             5162..5641
                     /locus_tag="ACHBBW_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9152328.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5657..6952
                     /locus_tag="ACHBBW_000619"
     CDS             5657..6952
                     /locus_tag="ACHBBW_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9152329.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6963..7787
                     /locus_tag="ACHBBW_000620"
     CDS             6963..7787
                     /locus_tag="ACHBBW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9152330.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7952..8851
                     /locus_tag="ACHBBW_000621"
     CDS             7952..8851
                     /locus_tag="ACHBBW_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152331.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8977..9846)
                     /locus_tag="ACHBBW_000622"
     CDS             complement(8977..9846)
                     /locus_tag="ACHBBW_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9152332.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9999..11459
                     /gene="rhaB"
                     /locus_tag="ACHBBW_000623"
     CDS             9999..11459
                     /gene="rhaB"
                     /locus_tag="ACHBBW_000623"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9152333.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11456..12712
                     /gene="rhaA"
                     /locus_tag="ACHBBW_000624"
     CDS             11456..12712
                     /gene="rhaA"
                     /locus_tag="ACHBBW_000624"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9152334.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12726..13571
                     /gene="rhaD"
                     /locus_tag="ACHBBW_000625"
     CDS             12726..13571
                     /gene="rhaD"
                     /locus_tag="ACHBBW_000625"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9152335.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13573..13884
                     /gene="rhaM"
                     /locus_tag="ACHBBW_000626"
     CDS             13573..13884
                     /gene="rhaM"
                     /locus_tag="ACHBBW_000626"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9152336.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13966..15117
                     /gene="fucO"
                     /locus_tag="ACHBBW_000627"
     CDS             13966..15117
                     /gene="fucO"
                     /locus_tag="ACHBBW_000627"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9152337.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15244..16002
                     /locus_tag="ACHBBW_000628"
     CDS             15244..16002
                     /locus_tag="ACHBBW_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9152338.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16102..16746
                     /locus_tag="ACHBBW_000629"
     CDS             16102..16746
                     /locus_tag="ACHBBW_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9152339.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16753..17760
                     /locus_tag="ACHBBW_000630"
     CDS             16753..17760
                     /locus_tag="ACHBBW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9152340.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17776..18618
                     /gene="kduI"
                     /locus_tag="ACHBBW_000631"
     CDS             17776..18618
                     /gene="kduI"
                     /locus_tag="ACHBBW_000631"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9152341.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18638..19441
                     /locus_tag="ACHBBW_000632"
     CDS             18638..19441
                     /locus_tag="ACHBBW_000632"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9152342.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19690..20754)
                     /gene="fni"
                     /locus_tag="ACHBBW_000633"
     CDS             complement(19690..20754)
                     /gene="fni"
                     /locus_tag="ACHBBW_000633"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9152343.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20751..21836)
                     /locus_tag="ACHBBW_000634"
     CDS             complement(20751..21836)
                     /locus_tag="ACHBBW_000634"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9152344.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21849..22826)
                     /gene="mvaD"
                     /locus_tag="ACHBBW_000635"
     CDS             complement(21849..22826)
                     /gene="mvaD"
                     /locus_tag="ACHBBW_000635"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9152345.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22819..23763)
                     /gene="mvk"
                     /locus_tag="ACHBBW_000636"
     CDS             complement(22819..23763)
                     /gene="mvk"
                     /locus_tag="ACHBBW_000636"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9152346.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24079..24738
                     /locus_tag="ACHBBW_000637"
     CDS             24079..24738
                     /locus_tag="ACHBBW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9152347.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24823..25728
                     /locus_tag="ACHBBW_000638"
     CDS             24823..25728
                     /locus_tag="ACHBBW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9152348.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25730..26362)
                     /locus_tag="ACHBBW_000639"
     CDS             complement(25730..26362)
                     /locus_tag="ACHBBW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9152349.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26513..26638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26682..27206
                     /gene="infC"
                     /locus_tag="ACHBBW_000640"
     CDS             26682..27206
                     /gene="infC"
                     /locus_tag="ACHBBW_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9152350.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27278..27478
                     /gene="rpmI"
                     /locus_tag="ACHBBW_000641"
     CDS             27278..27478
                     /gene="rpmI"
                     /locus_tag="ACHBBW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9152351.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27531..27890
                     /gene="rplT"
                     /locus_tag="ACHBBW_000642"
     CDS             27531..27890
                     /gene="rplT"
                     /locus_tag="ACHBBW_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9152352.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28142..29479
                     /locus_tag="ACHBBW_000643"
     CDS             28142..29479
                     /locus_tag="ACHBBW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9152353.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29527..31656
                     /locus_tag="ACHBBW_000644"
     CDS             29527..31656
                     /locus_tag="ACHBBW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9152354.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31631..32320
                     /locus_tag="ACHBBW_000645"
     CDS             31631..32320
                     /locus_tag="ACHBBW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152355.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32728..33405)
                     /locus_tag="ACHBBW_000646"
     CDS             complement(32728..33405)
                     /locus_tag="ACHBBW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9152356.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33541..33735)
                     /locus_tag="ACHBBW_000647"
     CDS             complement(33541..33735)
                     /locus_tag="ACHBBW_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152357.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33785..34225)
                     /locus_tag="ACHBBW_000648"
     CDS             complement(33785..34225)
                     /locus_tag="ACHBBW_000648"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9152358.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34229..35074)
                     /locus_tag="ACHBBW_000649"
     CDS             complement(34229..35074)
                     /locus_tag="ACHBBW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9152359.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35223..36641
                     /gene="pepV"
                     /locus_tag="ACHBBW_000650"
     CDS             35223..36641
                     /gene="pepV"
                     /locus_tag="ACHBBW_000650"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9152360.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36698..37645)
                     /locus_tag="ACHBBW_000651"
     CDS             complement(36698..37645)
                     /locus_tag="ACHBBW_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9152361.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37868..38218)
                     /locus_tag="ACHBBW_000652"
     CDS             complement(37868..38218)
                     /locus_tag="ACHBBW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9152362.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38418..39497
                     /gene="pepA"
                     /locus_tag="ACHBBW_000653"
     CDS             38418..39497
                     /gene="pepA"
                     /locus_tag="ACHBBW_000653"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9152363.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39641..39961
                     /locus_tag="ACHBBW_000654"
     CDS             39641..39961
                     /locus_tag="ACHBBW_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9152364.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39983..40447
                     /locus_tag="ACHBBW_000655"
     CDS             39983..40447
                     /locus_tag="ACHBBW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9152365.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40652..41257
                     /gene="ytpR"
                     /locus_tag="ACHBBW_000656"
     CDS             40652..41257
                     /gene="ytpR"
                     /locus_tag="ACHBBW_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9152366.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41296..42585)
                     /locus_tag="ACHBBW_000657"
     CDS             complement(41296..42585)
                     /locus_tag="ACHBBW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9152367.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            43013..43492
                     /gene="rlmH"
                     /locus_tag="ACHBBW_000658"
     CDS             43013..43492
                     /gene="rlmH"
                     /locus_tag="ACHBBW_000658"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9152368.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43695..43820
                     /locus_tag="ACHBBW_000659"
     CDS             43695..43820
                     /locus_tag="ACHBBW_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152369.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            43817..>44219
                     /locus_tag="ACHBBW_000660"
     CDS             43817..>44219
                     /locus_tag="ACHBBW_000660"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9152370.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
BASE COUNT        14054 a         8017 c         9426 g        12722 t
ORIGIN      
        1 aataataata aataacttta tatatttttc tatacaaaca cctgtaaata aagcaatttt
       61 atatttaaaa ataactgtga aaaaaatcac aaaaaagtgt ttacatagaa aaataaatat
      121 tgtatgatat acatgtgaat tatttaacaa ataaccatga cccattagga ggataatgat
      181 ggctaaagta atagaaaaag aaaaaacgaa gacaattgac gtacaagcaa tgattgatga
      241 tttggcagaa aaagcaaatg cagcattgaa agaaatggaa agctttgatc aagaaaaagt
      301 agaccatatc gtgcatgaaa tggcaatggc agctttggat caacatatgc cattagcgaa
      361 gatggctgta gaggaaacag gccgcggtgt ttacgaagat aaagcaatca aaaatatgta
      421 tgcgtccgaa tatatttggc ataatatcaa acatgataaa acagttggtg tgatcaatga
      481 agatgtccaa aaagggttga ttgaaattgc tgaaccagtc ggtgtggttt gcggagtaac
      541 tccaacaacg aatccgactt ccacaacgat tttcaaatca atgatcgcgt tgaaaacaag
      601 aaatccaatc gtttttgctt tccatccaag tgcccaaaaa tcttctgcag aagcagcacg
      661 agtggtacgt gatgctgcta tcgctgccgg agctcctgaa aattgtatcc aatggatcga
      721 acatccttcg atcgaagcga cttctatgct aatgaaccat ccaggtatcg cgatcgtctt
      781 agcgacaggt ggagctggaa tggtcaagtc tgcttactct acaggaaaac cagctttagg
      841 tgtaggtcca ggtaacgtac cagcttatat tgaaaaaaca gcaaaaatta aacgtgcagt
      901 gaacgatttg atcgtttcga aaacatttga taatggaatg atttgtgctt ctgaacaagc
      961 tgtgatcgtg gataaagaaa tctatgcagc agtaaaagca gaattccaag cacaccaagt
     1021 ttatatcgta aaaccagatg aattgcaaaa attagaagat gcagtgatga acgaaggaaa
     1081 atatgcggtc aatccatcaa tcgttggaca ttctgcgatg gaaatcgcaa aattggcagg
     1141 aatcagtgta ccaaaaggaa cgaaaatgct gattgctgaa ttggaaggcg taggcccaga
     1201 ttatccgcta tcaagagaaa aactttctcc agttcttgcc atgatcaaag ctaacaacac
     1261 ggaccatgcg tttgatttgt gtgaaggtat gttgaatctt ggaggattag gacatacagc
     1321 agttatccat tcagaaaacg aagagttgca tgtcaaattc ggtcttcgaa tgaaagcttg
     1381 tcgtatttta gtgaatacac catctgcaga aggcggcatc ggtgatatct ataacgaaat
     1441 gattccgtca ttgactttgg gatgcggttc ttatggtaag aactctgttt ctcgtaacgt
     1501 ctctgctgta aacttaatca atgtcaaaac tgtagcgaaa cggagaaata acatgcaatg
     1561 gttcaaatta ccgccaaaaa tcttctttga aaagaactct ctattgtact tagagaaaat
     1621 ggaaaacgtt gaacgtgtca tgatcgtttg tgaccctggt atggtccaat tcggttactg
     1681 cgatacagtt cgtgaagtat tggcacgacg taaaaatgat gtgaagatcg aagtcttctc
     1741 agatgttgaa ccgaacccat cgacaaatac cgtatacgca ggaacgaaac tgatggcaga
     1801 cttcaagcca gatacagtaa tcgctcttgg aggcggttct gcaatggacg cagctaaggg
     1861 aatgtggatg ttctatgaac acccagacac atctttcttt ggtgcaaaac aaaaattctt
     1921 ggatatccgt aaacggacat ataagatcga caaaccagaa aaaacacaat tcgtatgtat
     1981 cccgacaaca tcagggactg gttctgaagt aacgccattt gcggtcatca ctgacagtga
     2041 aacacatgtg aaatatccgt tagcagatta tgcattgacg ccagatgtcg caatcgtcga
     2101 tcctcaattc gtcatgtcag ttccagcatc tgtcacagca gataccggga tggatgtgtt
     2161 aacacatgca atcgaatcct atgtttcagt catggcttca gattatacac gtggattgag
     2221 cctacaagca atcaagttag tctttgacta tttagaaaaa tcagtaaaaa cacctgatat
     2281 ggaatcacgt gaaaaaatgc acaacgcttc tacaatggct ggtatggcgt ttgctaacgc
     2341 attcctaggt atttgtcact cagtcgcaca taaaatcggc ggggaatacg ggatccctca
     2401 tggacggaca aatgcgattc ttttaccgca tattatccgc tataatgcaa aagatccaca
     2461 aaaacatgcg atgttcccta aatatgacta tttccgtgca gatacagatt acgcagatat
     2521 cgcaaaattc ttaggtctga aaggcgaaac aacagaggaa ttagtcgaag ctttagcaac
     2581 ggctgtttat gaattaggaa aatctgtcgg catcaacatg agtctaaaag ctcaaggagt
     2641 cacacaagaa acactagata caaccgtaga ccgtatggct gaattagctt acgaagacca
     2701 atgtacgaca gctaatccga aagaaccatt gatctctgaa ttgaaacaga tcatcattga
     2761 tgcttacaac ggataagcaa aagaaagcgt agaaaaaaga taagaaaata gatgaaaaag
     2821 aacttggaaa acgttgtttc tccaagttct ttttttaatt tccaagtaac tgaaagttac
     2881 ttttgttatt taacatttag gtcaatttta gacagttttt gttcaaaaag cgatatttat
     2941 agtggacggt attactgcta tattaaggat ataaaatgaa agcgtttaaa aaggtggcgt
     3001 aaagatgatt attaaagaag agatttttgt acctaaagaa ttagtgaaag aaaaaattga
     3061 cctgctcgtt gaaaacctga caacgatcaa agacgataat ggtgaatttt tgttggactt
     3121 tgatggactg aaagtagatg acaaaagctg gacagtgtgg aattggccac aaggtgtcgg
     3181 tctatatggt atttataaaa attaccagat gacaaaaaat cctcgcgctt atcaagtggt
     3241 caatgaatgg tttgaggatc gtatggaaga aggcgctccg cccaaaaacg tcaatacaat
     3301 ggcaccgcta ttgactatgg cttacttgta tgaagacacg aaagattcac gctatttgcc
     3361 ttatttagaa caatgggcag aatgggtcat gaatgacatg cctcggacaa atgaagacgg
     3421 gttacagcat gccacatatg gaccggaaaa taagaatcag ttgtgggacg atacattgat
     3481 gatgacagtg cttcctttgg caaaaatcgg gaaactgttg aatcgcccag aatatttaga
     3541 agaagctcgt taccagttta tgatccatat caaatacttg atggataaac gaacaggatt
     3601 gtggtatcac ggctggacgt ttgaaggaaa tcataactat gccgaagcat tttgggctcg
     3661 tggaaactgc tggttgacga ttgctattcc agagatcatt gaaatcttag aattatcgaa
     3721 agaagatgca ttgcgcaaat tattgatcga aacacttgaa gcacaagtgc gtgcattaaa
     3781 aacttatcag tctgaaagcg gtttatggca tacgttgttg gatgatcctt cttcttacgt
     3841 agaatcttct gcaactgcag gatttgctta cggtattttg aaagcggtcc ataaacgtta
     3901 tttaccacaa gaatataaag aagtcgctta tcgtgctatc caaggattgc ttgaacaaat
     3961 cgatgaaaaa ggagaagttc aaaacgtttc aatcgggaca ggaatggggg acagtttaga
     4021 tttttatcgg aatatcggaa tcacagcaat gccttatgga cagtcgttaa cggtgctttg
     4081 tttgacagaa ttgctcgtct cttattgtta aaagaaagga aacccatatg aaaaaaacat
     4141 cactgctgtt tgcagcaagt tcgcttagcc taggtctgct aggtctaagc ggctgcggaa
     4201 ataattctag tgtggcaact gtcgcagatg cagatgtaaa tgagatagtt accttgcgct
     4261 ttgcttatgc aagcaacagc cagccagtaa ttgatgcaat gaatgaattt gggcgtttag
     4321 taaaagaaaa aacagatggt tccgtacaga tccagtattt cccagatgga caactgggtg
     4381 gagaacgtga actgatcgaa ttgacacagt caggagcagt agatttcaca aaagtaagtg
     4441 cctcagcgtt ggaaagtttc tctaaagatt attcgatttt ttcagttcct tatctattca
     4501 atgatgaaga acatttcttt aaggtgatgg ataatgaaga aataatggag ccaatctacc
     4561 aatcgaccaa aaatttagga tttaccggat tgacctatta tgattctggt cagcgcagct
     4621 tttatatggt agacggtccg atccatacgc cggaagattt gaagggaaag aagatccgtg
     4681 tgatgcaaag cgaaaccgct atcaaaatgg tagagctttt aggcggttcg cccgtaccta
     4741 tgggaagtga tgaagtgtac acgtctcttc aatccaattt gatcaacgga tcagaaaaca
     4801 atgaatttgt cttgtataca gctggacatg gtggtgtggc taaatactat tcctatgatg
     4861 agcatacacg tgtgccagat atcatcatca tgaatgatgc catcaaagaa cagctaactg
     4921 atgagcagga aaaagccatt gaagaagcag cgaaagaatc aacggttttt gaaattgaag
     4981 cttttgcaaa agcaattgaa gaagaaaaag ctttagcaca agaagaacat ggagtccaat
     5041 tcaatgatgt agataataca ccgtttcgag aggcagttgc cccacttcat gatatattca
     5101 aaaacgatcc tacctacaaa gacctttatc aaaagatcca agagcaagga gcgtagctgt
     5161 gatgaaaatg attttaaaca aggtattaga gatcctggga gctgttttgc ttgttgggat
     5221 ggtcgctgtt gttttatggc aagtattttc cagaacggtt ttaggaaatc caaatacggt
     5281 cacggaagag cttgttcgat tcggtcttgt ctggtttgcc atgttgtctt ccgcgtatgt
     5341 tgtgggacaa aaagcacatt tagccgtaac ggttttaagt gaaaaattaa ctggcaaaaa
     5401 aagtaaaatt ttagaagttg ttgttcaaag tttatttctg gtatttgctg cgatgatcat
     5461 ggtgtatggc ggatggaatg ctgtcacatt gacaatgggg cagatctcgc cttctttaag
     5521 tattcctatg ggatatgttt atctgtcagt acctgtatcg ggggtactca tcattatcta
     5581 tagcctgatc aatctaatcg atacattaag caacaagcca gcaattcaag aaaaaatata
     5641 aaaatggagg aataaaatgg caatagaagc cggattagtg ttattttttg tatttgttgt
     5701 gttgcttgta ttagggatgc cgattgctat aagtattgca ttctcatcga tgatgacttt
     5761 attgttagtt ataccgtttg atgtttctgc ttttagttca gctcaaaaaa tggtgggaag
     5821 tatcaatagt ttttcgctga tcgctattcc gtttttcgtt ctctcgggca ttatcatgaa
     5881 taatggaggg atagctaaga aactagtgga ttttgcgatg ttgttcgtcg ggcgcgtgcc
     5941 gggggctttg gctcatacga atgtgttagg gaatgcttta tttggttcca tctctagctc
     6001 ggccattgcc gcttctaccg cgattggcgg agtgttgatc cctcagcaag tggaagaagg
     6061 atatgaccgg aagtttgcga cagcggtcaa tatcgcgtca gctccgacag gtatggtcat
     6121 tcctccaagt actgctttta tcatgtattc acttgttgct ggtggtgctt cgatctctgc
     6181 tttgtttatg ggaggttatc tcgtaggcgc tttgtgggcg ctaggaatca tgctagtagc
     6241 attcatcgtt gctaaaaaga ataaataccc tgttgtcgaa aaaggacaga tggctcatgc
     6301 tggaaaaata ataaaagagg cgattcctag ccttctattg attgtcatca ttatcggtgg
     6361 tatcttgacg gggatattta cagcagtaga agcttcagcg atagctgtca tttactccct
     6421 gttgattgct atgttttact ataaaacggt aaaactaaaa gatataccta atatgttgtt
     6481 tcaatcagta gcaatgtcag ggacgatcat gttcttattg gctacttctt ccatgatgtc
     6541 tttcgccatg gcgtttaccg gtattcctca ggcaattagt tcgttgatta tgggcgtaac
     6601 ggataataaa tttattattc tgttattagt caatcttgtg ttattactgg tgggtatgtt
     6661 tatggatgtg gcgcctgcta tcttgatttt tacaccaata tttttaccaa tcgtcacaag
     6721 tgtaggggtt gatcctgtac attacgggtt gttttcaatc atgaatctgt gtgtaggctc
     6781 catcacgccg ccagtaggta caggactata tgtcggtgca agtgtaggag gcgtaaaagc
     6841 agaggcaatg ctgaaaccgc ttcttccttt ttatggggtt atcttagtcg tcttgttttt
     6901 gattacttac ttcccgcaaa tcgtcatgtg gctacccaat atgctgagtt aggagaatag
     6961 ttatgttgaa cattacaatt agaggacatg atctttccga agtccaaacg atagaagatc
     7021 ttgcagaaaa aacaaaagaa caagggattc atacactgca gctcgcctta ggtctttcgt
     7081 ttcctgaaat accttctggc gcaaatgaga tcaattctgg gatgggaaat tatgtcaaac
     7141 gagttttaga aaagcaagaa gtgaccgtag gaattttaag ttgctatatc aatatgattc
     7201 atccagattt aacgatacga caagagttgc tgacaaagtt cgagcagtat cttcgctatg
     7261 cttcttcctt tggtgcgtcg atggttgctt cggaaactgg atgtgttttg ccggaaatcc
     7321 aatatacgga agaaaatttc acagatgaag catttgctga agctgtgtcc gttatccgtc
     7381 gcttggtaaa agctggagaa aaataccaaa tgatggtggg gatagaacca ggtttgaatc
     7441 atccggtgta ttctttagca agggtagagc agctgatcca agcagtagat tcggactatc
     7501 taggaatcat tttagatccg accaatttga tcacaagtag tgattatcaa gaacaagtcc
     7561 aattggtaga agaagctttt gaacgttttg gtgaaaagat atgtgcggtt catttgaaag
     7621 attttcgtgt cgaacaggaa aaaatcgtgc cggtaaattt gggagacggt gtgatcgagt
     7681 acacaaaaat caaagaaatc atcatgaaga atcgtccgta cctgtatgtg gttcttgaag
     7741 aaacaaaaga tgatgggatt cgttatggaa gaagtcttct tgaataatgt aaagaagacg
     7801 ttggttaact aatatttaga atggaggggg cggggctaga agatgccacg tctcttcttt
     7861 tttataagag tatataaaag cagctctttt tgattacaaa aaacgtggta taataaaatt
     7921 gtgcataagt gaacaaaagg aggtgcggca catgctccaa aagaaaatca gcggtgaaca
     7981 agtcaatacg atggtttctg atcagtatga aatctatcat gtcaaagatg tcgtcagcca
     8041 aaataaaacg atctatcatc atcatgattt ctatgagatc catgctacat taaagggaga
     8101 agcgactttt tatttgaacg gacgacaatt tacgatcaaa gcaggaagtg tgctgctcat
     8161 ccattccaat gatttgcatc gtatcgtccg ccaatcttca gatgtattcg agcgtgtcta
     8221 tatctttgtc accccttctt ttctagaaag tcgttccacg ccatggtcta atctatcggc
     8281 ttgttttcaa ccaatgggag agaagaggag tcgtgtattg caaatagaac aggaagtatt
     8341 gaagcaaaaa ttatcattta tcgaccatcc tcctgataaa aaaatttacg gtgaagacat
     8401 aaaatatgaa caagctctgg tagaatactt gatttttctc aatcaagttg tacaaaaaga
     8461 agagagtgcg caagaacaga atgctgtagt tcctaatgag cgtgtggaac aaatgttaca
     8521 atatatctca aaaaatttat ctcatccgct ttctttagag gaaatggaaa agcagttttt
     8581 tgtcagcaaa taccatatca cacgagaatt taaaaagtac actggattta cgtttcatca
     8641 gtatgtaatg aagaaaaagc tgatctatgc caagcacttg ttgcgagaat accgcagctc
     8701 tagtgctgta tatagtaaaa gtggtttttc ttcttatccg cattttctga aagcattcaa
     8761 gagagagttc ggcataacgc ctaaagaatt tttgaaacgt gatacacaga aacagttggt
     8821 tcattatgaa cattttgaag atgaacaatg agaatgatca ctaaaaaata accgcagtag
     8881 ctgctgttgt ttacatagaa gcagaaactg cggttatttt atttttatct atatatttca
     8941 aaatattttg aaaaaatgac gtacatatta tatgaatcag ctgaatttac gaaagtctct
     9001 cggcgatagt cctgtttttg ctttgaacag gcggctaaag taagcaaaat tctcaatccc
     9061 tatttcttcg gaaatcattc gaaccgtaaa ttctgtatac cgaagaaggt ctttagcgta
     9121 agaaagtttt ttgctgatca aataatcgat tggtggagaa ccgataaatt tggaaaaatc
     9181 tttgctcagc tgatatttat tcaataagaa ttgttgttct aattgatcaa gtgtaatcgt
     9241 ttctttgaaa tgttcgtcta agtattgctt cagtttgtaa acgtaggcgg gaatatcctc
     9301 ttcttcgaaa tctagctgat acttttgcaa aatcagttca ttcagcaact cgtggatagc
     9361 tacagaggac tggaaatcgg tacgggcatt cgtgccttgt tgcatgtgga tcaattctgt
     9421 gatcaaatgg tggatcttgt tttctgatgg atctcctgtt gaatggaagg tgttatggcc
     9481 gtcttttgtg aactcctgat aaaatgattg gacgttctga ccattcaaat ggatccaatc
     9541 catttcccat ggttcttcgc tgatcgtttg ataacgctga tattcttggc agtcaataaa
     9601 aaataagtct ccagctttta gctgttgctg attatcatta tatgtaagcg aacctttgcc
     9661 agacaaagta aactttatca agtaagaagg taaatgtgct cgttcagtat agtaagggat
     9721 tggtgctttg aagtaaccga tttcttggat ataaaaaaat aaattcctgc ttctttgtgt
     9781 aggcgtcaag atcactcttt ttgagtcttc cgaccatgag tggttggctt tttcccagta
     9841 atccatgtaa acactcccaa cttgtcaaga taatgcaatt atataccaaa atagtgtgaa
     9901 gcaaaatata agattctgta ttatgatagc attgtcaata aatgataacg ctttattaat
     9961 aaagtgctaa tcaacaataa agtaaaagag gtggagaaat gaatccaaca tttgttgcca
    10021 tcgatatcgg tgcatcaagc ggacgactaa tgaaaagtac gagggatgca aaaggttctt
    10081 tgatcttgga agaagtacac cggttcaaaa acggctttcg gaacaaagac ggtcacgatc
    10141 gctggaagat cgattatctt atcaaagaga ttttgattgg atttgaaaaa ttaaagcaga
    10201 cagggattga tcgccttttt gcggggatcg atacatgggc agtcgattac tgtctgattg
    10261 atgaacaagg ggatcggata aatgatccga ttgcttatcg ggacaagcgt accaatggag
    10321 cagtagaaca gttttctgaa atctattctc tagataagtt gtatgagcag acagggattc
    10381 agatccagcc tttcaatacc ttgttccaat tgtttgctga agaaaaagaa aatttggcaa
    10441 aagcacataa attgttactg atacctgatt atttagggta tgtctttact ggtaacatgg
    10501 tgacggagaa aactaatgca tctactatgc agctgctgaa tgcagggaca aaagaatggg
    10561 aaatagaact tcttgaaaaa atcggtgtct cgaaggaatt atttccacca ttgacagagc
    10621 cgggaacgat actaggagat ctgcaaaaag aacaattttc aaaatatgat ttgccagatg
    10681 tgacctttat cacagtagca agtcatgata cggcgtcagc tgtattagga acgccaggtc
    10741 aaggagaacg ttggggttat atcagcagtg ggacatggtc gcttttaggg attgaaacaa
    10801 agatcgccaa tgtttctcgc aatgcttttt ctgaaaatta tacgaatgaa tggggtgccc
    10861 ataacacggt gcgttttttg aaaaacatca tggggatgtg gttgatccag gaagttgccc
    10921 gaatgcaaga gtacgagtac agttatgcgg aattggcaga gcttgctgca aaagaaaaac
    10981 cgctgcagca agtgatggaa atcaatgacc ctcgattttt gaatccccca aatatgatag
    11041 aagagattca gcaatactgt tttgaaacag agcagccagt ccctgtctca tcgggagcta
    11101 tcgctcgttg tgtgtatgat aatcttgctc tttgttacgc acaagagctg atcaaagtag
    11161 aacacttaac tggaacgaaa ttggaaaagc tgcacatcgt agggggcgga tcgaataatc
    11221 gtctactgaa tcaattgaca gcagattatg ccggaatcat tgtagaagca ggacctagtg
    11281 aagccacagc aatcgggaat gttgccatgc aaatgctcag tacaggtcag ttttcgaata
    11341 tagaacaagc aagaaatgta atcaaacagt cgtttccatg tgaaactttt tacccagatg
    11401 aaaataagca aaacacgatg gaatggtacg tgaataaaat caaaggagta atcgtatgaa
    11461 cacgattgaa agaaattacg aacaagcaaa agaaaaatat gcaactatcg gtgtagatac
    11521 agatgctgta ctagaaaaga tacaggacat caaaatttct atgcattgct ggcaaggaga
    11581 tgatgtcaaa ggtttcttga caccggatgg cgaattgacc ggtggaatca tggcgacagg
    11641 gaattttcca ggagccgcac gaacacctga agagcttaga caagatctgg agaaggctta
    11701 ttcgttgata ccgggcaaac acaaattgaa ccttcatgct atctatttgg atacagaaga
    11761 atctgtcgat ctaaatgaaa ttgagccgaa acattttgaa aaatgggtag agtgggcaaa
    11821 aaaagaagaa atcggtcttg acttcaatcc gactttcttt tcgcatccaa tgatgaaaga
    11881 cggctttaca ttggctcatc cagataaaga agttcgtgaa tactggatcg aacacgggaa
    11941 aagaagccgc aaaatcgcgg agtactttgg gaaagaaact gggcaaacaa gtatcaataa
    12001 cttttgggta cctgatggga tgaaagataa tccgattgat cgttttaccc cacgggaacg
    12061 tttaatggaa tcactcgatg aaatttttac agaagagtta gatgaacggt atgtacaaga
    12121 tgcagtagaa agcaaactat ttggtttagg cgctgaagct tatacagtcg gttcccatga
    12181 attttatatg ggttacgggt tgactcgtga caaacttgtt tgtctagatg ctggacattt
    12241 tcatccaaca gaagtgattt ccaataaatt atcttcatta gctttattca gcaaaggaat
    12301 catgttgcat gtttcccgtc cagtgcgttg ggattcagac catgtggttt tgatggatga
    12361 tgaattgcag gaaatcgcta aagaattagt ccgtaacgaa ttgttggaaa aaacaaatat
    12421 cggtctggat ttctttgatg caacgatcaa ccgtatcgct gcttgggtca tcggtacgag
    12481 aaatacacaa aaagcactgc tgaaagctat gctggaacca gttgagcgat tgaaagaaat
    12541 ggagctggct tttgacttta cttcaagaat ggcttatacg gaagaattga aagatttccc
    12601 gtatgccgat gtatggaatt atttctgtta caaaaatcaa gttccagttg gtttggattg
    12661 gctggaagaa gtccaggaat atcaaaaaga cgtgttggaa ttaagaaaat aggagtgtgg
    12721 ctatattgaa aaaaatagat attttacaag cgccttatgt gaaagaaatg atcgaaacga
    12781 cgtcgaatct ttatcgcttg ggatgggatg aaagaaatgg cgggaatatt tcttatttac
    12841 taaaagaaga agagatcaca ccgttcttgg atcctgaaaa agtgatccgc cgcatctcga
    12901 tgatttttga tgccagccgt ttagcaggat gctattttat tgtaactggt tcaggaagat
    12961 attttaaaaa tgtagcgagc gaacctgccg agaatcttgg aatcgttcga gttgctgaag
    13021 atggcaaaac actggaactt ttgtgggggc tggaaaacga atgccttcct acaagcgagt
    13081 tgccaagtca tttcatgagt catatcgccc gtttagaagt agacccagat aatcggatcg
    13141 ttatgcattg ccatgctagt catctattag cgatgagttt tacgcatgaa ttagacgaac
    13201 gttcctttag tcgtacgcta tggcaaatgt gtacggaatg cttagttgtt tttccagaag
    13261 gaatttccat cattccgtgg atggttccgg gaacaaatga gattggggaa gcaacagctg
    13321 aaaaaatgaa agaaactcga ttggttttat ggccacaaca tggaatctac gggtcaggaa
    13381 aagacatgga tgaagtcttt ggtttgatcg agacagcaga aaaggcagca gaagtctata
    13441 cgtacgtcaa agcgcaagga ccaatcttgc aaacaatcac ggacgaaaac ctgtggcatt
    13501 tagcagatgc gtttggtgtc actccaaaag caggttattt ggaggaagtt catacgaaag
    13561 caggtgtctg acatggtgaa aaaagctttt tgcatgaaag tttacccaga aaaacaagaa
    13621 gaatacaaaa aaagacatga tgaactttgg ccagaaatgc gtacagcact tcgtgaacac
    13681 ggggtcatcg actattcgat ctatcttgat ccgagtacaa gtttgttatt tggttactta
    13741 gtgatcgaag atgaaaagtt atgggaaaaa atggcaaata catctatcaa tcaaaaatgg
    13801 tggaagtaca tggaagatat catggagaca aatccagatg cctctccagt cagttgggaa
    13861 ttgatgccag tctttgaact gtagcaacaa gcaactgggc aaccggttgc ttggggttct
    13921 ttcgttttta ttgtgaaaaa taatacaaat taggaggaat agaaaatgag taatcgaatg
    13981 attttaaatg aaacatcgta ttttggaaaa ggagcgattg agtcgatcgt ccctgaattt
    14041 cagcagcgag gattcggcaa agcagctgtc attactgaca aaggattgat cgagcacggt
    14101 atagcaacaa aagtaacaga acttctagat aaagcagcaa tcgcttatgc tttatacgac
    14161 gggatcgtac ctaatccaac gatccaaaat gtaaaagatg gagtggcttt tgttaaagag
    14221 gcagaggcag actgtttgat cgcaatcggt ggaggttcgc caatcgatac ggcaaaagcc
    14281 attgggatta ttttgacgaa tccagagttt tcagacgtca ttagtttaga aggtgttgct
    14341 gatacgaaaa atccatgttt acctatatta gctataccaa cgacttctgg aacagcagca
    14401 gaagtaacga tcaactacgt catcacagat gaagtaaatc accgaaaatt tgtttgtgtt
    14461 gatcctcatg atatcccaat cgtcgcgttc atcgacagcg atatgatgat gggaatgccg
    14521 aaaaaattgg ctgcatcaac aggtatggat gctatgacgc atgctattga agggtttatc
    14581 acaaaaggcg cttgggaaat gactgatatg ctgcatttaa aagcaatcga aatcatcggc
    14641 cattctttgg aagcttctgt agatggcgat caaaatgggc gagaaaaaat ggcattagga
    14701 cagtatattg cgggaatggg cttctcaaat gtcggtttag gactggtaca cgggatggct
    14761 cacccgttaa gtgcatggta caacattcct catggcgtag cgtgtgcagc acttttgcca
    14821 actgtcatga aatataacaa agaatatact ggagaaaagt accgtgagat cgctcttgtt
    14881 ctagggatta aaggagcagc tgaaatgtct ttagaagatg taagagaagc ggcttgtggt
    14941 gagatcgatc ggttgagcaa agcggttgga atcccagaaa ccatttctga attaggtgta
    15001 aaagaagcag atattccagc aattgcagaa gatgctttgc gagatgtatg cacaccagga
    15061 aatccaagag aaacgacagt agaagaaatc attgctttat atcaaagttt gatgtaaaaa
    15121 cgaagcggat cttcaacatt aaattgcctg taaagagaaa tggtagttta tatctggata
    15181 tctcttttaa attgatggta tttccgctat aatttttctt ataaagaaat aggagaatat
    15241 tatatggaaa ataaaaagcc ttatggaact gtattgatca aagcctctaa aatactagac
    15301 tatttagcgg aaaaccaaga tgtctctttg cagacgatcg caaagggtgt gggaatgacc
    15361 tcttctacag tcttgaaaat actagatact ttgttgatga tcggctacgt aaataaaaat
    15421 tcagagaaaa actatcgttt aggtgcaaag cttattcgct acgcgaataa aaacattgaa
    15481 caaatcgatt tggtagagtt gactttaccg tttttagaac agctgcagca aaagatcgat
    15541 gagactatcc acttaggggt attggacaac aatgagatct tgtacgtgaa caaactagat
    15601 ccaaaacatc aaacgatccg catgtcttca aaaatcggaa tcacccgacc attatacaat
    15661 tctgcgatgg gcaaagccgt attagcagaa ttcagtgaag agcagtacac tgattatctt
    15721 gaaaagcatc cgttgatccc ttacacagaa tatacgatca ccaatgcatt acggttgaaa
    15781 aaggaaatca aaacggtaaa agaaacccac atcgcatttg acgatgaaga aatcgaacgt
    15841 gatatttttt gtataggagc ctcaatcgtg aaaaacgaac aaatcattgg tgcatttagt
    15901 gtaagtatgc cgaaatatcg tttaacagat gagacaaaag aacaaataat cagtgccctc
    15961 aagcaaacaa aaagcgaaat cgagcaaaga attcaaaaat aatgaaaaaa gcgtttgcaa
    16021 tttttctttt ctgccgtata atatgggtat agatttccaa gttataaaat aaaatttcta
    16081 aataggaaat aggtggagaa gatgaaaaaa attgatattt tatctcgatt gaaaaacgcc
    16141 ggagtgattg cggtggtacg cggaaaaagc aaagaagaag ctttgaatgc ttgccacgca
    16201 atcatcaaag gtggtctaac tggtattgag ttgactttta cagtccctca agcagatcaa
    16261 gtaatcaaag aacttttgtc attctacaaa gaccaaccag aaatagtgat tggcgcaggc
    16321 acagtattag acgctgtcac agctcgcttg gctatcttag ctggcgcaga gtatattgtt
    16381 agcccttcct ttgatcaaga aacggcagag atgtgcaatc tgtatcaaat tccttactta
    16441 ccaggatgta tgactattac cgagatcaaa acagcgctga aaagcggggt agatatcgtc
    16501 aaactatttc ctggaagcgc ttatggacca agtatcattt ctgccttcaa ggcgccgatg
    16561 cctcaagtca atatcatgcc aactggcggt gtgagtctgg acaatatgaa agagtggttc
    16621 gatgctggtg tcgtaacagt cggagtaggt gggaaccttt tagcacccgc agctacagga
    16681 gattttgaca aagtaacgga agtcgcgcaa cagtatgcag caaaaatgaa agaaatcaag
    16741 aggtagctgc ttatgggaaa agtagtgaca ctcggagaaa tcatgttgcg attgtccaca
    16801 gagcagggaa cccgtatcga ttcaagtgaa cgattcgctg cccattatgg cggaggggaa
    16861 gcaaacgtag caatttcttt agctaattac ggacatgaag cgatttttgc tacaaaagta
    16921 cctgataacg cattgggaaa agcagtcaaa aagcacttgc aatgttatgg agtagatacg
    16981 aaacaggtat tatctggtgg atcaagacta ggtacttatt atatggaaac tggtagtggc
    17041 gaacgcgccg cctcagtgat ctatgatcgt gccggatcaa gttttgctga aatggatcat
    17101 ttggaatggt cgctagagga gttattccaa ggaatagata tatttcatgt ttcaggaatc
    17161 accccagctt tgtctaaaaa atggcaaaaa atgacgaaac aggtgataga agctgcaaaa
    17221 aaagctggct gcctgatcag cttcgatatc aactatcgtg gaaaattatg gacacagcaa
    17281 gaagccagcg aagtaatcca tcagctttta cctttagtag atatctgctc agctggaaaa
    17341 atggatgctc tttatttgtt aggtatcgag aaagcgccag aagcagaaga acaacctcta
    17401 atttattatt atcaaaaaat gcaggaaaag ttcccgaata ttcaggtgtt ttattcgacc
    17461 aagcgtaccg ttcattcagc aagtgaaaat gaattgaagg ggacattgtg gatgaaccat
    17521 gaatatgtgg aatcccaagt ccatagaatc accccaatcg tggatcgagt aggtggcgga
    17581 gatgcttttg caggtggcgt gctccatggg attttatcca atatgtctcc gcaagaaatc
    17641 attgattttg ctacggcagc ttctgccttg aagcatacga tccatgggga ctgcaaccaa
    17701 tttagtcaag aagaagtcgc acaattttta gcatgcggct ctggaaaaat cattcgatag
    17761 aaggagtaga agaaaatgca agaaatggaa acacgttata cacacagtcc agaagatatc
    17821 cgtcattatt caacggaaca attgagaaaa gaatttttag tagaaaaggt atttgaacca
    17881 gggaaaatca gtttgactta tacgcacaac gatcgaatga tctttggtgg agtgacacct
    17941 actgaaaaag aattagaaat cacattagac aaagaactag gtgttgatta cttcttagag
    18001 cgtcgtgaac taggtgtgat caatatcggt ggaccaggat tcatcgagat cgatgggcaa
    18061 aaagaagaaa tgaaaaaaca agacggatac tatatcggaa aagaaacacg acatgttgta
    18121 ttttcttctc aagatgctga aaacccagct aaattctata ttagttctgt tccagctcat
    18181 cataaatatc caaatgtaaa aatcagtatc gaccaagtaa aaccaatgga gactggtgaa
    18241 ggcctgacac tgaatgaacg taaaatctac caatacatcc atccaaatgt atgcgaaagc
    18301 tgtcaattac aaatgggtta tacgatttta gaaccaggaa gcgcttggaa tacgatgcct
    18361 tgtcatacac atgaacgtcg tatggaagct tatgtctact ttgattatgc caatgaagat
    18421 acacgtgtgt tccacatgat gggcaaacca gatgaaacaa aacatttagt tgtagacaat
    18481 gaacaagcca ttatttcgcc aagctggtcg atccattcag gtgtaggtac aagcaattac
    18541 tcattcatct gggcaatgtg cggagaaaat atcacctata cagacatgga tatggtgcaa
    18601 atggatcaat taaaataagc ataagggaga gaaaatgatg gaatttagta tggatatgtt
    18661 tcgtttagat ggaaaagtag cattagtcac cggtgctgtc tatggtattg gatttgagat
    18721 tgcacgctct ttagctacag caggagcaaa aatcgtattt aataacttga atcaagaatc
    18781 agttgatgaa ggtatagcgc attataaaga agcaggcatc gatgcaaaag gttatgtctg
    18841 cgatgtgacc gatgaagaag cggttcaagc gatggttcaa cagatcaaag aagatgtagg
    18901 ttcagttgat attctagtca acaatgcagg gatcatcaaa cggacaccga tgatcgaaat
    18961 ggatgcggca gacttccgcc aagtaatcga cgtggattta aatgccccat tcatcgtttc
    19021 aaaagcagtt attccggata tgatcgaaaa aggcggcggt aaaatcatca atatctgctc
    19081 catgatgagt gaattaggac gcgaaacggt cagtgcttat gctgcggcaa aaggcggatt
    19141 aaaaatgctg acgaaaaaca tcgcttctga atacggtcaa tacaatatcc aatgtaatgg
    19201 aattggacca ggttatattg ctacaccgca aactgctcca ttacgcgaaa cacaagaaaa
    19261 cggtgaacgt catccattcg atcaatttat cgttggacgt acgccagcag cacgctgggg
    19321 cgagccaatc gatttggctg gaccatctgt cttcttggct tctagcgcat ctgatttcgt
    19381 taacggccat gtcttgtatg tggacggagg catacttgcc tatatcggaa aacaacctta
    19441 aaagagcttt agtacactgt ttagtggaga ggttgtgaaa tcagcggatt gaacgaatac
    19501 cgaaggacag ctgattaaac atcatttatt cgaaaaaaga ggctgggaca gaagcattta
    19561 actccaagaa ataagaagga attcacgaaa attgcttttc aaatttttgt gaatttcagc
    19621 ttatttccga aggagttgct tttacttccg ccgtttgtac gtgtttagag cgtgagacaa
    19681 aagtgttctt tacttttgtc ccacgctctt ttctgtgcgt cttccatact tcactaagtc
    19741 gatccctctt gcttcgcacc aatccttcac tggtccggaa aagatcagag attgttggtg
    19801 tagggccgcg gtattcgttg cgccgaccat agtataaagc agacgaagct cttcttgcca
    19861 ttgcttcatc agtataatag tttcttctac gccgtgattc atcagatgcg tcagtacggt
    19921 tcctgaagta cccacggcat ttgcgccgag acaaagggct ttaaatatgt cataggcatt
    19981 gcggatacct ccggaggcta ggatttccat agaagtgtct gcttcatttg cttcaagcaa
    20041 tgaagagact gtcgattgcc cccagtcagc taaataactc agttcccgct tggagcgacg
    20101 ggcgttttcg atctgggtga agcttgttcc gcttcgaccg ctgatatcaa ctgtatgaac
    20161 tcctaaagca gctagatcat tgagcgtttc tcttgtcatg ccaaaaccga cttcttttac
    20221 gatgaggggg acgtcaatgg ctgtttgagt ttcttgaata agtactttcc aattggtaaa
    20281 atcacggtct ccctcaggca taaccaactc ttgaggtgca ttcaaatgaa tctgtaaggc
    20341 atctgcgtga aacaatcgga tagcttcttt ggctctttcg acagaagtcc ctgcaccgat
    20401 gttggcaatg attttcccgt gaggatattc ttgtcgcata atcgtataag tatcggctaa
    20461 agaagggact ttcaaggcag cgctgactga accagtggca atcatcaaat ctgcttctct
    20521 tgcgacgatt gcaagatctt ggttgatttt ttttgttttt tcgcttccgc cagtcatcgc
    20581 attgatataa aatgggctgc ttagtgtaaa cccaactgcc tgtgtactta gatcgacatc
    20641 agaaactgct atttgcggca aagggttatg gatgatccgt acgaagtcaa attcgttttt
    20701 ttgtttatca tgaaaggctt tagctagtga aacatgttcg tcttttcgat tcatcggttt
    20761 tcctttcttt gatcgctata gacatgtaac ggcagtggag tgatttctgc tttttcccat
    20821 gcactcatta aaggaagaat gccagatttc tggtcaacaa ttacgattcc gcaatcgccc
    20881 ccacctgcac cagaagattt tgcggctcct tcatactgtt cagctaaatt acacaatttg
    20941 ttcaaagcag gcgtttcgat gacgacccca gtgattgcag ataaatcatg cagtaattgt
    21001 cggttttttc gaatcatctg ttggatcaac gttacattat tttctttaaa ccctttgatc
    21061 atttcgttga cacattctgt actatttttt aagaaaagct gataagccac cattttatct
    21121 tctctcgaac ggtgaacttg atcgaccaaa tcagaagtag aggcagggct acccgtccaa
    21181 ccaatcaata aacgtaaatc ttcaggagca atcaatggct caatggatag acctggccaa
    21241 tccagggcaa gtaactcact gatagaatgc tgagtttctt gttcttggag ccaaggatgg
    21301 tcgaaggttg agaaagcgat ccagccacca taacagctag cagcgatgtc gccgcaggaa
    21361 ccattatctt gaactgctaa attggctagt gctgcaatct tgaaaatctc caactgagat
    21421 aaattcaacg cgtaaaaaac attcaaggct ttgaccgttg caacggtcac ggcaccgcta
    21481 gaacccaatc catatttccg gccatttgaa ctgtctaact cacttgttac tttcagatca
    21541 taaaaggata aaagaatgtt tttttcttgt gcatactttt cagtcaagcg aatagcagca
    21601 aggatataat gaaaaggatt ttcccgaata tctaaaacta attctccgtt gcgtcttgtc
    21661 caacgtacag gcatcccact atattgtgca gattggatac ttccgacttt tcgtgcagat
    21721 tctacagtga ctgtcacgaa ttgatcgact gcagcgataa ctgctggatg gcctgtttca
    21781 acaactgcat attctccggc aatatagagt ttgcctggtg cagatacttc aatcataaaa
    21841 atctcccttt attcaataat cgcaattcct ggaccagcat gtgcagtaat tacttgttct
    21901 ttgctaaata atttggtaaa tgtttcttgt acctcagata aatggctgtt ttccactaaa
    21961 actttgacat tagggccagc atccatcgta aagtagcatg gaatgccttg ctttctcaat
    22021 tgtcgcaccg catccatcgc ttttaagctg tcaggagacc agtaagtgaa tggaggctgg
    22081 gcagctaaag tcgttccatg catttttagt ccgtttcttt ccatcgtttc tccgagaagt
    22141 tggaaatctt ttgttttgat tgcttgtttc aattgatata gatccccttc aacggaatct
    22201 aaccagcctt gataaaaact ggatgtttcg actgtccgac gcatcccatt tctgctggac
    22261 acttcttttt tctggtcgtt gatcaaaacg aaaaccattg ctaaatcgtc ttcgaaagaa
    22321 tcggaaggga ctggcttagc gtaagaactt aagtcgtcat ggcctttttc ccattcgacg
    22381 aaaccaccga aaatacttcg gcaagctgaa ccagacccgc gtcgagcaaa tcttgaaaga
    22441 gagagatcat ctaatccaag cttaagagca gtattgcaag cgcctgctaa agcagctagc
    22501 ccgctggcag aggaggctaa tccagctgcg gtaggaacga aattctgact gattactgaa
    22561 gcttttagtg agaggccggc ttctttacga aaaaggtcaa gaaactgact gacttttttt
    22621 gttgcttttt cgtcttgcaa ggtgccatct agataaaatt catctaccat atagctatca
    22681 gaaaagatga cttctgtctc tgtataaaat gcatccaatg ttaacgaaag gctgttgttc
    22741 attggaagga tgagttcttc atttttctta ccccaatatt tgattagagc aatgttggta
    22801 tatgcgcgtg ctttgccttt aaacataggt atgtactcct aatccttgta tccaagtttc
    22861 agcagcaccc gcatcttcaa gtgcttgact gatttcttgg gcttttgttt ttgtttgtgc
    22921 taatgcaatc atacagccac cgcgtccacc gccagttaat ttggcaccca atgccccggt
    22981 atcttttgct gtttggatca atagattaag aaaatcattg ctgattgtca gcttttccag
    23041 aagtgattgt gattcatcca ttgtctgtgc caacgtttct ggtgaatttt caatgattgc
    23101 ttgttttgct tgcttcgtca agtatcctaa tttttggatc atttgtccag tttcatgagg
    23161 ctgggtttca aagaggtgag cgacatcttt gacggcttct cttgtttgtc ctttgattcc
    23221 tgtatcagcg acaatcaaaa aagcatcgat gttcaaagaa aagtagtcga aaggatgccc
    23281 ttttgtaaaa taaatcggct gcaagctgct agtagcggct gcatcgattc cactaggatt
    23341 accatgggcg attttttccg aaagctggac attttctaat agtatttcac gagacaaagg
    23401 aaatgctaag taatcataaa aagcacgagt gactgctgtg gcgactgcag cgcttgatcc
    23461 cattccacgt tcagcaggaa tcgtgctttc aatggtcagt tgcaagtttt tttggatgtc
    23521 atgctgtttt tttaagtgaa tgaataattt tttgatattt tttaatgcat gaggagcttc
    23581 ggctaaattt cctgaatagt aggaagaaac cagttgatcg tccattgcat ccagctcttc
    23641 aaggaatgcg gtgacttttg ttgcatagaa aggaaaggca atcgctggtt ctccataaac
    23701 aaccgcgtgc tcgcccatca atatgatctt tccgcttgat tctccttggc catagtttgc
    23761 cataatctaa ctccttctca cgtaaaagtg atttttaagc aaaaaataaa gcgcccaaaa
    23821 cgagttgcgc tgcttcacga atttttttgt ccactaaggg tctcaaaaaa cattcctcct
    23881 ttattttact aaaaccatct cagaagtaaa actaagaata cgttttctaa agaaaaaaca
    23941 cttttttctt aagaattttt tgactttttt tcttctttga ctagaaaaaa atacaattga
    24001 cactactaga tattgggggt atatttaaaa aggttggagt taaacacaat atttagtgat
    24061 gaagtgagct ggaggtaaat gagcgtgatc gttttagcag gtacaattgg tgccggaaaa
    24121 tcaagtttaa cagaaatgat ggctgaacac tttgatagtc aggcatttta tgaatcgatt
    24181 gatgataatg aagtcttgcc cttattttat gcaaaccctg aacagtatgc ttttttgctg
    24241 caaatttatt ttttgaataa acgttttgca agcatcaaac aagcaatgaa agatgacaat
    24301 aatgtattgg accgttcgat ttatgaagat tcactgcttt tccatctaaa tgcagatcta
    24361 ggaagagcaa cagaaacaga agttcgtgtg tatgatgaac tgctggaaaa catgatggaa
    24421 gaacttccct atgctgccca taaaaaacat ccggatttat tagttcatat tcgtgtatct
    24481 tttgatacga tgttggagcg tatcgaaaaa cgtggtcgtt cgtatgaaca attgtccttt
    24541 gatccaagtc tatacgatta ttacaaagaa ttgaacagac gttacgatca atggtatgaa
    24601 gaatacaaag aaagtccaaa aatccaaatc gatggagacc gctataattt tgtggaagat
    24661 cctcaagcaa aagaagaagt cttgaaaatg atcgaagaaa aattagcaga gattcgccaa
    24721 accaaagtag ccatttgatt tacagttgct caaataaagg gtaaaccttt atttgagcga
    24781 cttttttctt tatactggaa acaaatgtca ggaggataga gtatgcggga aaaacactat
    24841 tttaatcaag accgtaattt ttggatcaat gtacagcaaa acgatgtgct agctgttaaa
    24901 aacttaaaag aagaatatgg cattagtgac gagatgctga cttattctct agataaaaat
    24961 gaacgggcgc gcgtagagta cgattctttt gatgaagcgt tactgcttgt atcaaatgtt
    25021 ccgcaccagc aaaaagtcga taatcattat gaaactagtc ctatcgcttt tattttaaaa
    25081 gaagacggtt tgtttacatt taccacgccg aataccgagt acgtgattcg actaattcga
    25141 tcgcttttgg aacgaatgcc agacatgagt gtatacagtt tattgtttcg tacattgttt
    25201 ttgatttctg attccttttt tcctttaatc gaggaagtga atagtgaacg tcagcggctt
    25261 aatctgaaat taagagaaaa aacaacgaac aagaatctgc tgcaactttc agatttagag
    25321 atcggtttgg tttacttagt tacaggaaca aaacaaaatg tagtactttt agaacaaatc
    25381 aaggcattgg ctatttatcg caagttgtca gaaaaggaaa aagaagagtt ggacgatgcg
    25441 ctgatcgaag cacgacaagc agttgaaatg acgaacttag cttcgcagat tttagaccag
    25501 ctctctggca cttataataa tttgctgaat aacaacttga atgacacgat gaaatttttg
    25561 acggtctggt cgctgatctt gaccgttcca actattgtga caggattctt cggaatgaat
    25621 ctccagttac cctttaccca ttccgttttc ggatggggaa tcgccttgat catcagtttg
    25681 gtcttatcta tttggatgct tattgcactg tggcggcgga ttcgttgatc tagtctttga
    25741 tctgcaaacc aagggcttgg gcgatcacaa gtgcttgctc ttggttgacg attagtcccg
    25801 ccaaagaatc ttgagataaa gcgattgctg tgaagaaatt agtcgtaaag tctaatccct
    25861 tcagagaagt tcgaaaccag ttcgaaccgg tcaactgatt gtcagacaaa aaaaggtttt
    25921 tccaagtgac ctcataaaat tcgctgtctt ctaattgaca atgatcgaag gagacagctt
    25981 tcaagtttgt actactaaag gaagccatcg aagcaataca atcagtgaaa gtacagtctc
    26041 gaagataact ttcggcaaaa ttcgtacctg ttaatttaca ttgatgaaac acgacttggt
    26101 ggaaactgcc accaatccac tccagattgg aaaaatcaca tttttcaaaa atgacattac
    26161 tgcattcaaa atgacgtaaa actgtttttt gcatcgtaag tttttggata tgactctctc
    26221 ttaataccat atttttggca tcgagatagc tttggtcacc cccgttgatc agaagaccag
    26281 agaagatctc ctcatcttct aaataaaact ctccctttgt aagaagagga agagaaggtg
    26341 gaataggttc gatttttttc atgtgggttc acctcgtttt tctttagtat agcaaagaag
    26401 cgggaatcac tgctagaaag tctgctaatc taatcatttt cagaaaagga agacaaactg
    26461 tatgaaaaac attgacaaaa agattagttt ttgctatact atgatggttg agaaataaaa
    26521 gcagaagcac ccgcttctcg ccttagttga taatgtcact gggctagata gttttcactg
    26581 gttctattag gtgaagtacg cgcgggttct gtaagagaac tcgttttttt attgccgttt
    26641 attcggtaag cttttctttc tctcataata tttggaggtg aatgaccata gcaaaggata
    26701 tgatggttaa cgacggcatt cgtgcacgag agttacgatt gatcggttca gatggtgagc
    26761 aattaggagt taagacaaaa gcagaagcat tgaatattgc agaacaagca aacttggatc
    26821 ttgtgttagt tgcccctggt gcgaaaccac cagttgcgcg aatcatggac tacggtaaat
    26881 accgtttcga acaacaaaag aaagaccgcg aagcgcgtaa aaaacaaaaa gtgatcaatg
    26941 taaaagaagt tcgcttaagt cctacgatcg acttgaatga cttcaataca aaacttcgta
    27001 atgcacgtaa gttcttggaa aagggcgata aagtgaaagc ttctatccgt ttcaaaggcc
    27061 gtgccattac ccataaagaa attggtcaaa aagtcttgaa tcgcttagct gaagaaactg
    27121 cggatattgc aacagtggaa caaaaagcga aaatggacgg acgcagcatg ttcttgacgc
    27181 tggcaccgaa aaacgacagt aagtaatttg aatgactaac tggctgttgg acagccgata
    27241 gtcggataac gaaagatttt gaggaggaaa tatagtcatg ccaaaacaaa aaacacaccg
    27301 cggatcagca aaacgtttca aacgtactgg taaaggcgga ttgaaacgct tccgcgcgtt
    27361 tacaagtcac cgtttccacg gcaaaacaaa aaaacaacgc cgtcaattgc gtaaagcaag
    27421 catggtttct agtggcgact tcaaacgtat tcgccaacaa ctagcaaaaa tgaaataaga
    27481 agtacaaata acagaatctg aagatttatt tttattagga ggaattatac atggcacgtg
    27541 ttaaaggtgg aacagtaact cgtaaacgtc gtaaaagagt gctaaaatta gcaaaaggtt
    27601 actacggttc aaaacacact ttatttaaaa cagcaaaaga acaagtaatg aattcataca
    27661 actacgcata tcgcgatcgt cgtcaaaaga aacgcgactt ccgtaaattg tggattgctc
    27721 gtatcaacgc agcagctcgt atgaatggct tgagctactc aaaattaatg cacggtttga
    27781 aagttgctga aatcgacatc aaccgtaaaa tgttagctga cttggctgtt aacgatgcag
    27841 cagcatttac tgcattggct gaccaagcaa aagaagcttt ggctaaataa gctaaacaat
    27901 tataaagagg aaagaagata acgaaaagtt atgttctttc ctcttttttt atttacaatc
    27961 ctccatcaaa tgaaacagga caataacgta taagtctaaa atagcataat taaggttttt
    28021 taattgaaaa ctgaacattt atggtcaccc tttaatgaaa gcgctaacta cataagttag
    28081 aatgagtttg actgatatat tagtttgctt atcagaaaaa caaaatggac ggaggaataa
    28141 catgagtgat attattattg gtattttgtt agttatctca ttctttttca tggtctggta
    28201 ctgtgtaaaa ggctacaact tgatggtcgg ctttgccatc atggctactg tatggatggg
    28261 attagcgcta gtcggaaaca cgttttcacc aaacccggcg atggaagggc aaggagtaat
    28321 tgatgtgttg acgcatatct atacgacggg gccagctgag tacgccaaat cgatcttggt
    28381 aaatgtcttc tttggtgcct ttttcgggcg tgttctagta gatagcggaa tcgcagctac
    28441 cctgattcga aaggtagttg aacttggagg ggacaagcca agaatcacga tgagcttact
    28501 atgtattgta accgctgtta tattcatgtc gatgacagga atcggaccag tgatttctat
    28561 tgcagttatt gtgttgccaa tcttgatgtc attaggtatt tcagtaccgg ttgcactgtt
    28621 ctcatttatg ggctcgatta tggctggaat ctttgctaat atcgtcaatt tcaaacaata
    28681 tcaaacgatt tatgctggtt tcaatccagc ggctgaaagc tatacttata atgactattt
    28741 ccaaattggt atgattggga tgattgtaag cttagttgtg gtactaacgg tcgctaatat
    28801 atcaatgaac aaaaagaaac gctatgcaat ggcagcaaac gtaccagctg aaggtggcga
    28861 cgcaccaatg atctcatggt tagccgtatt attaccagtt ttaggggtcg ttcttttaga
    28921 tcttccaatc atcttaggtt ttattctagc aggtatctgg gcattgctgt ttactggaaa
    28981 attgcgcgga ggttataaag aaatctgtcg tcagtttgcg aaattattta cagatggcgc
    29041 agtagatgtt gcgccaatgg ttggtttctt gatgacctta gcgatgttca ataattcagc
    29101 tgcgtatgct tctccttatt tttcagctat ttttggagat tggataccgc aatcaccatt
    29161 agtcttggca atcgtatttg ctatattgac accactagga tttttccgcg gtccaatgaa
    29221 tctagtcggt tcaggctcag ctattttagc tgtcgtatta gctgttaatc caacgatgtc
    29281 gccagccttt ttgttcccgt tatttgcaat cacaacgatt gctcctcagc atttagatat
    29341 tacacaatct tgggttgctt ggggattagg ttatacaaaa gtaacttctc gagaatatat
    29401 gaagaaatcc atcccaactg gttggatcat cggagcgatc ttatgcctga tcacgttctt
    29461 attgtacggg aacgcctagt aaacaaaacg tcgataaaaa cgattagatg aatggaggaa
    29521 aagaaaatga aacgttttgt gcgaatggga attgatgttg gcggtacaca tacgaaagca
    29581 gtcgcaatcg ataatgcgac gcacgaaatc atcggaaaat cttcagtgaa aacaacccac
    29641 gatgatgtcc gtggagtagc cgcaggtgtc gttcagtctt ttcaaaattg tttgctggaa
    29701 aataatatca gtccagaaga tgttgtgttt gttgcacatt ctaccacaca agcgactaat
    29761 gcactgattg aaggagacgt agcaaaagtc ggcgtaatcg ggatggcaaa aggtggatta
    29821 gaaggttttt tagcaaaacg acaaactaga ttgaacgata tcgatttagg gaataagaaa
    29881 aaaatagaga ttgtcaatgc attcttacct gtcaaacact taaatgtaga ccgtgtttca
    29941 gaaacgattt cttctttaga aagagaaaga gcggaggtct tggtttcttc tatggcattt
    30001 ggagtagata atggggaacc agaacgagtt gtttatgaag cagcatcggt aaaatcgatc
    30061 cctacaacga tggcttctga catcacaaaa ttatatggat tgacaagaag aactcggaca
    30121 gcagcaatca atgcatctat tttaccaaaa atgctagata cggcaacctc tacagaagat
    30181 tccgtacgag aagcaggggt caatgtttct ttgatgatta tgcgcggaga cggcggagta
    30241 atggaaatca atgaaatgaa aaaacgacct gttttaacca tgctttccgg tccagcagca
    30301 tcagtcatgg gctctttgat gtacttgaga gcgtctaatg gtgtttattt tgaagtaggc
    30361 ggaacaacaa ccaatatcgg ggtcattaaa aatggacgtc cagcaatcga ttattcaatt
    30421 gtaggtggcc acccaacata tatctcttca ttagatgttc gggttctagg tgtggcaggc
    30481 ggttcgatgg tacgggctaa tcagtcagga atcatcgatg tcggtccacg ttctgcacat
    30541 attgccggtc tagattatgc agtatttacg gagacagaaa aaatcaaagg tcctaaggtt
    30601 gaatttttct ctcctaaaga aggagaccct gctgattacg tcaaggtagt gatggaagac
    30661 ggagaagaag tgactattac aaatacatgt gcagccaatg tccttggatt agtgcaagaa
    30721 gaacattttt cttatggaaa tgtcccatct gcacgaaaag ccatccaagc attagctgat
    30781 tattgtcata cgacggttga agacatcgca gaacagatca tggaaaaatc ctatgctaag
    30841 attgaaccgg tgattttaga attggcagac aagtatcact tggagaaaga tcagatttct
    30901 cttgtaggtg tagggggcgg cgctgcctca ttgattactt actttagtaa taaaatgggt
    30961 gtgaaatatt cgattcctga aaacgccgaa gtgatttctt ctattggtgt agcacttgca
    31021 atggtccgag atgttgtaga acgtatcatc ccttcgccaa gtaaagaaga tatacgatcg
    31081 ttgaaaaatg aagcgatgaa taaagcgatt gaatcaggcg ctacaccaga atcgatcgaa
    31141 gtccacgtag aaatcgatcc acagacttca aaagtaacgg ctatcgctac tggttctacg
    31201 gaagtgaaag caaccgatct aaccaaggag atcacaacag aagaagcact agagcttgct
    31261 gcagaggata tgcgattaaa taaaaatgaa gtttgcttat tggaaaatac acctttcttt
    31321 tatgtatgcg gcgaacagaa tcgttcaaaa aatgctggaa gtcttcggat catcgatcaa
    31381 aaaggattta tcaaggtcca acgtggacat gcttcatgta tgaaaacaac tgcagccaat
    31441 tatatgacag ctgtcgaaca actttgggaa gatatggcag tttatcaaac tgaattgatt
    31501 gcccgaccag aattttatct ctgcttagga gcaagagtct ctgattttac agcgacggat
    31561 ctggaacaac tacagctttt gatggatctg gaagtatcta ctatggaacc ggaggaagaa
    31621 gtgatcgtcg ttgccggaaa tatcaaacaa acataagtta gaagagttac taaacgaatt
    31681 acaacagatc cccgaagaaa tttggggatt ctaccagttt cggcgagatt tattttggaa
    31741 aaagattcct ttgtccaagc agaaaattct tatacaacaa tcaatcgatt gtggtatcga
    31801 aacggcatgt agcatcaaaa aaaagtaccc ttttgccgat gtaggagaaa tttgtgaaca
    31861 aatggctgtt ccaatcgttt cctgtgaatc agagcaaata aacgaacgaa tcacttttgc
    31921 aacttatgca gaagatgaag ggattcgatt gatgacggag ccgttagaaa aattaaaatg
    31981 ttcaggacta gcttcgatct ctaaagaaac agcgcaagct ttgattattg gacatgaact
    32041 atttcaccac atcgaagctt ctgttaaggg aatttatacg caaagcgaaa aaattgtgct
    32101 gtggcggttg cctttttata cacatcaatc aaacattcgt gcattgagcg agatagcagc
    32161 gatgagtttt tcaaaagaga tgaatcaaag cgatttttcg ccttatgtat tagaggcagt
    32221 cttactttgg ccatataatg aaattcgtag ccaagggatc ttagaagaaa taaaaaaaat
    32281 agaaaaaaga tgtacggagt atgatttaga ttacaaataa agagactgtg acaaaagtcg
    32341 tgtcacagtc ctttttttcc gaataaacga tgttctcctg tcaactcctc ggtcacaagt
    32401 caaaatattc aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagat
    32461 gaacggcagg ctcacgacct cttggaaaac ggtgttcaaa agcttgcatc tgcggtaata
    32521 agtccagcca agttaaaaac ataagaagct atttttactt ggctgttctt attacttgat
    32581 gcagggcagc tttttcacaa cctctcaata aacggtgtgc aatcagctga ccctcggtat
    32641 tagctcaaaa aaaacggcaa aatatgagga actattttgc cgtttctttc tctaatactc
    32701 aggtcaaaac gctgattcca aaacctctta tttgatgggt ctgtcctgaa tatcttcaaa
    32761 ataatcgggg taattatgaa tcacgtattc ttgctcctcg ataatcatat gtaaatgcat
    32821 cgtgcaaaga aatccgactt catcaaagtt atgatcaatc attgattgaa gcattgtctg
    32881 atgttcatct aatactcgac cccagtctaa ttctgaaatc gtcaaacgta accatctgaa
    32941 acgcgtacta taactttcta gccaatccca aatttctcgt tttccagcaa tttcataaca
    33001 agttcggtga aaatcagtat ccaaatcaaa aaaagctttt ttattttgag cgattactgc
    33061 ctgatcggtt tgttctagta ttttttctag tttagcttga tcttgcttgg tcattttcgc
    33121 actgcattct tgcaggatcg gtttttccaa cttttcccgc gtataacggg ctaatgaagc
    33181 agatcggata tcgatttttg aaacataagt gccggactgt ggaatggttt ttacaagctc
    33241 ttgattgcgc agttggatca gtgcttctcg aatcggtgta cgcccgatag ctagttgttc
    33301 ttctaacact ttatcggaga ttttacttcc aggtgctaac tcggaataaa tgatttgttt
    33361 acgtatagat tcataagctt gctgttgcaa attattgata accatttaac cttcctttct
    33421 atgcagatat aatctattat aacataaatc gcttacaaat cgtgtgggca taaaaacaca
    33481 gtctgcaaaa aaagccctga taggcatgcc tattagagcc tcgaaaagaa aaatattgaa
    33541 tcaatctggt cgtacctctt ggatcggttt gacacgaccg ccgattcttt tagcaaaagc
    33601 ttcggattct tttttgtctt tgaaaatgag cagattgtct aagttttttt cccgagtgcg
    33661 acagccagtt ttatcgatgt aaccgtgcat tactttaacg acatacatgg ggatcacctg
    33721 cttttttctt ttcattatac gaactactcg acacaataca agaaaaatgc ttagacggtc
    33781 tttattaaga atttacatca tccaaagaag cgtacgcttg gaataactga tctaactccg
    33841 gattgacttt gctcagcttc actaatcgtt gcgaatcaga aagtaaaaaa cagtgtttac
    33901 tactgccagt agtttttaat tttttgtctt cagttcccac aatttcgtag ctaaaatcca
    33961 accgtgtccc cgtatacttt tggatagttg gatggatgct cactttttct ccgtaatgga
    34021 tcatttcttt atagctgcaa tttacttcca gaacggggat gatgatccca gattcttcga
    34081 tttttttata gggaaaccct agatgtacca ataaggctac ccgagcctct tcaaaccagc
    34141 ggatataatt gctgtgatga ataatcccca tttgatctgt ttcataataa aaaggttctc
    34201 gcacataacc tgtaaatttc tccataactc atgcctcgtt ttccattgat tttttacaaa
    34261 aataattcat ccatttcgga agtgcttcgc tgatttgtgt cggcaaaaca atatattgtt
    34321 cttttgccag ctggtctcca atgaaactat gaagataaat agctgctgca atcgtttggt
    34381 cattttgagg aaactgggct aaaaatcctg tgatcatccc agctaaagtg tcacccatgc
    34441 cgcccgttgc catagcagca gaacctaaag gattggtgta gaagtgctgg tgaccataaa
    34501 tttttgtctc atgacttttc aaaacgagca cagcaccgat ttcctgttgc ttctttttgt
    34561 tttctttatc ccgttgttct tgtatcggta gagaactcaa ccgttgccac tccatttgat
    34621 gaggtgtaag tacagtttgt tcgggatagg gtagcaaaag atgattagca gcaagtaaac
    34681 ttaaagcaga gccatcaatc accagccatt gttcttttga ttggttttct agtacttgtt
    34741 tgaaacgttc caaactctta gaagaagtac ccaatccggg accaatcaag agtacatctg
    34801 cttgctttat tacggaaaga aactggtcag tatccgtcca aactacaatc attgcttctg
    34861 gcagatgact gtgaagagca ctccgatttt tttcatcagt tactacagtc gtgagacccg
    34921 ctccgctgtg tacggcagct aaagcactca taatgattgc tccgccatat tgttcattgc
    34981 cgccaatcaa aacgatacgt ccaaatgttc ctttatgtga aatttttgga cgaggtgtga
    35041 tgactctttt cactagttgt tcgtttatca gcatctaaaa acctcctttt actcattata
    35101 gcgtatattt tttttagaga aaaacagata agaaaaggaa ttgtcagatt ttttgaacta
    35161 tttttttctg taatttgtgg tatcatgaac acgaagactt aaataagtgg aggattaact
    35221 ttatgacaat tgattggcaa aaagaagtag aagcacgtaa agaagacttg cttgaagact
    35281 tgaaaaatct tttacgtgtc aacagcgagc gcgatgactc aaaagtaaca ccagatgcac
    35341 catttggacc tggacctcgc gacgcattga aacatatgtt agcttatggc gaacgtgatg
    35401 gatttaaagt aaaaaatgtc gataactatg cagggcatat tgatcttggc gaaggagacg
    35461 aaacgctagg gatatttgga cacatggatg tcgttcctgc aggagatggc tgggacacag
    35521 atccttatga accagtaatc aaggatggaa agatctttgc acgcggatca agcgatgaca
    35581 aaggtccaag tatggcagct tactatgcga tgaagatcat taaagaactt gacttgaaac
    35641 tatcaaaaaa agttcgcttc gtagtcggca gcgatgaaga aagcggctgg ggcgatatgg
    35701 cttattattt cgaacatgaa gaagaaccag attttggttt ctcaccagat gctgaattcc
    35761 caatcatcaa tggtgaaaaa ggaaatgttt ctttagcttt acgcttcaaa ggcgacaatg
    35821 caggagatta tgtcttgaaa tcttttgttt ccggacttcg cgaaaatatg gtgccaggaa
    35881 cagcaactgc agcacttcaa gtaccaagtg cagatgcagc tatcgcgatg gaagaagcat
    35941 tttaccaatt catcgaagca aatccagtaa gcggaacgat tgaagcggac aatacgtatg
    36001 taaaaatcga attagttggt aaaggcgcac atggtgcaag cccacaatct ggtatcaatg
    36061 caggttcttt cttggcattg ttcttagata actatgaatt cctaggatct gctaaacaat
    36121 ttatccatgt agcagctgct tatgtccatg aagattttta tggagaaaaa ctaggagtcg
    36181 cttatgaaga tgaaaaaatg ggcaaattga caatgaatgc tggactattt gcttttgaag
    36241 aaaatggtac agaggaagcg aacttcatca atatgaattt ccgttttcca aaaggtgtaa
    36301 cagtagacgg attgcaaagt gacatcgaac aaacagtggg gcaagaaggc gcaacagtta
    36361 ctcgaggtgc tcgcgtaatg gaaccacatt acgtaccaat ggatgatcca ttggtggcta
    36421 cattattgca agtctatgaa gaccacactg gagaaaaagg ctacgaacaa atcatcggtg
    36481 gcggaactta cggacgtttg ttgaaacgtg gcgttgctta tggagcgatg ttcccaggat
    36541 atactgatac aatgcatcaa gcaaacgaat ttatgagtct tgatgacttg ttcagagcaa
    36601 cagcaatcta tgcagatgcc atctatcgtt tggcgaaata attaattaaa aagaggatca
    36661 agaattgcaa attatttttg cagtcttgat ccttttttta ctttctactg tggtattcat
    36721 gataaacatg atatagattc aattcatatt ctttcttagc gttcgtgacg actgtatcca
    36781 aaatcaaccc tgttactaat gaaagaagtg ctaaagtcat caatccagta cttaaaatcg
    36841 cagaagggat gcgtgtaata aagcttgtca gcatatattc gcgaatcaca ggaatccccg
    36901 tgaccaatcc aaataaaaag aagaagatgg tccaaatccc aaagaaaagc aaaggcttat
    36961 aatctttaaa cattttaata atcatcatga tcactttgaa tccgtcagag aatgtattca
    37021 attttgattc gctgccttca ggacgatcac gataatcgat tgggatttca acgaacttga
    37081 attttttatc taaagcatgg atcgtcaatt ccgtttcgat ttgaaaacca gaactcataa
    37141 taggaaaact tttgacaaag atacgattga atcctcgata gccggtcatg acatcaagaa
    37201 tttttgtttt atacattcta gtaattgtat ttctaacaag gttatttcca aaatcgtgga
    37261 aaggtctctt attctcatca aagtaggttc catttgatag gcgatcacca atgaccatat
    37321 ctgcttcacc agaacgtaat ttctcgagca aaccatgtac agcttctgca ggataagtat
    37381 catcgccatc taccattaaa taatagtcgg catcgatatc aaagaacatt tggcggatca
    37441 catttccttt tccttgacga ggttcttttt ttacgattgc tccaccttga acggccaatt
    37501 cgtatgttcg atcggtcgaa ttattatcat agacataaat atctgcttct ggtaattctc
    37561 gtttaaagtc tgcaatgact gttgaaattg ttgcttcctc attgtaacaa ggaatcagaa
    37621 cagcaatttt ttcttgttgt tccatttttc actctcctta actatagaaa acaaaagcta
    37681 aattaaaaat acctcataat caaaggaaat gctagttttt cccgctaaat atcatttatt
    37741 taaaaagaaa atactggaat atatgtttgg aaagctctat ctaaaaaacc acccactaca
    37801 aaagaaggac atgatacctc tcttttgaat agtgggtgcg tattcgttct ctcacttttt
    37861 tgacgtatta atctgttgca cctttttttc ttgaaatttc taatacggtt cctgtatgag
    37921 catctgctaa aaattcataa taaacagttt cgccatcttc tattcgagaa agacctcccc
    37981 gataagcact cgaccgaacg gcgaatttcc taataggttc cttttcaaaa gagatccaag
    38041 aaccttcgat tgcaccttct tttagaaaag atgacttgat attttcaagt acttggtctg
    38101 ctgacaatac ttttttgcga tgataccaaa tcgttgaagc taccccgctc aataatccta
    38161 gaccgattcc aactgctaac ccgcctttaa aatagcttag ttcgctttct tcatacatgt
    38221 ggacatacct ccttcttctc acttgttact ccttgtgctt attttaccac actctcatat
    38281 tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata cgatcggtct
    38341 gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt ttagtctgaa
    38401 atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag ttaacagaat
    38461 tacaaggaac aagcggtttt gaacaagatg ttcgcgctta tatggaaagt catatggaac
    38521 cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttggtctg cgccatcatg
    38581 aagaagcgga tgcaccccgt gtaatggtcg cagcacatat ggatgaagtc ggattcatgc
    38641 tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcggc tggaatcctt
    38701 acgttgtttc tgctcagcgt tttacactta aaacctctac tggtaagaat tatccatgta
    38761 tttcttcttc cgttccccca catttattgc gaggaacaag cggacaaaaa tcagtagaag
    38821 taaccgatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg agttacggtg
    38881 tccttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc aacggaaaga
    38941 atattatcag taaatcatgg gacaaccgct atggctgtac aatggtatta gaagctttag
    39001 aagcgttgca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat gtccaggaag
    39061 aagtaggctt gcgtggttct aaagcttctg tcaataaatt caagccagat ctgttctttg
    39121 cagtagactg ctctgcagca gatgatacag tgacgaaaaa tggcacattc ggacatttag
    39181 gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct cgtttgcgcg
    39241 agtacttgct ggatatcgct gaaacaaaca atatccctta tcaatacttt gtttcaaaag
    39301 gcggtacaga tgcaggtgct gctcatacac aaaacgaagg aatcccaagt acagtgattg
    39361 gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc gattttgaag
    39421 ctgcacgaga aatgttgatt caaacactga aaggtttgga taaatcaaca gtaaatacga
    39481 tcgtatacgg aaaataatgc gatagtgaaa ctataaaatc tggcaaaaga ttgctttttg
    39541 ccagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    39601 ttaattgaaa aatacataaa cagaaagtag aggacaaaga atgattatcc caaatagttt
    39661 agaagaatta gcaggctacg tagaaactgg aaagagtgta tttttcttta ctgccggctg
    39721 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa attgaaaatg acttttctga
    39781 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    39841 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    39901 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa caaaaggctg
    39961 acatctgtta ggaggggtca agatgaatca agaatataag aatattttag taggtatcga
    40021 cggtagtgaa caagcacttg ctgctttcaa aaaagcggta gaagtggctc gccgtaataa
    40081 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataacttta tgggctatgc
    40141 acctattgat caaaatctag tcgatcagca aactgaaaca gccaaggaaa tgatcgaaga
    40201 atgtaaagaa tatgggaaat ctgttgatta cacaaatata gaaggaatta ttgcatatgg
    40261 ttctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgagatcg atctaatcat
    40321 ggttgggcag tcaggtctaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    40381 cgtgatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    40441 taattaagca agtggaactt gtacgacctt tttaatcaat taactaaaac agtaaaggga
    40501 ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaggct
    40561 tcttcggaaa taaagcatga aaaaaatttc atgcagtgaa tgcaattttt tgttaagata
    40621 aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt
    40681 cggagacacc ttgatggtta ttatagccga cgataaagga acagaaaaca ctgttgaacg
    40741 caaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt
    40801 ccatatctcc gatcatctaa caatcgaagg gaacggccaa gtaagcttaa cagatgagga
    40861 attagcgaaa ttgaatcgat tgatcaaaga agcaggattc gaagaacagc ttgaagctga
    40921 tcatgaaccg aagatcgttg tcgggtacgt aaaaacatgc gtcccacatc ctgattctga
    40981 ccatttatcg attactgaga cagaagtaga caatggtaca gtattgcaaa tcgtttgcgg
    41041 tgcacccaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ctatgatgcc
    41101 agatggaatg atgatctggc caggaacttt gcgaggggta gatagctatg gaatgatctg
    41161 ttccgctcgt gaattgcatt tgccaaatgc tccagcaaaa aaaggaatct tagtattgcc
    41221 tgaggatgcc gtagtcggag aagcatttcc aaaataaaga ggttgtgaca agtctttgtc
    41281 acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat
    41341 cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga
    41401 atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt
    41461 gtcatcgatc ttagtaatga cgtcgtattg tttcaatcca gctttttcag ccggtgtagc
    41521 tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg
    41581 ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg
    41641 tgtcactttt ccgtcttttt ccagttgatt gatgatattc actacatcgt tacttggaat
    41701 agcaaatccc attccttcta cgcttacatt agaagtagat tcagaagtac ttgcgatctt
    41761 acttgagttg ataccgataa cttgtccttc gatattaact aaaggaccac cagaatttcc
    41821 agggttgatt gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt
    41881 cgtacttgtc acttgtcggt tcaaggaaga aatgattcct gaagtgacag aatttgcata
    41941 ttcagatcct agtggggaac caatcgcaat cgcaggctca ccaaccttca gagcacttga
    42001 gtcaccaaaa gaggcaactg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc
    42061 tgaataagca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcaagac
    42121 ttcaagacct tgctggccat ctactacgtg attattcgtg actacataag cagtattccc
    42181 gtcttttttg tagatcacgc cgcttccttc actataagct tccaaattac tatcatctga
    42241 gctgctactt tcttcttgct gcccgaatag tccgccaaaa ccgcttgatt ggttttgact
    42301 ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc
    42361 agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat tctgattgcc
    42421 gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt
    42481 cagtagtcct ccaacgatcc ctccaacaag ccccagacct aacttacgcc agaggctatt
    42541 atttttactt tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa
    42601 ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga
    42661 ctgtgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga
    42721 ttttcttttt ccacagatac tgtgtaaaac tacgcggata ggattggaaa aataagggat
    42781 aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct
    42841 aatgaatgtg atgaaataag gtaaaataac actgttttct gtggataaac ctgtggatgt
    42901 tgtgcataac tgttggggaa atctttttaa caaaaatagc caaaataaac atatagacga
    42961 aaaaattgat tataataaac aaagtgagca tgaagaccaa aggagagaaa acatgaatat
    43021 caaaattatc tcagtaggaa aattaaaaga aaaatacctg atccaaggta tcaatgagta
    43081 tgtcaaacgg ctgaatgcct atgcaaaaat cgaattgatc gaagttcctg acgaaaaagc
    43141 cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gggaacggat
    43201 cttagcgaaa atcaaagaac aagaatacgt ctatgcctta gcaattgaag ggaaaaatcc
    43261 tacaagtgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct
    43321 tgtttttgtt atcggcggct ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc
    43381 tcagatctct tttggaaaaa tgacgtatcc acatcaattg atgcgtctga tccttgtcga
    43441 gcaaatctac cgtgccttcc ggatcaatgc gggggctcca taccacaaat aatgatattt
    43501 tttaagagaa aaaattatcc ctattttaaa taatagcaat aggtagtggt agggagaaat
    43561 ttataaataa cttttttatt tgctttggta gccaagtaag ttgcaaaaga aaaatagata
    43621 ctgttaaaaa gataatgtag acatagaaac aaatacgtaa aattttagtt agaataatgg
    43681 caaggagaaa aaacatgata gatgaaaaaa agtgtttaaa aaatctatta gaggatatgg
    43741 atgatattga agaaaactat ctaaatggac tgggaaaaca aatatatttc agatacttaa
    43801 gctatctaaa aatgaaataa gacatagtaa ttttttagct tttttgtttg ctccaaatga
    43861 aacccacaat ttatctgatg agttttttaa aatgtttttg aaaaggtata tagattctaa
    43921 tgatgatacc aaagctgcta tcaattattt tgatgcttta cttaatagct atgaagattt
    43981 aattgtttac agagaaaata agaatattga tattttattg gtatcggaaa aaaataaaat
    44041 agtggtgtgc atcgaaaata aaattctgtc ttcggagtct ggagggcaac ttaataaata
    44101 ccaaatatat atagaacgca attattctaa ctataaaaaa atttttgttt ttttaactcc
    44161 agatggaaat gaacccacaa atcctagttg gggcataatt acttacaaga atatcgttg
//
LOCUS       DAYYKC010000011        42605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000011 DAYYKC010000000
VERSION     DAYYKC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.011"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..1205)
                     /gene="recA"
                     /locus_tag="ACHBBW_000661"
     CDS             complement(156..1205)
                     /gene="recA"
                     /locus_tag="ACHBBW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9152371.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1314..2555)
                     /locus_tag="ACHBBW_000662"
     CDS             complement(1314..2555)
                     /locus_tag="ACHBBW_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9152372.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2795..3373)
                     /gene="pgsA"
                     /locus_tag="ACHBBW_000663"
     CDS             complement(2795..3373)
                     /gene="pgsA"
                     /locus_tag="ACHBBW_000663"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9152373.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3582..4505)
                     /locus_tag="ACHBBW_000664"
     CDS             complement(3582..4505)
                     /locus_tag="ACHBBW_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9152374.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5244..6530)
                     /locus_tag="ACHBBW_000665"
     CDS             complement(5244..6530)
                     /locus_tag="ACHBBW_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9152375.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6523..7791)
                     /locus_tag="ACHBBW_000666"
     CDS             complement(6523..7791)
                     /locus_tag="ACHBBW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9152376.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8043..8504
                     /gene="mscL"
                     /locus_tag="ACHBBW_000667"
     CDS             8043..8504
                     /gene="mscL"
                     /locus_tag="ACHBBW_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9152377.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8563..9009)
                     /locus_tag="ACHBBW_000668"
     CDS             complement(8563..9009)
                     /locus_tag="ACHBBW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152378.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9102..9347)
                     /locus_tag="ACHBBW_000669"
     CDS             complement(9102..9347)
                     /locus_tag="ACHBBW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9152379.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9462..10040)
                     /locus_tag="ACHBBW_000670"
     CDS             complement(9462..10040)
                     /locus_tag="ACHBBW_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9152380.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10117..10647)
                     /locus_tag="ACHBBW_000671"
     CDS             complement(10117..10647)
                     /locus_tag="ACHBBW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9152381.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10651..11511)
                     /gene="rlmB"
                     /locus_tag="ACHBBW_000672"
     CDS             complement(10651..11511)
                     /gene="rlmB"
                     /locus_tag="ACHBBW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9152382.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11504..11899)
                     /locus_tag="ACHBBW_000673"
     CDS             complement(11504..11899)
                     /locus_tag="ACHBBW_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9152383.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11896..13308)
                     /gene="cysS"
                     /locus_tag="ACHBBW_000674"
     CDS             complement(11896..13308)
                     /gene="cysS"
                     /locus_tag="ACHBBW_000674"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9152384.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13305..13841)
                     /gene="epsC"
                     /locus_tag="ACHBBW_000675"
     CDS             complement(13305..13841)
                     /gene="epsC"
                     /locus_tag="ACHBBW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9152385.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13927..14166)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14163..15623)
                     /gene="gltX"
                     /locus_tag="ACHBBW_000676"
     CDS             complement(14163..15623)
                     /gene="gltX"
                     /locus_tag="ACHBBW_000676"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9152386.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15793..16950)
                     /locus_tag="ACHBBW_000677"
     CDS             complement(15793..16950)
                     /locus_tag="ACHBBW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9152387.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16984..18354)
                     /gene="radA"
                     /locus_tag="ACHBBW_000678"
     CDS             complement(16984..18354)
                     /gene="radA"
                     /locus_tag="ACHBBW_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9152388.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18417..18944)
                     /locus_tag="ACHBBW_000679"
     CDS             complement(18417..18944)
                     /locus_tag="ACHBBW_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9152389.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19174..19980
                     /gene="proB"
                     /locus_tag="ACHBBW_000680"
     CDS             19174..19980
                     /gene="proB"
                     /locus_tag="ACHBBW_000680"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9152390.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19977..21224
                     /locus_tag="ACHBBW_000681"
     CDS             19977..21224
                     /locus_tag="ACHBBW_000681"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9152391.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21300..21644
                     /locus_tag="ACHBBW_000682"
     CDS             21300..21644
                     /locus_tag="ACHBBW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152392.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21761..22087)
                     /locus_tag="ACHBBW_000683"
     CDS             complement(21761..22087)
                     /locus_tag="ACHBBW_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152393.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22702..23904)
                     /locus_tag="ACHBBW_000684"
     CDS             complement(22702..23904)
                     /locus_tag="ACHBBW_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9152394.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            24021..24536
                     /locus_tag="ACHBBW_000685"
     CDS             24021..24536
                     /locus_tag="ACHBBW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9152395.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24735..25181)
                     /locus_tag="ACHBBW_000686"
     CDS             complement(24735..25181)
                     /locus_tag="ACHBBW_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9152396.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25228..26706)
                     /gene="msr(C)"
                     /locus_tag="ACHBBW_000687"
     CDS             complement(25228..26706)
                     /gene="msr(C)"
                     /locus_tag="ACHBBW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9152397.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27060..27689)
                     /locus_tag="ACHBBW_000688"
     CDS             complement(27060..27689)
                     /locus_tag="ACHBBW_000688"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9152398.1"
                     /translation="MEQKNDFSTIMTGRRPVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27673..28137)
                     /locus_tag="ACHBBW_000689"
     CDS             complement(27673..28137)
                     /locus_tag="ACHBBW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9152399.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28624..29892)
                     /locus_tag="ACHBBW_000690"
     CDS             complement(28624..29892)
                     /locus_tag="ACHBBW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9152400.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30123..30860)
                     /locus_tag="ACHBBW_000691"
     CDS             complement(30123..30860)
                     /locus_tag="ACHBBW_000691"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9152401.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30927..31496)
                     /locus_tag="ACHBBW_000692"
     CDS             complement(30927..31496)
                     /locus_tag="ACHBBW_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9152402.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31624..32496
                     /locus_tag="ACHBBW_000693"
     CDS             31624..32496
                     /locus_tag="ACHBBW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9152403.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32838..33710
                     /locus_tag="ACHBBW_000694"
     CDS             32838..33710
                     /locus_tag="ACHBBW_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9152404.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33707..35665
                     /gene="nagE"
                     /locus_tag="ACHBBW_000695"
     CDS             33707..35665
                     /gene="nagE"
                     /locus_tag="ACHBBW_000695"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9152405.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35997..36848)
                     /locus_tag="ACHBBW_000696"
     CDS             complement(35997..36848)
                     /locus_tag="ACHBBW_000696"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9152406.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36971..37438
                     /locus_tag="ACHBBW_000697"
     CDS             36971..37438
                     /locus_tag="ACHBBW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9152407.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37701..38183)
                     /locus_tag="ACHBBW_000698"
     CDS             complement(37701..38183)
                     /locus_tag="ACHBBW_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9152408.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38283..39146)
                     /locus_tag="ACHBBW_000699"
     CDS             complement(38283..39146)
                     /locus_tag="ACHBBW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9152409.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39744..40619
                     /locus_tag="ACHBBW_000700"
     CDS             39744..40619
                     /locus_tag="ACHBBW_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9152410.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(40764..40916)
                     /locus_tag="ACHBBW_000701"
                     /pseudo
     CDS             complement(40764..40916)
                     /locus_tag="ACHBBW_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(41184..42515)
                     /locus_tag="ACHBBW_000702"
     CDS             complement(41184..42515)
                     /locus_tag="ACHBBW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9152411.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        12291 a         8962 c         7588 g        13764 t
ORIGIN      
        1 tctattaaat tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata
       61 attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta
      121 ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct
      181 tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta
      241 actagagcag aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt
      301 tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca
      361 atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac
      421 atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc
      481 tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc
      541 aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca
      601 aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg
      661 ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca
      721 tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg
      781 actactacga tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt
      841 tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca
      901 tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt
      961 acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact
     1021 tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag
     1081 ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct
     1141 ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct
     1201 gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt atccttatca
     1261 tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt
     1321 cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca
     1381 gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg
     1441 atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa
     1501 gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat
     1561 tctttgctga ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct
     1621 tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg
     1681 tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc
     1741 ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca
     1801 taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc
     1861 cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca
     1921 tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt
     1981 cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga
     2041 aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc
     2101 ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc
     2161 cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt
     2221 ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta
     2281 ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct
     2341 gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc
     2401 aactcttcta aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct
     2461 agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa
     2521 agttctgttc cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat
     2581 acgctgtatt tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt
     2641 gtcacagctc cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca
     2701 caatatacat aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag
     2761 acggcaggct cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc
     2821 tgtttttgat gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca
     2881 tgatttgatc tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga
     2941 taatagcaac catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt
     3001 ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga
     3061 tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa
     3121 ctagcatttt atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg
     3181 cacgggcaat ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac
     3241 ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag
     3301 gtaaacctac aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat
     3361 ttggtaaatt cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt
     3421 tccaccgata ttatttactt ttccaaatga atttattcaa aggagtattt ttattgaaat
     3481 tgatcttttt gaattaacga agaaggaccg cgacacagat ttgtcacgat ccttcttttt
     3541 tatacctaca agtaaacaga tgcgtaaact aatttatacg atcattctgc gtattgaaga
     3601 gtaaggttca cattttttgg actctgattg ttttcccctg gattaactgg tacggattct
     3661 ccattcgctt tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc atttgttgct
     3721 gtttccggta acacggtact ctgtgtttca tttgctgcca gtgtcccttg ataaacatag
     3781 gcattgtcta ctagcacacc taaccataca cgattgacag cagtaaattc aagggttaca
     3841 ggcttggtag cgtctgtgat atttactgcg atggcactgc cagaatcttc tcctgtagct
     3901 attgccattt tcttttcttc acttgatttt gttgttgatt ctactgtaga agactctttg
     3961 gtctgcgtgc tttgctctga ggaagaggac tcctcgactg atccttctac tttaagcgaa
     4021 gaagctgtgt ctccgattat tggatcagct tgacgatctt gccaagtcat atagcccacg
     4081 acaacgacaa tcgccaaagc aaccaatcct aataaaatga caggaagact tgtccaaaag
     4141 cgacttggat gcgattcttc ctgatgcatt gcttttctag aagtacttac tgtttcaggc
     4201 tgcggacgtt cagggaccga tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa
     4261 tggtctccat cttctcctac agcttcagcg tattgccgaa taaatgcccg cacgtaaaat
     4321 ttaccaggca attggtcaaa atcatcattg tcgattgctt ccaaataacg tttttgaatt
     4381 tttgtaatct gctgtaattc atctaaagat atttttttat tcaggcgtgc ttgccgtaat
     4441 ttttctccga tggtttcaaa agccactcgt tcatcttctc cagtctgttt gattctccga
     4501 agcatctgct gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac
     4561 agctcctttt ttccgaataa acattgttct cctgccaact cctcggtcac aagtcacaat
     4621 atccaataaa tataagaagc tgttttttga acttgttcct tgtgactcgg agttaaacgg
     4681 caggctcacg acctctttct taaacggtgt tcaatcagct gttcctcggt attagctcaa
     4741 aaaccggcaa aatataagga gatatttttc cgtatgtttc tctaatactc aggtcaaaac
     4801 gctgatttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt
     4861 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
     4921 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
     4981 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     5041 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
     5101 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
     5161 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
     5221 acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct
     5281 tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt
     5341 tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata
     5401 tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg
     5461 agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt
     5521 tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga
     5581 tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga
     5641 tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag
     5701 cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat
     5761 ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca
     5821 gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga
     5881 ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt
     5941 ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt
     6001 tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg
     6061 ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt
     6121 cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga
     6181 gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa
     6241 cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct
     6301 tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt
     6361 tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa
     6421 ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt
     6481 acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc
     6541 tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt
     6601 gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag
     6661 ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata
     6721 ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg
     6781 caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat
     6841 accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc
     6901 tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa
     6961 accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata
     7021 agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt
     7081 cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa
     7141 ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat
     7201 ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc
     7261 caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag
     7321 ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag
     7381 acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt
     7441 tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt
     7501 gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt
     7561 tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt
     7621 ctgatccgaa taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt
     7681 gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg
     7741 ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg
     7801 ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact
     7861 tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta
     7921 cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata
     7981 ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat
     8041 tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg
     8101 gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca
     8161 caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac
     8221 tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta
     8281 gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa
     8341 accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg
     8401 cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata
     8461 cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa
     8521 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
     8581 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
     8641 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
     8701 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
     8761 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
     8821 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc
     8881 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
     8941 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
     9001 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
     9061 aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
     9121 aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa
     9181 gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg
     9241 ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg
     9301 atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct
     9361 actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa aaaaagaaat
     9421 gcaataaaaa taaataagaa gctcattaag atttttctaa attattcctc attataaaat
     9481 ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga
     9541 tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa
     9601 attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa
     9661 agatgctgaa ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct
     9721 gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc
     9781 tgcttcctca acagagaata gatttgattg cttataacgc ttttataata acttccaaag
     9841 ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct
     9901 tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa
     9961 agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg
    10021 cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta
    10081 tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc
    10141 gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa
    10201 atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt
    10261 cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc
    10321 tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat
    10381 tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta
    10441 tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc
    10501 cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc
    10561 cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc
    10621 gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg
    10681 atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg
    10741 gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt
    10801 accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat
    10861 atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt
    10921 cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg
    10981 tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc
    11041 tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa
    11101 ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata
    11161 aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt
    11221 ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc
    11281 tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag
    11341 cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg
    11401 atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt
    11461 gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac
    11521 caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat
    11581 gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat
    11641 tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt
    11701 ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa
    11761 cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt
    11821 ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca
    11881 attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa
    11941 taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt
    12001 gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc
    12061 tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc
    12121 caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc
    12181 atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg
    12241 ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc
    12301 agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc
    12361 tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat
    12421 gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt
    12481 ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat
    12541 ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg
    12601 atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt
    12661 tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc
    12721 ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt
    12781 ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg
    12841 gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga
    12901 ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg
    12961 atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg tatcttcttc
    13021 aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt
    13081 acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg
    13141 atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg
    13201 gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg
    13261 gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc
    13321 actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct
    13381 ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc
    13441 ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt
    13501 ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc
    13561 tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt
    13621 ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg
    13681 atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac
    13741 aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg
    13801 tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt
    13861 aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt
    13921 tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc
    13981 tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc
    14041 tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac
    14101 cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt
    14161 gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc
    14221 gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa
    14281 agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt
    14341 ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga
    14401 actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa
    14461 aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta
    14521 acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca
    14581 gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa
    14641 tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt
    14701 tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt
    14761 gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga
    14821 tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca
    14881 ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag
    14941 atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt
    15001 ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt
    15061 tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata
    15121 tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct
    15181 tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga
    15241 ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa
    15301 tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct
    15361 ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa
    15421 ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt
    15481 ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa
    15541 taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca
    15601 tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca
    15661 gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa
    15721 attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc
    15781 tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat
    15841 tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc
    15901 acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac
    15961 catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac
    16021 tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt
    16081 gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt
    16141 tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat
    16201 atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt
    16261 taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat
    16321 gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt
    16381 ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt
    16441 atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc
    16501 ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt
    16561 tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt
    16621 gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc
    16681 gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg
    16741 ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa
    16801 aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg
    16861 accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat
    16921 catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat
    16981 tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat
    17041 tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc
    17101 gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt
    17161 cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga
    17221 agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc
    17281 agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc
    17341 catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa
    17401 catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc
    17461 cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg
    17521 attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga
    17581 gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa
    17641 atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt
    17701 ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt tgagtagttc
    17761 tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt
    17821 catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact
    17881 gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg
    17941 ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc
    18001 gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg
    18061 atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag
    18121 ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt
    18181 tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc
    18241 gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg
    18301 cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc
    18361 gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat
    18421 ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt
    18481 aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg
    18541 aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa
    18601 tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt
    18661 gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc
    18721 acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg
    18781 actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct
    18841 gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt
    18901 tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt tcctccctaa
    18961 ataaactact cctattctaa catatccgag gctgtaaaag actttcttta caaattcttc
    19021 aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa cgactgcaag
    19081 ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag aatagaacga
    19141 aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa
    19201 cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa tatcaatctt
    19261 gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg
    19321 atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt gtcagaacgt
    19381 ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga actgatgaat
    19441 atctataatc aaagattcag tgtatataat caacagacgg cacagatcct cgtgacaaga
    19501 gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga acagcttttg
    19561 attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga tgaattagat
    19621 catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg
    19681 gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa tccgactaca
    19741 aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat tctggcacaa
    19801 gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt aaaagccgct
    19861 tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa tccggcaatt
    19921 atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga cttgaaatga
    19981 ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta gcacttatgc
    20041 ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa aagaatcaac
    20101 aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac aatatcagtg
    20161 aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg gctacagaga
    20221 tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg tggacgaaca
    20281 ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc atcatttacg
    20341 aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca gggaatgccg
    20401 ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc caattattgc
    20461 agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc gatgatactt
    20521 ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg ctgattcctc
    20581 gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct atcattgaaa
    20641 caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct atggcagtga
    20701 acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca gaaacattgc
    20761 tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa ctggtagaac
    20821 acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg gctgctttag
    20881 cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc aaagtggtcg
    20941 attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat tcagaaacga
    21001 tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac gcggcggctg
    21061 tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt ggtgcagaga
    21121 ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat gaattaactt
    21181 ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca
    21241 ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa ggtgtcaata
    21301 tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc gctgcttttc
    21361 tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata gggagtcaac
    21421 gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc
    21481 acattagtta catggttgca ttatgcttat tagcgacaat caattactat tcttttttcg
    21541 atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac ttatcttata
    21601 aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata tccgtacgat
    21661 aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag
    21721 attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg cctggtaaat
    21781 ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa
    21841 gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct gattcagcca
    21901 gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc gcttttctag
    21961 tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa atcaaccgat
    22021 catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga aaatattctt
    22081 ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac ctactgaaaa
    22141 ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc
    22201 ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca caatattcaa
    22261 caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa acgacagtct
    22321 cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct caaaaaacgg
    22381 taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata aaacgctgat
    22441 tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg
    22501 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca aaacgctgat
    22561 tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    22621 aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc
    22681 tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata
    22741 aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg catagctttc
    22801 accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg
    22861 gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat
    22921 cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg
    22981 tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac
    23041 aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa
    23101 aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc
    23161 agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata
    23221 taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc
    23281 ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat
    23341 aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc
    23401 caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat
    23461 catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga
    23521 gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt
    23581 cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt
    23641 ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac
    23701 taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc
    23761 aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc
    23821 tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc
    23881 tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt
    23941 tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata
    24001 agacgagagg taggattttt atgaatactt tagcctatat attattatgc gtgcttttat
    24061 ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc
    24121 acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg
    24181 acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg
    24241 tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa
    24301 aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag
    24361 aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag
    24421 acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc
    24481 aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga
    24541 taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa
    24601 tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact
    24661 atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa
    24721 ataacagtgt tccatcattc attactttga attgctttta gcacacttct tgaaagcggg
    24781 ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata
    24841 taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata
    24901 catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt
    24961 tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca
    25021 tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga
    25081 agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc
    25141 attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa
    25201 tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt
    25261 ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga
    25321 agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc
    25381 catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag
    25441 caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa
    25501 agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc
    25561 tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg
    25621 ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat
    25681 atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc
    25741 taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac
    25801 attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg
    25861 gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa
    25921 tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa
    25981 ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc
    26041 ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc
    26101 tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt
    26161 ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc
    26221 tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc
    26281 aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc
    26341 ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca
    26401 tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag
    26461 gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg
    26521 gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc
    26581 gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat
    26641 agatagttct agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt
    26701 ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag
    26761 cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac
    26821 tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat
    26881 ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt
    26941 attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat
    27001 taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt
    27061 tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc
    27121 gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc
    27181 tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc
    27241 gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt
    27301 tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact
    27361 tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa
    27421 cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg
    27481 aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc
    27541 catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt
    27601 tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cgggtcttct accagtcata
    27661 attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat
    27721 attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc
    27781 gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat
    27841 tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct
    27901 ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg
    27961 atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct
    28021 catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat
    28081 tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat
    28141 ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa
    28201 gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga
    28261 gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac
    28321 caagattatt gattcttatc aaactataga agatagctct tctgcagtag ataattttac
    28381 accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga
    28441 tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga
    28501 gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga
    28561 aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc
    28621 tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc
    28681 tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag
    28741 tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat
    28801 ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg
    28861 attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata
    28921 ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc
    28981 tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata
    29041 ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt
    29101 ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca
    29161 tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt
    29221 atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca
    29281 ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt
    29341 aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc
    29401 aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg
    29461 tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata
    29521 aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa
    29581 tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc
    29641 ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc
    29701 ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc
    29761 aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc
    29821 tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt
    29881 ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac
    29941 taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa
    30001 tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa
    30061 attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg
    30121 gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc
    30181 gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt
    30241 tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt
    30301 tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt
    30361 aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga
    30421 taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc
    30481 atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata
    30541 ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta
    30601 ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact
    30661 tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct
    30721 tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct
    30781 gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact
    30841 ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact
    30901 cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg
    30961 gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct
    31021 gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc
    31081 caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg
    31141 gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa
    31201 cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata
    31261 ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact
    31321 ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta
    31381 ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc
    31441 ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg
    31501 ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac
    31561 atacttaact attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa
    31621 aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc
    31681 cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta
    31741 gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat
    31801 tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg
    31861 aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact
    31921 aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc
    31981 cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta
    32041 cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg
    32101 ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca
    32161 gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg
    32221 gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca
    32281 tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct
    32341 ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca
    32401 ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt
    32461 aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc
    32521 agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct
    32581 taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa
    32641 gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat
    32701 gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt
    32761 ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct
    32821 tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag
    32881 atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa
    32941 atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa
    33001 tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga
    33061 tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt
    33121 tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca
    33181 aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag
    33241 tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg
    33301 caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag
    33361 tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt
    33421 cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac
    33481 gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa
    33541 aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc
    33601 agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa
    33661 gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct
    33721 acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact
    33781 agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca
    33841 agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact
    33901 agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt
    33961 tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc
    34021 agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat
    34081 tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt
    34141 tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt
    34201 gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt
    34261 gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac
    34321 aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat
    34381 tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt
    34441 tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg
    34501 tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt
    34561 tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt
    34621 cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc
    34681 aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc
    34741 gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg
    34801 ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct
    34861 ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa
    34921 aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg
    34981 ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg
    35041 cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt
    35101 acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca
    35161 aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta
    35221 cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga
    35281 aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat
    35341 cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc
    35401 tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt
    35461 cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga
    35521 tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg
    35581 cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat
    35641 cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac
    35701 aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc
    35761 tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta
    35821 tttgactctt cttattactt gatgcagaac agctttttta caacctctga taaacggtgt
    35881 tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc
    35941 acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt tatacattat
    36001 tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact
    36061 ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct
    36121 aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct
    36181 gcatcacttg cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct
    36241 aattcttcat agtcagttag attgccggat aattctaaga tttctggaga gtcactttct
    36301 actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca
    36361 tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca
    36421 ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt
    36481 ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa
    36541 cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt
    36601 tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac
    36661 gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct
    36721 ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc
    36781 atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa
    36841 taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta
    36901 taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg
    36961 atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat
    37021 tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac
    37081 aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac
    37141 aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga
    37201 tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc
    37261 aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca
    37321 gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc
    37381 cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt
    37441 ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc
    37501 gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga
    37561 agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa
    37621 atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta
    37681 ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata
    37741 tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa
    37801 tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct
    37861 tcaggcgatt ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt
    37921 cgggtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg
    37981 ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct
    38041 attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca
    38101 aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt
    38161 tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag ttcatcttac
    38221 ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt
    38281 aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct
    38341 ccccattttg taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc
    38401 cattctgcat agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta
    38461 ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt
    38521 tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct
    38581 ccatcaaatc cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc
    38641 atctcttccc tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact
    38701 ccttcaaatt tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt
    38761 tcgatacgaa attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga
    38821 aaagattttt gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga
    38881 aaagctcttg aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact
    38941 cttgatccat ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc
    39001 attagcgaca tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct
    39061 gtattttcgg ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg
    39121 atcaattggt tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata
    39181 ttatcaaaga aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat
    39241 gttgtatgga tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc
    39301 tgcctatcta tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa
    39361 caaagcggta aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa
    39421 gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc
    39481 atacctcttt tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc
    39541 agataaataa aaaacaaaaa aagttccatc tttaaaacac gctattcttt tactttttct
    39601 cacattctct aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta
    39661 tcaaatatgt aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa
    39721 gtgtttcatg aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt
    39781 tggaacgcgg ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca
    39841 agtacttgtc cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg
    39901 aaaagaccaa catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca
    39961 tggctgctgt cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt
    40021 ccgaactctc gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag
    40081 agattcttat agaacatccg caatttatcg aacgcttcaa agttaagatg ccatttcatg
    40141 caataaatta ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga
    40201 aaaacaaaaa caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag
    40261 cagttgaatc gctcattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag
    40321 tgattgctac tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga
    40381 tagaggactg ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa
    40441 ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac
    40501 gctactccat aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga
    40561 aaaaaataga taaaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc
    40621 aagtaaacga taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa
    40681 cacgaatcat gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt
    40741 gactattagg tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctgggtag
    40801 attttccggc attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc
    40861 actgcatctg tatattggat aaaattctga caacaacttt caggtgtgtc gaaccactct
    40921 aatctttaaa gataaatatt aaaataacga ttaataatat taacagtctt tatttttcac
    40981 caccaaaaac ctatctttgc atatactaaa tagacgtata tacacagata ggtttgtctt
    41041 gtgaaaaata atgattatcc ttaccaaaaa atgtttgttt ttctacatgt tcgtacttat
    41101 caaaaaatat gatccttatt gaaattactg gctatttata ttccaacgac ttcttgtgtt
    41161 aatttttgtt atctcatagt aaattatgtt tcattttcac ttaatttagt tgtcacgtgt
    41221 tcgtcaattc gtaaaaacgg taaatatata aacacacttg cggctataat taatatttgt
    41281 aaaacagctg ctcgccagtc tccggctgtt gctaaaaatc ccgatataac tggtggcgta
    41341 gtccaaggaa tttgcactac tacatgatta attaatccca cagcagttgc aaaatatgct
    41401 actgtcaaag aaaaaatcgg tgcaagcaca aagggaataa tcagactgat attaaataca
    41461 ataggcaatc caaaaataat aggttcgctt atattgaata tagcgggagt taccgataat
    41521 ttagcaaagt ccttataatc ttttctacga ctgaagataa atattgcaat caataaagca
    41581 atcgtatttc ccgatccacc cattaccgca aaggtatctt taaaggccat attgataata
    41641 tgtggaattt gctctttgtt tgcataagct gccatatttt cttgcatatt ctgaatcaaa
    41701 aacggatcta acaatgaacc tgatattact gcttgatgga taccaaatcc aaaaaataga
    41761 ttagcaatag atgtaattaa caaaaatcct ggtaaaccag ttgtaatttt acttaatggc
    41821 tctgtaacta tcgtggtaat tagcttattt atatccatat taaacaattg aatgattata
    41881 aatgacaata aagcaaacga ggtaatcgta ataatgattg ggattaatac attaaaagac
    41941 tttaaaacag cgggaggaat atttccggat agctgaatct gtagtttctt attattagaa
    42001 agtttaataa ataattctgt agccgctaat ccaacaaata tacctacaaa cattcctgaa
    42061 gctcctgtat aggatatagg tatcacaccc gtcacttcaa cagctttatc cgcattttca
    42121 ggagtaaaca tcatctgcat aggtgttaat acaacaaatg tcgaaatagc aactaatacc
    42181 gctgcaatat gatttgtata atttctgctt gcacacatat gataagatac tgctgctaca
    42241 actaataatg tagttatgct taatgtacca ttggcaattg aagtaccaat ttgttgtaac
    42301 gtggtcaatg tactagtatc gacaatttcc cccatgaatc ctgttggttc taagatcact
    42361 gaattaataa gtgtcacaaa tcctgctaac atcataaatg gcataaatgt agcaaacgca
    42421 tctcgcaaag ttcttaaatg gatctgattc cccaacttag tcgcaactat accaaatttt
    42481 tcaaatacaa tgtccttcct gctacttttg cccatatttt ttctccttta tgcttattgt
    42541 aatcgattac tttttccacc caaataatac atcacttatc aaaaaaagta aatactttta
    42601 attaa
//
LOCUS       DAYYKC010000012        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000012 DAYYKC010000000
VERSION     DAYYKC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.012"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBBW_000703"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBBW_000703"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9152412.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBBW_000704"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBBW_000704"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9152413.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBBW_000705"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBBW_000705"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9152414.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHBBW_000706"
     CDS             2179..2499
                     /locus_tag="ACHBBW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9152415.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="ACHBBW_000707"
     CDS             complement(2611..3480)
                     /locus_tag="ACHBBW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9152416.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHBBW_000708"
     CDS             complement(3576..4880)
                     /locus_tag="ACHBBW_000708"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9152417.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHBBW_000709"
     CDS             complement(4877..5386)
                     /locus_tag="ACHBBW_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152418.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHBBW_000710"
     CDS             complement(5561..7696)
                     /locus_tag="ACHBBW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9152419.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHBBW_000711"
     CDS             complement(7779..9434)
                     /locus_tag="ACHBBW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9152420.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHBBW_000712"
     CDS             complement(9436..9750)
                     /locus_tag="ACHBBW_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9152421.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHBBW_000713"
     CDS             9920..10180
                     /locus_tag="ACHBBW_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9152422.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHBBW_000714"
     CDS             10194..10508
                     /locus_tag="ACHBBW_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9152423.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHBBW_000715"
     CDS             complement(11238..11792)
                     /locus_tag="ACHBBW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9152424.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHBBW_000716"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHBBW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHBBW_000717"
     CDS             complement(14153..14869)
                     /locus_tag="ACHBBW_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9152425.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBBW_000718"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBBW_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9152426.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBBW_000719"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBBW_000719"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9152427.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHBBW_000720"
     CDS             complement(18339..19799)
                     /locus_tag="ACHBBW_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152428.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHBBW_000721"
     CDS             complement(19789..21084)
                     /locus_tag="ACHBBW_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9152429.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHBBW_000722"
     CDS             complement(21416..22093)
                     /locus_tag="ACHBBW_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9152430.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHBBW_000723"
     CDS             complement(22123..23214)
                     /locus_tag="ACHBBW_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9152431.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHBBW_000724"
     CDS             complement(23315..23749)
                     /locus_tag="ACHBBW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9152432.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHBBW_000725"
     CDS             complement(24333..25790)
                     /locus_tag="ACHBBW_000725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9152433.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHBBW_000726"
     CDS             complement(25787..27682)
                     /locus_tag="ACHBBW_000726"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9152434.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHBBW_000727"
     CDS             complement(27740..29515)
                     /locus_tag="ACHBBW_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9152435.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHBBW_000728"
     CDS             complement(29565..31061)
                     /locus_tag="ACHBBW_000728"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9152436.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHBBW_000729"
     CDS             complement(31255..32877)
                     /locus_tag="ACHBBW_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9152437.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHBBW_000730"
     CDS             complement(33205..34518)
                     /locus_tag="ACHBBW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9152438.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHBBW_000731"
     CDS             complement(34763..34987)
                     /locus_tag="ACHBBW_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152439.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHBBW_000732"
     CDS             complement(35007..35429)
                     /locus_tag="ACHBBW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9152440.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHBBW_000733"
     CDS             complement(35571..36281)
                     /locus_tag="ACHBBW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9152441.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHBBW_000734"
     CDS             complement(36299..36796)
                     /locus_tag="ACHBBW_000734"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152442.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHBBW_000735"
     CDS             complement(36833..37285)
                     /locus_tag="ACHBBW_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9152443.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHBBW_000736"
     CDS             complement(37420..38025)
                     /locus_tag="ACHBBW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9152444.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHBBW_000737"
     CDS             complement(38475..39095)
                     /locus_tag="ACHBBW_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9152445.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHBBW_000738"
     CDS             complement(39097..39966)
                     /locus_tag="ACHBBW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9152446.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHBBW_000739"
     CDS             complement(39989..40396)
                     /locus_tag="ACHBBW_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9152447.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHBBW_000740"
     CDS             complement(40415..41704)
                     /locus_tag="ACHBBW_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9152448.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAYYKC010000013        39762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000013 DAYYKC010000000
VERSION     DAYYKC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.013"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..608
                     /locus_tag="ACHBBW_000741"
     CDS             24..608
                     /locus_tag="ACHBBW_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152449.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            619..864
                     /locus_tag="ACHBBW_000742"
     CDS             619..864
                     /locus_tag="ACHBBW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152450.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            870..1592
                     /locus_tag="ACHBBW_000743"
     CDS             870..1592
                     /locus_tag="ACHBBW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152451.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1723..2466
                     /locus_tag="ACHBBW_000744"
     CDS             1723..2466
                     /locus_tag="ACHBBW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152452.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2472..2837
                     /locus_tag="ACHBBW_000745"
     CDS             2472..2837
                     /locus_tag="ACHBBW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152453.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2853..3383
                     /locus_tag="ACHBBW_000746"
     CDS             2853..3383
                     /locus_tag="ACHBBW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152454.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3545..3775
                     /locus_tag="ACHBBW_000747"
     CDS             3545..3775
                     /locus_tag="ACHBBW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152455.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(3979..4458)
                     /locus_tag="ACHBBW_000748"
     CDS             complement(3979..4458)
                     /locus_tag="ACHBBW_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152456.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            4542..5915
                     /locus_tag="ACHBBW_000749"
     CDS             4542..5915
                     /locus_tag="ACHBBW_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9152457.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            5938..6465
                     /locus_tag="ACHBBW_000750"
     CDS             5938..6465
                     /locus_tag="ACHBBW_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152458.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            6452..7015
                     /locus_tag="ACHBBW_000751"
     CDS             6452..7015
                     /locus_tag="ACHBBW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152459.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            7438..8175
                     /locus_tag="ACHBBW_000752"
     CDS             7438..8175
                     /locus_tag="ACHBBW_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152460.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8192..8608)
                     /locus_tag="ACHBBW_000753"
     CDS             complement(8192..8608)
                     /locus_tag="ACHBBW_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152461.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9000..10247
                     /locus_tag="ACHBBW_000754"
     CDS             9000..10247
                     /locus_tag="ACHBBW_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9152462.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10402..10527
                     /locus_tag="ACHBBW_000755"
     CDS             10402..10527
                     /locus_tag="ACHBBW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152463.1"
                     /translation="MINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTRDEA"
     gene            10517..12907
                     /locus_tag="ACHBBW_000756"
     CDS             10517..12907
                     /locus_tag="ACHBBW_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9152464.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            12956..14791
                     /locus_tag="ACHBBW_000757"
     CDS             12956..14791
                     /locus_tag="ACHBBW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9152465.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            14866..15723
                     /locus_tag="ACHBBW_000758"
     CDS             14866..15723
                     /locus_tag="ACHBBW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9152466.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            15713..16822
                     /locus_tag="ACHBBW_000759"
     CDS             15713..16822
                     /locus_tag="ACHBBW_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9152467.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            17161..17961
                     /locus_tag="ACHBBW_000760"
     CDS             17161..17961
                     /locus_tag="ACHBBW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152468.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18067..18855
                     /locus_tag="ACHBBW_000761"
     CDS             18067..18855
                     /locus_tag="ACHBBW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152469.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19087..19956
                     /locus_tag="ACHBBW_000762"
     CDS             19087..19956
                     /locus_tag="ACHBBW_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9152470.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            19976..20323
                     /locus_tag="ACHBBW_000763"
     CDS             19976..20323
                     /locus_tag="ACHBBW_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152471.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20336..21598
                     /locus_tag="ACHBBW_000764"
     CDS             20336..21598
                     /locus_tag="ACHBBW_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152472.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            21678..23054
                     /locus_tag="ACHBBW_000765"
     CDS             21678..23054
                     /locus_tag="ACHBBW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9152473.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            23051..23680
                     /locus_tag="ACHBBW_000766"
     CDS             23051..23680
                     /locus_tag="ACHBBW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152474.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            23750..24178
                     /locus_tag="ACHBBW_000767"
     CDS             23750..24178
                     /locus_tag="ACHBBW_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152475.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            24737..25336
                     /locus_tag="ACHBBW_000768"
     CDS             24737..25336
                     /locus_tag="ACHBBW_000768"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9152476.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            25352..25828
                     /locus_tag="ACHBBW_000769"
     CDS             25352..25828
                     /locus_tag="ACHBBW_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152477.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            25845..26747
                     /locus_tag="ACHBBW_000770"
     CDS             25845..26747
                     /locus_tag="ACHBBW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152478.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            26820..27014
                     /locus_tag="ACHBBW_000771"
     CDS             26820..27014
                     /locus_tag="ACHBBW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152479.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            27119..27547
                     /locus_tag="ACHBBW_000772"
     CDS             27119..27547
                     /locus_tag="ACHBBW_000772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152480.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            27783..28370
                     /locus_tag="ACHBBW_000773"
     CDS             27783..28370
                     /locus_tag="ACHBBW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152481.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            28370..29269
                     /locus_tag="ACHBBW_000774"
     CDS             28370..29269
                     /locus_tag="ACHBBW_000774"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFM9152482.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            29269..30183
                     /locus_tag="ACHBBW_000775"
     CDS             29269..30183
                     /locus_tag="ACHBBW_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152483.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            30235..30450
                     /locus_tag="ACHBBW_000776"
     CDS             30235..30450
                     /locus_tag="ACHBBW_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152484.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            30640..30894
                     /locus_tag="ACHBBW_000777"
     CDS             30640..30894
                     /locus_tag="ACHBBW_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152485.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            31073..32122
                     /locus_tag="ACHBBW_000778"
     CDS             31073..32122
                     /locus_tag="ACHBBW_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9152486.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            32299..32529
                     /locus_tag="ACHBBW_000779"
     CDS             32299..32529
                     /locus_tag="ACHBBW_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152487.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            32532..32789
                     /locus_tag="ACHBBW_000780"
     CDS             32532..32789
                     /locus_tag="ACHBBW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152488.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            33006..33881
                     /locus_tag="ACHBBW_000781"
     CDS             33006..33881
                     /locus_tag="ACHBBW_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152489.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            33891..34685
                     /locus_tag="ACHBBW_000782"
     CDS             33891..34685
                     /locus_tag="ACHBBW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152490.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            34744..35832
                     /locus_tag="ACHBBW_000783"
     CDS             34744..35832
                     /locus_tag="ACHBBW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152491.1"
                     /translation="MQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYRQNKQTGIHFL
                     GYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSRIMRNYPLPSN
                     TYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYANPKSQSMFAD
                     YGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKT
                     DPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYE
                     QKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKG
                     FENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            35865..36185
                     /locus_tag="ACHBBW_000784"
     CDS             35865..36185
                     /locus_tag="ACHBBW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152492.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            36440..37129
                     /locus_tag="ACHBBW_000785"
     CDS             36440..37129
                     /locus_tag="ACHBBW_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFM9152493.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            37166..37333
                     /locus_tag="ACHBBW_000786"
     CDS             37166..37333
                     /locus_tag="ACHBBW_000786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152494.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            37353..38981
                     /locus_tag="ACHBBW_000787"
     CDS             37353..38981
                     /locus_tag="ACHBBW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152495.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            39032..39403
                     /locus_tag="ACHBBW_000788"
     CDS             39032..39403
                     /locus_tag="ACHBBW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152496.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            39488..39688
                     /locus_tag="ACHBBW_000789"
     CDS             39488..39688
                     /locus_tag="ACHBBW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152497.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
BASE COUNT        15086 a         5357 c         8001 g        11318 t
ORIGIN      
        1 atgtagagtg agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc
       61 ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat
      121 tttatctaat tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt
      181 tcgatataga aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag
      241 attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac
      301 aagatgctga tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat
      361 tttatcttat gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa
      421 aaacaatcaa ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat
      481 ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg
      541 aacttaggga atatgtgcag aacccatata attttcaata tttagcagga agaataaatt
      601 tagtttagga gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt
      661 gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc
      721 attcaaaaaa atccacaata cctattattt agtctttatg cttggattgt acaaatggtt
      781 tgggtttcca tattgatgct gttgttttat tggatagtac gggttattct tttattgtta
      841 aacaaaagaa aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt
      901 tatcgacagc agcattaata acccttacag catgtggtac aactaatagt gctaataata
      961 acaaagacac aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa
     1021 caacaatgtc ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta
     1081 ataaaggtca aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg
     1141 aaaattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg
     1201 attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta
     1261 gttatgctcc aacattaatg catgatatta ccataaataa accagcttac gagtattacg
     1321 cagttgctgt agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg
     1381 ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta
     1441 acggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg
     1501 aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa caatcagagc
     1561 ctgttatgga cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt
     1621 aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga
     1681 aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca
     1741 aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa
     1801 ggaatttata aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta
     1861 aaggcaacaa gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca
     1921 agtgaagaaa tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa
     1981 aaacatctac tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa
     2041 aaattaagaa ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc
     2101 ttaaatgact acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac
     2161 acacaataca aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg
     2221 tatgagatga aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg
     2281 aatcaagcga ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg
     2341 atcgtaaatg aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc
     2401 ttgaagtcat caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg
     2461 agatgagtta gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa
     2521 aaatacagat gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg
     2581 atcatattgc taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga
     2641 tacatcacaa tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat
     2701 atgatctatt agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg
     2761 cagagttaaa aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga
     2821 taatgcaacg aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac
     2881 aattaataga agaagattta gagaccacag ttcagagatt agcggaacga atccattttg
     2941 caaattatca aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa
     3001 tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga
     3061 agaccatgat ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag
     3121 aggcaaaaga aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga
     3181 aatgcgttga tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa
     3241 tagattttat tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag
     3301 atcatgaaca gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata
     3361 acaaggtgtt tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac
     3421 aaagaatgta tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta
     3481 tatttaaaac agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa
     3541 ctgcatgtgg aataatagat taaaaacagg attgttatta atagttattt cctgcgctat
     3601 gatgattggt atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaacgt
     3661 aatagaaaaa tgctattacg gtcaacatta ttggagtgag gaagtcagag aaaatataga
     3721 tcgtgaatat gttcaaagaa ttgttagatt accagtaagt gaactaaaaa aatgatgaga
     3781 ttatcagcat atcttttaat gtttttgaat gtggtcacgt taatctttgt agtaatcggt
     3841 gtgactttcg atttaatgga atagtgggaa atctataatg gaaaaaagag acacgaatga
     3901 acatattttt tgaatcaata ttgttgatgg tttttgcgat cagcgcttac cagaaatcta
     3961 aaaaagagta atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg
     4021 agcttgtatt ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc
     4081 atcaaagtag gttttcagta gcgatttaac tgatcgctca tcttgtttga tttggataat
     4141 ttcagcatcc aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt
     4201 atcttccaac tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat
     4261 tccaacgtga gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat
     4321 tggaatatca agtagtaacg aggatgtttc ttttactgac tttttcttta cagcattttt
     4381 tgtttttcca tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc
     4441 acttgtttta tcagtcattt attagctttt cctttctatt aatttatgtt attatattat
     4501 cataataaca taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa
     4561 agattgccac attgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca
     4621 caataaatcc gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact
     4681 gtactactga aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg
     4741 ctgtaacagg tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat
     4801 atggcttatc aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta
     4861 ttgatcctcg agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca
     4921 aacaatttac tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata
     4981 cagcattagt tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc
     5041 aattggactt tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt
     5101 taaatgcttc tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt
     5161 cagctgattc cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga
     5221 agtacatgaa aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat
     5281 ccggaggttc aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag
     5341 tagaagatgc ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag
     5401 aaggaaaaat taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt
     5461 tgaaaaaata tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt
     5521 caccttttaa cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc
     5581 aattatacga tgggaatcaa ccgactactg gaaatggtgg ttatgtatgg gaaagctaca
     5641 aatcggcagg agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcag
     5701 atggatggtt atgggctgtt ggcgatgcag ggcatactgg tgtagttata gcggtgtttg
     5761 aagatgggtc atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg
     5821 gagtttcggt aactttgatt gatggagtgg acggatcaga taatgtgcat ttctttagcg
     5881 gtgtaggaaa agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg
     5941 aatagtaaag tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg
     6001 cttgttggaa tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt
     6061 gtgcatattg aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga
     6121 ttacggaaag atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaataag
     6181 tttgcagatc aatcaggtga agaatttttg agaaaactat ttattgtaac aggtaaacca
     6241 ccaattactg ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt
     6301 aagctaacaa actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat
     6361 tttgaagttc aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat
     6421 aatgataaga aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa
     6481 aaaggcgatc ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa
     6541 gaaaaatgct tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga
     6601 agtagtcaag aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg
     6661 gaatgctgaa atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc
     6721 atgggaaaaa tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa
     6781 gtctagtcct gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag
     6841 ctatactgct gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca
     6901 gacaagaaat actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg
     6961 agaaaatcaa ttgttttact tggaggattt agatagctat gtaagagcgg agtaaaataa
     7021 aaaaagagct aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc
     7081 gaaaaaggat tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct
     7141 cagaattttt attaatcaat tcatttagat tataataaat tgagctgaac attacaatac
     7201 gtatatgttg taaaacgtaa aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa
     7261 aactattaaa gacaatatat atggtttatt tgacataaga aaatggtcta gatgataatt
     7321 gtaatataga attgtaggct taaataaagc ataaaatacc ttttatgata aggtatagct
     7381 cttaatttta agcagttttt agatataaga gtgaaagtaa agtgggggag aataataatg
     7441 gcagatcgta aattagaaaa gttattagaa gaaacgtgga atccaaaaga atttagtgaa
     7501 ttttttatgg agaattttga aactgactta gcagtgattg ttaaagatgc tcttagagag
     7561 caagggtatc ctgaaactgc aaactatata aacattaatt ttactttgta tactgaaaat
     7621 aaaggaactt gggatttttg ggctacttta gcaaataagg agttaagtga taaatcagac
     7681 acaggaattc gtaacttttt tgaatcaaac agagatgatt atatgtatgc taaccatcaa
     7741 gataaattga actttagagt agagtttgat gaaacacctg aagaatttat agaaagacaa
     7801 ccgcctaaag aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt
     7861 tcaacaatta gcgaactagg cggacaatat gaaccgctag tagaagcggt tagagaagag
     7921 ctaagactga ataagtttcc tgatgttcaa aatattgatg tttctcaaat tgaaataaac
     7981 gtcaagatca cgaataaact tgattatggt agttgggcag atatagcatt agaaaaatac
     8041 atctactcaa cgttaaaaga gtttatcgaa aatagaatgg atatcatgta tctacagcat
     8101 ccacagtatt taaactttgg tgttgaaata gctacacctc ttgaagaatg gaaaatggag
     8161 caagggcttg actagccttt gctccatttt tttattttac aaatgaatac agttcgtcgt
     8221 ttttaccaag aatgattgtt tgcccaactt tgagattgtc aggtgatcct tcagcggttt
     8281 gtaaggagaa cgttccgttg aacttatcgg ataccgttat aggtagcttt aattctgatc
     8341 cttcctcatc tttgagaatt gctgtttttc gttcatcaaa ataaacagtc tttattaaat
     8401 aatttttcgt agtgatctca gcagatttgt ctttttttgt agtagtaaca gtttctttag
     8461 aattagaggt attttctacg actattccat ctttggcatt tgagcttgtg tttatttgat
     8521 tgaatatttt cagcgacaag ataccgctaa gaatagtcaa tatagaaaca agtgtaatta
     8581 atactgataa tttccttttt ttgttcattt tttcttaact tcctttttgt tttgattagt
     8641 tagatattcc ttatctaaca agccttttta ggctattaaa tgaagataaa ccccaaaaaa
     8701 atattgcaat tatgacagtg caatggtact attattatac aataaatttt gcgaaagggg
     8761 taggtctaaa tcgcaaacaa aaagaaattg aacctagaca aatacaactt gtacaccgca
     8821 agggaagcaa gtttgatttt gggtaaaggt agaaattatg ttacaaacat aatcaactat
     8881 tatccaaatc gtttgcctaa aggttcgttc aagaaagtgg gtgacaaaac ctatattata
     8941 acggaagaag gaatagaagc attgcgatca caaacagaaa ataaaaacaa aaagaaggaa
     9001 tgaatatgaa gaaaaattcg cttggtatgg ttttatttgc atcagctgta cttgcggggt
     9061 tatcagtacc agtaacctca catgctgatg aatggactgc aaacacaccg gatcaaatcc
     9121 aaattacaac aggacaatca agttatgcgt tcaaaaaagg agatactatt tggtcaactg
     9181 tagagaagct taaaagctta ggttacaaga ctactgttgc tgagtttgcc gaattgaaca
     9241 atgttgattt ggcaaaagga gaacaacgga ctatcccaat tggacgaact ttcaacatca
     9301 gtaaagatac attaacagtt aaggaagcag atggcgaagt agttagtcaa actgttcaaa
     9361 aagataaacc tgatcctgtt gaaaatacgg agcaagagaa caaaaaatcc gagactgcaa
     9421 aagaatcgga accaacagca aacacttctg ataagacaga aacaagtgct tcaagtgttg
     9481 atggagcaag ttcatctaat acaaataatt ctacaagtga gaaagaagca tcacagggaa
     9541 caagtgaaag tagcactacg gaaactgata ctggaacatc aacaaataac tcacaatcaa
     9601 catcaaatga tgcaaatgaa tcaaccagta catcttctag tgatcaatca caaacaggtt
     9661 cgagtgatga aactgacagt tctacttcac caagtaaacc cgaacaacca actaatgttg
     9721 tgctaggcgt tccatttatt agtcaaggta acacaatgct ttgcgaagga acatctttgc
     9781 tagaagcgtt acattataaa ggtgttgcaa caaatcaaga tttaatgact tttgttaaat
     9841 caatgccact atctcctgat aataatccaa ataatggttt ttctggtgaa tggagacaca
     9901 atgtggatgg aacttatcaa ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta
     9961 atggcggaaa tgttgtagat atttctggtt catcggtaca aggattaaaa gatgagattg
    10021 taaatggaaa tcctgtagta gcgtgggtaa cttatgcctt tgaacctgct caacataaat
    10081 caatgttttg gggcgatgct atttggaatc gacatgtagt agccgttgat ggatttaaag
    10141 acggttttta ccatgttgtc gatcctgtgt ttggggcaag ttggattaat gcaggatcat
    10201 ttgaaacagc atataacgta tctcaattag cggtttcggt acgttaataa aagagattgt
    10261 ttatatacta agtagttaag tatagtcttt atttgtcctt gcttaactac ttagaaaact
    10321 tgttgctcat ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac
    10381 agcttgcttt caacgaaaaa agtgattaat aggtgtggtg attttaactt aggaatgggc
    10441 gcgaaggtca ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa
    10501 gaaggagtaa cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta
    10561 ttgttttgcg actttagtaa tggtgttatc tacgcttata atgccttttt cagcaataag
    10621 agctgaagca gatgtcacaa aaccgacaag tgcaaaattt acagaaactg gcgtaaaaac
    10681 agacggttat attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca
    10741 atttaaagat tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac
    10801 aggaagtgga gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat
    10861 gtttaaaggt ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga
    10921 agcatggtat gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca
    10981 gattgtatgg tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt
    11041 tgaaaaaatt tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat
    11101 cacagcttct aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc
    11161 aaataagact ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat
    11221 ttatgatgca gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac
    11281 tattaaagta cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt
    11341 tagcacaacc aatcctttta cttttgatgg acgaggcgtg tatcaagatg ccgtagttat
    11401 gatcactact tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt
    11461 tggtcaggta acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt
    11521 cactctaact gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc
    11581 tacgtttgat ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg
    11641 ttatcaaggt tcatttaaaa aggatggtat tactgttaat accgatggac aaaaatttag
    11701 ttacactgct acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga
    11761 tggaaaacca ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc
    11821 agaaaaaaca gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag
    11881 ccttgaagag accggagtac caactggtta tacaggcgca ttcaagcaag aaggaattac
    11941 cgttaaagat gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggtgt
    12001 agtaaaagtt cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt
    12061 aaccgatgaa aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt
    12121 caatattgta gcaaacaaga cgtatagtct agaagaaacc ggagtaccag taggttacac
    12181 aggtgcattt aagcaagagg gtatcagtgt taaagacgat gggcaaactt ttgaatatac
    12241 agctacaaat aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa
    12301 acctttagca gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa
    12361 gacggatgca actggtctgg ctacgttcaa cattattgcg aacaaaactt acaacttaga
    12421 agaaacaaaa gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa
    12481 agatgatgga caagtctttg aatatacagc taaaaacact ttgaaaaaag gtgtgatcaa
    12541 agttcataaa gtcgatgatc aagggaaagc attagcaaac gcagaattca ctttgacgga
    12601 cgacaaaggt gtaaaagttg tacaaaaaac ggacagaaat ggcttggcaa catttaatat
    12661 tgttgccaac aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa
    12721 atggtctaaa gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat
    12781 taatcacctt gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct
    12841 tgtatttcta attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc
    12901 aaaataaaaa agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa
    12961 atcaaaaaaa attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg
    13021 cgcaagctca atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa
    13081 ctataattat caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga
    13141 tgggaaccct gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt
    13201 aaaacctctt gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc
    13261 cttgggatgg acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg
    13321 attattacta aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa
    13381 tatagacata cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt
    13441 tgaaacagga acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc
    13501 tgaactaaac ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa
    13561 ctatttccaa ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc
    13621 cggaactgtt tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa
    13681 aacagacagc acaggtaaat taactgtgga tggtttggaa ttaggtgact attatttcct
    13741 agaaaccgat ttgggaacag atgctaatag aacaacctat ttactagatg gtaaaaaatt
    13801 caaatttagc gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa
    13861 acgacctgta attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca
    13921 atatacattg aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa
    13981 agatgttcta ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt
    14041 agatactgat tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc
    14101 aggtatggat aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat
    14161 gaacgctcat actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac
    14221 gatctacaac aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat
    14281 cattggtgat caccaattca ttaaaacaga cggagataca agcaaagcat tgagtggagc
    14341 aaaatttgta gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc
    14401 aacaacgaaa gctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg
    14461 atcagacggt aagttccaaa ttgaaggttt gcaatatacc tatgatgtag attttgataa
    14521 acaaggtaat ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt
    14581 tgctccttca ggatatacac caactagaaa cgatgttcca tttgtggtat cgaatgatga
    14641 tgcttctagc gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg
    14701 tggaattgga acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt
    14761 tgtctacaaa cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg
    14821 ctctagggtt tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat
    14881 atcaagttga tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct
    14941 gtaattactc aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa
    15001 aaatatgatg aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt
    15061 aataggaaac gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat
    15121 attccgtcaa atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt
    15181 ataccaaaga ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca
    15241 gcaggagcag gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct
    15301 gttatatcag ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc
    15361 aaacttggtg ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat
    15421 cggataaaaa caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat
    15481 tatgtaacac ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga
    15541 ggggttagca taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta
    15601 aaggtaaagg cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata
    15661 gctgtgatag tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa
    15721 taaaatacta ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa
    15781 tcagctctat catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga
    15841 gaaaaaacaa actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa
    15901 cagcggtaac caaggattag acaatactca gaaaacagat aaaggagtag acaatactca
    15961 ggaaacagac ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac
    16021 aggagatgtt ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc
    16081 ggttggttat gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc
    16141 acaggagcat ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg
    16201 taaaggtcat caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct
    16261 ttatgttgat caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc
    16321 agtttatacg gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac
    16381 tgatattcct gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta
    16441 cgatcgttta ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt
    16501 aacagttggc aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc
    16561 agaaactgtt acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt
    16621 agatggaaat aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac
    16681 gtttgctgaa aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct
    16741 tttagtcatt tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat
    16801 caaagagttt agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg
    16861 attgatacta ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa
    16921 aataaaaact gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt
    16981 atcagaatat ttatcagaat attattagaa aagataaact caaaagctta atttaatagg
    17041 gttattggtt gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta
    17101 gaaaatctta tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta
    17161 atggtggaaa tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt
    17221 gatggtgcag aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa
    17281 acactgaaaa aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat
    17341 gctacatggg atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt
    17401 ttagataaaa ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt
    17461 aaaacgttct atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg
    17521 ccaaaacaaa cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca
    17581 gatgacagag aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa
    17641 atggtgcaag aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac
    17701 gacgaagatt tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca
    17761 aatttaggag aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta
    17821 cgatcatatt cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg
    17881 atgagctatt taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa
    17941 ctattaaaag aagagctata gggattattt caacataaac acaagcagaa aactaccaaa
    18001 caaaacgtac catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat
    18061 caaaccatga gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga
    18121 aatgtcgaaa atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca
    18181 gctgtaggag aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa
    18241 tttcctttta tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta
    18301 gttagttttt ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa
    18361 atgaataatc aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct
    18421 tttgagtttg aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa
    18481 agtatagcgg atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa
    18541 acagaaagag atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt
    18601 gatcccaata tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat
    18661 tttgaggaag ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag
    18721 gaagtggacg aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat
    18781 tataaatatt tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa
    18841 aatgatattg aataaaatag agctgaaaag ctctattttt tttgataaaa aaactagcta
    18901 tttttttaaa aataatttgg ttcaaaattt atttttttaa cttgataatt ttttgccatc
    18961 cttttttaaa ttggctatat atcaaggttt atagtgggga taaataaaaa tacatttgac
    19021 aaaatttaca atatagttta ttatatattt atcttattat gataacacaa taaaaggaat
    19081 gatggtatga aaaagaagaa gtttagtcga tcgttgttag gaattttagt tttagttgcg
    19141 gttggtctta gtggcaaggg ttactttcag aataacagca ctcaggaaca tgattttcag
    19201 atcaaaagta gtgttcaggt aggtaataaa gcagacagtg aatcaaaaga atttttaggg
    19261 atacgttatg atccaaacaa atatccaaat aatatcgtaa acattaatga tgggaaatat
    19321 acactgacaa aagaagacca tgaagcatta tcagaaaaag gcagtcaaac cttttgggta
    19381 aattatgcag atttagatca acttggaaga gcaggagatg ttgaagcgtt gatcacctat
    19441 gatgctataa attctcactc atctaaaaat gtggaacgtc catctttcaa aagttctgtc
    19501 catattgcag gagaatacaa ggatggagta tttgatcaag agagagatac ttgggtcggc
    19561 aaagattcaa acaatgaaat cactcagcta gaaggatata aaggttatct ctataacaaa
    19621 agtcattcat tagcatggtc gctaggagga gatatggagg tacacaattt aacacttgga
    19681 acgagagcgc aaaatgttgg gaaaaatgat cagaatggag ggatggctta ctcagaaacg
    19741 attgtacgtg atgcaatcta taaaaataag aatgaaaaag tattgtataa agttgatcca
    19801 atttataaag ggaatgaatt ggttcctcgt ggttctcatg tcgaagcttt ctcagttaac
    19861 gataatggaa aaacggtaaa ccttaatgtt tatgtattta acaatcaaaa aggaatcaac
    19921 atcaactatc aaaatggtac ttgggtaaga gattagaaat tgagaaggtg aagaattgga
    19981 aacaataagc atcaatggat tagataaatc agttgcaaat cgaataaaaa aaggaaatgg
    20041 ctactatctt ctgaagaagg gtaaatattc agttgctaga gagttagaaa caatgagtat
    20101 cgtgtatgta gctaagaatc attttgacgt agaacaggct atttttgatc cagattctga
    20161 aatagggaaa tgtgttgaat tatcagagaa aactgttagt acaaaagggg cgatgatcat
    20221 tgagagagaa atgttagacg aaatcagagt aatcattcct aaagatacgc agatgtgtga
    20281 ggacagcata agtgacgatg aaccaaaatc agaaagaaaa taaggagata ttcagatgag
    20341 cgaaaaatcc attgaattaa aggaatataa gcgaagcgat ggagaaaaat attatcctgt
    20401 agccagtttt tctaaatcag tatatctggt tggacgtgca ttggtagttg gtgcaattgg
    20461 catatatatt ttgcattggg taacaaaggt tgtacaactg cttgtagata ccttagtaag
    20521 tattcttgtc tcgttaaatg atgttaacgg aacagatgat ttaggattaa aagaaatgtt
    20581 taaagagtta ctcaatgcgt tttttcccaa ttatttaagt tattcatata atgtacaaag
    20641 cttttttgca aagctacact tggtagacat ctctatttta gtattagaag caggaacggt
    20701 attctttgtt ggatatttgg ttactttgat gttcaggcat cactatggag aacaaagacc
    20761 attttttgat gatatggaaa gtagaaggtt gaaaagagaa gctctagaag ctatggatgt
    20821 gaagtatgat ccaaacaaga ttataggaga tgaaacgaac aacaaaaaaa gaaaacgaaa
    20881 caaaacgcaa ttagaatccg atgtgctatc aacgcttaaa aatatcaaaa tttctgttca
    20941 tacaagacgt gatctaagtg tcaatgaaga tattcgagaa tatatcgtaa aaatcccgca
    21001 accaaaaggg aagaaagtta gagaaacagt tttacgacaa attcaagatt tagggtcgat
    21061 cctgacaaga gcaacaagcg gacgagccaa atttggtgaa aaagaagatg aagtttcagg
    21121 cgattatttc attttccgag catcagtaaa tattgaaaga gaacgtaagg aaagagtaag
    21181 taccaaaaac aaagaaacgg aaagagcaac atcttcaatc gcagtttcaa attcacaatt
    21241 ttcttttcca atcaatctct ttgccgataa ttccaaaaat atccaaaacc aaaccgaaaa
    21301 agctagaaaa tatgccgaaa ctcaaaagca atctttagaa ctttatctat cttctgctga
    21361 cgtacctgca agtctaacag atatgcacgt tgggaataca tctattgaat atttgtttac
    21421 tttatctcgt accagaaaaa ttcaactaag ctcaatgaaa gaagaaattg aaacttactt
    21481 gcatatatca ggtgtaatcg tgcggatcaa cgcagggcgt attcaaatta ctctaccatt
    21541 aggagaaaat gatcgtattc caattgatgt accatcaatg atcaatagcg tgttttaaaa
    21601 gacatcacaa atagaaacag aaaatacaaa ctaccagttc acaaacagaa aagtaccgag
    21661 aaaagtgagg aatagaaatg gctatgaaat tattagagaa agtcaaaaag agagccgaaa
    21721 aagctgaatc tattttgcta gataaagaac ctaatataaa gcttaatgga aaattcagtg
    21781 tcaatatgtt gaacacaaag gctaaagacc cgacagatgc tatttttgga attaagcctg
    21841 atggcacaat tgctcatttt tgcatcagcg aaatgccaca catgttggtt gcaggaacga
    21901 caggatcagg gaaatcagta ttactgaatg aaattcttgt tacagcaatg tgtcactcaa
    21961 cgcctgatga attaaaaata ggaattgtag acccaaaaag ggttgaattt ggaagataca
    22021 aaaaattgcc gttcatgttg gctgatccaa ttacagatat ggatgaagct tatgattttt
    22081 ttgaatatct tgtgatcttg atgcatgaac gttacaaact gatggaaaaa gcagggttac
    22141 aaaacatcac gctatataac gaatatgccg aaaaaaatgg attagataga tttccctatg
    22201 tgatcttatt agttgatgaa tattcacagt tggttggaac acataaagaa gtagaaggtt
    22261 tgattgttca attaggacaa atggcaaggg cagcaggtat tcatgttatc ttagctacac
    22321 aatctcctcg atcaacagtt gtcactggta ttatcaaagc caattttccg tcacgtgttt
    22381 gccttatggt agcaagtgat ttagaaagta ggatcgtatt agatgaaggc ggtggagaaa
    22441 gcctaagtcc aaagggagat atgattatta agctcgttaa tggaagtatg gttagagcgc
    22501 agggagctta catcagtaac aaagaaattg aaacaatttt caatcacctt agaaatacta
    22561 tgccagaacc agaatatgtt gattaccgag caatcgtagc tgaatctcgt ggtgagggtg
    22621 gagaagacga ggattttggt ggaattggta aagctcctgt aagaaaatct gctaaaagaa
    22681 atttgaacac acctttagca aattcatcat ctaaagagcc atcaattgaa aagaagagta
    22741 cgtttgctct aaaacttgaa aaaaacaaag aagaaaaaga aaaacaagga gctaaaacac
    22801 tagaaaaact tcaagccgaa aaattagaaa aagttttaaa agcattagat aaagctgatt
    22861 ctgaaccgca aacctcaaac aacacgaata atgaacagcc gatttcatct aaaactaaac
    22921 cgaaaactac tgatccgaaa gtttcagtaa acgtgaccat cacaaccgat aagtctgaga
    22981 agaaacaagt aaacggaacg acttcaccta tcatgcaaaa aatgttgaaa gccaaaaaac
    23041 aggtgcataa atgatcattg tcagttcttt tttgaggtgg cttacgccat ataaatatac
    23101 agatgaagta gttgtgaaac gaacgaagaa aagagataaa aaagcggtac taactcctaa
    23161 acagatgctt gatcatggca ttttaggggt gaaggtttta gaagtgaata aaccgtttga
    23221 atcaaatagt gcaagagaaa agttgcttgg agctgaagcc gatgtcgcat atttttacca
    23281 tgacaaaagc aagcatcaaa tagctattaa attcaatcca aaatcggcat ggcggtatca
    23341 agcaagagat gacttgaaac gtaggaaact tagaccaatg ctctatccag aaggaaaaga
    23401 agcatttgat agaacccaca tcgttcccat tggttttcat ggtacagaat cagataaccg
    23461 cctattagta ggttggaaca gcaatcaaaa tagagttcag atcaataaat ttgagaagaa
    23521 agtaaggaaa ttcaacgatc gaaaaacgat cctatggttt gcagatattt gtaaacagcc
    23581 ggatggaaca gcaaagtggg aagcggtgat atttgacgaa aatggtaaaa aagtaatgtc
    23641 tgaaacatgg catgataaag ctaaatttgt ttggcagtag cagtagaggt gaaaaagatt
    23701 gctagaactt tttgaagcaa atagaacagg ctttaatcaa aagttagaag tgggagaaac
    23761 tatagaatat aaagataaaa actatattat tatcggaatc tataacacga gaattaacag
    23821 aataggtgat ccacgtataa ccagtgactt ggtttgtcaa tctgttaact attcacctga
    23881 tctgacaagc aggtataaga aatacgtgtt attagaatat agacacaaga ttactgatga
    23941 aattggtgta aacaacacac gaaacatatt taagatcgga acagtgattc cttatagtaa
    24001 caacgaagaa aaaatatttt atcagattta cggaatagaa aagtttgaat atgaacatgt
    24061 cgatctgcta gtgacgtacc aaatgcgttt aatagaacct tggtcacagg cagaaattga
    24121 taaagcagta aaattgaacc gattatcaaa atttaacgta ttaggaaatg ccttttaaaa
    24181 atcgcaatac acaaacagaa aaataccaaa aaattagcaa tgaacgtagg taagaacatg
    24241 acaataaata ataataaccc taaaaaatat aatgctcttg tccaatgcta tatcgaaaac
    24301 aaaattacaa tgcaagaagt cgctgatgaa ttaggcaaat caaagagcct agtatcacac
    24361 gattttaagc acaatgtttt tccgaacccg attgagctga ggaaaaaatt tcaaaagaga
    24421 gtttatcaag aaataggtaa gtggattgat gaagggaatg caattgaccc tttaattgaa
    24481 aaatatcctt ttttaaaaag caaaaataaa attcaaaggt taattcgtca aggcgatatt
    24541 caagctaaag aacctgtttt gacaaccaat gatttggcta aaataaatct tcataaacaa
    24601 attattgaag actttgaaaa aaatctttca acaactgaaa ttttaaaaaa atacaatatt
    24661 ccgagaggaa agctaacatc gactcttttt agagaatatg gagaatcggc agttactaaa
    24721 gcaaaggaag tagcgaatga gagaaaaaaa caaaaatagt agtcgatacc tagtagcgac
    24781 tgtagttttt gtttgtgtat tgattttcct gtttgatttt ttgcagattg gaaaaagcat
    24841 aattggaaat acattgagtg caaaggaaat ggttgcttta ggtggtttgt atgaaggaca
    24901 cttgttcccg aatatattca tttctatcgt ttctcatatt aacttgtacc atttaatttc
    24961 taatttattg ttcttagtag ttcttgaaga agcactgaat gatcagtatg agtggttaac
    25021 attattgatc gttttaatca tatcaggatt tctaggaagc ttagttcctt ttctgctaca
    25081 cagcaacatc gtaagtatgg ggcttagtgg aggtattttt ggatggattg ctttatttgc
    25141 gattgcatac atccgttttg atagcaagaa acatttatta ctaagtgttt ttgtattatt
    25201 gataatcggc attttactgg tatctacttt tgaatcaggc gagacaaacg tcttgtcaca
    25261 tgtaatcgga tttgtctgtg ggatgatctt gggatttatt ctaaagaacg tttcattgat
    25321 caaacaaaag aattaaaagg gagtgctaaa aatgccaaaa aaaaaagaaa agaaaattcc
    25381 agtgtttttg tctgtctcag aaatgttatc acttcaaata ctgttaagaa aaaagattga
    25441 aacaatagac gaagaagtta gaaataaaag ggctatgtac gaacaacgtg aagcgctaag
    25501 agaacgaatc cactattttg atgatataca cactaaaatc tatgaagcac gtacaaatca
    25561 cgaagacgaa agcaaattag gattagctca acaacgaata tttgaatggc tgaatacgaa
    25621 tactgagcaa gaaccgttaa gagcaattag taatcttttt gaatttggaa cgaagcattg
    25681 gtttaacgct gtagaaaagg atgctgactt acaagaagcg ttcgatacac taagcgagga
    25741 tagcaaatcc atgcgagact tattgatcac ttatctaaat gagcatgaat ttgaacagac
    25801 gaaagttaaa aagaaaaata aaaaatagaa agttgtgatg tatcatgctg tttaccttat
    25861 taggttattt atgtttagtt cttgcttttt ttggagcaat tgctatattt gtcggtcagt
    25921 ttcaagaaaa taatccgata atgcgtaaca taggcattgg aattgttatc atatccttga
    25981 ttgtatttgg tgccacaaag atttttggga gtaatgatca gcagtttggt atttctggag
    26041 ttgataatag ttatgctcca gattcattgg gaagagtagt gatagatgga acgtttgaga
    26101 atgtaacgag ctacaaaaat gtttcattga cagtgaaaga agatgacaag aaagcatcag
    26161 atgacgttct cgctacgatc aagccgtctc aatcacagtt tgaagtgttt tatacagtca
    26221 aagagaataa ccctgtacgc aagctaacat tagtttttaa cgttgacgga gaagaatact
    26281 ataaagatgt aaaaatcaat aaagcatcaa cgaaaaagat tgtcgaatca gcgaaaaaag
    26341 aagtagctac agaagaaagt acaactgaat caagttctac tgtcgaagtt gaactaccag
    26401 ataaaattga ttttgatcct gaaaaagtca aatcgtttga atctgtttct ggttctatgg
    26461 cagatcggtt taacactgaa gttttcaaag ctatcaaaac tgttaaattg gatactgaaa
    26521 agcaaaggat taatgttacc ttattcaatg cagttgaatc tatggatcag gataaaaaag
    26581 ttacggttgc aagagaacta gggaaaagag taattggatt gtatcgaggt acggcaaaac
    26641 cagattcaga cggttcagat ttgccagttc acgtagttta tgaagacggt aaagtttttg
    26701 gagactctac agcaaatgaa gcactaacga ttaaattagc taaatagccg gaaaattata
    26761 ataaacaagt ggtgtcacac caccatcgca taggtgtgac accttggaga tgattactga
    26821 tggtagtaaa taaagaaaaa gtaagaaagt atgttacttt aaaaatagct tctgttgaat
    26881 atttagaaaa gcgagctaaa gaagaagaaa aaacaagagg taagaaggta acagtttcag
    26941 agttgttaga agaattaatt gagagtgacc aaacaataag acgtgcattt accaataggt
    27001 ctgttaaaag ttagggtgat actatcttta atttcaacca aatttctaat atatgatgaa
    27061 tagtttcctt gtacaaaata tctaattaag agataaaagt taggaggatt cattcaaaat
    27121 gattatccaa aagtttgact ggaaaatgaa attaggaatt agtcttgtaa tattgggatt
    27181 tattattttt ttggggataa aagcacaaaa aaatcaatct acttttgaaa ctatgtcttc
    27241 tcaaattatt gaggaatgtt ccaaaattta ttattgtgag aacaaaactg aagaatctaa
    27301 tggcttttat tatatcgatc aaccacatga tgttgttata aaaatggagt cacatgaagt
    27361 aaacgaatgg attcaaagaa gtactaatgt atcaaaaaag ttagaaagat attctcaaac
    27421 gtttttaagt aaagtcattt tttcaaattg caaaaatgaa gaaacgtctc aaagagtagc
    27481 tagttcttta gaaaatttaa aattttctgt ttcaaaaaaa gagaaatttg tagatttacg
    27541 aaaataaact atcgaaagta gtatgattat gatcttaaaa tatcatctta agagaagaat
    27601 tacgattgtt tataggattt acaaaatgaa taagtttgaa gagtgatttg gtcgcagact
    27661 agatcactct ttttgtgtaa aaaaacttta cacttatttt tgagtgtgtt aagatttttt
    27721 tatatagagt gacggtttct tgtcagagtg gatgataacc atcaatattg gaggaataat
    27781 aagtggaaaa caataatgat attagatatt ttagcattgg ggaaagagtt acaacagtaa
    27841 gtgaattatc aaaggattat agagataaag aaggaacgat tatttctctt aaaaagaatc
    27901 gaagaggtgt ttggcaatat gttgttgatt taggggatgg cgttacagtt gagtgtaaag
    27961 atagtcaact gatcagaaat gatggcagtc attccattga aaaagcaatt cttgtattcc
    28021 caaaagtatc aagcaagatt atgaatgact ggttgcttgg aaaatttggt gagtacaata
    28081 aaaaagaaat cgcagaagtg atcggcaaat cttatccaac agtattaggg tatctaaaaa
    28141 agataacgaa caaagaagcg aaagttgagg atgttttaac agtaagagaa cttcagtcat
    28201 taatcgaata tcggaataaa caaataacaa atcctgagta ccttaaaaaa gaagatggtc
    28261 tattgaaaca attggaacag gtaaataaaa tccggaaaat gcaaaaggta gctcctttaa
    28321 gcctagataa ctttaaaaaa atctatttga aagagatcga aaaagactaa tgtggagtgt
    28381 cactttaccg tttcaacctg tatctgcacc aagaccaagt gcaagaagga atgttgctga
    28441 tgcaactaat gacgtagcag agaaaaggat ttcaacctat tatgataaaa agtatctcga
    28501 ttatttgaaa aaaataaaaa gctttatcga agatcaaggt ttgttagatg atgaatttta
    28561 ctcgatcgta aatgatccga tgggtgcaat tatggaagtg gacttttact atcagatacc
    28621 aaaaagctat aaaaaagtct ataacattat gaaaacgaca gcgccagatt tagataattt
    28681 agtaaaaagt gctatggatg gtattttcaa catctcagct atacgagata gccatatcac
    28741 agggttaatt gcttttaaat acaatgtggc aaacgaagga aaaacagttg tgaggatcaa
    28801 gcgatattca gaatttgagt gggatacttc tgaaggattg aacggagata tttgggaagc
    28861 cacatttgat ttcaaaccag tagcaacgcc aagaccaaag tctaaattta gaggaaatgg
    28921 gattatcact tactatccaa aagagtataa cgactatttg gaaaatatca agaatacgct
    28981 taaagagaaa gatttggtaa atgatcaact gaaaaaggta atgaatgctc ctatgggggt
    29041 tatagctcag atagattatt tttaccaagt gagaaaagat aagaaaaagc ttgattcact
    29101 gatgcgtact tcacagccgg atattgataa cttagtcaaa ggaacattgg acggaatttt
    29161 taacaaggaa attggcatca aagatagcag ggtggtaggt ttaatagctt ttaaatacaa
    29221 tacttttgaa aagtcgaaaa caaacgtgcg actacaggag atgggctgat gaataataac
    29281 gaaaaaattc ttcatgtact tgatagcttt gaaacaattc aagaggaact aaagaaatat
    29341 cgagatgttt tggaacaaag atatgatttt gtcaatcagc aaaagagtaa tcacatggat
    29401 ttcattctca atatgaatga cttgaagaag aaattagttg aacgaaaaga acaagaaaag
    29461 ttgatcaaag cttactttga actcggtgaa aaagaagtta aaaaggcaat ggaactgaat
    29521 gaagatcgca gggttttaga tcagctgtta gaacaattat tggtgatgtt tcaaaaagga
    29581 cgaatagatg aggatttaat tgaagaaggg ttgagaaagt atcctgcaaa ctcaggtatc
    29641 ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag atttcatacc ggcagaggat
    29701 tttgaatcgg caatggaata tataaaatat tacagccaag ggatcactgc ttttagagag
    29761 tttgatcctg aagatgttat tcatgacttg aacaacctaa aagaatggtg tgaaggttat
    29821 gaagttgatg attccggttt ggactacttg atcagtatta tggaaatcga agaagagatg
    29881 ccggataagc ctgatccaac agatatttta gaattaatcc atgatgctag aaatccaatt
    29941 gcatacattt ctagaggata tactgtattg gaatactata agccttatat atcagcgatg
    30001 aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc gctcagtgtt aaatgcatcg
    30061 aatagattag aaaaagctgt ttcagagcta gatgcttatt atcgtgaaca ttacctgcaa
    30121 gcaggtggta tgccaagaaa tacgaaagca aatatcagta ggtatataaa agaagctgag
    30181 taggcggagt tagcccataa aatggcaaaa ggtaacctga aagaggcgtt ttgaatgaaa
    30241 agggatgttt ttgaattgta cgtattagaa cgtctagaag acggcatgat ggcacaagag
    30301 ataattgatg aaattgatga tatgatcagc agatttggtc aattggaatt gaatgatgga
    30361 aacaaaaatt cggtagtgct acaacaattt tggttaaata atttttcgta taaggatttt
    30421 atcaaagaaa aggcaaatag attgtactga ctaaattcac gaggatcagt gatgagagct
    30481 tattgctaag agcagttagg ctcttttttg tttgtgttaa attattttat tttacaatac
    30541 acatttatac acaaaaaatg ttgacggata gtacacaaat gtggtaggat atatttgtaa
    30601 gcaagttaga ttaacaagtt aaaggaagga agagcagtta tgaaaaaaga agaactaata
    30661 gatatgtttc aaattgttga acgagcaaat aatatgggaa ttatgttttt tgatcgtatt
    30721 tctttaaaga tggatttaag cgttgcccac caagaattta atctacgatt aaaagcttta
    30781 ttgatttctg atgatgttaa tttcgctcat gatgttgtag gtatacagaa ccatatagat
    30841 agagaaaaca aacgtatggg tgatggattc ttaccaagat attcaagttt atgatttggc
    30901 gaaagattca agagtatgtg tcattattga taggtactct tgaattggaa aggaggaatg
    30961 aatttaagac aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac
    31021 gaataatttt catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa
    31081 atttttagta ggaattctat tcacaatggg gctagtgcta ttatcacctt cagtttcagc
    31141 agaaagctct atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac
    31201 tagaagcgtt ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc
    31261 gtggcaagct ccaacaagtg gttccccagt ttatcgcttg tacaacagat ataacggtga
    31321 acatttttac accgttgatg ccggagaaag agataagatt tcgcacacag gatggacgta
    31381 cgaaggtgtt gcgttctatt cctacaacgg agcaaataga gttcctgtga cacgcttgta
    31441 caacaaatac gtgaattggc accattacac gttagatcaa aatgaaaaga acgtaattag
    31501 taaacaaggt tggaagattg aaggtacggg ttggtatgca atgccaaaca gcgcaccaaa
    31561 tcctacacct gcacctgctc caagtaatga aaaagtgtta aatgttcctg tcatttggca
    31621 aggtaatacg atgttgtgtg aaggtgcttc cctgttagag ggattgcact acaaaggagt
    31681 agcgacaaat caaaacttaa ccagttttgt aaactatatg ccaagagcta atgacaataa
    31741 tccatatcat ggctattctg gcgaatggcg ctacaacgta aatggtacgt atcaaggcat
    31801 gatgcctgat ccagtagtta aatgggcaaa aaatgttggt ggtaatgccg ttaatatcac
    31861 tggttcagga acgaatggga tcaagaatga aattcgcaat ggtaatccgg ttgtaacttg
    31921 gattacttat aactatgctt cacctcaatt caaacaaatg ccttggggac gtgcagtctg
    31981 gaatggacat gtggttctcg tggatggctt caaaaatggt gcataccatg ttgttgaccc
    32041 tgtatttggc gcaaaatgga tcaatgcaag cacgtatgag agagcattta atgtgactgg
    32101 aatggcggtt gctgtacgtt aattgataga attaataaat aaatgaaata agagcttaat
    32161 tactgaaatg gtaaatgggc tcttattttt tagaaaaaaa ctgttttttg taatacgaat
    32221 ttataacaaa aaacgttgaa tgtagtacac aaaagtggta tattaaaacc gtagagatta
    32281 tgcgaagagg tgattttgat gaaccaaaga agaagaaaaa ggctagaaga aataagcgag
    32341 cgactatccg agttaaagga tgagttagaa gtaattcaag aagaagagga agagtacaaa
    32401 gaaaatatac ctgaaaatct acagggatca gaaagatatg aaaaagcaga agaatccgtt
    32461 gatcaacttg aagaagctgt tgatggttta tccgacatat tagaattgat tgaagatata
    32521 gctagctaga gatgattaat gaagatttca ggagtgagca aaaaatgatt gatatttcac
    32581 tgatagagaa gctacttttt tcaaataagt acacagccaa agagttggct acaagatggc
    32641 aaatatccga atcagctatt aaagcctatc gtcaaaataa gggaagcgta ggatggcgtg
    32701 attggaaagg aatcagcatt ggaaaagcag aagacataat gaaacgtgaa ggatttcaag
    32761 ctgacaatga ttctaaaaaa gttgaatagt ttaatagtcg ccaagaaaag taccaggacg
    32821 aggggaaagg tagattacaa aggaaaacac ttttaaataa acttcgcaaa agcatcaggt
    32881 tatgagtggc tagggctata cgaatgtgat gaaaacaaag ggctttaatg tcaaagaaaa
    32941 cgcaataggg ggggaacaaa atttttaaaa aagatattta tgtttggttt aatggaaaaa
    33001 aatatatgac tactaacaaa aggtggtcag agctagtaca aaatacagtt aaaaaagtca
    33061 tgccagatgg atatcatttc aactatatac aatgctcaca aacaactgaa agtatttatt
    33121 tttcaatatt aaatgagaat agactctatt atttcagggt aagttcacac cataggaaaa
    33181 agagagaaga taatgttacg acatttttat cgggaaaata caatggcttc aaagagttaa
    33241 gtgaagccat tggtgagtat ctaaaaagta atagatttgt cagaatttct gacaaacata
    33301 tattgatctt gttactttat ttgaataata gcttcaaatt gttttggaga gacgacactt
    33361 tctactatat tgagaacggc atggatattt attctggtat caacggcata ttaaaacatt
    33421 atattaaggc gttacttcga tatggggttc tttatcaaga agatttaagt aaccaaatta
    33481 agattagtaa ttctggaaag tcattactgg caaaatataa aaaattgctt tctcctatct
    33541 acaaaggaat agatatggaa gctgtaaatt ctgaaacttt atttaattta gttgttgatg
    33601 aaaagtttgc gaatgaattt tttaaaagta aaaaaattga aagactaaat aacctgtttc
    33661 ctgaaaatgc aataaaagct tatgtagata ataataaatc acgattatac aactggttta
    33721 ttcccaaacg gttgcgagga aaaatcaaaa ttggcgatca agtgaaagta attaatctaa
    33781 atcatactaa gatcgttacc gtaacggagt tgtatatagc tgaaaaagaa gatattacaa
    33841 aaatgaaacc agttattaaa atactagata acaaagagtg agtggaatat atggtagcaa
    33901 aaaagaatac tctaaaagaa gcttctgaaa aatatcaaga tatgaaaaga cgaggattaa
    33961 taccaaagga caaaaaaggt atatggagag aatttgttaa ggaagtcagt agtgatcgaa
    34021 gtggcggtga ctttgttgga cgatatacag caaaaggtta tagcaggcgc ttaaggatca
    34081 ctgttgaata tggaacgaat gaatcagcta cagagctgaa gcaaaggtac ttagatacac
    34141 gaagagaagc ttcaaaaaaa tatgatgatt tcaagagcag aggtctaatt cctaagaaga
    34201 aaaaggggat ttggcgtgag tttttcaaag aagaagatag caatcggaat aagggagact
    34261 tcattggacg atacacagca aaaggctaca ctaagaattt agggatcaag gttgaatacg
    34321 gaactggtga aagttcatgg gaattgagaa atcgctattt aaatacattg ttagagaaat
    34381 ataaagcaga attattggag ttgttcgatg ttaatgattt tatcgagatg gctgaaaagt
    34441 atggtcatga agaaggtttt atgatcaatg acattatggt tattgataat tttacggatt
    34501 tattagcact tgcgaaaaaa gcaagggatt taagagaaac gcagatgtat ctattcagaa
    34561 ataaaaacgg aagaaaccgg agaactgaat cgagtgagat tgaagattac tatgatgcct
    34621 ttacacacga tctgggagcc tttgtagacg agataagtgg tgtaatgaac aaatataggg
    34681 tctagaataa aaaagccgga gatgagcaga taaatgggaa acaatagcat atacacacct
    34741 gaaatgcaga ataaaattaa aagacttgtc ggattagggt ggatgacaag aggaaaatta
    34801 gattctttta gtacgatcaa atatcttttg atggacattt accgtcagaa caagcaaaca
    34861 ggaatacatt ttttgggcta tgataagccg gaagatgcac ctttctttgt tccaagttca
    34921 gcctatagag ccagtacctc cggtagcatg tataacttgg atttgtttga ggaaggagca
    34981 aagatgatcg aatatgattt caacgaagcc tattcgagga tcatgagaaa ttatcctttg
    35041 ccgtcaaaca cttatttgaa agcaaaatat aatacagatc aagctatttt gagagcgata
    35101 aatagaccaa aagtaagacc gtatgaaaat ttaaaaacgt atatgttctt tttggttaat
    35161 tttgaaggaa cagccaaaga aggaacatat gctaatccaa aatcacagtc gatgtttgcg
    35221 gattatggcg aaaatcccaa aacagctaat gagcctattt ggttatcgga gattgagcta
    35281 acacttatcc ttgattatta tgatattaaa gagatggaag tcctagatag ccacacattt
    35341 ttgacgaaaa aaggattact caaagaatat tttgaaagaa tcgagccatt aaaaactgat
    35401 cctgatacgt tgaagcttta taaaaatctt agaacgaaag tatttggtgc tatcggacaa
    35461 aaatcattaa gagaaggaga aacaagcacc ttcgataaat acagtatgta taatcgagcg
    35521 tattcctcca tggtagcagg cgtatttaga gatcgtatgg ttagatatga acagaaatac
    35581 gttcatagcg catatgggct attaaaaatt cgtactgatg gattgtattt cagatccgaa
    35641 gtaccagagt ttgaagagct gtataaactt ggagtagtta agaaaaaagt tcatacaata
    35701 tccaaagaag aagtcgaaaa agctttagaa tttaaaaaca aaggatttga aaataagggc
    35761 tcctttggga aatctaaaga tgaagttaag ggtgttgtga tagacgatga tttcgagttt
    35821 gattttgact aaatactctt tggaaaagat ggtaggaggt aaatatgaag aagaaaattg
    35881 tggaaggaaa tgtaggtgtt attattctaa aagattcaac tgactatgct aaatcaatgg
    35941 cactaaaaga cgaaaaagtt gcacgcatgg ataaagattt agttgaattt ctgatggatg
    36001 atacagaaag attaaaaggg gatcagtttt tgagtttaat gaaagacaaa ggcttaaaca
    36061 catatggttt atttgattat aagaaattag ctatagagtg ggtaaaagaa ggagaaatat
    36121 tttttattga agaatgggaa ggtactgaga acttagttct tcatcagtat cttttgaaag
    36181 cttaatctaa gtaaaacagt tctgactagt tatgcttgtc agaatttcaa aggaagtttt
    36241 ccataaccat catcattcaa aaataaagga tttgaaaata agggttcctt tgggaaatct
    36301 aaagatgaag ttaagggtgt tgtgatagac gatgatttca agtttgattt tgactagatt
    36361 tttttgcaat gtttaagagt aaacaaggta agaactaaga tcatgaagat tgagagtaaa
    36421 gaagatagag gacgaatgta tgagaacaca ctatatttat ttagaagata aaattgtaga
    36481 tgaaattccg gcaaaaagtc gagaacaagc tagaaaatta tttattgaaa aatacaaaga
    36541 gatcaatgga gatagtgaag ttccaaaagt ggtcgtaaca gttagaaaaa ggcaaacaaa
    36601 aaagagaagt acgtttcatg tcagccctga tatatataat cttatagagc gacatagaag
    36661 tgaagataat atttctatct ctcaatatat tgctacagtt attgatgaat tgatttcagg
    36721 aaagtatgac tatgcttttg attttgaggg tgaaaaggat tacaaaagtg ttgctgtctt
    36781 tcaatctgat tttgagaagc taaaaaacta tgctggcagg agaaatgtta attttgcaag
    36841 actactagaa gagttctact ccgaagagtt gggagtagga aatgaataca agagagaaac
    36901 ttatgcgaaa ttgtctcata agttatccca agcgcttaaa gaagtgacag aagttaaagc
    36961 ggaactagat gctaaacttg aagaagtgaa aaaatatcaa acagagagga agaacaacat
    37021 gaagaatcaa gtggaatttg acgaacagaa agaatttgaa aaagcaagaa aacgctataa
    37081 caaaattatg aatgaactta gctatgaaca cttaacagtt catgaataat tgagtgaggg
    37141 tacagaaaaa tatacgatcg cagatatggt taatgaagtg gaaactcttt tagcttcttt
    37201 ctttgaaaat ggtcacgtaa acgatgaatg gcgaaaagaa gaacctaaaa gggttaacgc
    37261 ttatattgct cgattaaaaa ggtttattga taaatataag gagaatagtg acaaattttc
    37321 taaagataaa taaggagaga gtgtgattac atttgccaga aatcgaagga atattttcgc
    37381 caaaaatagg taaaaaacag ttagatttca gcagttttcc gagcgtaaat agagattata
    37441 atctaggtga tgaggtacag atactaaata ttgatttcaa agaactagac ttcgctgttg
    37501 ctgtttccaa caaacccaag gcattggaac gagtgaaaaa aatataccaa gagaatgatc
    37561 aaaatatcaa aaatatgatc aaaaagtata gacgagataa ttcaaaagtt gaagacgttt
    37621 tattcaacgg tacggcaaaa catgatttga atgtttatat ggcattagga gtggctttgt
    37681 actgtaattc aattaatcag acagatattt tggttaatat cgtgaggaat agctatcaaa
    37741 aaatattaaa aataagtagc gaaacaaacc cttattatgc aatgaaatat cttggggata
    37801 atttattaaa tgataaaaaa ttgatgcaca acactgaaga gctgttatct aatttggctg
    37861 cgattaactt tctattcaaa acaggttctg aagattattt tatagataac gtactaaaag
    37921 gtgcttacgg aacagcattt tatccgcttt acaatatgaa acagcataaa gccgaacaaa
    37981 aggataacaa acttgaaatt aaaaagatga aagaactatt aggtatagga tttgtaaaac
    38041 ccaattcaaa agtcactatt gcttcgttag caactactaa caatgatggg aaaatagtaa
    38101 atgggtttga cgattcagat atagatatat tgtcaaaatc atatttttct ttgtatggag
    38161 attttgcaga tttgatgggt attgatagtt acttagtcaa taattttgag atcaagggta
    38221 gcgagctgaa tgatattgtg tatttcgttt taatctctct atatgcaatt gatcagaata
    38281 ccgagtaccg acaaattgac tatttgctag ttttttatag tttgttatat cagttcgttt
    38341 taactcggtt gtttaaaaat gctaaggaag aattgatcct gaattctgat tatgaaatag
    38401 caaaacaaat tgcaagtatt agaaaagaaa acaaatctga agtaatcaaa ttaagaaaag
    38461 aattaagtat tgcgagtaaa gaaaacggtc ggttagaatc tttagtcaaa tcggcaaaaa
    38521 aagaagagga acttttgaag caagagatta aagaattaaa aggaatcaga aaagaacttg
    38581 aacagctgag gtttgaaaat aaagtattaa agcaattgca ggagcaagaa catagacaat
    38641 cattaaatgg taaaactgaa gagccttcac tgtctgaaat gatagcttat ctaaaacata
    38701 aaaacattct tttagtggga ggacatgtaa attttcaaaa taaattaact gaactattac
    38761 caaatattaa aatgtttgat gtaagcgaaa ataatggaaa gatacttaaa aatttgaata
    38821 cgtgtgatgc gatttttcta tatacagatt acttgaatca tggtctatat tatcgtgtta
    38881 tgagtgaaat ttctaataaa gataagatat tttatttgga taatcatgtg aatatgaact
    38941 taacaattaa acgtatttac gatcttttat cacttatata aagagagaag tgataaatag
    39001 gacacaaaat ttggaaagta ggtaagtcac tatgagagaa ctaactttat cttctaagga
    39061 attagaaaag aaaactgctt tattaaacga aatatttagt gctgattgta taatccaaag
    39121 tttaaaagaa agttcatttt caaaactaac agatcaggaa aaaatttctt atatgctaag
    39181 tttagataat ctaggaacta aatatgatga atcagcaagt agcgataagc ttttgattga
    39241 attagtatca agtcttagta gttttggaaa aatgaaaaga tttaatgtga tattagaaat
    39301 ttttgcttgg ttaatccctt catttatttg gtttttttat attttcatag ttgaatctga
    39361 gttaatagcc aaggtctctc ttggactgtt aattccgata taaataggtt cagcaattta
    39421 ctttaataac ttgtatacac gtattcaaat aaaaaagcag ttattacatg aaggattaag
    39481 tggtaatatg ttaaaaagaa ttgcatggga taaagataat aaaacagata ttgagcttaa
    39541 agaatataaa tacaattatt tctcgagatg tatagataga aagttactgt ttaaaaaaga
    39601 aggattagaa tttagttttg atgaagatga atttgaaaaa ttagtgatgg actggcgtaa
    39661 gaatttactg gatacggtac aattctaaaa gcaattgagg tcgaaattcc gatacttgaa
    39721 tcatgatcta tattatcgtg ttatgagtga aatttctaat aa
//
LOCUS       DAYYKC010000014        38297 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000014 DAYYKC010000000
VERSION     DAYYKC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.014"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHBBW_000790"
     CDS             complement(451..1761)
                     /locus_tag="ACHBBW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9152498.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHBBW_000791"
     CDS             complement(1773..3191)
                     /locus_tag="ACHBBW_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9152499.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACHBBW_000792"
     CDS             complement(3431..4999)
                     /locus_tag="ACHBBW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9152500.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACHBBW_000793"
     CDS             complement(5003..5413)
                     /locus_tag="ACHBBW_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9152501.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACHBBW_000794"
     CDS             complement(5410..6333)
                     /locus_tag="ACHBBW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9152502.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACHBBW_000795"
     CDS             complement(6330..7208)
                     /locus_tag="ACHBBW_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9152503.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACHBBW_000796"
     CDS             complement(7195..8532)
                     /locus_tag="ACHBBW_000796"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9152504.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACHBBW_000797"
     CDS             complement(8535..9638)
                     /locus_tag="ACHBBW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9152505.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACHBBW_000798"
     CDS             complement(9712..11220)
                     /locus_tag="ACHBBW_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9152506.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACHBBW_000799"
     CDS             complement(11186..11878)
                     /locus_tag="ACHBBW_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9152507.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACHBBW_000800"
     CDS             complement(11900..12874)
                     /locus_tag="ACHBBW_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152508.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACHBBW_000801"
     CDS             13174..14046
                     /locus_tag="ACHBBW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9152509.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACHBBW_000802"
     CDS             complement(14504..16702)
                     /locus_tag="ACHBBW_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152510.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACHBBW_000803"
     CDS             16934..17926
                     /locus_tag="ACHBBW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9152511.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACHBBW_000804"
     CDS             17986..19407
                     /locus_tag="ACHBBW_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9152512.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACHBBW_000805"
     CDS             19400..20899
                     /locus_tag="ACHBBW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9152513.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHBBW_000806"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHBBW_000806"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9152514.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACHBBW_000807"
     CDS             22491..23174
                     /locus_tag="ACHBBW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9152515.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACHBBW_000808"
     CDS             23176..24012
                     /locus_tag="ACHBBW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9152516.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACHBBW_000809"
     CDS             24023..25777
                     /locus_tag="ACHBBW_000809"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152517.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACHBBW_000810"
     CDS             complement(26053..26394)
                     /locus_tag="ACHBBW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9152518.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACHBBW_000811"
     CDS             complement(26462..28633)
                     /locus_tag="ACHBBW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9152519.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACHBBW_000812"
     CDS             28844..29701
                     /locus_tag="ACHBBW_000812"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9152520.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACHBBW_000813"
     CDS             complement(29772..30629)
                     /locus_tag="ACHBBW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9152521.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACHBBW_000814"
     CDS             complement(30614..33316)
                     /locus_tag="ACHBBW_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9152522.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACHBBW_000815"
     CDS             complement(33329..34618)
                     /locus_tag="ACHBBW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9152523.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACHBBW_000816"
     CDS             34768..35814
                     /locus_tag="ACHBBW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9152524.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACHBBW_000817"
     CDS             35816..37969
                     /locus_tag="ACHBBW_000817"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9152525.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11302 a         7868 c         6810 g        12317 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcagggc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagcatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taaaccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg actcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga cagagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccatt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaattt
//
LOCUS       DAYYKC010000015        34962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000015 DAYYKC010000000
VERSION     DAYYKC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.015"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHBBW_000818"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHBBW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9152526.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHBBW_000819"
     CDS             complement(2020..2922)
                     /locus_tag="ACHBBW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9152527.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHBBW_000820"
     CDS             complement(2919..3611)
                     /locus_tag="ACHBBW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9152528.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHBBW_000821"
     CDS             complement(3654..4295)
                     /locus_tag="ACHBBW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9152529.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHBBW_000822"
     CDS             complement(4333..4647)
                     /locus_tag="ACHBBW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152530.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBBW_000823"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBBW_000823"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9152531.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBBW_000824"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBBW_000824"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9152532.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHBBW_000825"
     CDS             complement(5984..6916)
                     /locus_tag="ACHBBW_000825"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9152533.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHBBW_000826"
     CDS             complement(6913..7689)
                     /locus_tag="ACHBBW_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9152534.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBBW_000827"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBBW_000827"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9152535.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHBBW_000828"
     CDS             complement(10933..12009)
                     /locus_tag="ACHBBW_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9152536.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHBBW_000829"
     CDS             complement(12014..13294)
                     /locus_tag="ACHBBW_000829"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9152537.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHBBW_000830"
     CDS             complement(13313..14239)
                     /locus_tag="ACHBBW_000830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9152538.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHBBW_000831"
     CDS             complement(14252..15535)
                     /locus_tag="ACHBBW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9152539.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBBW_000832"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBBW_000832"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9152540.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHBBW_000833"
     CDS             complement(16323..17234)
                     /locus_tag="ACHBBW_000833"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9152541.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBBW_000834"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBBW_000834"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9152542.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHBBW_000835"
     CDS             complement(17700..18398)
                     /locus_tag="ACHBBW_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9152543.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHBBW_000836"
     CDS             complement(18385..18771)
                     /locus_tag="ACHBBW_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152544.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHBBW_000837"
     CDS             complement(18918..20588)
                     /locus_tag="ACHBBW_000837"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9152545.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHBBW_000838"
     CDS             20771..21004
                     /locus_tag="ACHBBW_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9152546.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHBBW_000839"
     CDS             complement(21103..21534)
                     /locus_tag="ACHBBW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9152547.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHBBW_000840"
     CDS             21613..22026
                     /locus_tag="ACHBBW_000840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9152548.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHBBW_000841"
     CDS             complement(22064..23212)
                     /locus_tag="ACHBBW_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9152549.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHBBW_000842"
     CDS             complement(23334..23930)
                     /locus_tag="ACHBBW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9152550.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBBW_000843"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBBW_000843"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9152551.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHBBW_000844"
     CDS             27160..27591
                     /locus_tag="ACHBBW_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152552.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHBBW_000845"
     CDS             complement(27636..28985)
                     /locus_tag="ACHBBW_000845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9152553.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHBBW_000846"
     CDS             complement(29055..30218)
                     /locus_tag="ACHBBW_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9152554.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHBBW_000847"
     CDS             30347..30670
                     /locus_tag="ACHBBW_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152555.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHBBW_000848"
     CDS             complement(30853..32202)
                     /locus_tag="ACHBBW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9152556.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHBBW_000849"
     CDS             complement(32271..33224)
                     /locus_tag="ACHBBW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9152557.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHBBW_000850"
     CDS             complement(33326..34516)
                     /locus_tag="ACHBBW_000850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9152558.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACHBBW_000851"
     CDS             complement(34716..>34962)
                     /locus_tag="ACHBBW_000851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152559.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAYYKC010000016        34663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000016 DAYYKC010000000
VERSION     DAYYKC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.016"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHBBW_000852"
     CDS             591..1280
                     /locus_tag="ACHBBW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9152560.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHBBW_000853"
     CDS             1267..2475
                     /locus_tag="ACHBBW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9152561.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHBBW_000854"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHBBW_000854"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9152562.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHBBW_000855"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHBBW_000855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9152563.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHBBW_000856"
     CDS             complement(6578..7273)
                     /locus_tag="ACHBBW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9152564.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHBBW_000857"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHBBW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9152565.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHBBW_000858"
     CDS             complement(8472..8663)
                     /locus_tag="ACHBBW_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9152566.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHBBW_000859"
     CDS             complement(8684..9574)
                     /locus_tag="ACHBBW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9152567.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHBBW_000860"
     CDS             complement(9561..10328)
                     /locus_tag="ACHBBW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9152568.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHBBW_000861"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHBBW_000861"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9152569.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHBBW_000862"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHBBW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9152570.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHBBW_000863"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHBBW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9152571.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHBBW_000864"
     CDS             14899..15282
                     /locus_tag="ACHBBW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152572.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHBBW_000865"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHBBW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9152573.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHBBW_000866"
     CDS             complement(16226..17044)
                     /locus_tag="ACHBBW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9152574.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHBBW_000867"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHBBW_000867"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9152575.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHBBW_000868"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHBBW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9152576.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHBBW_000869"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHBBW_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9152577.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHBBW_000870"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHBBW_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9152578.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHBBW_000871"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHBBW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9152579.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHBBW_000872"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHBBW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9152580.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHBBW_000873"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHBBW_000873"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9152581.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHBBW_000874"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHBBW_000874"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9152582.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHBBW_000875"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHBBW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9152583.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHBBW_000876"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHBBW_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9152584.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHBBW_000877"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHBBW_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9152585.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHBBW_000878"
     CDS             28352..30325
                     /locus_tag="ACHBBW_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9152586.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHBBW_000879"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHBBW_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9152587.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHBBW_000880"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHBBW_000880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9152588.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHBBW_000881"
     CDS             33204..34496
                     /locus_tag="ACHBBW_000881"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9152589.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10565 a         6735 c         6854 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DAYYKC010000017        34075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000017 DAYYKC010000000
VERSION     DAYYKC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.017"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1260)
                     /locus_tag="ACHBBW_000882"
     CDS             complement(28..1260)
                     /locus_tag="ACHBBW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9152590.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1515..2084
                     /locus_tag="ACHBBW_000883"
     CDS             1515..2084
                     /locus_tag="ACHBBW_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152591.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2262..2702)
                     /locus_tag="ACHBBW_000884"
     CDS             complement(2262..2702)
                     /locus_tag="ACHBBW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9152592.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2860..3624
                     /gene="map"
                     /locus_tag="ACHBBW_000885"
     CDS             2860..3624
                     /gene="map"
                     /locus_tag="ACHBBW_000885"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9152593.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3656..4579
                     /locus_tag="ACHBBW_000886"
     CDS             3656..4579
                     /locus_tag="ACHBBW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9152594.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4804..5043)
                     /locus_tag="ACHBBW_000887"
     CDS             complement(4804..5043)
                     /locus_tag="ACHBBW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152595.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5048..5467)
                     /locus_tag="ACHBBW_000888"
     CDS             complement(5048..5467)
                     /locus_tag="ACHBBW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9152596.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5694..6932)
                     /gene="mef(H)"
                     /locus_tag="ACHBBW_000889"
                     /gene_synonym="mefH"
     CDS             complement(5694..6932)
                     /gene="mef(H)"
                     /locus_tag="ACHBBW_000889"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9152597.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7039..7674)
                     /locus_tag="ACHBBW_000890"
     CDS             complement(7039..7674)
                     /locus_tag="ACHBBW_000890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152598.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7788..7880)
                     /locus_tag="ACHBBW_000891"
                     /pseudo
     CDS             complement(7788..7880)
                     /locus_tag="ACHBBW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8016..8651)
                     /locus_tag="ACHBBW_000892"
     CDS             complement(8016..8651)
                     /locus_tag="ACHBBW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9152599.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9163..10305
                     /locus_tag="ACHBBW_000893"
     CDS             9163..10305
                     /locus_tag="ACHBBW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9152600.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10298..10504
                     /locus_tag="ACHBBW_000894"
     CDS             10298..10504
                     /locus_tag="ACHBBW_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9152601.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10583..11776
                     /locus_tag="ACHBBW_000895"
     CDS             10583..11776
                     /locus_tag="ACHBBW_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9152602.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11981..13120
                     /locus_tag="ACHBBW_000896"
     CDS             11981..13120
                     /locus_tag="ACHBBW_000896"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9152603.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13113..13913
                     /locus_tag="ACHBBW_000897"
     CDS             13113..13913
                     /locus_tag="ACHBBW_000897"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9152604.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13913..14740
                     /locus_tag="ACHBBW_000898"
     CDS             13913..14740
                     /locus_tag="ACHBBW_000898"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9152605.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14745..15452
                     /locus_tag="ACHBBW_000899"
     CDS             14745..15452
                     /locus_tag="ACHBBW_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9152606.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15552..16418
                     /gene="rfbA"
                     /locus_tag="ACHBBW_000900"
     CDS             15552..16418
                     /gene="rfbA"
                     /locus_tag="ACHBBW_000900"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9152607.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16432..17004
                     /gene="rfbC"
                     /locus_tag="ACHBBW_000901"
     CDS             16432..17004
                     /gene="rfbC"
                     /locus_tag="ACHBBW_000901"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9152608.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17026..18054
                     /gene="rfbB"
                     /locus_tag="ACHBBW_000902"
     CDS             17026..18054
                     /gene="rfbB"
                     /locus_tag="ACHBBW_000902"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9152609.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18152..19003
                     /gene="rfbD"
                     /locus_tag="ACHBBW_000903"
     CDS             18152..19003
                     /gene="rfbD"
                     /locus_tag="ACHBBW_000903"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9152610.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19037..21070
                     /locus_tag="ACHBBW_000904"
     CDS             19037..21070
                     /locus_tag="ACHBBW_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152611.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21114..22394
                     /locus_tag="ACHBBW_000905"
     CDS             21114..22394
                     /locus_tag="ACHBBW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9152612.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22604..23410
                     /locus_tag="ACHBBW_000906"
     CDS             22604..23410
                     /locus_tag="ACHBBW_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152613.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23422..24642
                     /locus_tag="ACHBBW_000907"
     CDS             23422..24642
                     /locus_tag="ACHBBW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152614.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24632..26218
                     /locus_tag="ACHBBW_000908"
     CDS             24632..26218
                     /locus_tag="ACHBBW_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9152615.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26257..28395
                     /locus_tag="ACHBBW_000909"
     CDS             26257..28395
                     /locus_tag="ACHBBW_000909"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9152616.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28763..29746)
                     /locus_tag="ACHBBW_000910"
     CDS             complement(28763..29746)
                     /locus_tag="ACHBBW_000910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9152617.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30124..31515
                     /locus_tag="ACHBBW_000911"
     CDS             30124..31515
                     /locus_tag="ACHBBW_000911"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9152618.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31531..32571
                     /locus_tag="ACHBBW_000912"
     CDS             31531..32571
                     /locus_tag="ACHBBW_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9152619.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32590..33675
                     /locus_tag="ACHBBW_000913"
     CDS             32590..33675
                     /locus_tag="ACHBBW_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9152620.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33708..>34075
                     /locus_tag="ACHBBW_000914"
     CDS             33708..>34075
                     /locus_tag="ACHBBW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9152621.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGI"
BASE COUNT        11049 a         5830 c         6715 g        10481 t
ORIGIN      
        1 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
       61 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
      121 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
      181 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
      241 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
      301 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
      361 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
      421 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
      481 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
      541 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
      601 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
      661 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
      721 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
      781 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
      841 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
      901 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
      961 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
     1021 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
     1081 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
     1141 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
     1201 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
     1261 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
     1321 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
     1381 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
     1441 atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     1501 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     1561 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     1621 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     1681 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     1741 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     1801 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     1861 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     1921 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     1981 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2041 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2101 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2161 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     2221 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     2281 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     2341 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     2401 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     2461 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     2521 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     2581 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     2641 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     2701 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     2761 aaaagaaaat aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     2821 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     2881 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     2941 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3001 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3061 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3121 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3181 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     3241 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     3301 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     3361 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     3421 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     3481 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     3541 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     3601 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     3661 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     3721 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     3781 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     3841 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     3901 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     3961 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4021 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4081 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4141 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4201 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     4261 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     4321 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     4381 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     4441 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     4501 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
     4561 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttatt
     4621 tgaaatttta tatctaattt tcaattatcc gcataaacac tatacattca agcattttga
     4681 agtatagaaa aacactcatc ttaccacgaa tttaccacga ttgaatgagc cttgatatga
     4741 ccgtaaaact actaagggag atagcacgaa actgtgtatc tcccgatttt tttatacagt
     4801 tgattacttc actccgattt caaatttcaa tattgctttc atcatgtctg tatgaatttc
     4861 tccttttagt tcttcatcaa cctgcataat gattttacca ctttcagagt ggtaaaaagg
     4921 acgcaagcat aatttagaaa tataaccgtc ataaaacttc aaaatctctt taactgccat
     4981 ttcatctccg cttgacgctt tgcaaatcaa ttcaaaagag ggatagccat aatctttact
     5041 catttcttta cttcctttcg ctcaaaagca tttttaactt ccttagtcct ctagttcttc
     5101 tactgcaaat agcagaacgt gatacatggt acagttctga aatttctctg tcgttcattc
     5161 cttgaaaata acgtaataaa atcgcactac gctgtttttc tgacagtcta tgtaaaactg
     5221 taccaagtgt ttcatcagat acataaattg taaagtgcaa gactttaaat gatacacaat
     5281 cataaaaaca ggtatcaatc gttccacgtt caacttgttt cgtttcggct atttcacaaa
     5341 acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt tcgacttttc actttgttct
     5401 taatgacaat cagcattaaa gaattgaact gtaatctgac gatatgctcg aaagaagatg
     5461 tcctcataat ctcaccccct ttctgctaat gctgaaatat ataaacggaa taccgtttat
     5521 accctttaga aatacagaaa aatggtaaga aaaaagccca cacaaaacat atatttgtgc
     5581 gagccttaaa tactgcaata ttactctttc ttttataaca ctatcctttt agcagatttc
     5641 aaattttctt ttatatattg tttttagcct aacatactat ttaacccctt gtattatatc
     5701 tctaactttt gaaaaattct ttttgaatat attgaaatca tgattgaagc aatagaagct
     5761 acaaacataa caacccatgt ttcttgtata aatacatcga ataaaatacc gtaaaccatt
     5821 tgtccaatag gttgaccgca catagcaact gctaccagcg tagccattat ctttcctact
     5881 aattgcggtg gtgtttgtgt ctgaacaaga gtgtaaattt gaacaacaaa catagtagat
     5941 acacccatag ccacaaagca catcactgta actacccagt aggaaactat tgcaggcaat
     6001 tctaaaaata gcgaaattcc cataagtaaa acagaaacag aacaaataag taacaaggta
     6061 taaaggtttt ttagttttaa ctttgaagca acaagagctg ttaaaatacc accacataaa
     6121 ccacctaatg ataatgctga ttgtgaaaaa ccatatagtg tatctggcat ttttaaaact
     6181 tcagaaatta agataggcag accaacaatt aaaacagcac ttaaaaccaa attgaaaatg
     6241 caaaccaaaa agattacgga tataaaaatg ggtttctcct ttttcaaaaa ggtataacta
     6301 tcttttaaat cgtttttagc aactgcaatt acacctactt cacgaggtct tttttggtgt
     6361 ggaatatgga taaaaatttc cataatggct gaaaaagtaa aacatataat actaacaatt
     6421 aaaatgggat tgataccata aaattgaaaa actactccgc ttatagctgg tccgataaag
     6481 tttgctaaag tatttacttg atttattata gcattaccga cggtaagttt atctgatgat
     6541 accaatagag gaatacttgc ttgtaccgct ggttgatatg caccagaaat accataaagt
     6601 aacatcaaag caacaataaa caaaggtatg ataggaattt ttcccaatga caaataaaat
     6661 actaaaatta tgattgctgt tacaaaatct agtatgacca taatgtttct tttatttgtc
     6721 ctgtcagcaa taataccacc taccagcgac aaaataacca taggtaaaaa agagcaggct
     6781 gtagcaatac caaacaaagc tgatgaacca gtttctttta acaaatagta cggtaatgca
     6841 aatcgtagga tagcgtttcc aaataaagaa attatctgtc ctataacaac aagtataaaa
     6901 tcacgattaa aaagtttttt ttctgcttgc ataaaatctc tcctttttat ttaatcaaac
     6961 agaccgtcgg tttatataat ggtttaaata aactgctctt ttatagagca gtctatcgta
     7021 tagggataac aaatacagtt atttacaaaa ctgtataaat gcagaaattg tttcttcgtc
     7081 taataatgtg tctatgccta ttccatttaa aagggcattg aatgtattgc aacgtttttc
     7141 cataccctct ttatccgtca tttcaactaa tgggttgagc catacattac ttaataccat
     7201 gataacttct gcaatttcat gtgaattttc aatctccaaa gacttatcct tgataccttg
     7261 ttctaaaacg ggttcaacaa attcaggtac tacaatttca tacatttgtt ctatttgcat
     7321 agcaagaaaa cgaggattgt ttaatagttg aggagaaact gtcaataaaa cgctttggtt
     7381 agaattgaat accgctgtct taaatatttc tcttaacttt tctaaaccgt ttaaactatc
     7441 gttatctcta attttagcaa atattactgc attttcattt ccaattcttt gaaaaattgc
     7501 ttttaaaata tcttcctttg aatcgaaatg atgataaata gcacctttag aaagttttgt
     7561 ttcattgata atatcttgca atgatgtgtt atcatatcct ttttcacaaa acatttttgt
     7621 agaaacatct aaaatgagtt ttactgtttc ttctggatat ttatttcgtg ccataactta
     7681 cctccaatac aaaccgttgg tctgattaaa gtatatctta gtattttatg cttgtcaata
     7741 taaagacttg caattttata aaaatgcaga atgcaagcct ttttcattta ttctacaatc
     7801 ttccagttgc tttccttatg cggtgtcaat tcatactgtg atacctgcgt tgcctttgcc
     7861 tgatttacca caaatcagat tttatccctt tcttcctctt gattgcccac aacagaaaag
     7921 aaaaagccct gtcaagagct ttgccaccat tggtggtgct ttgcactctt gatcgggctt
     7981 tttgtttgct gtatcttctc gcaagaggaa aaaagttact cgtcctcatc atcaaaattg
     8041 ttattcctta atacttctcg taacagttcc atatcttctt ttgagttcgg gtttatcatt
     8101 ttcacgactt gataaggtgt cttatactta tgtccctcta tgctttcccc aaacatatcc
     8161 atactgtata aatcatcata gcggtctaat aatttttgaa acttcaaatg ctggataaat
     8221 tctttgattg ggtaaaggtc ggacgcttca atgcttttcc cgttgatata gtctgtaata
     8281 tattcccgta acttttctaa ctcatattcc agccattctt cctcgctgtc aaagaagtta
     8341 tcatagtgtc catgtttcag ttctgcattt agacgttctt cggctcttaa aaactgacag
     8401 acttcctttt cagattgatt gacatacttc cattctcccg acaacaattc attgggcgta
     8461 acgtccagca cttccattat cttttccagc gtttcatagg caggatagtt caaccctctt
     8521 tcaatcttgg aaaggctctg catattgata ccgattttgt ccgcaagttc ctgttgtttc
     8581 attcccctaa attttcttat ggtctgtata ttatttccta aatggcttat tttcattgca
     8641 taaccctcca tatctcgtta tatcttcgct attataagtc tatcatgctt aatatgattt
     8701 gtaaaggcaa aaaaagatat tgacaaataa gcatataaga attactatgt tcttgtaatc
     8761 gaataagcac aatagaatga aatataaaaa cattcaaaaa gaaataccta tctgattaaa
     8821 tccgtagata tgaatatcgg ttgaaatgga acttgttaga aaaggcaaaa cagaacttgc
     8881 tgacaaagta tttctgacac ggtggcggaa gcctctcagg cttaccgcca tgacaccttg
     8941 tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc gagccttgtc tgtgcccccg
     9001 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac
     9061 ctgtgagtag taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg
     9121 tttacaggta tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt
     9181 gcacttgaaa tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt
     9241 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc
     9301 aaggaaacac agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt
     9361 tatctgctcg ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc
     9421 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
     9481 tataaggagc attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
     9541 ggcgtatttt tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca
     9601 cagaacaggg actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga
     9661 cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa
     9721 aaatacagaa atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga
     9781 aaattaagcg gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc
     9841 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
     9901 gatattatca accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa
     9961 gaactgttat tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg
    10021 gaatttcctg actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa
    10081 atgaaccctg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
    10141 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg
    10201 attgatgaat gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg
    10261 gacatatcag acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc
    10321 cgtatgggaa aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga
    10381 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
    10441 caaccgcata cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat
    10501 ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc
    10561 aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct
    10621 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
    10681 gggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
    10741 aaaacgggaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
    10801 cggtatcgac agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
    10861 acagaggaca aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt
    10921 aaaagaggac aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga
    10981 atgggcgtta cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg
    11041 ctcgttaaaa gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt
    11101 tgatttcaag ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga
    11161 ggaacaggag caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta
    11221 tgatgatgtg ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac
    11281 agtagccgac attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag
    11341 taaggaacag ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc
    11401 attaagcaaa gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa
    11461 gtcgatagag gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa
    11521 agttgccatt gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca
    11581 caaggacaat cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag
    11641 gctggcaagc aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat
    11701 cagtatcaca atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca
    11761 gcgtctaatc gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata
    11821 agaagataga cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata
    11881 aaataaggct ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa
    11941 tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat
    12001 ggttataaga ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat
    12061 catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc
    12121 aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt
    12181 attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat
    12241 catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg
    12301 gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac
    12361 gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg
    12421 gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg
    12481 gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc
    12541 tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc
    12601 ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt
    12661 tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc
    12721 tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa
    12781 agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag
    12841 tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc
    12901 tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat tgattattgc
    12961 catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca
    13021 gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca
    13081 gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc
    13141 ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt
    13201 caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg
    13261 agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg
    13321 atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc gattctcgct
    13381 atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct
    13441 tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg
    13501 gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg
    13561 tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag
    13621 gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg
    13681 atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag
    13741 acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt
    13801 atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt
    13861 ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc
    13921 agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg
    13981 accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc
    14041 tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca
    14101 aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg
    14161 ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg aactattgcg
    14221 taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg
    14281 acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt
    14341 tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa
    14401 atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg
    14461 gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga
    14521 acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt
    14581 acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg
    14641 acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg
    14701 gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta
    14761 tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc
    14821 tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata
    14881 tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag
    14941 gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg
    15001 cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc
    15061 agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag
    15121 agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga
    15181 taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt
    15241 tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga
    15301 agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg
    15361 agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg
    15421 cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc
    15481 catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag
    15541 agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt
    15601 taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc
    15661 caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag
    15721 atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat
    15781 atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta
    15841 ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt
    15901 ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg
    15961 tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga
    16021 ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg
    16081 acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga
    16141 ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac
    16201 gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta
    16261 tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc
    16321 gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc
    16381 aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta
    16441 acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc
    16501 ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac
    16561 tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc
    16621 caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat
    16681 gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta
    16741 tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact
    16801 ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat
    16861 gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga
    16921 gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat
    16981 ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt
    17041 gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat
    17101 ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg
    17161 gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta
    17221 gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac
    17281 gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca
    17341 cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt
    17401 tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa
    17461 ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa agctggttca
    17521 gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt
    17581 tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgaat
    17641 atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc
    17701 catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa
    17761 acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta
    17821 gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc aggacacgac
    17881 ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa gcctgagttt
    17941 acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg
    18001 tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga
    18061 gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt
    18121 ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag gaaatgggca
    18181 actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg tttcaacaga
    18241 tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca cagaattaaa
    18301 acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag aagaagaagc
    18361 aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac ttgcagcaaa
    18421 agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa
    18481 aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa
    18541 attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc
    18601 ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac
    18661 ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt
    18721 ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc
    18781 gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag atacagatgt
    18841 agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc
    18901 agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt ggcaagaagc
    18961 acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt ttatcaaata
    19021 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    19081 agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    19141 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    19201 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    19261 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    19321 tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    19381 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    19441 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    19501 ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag ctctatcgct
    19561 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    19621 gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt atttctacaa
    19681 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    19741 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    19801 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    19861 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    19921 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    19981 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    20041 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    20101 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    20161 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    20221 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    20281 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    20341 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    20401 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    20461 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    20521 ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    20581 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    20641 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    20701 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    20761 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    20821 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    20881 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    20941 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    21001 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    21061 cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg agagtggaga
    21121 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    21181 tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca actttgttta
    21241 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    21301 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    21361 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    21421 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    21481 tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    21541 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    21601 tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    21661 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    21721 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    21781 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    21841 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    21901 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    21961 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    22021 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    22081 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    22141 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    22201 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    22261 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    22321 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    22381 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    22441 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    22501 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    22561 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    22621 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    22681 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    22741 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    22801 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    22861 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    22921 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccactt
    22981 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    23041 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    23101 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    23161 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    23221 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    23281 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    23341 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    23401 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    23461 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    23521 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    23581 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    23641 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    23701 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    23761 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    23821 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    23881 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    23941 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    24001 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    24061 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    24121 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    24181 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    24241 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    24301 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    24361 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    24421 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    24481 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    24541 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    24601 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    24661 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    24721 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    24781 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    24841 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    24901 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    24961 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    25021 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    25081 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    25141 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    25201 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    25261 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    25321 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    25381 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    25441 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    25501 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    25561 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    25621 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    25681 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    25741 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    25801 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    25861 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    25921 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    25981 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    26041 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    26101 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    26161 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    26221 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    26281 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    26341 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    26401 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    26461 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    26521 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    26581 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    26641 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    26701 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    26761 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    26821 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    26881 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    26941 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    27001 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    27061 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    27121 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    27181 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    27241 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    27301 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    27361 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    27421 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    27481 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    27541 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    27601 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    27661 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    27721 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    27781 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    27841 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    27901 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    27961 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    28021 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    28081 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    28141 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    28201 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    28261 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    28321 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    28381 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    28441 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    28501 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    28561 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    28621 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    28681 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    28741 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    28801 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    28861 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    28921 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    28981 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    29041 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    29101 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    29161 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    29221 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    29281 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    29341 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    29401 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    29461 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    29521 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    29581 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    29641 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    29701 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    29761 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    29821 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    29881 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    29941 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    30001 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    30061 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    30121 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    30181 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    30241 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    30301 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    30361 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    30421 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    30481 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    30541 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    30601 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    30661 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    30721 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    30781 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    30841 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    30901 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    30961 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    31021 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    31081 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    31141 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    31201 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    31261 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    31321 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    31381 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    31441 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    31501 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    31561 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    31621 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    31681 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    31741 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    31801 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    31861 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    31921 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    31981 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    32041 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    32101 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    32161 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    32221 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    32281 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    32341 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    32401 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    32461 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    32521 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    32581 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    32641 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    32701 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    32761 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    32821 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    32881 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    32941 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    33001 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    33061 tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    33121 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    33181 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    33241 ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt
    33301 agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta
    33361 ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct
    33421 ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc
    33481 ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta
    33541 gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata
    33601 acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga gagtatatat
    33661 agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg aaaatatgtt
    33721 atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat cgattgaacg
    33781 aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca tttattgaag
    33841 catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt
    33901 ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca ttacaaaatg
    33961 agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa gataatgagt
    34021 atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga ataga
//
LOCUS       DAYYKC010000018        33603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000018 DAYYKC010000000
VERSION     DAYYKC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.018"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..683
                     /locus_tag="ACHBBW_000915"
     CDS             <1..683
                     /locus_tag="ACHBBW_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152622.1"
                     /translation="KDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFE
                     NFGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNN
                     MIDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQM
                     STEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILY
                     KNTIFTNL"
     gene            716..1393
                     /locus_tag="ACHBBW_000916"
     CDS             716..1393
                     /locus_tag="ACHBBW_000916"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM9152623.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            1881..2846
                     /locus_tag="ACHBBW_000917"
     CDS             1881..2846
                     /locus_tag="ACHBBW_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM9152624.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            2868..3080
                     /locus_tag="ACHBBW_000918"
     CDS             2868..3080
                     /locus_tag="ACHBBW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9152625.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3155..3631
                     /locus_tag="ACHBBW_000919"
     CDS             3155..3631
                     /locus_tag="ACHBBW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM9152626.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3628..5436
                     /locus_tag="ACHBBW_000920"
     CDS             3628..5436
                     /locus_tag="ACHBBW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9152627.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5513..5728
                     /locus_tag="ACHBBW_000921"
     CDS             5513..5728
                     /locus_tag="ACHBBW_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM9152628.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5820..6683
                     /locus_tag="ACHBBW_000922"
     CDS             5820..6683
                     /locus_tag="ACHBBW_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM9152629.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6720..7256
                     /locus_tag="ACHBBW_000923"
     CDS             6720..7256
                     /locus_tag="ACHBBW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM9152630.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            7270..7692
                     /locus_tag="ACHBBW_000924"
     CDS             7270..7692
                     /locus_tag="ACHBBW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9152631.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7622..10021
                     /locus_tag="ACHBBW_000925"
     CDS             7622..10021
                     /locus_tag="ACHBBW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9152632.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10053..12044
                     /locus_tag="ACHBBW_000926"
     CDS             10053..12044
                     /locus_tag="ACHBBW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM9152633.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12067..12318
                     /locus_tag="ACHBBW_000927"
     CDS             12067..12318
                     /locus_tag="ACHBBW_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM9152634.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12308..13537
                     /locus_tag="ACHBBW_000928"
     CDS             12308..13537
                     /locus_tag="ACHBBW_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM9152635.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13534..15615
                     /locus_tag="ACHBBW_000929"
     CDS             13534..15615
                     /locus_tag="ACHBBW_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9152636.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15764..19684
                     /locus_tag="ACHBBW_000930"
     CDS             15764..19684
                     /locus_tag="ACHBBW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9152637.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19685..20629
                     /locus_tag="ACHBBW_000931"
     CDS             19685..20629
                     /locus_tag="ACHBBW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152638.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20633..20836
                     /locus_tag="ACHBBW_000932"
     CDS             20633..20836
                     /locus_tag="ACHBBW_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9152639.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21136..21372)
                     /locus_tag="ACHBBW_000933"
     CDS             complement(21136..21372)
                     /locus_tag="ACHBBW_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152640.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21555..21926)
                     /locus_tag="ACHBBW_000934"
     CDS             complement(21555..21926)
                     /locus_tag="ACHBBW_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9152641.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21923..23251)
                     /locus_tag="ACHBBW_000935"
     CDS             complement(21923..23251)
                     /locus_tag="ACHBBW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9152642.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23212..23541)
                     /gene="mobC"
                     /locus_tag="ACHBBW_000936"
     CDS             complement(23212..23541)
                     /gene="mobC"
                     /locus_tag="ACHBBW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9152643.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23799..24169)
                     /locus_tag="ACHBBW_000937"
                     /pseudo
     CDS             complement(23799..24169)
                     /locus_tag="ACHBBW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            24314..24499
                     /locus_tag="ACHBBW_000938"
     CDS             24314..24499
                     /locus_tag="ACHBBW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9152644.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24657..24824
                     /locus_tag="ACHBBW_000939"
     CDS             24657..24824
                     /locus_tag="ACHBBW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152645.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            25056..25229
                     /locus_tag="ACHBBW_000940"
     CDS             25056..25229
                     /locus_tag="ACHBBW_000940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152646.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            25442..26101
                     /gene="vanR-B"
                     /locus_tag="ACHBBW_000941"
     CDS             25442..26101
                     /gene="vanR-B"
                     /locus_tag="ACHBBW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9152647.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26104..27465
                     /gene="vanS-B"
                     /locus_tag="ACHBBW_000942"
     CDS             26104..27465
                     /gene="vanS-B"
                     /locus_tag="ACHBBW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9152648.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(27556..28857)
                     /locus_tag="ACHBBW_000943"
     CDS             complement(27556..28857)
                     /locus_tag="ACHBBW_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9152649.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            29136..29942
                     /locus_tag="ACHBBW_000944"
     CDS             29136..29942
                     /locus_tag="ACHBBW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9152650.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            29960..30667
                     /locus_tag="ACHBBW_000945"
     CDS             29960..30667
                     /locus_tag="ACHBBW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9152651.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            30664..31635
                     /locus_tag="ACHBBW_000946"
     CDS             30664..31635
                     /locus_tag="ACHBBW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9152652.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            31628..32656
                     /gene="vanB"
                     /locus_tag="ACHBBW_000947"
     CDS             31628..32656
                     /gene="vanB"
                     /locus_tag="ACHBBW_000947"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9152653.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            32662..33270
                     /locus_tag="ACHBBW_000948"
     CDS             32662..33270
                     /locus_tag="ACHBBW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9152654.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(33336..33509)
                     /locus_tag="ACHBBW_000949"
     CDS             complement(33336..33509)
                     /locus_tag="ACHBBW_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152655.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         8706 a         8379 c         8926 g         7592 t
ORIGIN      
        1 ctaaggacag caaaattcta gatgcagacg aaattattaa agattggttt ccaactataa
       61 aagcagatat ttttatatct cactctcatg ctgatgaaaa attagctata cgttttgcat
      121 catggctatt tgaaaatttt ggtcttactg catttattga ttcttcggtt tggggataca
      181 gttctgattt attaaaaaaa atagaccaaa aatattgcta taaggaacaa acaaaaacat
      241 atgattatga taaaagaaat gtaacaacta gtcatgttca tatgatgctt tctacagctt
      301 taaataatat gattgatagt acagaatgtc tgttttttct taatacacct aattcaatta
      361 gtttgagcaa tgaaatcact aatgaacaga aatttacgta ttcgccatgg ctatatagcg
      421 aactgacgac agcttcaata gtggaaaaaa agaatccacg attggaaagt aatcctcaaa
      481 tgagcaccga ggatgttaga agcattataa agcactattc ttcgcaagaa aatctgcaaa
      541 taaaatattc tttaaattta agtgaattaa cttatttacg aaaagctgat ttgaaaaaat
      601 gggctttaga tgctgtggaa tgcactaaag gaagtaatac attagatatt ttatataaaa
      661 acaccatttt cactaatctc taatcaattt actgaatcga aaggaaccgg gtagaatgga
      721 taagaaagtt ttaataaaga ggttctctga agctattgaa aaaggaaatg cagcaatatt
      781 tgcaggtgca ggattatcga tgtcacaagg atatgtaagt tggccggaac tacttaatga
      841 tcctgcaaca gaaatcgggt tggattctaa aaaagaaaca gacttagtta ctctggctca
      901 atattataaa aatgaaaatg gtggatcaag aggaattttg aatcagatac ttatggataa
      961 ttttggtgaa gaattagaaa tatctgaaaa tcatcgaata ctagcaagtt tacctattga
     1021 aacttattgg acaactaatt atgatcatct aatagaaaaa tcaatccgag aagcttataa
     1081 aaaccctcag gttaagaaaa attatactca gttagcaaca actaatccaa atgtggatac
     1141 aattgtttat aaaatgcatg gtgatataga tgatgtcagt tcgacagtaa taacaagaga
     1201 tgattatgaa aaatatgatg attcgtacgc gttatttaaa gaaactttaa aaggagattt
     1261 gttaacaaaa acttttctct ttttaggatt tagttttaca gaccctaatt tagaaagaat
     1321 tttatcagat ataagatggg ttttgagaga aaaccaaaga cctcattatt gtataatgag
     1381 aaaaatatta tagaaaaaat tttaggttat cgctgaaagc cccggaaata cgggcatttc
     1441 gcaggatatg agaactcaat ttactaccaa tttactactt tcggattgag ccgcatatat
     1501 gctgaaccat actgctaata cccgccaccc caaatacggc acatcataaa ttgaatacga
     1561 caccgtaaac gcggcgttcc tctttcccgt catgggagaa caggacgccg cttttttatg
     1621 cccaaataga gaaaggaggc gacgccatgc tatttacttc cggccctgac cgggcgtttg
     1681 aacggctgat gcaggaaaag cccggtttcg atcactacga cggcggcggg gcggacgcgc
     1741 cggaggattg cggcacctgc cgattttacc gcccggattg gaaatatcaa ttctgccagt
     1801 ttgcagagtg tccctatcag ccgggcaagc tgaccgacta tgcagcggtc aaattcacag
     1861 tgaaaggagc gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg
     1921 tgatgtcaaa tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt
     1981 cgcagatgtt gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca
     2041 accgggaaag catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata
     2101 tccggcgtga gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct
     2161 acgaacagcc ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt
     2221 tggaaaatcc gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag
     2281 tacaaagaac tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt
     2341 ccattcctat cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc
     2401 cggaacggaa aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg
     2461 agaacatcga gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg
     2521 aaatcgtaga cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg
     2581 gggacgacta ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata
     2641 tccgctttgt ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat
     2701 acctgcgggc tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg
     2761 tcgcgcacga catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt
     2821 cctgcggcga gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg
     2881 cattgatttt tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt
     2941 acggcggcct gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca
     3001 cccgcattga aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg
     3061 gcctgcgggt gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac
     3121 gccgcttttg actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac
     3181 cattgccctg tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa
     3241 aaagtacctg caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct
     3301 aaagcagctt aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat
     3361 tggagcgttc aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa
     3421 gaccacccag ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga
     3481 tctggcgttt cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg
     3541 caaggttttg tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga
     3601 gaaaatcaag gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg
     3661 aacctcccgt atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg
     3721 gccgctggtg ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct
     3781 tttgccaacc ctctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc
     3841 gtgatcgtgc ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc
     3901 atggagtatg gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc
     3961 aagtttgaaa ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc
     4021 aaagacccca agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg
     4081 aaaacgagat ttttcgtgaa acccaacctg atgcaatgct ttccgaccac ggactatccg
     4141 acttcgtttg tggtgacaga tccgaaaggc actttggttc tggaaaccgg caggatgttc
     4201 cagcaggcgg gctaccgcat taaaatcctg aataccatta acttttccaa gtccatgaag
     4261 tacaacccct ttgtctacat ccattcggaa aaggacgtgc taaagctggt aaataccctg
     4321 atttccaaca ccaagggcga gggggaaaag tcagcggagg atttttgggt gaagtcggaa
     4381 cggttgtttt attcggccct gatcggctat atctggtacg aggccccagc ggaggaaatg
     4441 aacttctcaa cgctgctgga aatgataaac gccagtgaag cccgcgagga cgacccggag
     4501 tttcaaagcc ccgtcgatct catgtttgag cgtttggagc agaaagaccc cgaccatttt
     4561 gcggtgcgcc agtacaagaa attcctgctg tccgcaggca agacccgttc ttccatcctg
     4621 atttcatgcg gtgccagact tgcgcccttt gacatccggg aggtacgcga gctgatggag
     4681 gacgacgaaa tggagctgga caccatcggg gacgaaaaga cggtgctgta tctcattatg
     4741 agcgatacgg acacgacctt taatttcatt ctggctatgc tccaaagcca gctaatcaac
     4801 ctgctttgtg accgggctga tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg
     4861 attttggatg aatttgccaa cattggccaa atacccaatt tcgataaact cattgccacc
     4921 attcgaagcc gtgaaatatc ggcctctatt attttgcaga gccagtcgca gctaaaagcg
     4981 atttacaagg acgccgccga gatcatttcg gataactgcg atagtgtgct tttccttagt
     5041 gggcgcggca aaaatgccaa ggagatttcc gatgcgctgg ggaaagaaac gattgacagt
     5101 ttcaacacca gcgagaaccg gggaagccaa acgtcccacg gattgaacta tcaaaaatta
     5161 ggaaaggagt tgatgtccca agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg
     5221 cagctacggg gtgtgcgccc gtttttatcg gagaaattcg atattaccaa gcatccccgg
     5281 tacaaatacc ttgccgacgc cgacaagaag aatacctttg acgtggacag atttttgact
     5341 atcacgcgcc ggaaacggca gcaagtggta acgcaggatg aagtattcga cctgtatgaa
     5401 atcgacctgt cggacgagga cgccgccgct gaataaacag cggctttttt cattccaaca
     5461 acaaaatcaa aaaatcatta tgagccggaa accggcagaa agaggtaaat ttatggcatt
     5521 ttttaattcc gcagttacta ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg
     5581 cgtatggggc ggcatcaatc tgttggaggg atacgggcag gacaatccgg gggcaaaatc
     5641 gcagggtgtc aagcagcttg tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt
     5701 tcccctgctg tccgggctgc tgggctgatc ctggcctttg tccctgtcca aacccttaac
     5761 taatcgcctc cgcgtcctcc cggccagacc gggagggcgc gggaaaggag gtgctacccg
     5821 tgattagtga aatcattgaa gaatggataa aaggcatcct catggacggt atcacgggca
     5881 acctgtccgg gctgttcgat acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg
     5941 gacgaacccc gcaggcatgg aacagcggta ttttcaatat gctgcacagc ctgtcggaaa
     6001 ccgtgatcgt ccccatagcc ggggccatac tcgcccttgt catgtgctat gagcttatcc
     6061 agatgataac ggaaaagaac aatatgcacg actttgacag ctccatgttc ttccgctgga
     6121 tattcaaatc cgcgtttgca atcctgatcg taacgaacac atggaacatc gtcatgggcg
     6181 tattcgacgc cacccaaagc gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat
     6241 ccattgattt taacaccctg ataccagacc ttgaaagcca attggaggct atgagcgtgg
     6301 gcgctctttt ggggctgtgg ttccaaaccc ttgttgtggg cctcaccatg aatatccttt
     6361 ccatctgcat tttccttgtg acctatggcc gcatggtgga aatctatgtt gtcacgtcgc
     6421 tggggcctat cccccttgcc acaatgggca acgcggaatg gcgcggcatg ggccaagggt
     6481 atctgaaatc cctgctggcg ctgggcttcc aagccttttt aatcatggtt gtggtgggta
     6541 tctacgcggt gctgatacag aatatcggtt ccgcttccga catatccggc gcaatctggg
     6601 gctgtatggg ctatacggtg ctgctgtgct tctgcctgtt caagaccggc agtatatcaa
     6661 aggccgtatt cggggcgcat tgatcccaaa atccaagcat acacaggagg aacttgttta
     6721 tttcaacgta caacatcatt tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac
     6781 agggcgcagc ggaaaatcac tattccacca tgagcattga cgagctgtgc gccctgccgg
     6841 tggaacggct ggcgtccaaa gactgcctgc tgtttttgtg ggccacattc cccatgctgc
     6901 cggaagctct gcggttaatc aaagcgtggg gcttttcatt caagaccgtg gcttttgtat
     6961 ggctgaaaca gaaccgtaaa agccttacat ggttttacgg gctgggccgc tggacaaggg
     7021 gaaacgcgga aatctgcctg ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg
     7081 tccatcagtt tattatcagc ccgattgaac aacacagcaa aaagccggat gtgacccgtg
     7141 aaaaaatcgt agagctggcg ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa
     7201 cccccggctg ggacgtgtgg gggaacgagg tggaatgtga ccttatcctg acgtgaaagg
     7261 aggtgccaaa tggcttatgt aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt
     7321 gtgtttgggc ttaccaaacg gcaactgatc tgtttttccg gggcgctggt gatcggcgtc
     7381 cccttatatt ttctcacccg tgactatctt accaacagcg cggcggccct gctcatggta
     7441 tttgccatgc tgccgggctt tttgcttgcc atgtacgagc gtcacggcca gccccttgaa
     7501 gtggtgatta tgcagatcat ccagtgctgc ttcatccagc caaaggagcg tccctatcag
     7561 accgacaaca cctacaccgc ccttgtgcgg caataccaga tggaacagga ggtaaaggcc
     7621 attgtccaaa aaggaaatcc caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg
     7681 aaaagaaatt gacgcggtaa tccgtaaata taagggcgac ggcaggcccc acacggcgca
     7741 gcagagcatc ccctacgagg tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt
     7801 cttttccaag tgcattgagt ttgcggacat cagctatcag cttgcccagc cggacacgca
     7861 gacggctatc tttgaaaaac tgtgcgacct gtataactat gtggacgcct ccattcatat
     7921 ccagttttct ttcctcaatc ggaaagttga cccggtgcag tacgccaaga gctttgagat
     7981 cgcgccgcag ggggacgact tcgacgacat ccgggccgag tacaccggca ttctgcaaaa
     8041 gcagcttgca aacggcaaca acggcatggt aaagaccaaa tacctgacat tcaccatcga
     8101 ggccgaaagc gtgaaagcgg cgcgagccag attgaagcgc atcggctttg acctgttagg
     8161 ctatttcaag agcatggggg ccgtagccca tgtgatggac ggctgggaac ggctgaacct
     8221 gctgcatggc gtgtaccacc cggacggcga gatattcaat tttgactgga agtggctggc
     8281 cccgtccggc ctctccacca aggattttat tgccccgtcc tccctgtgct tcggcaatgc
     8341 aaagacattc ggcatgggcg gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc
     8401 ggagctgtcg gacgatatgc tggccgactt cctcaatacg gaaagcggtg ttttagtcaa
     8461 tctccatgtg caggccattg aacagaccaa ggcgatcaag accatcaagc gcaaaattac
     8521 cgaccttgac gctatgaaga ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat
     8581 ggacattctt cccagcgacc ttgccaccta cggcgaggac gccaagaagc tgctgacaaa
     8641 gttgcagacc cgcaatgaac ggctgttcca gttgacgttc cttgtcctca acgtggccga
     8701 caccaagcaa aagctgaata acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa
     8761 ctgtcccctt gtccggctgg actaccagca ggaacagggc cttgcaagca gcctgccgct
     8821 gggcgtcaat cagatcaaga tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt
     8881 cccctttgtg acgcaggagc tttttcaggg cggcgcggcc atgtattacg gcatcaacgc
     8941 caagtcccgc aacatgatta tgctggacag gaagcaggcc cgttgcccca atgccttgaa
     9001 gctcggcacc cccggcagcg gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt
     9061 cttaacgacg cctgacgata tttttatttc agacccggag gccgaatatt acccccttgt
     9121 caaacggctc cacggacagg taatccggct gtcgcctacc agcaaggact ttgtgaaccc
     9181 gctggacatc aacctgaatt attcggagga cgataacccg ctggccctga aatccgactt
     9241 tgtgctgtcg ttctgtgagc ttgtcatggg cggcaagaac gggctggaag ccattgaaaa
     9301 gaccgtgata gaccgggctg tgcgcgtgat ctaccggccc tatctggcag acccccgccc
     9361 ggagaatatg ccgatcctgt ccgacctcca caaagcgttg ttagatcagc atgtcccgga
     9421 ggctgaccgg gtggcgcagg cccttgacct gtatgtgtcc ggctccctga acgtatttaa
     9481 ccaccgtacc aacgtggaca tcggcaaccg gcttgtgtcc tttgacatta aggagctggg
     9541 caagcagctc aaaaagctgg ggatgctcat tgtccaagac cagatatggg ggcgcgtcac
     9601 ggccaaccga agccaaggaa aggccacatg gtattttgcc gacgagttcc atttgctttt
     9661 gaaagaggaa cagacggcgg cctattctgc ggagatttgg aagcggttta ggaaatgggg
     9721 cggcattcca acaggtgcca cccagaacgt caaagacctg ctttcctccc cggaaattga
     9781 aaatatcctt gaaaacagcg acttcattac gctgctcaat caggcgtccg gggatagaaa
     9841 aatccttgcg gaacgcttga atttgtccac cgaacagcag aagtacatcg acaattccga
     9901 acccggcgag ggccttttga tctttgaaaa cgtggtgctg ccctttacca accccatccc
     9961 gcacaacacc cagctttaca agattatgac gacccgctta aacgaggtgg cgggagtatg
    10021 acccggcgac gccacaaagg aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag
    10081 tgacgcaggc catgacccgc gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg
    10141 agaacatttc cagccgggag gccgaacagg atttgtccgc gccggggccg ggcggcaccg
    10201 ccgataagct gctggaacgg gccgacgcca tccatgacgg ccataaggcc aagaaagccg
    10261 cgaagcaggc cggggctgtg gccgccgagg gggacgctgc cctgcaccgg ccatccgccc
    10321 ggctccaact gtcggaggaa gaacgggccg acccggattt acagccctat atccgaaaat
    10381 cggacaaacg ggccgataag ctggataagg cccgcgccgc catccccaag aagcgcgtcc
    10441 cggtcaagga aaccgtcttt gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg
    10501 acaaacagga caaggggcct ccctccctga aacccaatcc ggcaggccgt cctgtttccg
    10561 aggtttttct attcggccac gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg
    10621 agggcggcca taagggggag gaacttgccg agcggcaggc cggaaaagcc atccgcagca
    10681 ccatccggca ccacaggctg aaaccctact gggacgcgga aaaggccgag cgcaggctat
    10741 tgtctgccaa cgcggagtat ttctaccaga aatctttgcg ggataacccg gagatcgcac
    10801 aggcggccag caaccccatt tcccggttct ggcagaaaca gcagatcaag cgccagtacg
    10861 ccaaggccgc ccggacagcg ggccagaccg caggcgcggc ggcacagggc gcggccaaga
    10921 cagccggggc ctccacgaaa gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct
    10981 cctttgtggc ccgccactgg aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc
    11041 tgcttatcat cggcggctta cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca
    11101 tcacggcgtc ctcttacttt tccgaggata gcgatatgtt ggccgccgag gacgcctacg
    11161 cggagctgga agccgagctg caagactatc tggacaacta cgaggccacc catgactatg
    11221 acgaatacca ttttgacctt gacgagatca gccatgaccc ctatgtgctg atttccattc
    11281 tgtcggcgct ccatgacggg atatttacca tcggggaggt acagggtgat ttgtccatgc
    11341 tctttgacaa gcaatatatc ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg
    11401 aaaccgatac atggacggac gcggagggca acacccatac agacacctac caagtgccgt
    11461 atgactatta catctgctac gtcacgctgg aaaacttcga cctgtcccat gtgccagtct
    11521 atatcatgga cgaggaaacg ctaagtttct attccctgta tatgtccacg ctaggaaacc
    11581 ggcctgatct gttttccggc agccagtacc ccaacgcctc caccctgacg ccgccgacct
    11641 actacgacgt acccccggag gctttggagg atgccacctt tgccgccatg ctcaaagagg
    11701 cggaaaaata tatcggttat ccctatgtgt ggggcggcag ctctcccagc accagctttg
    11761 attgcagcgg cttcatttcg tgggtaatca atcatagtgg atggaacgtg ggacggctgg
    11821 gggcgcaggg cctatataac atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg
    11881 accttgtatt tttcaaggga acctacgaca cccccggcgt gtcccacgtc ggcctgtatg
    11941 tgggcaacaa tgtgatgctg cattgcggga acccgatttc ctacacgaac cttacttcaa
    12001 actactggca atcccatttt tacagttacg ggcgtttacc ttaacgccag aaaggagctt
    12061 tcaattatgg caaccaaact tgaccgcatc gaaaaggaca tccagaaaac caagtctaag
    12121 atcgcggagt ttcagaaaca gcttcgggag ctggaaacgc agaaaaccga acaggagaat
    12181 ttgcagatca tccagcttgt gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc
    12241 ttacgcagcg gcgcaatgca ggccgcccct gccgcaaccc cttaccatga acaggaggac
    12301 aatgcccatg aagaataaga ccagacggat ttcccgtatt ctccccgcac ttctggccgt
    12361 gatgctctgc gtcacggcct ttcccgttac cgcccttgca ggcggcaatg acccggcacc
    12421 ggccccgctg ccggaggcta caaccgaacc cgctacgggc ggtttggagc ctgaaacaga
    12481 cgaaaccggc accgccacgg acgggacaga aaccgaggcc gaaaaagata ccggccctct
    12541 gaccggcaag gacatcaccg acctgttttc cgcgctgttc ggttccaagg tgagcattgc
    12601 cgccacggac gacggcatcc agatcaccac cggcaccgac aaaaaagaac ctgaacagac
    12661 cggcaccgtc accaccaacg gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa
    12721 gaccgcgttc acccagcttc ccaatgggac gacggttgag gtagtcggca cagacggcga
    12781 ctggattaaa attctcttgc cggagcgcat cggctatgtc cattccgact atatgaccgt
    12841 cagcgaaaag gaagtagcgg ctaccggcga gggcggtttt tccctctcca ttgacccgga
    12901 ggaaattgct tctctgctgg aactgttcaa tggcggcggg gccagcgccg ccctgtcccc
    12961 ggacgggaac ctgtcactga ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt
    13021 tatcaccgtg gaaaccaaaa acggcaatgt gttttatctg attattgacc gggacgacga
    13081 gggaaaagaa actgtccatt tcttaaatca ggtggatgaa gccgatctgc tgacgctgat
    13141 ggaggacggg gaaaccgtca cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc
    13201 cggggctgtg aacacgaatt gcccggtatg taaaaccaac ctgaccgagt gcagcggccc
    13261 ggagccgcag gaaccccagc cggaggaacc cgaagccccg gaggaagaac ccaagggcgg
    13321 cgcgggcggc ctgatcgtgt tcctgctggt ggcgctggcg ggcggcggtg cggcccttta
    13381 ctatttcaag ttcaaaaagc ccaaggctga aaccaccggc agcgacgatt tgggcgagta
    13441 tgattttggg gaggacgagg atttggagga cgaggaaccc gaaaccgagt ttgacccgga
    13501 agctgatttt gtgagtgaac aggaggacga ggaatgacat ataaacttgt aatcgcggaa
    13561 aaacccagcg tggcccgcag cattgcgggc gtgatcgggg ccacggagaa acacgacggc
    13621 tatttggagg ggaacggcta tctggtgagc tggtgcatcg gacatctggt gtcctttgcc
    13681 gacgccggac gctatgacga gcgtttcaaa aaatggcgct atgaggattt gcccattctc
    13741 ccggaaacat ggcagtacat cgtccccgac gacaagaaac agcagtttga cgttctctgt
    13801 tcgctgatgc agcgccccga cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag
    13861 ggggaattga ttttccgttt cgtctaccag atggcgggat gccaaaagcc ctttgagcgc
    13921 ctttggattt cctctatgga ggacagtgct attaaggacg gctttgccca cctgaaaccg
    13981 ggcggcgact atgatccgct gtatcaatcg gccctgtgcc gggccaaggc cgactggctg
    14041 atcggcatta acgccacccg gcttttttcc gtcctgtacc acaagaacct gactgtgggc
    14101 cgcgtccaga cgccgacctt aaaaatgctg gtggacaggg acgccaagat taccgatttc
    14161 aagaaagaga aatatcacat cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc
    14221 cgcttttccg acgctgccga ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc
    14281 gtttgcgttt ccgtgacgcg ggagaaaaag acggaacagc cgcccaagct ctatgacctt
    14341 accaccttgc agcgggaggc taaccggctt ttcggctata ccgccaagca gacccttgac
    14401 tatgcccagg cactgtatct aaagaagctg ctgacctatc cgagaacgga tagtcaatat
    14461 cttaccgacg atatgctgcc cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg
    14521 ccgtttgcca agggcctgaa cctttccccg cagtttgacc gcatcctgaa cagcaaaaag
    14581 gtgtccgacc accacgccat tatccccacc gctgaatttg tgaagcaggg ctttggcact
    14641 ctggccgaga gtgaaaaaaa gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg
    14701 gccgggccgc atatctatga ggccgtcacc gccacattta cctgtgccgg gcaggagttt
    14761 accgccaagg gcaagaccgt cctttccccc ggctggaaag agattgaacg ccggttccgt
    14821 tcttccttaa agaccgacgc cgacgaggac gccgaggcgg tgcgggagct gccggagctt
    14881 tgcgagggcc agacctttgc agacgtggcc gcctctgtca ccgagcattt caccacgccg
    14941 cccaagccct acaccgaaga tacgcttttg tccgctatgg aacgggccgg ggccgaggat
    15001 atgccggagg atgccgagcg caagggatta ggcacaccgg ccacccgcgc cgccattctg
    15061 gaaaagctgg tgcagatggg ctttgtccag agaaaaggca agcagctcat tcccaccaag
    15121 gacggcatca atctggctgt ggtgctgccg gaggcgctta cttctcccca gcttactgcc
    15181 gaatgggaaa accggctgac cgagatcgcc aaggggcagg ccgatcctga cgaatttatg
    15241 gacggcatcg agacgcaggc gcgggagctg gtgcagacct attcctgtat cagtgaggaa
    15301 aagcagaaat tgtttcaggc cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag
    15361 gctgtctatg agggcaagaa aaactactac tgcggaaacc gggcctgcca gttcgtcatg
    15421 tggaagaatg accgattttt tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct
    15481 gcgctgctca aaaccggcaa agtcaaggta aagggcctct attccgtaaa gaccggcaaa
    15541 acctacgacg ggacggtgct tttggctgat accgggggca agtacgtcaa ctaccgcatt
    15601 gaacacagga gctaaaggac agtaacacag caagcatagg gagtgtggct acactcccgg
    15661 taaaatcccc cggccgcgtc cctgccgggc cggggatttt tgcttgttgg ggaagtcccc
    15721 aaccccctat tttttgaaac agaacagaga aaggagtgac cgcgtgaacg aagtattttc
    15781 tatccaagtc cataaccggc agtttttgga acagggcaaa caaggcgtct ggctggattt
    15841 gcccaccaca accgaaacgc tgcaagccgc cctgcgggat gttggcatta ctgccgacaa
    15901 tccgcaggat ttttttatca acgggtattc ctgcccggag gacagacacc ttgccattcc
    15961 ctatgacatg gtgctggccg ccgacgtgga cgagctgaat ttccttgccg cccggctggg
    16021 ccagcttgac gccgccgaga tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg
    16081 ctttgagaat atcggccaga tcatcgacta caccgaaaat gtggattact atgtgcatct
    16141 tcccgacgtt aggggggatg gacagttggg cgactactac ctgaaccgtt ccggcatggt
    16201 ggatatgccg gaggaatgga aagccgggat tgatacggcg cagtttggac gccacattgc
    16261 ccagcaggaa caaggcgttt ttacccagta cggctatctg gtgagaagcg gcgacgaatg
    16321 gcagcgcgtc catgagggcc agccggtgcc ggaggaatac cgggtgatgc actaccccca
    16381 gccggaagct gaccgggagg aaccgcaggc ccagcagccg gaggcatccg aaagccccga
    16441 caaagcgccg cccccggtta tcccgatcat gctggacggc atgaacagca aagaacgcat
    16501 gaaagtgatt actgaccgtc tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta
    16561 tatggcttac ttatccgcta tgtccaagat gcacgattac agtctgaaca atacgctgct
    16621 gatcgccatg cagggcggtt cgattgtggc gggctataac aggtggcagg atgaatttga
    16681 ccgccatgtg aagcggggcg agaaaggcat taagatactg gccccggccc cgttcaaggt
    16741 taagcagcag atggagaaaa ccgacccggc cacccagcag cccgtgatcg gcgcggacgg
    16801 caagcccgtg accgaagaac aggaagtgac ggttccggcc tttaaggtgg tgacagtctt
    16861 tgacatcagc cagaccgagg gcaaggattt tcccatgccc tatatcagtg aacttaccgg
    16921 cgacgtgcag cagtatcagg atttctttgc cgcgctggaa cggacatcac cctatcccat
    16981 aagcattgag ccgatttcag acggggccaa gggccgctgc ttctacgcaa aacagcgcat
    17041 tgccatcaac gagggcatga gcgaattgca gaccctaaag accgccatcc atgagattgc
    17101 ccatgccaag ctccacgcca ttgaccggga ggcccccgcc gaggaacagg cggcccggcc
    17161 agaccgcagc acccgcgagg tacaggccga aagcgtggcc tataccgtct gccagtatta
    17221 cgggctggac acgtcggact attcttttgg ctatgtggcc gattggagca gcggcaggga
    17281 gttatccgag ctgaaagcct cccttgaaac catccgggcc acggccagcg aattgataac
    17341 cgagatcgac ggccattttg cccagcttca gcaggagcgc gaggcccggc aggaacagga
    17401 ggccgtcacg gttgacgtta ccccggaggc agcggccccg gcgcaggccg aggaagaaca
    17461 ggccccggcc cttgatccgg cagccgagcc gcttgtgact gtgctatgga gcgaaagccc
    17521 ccagctaaag gacggccagc aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct
    17581 ggatgcagca tatccggcag aggcaggcta tgacaaaacc gcgttccgca ttgactgtat
    17641 ctttcagggc gagccggaca gctacgaggg gcggcaggat tttggagata gggacggttc
    17701 cctgatcgaa catatccaga gctaccacca attttacgcg caggacgaaa actggaaaaa
    17761 gcatgtaatc gctacaaccg gcatagaggg ctgggagcag gacaaggccc atcgggatat
    17821 gctgttaggc gagtttgtcc cctatatgaa gctgcattgc agccttgccc aaatggaaca
    17881 gacggcgcag ggccgcttac agtccgggga aaccctgacg ccggaggaaa cggcctattt
    17941 gggggcgctt ctctcttatg tgcaggagtg ccgccccatg ctcaatcagg gccagtatca
    18001 gttgccggag ccgccgaaac tggctgactt cgaccagagc ttgcaggact acaaagcaca
    18061 ggtacaggcc gagatcgcgc aggaggccgc cgcagccggt atgaccgtgg aggaatacgc
    18121 ggcggctggc tatgaagcac cccaacagga caccttttcc atctaccagc ttcgaggcgg
    18181 ggaggaaacc cgcgactatc ggtttgagcc atacgagcgt ttacaggccg cagggctggc
    18241 cgtggacagt gccaactatg accttgttta tacggcaccg ctggataaaa gcacgtcttt
    18301 ggaggatatt taccgcacat tcaacatcga ccacccggca gattttaggg gacacagcct
    18361 ttccgtttcc gacgtggtgg tgctgcacca gaatggacag gacaccgccc actatgtaga
    18421 cagcttcggc tatatggaag tgccggagtt tttgcgggaa aatcccctgc gtaccgccga
    18481 actgtctacg gagcagaacg aaaacatgat tgacggcatt atcaacaata cgccgcccga
    18541 accgcagccg gaggaacccg aacagctatc ttttgaggat gtggcagttg cttccggcca
    18601 gacacaggag gaacccccgg aggctgcgcc cccgcaaacg gctgtccgct attatcccat
    18661 caatgaggga gccgcccgcc gcgccaagga agccatgagc ttttctgact accggcccgg
    18721 cagcgccacg gcggaatacc ggcactatgt cgatcaggcc gtggaaattg cggagcgcca
    18781 gaaaaagcgt gtggagccag agtatcacga aaagatagac agcctccttg acacctacgc
    18841 ccgcaagctg gccgagaaca tgaataaggg ctatgagatt accgcccgcg tcccctctgt
    18901 cctgattgcc gggccgtcca atttcccggt acgggcgaaa gaaaagcaga acgccgccag
    18961 tgaccgcaac atggaggaat ttcagtacat tcagggcttg cttgacaaaa tccgcagcac
    19021 cggcatgggt ggtatcagcg ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact
    19081 ggaaaagctg gaagcgtcgc aggagttgat gaaggccgtc aatgcctact accgcaagca
    19141 tgggacgctg gacggatgcc cccatctgat cgaaagaggc attgaaaacc tgaaagcgga
    19201 tatggcgtcg ggctggcact atgagaaaaa accgttccag tcctggcagc tctccaacaa
    19261 taacgccgag atacgccggt taaagggccg cattgaggaa ttgacccgcc aaaaggaggc
    19321 ggcctatgtg ggatgggagt ttgacggcgg cactgtggag atcaaccggg tggccaaccg
    19381 cctgcaaatc ttctttgagg ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa
    19441 cggtttccgc tggtcgccca aggccggggc atggcagcgg cagttaaacg acaccacaat
    19501 ccgcgtcgct gaccgtatca agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt
    19561 acagcttgcg gcccgcaggg agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc
    19621 caagaccgaa caggccaagc agccaccggc gcgggaaaaa tcaaagggat tggaggtaga
    19681 gtaaatgcag aagtttgaaa atgtggacgt tctggccgcg ctggaacaga tcatgcggca
    19741 gaacaccgcg ttttatcaaa gtgactttgg cattgacaaa gacattatca ggcgggatgc
    19801 cgccagcgat caggccgcag acaagaccct gctctggatg tcccggccct ccgggacgta
    19861 ctgtttccgg gaacgtgatg ttttcctgaa agacacccga cagtacaaca catggaaatt
    19921 ttacggggag cagacccgtg acaagatact ggcctacgcg gtggagctga ccggcacaca
    19981 gggcggcact atccggggca atctctatga gctggactat ccgcagcatt tccggcacgt
    20041 cattgaaacc gcccagccgg tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga
    20101 catcccggag agccagcgtt ttgacggaag ccctgaccgg gtattgggaa actttttgta
    20161 ctatgaggcc cagccccatg acccggctgt gctgcaggag gcgctacgga cggagcagca
    20221 cagccggggg cgggccgtcc ccggcgattt caaagtccac gtcaccgctt tgcatgacag
    20281 ccggattgaa accgaggccc ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa
    20341 cagccccaat aaaacgcatt tcatggtgga gatctccccg cactttgcgg cgattgcttc
    20401 caccaaggat aatgaccggc tctttgccat gctgccctat aaatccctgt gttttaccgg
    20461 gatgaaaggc aggcatggcg tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag
    20521 tattcgcagg ccccggccct ctatccgcaa gcagctatcc gacagcaaac aggccgccag
    20581 cccaaaaaag gcggcggcca gaaccaaaaa gaatgaattg gaggtataag gaatgatgca
    20641 ctttaccgtg gaggaagaaa acctgatctg tatgtaccac aatgcagacc ggcgcagaac
    20701 gataaacagg ctcaccgccg tcatgccggt tatggatggg gatatgcggg cgcaggccca
    20761 gcagaccctt tccaagctgg aacgcatgac ggatgccgac tatgacggcc agaagttcca
    20821 cttcacagac gaataacgaa cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt
    20881 ccggcgtctt ttctttttgc ctattcctac gcatttagaa cccggtattg tcggggatag
    20941 gatgaagtat tcgcttgccc ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc
    21001 aatcccttaa atgcgtaggt tgcagctata attagacccc tgaaacagat atagccgcgc
    21061 agcgggtgta tctgtttcag gggtgtgcag gtgatagccg caaagcggcg taagagggtg
    21121 cagccctctt gccgtctatc tctgttccag cgggaagctc ggcaatgctt ccagcagttt
    21181 cagggaaatg cgctgccgta tatcttcgtc aatgactttc ttctttttgc cgtttgcctg
    21241 tctgatctcc accgtggaaa gctcgttgat gtagtcagca taatgggcca acactttttc
    21301 cgtggcccac ctctcaccgg caacggcggc tttgatcgtt tcatagtcca gcagcttttc
    21361 ttgtccgtcc atcctgatac ccccgtcaga ctgatttttt ctatgatacc atattcggaa
    21421 gcatggaacc agagaaaacg gcgcttttct gctggtaaat tctacgcatt tagaacagca
    21481 cttcggcggg gataggataa aacacccaca taccccgctg accatgccgg aaaagccttg
    21541 aaatataagg gcttttacag ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc
    21601 tcggcctctt tgcggcggcg catccgtttg gagcaggccg cgcagtattt ggcccggtta
    21661 gacttcggcg tgaacgtggc cccgcactca cagcagcgtt tcacttcgtc ccggtctttg
    21721 gtgatctcgg catacagggc cgcatccagc gggaggacag cggccttaaa ccagcggcac
    21781 agaagcgaat aggaaatgct ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg
    21841 cagttgccgt cacaatagtt gcagcactca tgcgtcagct tccgggcctt gcgatattga
    21901 gggtaattca ttcgtggaat actcatagct cttgcgtcca ttcctttccc ggcgtcatac
    21961 gcaaaatgcc gtccacattc tgcttgatga tgccgtattc ctgcatctgc tttttcgctt
    22021 cgccgtactt ctcatacagc cgttcctttt cctcggccaa tcgcttgtac tcggctttca
    22081 gggcgtacag atcggggagc tttttcacac cctgtccctt tagggccttt gccgcagctt
    22141 catacaaaat caacgtgctt tcatgctcac gccggaattt tgccttatcc gctgcattgc
    22201 ggtattccat agccacgggc cagagctgct tgtaggtaga aatattcttt atcagcaccg
    22261 ccatatccgc aagccggtgt tccatagctt tgagggtagc ggcggcctcg tcgctggcgg
    22321 cggtgatctc ggccactttg ctttccaaat ccgggtaggt gtctatccca tgttcagtca
    22381 ggaaattcaa agtctttgcg gcctgcttca aattgtggag cttcgcccac ctctcatagc
    22441 ctgccgactg ctgggcctta atgttgtttt ccagatcaat ccgcaaagaa atccccttgc
    22501 gctctttggg cgctctggtg ataacgcggc ctttgatacg ctctttgatg gcttcctcgg
    22561 tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag
    22621 aaataaactt tcccggcttg atctcatagc cctgcgcctg catgagccgc aggaaatcat
    22681 caaaatcttt tgcctgcggg atagcggcgt caatggctgc tttgagcttc gctttccagc
    22741 tcgtcccttt ccgctgggcg tcccactcgg catagctctt gcccttactc tggccgggct
    22801 tcaccacgga caatccgttt tccttgcaca gcctgtcgct ggtgcgccgg atataggcat
    22861 agctctgttt gttggaaatg tacttgcgct gatttaccat atccacggca cagaaaataa
    22921 tgtgattgtg gacgtggccc ttatcaatat gggtagtcag cacataggga tatttccctt
    22981 gcagcacttc ctcggcaagc tgcttcccga tctcatgggc ctgttccggc gtggcttcgc
    23041 cgggggcaaa ggattgtatc aggtggtggc ccagattatt gcccttttgg tatgcctgcg
    23101 ccagcagcat ttcaaattca atgtccgctg tttcataaga gcaggcaaag gaggccacca
    23161 gcagcttgcc gtcagttttg tccgggttct cgatgtagtc aagggctttt ttcagcgttg
    23221 acttgatagg atgtatcttt gtaactgcca tatctgcgcc agcgccccct ttatatcctc
    23281 gatgtcctgt gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt
    23341 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
    23401 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
    23461 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
    23521 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
    23581 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
    23641 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
    23701 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
    23761 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
    23821 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
    23881 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
    23941 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
    24001 acaaccggca gactgggaat ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa
    24061 tatttatttt ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca
    24121 catccgcgcc gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc
    24181 cacttacatt gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa
    24241 agtttatatt tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct
    24301 gtggaggtgt actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc
    24361 cccacgatgg ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc
    24421 gacgatctgg ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg
    24481 aaaggcggtt actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga
    24541 gagaaagcat ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca
    24601 gaccgataaa aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga
    24661 agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg
    24721 ctgctgtatc agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg
    24781 aagtctccag acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat
    24841 tttaataccg gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat
    24901 tattacattt cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg
    24961 ctgattttct actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag
    25021 agcgaccgga gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca
    25081 ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg
    25141 agaacgccgc aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat
    25201 tgcttgccag tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt
    25261 aagaaggtgt taattattat tatcttgatt taattcgttt aaaccaataa tatagataca
    25321 tacaccatga aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg
    25381 ctttgaaact acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt
    25441 tatgtcgata cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc
    25501 gtttctggct gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac
    25561 caagttttac gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa
    25621 cgggcatgaa cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac
    25681 agccctgtcg gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt
    25741 aacaaagcca ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag
    25801 cggtgcgctg gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac
    25861 ggtactttgt gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct
    25921 gctggtgcag aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg
    25981 atatgacttt gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa
    26041 gctgccggaa aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata
    26101 ataatggaaa gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta
    26161 ctgcttgtcg gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg
    26221 atggaattac agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc
    26281 gataggcttg atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag
    26341 ttttatattg aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca
    26401 aatagtttta gacccaactt tctttatgtg gtacatagag atgataatat ttcgattgtt
    26461 gctcaaagca aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt
    26521 atgattgcga taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg
    26581 acaacgccga tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta
    26641 ccgccgccgc tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg
    26701 tatgtcaggc tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag
    26761 ttggaggaaa cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc
    26821 gcggctacaa gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat
    26881 tctaagtatc tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa
    26941 atactggagc ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac
    27001 atagggcgca cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac
    27061 cagcggttcg tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc
    27121 caaaaggcac tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag
    27181 gtacggatat ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc
    27241 gttcacattg atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag
    27301 gcgcgaagca gaaaaagtgg gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc
    27361 atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat
    27421 ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa
    27481 atgattttgg gctctttgtc aaataggact gatagagtga gataagaaca tctaaggtag
    27541 aattcctttt ctactttaga tgttttttga ttcataggta atcagattat ttagtaagaa
    27601 aattctggtc ttcattttcc gaaagcttcg aaagccataa gcgactcgtt tcaatgcttt
    27661 gatgtgggta tgcagatttt ctattttggc atttgaataa ggcaattcga gggctaaacg
    27721 aatgccggat tcatagttta taaaagcttt ttttatagaa tgcagttctt ttggaacgga
    27781 attgggcatc gttcttacaa gtttaaagaa agtgtccgcc tctttgtcgt gaaacgcttg
    27841 aagaatttct tgatagtaaa aataggcgat tcgtaattcg tcagagatcg aaagcagacg
    27901 atccaccact tcactttcgg tgagatacgc ccaaccaaaa ttgcgccatt tggtccattt
    27961 gtcataaaca agcttgtcct gtcgtttgat cagaagccga gacaacgcct ttaattgtcg
    28021 ataatatttg gcttgtgttg gatcgtttcg atcaaagctt ttcatgattc gtacgcgaat
    28081 atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa cgatccacaa cgagttttgc
    28141 ctttggaaaa cattccttta tcagagaggc ataagaaaca ttcatgtccg tcacgatgac
    28201 ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa taagaaacga gagccttttt
    28261 cttccgtgaa ggaagaatat ccagtaattt tccagtatcg ccatccatac aagaaaagga
    28321 gtagggaccg acttgatttt tcagtgaacg aaattcatcc attaataaac aacgcggtaa
    28381 ccaatttctt tttacttttg tcatggggat cagagaagcg agtgtccgag aaacggtcga
    28441 ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct tgtttttcag ttagctcttc
    28501 taaaatttta tagcgcactt gtttagcgat aaagcagttt ctatcgacaa tgtaggtatt
    28561 cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt tgtttcgcta aagcaagata
    28621 agtcagttgg ttcgcacatt tattaagttg aatgagcgat tttttctttc cgttcttaac
    28681 gatcatgcct tcatgattca tctgtttaca caaaggacag cgacgaattt ctttacttaa
    28741 cgtcgcagaa acaacaagac tcttgatatt cttaatgctc ttcgattcca catttgtgat
    28801 gaaaatgttt ttatctttga ttccaagtag tagtttgata tgatgaagat caggcataca
    28861 gaacgctcct ttgattgtgt tgtggtgatt caattctaac ggagcttctg tatgttttgt
    28921 tttttcatgc tcaaaaggga ccgatagaat gattttctat cagtcctaga aattatagag
    28981 ccgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac
    29041 agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa
    29101 atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca
    29161 atgcggatca tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg
    29221 cgttcattat ttcgttcaca gtctgcacgc tgtttttggg gtggagactg gcttccgtat
    29281 tggaggcaac acagataccg cccatccctg caactcatac aggcagcagc actgacgtag
    29341 tggagaattt ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat
    29401 ggagcctgat tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg
    29461 agcaactatc aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt
    29521 ttgatgccgc aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag
    29581 aaaaacagca agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct
    29641 ctgcacaggc taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc
    29701 agcttggtct tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt
    29761 atagatggct ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca
    29821 agacagagat aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag
    29881 ctgccacaga gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat
    29941 gagaaaagga tgttatgtta tggacagaaa aagattgaca cagcgtttcc cgttcctgct
    30001 tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg
    30061 tcgctatgcg cagacgatag gggaaaaatc gctttcccat ttgctctttg aaacggattg
    30121 cgcattatac aaccacaata ccggatttga catgatatac caggaaaaca aggtgttcaa
    30181 cttaaagctg gcagcaaaga ccttaaacgg cttattgata agaccggggg aaaccttttc
    30241 tttctggttg ttcctgcata cgccattgac aattatccag cgcagaggtc acgaagtaaa
    30301 ggagtttcca gagccaaaca gtgacgagat taaaggggtg gatgcaacca tttcagaggg
    30361 ctggattgat ttaaaagtgc gaaacgatac cgactgcacc taccaaatat gggtgacctt
    30421 agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa gagccccaag cattatacaa
    30481 aattacaaat ggcagtatcc agtatgtccg tgaaagtggc gggatttatg aatatgccca
    30541 ggttaaacgg atgcaagttg ccttaggtac cggggaaata atagattgca agctgcttta
    30601 tacaaacaaa tgcaaaatct gttatcccct cccggaaagt gtggatattc aggaggagaa
    30661 ccaatgagaa aaagtgtggg cattactgtt tttggatgcg aacaggatga ggcaaatgct
    30721 ttccgcgctt tatcgccgga ttttcatatt atccctacgc tgattagcga tgcgatatcg
    30781 gcagacaacg caaaattggc cgctggcaat caatgcgtta gcgtaagcca taagtccgag
    30841 gtttccgagg cgacaattct tgcgctgaga aaggtcgggg taaaatacat ttctacccgc
    30901 agcatcggct gcgatcacat tgatacgact gccgccgaga gaatgggaat ctcggttggc
    30961 acggttgcgt attcgccgga cagcgttgcg gattatgctt tgatgctgat gctgatggcc
    31021 atacggggtg caaaacccac catgcacgcc gtggcgcaac aagatttcag attggatcgt
    31081 atccggggga aagaactggg ggatatgact gtgggagtta ttggaaccgg ccatatcggg
    31141 caagcggtcg tcaaaaggct gcggggattt ggatgccatg tgctggccta tgataacagc
    31201 cgaaaaatgg atgcagatta tgtccagctt gatgagcttc taaaaaacag cgatattgtt
    31261 acgctccatg tgccgctttg tgcggatacc cgccatctga tcggtcagaa gcaaattgga
    31321 gagatgaagc aaggcgcatt tttaatcaac accgggcgcg gagcgcttgt cgataccggg
    31381 gcgctggtgg aggcgttgga aagcggaaag ctgggtggtg cggcactgga tgtgttggag
    31441 ggcgaggatc agtttgttta taccgattgc tcgcagaaag tgcttgacca tccctttttg
    31501 tcgcagcttt taaggatgcc gaatgtgatc atcacacccc atacggcgta ctacactgag
    31561 cgtgtgctgc aggatactac agaaaaaaca atcaggaatt gtctcaattt tgaaaggagt
    31621 ttacagcatg aatagaataa aagtcgcaat catcttcggc ggttgctcgg aggaacatga
    31681 tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt gatacggaaa aattcgatcc
    31741 gcactacatc ggaattacaa aaaacggtgt atggaagcta tgcaagaagc catgtacgga
    31801 atgggaagcc gacagtctcc ccgccatact ctccccggat aggaaaacgc atgggctgct
    31861 tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat gtggctttcc cggttttgca
    31921 tggcaaatgc ggggaggatg gtgcgataca ggggctgttt gtattgtctg gtatccccta
    31981 tgtgggctgt gatattcaaa gctccgcagc ttgcatggac aaatcactgg cctacattct
    32041 tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg attgataaag gtgacaagcc
    32101 ggaggcgggt gcgcttacct accctgtctt tgtgaagccg gcacggtcag gttcgtcctt
    32161 tggcgtaacc aaagtaaacg gtacggaaga acttaacgct gcgatagaag cggcaggaca
    32221 atatgatgga aaaatcttaa ttgagcaagc gatttcgggc tgtgaggtcg ggtgtgcggt
    32281 catggggaac gaggatgatt tgattgtcgg cgaagtggat caaatccggc tgagccacgg
    32341 tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc tcagaaaatg cgatgattac
    32401 agttcccgca gacattccgg tcgaggaacg aaatcgggtg caggaaacgg caaagaaagt
    32461 atatcgggtg cttggatgca gagggcttgc ccgtgttgat ctttttttgc aggaggatgg
    32521 cggcatcgtt ctaaatgagg tcaataccct gcccggtttt acatcgtaca gccgctaccc
    32581 acgtatgatg gccgccgcag gaatcacgct tcctgcactg attgacagcc tgattacatt
    32641 ggcgttaaag aggtgataag tatggaaaat ggttttttgt ttttagatga aatgttgcat
    32701 ggcgtccgtt gggatgccaa gtacgccaca tgggataact tcacaggaaa accggtggat
    32761 gggtatgagg tgaatcgcat catcggcaca aaggccgtgg cgtttgctct gcgcgaggca
    32821 caaatccatg cggctgcgct tggctatggc ttgcttttat gggatggata tcggccaaga
    32881 actgcggtgg actgcttcct gcgttgggca gcgcaaccgg aggacaatct cacaaaagaa
    32941 aaattttacc ccaatataga gcgagccgag ttgattacaa agggttatgt ggcctcacaa
    33001 tccagccata gccgtggaag cgcaattgat cttacgctct accacctgga tacaggggaa
    33061 cttgtttcaa tgggaagtaa cttcgatttt atggacgaac ggtcgcatca tacagcaaaa
    33121 gggatagggg atgcagaggc acaaaatcga agatgcttgc gtaaaatcat ggaaagcagc
    33181 ggatttcagt cttatcgctt tgaatggtgg cactataagt tgattgatga gccatacccc
    33241 gatacctatt ttaattttgc cgtttcataa tgaaagtatt ggttttgtat cagattttgg
    33301 atttgataaa atctatgctg gtcatggaca atcttttact ggaaagtggt taaagcatct
    33361 tttgtaatac attgcaaatt atagtattca atctgcccag cggcagaaaa gaaaaaaacc
    33421 gctgttgggc agacctttcc cacgcccatt ttgtattttg ctggcggcgg ttttgagaaa
    33481 tcaaggccgc cgttcttttc tggcagcaaa aggtgtggcg gcccatagga ggatgccgct
    33541 atgctgtttt gctattgcca tgctctaatc cacttccacc actcaaaaaa agccttgccc
    33601 ctc
//
LOCUS       DAYYKC010000019        33343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000019 DAYYKC010000000
VERSION     DAYYKC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.019"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1757
                     /gene="rny"
                     /locus_tag="ACHBBW_000950"
     CDS             201..1757
                     /gene="rny"
                     /locus_tag="ACHBBW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9152656.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1836..2642)
                     /gene="proC"
                     /locus_tag="ACHBBW_000951"
     CDS             complement(1836..2642)
                     /gene="proC"
                     /locus_tag="ACHBBW_000951"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9152657.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2783..5356
                     /gene="mprF"
                     /locus_tag="ACHBBW_000952"
     CDS             2783..5356
                     /gene="mprF"
                     /locus_tag="ACHBBW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9152658.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5510..6361
                     /gene="ispE"
                     /locus_tag="ACHBBW_000953"
     CDS             5510..6361
                     /gene="ispE"
                     /locus_tag="ACHBBW_000953"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9152659.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6606..7544
                     /locus_tag="ACHBBW_000954"
     CDS             6606..7544
                     /locus_tag="ACHBBW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9152660.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7567..8247
                     /locus_tag="ACHBBW_000955"
     CDS             7567..8247
                     /locus_tag="ACHBBW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9152661.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8220..9044
                     /locus_tag="ACHBBW_000956"
     CDS             8220..9044
                     /locus_tag="ACHBBW_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9152662.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9211..10029
                     /gene="purR"
                     /locus_tag="ACHBBW_000957"
     CDS             9211..10029
                     /gene="purR"
                     /locus_tag="ACHBBW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9152663.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10532..11905
                     /gene="glmU"
                     /locus_tag="ACHBBW_000958"
     CDS             10532..11905
                     /gene="glmU"
                     /locus_tag="ACHBBW_000958"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9152664.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            11999..12970
                     /locus_tag="ACHBBW_000959"
     CDS             11999..12970
                     /locus_tag="ACHBBW_000959"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9152665.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12976..13078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13144..13713
                     /gene="thiT"
                     /locus_tag="ACHBBW_000960"
     CDS             13144..13713
                     /gene="thiT"
                     /locus_tag="ACHBBW_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9152666.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13794..14567)
                     /locus_tag="ACHBBW_000961"
     CDS             complement(13794..14567)
                     /locus_tag="ACHBBW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152667.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14834..16534
                     /locus_tag="ACHBBW_000962"
     CDS             14834..16534
                     /locus_tag="ACHBBW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9152668.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16504..16722
                     /locus_tag="ACHBBW_000963"
     CDS             16504..16722
                     /locus_tag="ACHBBW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9152669.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16742..17632
                     /locus_tag="ACHBBW_000964"
     CDS             16742..17632
                     /locus_tag="ACHBBW_000964"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9152670.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17650..17934
                     /locus_tag="ACHBBW_000965"
     CDS             17650..17934
                     /locus_tag="ACHBBW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152671.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18421..19035
                     /gene="gmk"
                     /locus_tag="ACHBBW_000966"
     CDS             18421..19035
                     /gene="gmk"
                     /locus_tag="ACHBBW_000966"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9152672.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19040..19345
                     /gene="rpoZ"
                     /locus_tag="ACHBBW_000967"
     CDS             19040..19345
                     /gene="rpoZ"
                     /locus_tag="ACHBBW_000967"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9152673.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19598..22006
                     /gene="priA"
                     /locus_tag="ACHBBW_000968"
     CDS             19598..22006
                     /gene="priA"
                     /locus_tag="ACHBBW_000968"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9152674.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22021..22512
                     /gene="def"
                     /locus_tag="ACHBBW_000969"
     CDS             22021..22512
                     /gene="def"
                     /locus_tag="ACHBBW_000969"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9152675.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22505..23443
                     /gene="fmt"
                     /locus_tag="ACHBBW_000970"
     CDS             22505..23443
                     /gene="fmt"
                     /locus_tag="ACHBBW_000970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9152676.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23443..24801
                     /gene="rsmB"
                     /locus_tag="ACHBBW_000971"
     CDS             23443..24801
                     /gene="rsmB"
                     /locus_tag="ACHBBW_000971"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9152677.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24814..25554
                     /locus_tag="ACHBBW_000972"
     CDS             24814..25554
                     /locus_tag="ACHBBW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9152678.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25551..27620
                     /gene="pknB"
                     /locus_tag="ACHBBW_000973"
     CDS             25551..27620
                     /gene="pknB"
                     /locus_tag="ACHBBW_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9152679.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27785..28684
                     /gene="rsgA"
                     /locus_tag="ACHBBW_000974"
     CDS             27785..28684
                     /gene="rsgA"
                     /locus_tag="ACHBBW_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9152680.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28697..29347
                     /gene="rpe"
                     /locus_tag="ACHBBW_000975"
     CDS             28697..29347
                     /gene="rpe"
                     /locus_tag="ACHBBW_000975"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9152681.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29348..29986
                     /locus_tag="ACHBBW_000976"
     CDS             29348..29986
                     /locus_tag="ACHBBW_000976"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9152682.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30141..30995
                     /gene="mreC"
                     /locus_tag="ACHBBW_000977"
     CDS             30141..30995
                     /gene="mreC"
                     /locus_tag="ACHBBW_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9152683.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            30999..31508
                     /gene="mreD"
                     /locus_tag="ACHBBW_000978"
     CDS             30999..31508
                     /gene="mreD"
                     /locus_tag="ACHBBW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9152684.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31763..33337
                     /locus_tag="ACHBBW_000979"
     CDS             31763..33337
                     /locus_tag="ACHBBW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9152685.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        10988 a         5967 c         7218 g         9170 t
ORIGIN      
        1 aaccatcgtt taatgaataa gtagaaagcc gtttctttgt ttatttaaag ttgacactac
       61 ggggtacaaa cattagaata aagttgtgca tgttttttgt acacatatat gcgaagatgc
      121 atattgacga gtttagaata atttatcata gaagctgtat agatgataaa aatgaaaaag
      181 atagtatgga ggtggatttt atggtattca acattctcct cgctatcatc ggtttaattg
      241 tcggtcttgg tttagggttt atggtcgcaa agtcacgtca cgagaaagaa atagctggtg
      301 cacaatcatc agctgctgga atcatcgata gtgcaaaaaa agaagcagaa actctaaaaa
      361 aagaagcatt gttagaagct aaggaagaaa atcagaaata ccgttctgaa gttgaaagtg
      421 agctgagaga aagtaagtta gaattaaaat ctcaagagaa tcgtttgatt caacgcgaac
      481 aaacgctgaa tcgtaaagat gactcgttag aaaaacgcga gaactcactt gaaagtaaag
      541 aagaagcact tagttcaaaa cagcaattaa ttgaagagcg agaaaaagac gttgaaaaaa
      601 tgattgaaga tcaacaaaaa gaactagaaa aaattgctgg tttatcaaaa gaggacgcac
      661 gagatgtcat catgaaatcg actgaagaag agctgaatca tgagttaact ctaatggtaa
      721 aagaatctga acagcgtgcc aaagaagaag cagatcgcaa agcgaaaaac ttattatcat
      781 tagcgattca acgctgcgca gcagatcaag tttcagaaac gactgtttca gtcgttaccc
      841 ttccgaatga tgaaatgaaa ggtcgtatca ttggacgaga aggtcgtaat attcgaacgc
      901 ttgagacgct gactggtatc gatttaatca tcgatgatac accggaagca gttgttcttt
      961 ccggattcga tccaatccga agagaaatcg cacggatgac cttggaaaaa ctgatacaag
     1021 atggacgaat ccatccagcg cgtatcgaag aaatggtcga gaaatctcgt aaagaaatgg
     1081 atgaacgtat tcgcgaatac ggggaagagg cagcgtttga agtcggtgct catacacttc
     1141 atccagatct aatcaagatc atcggacgtc tgcgattcag aactagttat ggacaaaacg
     1201 tcttgaagca ttcggtcgag gttgcaaaat tagctggtat cttagcagca gagcttggcg
     1261 aagatatcca attagcaaaa cgtgccgggt tgctccatga tattgggaaa gcacttgacc
     1321 atgagattga aggatctcac gttgagatcg gtgcggaatt agctgccaag tacaaagaaa
     1381 acccagtagt aatcaacgcg atcgcttctc atcatggaga tgtggaagct acttctgtta
     1441 tctctgtcct tgtagctgca gcagatgcgc tctcagcagc aagacctggt gcgcgaagtg
     1501 aatcattaga aaactacatc cgtcgtttaa gaagtctgga aaatatttcg aacagcttcg
     1561 ccggtgtaga atcaagttac gcagttcaag ctggacgcga agttcgggta atggtcaagc
     1621 cagaagaaat ctcggattta gacgctgttc gtttagtacg tgatattcgt aaacgaatcg
     1681 aagaagaact ggattatcct ggacacatca aagtaatcgt tattcgggaa actcgtgcaa
     1741 ctgattatgc taaataataa aaaatacaaa agaagagccg cggcaaaagc cacagctctt
     1801 ctttttttgt aaggcacgaa aaaacattgc tatcattatt ttttattcaa catctcaaga
     1861 tctttctcga tcgtcgtatt gattcctttg atgattgctg ggatgaatcc tgcttcttct
     1921 aaggcaacta cacctgcaac agttgtaccg ccaggagaag aaacttggtc tacaagttcc
     1981 catggatttt tatcacttga aaggaatttt tgacaagaac ccataattgc ttttgcagca
     2041 atctcagtgg ctgtctgttt ggacaaacca ttcagtacac cagtccgtcc catcgcatcg
     2101 atcagcatgt aaacgaatgc gggtgagctg ccagcaaggc caatgaatgt actgaaatct
     2161 gattctgcta aaggatagac gctgccgatt gcttcaaaaa tcatcgtagc ttgctgaagc
     2221 tgttcctccg tggcagcttc atttttgcaa atcgcagaaa cgcctgctcc tacgctgaca
     2281 ttgacattcg gcatcacacg tacgatggag gcctgaggat cttcaagcgc tgtttctaaa
     2341 tctattaatg attttcctga agcaatagaa atgaataaga ctttctggct atattctctt
     2401 agttcttcca atactttttc aatgattttt ggtttcacgg ctaaaagaac gatatctgat
     2461 tgtgatgcaa cttcctgatt acttttgcat gcatggacgc cagtttcagc agcaaatttt
     2521 tgtgcatttt caaaatgtcc actggaaacg aacacctctt ctttcgagac tgttcctttt
     2581 tttaataaac catcaataat agcttgtgcc atattccctg tgccgataaa tcctactttc
     2641 atgttatcct ctcctttagc attaggatat ctttcttttt ctatcatcgc aagcaaagaa
     2701 caggtctaaa gtggaaaaat taaaataaat aagataacat tggggtatga cacaaaagta
     2761 tacaagttag gaatgggaaa atgtgaaaaa taaacgatac ttgatgctgc aatggataaa
     2821 ggaacatagt ctgattttaa aactgatttt tttcggctcc gtgttagtct ttgtggcaaa
     2881 tcaagtcgca aatattgcta atggcatgag ttggcaagat atttggcaaa ggatgggaca
     2941 acaaagccgc atgacgatcc tgctcatgat tctggctgga ctgctgggtg tcactccgat
     3001 gttgttgtat gattgggtga cgatcagagt cttagaaaaa caaggaaaac caaaaatgga
     3061 aagaaaagag tttcttcttg ctgcgtgggt aacaaacacg atcaacaatc tcgccggttt
     3121 tggtggtgtg gtaggagcaa gtctgcgggc tagtttttat ggaaagcaga caaatagaaa
     3181 aatggtttta gccactgtat caaaagtcgc actatttatg ctgactggtt tgtctgtatg
     3241 gtcgtttctt acatttattg atgtcttttt tattcagtca gaaagtattt ttcgaagcta
     3301 ttggatctgg cttttcggcg gtagtttttt agcaccagtc attttctttt ttgcttatct
     3361 aaagagaaag acattgttct tcgagtttta tccaaaaggc ttgcttggtc tgctactggc
     3421 atcctttgga caatggacgg gagcccttgc ggtatttttg atcatcggca agttgatgag
     3481 aatagatatt tcgatgcttt cgatctatcc tatgttcatg attgctacat tgataggaat
     3541 gctgacgatg gtcccaggtg gaatggggac ttttgacgtt ttgatgatct taggaatgag
     3601 tcaattgtct gttcggcaag atgtagcagt agtttggcta ttatattatc ggttgtttta
     3661 ttatgttcta ccttttatga cgggacttct attgttcatc acacatacag gagtcaaaat
     3721 caatcgattt ttagataaca ttccgcgact gtttttccaa aaggttgcgc atatcgtgct
     3781 cgtaagtgct gtctattttg cggggatcat gatggtactt ctatcaacaa tcactaatct
     3841 gtctgcaatc agtcggctat ttcaagtatt attgccgttc tcattcaatt ttttagatca
     3901 gacagtgaat ctattggttg gctttttatt actcggcctt gctagagcac tttcagtaaa
     3961 agtcaaaaag gcatttctgc ctacgattgc tttactatct tttggtattt taaatacaat
     4021 cacacgtacg ttatcttggc agttgatatt ggtatatgtc ttgattctgt tagctgtgtg
     4081 gttatccaga aaagaatttt atcgaacaaa attcgtttat tcttggggag ctataatctt
     4141 tgatgctagc ttgttcggct ttttgtttat cgtttacgca attgcaggct atcacagcgg
     4201 acgcttttta gtcggtcgaa taacaggaaa tcattttatt ttgtttcctt cagatgatgt
     4261 gtggttctca gggcttattg gtttgggcat ctcattgcta gctttagtgg cgctttatca
     4321 atatttagct gaagatacta cgaagcttgg cagtgagtac gaaaaagacc gttttgaccg
     4381 tttattggaa acatacggag gcacagctac aagccatcag cttcgcttgt caggttacag
     4441 ttatttttat taccaagaag acgaccaaga tcaagtcgtt tttggttatc agatcaaagg
     4501 aaacaaatgc tttgttttag gagatccagt tggcaatcct aaaatgatgc gggcagcaac
     4561 gaatcagttt atgaaacaag cagattgttt aggctatcag cttgcgtttt ataagatatc
     4621 agaaagctac gtagtgatgc ttcatgaatt gggttttcac ttcacgaaag taggtgaagc
     4681 agggattgtt gatctagcaa cagaagactt gtctcggctg agtcagcaac ttgaatatca
     4741 acgtttgatg aaagaaggat accattttac ttggtacgat caactgccgg aagaattaca
     4801 tcaagctgta aatgaagttt cacaaaaatg gcttcaaggt gcacgagaaa aatacttctc
     4861 tgtgggacga tttgataaat catatgtatt atctagcggt gtcggtattg tccgtaaaga
     4921 tcaaaaagtc gttggcttca tcaccgctca gccgatgacg aagaagcagg cgggatatga
     4981 tttgttgcgg gtcttaccta aagaatctga ggaattagct gactatctgt tagccaatct
     5041 gtttattgta tacgagaaaa tgggctactc ggaagccaat ctaggcttag ctccattagc
     5101 taatgttgga gaaacagatt tttctttctt tcaagaaaaa gtcatgcata tcgtatataa
     5161 ttatggtaat tttttctatt ccttccagtc tgcttatgaa aagaaattaa aatatgtcga
     5221 tcattgggaa ggacgttatt ttgcttatag gaaaggaagt aatttcggat ttgctaccat
     5281 gcagctactc agtctgatag gaaaagaaaa aggaaatgtt ccgagtttgg cagaagaagt
     5341 gctcacagaa ttataagaat cactagaact tgtttggatt atgaagagaa aattctgtga
     5401 aagctgttga aacgatgaca aaatcaacca ctaggcagac ataagctata cttttatttt
     5461 tccctatgat agaatacttg cgacttagac ttttcgaatg ggtgagagga tggaattgat
     5521 tgaaaaagcg ccagccaaga taaatttagg actggatgta ttatatagac ggcaagatgg
     5581 gtatcatgag ttggaaatgg tgatgtcgag cgtagacttg gcagatcatc tattttttga
     5641 agaaataaca gaagacaaaa tcattgtaga aacaaataaa gcatttttac cggtagatga
     5701 acggaacaat gtttatcaag ctgctttatt gctaaaaaat aaatacaata tcaaaaccgg
     5761 tgtcaagatc tttgttaaga agaatattcc agtagcagct ggattaggcg gaggaagtac
     5821 agactgtgca gctgctctaa gagggatcaa ccaattatgg aatctgggct gttcgttaga
     5881 agaactggca gaaatcggtt tggaagtcgg tacagatgtt ccttactgtg tctacggaag
     5941 tacggcattt gtcggtggca aaggggaaga aatccaagta ttaccttcta tgccacagtg
     6001 ctgggttgtg ttggtcaaac caagaatgag tgtctctaca cgtacagtgt ttagagaagt
     6061 agatacgcaa gccatccaac atcaagacat ccacggactt gctgaagcca tccgagagca
     6121 agattatcag cagatgatca aaaaaatggg aaacagttta gaagatataa cgggaaagcg
     6181 gcatccagtc atccatcaaa tcaaagagcg tatgatcaag tacggagcag atgctgcatt
     6241 gatgagtgga agcggaccaa ccgtttttgc cttgtgtcaa cattattcaa gagcgcagag
     6301 agtatataat gggttaaaag gtttttgtga ggaagtttat ttagtacgaa ccctgcaata
     6361 aaatataaga agtaaaatag gtgatgaaaa actgaggaat cctaaaatgg tattcttcag
     6421 ttttttattg tagtgaaatg gctagatatg agcagcaact catccatttt acgatttaat
     6481 caagaatata catgcttaaa tcagaaattg atggatttct attgactgtg tttttttatt
     6541 ttgatacatt agttcacgtt aataaatcgt aaaattccga tttgttcatt gaaaggagca
     6601 tataaatgaa aaaatatctt ttgacagcta cggtaatgat aggtgcttta ctgtttgcag
     6661 catgtggaaa tacgaacaag gaagcagaca aaaaagagga tttgacgatc gttacgactt
     6721 tttatccgat gtatgatttt acaaaagaga tcgtagggga tgaaggaaac gtgaaacttt
     6781 tgatccctgc tggaacggag ccgcatgatt tcgaaccaag tgcaaaagaa agagcagaaa
     6841 tttccgatgc agatgttttc gtgtataata gttcagacat ggaatttttt gtggattcgt
     6901 taaaggattc agtggatagc aaacagacct tgatgatcga agcagctaaa ggaatcgacc
     6961 gtttagaatc gcaagaagca gacgaacatg aagaatcaga agaagggcat ggtcattcac
     7021 atgaatacga tccacatgtt tggctcgatc cggtattagc tataaaagaa gtcagaacga
     7081 ttgctgaaga attaggcgaa aaatatccag ataaaaagga aacttttacg aagaatgccg
     7141 atgcttatat caaaaagctt gaagcactgg atcaaaaata ctccgatgag ctaaaagatg
     7201 caacaaaccg cacatttgtc actcagcatg ctgcttttgc ttatttagcg aatcaatacg
     7261 acttaaaaca agtagcgatt tcgggcgttt caccagatca ggaacccaca ccaagtcgct
     7321 tagctgagct aaaagaattt gtgaagaaaa acaacatcaa agtgatttat tttgaagaaa
     7381 atgcctcttc gaaggtagct gagacattgt cgaatgaaac aggagtgaag ctagaagtgt
     7441 tgaatccttt agaaagcttg actaatgaac aaatcaaagc aggcgaaaat tatatttctg
     7501 tcatggaaaa aaatctgaaa gctttaaaag aaagtatcaa ttaagtttta gaatggaggt
     7561 tgtaaggtgc gttatatcga aatatctgat ttgacctttt actatgatga tgaacctgta
     7621 ctagaagatg tctcttatca tgtagatgcg ggtgaatttg tcattttaac tggagaaaat
     7681 ggagcagcca agtcaacctt ggtgaaagca tcgttaggat tactgaaacc ttcaaaagga
     7741 atgataaaga ttgctgagaa aaatcaagaa ggaaaaaaat taagtatcgg ttatattcct
     7801 caacaagttg catctttcaa tgcaggtttt cctagtacgg tgatcgaatt agtccgttca
     7861 ggaagatatc caagaggccg ctggttcaag cgtctctctg aaaaagatca tcaacatgta
     7921 cagcgtgctt tagaagctgt agggatgtgg gatatgcgaa atcggcgaat aggtgaattg
     7981 tctggcggac aaaagcagcg aatcagttta gcgcgtattt ttgctactga ccctgactta
     8041 tttgttcttg atgaaccaac aacaggtatg gatgaagaat ccagaaatga attttatcgt
     8101 cttctccgac atagtgcgca tgtccatggg aaatcagttc taatgatcac ccatgatcat
     8161 gaagatatca aacagtatgc ggacagacag atccgactgg tgagaaagga ggattctcaa
     8221 tggcgttgct ttcatatgaa ttcatgagaa gagcgtttct tgccgctatc ttcattgccg
     8281 gaatcgcccc gatgctaggt gtgtttttag tgatcagaag acaatcgctg atggctgata
     8341 cgctttctca tgtctcttta gcaggggtag cgttaggatt ctttttgaac cttaatccta
     8401 cacttacgac catgctagtc gttgtattag cagctgtgat attggagtat ctgcgaacga
     8461 tctaccgaac ctattcagaa atctccattg ccattttaat ggctggtggt ttggcgctag
     8521 cgttagtatt gatgaatttg agcggaggga attcagcaac aagtatccaa tcctatctgt
     8581 ttggttcgat cgtgaccatt acatgggagc aagtcatctt tttaggaatc ttattcgttg
     8641 ccatcggtat attgttcttt ttattcaaac ggccgatgta tgttctgact tttgatgaag
     8701 ataccgccca cgtggatggg ctacccgttc atttgatgtc gatgatgttc aatgtgatta
     8761 ctggtgtggc tattgctgtg atgatcccga ttgccggtgc acttttgata tccgcaatca
     8821 tggttcttcc cgcagcaatc ggtatgcgat taggcaaaag cttcaatgtc gtgattgcta
     8881 tcagtgtttg tgtaggattt ctagggatgc tgtcaggact aactagctcg ttttatctag
     8941 atacacctcc aggagcaaca atcacattag tctttattgc tttgttcctg attgtaaata
     9001 tcatcaaacg aatattggtt gccttcagaa gaaaaaataa ctaaattata aagaaaagaa
     9061 gccttataaa aacgaaaaat tttttatagg cttcttttta ttttaataaa taccgaacta
     9121 ttttcttatt ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac
     9181 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
     9241 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
     9301 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
     9361 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
     9421 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
     9481 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
     9541 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
     9601 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
     9661 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
     9721 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
     9781 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
     9841 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
     9901 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
     9961 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
    10021 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
    10081 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
    10141 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
    10201 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
    10261 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
    10321 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
    10381 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
    10441 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
    10501 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
    10561 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
    10621 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
    10681 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
    10741 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
    10801 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
    10861 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
    10921 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
    10981 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
    11041 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
    11101 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
    11161 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
    11221 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
    11281 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
    11341 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
    11401 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
    11461 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
    11521 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
    11581 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
    11641 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
    11701 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
    11761 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
    11821 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
    11881 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
    11941 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
    12001 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
    12061 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
    12121 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
    12181 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
    12241 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
    12301 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
    12361 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
    12421 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
    12481 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
    12541 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
    12601 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
    12661 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
    12721 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
    12781 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
    12841 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
    12901 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
    12961 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
    13021 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
    13081 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
    13141 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
    13201 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
    13261 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
    13321 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
    13381 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
    13441 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
    13501 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
    13561 tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg
    13621 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
    13681 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
    13741 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
    13801 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
    13861 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
    13921 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
    13981 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
    14041 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
    14101 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
    14161 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
    14221 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
    14281 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
    14341 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
    14401 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
    14461 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
    14521 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
    14581 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
    14641 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
    14701 ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa
    14761 tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa
    14821 aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc
    14881 gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga
    14941 cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac
    15001 cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga
    15061 aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt
    15121 tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat
    15181 ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac
    15241 agcacttgtt ttaggtagag gaggagcgca cggtgcttat cagattggtg tgtggcaggc
    15301 gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa
    15361 cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc
    15421 gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt
    15481 ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc
    15541 tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa
    15601 aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat
    15661 ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc
    15721 cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa
    15781 aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca
    15841 agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca
    15901 aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt
    15961 acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac
    16021 atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa
    16081 agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa
    16141 tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc
    16201 aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat
    16261 ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag
    16321 tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa
    16381 aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt
    16441 gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca aggaggaaca
    16501 accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac
    16561 aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc
    16621 gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac
    16681 gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg
    16741 aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga
    16801 tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat
    16861 cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg
    16921 aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa
    16981 ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg
    17041 tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt
    17101 cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga
    17161 tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt
    17221 agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag
    17281 ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt
    17341 tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg
    17401 cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact
    17461 tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg
    17521 ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca
    17581 gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag
    17641 gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt
    17701 aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac
    17761 tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg
    17821 aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct
    17881 cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga
    17941 tgctgaacaa agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc
    18001 tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa
    18061 taagtgatgt tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga
    18121 aactattttc acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt
    18181 atacacggcg ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga
    18241 gaagctgttt atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc
    18301 ttggtacagg gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt
    18361 gcagaaaaaa agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc
    18421 atgtcagagc gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca
    18481 gtacgaaaag caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact
    18541 acccgtaaaa tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa
    18601 gagtttgaat caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat
    18661 tattatggaa ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc
    18721 ttggagattg aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc
    18781 atctttttga ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca
    18841 gatgcagatg aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg
    18901 gctctttacg actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa
    18961 aacattattg ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg
    19021 ttaaaggaga tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt
    19081 aaattcaaaa tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag
    19141 tgcacagcct actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga
    19201 aatcgatgca ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa
    19261 gatggaagaa gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg
    19321 tatccgtgaa gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag
    19381 caaacccagc tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg
    19441 tctgaacttt ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt
    19501 tggcagaaga taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg
    19561 cttgataaga aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat
    19621 tgtggacgta cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat
    19681 ggaaacagct ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca
    19741 tgtccaagga tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa
    19801 ggcagttatt cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc
    19861 tgattatatg aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc
    19921 gagtgtgatg aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt
    19981 tcaggtattg tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag
    20041 cctttctcaa ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca
    20101 tacacgaaat aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat
    20161 cgaagaagaa tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg
    20221 tttatctgaa ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac
    20281 tgctgtcttg aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta
    20341 tcgcgaccct tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga
    20401 acaaaaaaat gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt
    20461 tttgctggag ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga
    20521 tgttttgtca gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca
    20581 aatggtcgag cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact
    20641 ttcccaaggt gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt
    20701 agtaggcgca cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga
    20761 tgaagaacat gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct
    20821 agcaatttgg cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc
    20881 tttagaatca agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg
    20941 agccaaagca gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca
    21001 gaatcatcgg acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga
    21061 caaaaaagaa caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg
    21121 tagggactgt ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat
    21181 ggatacacgt tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg
    21241 tcctaattgt ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga
    21301 ggaactccga gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg
    21361 aagaaaaggg gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt
    21421 attagggaca caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt
    21481 gttgaatgct gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca
    21541 attgcttacc caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat
    21601 ccaaacattc aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca
    21661 gttttatcag caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac
    21721 tgtgaaaata accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat
    21781 tgccaatcag ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag
    21841 catgattttg agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga
    21901 accgaatctt cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca
    21961 aggtcttttt gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt
    22021 atgcgctatc ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc
    22081 gatgtaatca cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg
    22141 aatgacggaa tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc
    22201 gaagtagatg aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt
    22261 gaaagccttg acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga
    22321 gcagatgaag tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct
    22381 tttggctatt tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc
    22441 attgagaaaa tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat
    22501 cttgatgact aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg
    22561 gctgatagaa tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg
    22621 gcggaaacga gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg
    22681 tgtgctgcag ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt
    22741 gcctgatgtg atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt
    22801 accaaaatta ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc
    22861 gcctgttcat tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat
    22921 gatcaaaaaa atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca
    22981 agatgatgta gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga
    23041 aacattgccc aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac
    23101 tttttcgcca aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga
    23161 aattgacaac caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg
    23221 catacgctgg aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg
    23281 tacgatcatc cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt
    23341 agccatcaat gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa
    23401 cggaagcggt cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aatccctaaa
    23461 aaaatgaaaa aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt
    23521 ggcgcctact cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa
    23581 gatgtgggtt tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa
    23641 ttttacgttg caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt
    23701 ctttatcttt ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta
    23761 aacgaggcgg tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat
    23821 ggtgtcttga ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct
    23881 atcgaaagac tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat
    23941 caaatcggat acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct
    24001 agtgcccgag tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa
    24061 gaagagatca cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg
    24121 catttagctt ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg
    24181 atgcttgtag cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca
    24241 gcacccggag gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa
    24301 gtggtcgctt tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt
    24361 ctaggtgtct cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag
    24421 ttcgaagcag agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg
    24481 cttcggcgaa aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca
    24541 aaaatccaat tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg
    24601 acttatagca cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg
    24661 gaaagacacc ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca
    24721 atcgaagaga aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt
    24781 tgttgtttgc gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta
    24841 ggaagacgac gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc
    24901 aagcttgctg tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag
    24961 atggcggtaa cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa
    25021 atcgcccaat ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga
    25081 caagaacagc cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat
    25141 gactggttca cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa
    25201 atcatgcaat tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc
    25261 agcgaagaaa tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg
    25321 cctggaacag tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg
    25381 ttatgttcag atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat
    25441 caagaaggcc cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc
    25501 ggagcggata atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt
    25561 tgggcaaaaa attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg
    25621 caaatgtatt tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac
    25681 gatttgactt tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag
    25741 caacagaact cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca
    25801 tgcagtatct ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac
    25861 attatcctgt tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt
    25921 ctttagctca taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg
    25981 ataatgaagg tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat
    26041 cgatcacgca gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca
    26101 gaggaagtat ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga
    26161 tgttgacggg tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt
    26221 ttcaagatga tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa
    26281 acgtgatttt acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa
    26341 tgtctgatga tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc
    26401 ctcatgtgat ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc
    26461 ctgattcatt caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag
    26521 aggaggaaga agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc
    26581 tattagctat tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc
    26641 gaggagaagt tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac
    26701 tcgcaaaagc gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg
    26761 aagaaggtaa tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg
    26821 aagtcactct gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta
    26881 aaagttatga agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc
    26941 aaaaagaaga ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat
    27001 cagaagatac agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac
    27061 cagaaacagt gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc
    27121 ttgttacaca aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg
    27181 taccatcagg ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa
    27241 cttacatcaa ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct
    27301 atacaaaaag cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg
    27361 agacttccga atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg
    27421 caggaacgac aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc
    27481 cagcgtcttc tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca
    27541 gtagcacaag caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca
    27601 cctcagaaag ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat
    27661 ttgttgcatt ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt
    27721 tgagaagttt gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac
    27781 ggaggtggca cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata
    27841 cgcaaacgga gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac
    27901 gccgcttgtc ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct
    27961 ggaaatcttg ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg
    28021 aatcattgtc acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct
    28081 agttaccttg gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc
    28141 agacgaccca gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt
    28201 gattgtaccc aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct
    28261 atcggtcttt atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc
    28321 ggaactgaat ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac
    28381 acgtcatgtg gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag
    28441 ctcgattgac tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt
    28501 agaagcttct gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga
    28561 agtaaaacat agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca
    28621 attcttacaa gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa
    28681 ataggaggaa acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt
    28741 acaaagagac attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat
    28801 ggatggccga tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc
    28861 tgttactaaa ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc
    28921 agcatttgca aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat
    28981 tcatcgggca cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc
    29041 aggaacacca gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat
    29101 gactgtcaat cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac
    29161 ggaactcgct gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg
    29221 aatcgttcca gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg
    29281 atcatatatc tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt
    29341 agactaaatg aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct
    29401 gctgtccaaa tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc
    29461 aggctaccca gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa
    29521 ggaagtattt gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga
    29581 tacacagtta ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat
    29641 tggagctacg ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc
    29701 tcgcttcaaa ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta
    29761 ttatttgcct ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg
    29821 ctgcttgact cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa
    29881 agaagtagaa tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt
    29941 ttctttttca gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata
    30001 aaaaaatttg tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta
    30061 agaggagcaa cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct
    30121 aattaattcg aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct
    30181 tggttattgt tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg
    30241 aaggaaagcc caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa
    30301 caatctctgc cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt
    30361 caacttatgc cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac
    30421 agcaaaacaa gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc
    30481 agacgctgac tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt
    30541 ggcaggacat gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag
    30601 tcatgtcgca aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa
    30661 tcgaattact gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg
    30721 ctaatggcga agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg
    30781 tgacccaatt aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc
    30841 ttggagggaa ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata
    30901 gttatgggct ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag
    30961 ttgtgacgat tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga
    31021 agtactatct gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa
    31081 tgctagggga gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat
    31141 tattatgttg cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg
    31201 gggctatcta tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt
    31261 taattgtatg gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat
    31321 ttttcagtat gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga
    31381 tttttaaatt agcagtagta aataacacgt attttattac aaggttttta ggacctacac
    31441 tgctgttgaa catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg
    31501 atgaataatt tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa
    31561 gaaatgctag acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca
    31621 tttttaaaag ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc
    31681 tatggtacaa tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt
    31741 aagccagtcg gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc
    31801 aatgaccttg actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca
    31861 gatacaacag caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc
    31921 acaaatcgat gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt
    31981 agcaaaacaa gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt
    32041 acaagaacgt attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt
    32101 aagcaacaca agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc
    32161 tattggacgc gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca
    32221 acaaaaacaa gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa
    32281 agaattagct gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca
    32341 agctgatttg aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa
    32401 gaaagctgac ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga
    32461 acaacaacgt ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga
    32521 agcacgcgaa caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag
    32581 ctcagcaaca gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc
    32641 tacacaatct tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac
    32701 agcgcctgaa tcttcagcaa cagaagaatc aacaacagcg cctgaatctt cagcaacaga
    32761 agaatcaaca acagtgcctg aatcttcagc aacagaagaa tcaacaacag tgcctgaatc
    32821 ttcaacaaca gaagaatcaa caacacctgc gcctacaaca ccatcaacag atcaaagtgt
    32881 tgatacagga aacggcacag gaagtagtac tccggctcca acgccaacac caacacctga
    32941 acaaccaaaa cctgtaacac ctgctccagc tccttctgga agtgtgaatg gtgcagcaat
    33001 cgtagcagaa gcttacaaat atattggtac tccatatgtt tggggcggta aagatccaag
    33061 tggctttgac tgctcagggt tcacacgcta tgtttacatg caagtaactg gtcgtgacat
    33121 cggtggttgg acagttcctc aagaatcagc tggaacaaaa atttcagttt ctcaagcaaa
    33181 agctggagac ttgttattct gggggtcaca aggcggaact taccacgtag cgattgcttt
    33241 aggcggagga caatatatcc atgctcctca accaggcgaa agcgtaaaag ttggttcagt
    33301 tcaatggttt gcccctgact ttgctgtcag catgtaagaa tga
//
LOCUS       DAYYKC010000020        32150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000020 DAYYKC010000000
VERSION     DAYYKC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.020"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..468)
                     /gene="yajC"
                     /locus_tag="ACHBBW_000980"
     CDS             complement(88..468)
                     /gene="yajC"
                     /locus_tag="ACHBBW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9152686.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(565..1710)
                     /gene="tgt"
                     /locus_tag="ACHBBW_000981"
     CDS             complement(565..1710)
                     /gene="tgt"
                     /locus_tag="ACHBBW_000981"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9152687.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1928..2626
                     /locus_tag="ACHBBW_000982"
     CDS             1928..2626
                     /locus_tag="ACHBBW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9152688.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2794..5433)
                     /locus_tag="ACHBBW_000983"
     CDS             complement(2794..5433)
                     /locus_tag="ACHBBW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9152689.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6052..7083)
                     /gene="queA"
                     /locus_tag="ACHBBW_000984"
     CDS             complement(6052..7083)
                     /gene="queA"
                     /locus_tag="ACHBBW_000984"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9152690.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7276..8451
                     /locus_tag="ACHBBW_000985"
     CDS             7276..8451
                     /locus_tag="ACHBBW_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9152691.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8455..9096
                     /locus_tag="ACHBBW_000986"
     CDS             8455..9096
                     /locus_tag="ACHBBW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152692.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9093..10010
                     /locus_tag="ACHBBW_000987"
     CDS             9093..10010
                     /locus_tag="ACHBBW_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9152693.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10007..10678
                     /locus_tag="ACHBBW_000988"
     CDS             10007..10678
                     /locus_tag="ACHBBW_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152694.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11219..11707)
                     /locus_tag="ACHBBW_000989"
     CDS             complement(11219..11707)
                     /locus_tag="ACHBBW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9152695.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11908..12174)
                     /locus_tag="ACHBBW_000990"
     CDS             complement(11908..12174)
                     /locus_tag="ACHBBW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9152696.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12174..12437)
                     /locus_tag="ACHBBW_000991"
     CDS             complement(12174..12437)
                     /locus_tag="ACHBBW_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9152697.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12958..13098
                     /locus_tag="ACHBBW_000992"
                     /pseudo
     CDS             12958..13098
                     /locus_tag="ACHBBW_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13240..15063)
                     /locus_tag="ACHBBW_000993"
     CDS             complement(13240..15063)
                     /locus_tag="ACHBBW_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9152698.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15661..18366)
                     /locus_tag="ACHBBW_000994"
     CDS             complement(15661..18366)
                     /locus_tag="ACHBBW_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9152699.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18672..19619)
                     /locus_tag="ACHBBW_000995"
     CDS             complement(18672..19619)
                     /locus_tag="ACHBBW_000995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9152700.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19809..20078
                     /gene="rpsN"
                     /locus_tag="ACHBBW_000996"
     CDS             19809..20078
                     /gene="rpsN"
                     /locus_tag="ACHBBW_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9152701.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20254..20616)
                     /locus_tag="ACHBBW_000997"
     CDS             complement(20254..20616)
                     /locus_tag="ACHBBW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9152702.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20627..21748)
                     /gene="alr"
                     /locus_tag="ACHBBW_000998"
     CDS             complement(20627..21748)
                     /gene="alr"
                     /locus_tag="ACHBBW_000998"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9152703.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21764..22114)
                     /gene="acpS"
                     /locus_tag="ACHBBW_000999"
     CDS             complement(21764..22114)
                     /gene="acpS"
                     /locus_tag="ACHBBW_000999"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9152704.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22244..23755)
                     /locus_tag="ACHBBW_001000"
     CDS             complement(22244..23755)
                     /locus_tag="ACHBBW_001000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9152705.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24077..25429)
                     /gene="murF"
                     /locus_tag="ACHBBW_001001"
     CDS             complement(24077..25429)
                     /gene="murF"
                     /locus_tag="ACHBBW_001001"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9152706.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25582..26658)
                     /locus_tag="ACHBBW_001002"
     CDS             complement(25582..26658)
                     /locus_tag="ACHBBW_001002"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9152707.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26854..28599
                     /locus_tag="ACHBBW_001003"
     CDS             26854..28599
                     /locus_tag="ACHBBW_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9152708.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28879..29316)
                     /locus_tag="ACHBBW_001004"
     CDS             complement(28879..29316)
                     /locus_tag="ACHBBW_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9152709.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29300..30673)
                     /locus_tag="ACHBBW_001005"
     CDS             complement(29300..30673)
                     /locus_tag="ACHBBW_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152710.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(30995..31483)
                     /locus_tag="ACHBBW_001006"
     CDS             complement(30995..31483)
                     /locus_tag="ACHBBW_001006"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152711.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31505..32107)
                     /locus_tag="ACHBBW_001007"
     CDS             complement(31505..32107)
                     /locus_tag="ACHBBW_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9152712.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9187 a         6877 c         5938 g        10148 t
ORIGIN      
        1 cccatgctct tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct
       61 acctcgtctt taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag
      121 cgtttctgtt gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac
      181 agtagtcgtt gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg
      241 atcgtactca aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat
      301 ttctgaaaca actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct
      361 atctaacaag ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca
      421 cattgctaat agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc
      481 actaattctg tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt
      541 acttaatttt taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta
      601 ttcttcaaaa aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac
      661 ttgtttcatt agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa
      721 tgtttcatca catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata
      781 acaatcacat ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac
      841 tagacggcct tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca
      901 gtcaaacatg tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc
      961 catcaagtaa cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt
     1021 catttctgat ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat
     1081 cccgactaga tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat
     1141 tccaaataat ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg
     1201 agacgtacgt tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc
     1261 gtcaaaactc atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg
     1321 tgataaaaac atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat
     1381 attccgcata tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg
     1441 atcccagttc ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg
     1501 tagccataaa tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc
     1561 tggggacatt gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa
     1621 agttccgtgt ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat
     1681 taaacgatag cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct
     1741 tgacaactcc caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt
     1801 ctactttatt taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa
     1861 cattatgaca gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg
     1921 aaaaatcatg caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg
     1981 ttggggacaa gctgttttac taaatctaat ccctacattg attggtattg ctactattct
     2041 tcttattatg attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt
     2101 ctcagaagcg acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag
     2161 catcattagc gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg
     2221 acaaaaagat cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat
     2281 cggcggagtg atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct
     2341 attagttatt cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta
     2401 cgatatcgtc caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg
     2461 aaaattgatg gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg
     2521 gcatatctta gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc
     2581 tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact
     2641 tgtgaccgag ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca
     2701 gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc
     2761 cagtaaaaag aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa
     2821 tcgatggaac ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa
     2881 gaaccagtga ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa
     2941 agcaacagcc gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt
     3001 cgctaaattg actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga
     3061 tgaataagct aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca
     3121 tgggctgata gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa
     3181 gacccctgca tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt
     3241 cggaatgggt ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc
     3301 tggaagtcca tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc
     3361 ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa
     3421 aaagttgata gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac
     3481 gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt
     3541 tcctgtgatc cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac
     3601 accatcccca gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat
     3661 cttgtcttct ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc
     3721 ttcgtctgat aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat
     3781 acctaattct ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt
     3841 tttgatgcca gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc
     3901 gatcattccg atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc
     3961 ttgcggttct tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc
     4021 aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag
     4081 aacatcgaat gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt
     4141 catcattttt cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa
     4201 ttctgccttt gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt
     4261 acctttgata acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc
     4321 cattttcaat atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc
     4381 ttctttgctc catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc
     4441 tgagcagata acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc
     4501 atgtttcctc gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa
     4561 ggtttccggt acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg
     4621 ttctccttgc agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact
     4681 gattcgtttg cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc
     4741 gttcaaaaga tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc
     4801 tttcccacga cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa
     4861 tgactcatct tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt
     4921 caacgccgat tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat
     4981 agcatcaccg gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt
     5041 ggtcactttg ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc
     5101 cagtgctttt tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat
     5161 agcgatgatc agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa
     5221 ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa
     5281 ttttttgatc atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg
     5341 ccgttcatcc acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt
     5401 tacttcttct attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac
     5461 aaaaaaaggt acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt
     5521 atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa
     5581 aggatgctat tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct
     5641 cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt
     5701 gtagagtaca aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga
     5761 aacaagaagt aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg
     5821 aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa
     5881 gaaattcaac accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg
     5941 aatccttccg aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata
     6001 aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac
     6061 gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc
     6121 agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg
     6181 caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa
     6241 aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc
     6301 taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg
     6361 gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc
     6421 ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata
     6481 gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc
     6541 agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg
     6601 gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat
     6661 ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc
     6721 tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg
     6781 tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac
     6841 ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt
     6901 attattcata actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt
     6961 atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt
     7021 taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa
     7081 catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt
     7141 ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta
     7201 tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga
     7261 aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa
     7321 gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt
     7381 acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca
     7441 caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga
     7501 cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa
     7561 aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct
     7621 gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa
     7681 ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat
     7741 atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa
     7801 gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac
     7861 atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc
     7921 caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg
     7981 attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg
     8041 ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg
     8101 agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt
     8161 atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg
     8221 aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt
     8281 ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc
     8341 ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca
     8401 actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag
     8461 cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt
     8521 tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca
     8581 cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct
     8641 tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt
     8701 atcgcactct ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa
     8761 aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc
     8821 attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct
     8881 gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc
     8941 gatttgattt ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc
     9001 cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg
     9061 acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg
     9121 ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag
     9181 atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc
     9241 tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg
     9301 ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc
     9361 gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa
     9421 aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt
     9481 cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc
     9541 atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata
     9601 cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct
     9661 ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa
     9721 aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca
     9781 tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca
     9841 agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca
     9901 gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag
     9961 tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga agcaattgga
    10021 taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt
    10081 tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt
    10141 cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg
    10201 tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg
    10261 attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat
    10321 catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa
    10381 aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc
    10441 tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg
    10501 tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga
    10561 gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga
    10621 ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg
    10681 ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc
    10741 tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct
    10801 tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc
    10861 tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc
    10921 atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga
    10981 attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc
    11041 ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact
    11101 tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct
    11161 agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt
    11221 atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac
    11281 ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat
    11341 cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt
    11401 taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg
    11461 ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca
    11521 acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca
    11581 tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat
    11641 aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc
    11701 ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca
    11761 aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc
    11821 caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac
    11881 tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc
    11941 tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg
    12001 tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg
    12061 atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat
    12121 ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga
    12181 gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt
    12241 actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct
    12301 tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta
    12361 tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc
    12421 atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt
    12481 aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac
    12541 atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt
    12601 acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt
    12661 cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag
    12721 tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta
    12781 ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt
    12841 ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat
    12901 caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag
    12961 attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat
    13021 ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc
    13081 aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta
    13141 atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat
    13201 taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct
    13261 ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt
    13321 ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt
    13381 agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg
    13441 attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt
    13501 tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat
    13561 cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt
    13621 cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt
    13681 ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa
    13741 cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa
    13801 aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa
    13861 ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg
    13921 aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa
    13981 cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc
    14041 cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac
    14101 tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg
    14161 aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt
    14221 gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa
    14281 gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt
    14341 cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga
    14401 agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc
    14461 agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa
    14521 cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag
    14581 cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg
    14641 accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc
    14701 tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc
    14761 aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg
    14821 acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga
    14881 ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata
    14941 tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt
    15001 gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc
    15061 caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag
    15121 taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa
    15181 ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct
    15241 aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag
    15301 aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt
    15361 tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg
    15421 gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg
    15481 tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac
    15541 acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac
    15601 tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt
    15661 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
    15721 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
    15781 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
    15841 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
    15901 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
    15961 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
    16021 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
    16081 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
    16141 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
    16201 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
    16261 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
    16321 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
    16381 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
    16441 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
    16501 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
    16561 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
    16621 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
    16681 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
    16741 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
    16801 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
    16861 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
    16921 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
    16981 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
    17041 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
    17101 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
    17161 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
    17221 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
    17281 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
    17341 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
    17401 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
    17461 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
    17521 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
    17581 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
    17641 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
    17701 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
    17761 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
    17821 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
    17881 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
    17941 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
    18001 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
    18061 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
    18121 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
    18181 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
    18241 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
    18301 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
    18361 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
    18421 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
    18481 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
    18541 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
    18601 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
    18661 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
    18721 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
    18781 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
    18841 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
    18901 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
    18961 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
    19021 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
    19081 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
    19141 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
    19201 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
    19261 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
    19321 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
    19381 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
    19441 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
    19501 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
    19561 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
    19621 gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg
    19681 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
    19741 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
    19801 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
    19861 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
    19921 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
    19981 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
    20041 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
    20101 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
    20161 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
    20221 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
    20281 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
    20341 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
    20401 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
    20461 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
    20521 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
    20581 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
    20641 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
    20701 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
    20761 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
    20821 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
    20881 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
    20941 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
    21001 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
    21061 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
    21121 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
    21181 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
    21241 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
    21301 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
    21361 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
    21421 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
    21481 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
    21541 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
    21601 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
    21661 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
    21721 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
    21781 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
    21841 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
    21901 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
    21961 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
    22021 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
    22081 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
    22141 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
    22201 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
    22261 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
    22321 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
    22381 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
    22441 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
    22501 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
    22561 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
    22621 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
    22681 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
    22741 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
    22801 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
    22861 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
    22921 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
    22981 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
    23041 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
    23101 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
    23161 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
    23221 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
    23281 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca
    23341 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
    23401 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
    23461 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
    23521 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
    23581 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
    23641 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
    23701 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
    23761 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
    23821 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
    23881 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
    23941 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
    24001 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
    24061 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
    24121 tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat
    24181 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
    24241 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
    24301 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
    24361 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
    24421 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
    24481 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
    24541 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
    24601 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
    24661 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
    24721 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
    24781 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
    24841 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
    24901 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
    24961 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
    25021 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
    25081 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
    25141 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
    25201 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
    25261 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
    25321 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
    25381 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
    25441 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
    25501 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
    25561 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
    25621 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    25681 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    25741 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    25801 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    25861 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    25921 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    25981 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    26041 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    26101 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    26161 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    26221 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    26281 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    26341 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    26401 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    26461 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    26521 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    26581 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    26641 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    26701 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    26761 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    26821 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    26881 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    26941 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    27001 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    27061 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    27121 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    27181 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    27241 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    27301 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    27361 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    27421 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    27481 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    27541 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    27601 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    27661 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    27721 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    27781 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    27841 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    27901 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    27961 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    28021 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    28081 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    28141 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    28201 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    28261 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    28321 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    28381 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    28441 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    28501 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    28561 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    28621 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    28681 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    28741 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    28801 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    28861 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    28921 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    28981 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    29041 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    29101 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    29161 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    29221 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    29281 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    29341 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    29401 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    29461 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    29521 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    29581 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    29641 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    29701 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    29761 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    29821 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    29881 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    29941 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    30001 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    30061 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    30121 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    30181 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    30241 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    30301 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    30361 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    30421 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    30481 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    30541 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    30601 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    30661 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    30721 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    30781 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    30841 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    30901 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    30961 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    31021 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    31081 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    31141 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    31201 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    31261 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    31321 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    31381 cttgtttttg accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca
    31441 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    31501 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    31561 ccaccgacca cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    31621 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    31681 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    31741 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    31801 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    31861 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    31921 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    31981 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    32041 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    32101 tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAYYKC010000021        31179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000021 DAYYKC010000000
VERSION     DAYYKC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.021"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHBBW_001008"
     CDS             3..371
                     /locus_tag="ACHBBW_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9152713.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHBBW_001009"
     CDS             551..1492
                     /locus_tag="ACHBBW_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9152714.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHBBW_001010"
     CDS             1561..2292
                     /locus_tag="ACHBBW_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9152715.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHBBW_001011"
     CDS             2436..4616
                     /locus_tag="ACHBBW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9152716.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHBBW_001012"
     CDS             4636..5118
                     /locus_tag="ACHBBW_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9152717.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHBBW_001013"
     CDS             5134..5895
                     /locus_tag="ACHBBW_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9152718.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHBBW_001014"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHBBW_001014"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9152719.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHBBW_001015"
     CDS             7667..8152
                     /locus_tag="ACHBBW_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9152720.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHBBW_001016"
     CDS             8145..8828
                     /locus_tag="ACHBBW_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9152721.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHBBW_001017"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHBBW_001017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9152722.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHBBW_001018"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHBBW_001018"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9152723.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHBBW_001019"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHBBW_001019"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9152724.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHBBW_001020"
     CDS             13125..13457
                     /locus_tag="ACHBBW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9152725.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHBBW_001021"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHBBW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9152726.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHBBW_001022"
     CDS             14283..15656
                     /locus_tag="ACHBBW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9152727.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHBBW_001023"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHBBW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9152728.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHBBW_001024"
     CDS             16711..17151
                     /locus_tag="ACHBBW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9152729.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHBBW_001025"
     CDS             17295..17996
                     /locus_tag="ACHBBW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9152730.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHBBW_001026"
     CDS             17993..19114
                     /locus_tag="ACHBBW_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9152731.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHBBW_001027"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHBBW_001027"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9152732.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHBBW_001028"
     CDS             20493..21401
                     /locus_tag="ACHBBW_001028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9152733.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHBBW_001029"
     CDS             21436..21708
                     /locus_tag="ACHBBW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9152734.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHBBW_001030"
     CDS             21719..22765
                     /locus_tag="ACHBBW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9152735.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACHBBW_001031"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACHBBW_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9152736.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACHBBW_001032"
     CDS             complement(25776..26462)
                     /locus_tag="ACHBBW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152737.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACHBBW_001033"
     CDS             complement(26440..26934)
                     /locus_tag="ACHBBW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9152738.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACHBBW_001034"
     CDS             complement(26954..28174)
                     /locus_tag="ACHBBW_001034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152739.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACHBBW_001035"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACHBBW_001035"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9152740.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..>31179
                     /locus_tag="ACHBBW_001036"
     CDS             30352..>31179
                     /locus_tag="ACHBBW_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152741.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     "
BASE COUNT        10526 a         5480 c         6699 g         8474 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggt
//
LOCUS       DAYYKC010000022        30027 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000022 DAYYKC010000000
VERSION     DAYYKC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.022"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHBBW_001037"
     CDS             complement(61..2424)
                     /locus_tag="ACHBBW_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM9152742.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHBBW_001038"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHBBW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHBBW_001039"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHBBW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9152743.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHBBW_001040"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHBBW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9152744.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHBBW_001041"
     CDS             complement(5894..6205)
                     /locus_tag="ACHBBW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9152745.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHBBW_001042"
     CDS             complement(6202..6495)
                     /locus_tag="ACHBBW_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9152746.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHBBW_001043"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHBBW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9152747.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHBBW_001044"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHBBW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9152748.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHBBW_001045"
     CDS             complement(8333..12685)
                     /locus_tag="ACHBBW_001045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9152749.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHBBW_001046"
     CDS             complement(12897..14606)
                     /locus_tag="ACHBBW_001046"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9152750.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHBBW_001047"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHBBW_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9152751.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHBBW_001048"
     CDS             complement(16103..16903)
                     /locus_tag="ACHBBW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9152752.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHBBW_001049"
     CDS             complement(16900..17712)
                     /locus_tag="ACHBBW_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9152753.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHBBW_001050"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHBBW_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9152754.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHBBW_001051"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHBBW_001051"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9152755.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHBBW_001052"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHBBW_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9152756.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHBBW_001053"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHBBW_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9152757.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHBBW_001054"
     CDS             21446..21925
                     /locus_tag="ACHBBW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9152758.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGITATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHBBW_001055"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHBBW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHBBW_001056"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHBBW_001056"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9152759.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHBBW_001057"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHBBW_001057"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9152760.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHBBW_001058"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHBBW_001058"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9152761.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHBBW_001059"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHBBW_001059"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9152762.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHBBW_001060"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHBBW_001060"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9152763.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8013 a         6456 c         5343 g        10215 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcattacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattc
//
LOCUS       DAYYKC010000023        29444 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000023 DAYYKC010000000
VERSION     DAYYKC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.023"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..785
                     /locus_tag="ACHBBW_001061"
     CDS             129..785
                     /locus_tag="ACHBBW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9152764.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1227..2024)
                     /locus_tag="ACHBBW_001062"
     CDS             complement(1227..2024)
                     /locus_tag="ACHBBW_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9152765.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2021..3205)
                     /locus_tag="ACHBBW_001063"
     CDS             complement(2021..3205)
                     /locus_tag="ACHBBW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9152766.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3230..4477)
                     /locus_tag="ACHBBW_001064"
     CDS             complement(3230..4477)
                     /locus_tag="ACHBBW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9152767.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4692..6437
                     /gene="dnaX"
                     /locus_tag="ACHBBW_001065"
     CDS             4692..6437
                     /gene="dnaX"
                     /locus_tag="ACHBBW_001065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9152768.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6457..6771
                     /locus_tag="ACHBBW_001066"
     CDS             6457..6771
                     /locus_tag="ACHBBW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9152769.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6796..7392
                     /gene="recR"
                     /locus_tag="ACHBBW_001067"
     CDS             6796..7392
                     /gene="recR"
                     /locus_tag="ACHBBW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9152770.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7456..8100
                     /gene="tmk"
                     /locus_tag="ACHBBW_001068"
     CDS             7456..8100
                     /gene="tmk"
                     /locus_tag="ACHBBW_001068"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9152771.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8115..8444
                     /locus_tag="ACHBBW_001069"
     CDS             8115..8444
                     /locus_tag="ACHBBW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9152772.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8458..9396
                     /gene="holB"
                     /locus_tag="ACHBBW_001070"
     CDS             8458..9396
                     /gene="holB"
                     /locus_tag="ACHBBW_001070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9152773.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9432..10256
                     /locus_tag="ACHBBW_001071"
     CDS             9432..10256
                     /locus_tag="ACHBBW_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9152774.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10249..10596
                     /locus_tag="ACHBBW_001072"
     CDS             10249..10596
                     /locus_tag="ACHBBW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9152775.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10665..11537
                     /gene="rsmI"
                     /locus_tag="ACHBBW_001073"
     CDS             10665..11537
                     /gene="rsmI"
                     /locus_tag="ACHBBW_001073"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9152776.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11646..12767
                     /gene="dinB"
                     /locus_tag="ACHBBW_001074"
     CDS             11646..12767
                     /gene="dinB"
                     /locus_tag="ACHBBW_001074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9152777.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12821..13423)
                     /gene="nrdG"
                     /locus_tag="ACHBBW_001075"
     CDS             complement(12821..13423)
                     /gene="nrdG"
                     /locus_tag="ACHBBW_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9152778.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13738..15927)
                     /gene="nrdD"
                     /locus_tag="ACHBBW_001076"
     CDS             complement(13738..15927)
                     /gene="nrdD"
                     /locus_tag="ACHBBW_001076"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9152779.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16253..17020
                     /locus_tag="ACHBBW_001077"
     CDS             16253..17020
                     /locus_tag="ACHBBW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152780.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17010..19052
                     /locus_tag="ACHBBW_001078"
     CDS             17010..19052
                     /locus_tag="ACHBBW_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152781.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19333..19482
                     /locus_tag="ACHBBW_001079"
     CDS             19333..19482
                     /locus_tag="ACHBBW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9152782.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19493..21007
                     /gene="dltA"
                     /locus_tag="ACHBBW_001080"
     CDS             19493..21007
                     /gene="dltA"
                     /locus_tag="ACHBBW_001080"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9152783.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            21004..22209
                     /gene="dltB"
                     /locus_tag="ACHBBW_001081"
     CDS             21004..22209
                     /gene="dltB"
                     /locus_tag="ACHBBW_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9152784.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22254..22487
                     /gene="dltC"
                     /locus_tag="ACHBBW_001082"
     CDS             22254..22487
                     /gene="dltC"
                     /locus_tag="ACHBBW_001082"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9152785.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            22490..23758
                     /gene="dltD"
                     /locus_tag="ACHBBW_001083"
     CDS             22490..23758
                     /gene="dltD"
                     /locus_tag="ACHBBW_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9152786.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            23899..24981
                     /locus_tag="ACHBBW_001084"
     CDS             23899..24981
                     /locus_tag="ACHBBW_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9152787.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24981..25784
                     /locus_tag="ACHBBW_001085"
     CDS             24981..25784
                     /locus_tag="ACHBBW_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9152788.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            25854..26594
                     /locus_tag="ACHBBW_001086"
     CDS             25854..26594
                     /locus_tag="ACHBBW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9152789.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26605..27609
                     /locus_tag="ACHBBW_001087"
     CDS             26605..27609
                     /locus_tag="ACHBBW_001087"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9152790.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(27654..>29444)
                     /locus_tag="ACHBBW_001088"
     CDS             complement(27654..>29444)
                     /locus_tag="ACHBBW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152791.1"
                     /translation="KWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTK
                     DDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILY
                     QRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISF
                     IYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSED
                     ILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLL
                     YATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCL
                     IYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLE
                     QHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSF
                     CNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHA
                     IENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVA
                     DRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9307 a         5184 c         6062 g         8891 t
ORIGIN      
        1 aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag taaaatacct
       61 ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg
      121 gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta
      181 tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata
      241 tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc
      301 ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca
      361 tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc
      421 gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt
      481 attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa
      541 agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag
      601 aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca
      661 tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca
      721 ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac aatattatct
      781 tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
      841 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
      901 acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt
      961 caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga
     1021 gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc
     1081 aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct
     1141 tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt
     1201 ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg
     1261 ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg
     1321 attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata
     1381 ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca
     1441 gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct
     1501 actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt
     1561 cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca
     1621 ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg
     1681 ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc
     1741 atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta
     1801 tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct
     1861 ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg
     1921 aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca
     1981 acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag taacctccga
     2041 tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct
     2101 ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga
     2161 cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat
     2221 tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat
     2281 ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt
     2341 tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt
     2401 taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg
     2461 tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa
     2521 cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg
     2581 taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca
     2641 cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt
     2701 tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta
     2761 ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt
     2821 agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac
     2881 aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac
     2941 cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg
     3001 ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac
     3061 gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc
     3121 agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag
     3181 gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg
     3241 ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat
     3301 ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag
     3361 gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac
     3421 atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt
     3481 tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc
     3541 ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca
     3601 taccacgtgc aatggaacct tgcacgtagc cttcacgtac gctaggatca cctagcatcg
     3661 ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta
     3721 cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat
     3781 ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg
     3841 cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat
     3901 tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa
     3961 tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat
     4021 agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt
     4081 ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt
     4141 caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat
     4201 ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca
     4261 aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg
     4321 ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga
     4381 ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc
     4441 cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag
     4501 ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga
     4561 ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa
     4621 ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa
     4681 gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag
     4741 acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa
     4801 cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga
     4861 ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg
     4921 aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca
     4981 gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa
     5041 aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca
     5101 atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga
     5161 ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac
     5221 ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca
     5281 gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg
     5341 cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg
     5401 ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg
     5461 tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag
     5521 aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc
     5581 aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg
     5641 cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa
     5701 atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg
     5761 cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag
     5821 taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta
     5881 atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga
     5941 caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac
     6001 agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc
     6061 agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg
     6121 cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg
     6181 tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa
     6241 gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag
     6301 aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag
     6361 aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta
     6421 cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg
     6481 caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg
     6541 ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca
     6601 ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc
     6661 gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg
     6721 gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact
     6781 gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg
     6841 aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg
     6901 aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat
     6961 ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct
     7021 tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa
     7081 aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa
     7141 gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat
     7201 gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat
     7261 ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct
     7321 gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga
     7381 cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga
     7441 gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga
     7501 aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc
     7561 attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt
     7621 gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga
     7681 agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc
     7741 gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc
     7801 gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat
     7861 cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc
     7921 gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag
     7981 ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa
     8041 gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag
     8101 gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt
     8161 tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct
     8221 tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag
     8281 cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta
     8341 cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg
     8401 ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg
     8461 aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt
     8521 tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa
     8581 catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg
     8641 ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg
     8701 gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa
     8761 ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa
     8821 atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt
     8881 ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt
     8941 caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag
     9001 cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca
     9061 gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc
     9121 gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa
     9181 tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag
     9241 gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag
     9301 ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct
     9361 gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa
     9421 ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt
     9481 attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc
     9541 aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc
     9601 ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa
     9661 aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg
     9721 agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt
     9781 ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat
     9841 ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg
     9901 gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg
     9961 tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt
    10021 tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa
    10081 aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag
    10141 ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg
    10201 aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga
    10261 tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt
    10321 gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa
    10381 aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa
    10441 aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga
    10501 ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt
    10561 tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc
    10621 ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa
    10681 gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt
    10741 tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg
    10801 aggatacgcg taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa
    10861 gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag
    10921 gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg
    10981 agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag
    11041 ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct
    11101 tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag
    11161 ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg aatttcttag
    11221 aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat
    11281 accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg
    11341 aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag
    11401 agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg
    11461 ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt
    11521 accatgaatt agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca
    11581 gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg
    11641 tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata
    11701 ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac
    11761 atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag
    11821 caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg
    11881 agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca
    11941 aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg
    12001 aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc
    12061 gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt
    12121 acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag
    12181 ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg
    12241 tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat
    12301 atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc
    12361 gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg
    12421 gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc
    12481 gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag
    12541 tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag
    12601 aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct
    12661 tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga
    12721 cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag
    12781 agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt
    12841 tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg
    12901 attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt
    12961 ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga
    13021 gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc
    13081 agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat
    13141 ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca
    13201 agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat cgaaaatttt
    13261 gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt
    13321 tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc
    13381 tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat
    13441 ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca
    13501 aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa
    13561 gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa
    13621 taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag
    13681 gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca
    13741 tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc
    13801 ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg
    13861 atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc
    13921 acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc
    13981 aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta
    14041 gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa
    14101 tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt
    14161 gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact
    14221 ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc
    14281 attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc
    14341 ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac
    14401 agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc
    14461 gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc
    14521 tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa
    14581 tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc
    14641 taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa
    14701 tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt
    14761 cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta
    14821 attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa
    14881 gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg
    14941 aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg
    15001 tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat
    15061 atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc
    15121 ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc
    15181 tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga
    15241 gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt
    15301 gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata
    15361 aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg
    15421 tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg
    15481 tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt
    15541 gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt
    15601 ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg
    15661 ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc
    15721 ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc
    15781 gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac
    15841 atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac
    15901 agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta
    15961 tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg
    16021 ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa
    16081 acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat
    16141 aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga
    16201 tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa
    16261 gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc
    16321 cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag
    16381 tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg
    16441 cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt
    16501 tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc
    16561 aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa
    16621 gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga
    16681 tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc
    16741 acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt
    16801 gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca
    16861 tgatccatat acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt
    16921 ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca
    16981 ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt
    17041 ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct
    17101 attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata
    17161 cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt
    17221 tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc
    17281 agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa
    17341 ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt
    17401 caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc
    17461 catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt
    17521 caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag
    17581 atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac
    17641 ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca
    17701 gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg
    17761 tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta
    17821 aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt
    17881 tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga
    17941 caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc
    18001 cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg
    18061 aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata
    18121 gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc
    18181 aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata
    18241 ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta
    18301 ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg
    18361 acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag
    18421 caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa
    18481 tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt
    18541 atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc
    18601 aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt
    18661 cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg
    18721 ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag
    18781 aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca
    18841 gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca
    18901 ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt
    18961 tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga
    19021 gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata
    19081 gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca
    19141 acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat
    19201 ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg
    19261 taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga
    19321 ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc
    19381 atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat
    19441 aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat
    19501 caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta
    19561 tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta
    19621 ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt
    19681 tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga
    19741 agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt
    19801 catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga
    19861 atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac
    19921 agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat
    19981 cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc
    20041 tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta
    20101 tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt
    20161 tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc
    20221 atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga
    20281 catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg
    20341 attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc
    20401 aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt
    20461 aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac
    20521 gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa
    20581 gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg
    20641 tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt
    20701 acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat
    20761 caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt
    20821 tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga
    20881 acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct
    20941 gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc
    21001 tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc
    21061 attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat
    21121 caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag
    21181 ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt
    21241 tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt
    21301 ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt
    21361 tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat
    21421 ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca
    21481 atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct
    21541 gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt
    21601 ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc
    21661 attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt
    21721 atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg
    21781 atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa
    21841 ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga
    21901 ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc
    21961 ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc
    22021 gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag
    22081 aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg
    22141 acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt
    22201 tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag
    22261 aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg
    22321 atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg
    22381 agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga gaggattggt
    22441 gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa
    22501 actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac
    22561 ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat
    22621 gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata
    22681 tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct
    22741 ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc
    22801 gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt
    22861 ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac
    22921 gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc
    22981 tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt
    23041 gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca
    23101 gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt
    23161 gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga
    23221 aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt
    23281 cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca
    23341 gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt
    23401 ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa
    23461 atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa
    23521 atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac
    23581 caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca
    23641 acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga
    23701 tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag
    23761 aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata
    23821 aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat
    23881 agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct
    23941 gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa
    24001 ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct
    24061 ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca
    24121 aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc
    24181 atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt
    24241 ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag
    24301 gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt
    24361 cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta
    24421 ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat
    24481 ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta
    24541 ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta
    24601 gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta
    24661 ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg
    24721 aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct
    24781 ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc
    24841 attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat
    24901 ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa
    24961 aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag
    25021 cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa
    25081 tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca
    25141 tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg
    25201 cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat
    25261 tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag
    25321 tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga
    25381 gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag
    25441 attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca
    25501 tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg
    25561 atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta
    25621 cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt
    25681 tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac
    25741 aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat
    25801 tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat
    25861 taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt
    25921 tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat
    25981 tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg
    26041 atcatattgc tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc
    26101 cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga
    26161 cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc
    26221 cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg
    26281 tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt
    26341 tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa
    26401 atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta
    26461 acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa
    26521 atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag
    26581 tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga
    26641 atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg
    26701 ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa
    26761 atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc
    26821 ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg
    26881 gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg
    26941 ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt
    27001 tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc
    27061 gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca
    27121 aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa
    27181 aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta
    27241 gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat
    27301 cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc
    27361 aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca
    27421 gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct
    27481 gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc
    27541 agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta
    27601 ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca
    27661 ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc
    27721 tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt
    27781 tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca
    27841 ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt
    27901 cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc
    27961 aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag
    28021 gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc
    28081 acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt
    28141 tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc
    28201 tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca
    28261 taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg
    28321 tcggtattta agatgtgatc gattagattg tagctgataa taacgattag aatgataata
    28381 tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa
    28441 atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg
    28501 ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta
    28561 gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc
    28621 agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag
    28681 taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa
    28741 tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat
    28801 acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga
    28861 cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca
    28921 gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt
    28981 ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag
    29041 actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag
    29101 agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga
    29161 tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt
    29221 tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg
    29281 tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt
    29341 atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt
    29401 cctgttaaaa ataacaatga taatataatg attatgctcc actt
//
LOCUS       DAYYKC010000024        29016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000024 DAYYKC010000000
VERSION     DAYYKC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.024"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHBBW_001089"
     CDS             complement(<1..1031)
                     /locus_tag="ACHBBW_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9152792.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHBBW_001090"
     CDS             complement(1113..1802)
                     /locus_tag="ACHBBW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9152793.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBBW_001091"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBBW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9152794.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBBW_001092"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBBW_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9152795.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(2952..3079)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3158..3847)
                     /gene="rplA"
                     /locus_tag="ACHBBW_001093"
     CDS             complement(3158..3847)
                     /gene="rplA"
                     /locus_tag="ACHBBW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9152796.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3951..4373)
                     /gene="rplK"
                     /locus_tag="ACHBBW_001094"
     CDS             complement(3951..4373)
                     /gene="rplK"
                     /locus_tag="ACHBBW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9152797.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4716..5384
                     /gene="sdaAB"
                     /locus_tag="ACHBBW_001095"
     CDS             4716..5384
                     /gene="sdaAB"
                     /locus_tag="ACHBBW_001095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9152798.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5397..6269
                     /gene="sdaAA"
                     /locus_tag="ACHBBW_001096"
     CDS             5397..6269
                     /gene="sdaAA"
                     /locus_tag="ACHBBW_001096"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9152799.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6360..7460)
                     /locus_tag="ACHBBW_001097"
                     /pseudo
     CDS             complement(6360..7460)
                     /locus_tag="ACHBBW_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7552..8439)
                     /locus_tag="ACHBBW_001098"
     CDS             complement(7552..8439)
                     /locus_tag="ACHBBW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9152800.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8516..9061)
                     /gene="nusG"
                     /locus_tag="ACHBBW_001099"
     CDS             complement(8516..9061)
                     /gene="nusG"
                     /locus_tag="ACHBBW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9152801.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9162..9332)
                     /gene="secE"
                     /locus_tag="ACHBBW_001100"
     CDS             complement(9162..9332)
                     /gene="secE"
                     /locus_tag="ACHBBW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9152802.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9356..9508)
                     /gene="rpmG"
                     /locus_tag="ACHBBW_001101"
     CDS             complement(9356..9508)
                     /gene="rpmG"
                     /locus_tag="ACHBBW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9152803.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9670..10314
                     /gene="cbpA"
                     /locus_tag="ACHBBW_001102"
     CDS             9670..10314
                     /gene="cbpA"
                     /locus_tag="ACHBBW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9152804.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10370..10873)
                     /locus_tag="ACHBBW_001103"
     CDS             complement(10370..10873)
                     /locus_tag="ACHBBW_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9152805.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10886..10932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11470..12435
                     /locus_tag="ACHBBW_001104"
     CDS             11470..12435
                     /locus_tag="ACHBBW_001104"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9152806.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12534..13706)
                     /locus_tag="ACHBBW_001105"
     CDS             complement(12534..13706)
                     /locus_tag="ACHBBW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152807.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13693..14838)
                     /locus_tag="ACHBBW_001106"
     CDS             complement(13693..14838)
                     /locus_tag="ACHBBW_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152808.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14852..15784)
                     /locus_tag="ACHBBW_001107"
     CDS             complement(14852..15784)
                     /locus_tag="ACHBBW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152809.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15901..16980
                     /locus_tag="ACHBBW_001108"
     CDS             15901..16980
                     /locus_tag="ACHBBW_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9152810.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16977..17595
                     /locus_tag="ACHBBW_001109"
                     /pseudo
     CDS             16977..17595
                     /locus_tag="ACHBBW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17705..17932)
                     /locus_tag="ACHBBW_001110"
     CDS             complement(17705..17932)
                     /locus_tag="ACHBBW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9152811.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18035..20623)
                     /locus_tag="ACHBBW_001111"
     CDS             complement(18035..20623)
                     /locus_tag="ACHBBW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9152812.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20643..21302)
                     /locus_tag="ACHBBW_001112"
     CDS             complement(20643..21302)
                     /locus_tag="ACHBBW_001112"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9152813.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21351..21854)
                     /gene="trmL"
                     /locus_tag="ACHBBW_001113"
     CDS             complement(21351..21854)
                     /gene="trmL"
                     /locus_tag="ACHBBW_001113"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9152814.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22075..22941)
                     /locus_tag="ACHBBW_001114"
     CDS             complement(22075..22941)
                     /locus_tag="ACHBBW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9152815.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23050..23691
                     /locus_tag="ACHBBW_001115"
     CDS             23050..23691
                     /locus_tag="ACHBBW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9152816.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23753..25114)
                     /gene="mgtE"
                     /locus_tag="ACHBBW_001116"
     CDS             complement(23753..25114)
                     /gene="mgtE"
                     /locus_tag="ACHBBW_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9152817.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25132..26031)
                     /locus_tag="ACHBBW_001117"
     CDS             complement(25132..26031)
                     /locus_tag="ACHBBW_001117"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9152818.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26033..26830)
                     /locus_tag="ACHBBW_001118"
     CDS             complement(26033..26830)
                     /locus_tag="ACHBBW_001118"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9152819.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26808..27512)
                     /locus_tag="ACHBBW_001119"
     CDS             complement(26808..27512)
                     /locus_tag="ACHBBW_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9152820.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27643..28218
                     /locus_tag="ACHBBW_001120"
     CDS             27643..28218
                     /locus_tag="ACHBBW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9152821.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28336..29013
                     /locus_tag="ACHBBW_001121"
     CDS             28336..29013
                     /locus_tag="ACHBBW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9152822.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8545 a         6144 c         5219 g         9108 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttgttcatca tctcttcact
     2941 tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg
     3001 aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg
     3061 gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt
     3121 atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat
     3181 ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg
     3241 ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt
     3301 catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt
     3361 aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac
     3421 cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag
     3481 tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt
     3541 tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt
     3601 tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa
     3661 cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt
     3721 caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa
     3781 caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt
     3841 tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat
     3901 atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca
     3961 gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct
     4021 gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc
     4081 gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat
     4141 agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa
     4201 ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg
     4261 aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt
     4321 gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat
     4381 acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat
     4441 ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc
     4501 aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg
     4561 ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact
     4621 tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta
     4681 caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg
     4741 tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg
     4801 ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt
     4861 atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg
     4921 gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat
     4981 atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact
     5041 ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg
     5101 gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta
     5161 ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta
     5221 tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg
     5281 aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac
     5341 tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt
     5401 ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga
     5461 tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg
     5521 aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg
     5581 tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact
     5641 ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca
     5701 atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg
     5761 gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct
     5821 tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag
     5881 aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt
     5941 gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata
     6001 tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta
     6061 atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa
     6121 gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt
     6181 cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg
     6241 cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat
     6301 tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat
     6361 taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc
     6421 gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct
     6481 agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga
     6541 gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg
     6601 ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc atctagttga
     6661 cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc gattactgca
     6721 tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt
     6781 ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt
     6841 ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat
     6901 ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc
     6961 gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat
     7021 ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct
     7081 gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct
     7141 actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc
     7201 gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag
     7261 tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct
     7321 ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg
     7381 cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca
     7441 ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac atataaatca
     7501 tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt
     7561 ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga
     7621 ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc
     7681 atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag
     7741 ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta
     7801 atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg
     7861 cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg
     7921 tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat
     7981 ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca
     8041 agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg
     8101 gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt
     8161 tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat
     8221 ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt
     8281 tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt
     8341 tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc
     8401 agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata
     8461 gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat
     8521 cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga
     8581 tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag
     8641 caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc
     8701 gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg
     8761 gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa
     8821 cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat
     8881 gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga
     8941 cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta
     9001 ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca
     9061 ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt
     9121 tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca
     9181 aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt
     9241 tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg
     9301 atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt
     9361 tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac
     9421 gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac
     9481 aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt
     9541 atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat
     9601 tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga
     9661 ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca
     9721 gagactagta cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg
     9781 ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat
     9841 cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct
     9901 acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt
     9961 ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg
    10021 ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt
    10081 gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc
    10141 agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg
    10201 atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag
    10261 aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa
    10321 agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct
    10381 tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga
    10441 taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa
    10501 ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac
    10561 gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg
    10621 tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga
    10681 tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac
    10741 tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg
    10801 ctagataaag agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt
    10861 tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg
    10921 aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt
    10981 cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg
    11041 gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag
    11101 gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg
    11161 agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga
    11221 accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt
    11281 tttcggtctt cggacctttt tcattttcct agacttctgc tataataacc aagaattaat
    11341 tttgtaacag atttttttga aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg
    11401 ctaattattg gttaaccagc aaacaggagt attaaaatat cttgttttat tgaatagcag
    11461 gaggaaatca tgaaattcca ttatcatata ttgatcaatc catcggcagg cagcggaaat
    11521 ggctataaag tagcagaaag aattttaccg gtattaaaaa ataagcatat tgactataca
    11581 cttcattata gtgaatacaa aggacacgat gctgagattg ctgaaacttt ggcaaaagaa
    11641 acattgattc cttggataga agaagaacag aaaacaatcg atactttccc ccttttgatc
    11701 atcgtaggag gtgacggcac cttacatcag gtacttgata ctttctatca gatggaagta
    11761 gaattcccag ttgcttatat tccagcaggt tctggaaatg attttgctcg aggggcaggc
    11821 cttccaaaaa atcccaaaaa aggacttcag ctgattttag cagctcaatc accagaaaaa
    11881 gtccacataa tggcttatga agaaaaaata agtgaaaaaa aaggaatagc tgttaataat
    11941 ttcggtatag gattagatgc agctatcgtc catgcgacaa atcattcttc cacaaaaaag
    12001 cgattgaaca aatacaactt aggatcattt tcgtatttat tctctattct ccgtgcttta
    12061 ttcactcaaa aaggatttcc aattctggta gatgcaggag gaaaacaatt aagtttttca
    12121 aacgcatttt tatgtacagc aacgaagcat cctttttttg gcggaggaat cgcgattgtt
    12181 ccaactgctg atgcgtctaa accagtcatt gattttgtcg tggtcgaacg aatcaatttc
    12241 tttaaaatct tgtggctcat tcttttactt atacagaaaa aacaattgaa atcgaaatat
    12301 ttccatcact acacaacaaa cagattacgt atcgtttcaa caacaccgca acatggtcaa
    12361 gaagatggag aagaaatgga aaaacgacct tttgatatca caataaccac aactaaacag
    12421 ttgttttggt gttaataaaa gaggttttgt cagaagctac atctggaaca ccgtttattc
    12481 ggaaaaaagg actgtgacac gacttttgtc acagtccttt ttttactatg cagttaattc
    12541 tttgcccaat tgggttgcca tcgatagttt ctttctttac ccaaaataaa ttggatcatt
    12601 gaaaacaccg cagcaaaagc aactaagaca caaccattga accaaaaaga ttctgcaaat
    12661 gtttgattgt aactgagtgt ttcactgatc aagttattat tttgaacaaa ccaatatgtt
    12721 ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt attctgctgg tacaaacacc
    12781 ccaccgataa agcaactgcc tgtgataaaa atatctttga tcccaccgct ggtttctgag
    12841 ttttttacca atgaagtgat acaagccgaa aagctaatga tcggcaataa aaacaagaaa
    12901 gtatttaaaa tatgatacca taccgttata tcccacgagg tgtggatcac taacagaacc
    12961 attagaagta agaagccact gaacaacagc agcgagtaac taatcaaact aaaggttact
    13021 ttgcgattca agtttcgttt ggtaatgggc gcactctggt ttcgtttact gatctctttg
    13081 cgattaaacg ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa caacccataa
    13141 gacaagaaat taaaggttgt gcccgtcaac agctttttca tccgctgact atatcccgca
    13201 cttagctcaa cttctccagt aaccgagagt gccatttgtg tctgttccat agctgcatcg
    13261 attgttttat cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt
    13321 gtaacaactg ttttagaaaa ggcatctggg cgactctgta tcttgatcgt cgccatcttt
    13381 cctacttcga cttgttcggt aaagtctgca ggcagatgta agataatgtt tacacgatta
    13441 aaataaagag catcatcgat ttctttttgt gaggttccat ccaatgtaac gactgtttcc
    13501 ttgtcattca aatatttacg aaggccatcg actacaggcg actcttcttc actaatcaag
    13561 gcgattttcg catcttgcag agaggtattt tcaacatcaa tattttgcgc aaaaaagaat
    13621 gtggcaacaa cagtgataat tacacctatc agtaaattcg atttatttgc tttaagcaat
    13681 gtccaaatcg ttttataaag cgtcataacg cacccctctt tccatccaag catttcctaa
    13741 cgcaaaaagg aaaataaatc cgatcaacca tagaagattg gtataaaaac tagccaaatc
    13801 agtgtagaaa taaagtttat acaaactttc gctgatcaag ttgacgagat ttaattgacc
    13861 aacaatcgga atatgaagat caatccaata cttcacttgt tccgacccca tcataccagc
    13921 aaaaaagctc atcgcaatcg tcactgaaat caagatgcta tccttttgca ccagattcat
    13981 ttttggtatc aaattaccaa ccaatgtacc taacaaaatc gcacacaacg accccagagc
    14041 ggtaacaatc atcaaccatt gccaacgttc accaaagtct acttgataga taaaacgaaa
    14101 gacagctaac ataatataaa tctctgtcaa aacaatcgta aagcttgctg ccaaattagc
    14161 gattgaaact aacgattgct ttctaggaat caaggacaag cgaataccat ttgacgattg
    14221 atttgcttgc tgatcctgta cattgcggac cccccacatc gtcccgtaga gaatcgccat
    14281 tccaatcaga gtgaagaaat aaaaactttt gaaactcata ttacgacctg catgaacttc
    14341 ttgaaaagat acttcttgtt caaaaacagt catgatgggc gcgagatcaa cgttctccac
    14401 ttctgccact ttactcgaaa ttataccaat acgctgtaaa aattgatcca tgacgttttt
    14461 catcaacgtc tgactcattc ctgcttgacc cacatggagc gaaacagatg aaccatcaat
    14521 gagataaaaa ccagccaaat catctgcatt tagcttttca ttcgcttcta cttccgatag
    14581 ttcctgtact tcaaataatg gtttatcatt gacttcgact tgttctaaga ctgtggtaaa
    14641 attttccgaa agcgctgttt gatccgcttt gattacaatt ccagtattaa tcgtttcaag
    14701 attgccacct tgatcaatct cgccaaaggc cgccataaac atcaatccta aaagaattgg
    14761 aaaggccaat gtccaaaaca acatggattt atttcgcagc agtatttttg tgcgatattt
    14821 ataaaaatgc caaaacatta tttcccctcc tctagtctct caaatccttg ccagtaatct
    14881 ctaaaaagac atcattcaag gacgcttctt tggttgctat ttttccgtaa ctgatctttt
    14941 cttcttgtaa gattgtaaga atcggcaaca atactgtatt tagttcagta gttgttactt
    15001 ggagctgacc ttttttataa gcgattccat cagaagctat tgttcgctga atcgctgaca
    15061 acacattttc tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat
    15121 cttttagaga ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct
    15181 ggtcgcaaag aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt
    15241 gtcgattcag ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta
    15301 ctgtcggctc atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca
    15361 accgacgttt caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc
    15421 ccactaattg aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata
    15481 aaccgcaaaa ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt
    15541 gaggaacgac cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga
    15601 acaatttaat ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg
    15661 atttcccact gccgtttgga cctaacagac ctaagacttc tccttcttgt acttgcagat
    15721 caaaatgatt caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa
    15781 tcatcatgaa aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa
    15841 agaaactagt gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct
    15901 atgcataaat ctcggctact caacgaaact agctttctac tggttatctc attgcttttg
    15961 ctggtaagtc aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc
    16021 actggtgtca gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt
    16081 cttttatgtg cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc
    16141 gcccctgaag aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg
    16201 gttggtaatc atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt
    16261 tacttacgaa aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa
    16321 gatactcatt gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca
    16381 caagaaacac aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc
    16441 catgacaatg tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg
    16501 atcaatcaag aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact
    16561 gggatggatc ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa
    16621 cactccttag agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg
    16681 tttgaaggat tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta
    16741 accaacagta tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg
    16801 gccttttatc gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc
    16861 ggtattggtt tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt
    16921 ttccaagaaa accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga
    16981 taacagtagt cattattgat gatgatccat ttgtgacgaa atcgttacaa accattttag
    17041 aaagcacgaa agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgcgaaacc
    17101 catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg catcgatgct
    17161 gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac gacattcaaa
    17221 gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt aatcaaacag
    17281 aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc cgtatttggt
    17341 aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat tgatagtcat
    17401 gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc tgccgggaaa
    17461 aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg caattacatc
    17521 agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat ctatttttat
    17581 cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc cttcgcatca
    17641 ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct cacgattgaa
    17701 agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg ttaggcggaa
    17761 aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg tctcgttcta
    17821 catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt ttaaagtcat
    17881 tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc ataaagtaac
    17941 gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa aacgctaacc
    18001 aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc ggtataaaat
    18061 cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta tcgatcactt
    18121 ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc ttttcttctc
    18181 caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt ttcgtttcat
    18241 tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc tcttttgtat
    18301 agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct gctagtttgg
    18361 tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac gtagtcatcc
    18421 ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct aacaaaatca
    18481 acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc atccgctgat
    18541 attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat ttatggattg
    18601 agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg acctcatttg
    18661 catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc aagatgatac
    18721 ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg actaattgga
    18781 ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt tttccaggtt
    18841 tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct gcaggtcccc
    18901 gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt ttcgcttttt
    18961 ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa taggaacgat
    19021 ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca tgggcaagag
    19081 gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt ttgggaattt
    19141 gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga agctgatctt
    19201 tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat gtgttagcca
    19261 gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct aattctttcg
    19321 tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg atcgtactgg
    19381 caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt gctgcaagta
    19441 gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg ttcaactctg
    19501 caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt cctcctgtta
    19561 aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa ggtccatact
    19621 gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt tcaggataat
    19681 ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc cattcagaaa
    19741 agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct tcttcaacaa
    19801 gttggataat tacagtagca acctgatcag gctggatttc aattgggcga cttgtttcta
    19861 aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct cctgtttgaa
    19921 ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt gaatctgctg
    19981 caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct tctatgtctt
    20041 cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta taggcctggt
    20101 agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg atcacttgat
    20161 ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc ttcgtcaaac
    20221 cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca tcttctccca
    20281 gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt tcgctggaaa
    20341 gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt tcgagtttaa
    20401 gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa gtctcatttt
    20461 cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa tcagctgtgg
    20521 tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga aaaaaaatcg
    20581 cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc cgcctccttc
    20641 ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg gttccatact
    20701 ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt caaactacta
    20761 tttaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat ccattgaatg
    20821 gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt atgtcgggca
    20881 accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt ttctccgtga
    20941 aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt atccggatat
    21001 ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag ttctcttagc
    21061 ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc ttctgctgta
    21121 tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata ttttaccttt
    21181 ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc gccattgctc
    21241 ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa atatagttcc
    21301 atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt ctaatccagc
    21361 ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg gcgaagtgct
    21421 tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg ctcatcattc
    21481 atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg caaaccggtt
    21541 gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc gtcactatat
    21601 actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa tgttaaaaaa
    21661 gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa ccctgcccgt
    21721 tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag cggagtattc
    21781 gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg ttcaaataaa
    21841 acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat tttctgacaa
    21901 tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg agtcgttacg
    21961 ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa accaatgaaa
    22021 aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta ctcattaaaa
    22081 actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa ttgtaatctt
    22141 cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc attctaaagg
    22201 tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat aggttacacg
    22261 ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt cattggaata
    22321 actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta aataattttc
    22381 ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga attcttggta
    22441 atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa acggcaaatt
    22501 ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg cgtagactcc
    22561 ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg aaagttcatt
    22621 caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag gtaaatgctg
    22681 cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac gccttggaac
    22741 gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa gtgttccttt
    22801 tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag acttttttac
    22861 ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa actggcgtcc
    22921 tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac cgttagatgc
    22981 tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa agataaagga
    23041 gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc tctagcaatt
    23101 gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg cacaaccccg
    23161 agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat ccccgtaatg
    23221 acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt gaaaatcatg
    23281 catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt tggttgtctt
    23341 actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac agcagaaggg
    23401 ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca attcgaagca
    23461 atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg tcctgctgac
    23521 aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac agatggccgc
    23581 ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt agctaaagaa
    23641 ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata atcttataaa
    23701 caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt ttttacataa
    23761 aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc aaatcgctca
    23821 aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga tccattagca
    23881 tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata gccatcccta
    23941 tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa atcgtgatcc
    24001 ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt cgaacaaatc
    24061 catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc gactgggttc
    24121 cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata ctcgcttcac
    24181 ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa aacaacagag
    24241 tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta atttcttcca
    24301 cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata acgtcgatga
    24361 tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca ggaagagcta
    24421 agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct acatgaactg
    24481 aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc acgattaggt
    24541 cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag atgtaataga
    24601 ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga acagtttgat
    24661 tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca tcctcgtaat
    24721 ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa tatttagcaa
    24781 tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac atctcagaaa
    24841 gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata tgttcgttgt
    24901 cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag gaatagatca
    24961 gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag atatgcatct
    25021 ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga gtcaactggg
    25081 caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc cttattcttc
    25141 aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact ccagcaactc
    25201 ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc tttttattcc
    25261 cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa ttgcggaaaa
    25321 atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta acgcgatttc
    25381 tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat cagagaccat
    25441 tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc catgtgccga
    25501 taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact tcttttcacg
    25561 aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca tcaagccaga
    25621 tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac cttgtttttt
    25681 ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg caggaataga
    25741 cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca cgatatcgat
    25801 cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca caatggttac
    25861 cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa ctttgatttg
    25921 accgccttga aattttattt tcgctaacag accctttgag atcccttttt cttttaaaaa
    25981 atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca tatcaatcct
    26041 cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa gaagcaaaat
    26101 ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt gcgacattca
    26161 attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct agccattcat
    26221 catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc agtgaagcaa
    26281 tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg acacttttat
    26341 tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta aaactttcaa
    26401 acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt ttaatcgttg
    26461 attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg atccgaacat
    26521 ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct actagttctt
    26581 ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc acgcccaaga
    26641 aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat gtgccgtctc
    26701 cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct gcttgttcta
    26761 aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta tgaacgatcg
    26821 ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt tcgatagtag
    26881 acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg gatttcttca
    26941 cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg ctgaagtcgt
    27001 tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg ctcaatcgtt
    27061 gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt cttctcaccg
    27121 ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc acttgctttt
    27181 ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt acggatgatc
    27241 tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc ggcaatatca
    27301 gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt tgttacaata
    27361 ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg attttgttca
    27421 cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt ttgttcatat
    27481 ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc attaacattc
    27541 attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg cattgaccaa
    27601 aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca aatgcttgaa
    27661 atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc tcactatcaa
    27721 ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt tgatgaacag
    27781 ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc tgaatataca
    27841 ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat aacagaagaa
    27901 gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt tcaaaaactt
    27961 tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac aacaaaacga
    28021 gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg agacaatcta
    28081 cacgattttg aactggaact agaggtaggg gatgcatctg tgggaaaaat tgcttttata
    28141 aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat ccaacgtatg
    28201 cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc atttttttac
    28261 ttttttctgt taagttatag gatgtgaaag aatctagtca ctagttgtat agaatctatt
    28321 ttgggggaga ttcatatgat cgaaatctat ttatttgtta atcctttagg ggaatactgt
    28381 ttcgaaatgg aacaacagtt attacaattt atcgaagaag agtacgggac accatcaaaa
    28441 gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc agacaattgg ggatgtcatg
    28501 caacaaaaag gaatttctca gaacaatctt gaggaacgta atcacttatt ctcaatgact
    28561 tattctgcag cattggactg caaagcggcg cagtttcaag gaaagaaaaa aggccgccgt
    28621 tttttgatca aattgcaaga agcagtcggc tgtaatgaca taccatattc tcaagaactt
    28681 gctgaatcca tatttgaaga aatcggcggc gatttgaata tgtttaaaga agatcgtcag
    28741 tctgacttcg ttaaagaagt attcttttct gatcaaaaaa ttgctcggga aatgggcatc
    28801 acaaaacacc cttctgcagt cgtttacaat tatgcctgcg accgtgattt tggtgtttta
    28861 gtagaagtaa aaggtacaag tagcctttgg agaatcacag agctgtgtaa aacaacagat
    28921 aactcatatc aagtttttaa tggtgaacat ttacatacaa atgattctca gttccattca
    28981 agaactagcc atttacactt gctttctaat taaaaa
//
LOCUS       DAYYKC010000025        27711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000025 DAYYKC010000000
VERSION     DAYYKC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.025"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1214)
                     /locus_tag="ACHBBW_001122"
     CDS             complement(270..1214)
                     /locus_tag="ACHBBW_001122"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9152823.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1510..2454)
                     /locus_tag="ACHBBW_001123"
     CDS             complement(1510..2454)
                     /locus_tag="ACHBBW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9152824.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2675..3889
                     /locus_tag="ACHBBW_001124"
     CDS             2675..3889
                     /locus_tag="ACHBBW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9152825.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3911..5926
                     /locus_tag="ACHBBW_001125"
     CDS             3911..5926
                     /locus_tag="ACHBBW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9152826.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6009..7382)
                     /locus_tag="ACHBBW_001126"
     CDS             complement(6009..7382)
                     /locus_tag="ACHBBW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9152827.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7408..7692)
                     /locus_tag="ACHBBW_001127"
     CDS             complement(7408..7692)
                     /locus_tag="ACHBBW_001127"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9152828.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7694..9709)
                     /locus_tag="ACHBBW_001128"
     CDS             complement(7694..9709)
                     /locus_tag="ACHBBW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9152829.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9888..10193)
                     /locus_tag="ACHBBW_001129"
     CDS             complement(9888..10193)
                     /locus_tag="ACHBBW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9152830.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10282..11313)
                     /locus_tag="ACHBBW_001130"
     CDS             complement(10282..11313)
                     /locus_tag="ACHBBW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9152831.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11520..12395
                     /locus_tag="ACHBBW_001131"
     CDS             11520..12395
                     /locus_tag="ACHBBW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9152832.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12547..13389)
                     /locus_tag="ACHBBW_001132"
     CDS             complement(12547..13389)
                     /locus_tag="ACHBBW_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9152833.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13481..15358)
                     /locus_tag="ACHBBW_001133"
     CDS             complement(13481..15358)
                     /locus_tag="ACHBBW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9152834.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15355..16776)
                     /locus_tag="ACHBBW_001134"
     CDS             complement(15355..16776)
                     /locus_tag="ACHBBW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9152835.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16779..20168)
                     /locus_tag="ACHBBW_001135"
     CDS             complement(16779..20168)
                     /locus_tag="ACHBBW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9152836.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20425..21816
                     /locus_tag="ACHBBW_001136"
     CDS             20425..21816
                     /locus_tag="ACHBBW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152837.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21889..22536)
                     /locus_tag="ACHBBW_001137"
     CDS             complement(21889..22536)
                     /locus_tag="ACHBBW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152838.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22544..23230)
                     /locus_tag="ACHBBW_001138"
     CDS             complement(22544..23230)
                     /locus_tag="ACHBBW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152839.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23236..24309)
                     /locus_tag="ACHBBW_001139"
     CDS             complement(23236..24309)
                     /locus_tag="ACHBBW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152840.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24531..25205
                     /locus_tag="ACHBBW_001140"
     CDS             24531..25205
                     /locus_tag="ACHBBW_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9152841.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEIIQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25210..26550
                     /locus_tag="ACHBBW_001141"
     CDS             25210..26550
                     /locus_tag="ACHBBW_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9152842.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26699..27685)
                     /locus_tag="ACHBBW_001142"
     CDS             complement(26699..27685)
                     /locus_tag="ACHBBW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9152843.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8077 a         5243 c         5032 g         9359 t
ORIGIN      
        1 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
       61 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
      121 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tatttatttg
      181 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
      241 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
      301 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
      361 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
      421 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
      481 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
      541 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
      601 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
      661 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
      721 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
      781 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
      841 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
      901 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
      961 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
     1021 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
     1081 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
     1141 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
     1201 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
     1261 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
     1321 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
     1381 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
     1441 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
     1501 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
     1561 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
     1621 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
     1681 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
     1741 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
     1801 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
     1861 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
     1921 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
     1981 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
     2041 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
     2101 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
     2161 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
     2221 attaataatt tttaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
     2281 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
     2341 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
     2401 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
     2461 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
     2521 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
     2581 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
     2641 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
     2701 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
     2761 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
     2821 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
     2881 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
     2941 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
     3001 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
     3061 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
     3121 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
     3181 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
     3241 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
     3301 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
     3361 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
     3421 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
     3481 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
     3541 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
     3601 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
     3661 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
     3721 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
     3781 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
     3841 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
     3901 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
     3961 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
     4021 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
     4081 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
     4141 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
     4201 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
     4261 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
     4321 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
     4381 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
     4441 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
     4501 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
     4561 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
     4621 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
     4681 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
     4741 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
     4801 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
     4861 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
     4921 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
     4981 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
     5041 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
     5101 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
     5161 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
     5221 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
     5281 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
     5341 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
     5401 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
     5461 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
     5521 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
     5581 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
     5641 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
     5701 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
     5761 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
     5821 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
     5881 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
     5941 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
     6001 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
     6061 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
     6121 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
     6181 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
     6241 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
     6301 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
     6361 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
     6421 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
     6481 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
     6541 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
     6601 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
     6661 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
     6721 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
     6781 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
     6841 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
     6901 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
     6961 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
     7021 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
     7081 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
     7141 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
     7201 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
     7261 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
     7321 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
     7381 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
     7441 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
     7501 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
     7561 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
     7621 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
     7681 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
     7741 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
     7801 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
     7861 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
     7921 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
     7981 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
     8041 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
     8101 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
     8161 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
     8221 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
     8281 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
     8341 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
     8401 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
     8461 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
     8521 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
     8581 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
     8641 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
     8701 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
     8761 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
     8821 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
     8881 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
     8941 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
     9001 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
     9061 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
     9121 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
     9181 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
     9241 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
     9301 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
     9361 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
     9421 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
     9481 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
     9541 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
     9601 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
     9661 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
     9721 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
     9781 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
     9841 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
     9901 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
     9961 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    10021 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    10081 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    10141 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    10201 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    10261 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    10321 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    10381 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    10441 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    10501 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    10561 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    10621 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    10681 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    10741 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    10801 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    10861 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    10921 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    10981 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    11041 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    11101 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    11161 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    11221 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    11281 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    11341 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    11401 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    11461 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    11521 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    11581 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    11641 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    11701 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    11761 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    11821 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    11881 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    11941 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    12001 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    12061 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    12121 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    12181 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    12241 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    12301 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    12361 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    12421 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    12481 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    12541 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    12601 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    12661 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    12721 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    12781 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    12841 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    12901 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    12961 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    13021 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    13081 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    13141 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    13201 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    13261 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    13321 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    13381 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    13441 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    13501 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    13561 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    13621 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    13681 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    13741 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    13801 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    13861 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    13921 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    13981 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    14041 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    14101 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    14161 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    14221 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    14281 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    14341 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    14401 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    14461 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    14521 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    14581 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    14641 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    14701 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    14761 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    14821 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    14881 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    14941 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    15001 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    15061 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    15121 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    15181 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    15241 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    15301 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    15361 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    15421 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    15481 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    15541 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    15601 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    15661 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    15721 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    15781 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    15841 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    15901 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    15961 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    16021 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    16081 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    16141 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    16201 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    16261 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    16321 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    16381 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    16441 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    16501 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    16561 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    16621 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    16681 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    16741 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    16801 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    16861 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    16921 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    16981 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    17041 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    17101 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    17161 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    17221 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    17281 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    17341 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    17401 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    17461 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    17521 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    17581 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    17641 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    17701 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    17761 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    17821 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    17881 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    17941 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    18001 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    18061 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    18121 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    18181 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    18241 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    18301 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    18361 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    18421 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    18481 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    18541 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    18601 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    18661 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    18721 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    18781 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    18841 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    18901 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    18961 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    19021 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    19081 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    19141 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    19201 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    19261 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    19321 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    19381 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    19441 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    19501 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    19561 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    19621 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    19681 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    19741 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    19801 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    19861 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    19921 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    19981 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    20041 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    20101 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    20161 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    20221 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    20281 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    20341 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    20401 ttgttggaag gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    20461 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    20521 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    20581 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    20641 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    20701 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    20761 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    20821 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    20881 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    20941 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    21001 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    21061 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    21121 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    21181 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    21241 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
    21301 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    21361 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    21421 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    21481 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    21541 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    21601 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    21661 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    21721 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    21781 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    21841 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    21901 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    21961 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    22021 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    22081 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    22141 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    22201 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    22261 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    22321 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    22381 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    22441 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    22501 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    22561 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    22621 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    22681 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    22741 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    22801 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    22861 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    22921 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    22981 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    23041 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    23101 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    23161 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    23221 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    23281 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    23341 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    23401 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    23461 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    23521 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    23581 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    23641 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
    23701 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
    23761 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
    23821 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
    23881 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
    23941 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
    24001 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
    24061 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
    24121 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
    24181 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
    24241 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
    24301 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
    24361 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
    24421 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
    24481 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
    24541 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
    24601 tcggcgagat aatacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
    24661 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
    24721 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
    24781 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
    24841 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
    24901 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
    24961 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
    25021 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
    25081 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
    25141 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
    25201 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
    25261 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
    25321 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
    25381 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
    25441 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
    25501 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
    25561 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
    25621 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
    25681 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
    25741 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
    25801 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
    25861 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
    25921 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
    25981 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
    26041 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
    26101 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
    26161 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
    26221 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
    26281 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
    26341 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
    26401 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
    26461 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
    26521 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
    26581 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
    26641 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
    26701 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
    26761 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
    26821 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
    26881 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
    26941 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
    27001 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
    27061 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
    27121 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
    27181 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
    27241 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
    27301 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
    27361 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
    27421 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
    27481 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
    27541 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
    27601 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
    27661 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt t
//
LOCUS       DAYYKC010000026        27620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000026 DAYYKC010000000
VERSION     DAYYKC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.026"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..639)
                     /locus_tag="ACHBBW_001143"
     CDS             complement(277..639)
                     /locus_tag="ACHBBW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152844.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="ACHBBW_001144"
     CDS             complement(713..1093)
                     /locus_tag="ACHBBW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9152845.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="ACHBBW_001145"
     CDS             1175..2350
                     /locus_tag="ACHBBW_001145"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9152846.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="ACHBBW_001146"
     CDS             complement(2427..3011)
                     /locus_tag="ACHBBW_001146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9152847.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="ACHBBW_001147"
     CDS             3212..4210
                     /locus_tag="ACHBBW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9152848.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="ACHBBW_001148"
     CDS             4322..4831
                     /locus_tag="ACHBBW_001148"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9152849.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="ACHBBW_001149"
     CDS             complement(4861..5520)
                     /locus_tag="ACHBBW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9152850.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="ACHBBW_001150"
     CDS             complement(5522..6286)
                     /locus_tag="ACHBBW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9152851.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="ACHBBW_001151"
     CDS             complement(6294..6971)
                     /locus_tag="ACHBBW_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9152852.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="ACHBBW_001152"
     CDS             complement(7008..8393)
                     /locus_tag="ACHBBW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9152853.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8362..8616)
                     /locus_tag="ACHBBW_001153"
     CDS             complement(8362..8616)
                     /locus_tag="ACHBBW_001153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152854.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="ACHBBW_001154"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBBW_001154"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHBBW_001155"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHBBW_001155"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9152855.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9331..10188
                     /locus_tag="ACHBBW_001156"
     CDS             9331..10188
                     /locus_tag="ACHBBW_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152856.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10269..10604)
                     /locus_tag="ACHBBW_001157"
     CDS             complement(10269..10604)
                     /locus_tag="ACHBBW_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152857.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10671..12041)
                     /locus_tag="ACHBBW_001158"
     CDS             complement(10671..12041)
                     /locus_tag="ACHBBW_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9152858.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12119..12655)
                     /locus_tag="ACHBBW_001159"
     CDS             complement(12119..12655)
                     /locus_tag="ACHBBW_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9152859.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12964..14445
                     /locus_tag="ACHBBW_001160"
     CDS             12964..14445
                     /locus_tag="ACHBBW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152860.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14548..15255)
                     /locus_tag="ACHBBW_001161"
     CDS             complement(14548..15255)
                     /locus_tag="ACHBBW_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9152861.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15334..15672)
                     /locus_tag="ACHBBW_001162"
     CDS             complement(15334..15672)
                     /locus_tag="ACHBBW_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9152862.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15783..16745)
                     /locus_tag="ACHBBW_001163"
     CDS             complement(15783..16745)
                     /locus_tag="ACHBBW_001163"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9152863.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16760..18091)
                     /locus_tag="ACHBBW_001164"
     CDS             complement(16760..18091)
                     /locus_tag="ACHBBW_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9152864.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18099..18779)
                     /locus_tag="ACHBBW_001165"
     CDS             complement(18099..18779)
                     /locus_tag="ACHBBW_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9152865.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18998..19477)
                     /locus_tag="ACHBBW_001166"
     CDS             complement(18998..19477)
                     /locus_tag="ACHBBW_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9152866.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19604..21283)
                     /locus_tag="ACHBBW_001167"
     CDS             complement(19604..21283)
                     /locus_tag="ACHBBW_001167"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9152867.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21831..23333
                     /locus_tag="ACHBBW_001168"
     CDS             21831..23333
                     /locus_tag="ACHBBW_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152868.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23346..24008
                     /locus_tag="ACHBBW_001169"
     CDS             23346..24008
                     /locus_tag="ACHBBW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152869.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24109..24312)
                     /locus_tag="ACHBBW_001170"
     CDS             complement(24109..24312)
                     /locus_tag="ACHBBW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9152870.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24423..25607)
                     /gene="iadA"
                     /locus_tag="ACHBBW_001171"
     CDS             complement(24423..25607)
                     /gene="iadA"
                     /locus_tag="ACHBBW_001171"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9152871.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25764..27503)
                     /gene="yfcC"
                     /locus_tag="ACHBBW_001172"
     CDS             complement(25764..27503)
                     /gene="yfcC"
                     /locus_tag="ACHBBW_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9152872.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8226 a         5767 c         4673 g         8954 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga caactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa ctttttttct
     9301 atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac
     9361 ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt
     9421 gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg
     9481 aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca ctatcttttt
     9541 aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca
     9601 aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct
     9661 gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat
     9721 tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg
     9781 acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt
     9841 gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact
     9901 ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag
     9961 ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa
    10021 ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat
    10081 actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc
    10141 aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa
    10201 ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct
    10261 agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt
    10321 gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct
    10381 aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat
    10441 ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata
    10501 tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg
    10561 gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt
    10621 ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat
    10681 ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca
    10741 ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct
    10801 cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc
    10861 ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct
    10921 aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact
    10981 tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt
    11041 gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt
    11101 gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt
    11161 ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt
    11221 ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact
    11281 ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa
    11341 gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct
    11401 tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga
    11461 tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag
    11521 cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact
    11581 ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc
    11641 actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc
    11701 gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt
    11761 gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca
    11821 gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt
    11881 tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt
    11941 ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt
    12001 ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca
    12061 ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct
    12121 aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc
    12181 aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca
    12241 atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga
    12301 catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga
    12361 tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa
    12421 catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata
    12481 tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac
    12541 cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata
    12601 ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta
    12661 cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa aaatattatt
    12721 gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc gccttaaaaa
    12781 caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa ggtaacaaaa
    12841 aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac acagactgaa
    12901 atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa aaaggaggta
    12961 tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt cattcaaaaa
    13021 aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg gacaatcaaa
    13081 gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga tgaatttatt
    13141 tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa aaagttagct
    13201 gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa ctatttgatg
    13261 ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga acaactttat
    13321 atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt aataaaattc
    13381 catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga gttatctatc
    13441 cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg gccattttcc
    13501 tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga aaaaatttca
    13561 aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa caaacaacag
    13621 gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt gcagcataca
    13681 catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga agagacagaa
    13741 acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca aatctctttt
    13801 cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat ttgtcgacaa
    13861 gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt agaaaataaa
    13921 ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact gttgggtgaa
    13981 tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca ttctagttct
    14041 tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa aaatggggaa
    14101 agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca aacagaaacg
    14161 aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa ttatcatctt
    14221 caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc atattcagaa
    14281 catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac ggagactttc
    14341 tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc ggttcatgaa
    14401 ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa ttcttgtcac
    14461 atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta gctcagatca
    14521 aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga acaataagat
    14581 caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca gtgctgctgc
    14641 aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc cttcactcaa
    14701 aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca attggaaaag
    14761 taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc agctaagcaa
    14821 tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg ctggaacaag
    14881 tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat gtccgcattc
    14941 atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt gagacaaact
    15001 caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt gttgaacacc
    15061 gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac cagtcacatt
    15121 gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg ctgacgcaat
    15181 tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa aaccatacat
    15241 tccataacca tacattttcg tttcactcct tttcgtttac tttattttat cattagtagt
    15301 aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa attccgattt
    15361 cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc cttctactaa
    15421 acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt ttactttcaa
    15481 gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg catctaatac
    15541 cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt tcccgcattc
    15601 tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt ctgggcaatt
    15661 tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt aacggaaaaa
    15721 gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt tcgaacgaag
    15781 tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc tttatagatt
    15841 tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg gcctcctcca
    15901 tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg tacgcggtaa
    15961 taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat cactccagga
    16021 agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag aatttcttgc
    16081 ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata aagataaccg
    16141 gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc acttgcatca
    16201 aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt ggagggatac
    16261 aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc cttaacgttc
    16321 atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact acagctactt
    16381 gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg atggtcgatt
    16441 ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga attagctgta
    16501 tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg catttctgct
    16561 aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc tgggaaactt
    16621 tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc agggcgcaaa
    16681 tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc ttgctgtact
    16741 gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg attgctttgg
    16801 aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt ctgccgattt
    16861 ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc aaataatgta
    16921 ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt ttttgtgcga
    16981 catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca ttgaccattt
    17041 gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca cggtcagtat
    17101 caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc tgcggttgtc
    17161 gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct tccaatgtca
    17221 agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga tggctaacag
    17281 aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc cgatcgatga
    17341 tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc aaacgatgat
    17401 ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc tgataatcag
    17461 aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag atatcactga
    17521 caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc atggttgcga
    17581 ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca gctagctttg
    17641 caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca tcttccaatg
    17701 ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc gtaatatagc
    17761 gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc ttgtctaatc
    17821 ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca aatgtcaaac
    17881 gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt tgaattgttg
    17941 aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct tcactgacat
    18001 tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa ctttcaatat
    18061 attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag cgtttccgag
    18121 acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc gggatcttgt
    18181 tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg aattgtttgc
    18241 gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg caagaaagcg
    18301 acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc agtatcccct
    18361 gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc gcccatatac
    18421 tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa ttttaccacg
    18481 ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac tcctctgctg
    18541 ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc ttttgcaata
    18601 ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca atctgtcttt
    18661 acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc aatcatcacg
    18721 cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt aattttcata
    18781 ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat cacttgcaca
    18841 tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac agtatgtaca
    18901 aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata atctcaagtt
    18961 tgaggtctct gctctgctta gcttctcttc tcgtccctta actaattaaa tcagcgtaaa
    19021 taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc ccgatttcac
    19081 cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa aaacaataat
    19141 caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat cctgtatgca
    19201 ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact tgtctcgttt
    19261 ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca gttttgtttc
    19321 ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt tgtagttctt
    19381 ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg atcttttgtt
    19441 cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct ttattctatc
    19501 gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc aaaaaaagag
    19561 caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt ttatttcgac
    19621 gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt tgttcaaata
    19681 agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca aaatcattgc
    19741 tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc atcagatcac
    19801 ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa atgattttct
    19861 tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac aagatttttc
    19921 gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc attacatttt
    19981 ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc acgtctccgc
    20041 gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa tctgcatgag
    20101 cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc atcaaattga
    20161 tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc tcagagatca
    20221 acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg atggtttcca
    20281 tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc ttgacattac
    20341 ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt cggccagttt
    20401 ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta acaagcagat
    20461 cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt cgttcgaaat
    20521 cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat acttgttgca
    20581 ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa tatttgatcg
    20641 tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga ttttctgcat
    20701 ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc aatcgtgcat
    20761 aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc gtataaacga
    20821 tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta tgagtggtac
    20881 ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct accacatgga
    20941 aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct aactcgatcg
    21001 tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga agtgccccga
    21061 tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg ttttcttcta
    21121 aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca ggatatttta
    21181 gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg ttttttccat
    21241 tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc acctcttctt
    21301 atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg agccattcca
    21361 atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg aaaattacca
    21421 gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt cattaacaaa
    21481 atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag ctgttcccca
    21541 ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac aactatgcac
    21601 gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca tttcttcttc
    21661 gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact atagtgactt
    21721 tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt aagctagctt
    21781 taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat atgaatttta
    21841 tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta ttgtttgccg
    21901 tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc gctgcagaag
    21961 aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt gccaatcgag
    22021 aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact gaagaacgtc
    22081 cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa aaaatagctg
    22141 aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg gcaaaagatg
    22201 gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact actgatgcag
    22261 gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca gactttcaaa
    22321 tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca gcaaaaatca
    22381 cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc attgattcta
    22441 ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg agtactgaag
    22501 atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc gaaacaagcg
    22561 gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac acttcttaca
    22621 cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct gctatttaca
    22681 ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag ctgatcgaca
    22741 cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg gaacctttaa
    22801 acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc ggtattatca
    22861 tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta ggcgtcttgc
    22921 tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa gtggctattt
    22981 gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg aatgccgtgg
    23041 gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa gctcctggcg
    23101 gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt caacaaggaa
    23161 ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc aatgtcaaac
    23221 ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt tctgtctttt
    23281 tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac taaaggagga
    23341 atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa gatgcgcttt
    23401 ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta ggagcaagtg
    23461 gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca aaagaaggaa
    23521 aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat cgtaaaaatg
    23581 atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct ctagacaacg
    23641 tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa gttgctttag
    23701 atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg accaaacttt
    23761 cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa catcaactaa
    23821 tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat atcgtcaaac
    23881 ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact catgagattg
    23941 aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt caaaaaatcg
    24001 aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg tctcgcagat
    24061 atggttcgga ttttttattt gcttgattca attagtaata agtggttttt attttttttc
    24121 gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt tttctttgat
    24181 gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt ctgttgcttt
    24241 gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag aacgtccttt
    24301 taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt cagggtaaca
    24361 tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt tagagctgtt
    24421 gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg gaccattatt
    24481 tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat acacagatct
    24541 gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg tgctggatta
    24601 ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac agcttctttg
    24661 atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg attgttttca
    24721 tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc taaagaaaca
    24781 tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc tgtcttctcc
    24841 caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt ggcaaatgcc
    24901 acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa ttgtgtgatc
    24961 ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt tcctccgcct
    25021 aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg agcatctgct
    25081 actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga tactgcgatt
    25141 tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga atctgttact
    25201 gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc ttctgcttcc
    25261 agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc tgttcccaaa
    25321 caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac ttctggcgtt
    25381 cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca gtcaatgaaa
    25441 cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc ttcgtagccg
    25501 ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac atccattttt
    25561 cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca tttttcctct
    25621 ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata ggatctttac
    25681 tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt ttggattact
    25741 tttttattta caggtctcca gtactactgc agacctattg caatggcaat catgatgaaa
    25801 atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc ccattttgcc
    25861 cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg tgtaaaagcg
    25921 tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga tacacctact
    25981 aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga atttgatgta
    26041 acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat gacagctgga
    26101 acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga aggaacagat
    26161 gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac gattgctgta
    26221 ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac tttcattcca
    26281 tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa gaattgagaa
    26341 gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca gacaaggaca
    26401 actaacatct ctaataagat caatttatgc ccaagcatga attttttatt ttcatcagat
    26461 gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga actttctggt
    26521 tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc agtaacaact
    26581 acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc aatcccttga
    26641 gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg agatgcgaca
    26701 taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa tggaaccatc
    26761 accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc gaatgaaaaa
    26821 gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact gataaacgca
    26881 tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc tccaacaaca
    26941 aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac tagtccttca
    27001 aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat tttggctgtt
    27061 ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta aagcacatca
    27121 tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc gtcagcaatg
    27181 actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg atgatcttgc
    27241 agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata acggaaggaa
    27301 tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta ttcaatatct
    27361 tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa gatgactaca
    27421 ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg tttcggtttt
    27481 cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt aattttgatg
    27541 aataaaaaat ggataatcaa atgaattata cagtaaaaat gacatttcca catgaataat
    27601 ttattagaaa atcacttttt
//
LOCUS       DAYYKC010000027        27591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000027 DAYYKC010000000
VERSION     DAYYKC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.027"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..722
                     /locus_tag="ACHBBW_001173"
     CDS             75..722
                     /locus_tag="ACHBBW_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9152873.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            806..1750
                     /locus_tag="ACHBBW_001174"
     CDS             806..1750
                     /locus_tag="ACHBBW_001174"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9152874.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1885..3336
                     /locus_tag="ACHBBW_001175"
     CDS             1885..3336
                     /locus_tag="ACHBBW_001175"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9152875.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3357..4193
                     /locus_tag="ACHBBW_001176"
     CDS             3357..4193
                     /locus_tag="ACHBBW_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9152876.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4193..4852
                     /locus_tag="ACHBBW_001177"
     CDS             4193..4852
                     /locus_tag="ACHBBW_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9152877.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4978..5667
                     /locus_tag="ACHBBW_001178"
     CDS             4978..5667
                     /locus_tag="ACHBBW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152878.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5673..6629
                     /locus_tag="ACHBBW_001179"
     CDS             5673..6629
                     /locus_tag="ACHBBW_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9152879.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6673..7185
                     /locus_tag="ACHBBW_001180"
     CDS             6673..7185
                     /locus_tag="ACHBBW_001180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9152880.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7441..7989)
                     /locus_tag="ACHBBW_001181"
     CDS             complement(7441..7989)
                     /locus_tag="ACHBBW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9152881.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8078..9259)
                     /locus_tag="ACHBBW_001182"
     CDS             complement(8078..9259)
                     /locus_tag="ACHBBW_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9152882.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9495..11003)
                     /locus_tag="ACHBBW_001183"
     CDS             complement(9495..11003)
                     /locus_tag="ACHBBW_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9152883.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11162..11353
                     /locus_tag="ACHBBW_001184"
     CDS             11162..11353
                     /locus_tag="ACHBBW_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152884.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11556..12473
                     /locus_tag="ACHBBW_001185"
     CDS             11556..12473
                     /locus_tag="ACHBBW_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9152885.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12466..13539
                     /locus_tag="ACHBBW_001186"
     CDS             12466..13539
                     /locus_tag="ACHBBW_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152886.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13687..14205
                     /locus_tag="ACHBBW_001187"
     CDS             13687..14205
                     /locus_tag="ACHBBW_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152887.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14186..14578
                     /locus_tag="ACHBBW_001188"
     CDS             14186..14578
                     /locus_tag="ACHBBW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9152888.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14654..15589
                     /locus_tag="ACHBBW_001189"
     CDS             14654..15589
                     /locus_tag="ACHBBW_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9152889.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15722..16501
                     /locus_tag="ACHBBW_001190"
     CDS             15722..16501
                     /locus_tag="ACHBBW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9152890.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16494..16811
                     /locus_tag="ACHBBW_001191"
     CDS             16494..16811
                     /locus_tag="ACHBBW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9152891.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17055..18665
                     /locus_tag="ACHBBW_001192"
     CDS             17055..18665
                     /locus_tag="ACHBBW_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9152892.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18822..19670
                     /locus_tag="ACHBBW_001193"
     CDS             18822..19670
                     /locus_tag="ACHBBW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9152893.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19901..20380
                     /locus_tag="ACHBBW_001194"
     CDS             19901..20380
                     /locus_tag="ACHBBW_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9152894.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20413..22641
                     /locus_tag="ACHBBW_001195"
     CDS             20413..22641
                     /locus_tag="ACHBBW_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9152895.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22770..24413
                     /locus_tag="ACHBBW_001196"
     CDS             22770..24413
                     /locus_tag="ACHBBW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9152896.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24486..25031)
                     /locus_tag="ACHBBW_001197"
     CDS             complement(24486..25031)
                     /locus_tag="ACHBBW_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9152897.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25054..25539)
                     /locus_tag="ACHBBW_001198"
     CDS             complement(25054..25539)
                     /locus_tag="ACHBBW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9152898.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25529..26341)
                     /locus_tag="ACHBBW_001199"
     CDS             complement(25529..26341)
                     /locus_tag="ACHBBW_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9152899.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26480..>27591)
                     /locus_tag="ACHBBW_001200"
     CDS             complement(26480..>27591)
                     /locus_tag="ACHBBW_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9152900.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9039 a         5025 c         5523 g         8004 t
ORIGIN      
        1 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
       61 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
      121 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
      181 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
      241 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
      301 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
      361 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
      421 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
      481 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
      541 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
      601 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
      661 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
      721 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
      781 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
      841 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
      901 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
      961 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
     1021 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
     1081 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
     1141 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
     1201 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
     1261 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
     1321 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
     1381 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
     1441 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
     1501 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
     1561 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
     1621 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
     1681 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
     1741 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
     1801 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
     1861 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
     1921 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
     1981 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
     2041 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
     2101 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
     2161 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
     2221 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
     2281 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
     2341 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
     2401 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
     2461 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
     2521 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
     2581 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
     2641 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
     2701 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
     2761 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
     2821 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
     2881 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
     2941 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
     3001 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
     3061 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
     3121 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
     3181 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
     3241 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
     3301 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
     3361 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
     3421 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
     3481 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
     3541 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
     3601 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
     3661 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
     3721 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
     3781 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
     3841 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
     3901 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
     3961 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
     4021 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
     4081 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
     4141 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
     4201 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
     4261 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
     4321 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
     4381 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
     4441 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
     4501 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
     4561 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
     4621 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
     4681 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
     4741 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
     4801 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
     4861 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
     4921 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
     4981 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
     5041 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
     5101 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
     5161 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
     5221 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
     5281 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
     5341 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
     5401 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
     5461 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
     5521 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
     5581 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
     5641 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
     5701 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
     5761 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
     5821 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
     5881 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
     5941 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
     6001 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
     6061 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
     6121 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
     6181 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
     6241 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
     6301 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
     6361 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
     6421 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
     6481 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
     6541 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
     6601 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
     6661 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
     6721 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
     6781 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
     6841 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
     6901 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
     6961 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
     7021 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
     7081 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
     7141 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
     7201 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
     7261 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
     7321 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
     7381 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
     7441 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
     7501 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
     7561 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
     7621 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
     7681 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
     7741 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
     7801 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
     7861 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
     7921 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
     7981 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
     8041 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
     8101 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
     8161 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
     8221 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
     8281 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
     8341 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
     8401 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
     8461 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
     8521 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
     8581 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
     8641 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
     8701 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
     8761 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
     8821 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
     8881 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
     8941 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
     9001 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
     9061 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
     9121 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
     9181 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
     9241 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
     9301 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
     9361 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
     9421 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
     9481 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
     9541 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
     9601 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
     9661 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
     9721 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
     9781 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
     9841 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
     9901 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
     9961 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    10021 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    10081 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    10141 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    10201 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    10261 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    10321 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    10381 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    10441 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    10501 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    10561 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    10621 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    10681 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    10741 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    10801 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    10861 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    10921 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    10981 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    11041 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    11101 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    11161 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    11221 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    11281 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    11341 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    11401 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    11461 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    11521 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    11581 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    11641 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    11701 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    11761 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    11821 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    11881 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    11941 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    12001 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    12061 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    12121 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    12181 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    12241 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    12301 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    12361 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    12421 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    12481 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    12541 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    12601 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    12661 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    12721 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    12781 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    12841 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    12901 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    12961 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    13021 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    13081 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    13141 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    13201 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    13261 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    13321 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    13381 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    13441 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    13501 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    13561 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    13621 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    13681 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    13741 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    13801 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    13861 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    13921 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    13981 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    14041 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    14101 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    14161 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    14221 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    14281 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    14341 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    14401 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    14461 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    14521 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    14581 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    14641 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    14701 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    14761 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    14821 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    14881 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    14941 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    15001 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    15061 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    15121 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    15181 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    15241 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    15301 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    15361 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    15421 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    15481 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    15541 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    15601 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    15661 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    15721 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    15781 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    15841 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    15901 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    15961 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    16021 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    16081 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    16141 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    16201 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    16261 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    16321 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    16381 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    16441 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    16501 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    16561 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    16621 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    16681 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    16741 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    16801 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    16861 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    16921 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    16981 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    17041 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    17101 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    17161 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    17221 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    17281 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    17341 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    17401 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    17461 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    17521 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    17581 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    17641 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    17701 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    17761 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    17821 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    17881 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    17941 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    18001 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    18061 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    18121 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    18181 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    18241 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    18301 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    18361 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    18421 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    18481 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    18541 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    18601 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    18661 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    18721 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    18781 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    18841 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    18901 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    18961 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    19021 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    19081 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    19141 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    19201 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    19261 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    19321 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    19381 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    19441 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    19501 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    19561 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    19621 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    19681 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    19741 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    19801 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    19861 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    19921 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    19981 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    20041 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    20101 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    20161 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    20221 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    20281 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    20341 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    20401 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    20461 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    20521 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    20581 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    20641 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    20701 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    20761 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    20821 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    20881 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    20941 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    21001 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    21061 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    21121 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    21181 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    21241 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    21301 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    21361 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    21421 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    21481 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    21541 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    21601 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    21661 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    21721 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    21781 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    21841 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    21901 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    21961 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    22021 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    22081 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    22141 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    22201 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    22261 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    22321 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    22381 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    22441 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    22501 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    22561 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    22621 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    22681 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    22741 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    22801 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    22861 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    22921 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    22981 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    23041 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    23101 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    23161 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    23221 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    23281 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    23341 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    23401 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    23461 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    23521 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    23581 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    23641 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    23701 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    23761 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    23821 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    23881 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    23941 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    24001 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    24061 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    24121 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    24181 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    24241 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    24301 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    24361 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    24421 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    24481 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    24541 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    24601 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    24661 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    24721 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    24781 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    24841 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    24901 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    24961 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    25021 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    25081 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    25141 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    25201 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    25261 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    25321 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    25381 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    25441 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    25501 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    25561 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    25621 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    25681 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    25741 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    25801 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    25861 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    25921 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    25981 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    26041 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    26101 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    26161 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    26221 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    26281 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    26341 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    26401 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    26461 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    26521 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    26581 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    26641 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    26701 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    26761 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    26821 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    26881 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    26941 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    27001 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    27061 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    27121 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    27181 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    27241 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    27301 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    27361 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    27421 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    27481 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    27541 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga a
//
LOCUS       DAYYKC010000028        26906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000028 DAYYKC010000000
VERSION     DAYYKC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.028"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1077
                     /locus_tag="ACHBBW_001201"
     CDS             73..1077
                     /locus_tag="ACHBBW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152901.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1078..2022
                     /locus_tag="ACHBBW_001202"
     CDS             1078..2022
                     /locus_tag="ACHBBW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152902.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2022..2984
                     /gene="opp4B"
                     /locus_tag="ACHBBW_001203"
     CDS             2022..2984
                     /gene="opp4B"
                     /locus_tag="ACHBBW_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9152903.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3011..3925
                     /locus_tag="ACHBBW_001204"
     CDS             3011..3925
                     /locus_tag="ACHBBW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9152904.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3951..5732
                     /locus_tag="ACHBBW_001205"
     CDS             3951..5732
                     /locus_tag="ACHBBW_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9152905.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6080..6766
                     /gene="rnc"
                     /locus_tag="ACHBBW_001206"
     CDS             6080..6766
                     /gene="rnc"
                     /locus_tag="ACHBBW_001206"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9152906.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6786..10367
                     /gene="smc"
                     /locus_tag="ACHBBW_001207"
     CDS             6786..10367
                     /gene="smc"
                     /locus_tag="ACHBBW_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9152907.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10376..11185
                     /locus_tag="ACHBBW_001208"
     CDS             10376..11185
                     /locus_tag="ACHBBW_001208"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9152908.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11196..12194
                     /gene="ftsY"
                     /locus_tag="ACHBBW_001209"
     CDS             11196..12194
                     /gene="ftsY"
                     /locus_tag="ACHBBW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9152909.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12381..14648
                     /gene="gshAB"
                     /locus_tag="ACHBBW_001210"
     CDS             12381..14648
                     /gene="gshAB"
                     /locus_tag="ACHBBW_001210"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9152910.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14737..15189)
                     /locus_tag="ACHBBW_001211"
     CDS             complement(14737..15189)
                     /locus_tag="ACHBBW_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9152911.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15339..16289
                     /locus_tag="ACHBBW_001212"
     CDS             15339..16289
                     /locus_tag="ACHBBW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9152912.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16282..17241
                     /locus_tag="ACHBBW_001213"
     CDS             16282..17241
                     /locus_tag="ACHBBW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9152913.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17238..17993
                     /locus_tag="ACHBBW_001214"
     CDS             17238..17993
                     /locus_tag="ACHBBW_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9152914.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18016..18969
                     /locus_tag="ACHBBW_001215"
     CDS             18016..18969
                     /locus_tag="ACHBBW_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9152915.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19613..19909)
                     /locus_tag="ACHBBW_001216"
     CDS             complement(19613..19909)
                     /locus_tag="ACHBBW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9152916.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20264..20620
                     /locus_tag="ACHBBW_001217"
     CDS             20264..20620
                     /locus_tag="ACHBBW_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9152917.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20630..21529
                     /locus_tag="ACHBBW_001218"
     CDS             20630..21529
                     /locus_tag="ACHBBW_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9152918.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22049..22777
                     /locus_tag="ACHBBW_001219"
     CDS             22049..22777
                     /locus_tag="ACHBBW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9152919.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22922..24949
                     /locus_tag="ACHBBW_001220"
     CDS             22922..24949
                     /locus_tag="ACHBBW_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9152920.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25057..26154
                     /locus_tag="ACHBBW_001221"
     CDS             25057..26154
                     /locus_tag="ACHBBW_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9152921.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26461..>26906
                     /locus_tag="ACHBBW_001222"
     CDS             26461..>26906
                     /locus_tag="ACHBBW_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9152922.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9017 a         4661 c         5684 g         7544 t
ORIGIN      
        1 acgctttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
       61 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
      121 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
      181 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
      241 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
      301 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
      361 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
      421 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
      481 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
      541 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
      601 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
      661 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
      721 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
      781 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
      841 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
      901 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
      961 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
     1021 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
     1081 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
     1141 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
     1201 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
     1261 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
     1321 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
     1381 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
     1441 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
     1501 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
     1561 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
     1621 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
     1681 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
     1741 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
     1801 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
     1861 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
     1921 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
     1981 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
     2041 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
     2101 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
     2161 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
     2221 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
     2281 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
     2341 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
     2401 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
     2461 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
     2521 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
     2581 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
     2641 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
     2701 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
     2761 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
     2821 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
     2881 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
     2941 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
     3001 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
     3061 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
     3121 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
     3181 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
     3241 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
     3301 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
     3361 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
     3421 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
     3481 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
     3541 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
     3601 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
     3661 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
     3721 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
     3781 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
     3841 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
     3901 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
     3961 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
     4021 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
     4081 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
     4141 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
     4201 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
     4261 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
     4321 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
     4381 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
     4441 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
     4501 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
     4561 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
     4621 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
     4681 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
     4741 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
     4801 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
     4861 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
     4921 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
     4981 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
     5041 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
     5101 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
     5161 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
     5221 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
     5281 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
     5341 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
     5401 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
     5461 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
     5521 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
     5581 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
     5641 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
     5701 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
     5761 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
     5821 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
     5881 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
     5941 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
     6001 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
     6061 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
     6121 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
     6181 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
     6241 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
     6301 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
     6361 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
     6421 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
     6481 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
     6541 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
     6601 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
     6661 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
     6721 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
     6781 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
     6841 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
     6901 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
     6961 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
     7021 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
     7081 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
     7141 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
     7201 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
     7261 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
     7321 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
     7381 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
     7441 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
     7501 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
     7561 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
     7621 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
     7681 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
     7741 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
     7801 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
     7861 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
     7921 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
     7981 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
     8041 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
     8101 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
     8161 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
     8221 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
     8281 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
     8341 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
     8401 aagcagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
     8461 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
     8521 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
     8581 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
     8641 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
     8701 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
     8761 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
     8821 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
     8881 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
     8941 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
     9001 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
     9061 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
     9121 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
     9181 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
     9241 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
     9301 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
     9361 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
     9421 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
     9481 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
     9541 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
     9601 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
     9661 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
     9721 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
     9781 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
     9841 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
     9901 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
     9961 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    10021 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    10081 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    10141 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    10201 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    10261 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    10321 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    10381 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    10441 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    10501 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    10561 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    10621 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    10681 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    10741 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    10801 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    10861 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    10921 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    10981 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    11041 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    11101 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    11161 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    11221 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    11281 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    11341 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    11401 gttttgaaac agcgatacga atcaccgaag cattacgtca agaagtgaaa ctcagaaatg
    11461 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    11521 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    11581 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    11641 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    11701 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    11761 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    11821 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    11881 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    11941 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    12001 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    12061 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    12121 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    12181 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    12241 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    12301 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    12361 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    12421 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    12481 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    12541 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    12601 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    12661 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    12721 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaaa
    12781 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    12841 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    12901 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    12961 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    13021 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    13081 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    13141 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    13201 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    13261 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    13321 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    13381 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    13441 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    13501 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    13561 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    13621 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    13681 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    13741 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    13801 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    13861 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    13921 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    13981 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    14041 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    14101 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    14161 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    14221 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    14281 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    14341 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    14401 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    14461 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    14521 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    14581 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    14641 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    14701 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    14761 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    14821 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    14881 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    14941 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    15001 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    15061 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    15121 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    15181 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    15241 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    15301 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    15361 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    15421 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    15481 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    15541 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    15601 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    15661 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    15721 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    15781 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    15841 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    15901 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    15961 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    16021 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    16081 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    16141 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    16201 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    16261 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    16321 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    16381 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    16441 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    16501 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    16561 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    16621 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    16681 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    16741 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    16801 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    16861 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    16921 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    16981 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    17041 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    17101 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    17161 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    17221 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    17281 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    17341 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    17401 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    17461 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    17521 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    17581 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    17641 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    17701 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    17761 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    17821 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    17881 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    17941 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    18001 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    18061 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    18121 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    18181 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    18241 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    18301 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    18361 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    18421 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    18481 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    18541 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    18601 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    18661 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    18721 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    18781 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    18841 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    18901 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    18961 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    19021 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    19081 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    19141 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    19201 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    19261 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    19321 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    19381 ccagcgttct tattactcaa aacaaatcgc agttgtcaca atctcttcta aacgatgttc
    19441 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    19501 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    19561 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    19621 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    19681 gcttactcca ccgttttagt aattgataga aataactctc ttctgctttg attttatact
    19741 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    19801 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    19861 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    19921 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    19981 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    20041 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    20101 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    20161 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    20221 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    20281 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    20341 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    20401 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    20461 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    20521 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    20581 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    20641 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    20701 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    20761 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    20821 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    20881 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    20941 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    21001 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    21061 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    21121 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    21181 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    21241 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    21301 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    21361 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    21421 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    21481 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    21541 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    21601 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    21661 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    21721 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    21781 cagtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
    21841 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
    21901 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
    21961 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
    22021 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
    22081 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
    22141 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
    22201 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
    22261 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
    22321 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
    22381 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
    22441 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
    22501 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
    22561 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
    22621 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
    22681 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
    22741 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
    22801 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
    22861 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
    22921 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
    22981 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
    23041 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
    23101 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
    23161 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
    23221 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
    23281 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
    23341 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
    23401 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
    23461 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
    23521 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
    23581 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
    23641 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
    23701 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
    23761 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc ggattatacc
    23821 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
    23881 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
    23941 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
    24001 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
    24061 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
    24121 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
    24181 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
    24241 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
    24301 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
    24361 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
    24421 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
    24481 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
    24541 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
    24601 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
    24661 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
    24721 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
    24781 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
    24841 ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa gaatcggaac
    24901 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
    24961 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
    25021 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
    25081 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
    25141 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
    25201 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
    25261 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
    25321 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
    25381 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
    25441 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
    25501 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
    25561 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
    25621 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
    25681 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
    25741 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
    25801 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
    25861 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
    25921 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
    25981 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
    26041 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
    26101 ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa
    26161 gataacaaat agtaaatttt tattattgta ggatggttgg gctcttttta taacaatata
    26221 aaatatacca ttttattaat aaaaatgcat actagtaaat gatataattc taataacagg
    26281 tttgatcgcg agaaaggcca aataaaacgc tctccaaaat gttaaaaaaa agacaggacg
    26341 atgtatatta tacaaacaag aatcgctgac aacagtgact cggatccagg aaatcagttt
    26401 tatccccagc tagttggtga atgcatggaa agtaatatac gtagaaagag ctgatgacta
    26461 atgaattatg agtttttttt aagcaaagaa gatcacagaa gttattcatt acttaaatat
    26521 ttagaagcaa gctcaaattt atcagagtca atatcgaatg ttcaagaaga gttgtcgcta
    26581 tcgacatttc tcttgaaaaa aacgatcgat aagctgaaac atgatttaca aaagttcgat
    26641 ctagagaaaa atttgcgagt aaccgtcagt gaattggata tatgtctgga aattaatgga
    26701 aaatgttcta gcaaagctct gttatctcgt tatataacgg attctttatc tatgaaaatg
    26761 gtattggctt tctttcagga aaaatatatc tcagtagaaa attttgctaa acagcatcac
    26821 gtgagttatt ctgtggctta taaagtatta caaagactaa aaagaaatct aaaaaaatat
    26881 caaatttttt ttgaaaagag aaagtt
//
LOCUS       DAYYKC010000029        26784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000029 DAYYKC010000000
VERSION     DAYYKC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.029"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            578..1306
                     /locus_tag="ACHBBW_001223"
     CDS             578..1306
                     /locus_tag="ACHBBW_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9152923.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1324..2517
                     /locus_tag="ACHBBW_001224"
     CDS             1324..2517
                     /locus_tag="ACHBBW_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9152924.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2510..3505
                     /gene="lacD"
                     /locus_tag="ACHBBW_001225"
     CDS             2510..3505
                     /gene="lacD"
                     /locus_tag="ACHBBW_001225"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9152925.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3502..4443
                     /locus_tag="ACHBBW_001226"
     CDS             3502..4443
                     /locus_tag="ACHBBW_001226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9152926.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4489..6255
                     /locus_tag="ACHBBW_001227"
     CDS             4489..6255
                     /locus_tag="ACHBBW_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM9152927.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6272..6751
                     /locus_tag="ACHBBW_001228"
     CDS             6272..6751
                     /locus_tag="ACHBBW_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9152928.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6764..7654
                     /locus_tag="ACHBBW_001229"
     CDS             6764..7654
                     /locus_tag="ACHBBW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9152929.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7641..8450
                     /locus_tag="ACHBBW_001230"
     CDS             7641..8450
                     /locus_tag="ACHBBW_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9152930.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8466..8870
                     /locus_tag="ACHBBW_001231"
     CDS             8466..8870
                     /locus_tag="ACHBBW_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9152931.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(9051..9989)
                     /gene="yidC"
                     /locus_tag="ACHBBW_001232"
     CDS             complement(9051..9989)
                     /gene="yidC"
                     /locus_tag="ACHBBW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9152932.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10323..11405)
                     /locus_tag="ACHBBW_001233"
     CDS             complement(10323..11405)
                     /locus_tag="ACHBBW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9152933.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11547..12998
                     /locus_tag="ACHBBW_001234"
     CDS             11547..12998
                     /locus_tag="ACHBBW_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9152934.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12991..13713
                     /locus_tag="ACHBBW_001235"
     CDS             12991..13713
                     /locus_tag="ACHBBW_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9152935.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13733..14884
                     /locus_tag="ACHBBW_001236"
     CDS             13733..14884
                     /locus_tag="ACHBBW_001236"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9152936.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15232..15507)
                     /locus_tag="ACHBBW_001237"
     CDS             complement(15232..15507)
                     /locus_tag="ACHBBW_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9152937.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15615..16379
                     /locus_tag="ACHBBW_001238"
     CDS             15615..16379
                     /locus_tag="ACHBBW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9152938.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16503..17006
                     /locus_tag="ACHBBW_001239"
     CDS             16503..17006
                     /locus_tag="ACHBBW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9152939.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17271..17918
                     /locus_tag="ACHBBW_001240"
     CDS             17271..17918
                     /locus_tag="ACHBBW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9152940.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17961..18028
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            18079..19347
                     /locus_tag="ACHBBW_001241"
     CDS             18079..19347
                     /locus_tag="ACHBBW_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9152941.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19524..20726)
                     /locus_tag="ACHBBW_001242"
     CDS             complement(19524..20726)
                     /locus_tag="ACHBBW_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9152942.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21253..21978
                     /locus_tag="ACHBBW_001243"
     CDS             21253..21978
                     /locus_tag="ACHBBW_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM9152943.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21968..22642
                     /locus_tag="ACHBBW_001244"
     CDS             21968..22642
                     /locus_tag="ACHBBW_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM9152944.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22930..23214)
                     /locus_tag="ACHBBW_001245"
     CDS             complement(22930..23214)
                     /locus_tag="ACHBBW_001245"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM9152945.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23508..24815)
                     /locus_tag="ACHBBW_001246"
     CDS             complement(23508..24815)
                     /locus_tag="ACHBBW_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9152946.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25304..26410
                     /locus_tag="ACHBBW_001247"
     CDS             25304..26410
                     /locus_tag="ACHBBW_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152947.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         8632 a         4645 c         5597 g         7910 t
ORIGIN      
        1 tctatatttt tttatacaaa aaagatgttg ataaagttca caaacatcga aaagtataga
       61 gcccgaattt tacgaatatt ttttgtaata taaaagtata gatactaaat tcttattcgt
      121 taaacttacg ccataaggaa actagaaaaa gacagaatta tcataaaaaa tttttgttat
      181 ttttaaaaat gtacaacaaa atttcgacta tcagaccggc atgagagaaa agttatctaa
      241 aaaataaaaa atcctacttt atttagagaa agccattttt atttacatag tagtctatta
      301 ggcccatttg cttgtcgata gtagaaatag ccacagatgt ataaagaata ttttttcttt
      361 acttctctag accatttagc tgaatctttg ccaagattca gctttttttt gttttttatt
      421 tgtcaactgg tatttttgag gtatcttttg ataccagttc taaattacct tgaaaccttg
      481 ttataatagc ttttttacgc caaaaaccct ttgacaaatg aggaatatgt gggtattctt
      541 gtgttgtggt tataaccagt aaagaagagg tatttttatg gagagctcaa gtaaaggaaa
      601 atcattatat tatcaactag ttgacgtatt gaaagaacgt atcgaaacac agatgacacc
      661 tcatgacaaa ttgttatctg aaagagaact ttgtcagcat tatggagtga gtcggacaac
      721 ggttcgcctt gctttgcaag aattagagac gcaagggtat atttatcgcc gacacggaaa
      781 aggaacatat gtgtctgatc tatcagataa gtcgtctgat ctggcgggag cgtatagttt
      841 tacagaagag atgagggaat tgggacgggt gcctaataca gtcatcttgg aattcaagca
      901 aatggaagca aacaaaaagg ttgccaagaa tctaaacatc aatttaggcg aaacagtttt
      961 caaattgaaa agacttcgtt tagcggatga cgaaccattg atggtggaac gttcttattt
     1021 acctgcaaaa ctatttattg gtcttgatct gccattactt gaaagtaagc cattgtatga
     1081 tgtgttttct caggattatg aacagcaagt ccaattagca gatgaagaat tttatgcaag
     1141 tatcgttcaa aagaaagatg ctgatccgct tcgtatcgct gatggtgccc cggtattata
     1201 tctaagccga acgacctata atgtgaaaaa tgaaattatt gaatatacgg atagtgtagc
     1261 gagagcagac cagtttcatt ataagatccg ccacgtccga aaatgattga gagggaaaac
     1321 aacatgtttg aactatcgaa ggataaatta aaagagttgg gtgcggaaat aactacgcga
     1381 gaaatcaaac agcaaccgca attatggaaa gaaacagctg agatattagc acagaatcaa
     1441 gaaaaattag cattgttttt tgaccagata aaaaaagcag caaatggcaa aagagttcga
     1501 gtgattttta ctggtgcagg cacatcacaa tatgtcgggg atacagcagt accttattta
     1561 aatgaatacg gtgataacga tcactttatt tttcatagct ttggtacgac agatatcgtg
     1621 tcttcaccaa aagaatttct atttccggat gaaccgacat tactggtgtc atttgcacgt
     1681 agtggaaata gtccagaaag tgtggcaaca gtcaagattg ccaatgaaca aatcaagaca
     1741 ttgtttcatt tgacgattac ttgtgctaaa gaaggtcagt tagcaaagaa tgcagcgaca
     1801 gatgaacgaa attatctttt actgatgcca gaaaaatcaa atgatgcagg atttgccatg
     1861 acaggaagtt ttacgtgcat gttgttgagt gcgttatggg tattcgacca aactgccgtt
     1921 gagaaaaaag aaagttatct tcaacaattg atcagaactg gagaagatct aatttcacga
     1981 gatcaagaaa tccaaaaatt attgcctaag tcatttgaac gtatcgccta tcttggttca
     2041 ggaagtttgg ctggtgcaac gagagaagca caattgaaaa tattggaatt gacagccggt
     2101 aaagtggcaa cgatctttga ttcttctatg ggcttccgcc atggaccaaa atcgttttta
     2161 aatagcgaaa cacttgttat tggttttgtt aataaccaac cgtatacaag aaattatgac
     2221 ttagatattc tagaagaaat aaaagatgac caaattacca cgagtgtcat cgcgatcgcc
     2281 caaccaggaa acatcgattt cacaggtaca agtcttttgt tagaagcttc gatcagccta
     2341 cctgatgcgt atcttgcctt acctatgatc atgacagccc aaatcattgc gctcgatacg
     2401 tcgataaaag tagcgaataa accagataca ccatcgccta caggtacagt caatcgagtg
     2461 gtcaaaggtg tcattatcca tgaatatgtg aacaaagggg aaacgaagga tgaatgaaga
     2521 aaaaattgca gctatgaaga gtttatcaac aaaagaagga atcatcagtg cattggcaat
     2581 tgatcaacgc ggtgctttga aaaagatgat caaagcgtta ggaaaagaac caacggcgca
     2641 acagatcact gaattcaaag aattagtatc agcagaatta acgccttatg cttctgctat
     2701 tttactagat ccagagtatg gattgccagc tgctaaagcc agacatcagg aagcaggact
     2761 acttttagcc tatgaaaaaa cgggttatga tgcgacaact ccaggacgtc tgcctgattt
     2821 attagcggat tggtctacgc ttagattaaa ggaacaaaca gccgatgcga tcaagttttt
     2881 gctttattat gatgtagatg aagacccaga gatcaatcat ttgaaacatg tatttatcga
     2941 acgtttaggc agtgaatgtg caggtgaaaa gcgtccgttt tatctagaat tggtttcgta
     3001 tgatgccaaa accaatgaca gcacttcatt agaatatgcg aagatcaaac cgcataaagt
     3061 gatcgagatg atgaaagaat tttctgatcc taaatataaa gtggatgtat tgaaagtaga
     3121 agtgccagtc aatatgaact atgtagaagg ttattctgat ggtgaaattg cgtatagtaa
     3181 aaaagaagca gcggcattct tcaaggaaca aagtgatgca acaccgttac catttatctt
     3241 cttgagtgca ggggtttcga ctgcattgtt ccaacaaaca ttgatctttg ctcatgaatc
     3301 tggttcaaca ttcaacggtg tgttatgcgg acgtgcgact tggaaagatg gagtcaaagc
     3361 atatgttgaa aatggtagag aaggtgcgat tgagtggttg agaacacaag ggcgacaaaa
     3421 tatcgaatcg ttgaataaag tgataaatga aaacgccacc ccttggtttg acaaagtaaa
     3481 agaagattcg gtgacagcaa aatgatcacg acagttacca tgaatccttc aattgacatc
     3541 gcttacacgt tggatgaatt acggatcgac caagtcaatc gggtcaaaac ggtcaacaaa
     3601 acggctggag gaaaaggctt gaatgttgct cgtgtccttc atttattagc agatgatgtt
     3661 ttagctactg gattagtcgg cgggcagcat ggagcatata tcaaagaaca actcgatcaa
     3721 tcagggataa cgcatcaatt tttacctatc aaaggggaaa cgagagattc gattgctatt
     3781 ttacatggag tgaatcaaac agaactattg gaaagcggtc ctgttgtgac ggaagaagag
     3841 caacataaat ttaccgagca ttttgcacga ctaatggaaa caactgatat ctttacgatt
     3901 tcgggtagtc tgcctcaagg attttctaaa agttattatg cagagttact tgccctaacg
     3961 aataaaaaag gaacacgagt attgttagat acttctggtc aaaccttgct tgaagtcttg
     4021 gaaggcaagg aaaaaccatt tttgatcaaa cctaatttgg aagaattagg agagttggtc
     4081 caccaaacaa tttcttcggc tacttcgtta gatgaaatcc aatcattatt ggatcatccg
     4141 ctttttgaag gaatcaattg gatcgttctt tcgctaggcg caaaaggagc aatcgctaaa
     4201 gtagggagac acttctatca ggttacgatt cctaaaatcg cggtggtgaa tcctgtgggg
     4261 tcaggagatt cgactattgc aggcttagca catgctctat caaagagcta tacgattgaa
     4321 gaaacacttg cgtttgcgat ggcctgtggg atggcgaatg cacaacaaga aaaaacagga
     4381 tggatcgatc ctgtgaaagt cgattatttt aggggggaaa tcaacgtcag aaagattcgt
     4441 tgattataaa attagaaagc ggtttctaaa aacgaggagg aaatttgctt gactagattt
     4501 gaaataaaag aagagttttt gttagacggt caacctttca aaattttatc tggtgccatc
     4561 cattatttcc gagtgcatcc gacgaattgg gagcggtcat tgcacaattt gaaagcgctt
     4621 ggatttaata ctgtggaaac ctatgtgcct tggaatctcc atgaaaaaga agaaggaacc
     4681 tatgattttt cgaaaatatt ggatctttcc cattttctag agttagcgca atcccttgga
     4741 ttatacgcaa tcgtacgtcc agccccttat atttgtgcag aatgggaatt tggtggcttg
     4801 cctgcttggc tattgacaaa ggagtgccgt attcgttcag cggatcctcg cttcatgaag
     4861 tatgtcaaac ggtattacca aacgctacta cctaaaatcg ttcctcatct agtcactcat
     4921 ggtggcaacg tcttaatggt gcaggtagaa aatgagtacg gttcgtatgg tgaagagaaa
     4981 gcatacctgc gccaagtaaa aaaatacttg gaggaaggtg gaatcgatgt accgctattc
     5041 acttctgatg gaccttggca agccacatta agagcaggca ttttgattga cgatgatgtt
     5101 tttacgacag ggaattttgg ctcaaaagca aaagaaaatt ttgcagatat ggcccagttt
     5161 tttgaagcac atggtaaaaa atggccgttg atgtgtatgg aattttggga tggctggttt
     5221 aatcgttgga aagagccgat cattaaaaga gatccggaag aattagcgga tgcagtcaaa
     5281 gaagcattac agatcggcag tatcaatctc tatatgttcc atggcggaac caattttggg
     5341 tttatgaatg gctgttcagc cagaggcgcg attgatttgc cgcaagtcac ttcctatgat
     5401 tacgatgcac cattggatga gcagggaaat ccaacggaaa aatattttgc tctgaaacga
     5461 atgatggcag aaaattttcc agaaatgcag caaatggaac catggataaa agaaacaatc
     5521 gtcaaaaaag gaatcaactt gaaagaaaaa gtcagctttt tcgcaacact ggataccatc
     5581 agcaagccga tccactcgac ctatcctcaa acaatggaag aattgggtca gaatactggg
     5641 tacctattgt atcgaacaac gatcaaaaaa gatgcggtca atgaaaaatt acggatcatc
     5701 gacggacgtg atcgggtcca tgtttttctc gatgaagaaa agcaagcgat tcaataccaa
     5761 acagagatcg gtgagaccat tccattgaca cttgagaaag aagaccatca aattgatcta
     5821 ttatttgaaa atatgggacg ggtcaactat ggacacaagc tgctagctga tacgcaaaga
     5881 aaaggaatac gtacaggagt gatgagcgat cttcatttta tcacagaatg gacacagtat
     5941 tgtttacctt tagagactac agaacacatc gatttttcaa aaaaatggga agcaggtcaa
     6001 ccagcatttt accgctttga agcggaactg tcagaaatcg gtgatactta tttggacctt
     6061 agtgagtttg ggaaagggat cgtttgggtg aatggcacga atattggtcg tttttgggaa
     6121 gtaggaccaa ttctttcact gtttgttccc gagggtttat ttagaaaagg acaaaatgaa
     6181 atcgttatct ttgaaacaga aggtagattc agtgaagtta tagattttgt caaagaacca
     6241 atagaaaaaa gttagaaaag gagaatggat gatggcaatt attggagtaa gaattgatgg
     6301 acgtttgata catggacaag tggcaaatct ttggacgaca aagttgaata ttggtcggat
     6361 catggtcgta gacgatgagg tagcaacaaa tgcgatcgaa aaaagtgggt taaagttggc
     6421 aacgccagct ggagtcaaat taagtatttt acctattgaa aaagcagccg caaatatttt
     6481 agcaggtaaa tatgattctc aacgagtgct gatcgtagcg aaaaaaccag atcgtatctt
     6541 agcgctagta gaaaaaggtg taccgatcaa agaattaaat gtcggcaata tgtcacaaac
     6601 agatgaaaca cgttccatca caaaatcaat caatgtcgta gatgcggatg tagaggtctt
     6661 caagaaatta gatcagcttg gcgttcattt gatcgctcaa atggtgccaa atgacaaagc
     6721 agaagacttt atgagtcttt taatcaaata ggaggaatga ttcatggata ttcaatggtg
     6781 gcagatatta ttattaacat tatatgcagg gtatcaaatt ttagatgaat tacagattta
     6841 ttcttcaatg agcgccccag tttttgcagg attatatgct gggttagtaa tgggtgattt
     6901 aaaagcaggt ttaatcattg gtggaagtat gcagttgact gttttagggg tagggacatt
     6961 tggtggtgca tcaaaaatcg atgccaactc tggaacgatc ttagcaacag ctttctccat
     7021 ttctctaggg atgaacccag aacaagcgat tgcggccatc gcggttcctg tagcaagttt
     7081 gatgatccaa ttagatattt tggcacgttt tgcgaatacg tattttgcac atcggatcga
     7141 tcaacatgtg gaaacaatga attacaaagc aattgaacgg aacttcttga tgggggcatt
     7201 accttgggca ctttccagaa tggttcctgt attcttggcc ctagcattcg gtggtggctt
     7261 agtccaaaat gttgtggctg ttttaaacgg cgacctaaaa tggctaggag atggcttatc
     7321 tgttgcaggg gcagtcttac cagcagttgg gtttgcaatt ttactacgct acctaccagt
     7381 aaagaaacac atttcttatc tgattttagg atttacgatc acagcgttat tagcaacagt
     7441 gttctcgaat gtccaattgt taggaacaag tgtggctagc gtggtcaaag atttcccagg
     7501 aacattcaat tcattaccga tgcttgctgt ggcattgatt ggtctagcac tggcaacgat
     7561 cagttataag aatgatcaaa agaatgacca acatccagta gtaagtcagc aatcacaatc
     7621 tgatgagggg gaaatcgaag atgacgaaat ctaattataa attgaccaaa gaagatttta
     7681 agcagatcaa tcgtagaagt ttgtttactt tccaattagg ttggaactat gaaagaatgc
     7741 aaggttcagg ttatctgtat actatcttgc cacaacttag aaaaatctat ggtgatgata
     7801 caccagaatt aaaagaaatg atgaaaacac atacgcaatt ttttaatacg tctaatttct
     7861 ttaacacgat cattactggg atcgatcttg ctatcgaaga aaaagaagga gtctcttcaa
     7921 aggaaactgt ttctgggatg aaagcaggat tgatgggttc cttcgctgca attggtgatt
     7981 ctgtatttgc ggcattgatc ccgaccatat ttggagcttt agccgcaaat atggcaacag
     8041 atggtaatcc agtaggtatc cttatctgga tccttgctca ggtcgctgtc atggtctttc
     8101 gttggaaaca actagaattt gcctacaaag aagggatttc tctagttacc acgatgcaat
     8161 cacgcttgac tgcattgaca gatgcggcta ctttactagg agttttcatg gttggagcgt
     8221 tagtagcaac catgatcaat gttaaattct catgggcacc aacgattggg gatgtaacgt
     8281 tgaatatgca aaactatatc gatatgatct tacctaaact tttaccagct ggaatcgttg
     8341 gtggcgttta ttggatgtta ggtaagaaaa atatgacacc tactcgagca atattcatcg
     8401 tattgatcgt atgtgtagca ttatctgcat taggtgtgat ttcgaaataa gggagaattg
     8461 gaaagatgag tagacaacta gtcttgatta gtcatggtaa attttgtgaa gaactaaaaa
     8521 acagtacaga aatgatcatg ggaccacaag aaaatatcta tacgatccct ttattgcctg
     8581 aagatggcgc ggacacgtat aaagaaagat tcctctccgt tacagcacca ttgaaagatt
     8641 acgtagtgct ttgcgatctg ttgggtggga cacctgcaaa taccgtttcc aagctaatca
     8701 tggaaggtga aacgatcgaa ctatatgctg gcatgaattt gccgatggtc atcgaattca
     8761 tcaattcgca aatgattgga gcagaaccag attttattgg ttcgggtcgt gagagtatcg
     8821 tttcagtgaa ccaagtgatt gctggcatgg gagacgatga agaagaataa tcaagcacaa
     8881 cttcttgact ggtgatacaa ctggatataa atacggatgt ataaagaggc cgtgtataaa
     8941 aaagccaacg taccgcgaaa tatagttttc tatatttcac agtacgttgg cttttatcgt
     9001 cataaaatca gaaatgatct gggtcgatcg aagaagctag ttacaaataa ctatttacgc
     9061 ttttgctgtt taccagcatt ccgaccattc ttcgcattgt tttttggtgc ttgtacttgt
     9121 ttttttgttt gcttcgcagg ttcagcaggt gtgacatctt tacgaggcgt caccacttgt
     9181 tttggcggat tttgttttaa ttcttccgca acttgcgctt ttaatcttgg tttcatgatg
     9241 acattcgtaa tgaatgtttg tagacaacta aagatcccac cgactaccca gtaaagagtg
     9301 acaccagctg gggcactgat cgacatgaag acgatcatag cgggtgatac gatcaacatc
     9361 gtacgcatcg ttttcttttg ttcttcaggg ataccgatag ttgagatata gccttgaagt
     9421 aaataagcaa taccggcaat cgctactaaa actaagctag ggttacctag gtcgatcccg
     9481 taaaaggttg attcacggat gccttctgta tagcgagcag tgaaatatag cgcagaaaag
     9541 atcggcattt ggatcagaag cggtaaacaa ccgataccac cagtcatact taagccattt
     9601 tcacgataaa cttgttgcat ttcagcttgt gcagccattt gttcttctcg agttgtcgca
     9661 gctttcaatt tttgttgcgt agcttcaact tgtggtttca atgcttgcat cttttcagat
     9721 tggatcattg tttttttcga ttgactaata cctaatggca aaatgatgat acgaacgatg
     9781 actgtcatca agataacagc ccagccgtag gaccaattaa agttttccac taaatatgta
     9841 atcgcttgtc ccattgggtg aaccaatata cggtaaatga ttcctgaagt gtcagggtta
     9901 ccatttgaat ccatgcggac acaaccggac aagaaaagca tgatcgtgac aagcccagag
     9961 cccaataacc aatttttcca tttattcata aaatacttcc tcttttataa tttttacaca
    10021 agcttaacta tactaaaaaa gcgctcataa ttcaatcaac ttcatagaaa tttcatagaa
    10081 ataaccgatt ggaggatcaa aaaagacgct taatgttaaa aagaagaatc cagctaaaaa
    10141 tacttcagta taaagcatac ttagcacctc cgttctctaa aataagatga gcaacggata
    10201 tttccttgaa atcataattt tagcgcaaaa aaaagaagtg gaatgtcaat tttttccact
    10261 tctttttttt taccattgag ccgatccata aaaaagctga ctgccatggt ataaggggct
    10321 aactatccct gtgtcacttg ttctaactct ttcattttct ggacaaccag tgctggtgtg
    10381 atcgtttctt tcatgaagtg gatcatttca tcaggtaaac atgcacgttc agctacggct
    10441 tgatagtcac tttctgttaa atgtaaaccc atatcagcta aactcgttgg taagttcaag
    10501 tgatgataaa acggcagtag gtgttcgatt tctgctaatt tattttcgat catcagctgg
    10561 acaaggatac cgtatgcaac tttgtttcca tgaagttgct gatggctatc agggagtacg
    10621 gtcatcgcat cgtgaatgga atgagctcca gctgttcggc cgtagtcatc gccaaaccca
    10681 ccaaccatac cagccaataa aatgtttgtt tccacgacat cgataaaagc ttgattgatt
    10741 ttttgctggt ccatcgcttc cattgcctct gcacttttct ctaaaaggat ttcgcggcat
    10801 cgtaacgctg caaattccgc tacttggact tcgataggct tgatagttaa ctgagagatg
    10861 attgcgtgag attcatacca ttttgctaag gtgtcaccaa taccggcaat catcatctcg
    10921 cgaggggagt gcaaaatgat ctttggatcg atcaacacta aatcatttgc tttagggaac
    10981 atatcatagc gaagcatcac tccttcttca ttatagacca cactaagtgg agtataagct
    11041 gcgcacgtcg cagctaaggt aggtaagatg atgattggta agtttgtctt ataggcaatc
    11101 aattttccta gatctgctac tttcccgccg ccgactgcaa taataccatt gaaatccttc
    11161 tttttgcata aagaaacaaa actgtcagca agtggatcgg tacattctcc accataatat
    11221 tcaaaaattt ttgttattga taaatctgga aaaacatctt ttactgcttt ccaagatgtt
    11281 tttccatgga gaatcaaaac gttgtcaata tgccttttgt aaaggtgttc agggagtgaa
    11341 tcccatgcgt tttcttttat ttcatactct tgaggggcac cgcgaacaat taaatttttt
    11401 gtcatataaa aaacttcttc cattattttt ttaaattaat ctttttttat tataatagtt
    11461 atgctagtat attagcaagt tatttatgaa tatttattct tttatagctt ttctgattac
    11521 attattattc aaaggtggcc aaatggatga aaaaaggaat aaacatatta ttagtggcgc
    11581 tgatgtttct actggttttt ttaccagtaa aagtacaagg tgcagaaaaa gatgaaattt
    11641 acgagcggat aaaacaaagt ggagaattag tcgttggcct atctgcagac tatgcgccct
    11701 atgaattcca tgcgaatgtg gatggtaagg ataaaatcgt tggttttgat gtatccattg
    11761 ccgaaaagat cgcaaaggat atgggtgtga aactaaaagt agaagaactt ggttttgatg
    11821 cattattagg tgcgttgaaa actggcaaga tcgatttagt tatatctggt atggcggtga
    11881 ctgaagaacg tttacaagaa gttgactttt ctgacccata tttcgtggta cagcaaaaag
    11941 tactcgttcg taaagaggac aaaaataaat ttaagacgac agccgacttt tctggcttag
    12001 ccgttggggc gcaaaagcaa accacacaag aggagttagt gaaaaatgaa ctgactggtg
    12061 cagagttgac ctccctgcaa aaagttcctg atctgatcaa taatttgctg aatgaaaaag
    12121 ttgaaggtgt tgtgctggaa caaccagttg cagaagcata tgcccaacag aataagaaag
    12181 tagctttgac tccagtaaaa tttgcgcaag gcgaaaaaac cacagctatt gcgatgtcaa
    12241 aaaatacacc agaactgaaa aatcatgtca atgcgtcaat caaaacgatc aatgacgacg
    12301 gcttgctgaa agattatcaa aaacaagcga atcaattgat gttccaagat gatcagtcct
    12361 ttcttcaaaa atacggtccg tattatttga ttggaacaaa atacactatt ttattagctt
    12421 ttgtgggtgt attgtttgga atggtctttg gtagtttgct tgcactgatg aaattgggta
    12481 agaataaatt tttaaaggca ttagccatta tttatatcga atacgtacgg ggaacacctc
    12541 tcttagttca aatctttatc gtttacttcg gaacaggagt actgggattg gatctatcga
    12601 aactggtagc aggttgtatt gctgtctcgt tgaatagtgc tgcttatgta gccgaaattg
    12661 tccgtgccgg gatcaatgcg gtcaacaaag gacagatgga agctgcacgt tctttaggaa
    12721 tggaccagaa agaagccatg cgtttcatca tcttcccaca agcaatcaaa aatattcttc
    12781 cagctctagg aaatgagttt gtcacgatca tcaaagagtc ctcagttgtt tcggtcattg
    12841 gggtatcgga attgatcttt caaacgggcg tggtacaagg tgctagcttc aaaccattcc
    12901 taccatatgt gttggtctct ttgatttatt tcgtgttgac cttcagtatt tcccgtatat
    12961 tagctttagt tgaaaggaga atgggtctga atgcttaatg taaaaaaact gtcaaaaagt
    13021 tttggcgaga atgaagtctt aaaatcaatt gatctttctg tgaaagaagg agaagtagtg
    13081 gtgatcattg gcccttcagg aagtgggaaa agtacaattc tacgttgtat gaatctgctg
    13141 gaagaaccaa cttctggtga aatctttttt aagggacaaa acattacagc ccctgacagc
    13201 gatatcaatc aaattcgtca aaaaatggga atggtatttc aaagttttaa tttgtttccc
    13261 catatgaatg tactcgaaaa tttgaccatc acacctgtaa aaatcaaaaa agaagaccct
    13321 aaaaaagcaa aagaacatgc tctatcatta ttgaaccaag ttggtctaaa ggaaaaagtg
    13381 gacagttatc ctaccagttt atctggggga cagcaacaac gggtagcaat tgctcgagca
    13441 ttagcaatga atcctgatgt gatgcttttc gatgaaccta catctgcact tgatcctgaa
    13501 atggtcggtg aagtgttggc agtcatgcag gatttagcta aaaaagggat gacgatggtc
    13561 gtggtcactc atgaaatggg ttttgctaaa gaagttgccg atcgtgtgat ttttatggct
    13621 gacggcatca ttcaagaaga gggaacaccg aaagagattt ttgaaaatcc gcaaaatagt
    13681 cgaacacagg atttcttgaa caaagtgttg taacaaagat ggaggaagta agatggacaa
    13741 aagtaagatt gcacgccggt ttcagcaacc agcagaaaat ctgttgatga atattgctat
    13801 tttggcaaag gaaaaagagg atattatcga tttatcgatt ggtgatcctg acttgatcac
    13861 tgatccgacg attattgaag cagcttatac agatgtaaaa aatggtgcta ctaattatac
    13921 tgcgcctgat ggcagcaaag aattcttgtc aacggtcgta gatttttatc aaaagaaata
    13981 tggattgagc gttgaatgca accaagttcg agctacagtc ggagcaatgc acgggatgta
    14041 tctagtttta caggcattac tagatccaga ggatgaggtc atcatccatg aaccttattt
    14101 ttcaccatat cgggaacaag tcattttatc tggaggcaag cctgtttttg ttcctaccta
    14161 tgaagaaaag ggcttccagt tggatgtgga agatttaaaa gcagcgatta cggatcgtac
    14221 aaaggtgatc gtcatcaatt cgccgaataa tcctacggga gctgtttttt ctgaaagcac
    14281 gttgaaaaaa atcgctgaag tcgcaatcga aaatgaactc tatatcattt ccgatgagat
    14341 ttatgaagac tttgccttta aagagaagtt cgtaccgatg gcgcgatttg cgcccgataa
    14401 tacgatcact gtcagtggtg tatcgaaggg gtttgcgatg actggctggc gcttagggta
    14461 catgatcgca ccagcataca tcaatcaaat tgctgggttg atcaatgaaa acatcactta
    14521 ttccgcaccg actgcttctc aaagaggtgc aatctacgcc ttaaagcact atgaacagct
    14581 tgtaccgcca gttgttgaag tattcaaaga acgattagct tatattgaga aaagagtggc
    14641 agaaatccct tacttatctt tatcttctgt ttctggaagc atgtatgctt ttattaatat
    14701 cagtaagagt gggctcgatt cagtaacgtt tgtggaagag gttttagaaa aaagcggcgt
    14761 actttttgtc cctggaaaag cgtttgggga aacgatcggg gacaactata ttcgactagc
    14821 agcgactcag ccattggaaa tcttaggtga agcctttgat cgtttagcaa aactaaattt
    14881 ttagataaaa ctgtatattt gaaaacagct atgggtatcc attagaacaa agagggaaaa
    14941 tgatcctttc gagagttggc ttgttgactg ggcagcgtca ggatttgaca atgaaaaagt
    15001 ggtagtggaa aatcaaagtc gattttccac taccataaaa tattatagac tccattttat
    15061 tgaaccaaaa tttttgaagt cctcccgctt ttacgtatgc ctatcactgg ttactaaact
    15121 cgctaataag aatgtttttt tgtcgatcta ggatggagag aggggcaaac tttttcgatc
    15181 tacatacaga cagtccgatt tttctgacat ttcctattca tttctagaag gtcagcctat
    15241 tacaacaaac ttttttctgt cctccatcat cggattttcc tgaaggtcta catattggac
    15301 acgtccgctg gggcttggtg atcgtttgac ttcttccacg aattttttga ttgcctcatc
    15361 gtcgcccgca gcttcgatcg tgacagagcc gtcatcttca tttttcactg ttccggtgat
    15421 ccctaattga tccgctacga tttttgtcat atagcgaaaa ccaacgcttt gtacacgtcc
    15481 ttgcacgttc atcttgattt tgttcatttt attcaccgtt ctttctttta tttttaaatt
    15541 aatgataccg acttttttgc tactgaacaa gtgcaatgat ctaatccatg gtataatcag
    15601 ttgaggtgaa aaatgtgaaa gagatccaat cagcaaaaaa tccaagaatc aaagaattaa
    15661 aaaaattaca taagaaaaaa tatcgtgaag aaaaagggga atatctgctt gaagggtttc
    15721 acctgatcga ggaggctgtt gcctcaaatg cgaaggtcaa cgaacttttt attacgcaac
    15781 gcggtctgac tgagtgggct agttggcttg aagaaaatca ggcgctttct gttacactag
    15841 tatcagaaga agtgatggca actgtttcag atctaccaac tccgcaaggg atcatggcag
    15901 tggtggaagc tgaaaaaaga gagataccga attttgaagg tggttggttg ttgctcgaca
    15961 acgtacaaga tcccggcaat gtagggacaa tgatccgcac agccgatgct tttggtctat
    16021 cttgtgttgt actaggtaag ggaacggcgg atgtctacag taccaaagta ttaagaagca
    16081 tgcaggggag taactatcat ttaccgatca tccaagagga tctaacaaaa ctattgccta
    16141 tccttaaaga gcaaggattt ataacctatg gaacagaact gaatgaagaa gcagtgtccc
    16201 tgaatgatat ccagacaaca gaaaattatg cgatcgtgat ggggaatgaa ggtcaaggcg
    16261 tacgtccaga agtgctggcg ctaacggata aaaatgtgta tataccgatt caaggaaatg
    16321 cggagtcttt aaatgtagga gttgcagcgg gcatcatgct ttatcacttc tccgtatagt
    16381 taaggaacta ttaagctttc tgcattttgc gaaaacctca ctagaatatt acgaacacta
    16441 gtggtatact aagttcaatt tcaaagataa atggaattca aaaaggtggc agatcgataa
    16501 ttatgacaga aaaatggcgt gaagacgaag aatatatgtc ttatataaat gatctattag
    16561 aaactgatgc agtaaagaaa ttagcaaatt acacgcaaca tgtccactcg acacgattag
    16621 agcattcaat cagtgtgtct tactatagct acaaattagc gaaaaaatgg ggcggtgatg
    16681 caagagcgac tgctcgagca ggccttttac atgatttatt ttactacgat tggcgtacga
    16741 cgaagttcga tgaaggtaca catgcgtatg ttcacccaag gatcgctgtg aaaaatgctg
    16801 aaaaaatcac ggagctttca gatctagagc gtgatattat tttgaaacat atgtggggag
    16861 caacgattgc ccctcctaaa tataaagaag gttatatcgt tacttttgtg gataaatatt
    16921 gtgcagtcaa agaagcagct caaccaatga cagcttcaat gaaacaaaaa tggcaacgat
    16981 atttcggtaa agaacaatcg atttaaggta gttaactaag tatttgaccc gagtattttg
    17041 aaaaaaatcc tcgggttttt tgtttagaca ttgactaatt ggtcttttta gtgtttactt
    17101 tgttgagatg aaaaagtaca taaccaacat aaaaataaag ggggtgatga taagatgcga
    17161 tgtgtatctt atttaaaaga gtttgagggc tttatctaaa ggcactttga tagaaagatc
    17221 acttttttct tcaaagtagc cgattttgaa agaagaaagg actcaaagtc atggaaaaaa
    17281 caatcaaacc atatcaatat tattttatca agcgagaagt cgagcagctg ttaagcgcct
    17341 atagttcagt gaatgatcca aagacagtcc aaacagtcca agcattagct gcggaaaaga
    17401 tccacgaggt acttggtcat cctaatacag ctgtaaatga atttatggaa aaggtactag
    17461 acgtcactct aaaaaaagaa aaagcagaac gactattaga ggaattgaaa gtgatcgttc
    17521 ttccattcat acaaccgaca aaaccgcaga tcgataaatt attccgaaaa gtgaaaaagt
    17581 taaaacaacc agattggaat caactggatt taagggaaca tacttatatt ggttggaatg
    17641 atccaggtag ccaaaaaaaa tttatgatct attatgaaga agaaaaacta aaagggatca
    17701 gtggaacatt atctcctaca atcaacaaag gaatttgttc gatttgtcaa agaacttcaa
    17761 atgtttcact ctttctttct accacaaaaa ctggttcaga tggaacatac acgaaaaaag
    17821 gaaattatat ttgtcatgat agtgaccaat gtaatcagca gttggtacaa ttagctcctt
    17881 tttacgaatt cacagaacga gtgaaacgtg agcggtgatt tcattgtatt tttctgaaaa
    17941 atatgctatc ttatttggtg aagtgaatag gtgatggtgt tcgcctttaa acgttgatcg
    18001 taagataact aatgacgcct actaaagaaa cggggctacc ccttttttta gtaggcgttt
    18061 ttttgaggag gaaatgagat ggaaaatcga ttacctgatc cattgaaggt gatcatctat
    18121 atgatcaaat ggttattgat cacagcaatt ttaggtattt ttatgggaag tttatccgca
    18181 ttctttctaa ctagtttgac tgatgtgaca gctattcggc tggagcatcc ttggttactt
    18241 tttcttttgc cgataagtgg gggacttttt gcttatctat atacgacgta tgggggcaat
    18301 tcttcccggg gaaacaattt agtgatcgat caagggaatg gaggaaatga aaagattcca
    18361 ttacggctga tcccactgac cttatttggc accattacca cgcatctttt tggtggttct
    18421 gttggtcggg agggtacagc ggtccaaatg ggcggggcac ttgctgagaa ttttgctcgt
    18481 tggctaaaat tgaacaaaat cgagcgtgag attcttgtca tcagtgggat cagcgcaggt
    18541 tttagctcag tatttgggac acccttggct gggacattgt ttggattaga agtattggca
    18601 atcggaaaag tgagaaccga agcactgttt ccaagctttt ttgcagcact ttttgcgaac
    18661 tttgtaacgg aaagtttcaa tgtcaagcat acccattatc aaatgggggc gattcctgca
    18721 tgggaccctt cacttttctt caaattgttg cttgcgggca ttgcatttgg tatcattggc
    18781 tggctcttta gccgttcgat cgtctggtta aaagcttggt atgcaaagcg gatcccccat
    18841 ccagttttac gcaactttat tggaggagca gtcgtggtac tcgtagtagt gattttacag
    18901 acgcaaagat atctcggatt aagcttacct ttattacaag atgcatttaa tggaagtgct
    18961 catccttatg attttcttgg gaaacttggc tttaccgttc tatcacttgg cgcgggatac
    19021 caaggaggcg aagtgacccc tttgtttgag attggggcaa ctttaggtgc gacattagca
    19081 cccattcttc atgtatcagt acctttttta gcaggattag ggtttattgg ggtgttctct
    19141 ggagcaacga atacaccgat tgcttgcttt gttatgggga tcgaactttt tggaggtcaa
    19201 gcagccagct acttctttat gatctgtttg attagcttta tgtgttcagg aaatagtgga
    19261 atctattctg cgcaaaaagt tgaagtgaaa aaaggaatct tgttcctacc gatccttgga
    19321 agaatctcac gacaaaaaga gaaataaaaa gaggttggga cagaagtgtt taactccgag
    19381 aaataagaag aaattcacga aaattgcttt ttaaattttt gtgaatttcg cttatttccg
    19441 aaggagttgc ttctgctccc accgtttatt cggagttaga gtctgtggca aaactgattt
    19501 ttagttttgt cccacgctct tttttattga gcaaatttga gaataagagc agaaaatact
    19561 gcaaagccaa tcattaagcc acagatgata agatggcgtt ttttgtgtaa gacaaggatg
    19621 gcaataaatt ctcttaagat agcattcggt aagtagtata gtgcagtttt tgcaccgatt
    19681 ccgtttgcat tgagtttcgc ttgtcgcgca tatagtccag ctctaaagat atggtaatta
    19741 tttgaagtaa aaatcgcgcg ataaggccca ttcatttgct gatccattat ttcttttgaa
    19801 aacagcatat tttctagcgt cgttgtggaa ttcgtttcta ctaaaatatc acgctcggga
    19861 atcccttttt ctaaagcata ttgtttcatt gcaatggctt cagctacttt ttcatcggtt
    19921 ccttttcctc ctgacatgag caagactggc ggattcaaag ttgctttact ttgtgcccaa
    19981 taaaatgcga tcgccttatt gatcctttta gcaagtaggg gagatacgcg ttcaccatca
    20041 atcaagccag cgccgagaac aacgatataa tcttggttat accttggacg gttgaactga
    20101 tacaaaattg aaacagtcat aaaattaaag aaaacgatga gtagataagc aaaaataaac
    20161 ggaatgacag caaatagagc atttgcccat tgtgggagcc aatggactaa tgtatagtcc
    20221 aatatcaaaa agatagtcaa agcgactcct aacaagagag tcaaaaggtt ggccaaagaa
    20281 cgggattctt ttttcatgac tacaacacca ttccaaaata aaaaaatgat caaaatgtag
    20341 ataccgattg ccaaaagaat taatattggt atcccgatca aagccaataa cccaccgata
    20401 aagatatttt gcgtctctat tagtagccac cctagataac caccaaagac aatcaaaaac
    20461 gtattaaaaa gtacgccatt gattaaccta cgtttttcct taaaatagaa aaatgcaaaa
    20521 atactgaaaa atagcaatgg aatcaaaaaa taaaatgtat gataaaaaac ttctaaaacg
    20581 acgaagtaag tgaccatcat cataaaagtc cacgtcccac aataaaagaa tagagcggga
    20641 tcgctttggc gcttgcgcca atagaggatg cctgacattc ctaaaatata gataactaaa
    20701 aatatcaggt ctctagtaag attcattgaa tgaactcctt tcgatttgta aattttttct
    20761 taaaaaagat agcatcattt agtagtaaat atatcgaata tagaaaagtt agttactaaa
    20821 tagtcaatca gttttttcac gacgtttcta ttataacgaa ttatgttgaa aaaagctttt
    20881 atcgaaaaca taaatcttca aattaacaga ttttttagac ctcgatcatg atgaagactt
    20941 ttgtagatac aaataactgt aacattgaaa atgaatgcga aaacgatatt tttttttatt
    21001 ggaaaatatc ggtcaagcaa taaaccagtt gcataataaa atgttaacgg caacaaaatc
    21061 tgtggaagga atgaaccgga tgatttgatg gaaaaaacaa gatagacaag aagtatacat
    21121 agtaaaacgt ggaacaagta ggccagaatg aaaaatcagt ttttatccac tcgcttttct
    21181 atacattcac tgtcacaaat ataatcgaaa gtaattctat tttgagataa agtattttat
    21241 aggagatagt atatgaacaa aaaaagattt aaacgtattg aaagaaaatt tgcattaact
    21301 tatgaccaag ctaagtattt gaaaacacta tttccaaaac aggtagttcg ggataaatat
    21361 agtgatcatt ttatttcttc cttgtatttt gatacaaacg atcatcactt tttaaaagaa
    21421 atcactacag aaaagtccta tcgagaaaaa tttagaatca gaaaatatgg agatcctcag
    21481 gacatatcgc ctatctactt agagataaaa agaaagtttc aaggaatgac agataaacgt
    21541 agaataaaat tagcgcatca tgagctcgat gatatgttgg aaaatatcga attggcagaa
    21601 accacattca gtaatttaga agaagagtat atttttaatg aaatacagtg gttgttcaga
    21661 aggttggcat tggagcccaa agtatatatc tattatttaa gagaagcatt tgtaaataga
    21721 gattcagaag atttgcgaat cacttttgat caaaatatcc attaccgtct aatgaactta
    21781 acgacctggg aacactgtac agaagacaat ctggtagcgc cagaaatcga tgtgctgatg
    21841 gaagtaaaaa cgacaggaga aatacctgaa tggttacagg taggattgga taggatgggt
    21901 aaaaaagaga taaaattttc taaatataaa caagtctata aaagatatat ccaagaggac
    21961 ccgctctatg ttcaatagtg tcttaaaaaa tgagccttct caactcacac tagatgattt
    22021 ctttttatgt atggtggtgt cgattttgct aggcattctg attgcttaca cccatatgta
    22081 tcaaaacaac tgtacaaaaa acttcatgtt tgccttgatt gggttaccta ctttagtaca
    22141 taccgtcatt ttgttgatca acggtaactt aggcacgggg atcgctgttc taggcgcatt
    22201 taacttagtg cgatttcggt ctgctcaagg gaattcaaga gatattttca gtattttttg
    22261 ggcaacgtcg attggtttag ccacagggac gggatatatt gtctatgcac tattcgtgtc
    22321 tagtatagtt gctgctatcc aagtcatcat cttcacgctt ccaatcaaaa ataaacatac
    22381 agtttttaat actgaaaaaa cgatcaagct aaaagtttct aaatccttgt ttctttcgag
    22441 tattattgaa gaaaaaattg cagaatatac agaaaactgc aatttaaaaa aagtaaaaat
    22501 caataaagaa acttgttcct tacactatat agtaaaagta aaaacgagta tgcttgaaaa
    22561 agaattggtt cgaacgatca tggatcatcc agaagtcatt tcgatcactg taggtatgga
    22621 taaatcgaat aaattattgt gataaccaag aaagtactta acaatgaagt aagaccccag
    22681 ttccaaagtt tagcggggac acttcaataa agtactttct tttttgtctt cagcaactac
    22741 tggtaaaact gagagcaagc tttcgtgaga gtacagcgat aaagatcaac ctcccttttt
    22801 atctcgagca atttccttgc aaggctgttt cgttgatctt aaacgagtta gcaacagtca
    22861 aagatgcgat ggccataaaa aaattgcatt aagttggctg gtatctgata gctgggaatc
    22921 taaggaagac tatccattct gtttcttaaa ttccgctagt tgatagatat cagccaattg
    22981 ttccagatga tcaacatcaa agataaccga aagatgtttc cttttattcc catttgcttg
    23041 aagagttttt atcttctctt tggttccttc actcaatgag agtgtgacta atttctccct
    23101 cgttttttct gggaaatgaa aggatacttt gaaaaatccg tcccaaatag aaagccagaa
    23161 gatcggtttt actttctgag agtgggacaa aagtaagaaa cacttttgtc tcattcttta
    23221 aaaacgaata aacggtggga gcagaagcaa ctccttcgga aataagccaa aattcacaaa
    23281 aatttgaaaa gcaattttcg tgaatttctt cttatttctc ggagttaaac acttctgtcc
    23341 caacctcttg tattcacctt ttgatagcca agcttttcca tcattataaa accgcaaatt
    23401 tcaaaaaaaa acgaacaata caaaaagcgt attgctcgtt tttctcttca gtttctcaca
    23461 cctaaacgac ttttgtctcg acctcttttt ggtgactatt catgatgtta aaataaatta
    23521 ctgaaaaaat ctgtgacatg gcgccaacca atgacaaaaa tattagcttt ttcaacagta
    23581 gaagtggtaa tgatgtctgt cgaaggctct tggtttgctt ccaaatagcc taatttgtca
    23641 tcagctaatt ggatcgttgc tgagccaact gtagtgcctt tgttgatagg agcggtcact
    23701 tggtcatccg ttactttctt tttatcaatt gttggagtaa tcgttaattt tccagtatcc
    23761 attccatttt cgacccagac ttttacaggg ctttttagcg caacattgac ggaggtttct
    23821 tttccatctt tcactttgat gtcttttagg cttgggatgc ttgcatttgc ttttccaagt
    23881 tctttttctg accagttgtc ataacaataa tccatcaatc gtgatgtttc attgaatcgg
    23941 gcacttggat tttcttcatg gttatctgca tttaaaacga ctgtgatgat tcgtcggcca
    24001 tctttgttga tcgttccgac aaagcatgca cccgcaaaat cagttgtgcc agtttttaat
    24061 ccatcgacac cttcgtgata gtttacaaaa ccaggtagca tccagttcca attgaccatt
    24121 tcaaccggtg actgtgtgtt ttcgccgaat gtttgatttg tggtactcgt gatttttaag
    24181 acttcaggat aatctaagat gaggtgacga gcaacaatag cgacatcttt cgcagacagt
    24241 tgattttcat ctgtttcacc agtattctca ggtcgattct cacctaggta agaattgttt
    24301 aaacctgatg cattgacgat cgttgcatct ttgatacccc aatcttttag ttgtttcttc
    24361 attagctcaa gaaatttact ttcactgcct gcaacttttt cagctaaagc aaccatggaa
    24421 gcattggctg attggatgaa agctgagtca acaagttctt tcactgtata atcattttct
    24481 ttatgcaaag gaacattcga tagatccggc acgacactta atttttctgc ataatccgaa
    24541 atggctacct tatcttccca agataattta ccctctttga tttgttcaag tacgatatat
    24601 agaccaatga tctttgtgat ggaagcaatt cccatcgttt tttcgccatc ctgatcataa
    24661 aggatttttc cactttctgc atctacggca aaggcagctt tggcatttat tttaaatgcg
    24721 tcgtctgctt gaacagttgc gctaggcaag aaagaagtga tacataagat aagacttgct
    24781 aatagtacaa taaaacgggt attttttttg aacatctttg ttctcctcat ttttaaattg
    24841 attcttacaa cttagaatat tatcagagag aacaaaaggt ttcaatggtt ctgaaaaaaa
    24901 ccttataaag aacaagtctt attaatgaag aatcatagaa agttatcttt tgggttcatt
    24961 gacacgaact ttgactggct gaacttcggg ttcggttagg cgtgatgctg caaaaagggc
    25021 gcctgctgag agtgcgacca aactaaaaat catgaataat tgcataagat cacctcgttc
    25081 ttttttgttt ttttatagta tagcaagaga gtttgaagct actgttaagg aaatgagcat
    25141 ataattgatg aaagtatgaa aaaaatatga agtaggacaa cacaactaaa aatcgatcag
    25201 gtatttctct ggtagggatt cgttttattg tattgataaa atgggatgtg gtagacttta
    25261 tttacagata caaagaaatg atttttttag gaggggtacg tttatggcaa aaaaagaaaa
    25321 agaattatta tcacaattga cttcagaagg taccaaagga ccgtttgtca cgattatgtt
    25381 aaacacccat gttgcgcatc aagacgtaga aaaagatcaa atcaaattca agaattttgc
    25441 aaaggaagcg aaaaagcgct ttgaaaagaa atactctgag catgattggt ctccattcca
    25501 agaaaaaatt actagtttgt tggacgatcc gtctttctgg cgtagtgcca caacgagtgt
    25561 agctgtcata gtatcaccag aacaagtgtt tatccaccgt ttgggtattc ctgtagatga
    25621 ccagtattat gtgagtgatc taccttattt attagcgatc atcaaaaatg gacatttcaa
    25681 ctattcatac tatttattag gattgaatcg tgattcgatg aaattgtacg aagtaacgaa
    25741 taaacaagtc aaagaagtgg ctttgcctga agatgcacca agagatgtcg tcactgcttt
    25801 aggatctgaa ctaacaggag gcgggttgaa ctacgcatca aatggtagtg aaggtacgac
    25861 gttccatgga gtcaatccta aagatgaaga agtcgaaatt gattgggtca attattatca
    25921 agcagtcgat gactatttga aagatacatt tgaaaatcca gagaaatggc ctatcttctt
    25981 atttgcatta ccagaaaatc agacgatgtt caaaaaagtg gcgaagaacc cttattatcg
    26041 ttccgatgtt gcgatctcag cttcacctgc acaactttca ttaaaagaaa ttgaagcagg
    26101 cgcaaaaaaa ctatcgcaag aattagctgt caaagaagca agtgactaca ataagttgct
    26161 tgataaaaag ttcattgatc aactcgttga tattataccg gcagcaaaag aagggaaaat
    26221 atcccattta ttcattgcta catctaattt agcagatggt tttggcgaag atccagaaac
    26281 agaatatgac cgccgtcagg tgctgaatga actagcagat aatgtgatca cgaacggtgg
    26341 tcaggtcttt atccttgaac aaaaagatgc accagacgaa aagagtttag ttgcgatctt
    26401 acgttattga gtgaaaacta ccaaagtaga tgaatggtca ggcgttgata gaagtgtata
    26461 gctccgagag aaggagtttc ctctgtttca gccgttggat tcatcttgag cgagagacaa
    26521 aagctagctt ttgtctctcg ctcattattt ttgtgcttta gtcccattgg ctttgcgagg
    26581 ggaagatact tttctcaaaa aaacgaacct tgcagttgag agggggatag atgcgcatac
    26641 taatttttat cagttaaaaa atgatgtgga tcattagaga attccaaagt tcgttcatcc
    26701 tttttcagat aataaattga aattttttgc aaaagtgaaa atagcaaata gaaattttct
    26761 gaatcttata ggggtgattt cgag
//
LOCUS       DAYYKC010000030        26733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000030 DAYYKC010000000
VERSION     DAYYKC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.030"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..158
                     /locus_tag="ACHBBW_001248"
     CDS             <1..158
                     /locus_tag="ACHBBW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM9152948.1"
                     /translation="DLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAV
                     TGLNIET"
     gene            270..1721
                     /locus_tag="ACHBBW_001249"
     CDS             270..1721
                     /locus_tag="ACHBBW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9152949.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2070..2702
                     /locus_tag="ACHBBW_001250"
     CDS             2070..2702
                     /locus_tag="ACHBBW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9152950.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3290..4729
                     /locus_tag="ACHBBW_001251"
     CDS             3290..4729
                     /locus_tag="ACHBBW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9152951.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5024..5242
                     /locus_tag="ACHBBW_001252"
                     /pseudo
     CDS             5024..5242
                     /locus_tag="ACHBBW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5491..5706
                     /locus_tag="ACHBBW_001253"
     CDS             5491..5706
                     /locus_tag="ACHBBW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152952.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5923..6179)
                     /locus_tag="ACHBBW_001254"
                     /pseudo
     CDS             complement(5923..6179)
                     /locus_tag="ACHBBW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6366..7277)
                     /locus_tag="ACHBBW_001255"
     CDS             complement(6366..7277)
                     /locus_tag="ACHBBW_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9152953.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7296..8300)
                     /locus_tag="ACHBBW_001256"
     CDS             complement(7296..8300)
                     /locus_tag="ACHBBW_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9152954.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8297..10420)
                     /locus_tag="ACHBBW_001257"
     CDS             complement(8297..10420)
                     /locus_tag="ACHBBW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9152955.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10425..12872)
                     /locus_tag="ACHBBW_001258"
     CDS             complement(10425..12872)
                     /locus_tag="ACHBBW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9152956.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12859..13248)
                     /locus_tag="ACHBBW_001259"
     CDS             complement(12859..13248)
                     /locus_tag="ACHBBW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9152957.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13308..13811)
                     /locus_tag="ACHBBW_001260"
     CDS             complement(13308..13811)
                     /locus_tag="ACHBBW_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9152958.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13824..14048)
                     /locus_tag="ACHBBW_001261"
     CDS             complement(13824..14048)
                     /locus_tag="ACHBBW_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152959.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14152..16062)
                     /gene="ltrA"
                     /locus_tag="ACHBBW_001262"
     CDS             complement(14152..16062)
                     /gene="ltrA"
                     /locus_tag="ACHBBW_001262"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9152960.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16808..16945)
                     /locus_tag="ACHBBW_001263"
     CDS             complement(16808..16945)
                     /locus_tag="ACHBBW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9152961.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16942..18126)
                     /gene="mobT"
                     /locus_tag="ACHBBW_001264"
     CDS             complement(16942..18126)
                     /gene="mobT"
                     /locus_tag="ACHBBW_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9152962.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18385..18675)
                     /locus_tag="ACHBBW_001265"
     CDS             complement(18385..18675)
                     /locus_tag="ACHBBW_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152963.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18665..19807)
                     /gene="dcm"
                     /locus_tag="ACHBBW_001266"
                     /pseudo
     CDS             complement(18665..19807)
                     /gene="dcm"
                     /locus_tag="ACHBBW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19883..20047)
                     /locus_tag="ACHBBW_001267"
     CDS             complement(19883..20047)
                     /locus_tag="ACHBBW_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152964.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20040..20714)
                     /locus_tag="ACHBBW_001268"
     CDS             complement(20040..20714)
                     /locus_tag="ACHBBW_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9152965.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20792..22132)
                     /locus_tag="ACHBBW_001269"
     CDS             complement(20792..22132)
                     /locus_tag="ACHBBW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9152966.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22215..22550)
                     /locus_tag="ACHBBW_001270"
     CDS             complement(22215..22550)
                     /locus_tag="ACHBBW_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9152967.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22560..22934)
                     /locus_tag="ACHBBW_001271"
     CDS             complement(22560..22934)
                     /locus_tag="ACHBBW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9152968.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22947..23261)
                     /locus_tag="ACHBBW_001272"
     CDS             complement(22947..23261)
                     /locus_tag="ACHBBW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9152969.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23374..26601)
                     /gene="fss3"
                     /locus_tag="ACHBBW_001273"
     CDS             complement(23374..26601)
                     /gene="fss3"
                     /locus_tag="ACHBBW_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9152970.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADETTHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7763 a         5557 c         4655 g         8758 t
ORIGIN      
        1 aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa attacattag
       61 acaaattgaa aatgttagat tatttatttc atcctagttt tagccaaccc tataatttta
      121 ttttaaaggc agtaacagga ttaaacattg aaacataatc gttactaaat gaaatcaaga
      181 aatatactgt attactcaat tcattgaaaa tatctatcat aatttttaat agaaaacact
      241 attaaagaga aaaaagagag gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag
      301 ttatattaat gtcccaactc cttatagcaa cacctttcca tgtgttggca agcgaaaatt
      361 caacatacaa aattgcaaaa gaaagaaccg agacgactga aatgagtaat tccgatgtaa
      421 cagcagagag tagtgaactt ttgacaactt catcaagtga aagtagtacg acttcaaatg
      481 ccgagtcttc aacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa
      541 caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcgtcaa
      601 gtgaaagtag tacgatttca agtaccgaat cttcaacaac ttcaagtagt acaagtgaaa
      661 taacttcttc gaaagtgaca aaaccaaacc atatagaatc ttcaaaaaag gaagcgccaa
      721 aaacggaagg acaaacaaaa actttttcga caccacagca aatagaatca acagaatctt
      781 tccaatcaca accaatacta tctggtaatg aagaaattca ttttgacaaa aatgaatctg
      841 tagaaaattt tattatgaaa attggtgagt ctgcgagaaa aattggtcag aaatatgatt
      901 tatatgcttc tgttatgatt gcacaagcga tcttagaatc tgccagtggt caaagccagt
      961 tagctcaggc accgaattac aatttatttg ggataaaagg aacttataat ggaaactttg
     1021 tcattatggt aacaaacgaa gatttgggta atggaacgct ttatacgacc caatcaaaat
     1081 ttagagtgta tgaaaactat gaggaaagct ttgaagatta tgccaaatta ttaactaagg
     1141 gaattagtgg aaataaagat ttttatgcag gagcattaaa agctaatagt aaaacatatc
     1201 gagaagcaac taaattcctt acaggacggt atgcaacaga tacacaatac tatttgaagc
     1261 taaatgaatt aattaaaacc tacgatttaa ccaactatga taaagaagtt aaaggtccac
     1321 aaatgagtaa gactggatac caagtgccta ttcataatta ttcaataacc agtttgtttg
     1381 gaaatcgtgg aaatgaattt caccgaggac ttgatatggc agctacacag ggtgagccga
     1441 ttcatgcagt taaagctggt aaggttgtta aagcagagtt tcactactct tggggaaatt
     1501 acgtggtgat acaacacgaa gatggatcaa ccgcgttata tgctcaccag caacaatacc
     1561 tagtaaaaac tggagataat gttttgcaag gacaaataat tggctatgta ggaagtactg
     1621 ggaatagtac tggcccacac cttcatttgg aaatttgcaa agatagtagt ctttctcaga
     1681 gcatgttagt tgatccgaaa accttattat tcggagacta aaaaaggtaa tcaatctgac
     1741 aaagtaacaa gaaaatggtt ggattcttag acctttgatc aggatattga gactggaatt
     1801 gatatctcga ttacgatggg tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt
     1861 attattgccg tctttatgct catattttga gcaggtttca ctagatgaaa acaatttatc
     1921 tatcttgagg atttcgtgta cgtatcgttc agtcttgtat tgtaaattag tcataaaatc
     1981 agactaagaa aaattggaaa tatttttttc tgatttatta aaagttaatt gaaattatat
     2041 ggtacttctt tcttgggagg gtaggtgcag tgaaggaaag atatggtcac cttactgttg
     2101 ttgaggagat tactcataat ggatataaaa aatatcgttg cctatgtgac tgtgggaatg
     2161 aaacgattgt atatcgatca aacttactat caggtagaac caaaagttgt ggatgtggaa
     2221 attataaaaa taggcataaa tataaaaatt tgacaaataa aacttttggt aagttagttg
     2281 caatcaagcc aactaaactg cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt
     2341 gtgggagaaa aaagttagta tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg
     2401 gatgtttgaa atcaaaaaag catcacattg aaaataaaaa attcggatta cttactgcaa
     2461 agtatccttt ggacaataac tgttcatgga aaacaaaatg ggtttgtcat tgtgagtgtg
     2521 gcagtatgtg tgagataagt tatagtaact tattttttgg acatactcaa agttgtggat
     2581 gtttaaaaag gcaagaaaca aggacatgtg ttgaagggac agtaatagag tgtctaacgt
     2641 caaaattacc gaaaaataat acaagtggtg ttaagggagt ttatcgttct aaagagaaat
     2701 agattgcgta tataacttta aaaaaaaaga ggtactattt aggatcgtat gattctatta
     2761 agagtgcagc tcaagctaga aaagatgctg aaaataaatt gtttcaacct ttgattgaca
     2821 aatatcgagg taatcattaa ttattgtgga taataattaa cagtgatata taaacatcta
     2881 taatatatta ttcatactat tgatatttag tataatccta tggaattgaa tatcatagca
     2941 atgtatttga aaagcaaatt ggaaaacaaa taggtgcaac accataaata tgaaaaaatt
     3001 gggaaaaagt agaatggaaa tttgcttacg tctcacgaga tagctcacac tattaatctg
     3061 aagaacaatg aagtcgactt ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag
     3121 ttactataga gtaagttcaa ataaagcatc tccaacttac atggttacat gaggatatca
     3181 aatcgaaatt actcaagctt cctatggcta gagtcgcgag aagttgttgg gcagtagaca
     3241 taggttatat tgttcttagc tacacgatca ttagaaagga aatcctatta tgtatagtta
     3301 tcaagaagat gcaaaagtcc aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt
     3361 taccttcatg tccacattgg atggcagtat tgtcaatatt gcactaccta ccatctcaga
     3421 tgatatgggc ataccaatga atcaagcgga atgggtagtg tccgtttatc tgatgattgt
     3481 ctgttcttgt ttgttgctgt ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt
     3541 tcggattggt acgctgatct ttgtcttggg atcgctgttg tgtgggttca atcattcgct
     3601 aggtatttta ttgttcgcac gggtggtaca agccattggt gctagtatga ctatggctac
     3661 aaatgccgga atcatcacgg aagtctttcc catacatgaa cgtggtcggg cattgggagc
     3721 aataggtgcg tttgtttcat taggctcgat tgctggacca ggtcttggcg gcttgatttt
     3781 gtcccaattt tcttgggcgt acattttttg gatcaatgta ccggtgggaa tcgtaacgat
     3841 gttgattgct gaaaaattct taccaaaaga tatcaccatg agcggccata caatcgatgg
     3901 agggggcttt gctgccattg cggctacgat tatcagcttt tttggagcaa tctttttggg
     3961 gcaagaaagt ggctttttcc atccagtccc ccttgtactg ttcgtaattt cagtgattgc
     4021 ctttgtcagc tttttaaaag ttgaaaagaa agcagcctct ccattgattt ctttcggtat
     4081 ttttaagaac aagattttta ccatgagctt gattacagcc ttgttgattt tttcttccaa
     4141 cttctttgtc aatgtagtga ttccttttta tctgcaaaat gctcgcgggt tgagtcctag
     4201 ctatgctggg ctgttgatga tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag
     4261 cggctacctg accgaccggg ttggcccgga gattttggta ttagtaggct tgggcttatt
     4321 gtcggtaacc caggtcatgt atatgttttt gtcagtggac tctcctttgt ggctatacat
     4381 tgtcgctact gccatcatgg gtgccggcaa tgccttgttc cagtccccca ataatacgat
     4441 ggttatgagc tcggtcacta tcgaaaactt gggactggca ggctcgatga attcctttgc
     4501 ccgaaatttg gggatggtca gcgggatttc gctttctacg acggtcttat atcaagcgat
     4561 gtctgctaag atgggccagc aagtaactaa ctatatcgca aatcggccgg atgtcttttt
     4621 atatggaatg aagattacct ttttaggcag ctttgttttg tgttttgttg cctttttcct
     4681 gactatctgg cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa aaaaccactg
     4741 ttgaagaatt tttggaggcc tgcgtatggg tgagtgggat ttccatggat gatggaagat
     4801 gaagtgaact tctaaaagcc gaaaaactca agtattatct tcaaaatact agtgctcaat
     4861 gagatgcttg atttgatcac aagaagactg cgaaagccgc tggcaaccgc aagctaggta
     4921 tcccaccgat ggactacgtt ttagaggcta ctggttatga atacggagcc aacgctctca
     4981 tctgcataag aatacatcac ccagattatg taaagatttt cgatacttct gtcaaaaaat
     5041 cattaagata gataaatgat ttccgtctag tcaaattggt tcagaatgta gacacacaga
     5101 tggtaagaag tcactcacaa ttcgagaatg gacttgtcct atttgccgta tacatcataa
     5161 tcgaaacgtc aacgatagta tcaatatttt gaccaagggc tatgaataca agcattgcct
     5221 tagaaaacat caagaacctt aggaacaacg gggatagctt ggttaataag agataccgct
     5281 gtttaggctt gcaagtatac tcttttatca agaattccgt ggctttagtc atgagaggct
     5341 taatactatg aaaattttct ttagcatttt tgatttaggt tccttttcta attataataa
     5401 gatgaccact acagtaacct actgaaatat ataaacagcc actgtagtac cattttcaaa
     5461 gtagtctatg agaggagaat cttcattctt atgaacgctg gaattgcgga tatgaattta
     5521 ataaaaaaaa cccttaatga ttttacttcc aattcaatat caaaaggtac tggtatcaat
     5581 ttaagcacaa taaaaaagct gaaaagtggt gaaagatcag tggaaaagct gaatctttta
     5641 gatgctataa aaataacgga atttgctatg aaaaatggaa aagctgagat tgaaatatgg
     5701 agataattat cgtctagtga attacttgcc acttagctga atttaacgat ccttggtgct
     5761 tgaaggggga gattcttggg aatagagcat acttgcaagc ctatgtcttt actaacaacg
     5821 atatctctta ttaaccaagc tatccccgta gttctaaggc ttttactttt atctaagcaa
     5881 taatcgtatt caaaggaaat ttttgaccct tagaaagtct cattttatca gacgtgagct
     5941 taggcattgc tgaattttgt taattcggtt ttaggttgtg ctttgagcat aatatgaaag
     6001 tattctttat cacgatttca ttcatctaag gtaatgtgat gtgattctga aattctttca
     6061 aaggcagttt ttgcataagc tgacattaca tcatcaatga catgtttaca atgtttcact
     6121 actaaaataa gggggtaacc aagaagaaaa actgaatgat tatttgtatc taattccatg
     6181 gtatatcaac catttcctat atagactgat tatgacatgg attaaaataa aaaggcacta
     6241 tgtcttaatt gtcggttttc gagttaccga atggcattca tcacccacct atagaggatg
     6301 ggcgacttct gcctgaaata agttacacta cttataactt ctaataaaat tagattgagt
     6361 cattattatt taacatcaac gattttccag tttccatttt tttgtaatac taatttgtac
     6421 tgaaagtagc tagtggtttt tgatacatta tcaaggtatt ttatctttgt actcactaga
     6481 aaattttttc cgttttgtag ataaactgga ttctctaact ccgaaaactt taagtcaata
     6541 ttgattggtc gcatcgcacc attttcaaca taatactcca attctttttc agaagctgat
     6601 ggataaagtt taaaaaatgt ttctaaaaaa tcggtgattt cttccgttgt tttggcatca
     6661 atttctgaat cgttttctac ttgttttgct tcatattttg ccttgtctgg ctttccaatc
     6721 attgttggca aactagttac taaatgattc ctgttttcat cttcaaagat tgtcactcga
     6781 tatgcagaag aaattgattg cgtctttttt ccctctgtaa tatgttgctc cactaaaaat
     6841 gaaacgacaa attcttccga gtgtttttca acatctagaa tttttacaga ttgaacttcg
     6901 gaactcgtgg gaatatctgc tcgaaccata tcttggatta aagcgagacc gttttctgta
     6961 aggtactgtt cgagattact cattctcttt tcaattgctt ctttgttatt tttccatgaa
     7021 aaatattctt tcgcaaaatc ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc
     7081 tcttttataa ccactttttc atgagttgta tgttgatcaa tggcggtgaa atttttataa
     7141 acgccaaaag caagactaca agacaataaa agccaaaggg caagcaccat ttttcgatgc
     7201 ttacccacgg atacaactcg ttcttttttc ttttttgggc tcttttcctt ttgattccgt
     7261 tcaattttta tcttcattct ttttcctctt ttctcttatt tgtatcggcc agctccgata
     7321 agatgctgcc gccaatagtt ttccgttaga ttcgtccacc ccactggatc tcctgcatga
     7381 aacatgcgat tctcccctgc gtaaatgccc acatgggtca cataagtacc ggcatcatag
     7441 gttgaatgaa agaaaactaa atcacctggt ttggcatctt ttagatcaat atgagtcatg
     7501 gcatcatatt gatcctgagc cacccgagga agtttcaggt caactttggc ataacaccat
     7561 tgggtcaagc cactacaatc aaaccccgtt tctggacttg agccaccaaa cacataggcc
     7621 gttccttcat acttaaatgc ctcatcaaaa atttcttgga ctattttgtc atcaaactga
     7681 gttgtagtta ggtattgctt cactaataaa cagtaaaaca tattgccata attataccgc
     7741 cacccaccat tttccttaat cgcgattggg ttggggtaat cgactttttg accacctgat
     7801 ttttcctttg aaaaagattc agctaaagca aaactatgct tcttcccgtt tttcccgaca
     7861 tgatctagat acccgccacc atagttatag gcctgaataa tactatcttg atcacaccct
     7921 agttctttgg aacgcttaac caaagacgca aaatagagac agccttgttt gatggatgcc
     7981 tcggtactta atgaattagg tggtaatccg gctgactcag aactttgcat aacgtcttcc
     8041 attgtgccac cagattccac ttgaataatc gccaaaagaa ttggaatata ctctgttata
     8101 tccgattctt tggcatactt ttccaccatt gcacggtgtt tcaatacttc ttctgagaca
     8161 ctagttccgc caggagaagt gccagaacta ccatttccct cactgtcttc accaaacaac
     8221 aaacccacac acatcaaaat acccataaaa gagatggtca gaaaagttaa tgaaataaca
     8281 cccactttct tcaagttcat tttttctcac ctgaccttga ttttggacga tggttcattc
     8341 ttttttgtac tttttgattg gtctgctttg gcagagtgga cctctgtttc acttgttgga
     8401 aagaattttt cttacttgct gattggcttt tcccttttga aatgatttct tgactagtta
     8461 gttttcgttt gatttcggga ttcagatttg ctctcacaat ccgctttggt gcttcttttt
     8521 tcagcatcat atctttctga ggttgagctg ctttatctat tggaatattc gcccttcttt
     8581 ttccctgacg atttgttgct tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc
     8641 ttcgttgtcg ttgtttttct cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc
     8701 gcttgaattc ttctggtgct tttttcgact tttcgattcc tttatggaga ttgtatttca
     8761 aattcgttgg cgtattcctt acctgatcct ttacttgttt agtcttatct ttcaccaaag
     8821 cttgggtatc caaaactttg ccgatttttc tcccagccaa attcattcgg gattgtttct
     8881 tattatttga aactggattc tccttagtta agtcagatag tatttcgtga tcgttcggta
     8941 atcgtgaatt ctttcgtaga gaagaaccgg atggcttaaa tttggatttt gattgttcct
     9001 ttgccaactt atttccgact gctgcaccac caccaaaagc caaagctcgt cccacatttc
     9061 gattgagttg ccgcaatttt cgattcatta acatttgcgg ttttcgcatg acgcgtcttc
     9121 ccattgactg gctatcatta ctctggaggt tgaacatact cataatgtcg cccaatttga
     9181 aatagatacc ggcaaaggtc acaatttgca gaaaagcaac catgaaaaat ggataggttg
     9241 ccgaaatatt gaaaaacatc gtggagatgc taaatgcagt cgtaatcacc aaagtaacac
     9301 ccgctcgcat cataatcgta ttgaataaac gaataatcgc cttctttcct aaactttcat
     9361 aagtcggcaa catggacaat aagaaactaa ttggcagaaa catcgcaaag ataatgaaga
     9421 gaatttgcga gaacaacata atccctgtta gaagaaaaac aaagaaggaa atgccaatat
     9481 tgaacaatac caaaaagacc acggtcccta aacggttcat ggtcttggta atactgagat
     9541 tcgcattgtc attgtcttca atctctgcct tgaccgcttc ttcccgatct ttgcctttgt
     9601 tttcatctgg actcacagat agaatcttgt tgacccggtc ttcaccaatt tcttctatgt
     9661 tggaatcatc aaattgcagt agtaaccaag gttgttggac ttgaatcgaa aacaaactat
     9721 cccgaatcaa atccacacta tcttttcctt ggctttctga attgggtaca acgatctcgg
     9781 ttccaagggt aagggccgct tcactaacat ctgagctaaa gtcattgatt tttgtgatat
     9841 aggtaggcgc ataagcaatg aacgaacccg atagtagaaa aatcatcaca aaattaatca
     9901 cggctcgaac tgctttcgtg gtttctcgtt ttaataatcc ggtatacgcc acatacaccc
     9961 ccagagctaa aatcatcaat aatagaaacc caaaataaaa tccggaggtc tggagtccat
    10021 tttcagtaat accagccaat gtttggatat tctttccaat actttcagcc gtatccgaaa
    10081 tgaaatctaa tttgtaagcc tcttgaacca cataacccgt ggcgttcgac aaatacaaac
    10141 tcaccgtcca aatgaaatta gtaatggcat aaagaccata catcacgctt ttccctaagc
    10201 catcacccca attccaaggc aaccaatccc aagagctatc cacaaagaaa tctagttgat
    10261 agttgttcag tgaatatttg gaatatagat ttcctgcttc aactgtatca tcaactagcc
    10321 ctgtcgcctc ggctaccgta ccaactaaac tcaatagaac tatcagagtg agtatgctaa
    10381 taaaaacgat tcccacaatc cgaaaaagtt tctttttcat cccctcactc cattctctct
    10441 tgaacaggtg gacgggtatc aaaggcatgg aagagttctt caaagactgg atgtacctga
    10501 accacgccta cccgaccata caaatcctgc atcaagcatt ggccattttc caaatctcgt
    10561 aggcgttttt gattgctttc atcttctcga tccaagccaa agaattccaa ggttttcttg
    10621 atttcatgaa tatcggtact gcgaaacgca aacttcaacc cgatattatt cttcaagcgt
    10681 tcatccagta aatcatcact attttgcgtg acaaagtaaa ccccggcatt catagcccgc
    10741 ccagcacgaa cgagcttatt cgataatgtt ttcccttgcg ctacttgaag aaaactccac
    10801 gcctcatcta agtctacaat tttgaagatt tctcgattac tatggataaa gtccaaggca
    10861 aaagtggaaa tgacgattag catggaaaca ctaagcaatt ccatggtggt atattcttca
    10921 aaagtggttt ctgcatctgg taaaaccaaa tccgctactt gaatgatatt taactgccta
    10981 tctaaactaa tcgactgggt aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa
    11041 tcggtaaagg actcgatgtg atccgcgata ttttccgcaa ctggtgagcc ttcttttcga
    11101 agctcattaa ttacttgtaa taatccccgt tgattgcttt gggtaaccgt ccgaatggcc
    11161 ttacgcaaca ctggaaactt ctccccatct cgactagaaa ttcctgttaa aaaggtcaga
    11221 atatcaattg ctagactttc agaatccttc tttttctcca taatcacata aggatcaagt
    11281 aatccttgat tttttggctt actagttaag ttgagaatat tgatttcatc cgcaatttct
    11341 ggcaaggttt ctttccagcc accccgttcc ccttttggat cgacaatcac agcttgtcca
    11401 ccaaaaagga cggcgtaata aactaagaga ttgttactaa aggactttcc gccaccgagt
    11461 gaaccgagga atgccgctgc tagcgcattg gtaactgaat ccttgacccc ttgtgaggcc
    11521 aatgccggtt tcaggtaaac attgcgcccc gtatctacgt tataaccaaa gtaaatgcct
    11581 tccaactccc ctaacatctg agtggcgcca aaaccaagtc cggcgagaaa atcagacgta
    11641 acatactgga tataatcatt catgtatcgc ttactagaag gtaagaattc ttcatgtagc
    11701 cccatcatat cgccaaaagg ccggactagt tttacattgg tatcatcata aaaatctagc
    11761 acttcatcac accggcgctt tagttcatct actgattcgg cacttactcg aaccacatag
    11821 ctcagtttgt acatggattc tttggactga tccaaggtag tttccaattc atctacggaa
    11881 tccaatgcat ctaagacatt ggaatttgtt tcattgtcag attgataggc atggttgtcc
    11941 aagtctttta attccttttt cttattgcga acggtagcaa gtgccttttt gttcgtcaca
    12001 atctccacat tcatactagt gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa
    12061 agttctgagg agggaaactc catttccccc acaattgtat taattgttaa gtacgctaca
    12121 tatgattcat gattctcatg ttccatgcat aagtaccggg gcttttcttc aatcaaacaa
    12181 cgactgggac gtaataagtc gtaacgtttc accaaagttt ccgacttcag ctttttcttt
    12241 ggtagctgaa attcatattc ttcaaatggt gtaccctttt cgccatagat atgttcaatc
    12301 aaatacatca gatcactcgg tgtgactcgt ctgactttaa atcttcgagc caatttactt
    12361 tccattagtt tttcaagctt ggtatatcgg cgaatttctt cattggataa ggaaacaaaa
    12421 tcgcccatca aatgatgatt cactccatag acaaattctt gaaagccgga gaagaaagat
    12481 ttcttgagat tctttagatt gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa
    12541 aagcgataat caatctgact atcgccaatc atggatacta atgcctctgt ctgtaaatca
    12601 attcgttgtt tcgctacttc tgttaatcgc ccggtaattt ccttttttga tcgttcttgc
    12661 gtggcacgaa tactactttc tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt
    12721 tgcgcaatca attgacgaaa gttatcgtgg acttgatatt tctgttcagg tgacaaaaag
    12781 gagtaattgt aaggtattaa ctcatagtag gcaaaacatt ctccctcttg attaaagaca
    12841 agattatttt caatatactt aatcgggtac atagttcaca ctccttacca gagtaatggg
    12901 ttcgtcccat cgtttcttct gttctttgac tttctttcct gcgaaagtga ccttaaatcg
    12961 taagtgatag ctaataaaag atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt
    13021 ttgcgacatg aaccaagtaa ctgccaccgg tatacctaag tattttaaaa aggccccctc
    13081 tatcatagat agtggaggca catgggcaaa tacgatgaca aacagcaagg ataaaacaaa
    13141 ccaagccatc tggttaaaag tcacaggaaa aggcagttga aagtcattaa ttgcataaat
    13201 gaccttttca actgcccaaa tactggtgta gctttttatt ttcttcatca aatcattcct
    13261 ttaaaaagaa ggatagttta gaaatcaact acccttcaat aattttgtta atatggatac
    13321 tcaaaaacac ctcgactcgt taccaaataa ttgcctccaa tctccaagtc tctaccacac
    13381 gcatcaaaat caatatacgt cttcaagtgt tctggtactt ctccaaaatc accagcttct
    13441 acaatcaaga aatatgctac atcactcata tcatcgcaat ctggataata gataatatca
    13501 tccacatgct ctaccatctc ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca
    13561 acttcaccaa ctggcgttcc ttcaagttcc tccgctaagc cacacaaccg attaatttcc
    13621 tcaattggcg tatactcatc aatctcaaag ggaagttcat aatcatgaat tgcatattcc
    13681 tcgtattcgt catttaaccc aattcgttct ttcacttcat caaaatcgac aggtggggta
    13741 aaccacgccc ccaccaattc accttcgtta tatttcccga ggttcgcaat ataaacacgc
    13801 atctgttcca tcgtgcttcc cccttacgca ccgacaattc ggttaaacaa actgagtaag
    13861 acatccttca ccccagaagc attaaagacc aatccgaccg caatcaaagc aatcaccaaa
    13921 aagccaatca gtttggaaaa ctcccgctta aatcctaaaa acaaaccaat gaccacaatt
    13981 gccagtaaga ctaaggattg ggcattggtt aaaaaccatt gatatatgga taggtaagtt
    14041 cctgatatta taaatatctc aaacttttcc ccccattcac acggagcgtg cgactttcac
    14101 cgcactccgc gttccatcgt gtttatattg aaaagtattt atgtagtaga cctatatcaa
    14161 gtttaattct tgattgccag ctaatactct atatttattt agtcttatga gctggacttt
    14221 atttagcttc aatagagcta tatatttttg tattgtttct tctgttttag cgtgtattag
    14281 acggtgtacc gcctctttta ccaaaataag atttttataa tcgtcatttc caccagctcc
    14341 agttggtaca atatgatgac aatgtatttc attagcttgt tgaaattctt ccagagttat
    14401 agcacatttt ccatgttgag cagaaaatag cgaaattcga ttgtctgcgt attctattgt
    14461 gcgattttcc aaagataatt tcattaactg gtacatcagt aatttatcaa cacttagatt
    14521 ggtgtggata aattttcttc cctctagagt gtagcaattc gctaaataca cctttgccat
    14581 tggttttctg tgttggatat atccaacagg gaaaataggt tcgccctcgc cagcaacata
    14641 ccgaagcatt ttagatttac catatcttgt tgcctccttt gtcgtaagtt gtctgcctgt
    14701 ttttctcaat ctgccccttt tgtttgtacc taatctgtta gtgaagatag ccattacagc
    14761 tctattcagt ttcatacaat ccagatttac attcgttgca atctgataat aattttgcat
    14821 acctaatacc attgaattat aaagtcttgt ttcttctaat tccgttttcc cttttgaggg
    14881 agaagcaatt ttctttgcct gtgataccag cttgtctttt tggtttttga attgtttatc
    14941 acttatatgt gatttaacaa ctaacttctt tcttctcggt atcattttaa tcttgaaacc
    15001 aagaaagtcg gagtatcttt ttctaacatt tacaattctt gttttttctt gtgatacttc
    15061 caattttaat cgttgttcaa tccattgagt gattgctatt ttggcttttt cagcactttc
    15121 cttgtagcgg cagaaaactc taaaatcatc tgcataacga accatataca tttcctttaa
    15181 ctttgttttc ttcatttctt tgtaagcgtt gcttttacat gggctaccgc ttttattgat
    15241 atgaaccaca tagttttttg taacaggatt ttcctgccat tggctttcta cccaatggtc
    15301 taactcatta agaacaatgt ttgctagtag tggggatatt attccccctt gcggagttcc
    15361 tttctctgga tatgttattg taccgttagt cattttgatt ggagctttta atatcctttt
    15421 taaaatgaat atcaagtgct tgtcccgtat tcccatagcc catatctgtt ttatcagctt
    15481 ggaatggttc acgttgtcaa agaaaccctt aatatcaaat tcaatcacat aatgtagctg
    15541 gctgatttgt aggcggttat aggttgcttt tattgcattt tcaactgacc tattaggtct
    15601 aaaaccatag ctgttttcac taaatttagc ttcgcagatt ggttctaata cttgttttat
    15661 acattgttgt ataagcctgt cccacataca aggaatacct agaggtctgg ttttaccatt
    15721 tggttttggt atttcttttc gcctcactgg cttaggtcga tacccatgtg gactgcctgc
    15781 aacaatgtat cttacttttt tgacaactgt ttctgctggc aatttgccaa tatctttaat
    15841 tattgtaccg tctgttcctg ctgttttact gcctgcattt gctttgatat ttcgatatgc
    15901 cagtaatata ttttctctcg ataggataat atccataaga tgagtgaaaa tatctccatt
    15961 cttacttttg gcatataaat catcaaaggt gttttgcatt tcgtaatatt cagcgtgtct
    16021 taaatcttct acacacaatg tttcattttt atttttaggc ataaggcttc actcctttac
    16081 ttgaaattga gtgagctttt ttatcttact cgtaatcctt attgaaacaa cataatactt
    16141 ttctaatata tacacgactt gagggcattc ctccattctc attacaagaa tttcttaggt
    16201 tcgccctctg cttttcagca ttagttcaac tgcttattgt tcttcgtcag tttattctct
    16261 tgatgagaac ccaatacctt tcctcgttcc gataatctta tctatacata tatatcctta
    16321 ggtcactagc tataagcctg ctggcttgat accgcctgta acgttatatg gcatttcata
    16381 acctatattc ttactttgcc acaccagcta cgatttctcg tacctttgca tttctacaaa
    16441 gttcaaattt agacccgtac attcgctgtt tcgtcagttc cttatagaac attctaacca
    16501 tagatatttt atagaccccc gacctgttgg atacgctacc cacctctgga tagttttcga
    16561 tagaagatat atgttcatca actattacgg tatctttcag ccgtctttac cgagcttcaa
    16621 acattctgag tttcctcata caatgcttgt cggaggctca gggcaggcgt ttcagagcgt
    16681 tacttcatca ttccacctgt cagattatca gttctcctaa taaccaaact ttttcgttct
    16741 tttattagtg ccttagcttt tcactaagga acgagtcgca cataaatttt gtccgaaatt
    16801 catcttgtca ctttcctttc ttttccattg ctcgatccgc tgctgcactt gcattcatca
    16861 agcacatcac agtaacacca agcatactgc caccaaatac caaaaaaata tcttttacaa
    16921 ttcccatgac gattcctctt ttcatataat caaattttca attgccatac tttgttgctc
    16981 caaaatcttt ttatgtcggt ctgataagcg tgcattctgt accatatcct ggatataggt
    17041 tgtccgattc aatcgatcca actccttagc cattttccac gtaggggcga cttgtcggcc
    17101 tagccaattc aaggttcgtt caatggtata cggttttggc tcggaagtta attgaatctc
    17161 tttccgattc cgtccaataa aacgccccca ttgttcattc aagggccagt tggttcgcct
    17221 tttaccttct actttatccg caaatcgcaa gtaacgattg ataatggaaa acgtggtgct
    17281 ctcaatgcta cgacctttta acaaatcttg aatcgcatga aaagcacggt catttttcag
    17341 tcgaatctca aaacgatttt tcgtctcagt ttggtctaat ggaatcccta acttgatata
    17401 ttgttcatag tctttttcat acaaacagaa ataaacctca ctcttgagac tgccgatata
    17461 taaggtattg cccatgccgt ccttttcatc agcctttaaa agttccccgg aacgataact
    17521 tttaaaggta cgaaacaagg agatacattc ttccttctga cattttttcg ttagctcagg
    17581 tatatccaat agccccactc gatcattgat tgccaagtcc aagcgtttca tcacgccacc
    17641 ggcctttaga caatcctcga aaaagtcgta ccagctacgc ttttgagcga gtaaaaaagt
    17701 ttcaaattgt cggcaacctc gcccctttaa ttctaaaagc acacctttat cttcttcatg
    17761 agacaacatc accacaatat cacccatgac atactgttct tgataggagt aaaaggcata
    17821 atcttcatgg agcatatact caaatttcaa atggagaatt tcttgaataa tttttcgtgc
    17881 atccgttgtg ggaaagcgaa tccttacata gtcaaacagt aacgtcaaag gcaaatcggg
    17941 attaaagctt tccagttggt caaaaatttt cctcatggtt tttatagatg cggttacgcc
    18001 accattttct atctgattta agtacggccg actaagtcca caagccgtag ccaatttatt
    18061 ttgtgaaacg ccgtattcgt tgcgtcggtc ttttaatagc cgataatcta aattccgttg
    18121 tgccaagttc tcacctccaa attcaaggtg ttaacttttt caaatgagtt aacacctata
    18181 caaattactt actatctttg atacaacaag cattcttaat ggttgcttac ttattttttg
    18241 tgtttttgta cccccctgtt agatacgggg gtgagacatc atggtcggct aacgccgtcc
    18301 atgaacaggc acagcaccac ggaggatttc tgcttcgcga atcctccgcg gtcagcgcct
    18361 aacagtcagt tgtgttggtt taggttactc atctgtaata ccatttactt ccaactctgc
    18421 tttcatttct aacaagtatt gacgaatatc tggttctttt gcttggtccg caatccgctc
    18481 aaattctgca attacttgat ttatttcaat tcgagcatcc gtctcctcat tcattaaata
    18541 tggatcgcca gcgactttga caccattctt gcgaatatca aattttaacc gtcgcaattg
    18601 ttcttctaat tctgggtctg actgaatctt tccaatcacc caataaattt ctccaatcaa
    18661 ctcatcataa accatgtaca tccaactctt ttaatcttct ggcaatatca aaaatgaccg
    18721 gaactgttac agagtttccg gcttgcttat acaactgact atctgaattg acttgagaat
    18781 ctcgatcaaa agcccaatcg ggaaagccct gaagtcgcca acactctcta ggtgttagct
    18841 tccgaatttg atactgatcc gtgacgatcg cttgttggtc tcctgttagt aaggtatgaa
    18901 cactctgttt tcccagacga cctcggcgac ttgtggaatt aggaaaagcc agattcacac
    18961 tgtcttctgg cttcacctct tgatattctg tttcattttt tcgaatcatg atttttgtac
    19021 caccttgttt cgttgacaag gtgggggcgg ttccagtaac ttcataaact cgattacctt
    19081 gttcaaactt tcccggtaat agtcctgcta cagagattcc atcccttgct ggcagtgcga
    19141 ttttgattcc ttcatcacct gttaaggttg gcgctacgcc atctgaacca tagacttgtc
    19201 cacccattcc tcgattggaa ggattgatat ttaccaaatt tctgatgttt tgatcagatg
    19261 ttgaagacgg tccaatgata ggaaatactt gttcggtaca tgcgtctcga gaatgtccga
    19321 taaggaatat ccgctcccgg ctttggggaa tgtaggcctt tgagttaagc acctaccact
    19381 ccacgtcata ccccaatcca tccagcgttc ggaggatcgt ctcgaacgta gcccctcctt
    19441 cgtgattgag caatcctcgg acgttctcaa ggaataaaag cttaggtcgg agaagagtgg
    19501 tgaaccgagc gatttcaaag aataaagtac ctcgagtatc ggaaaatctt tgccgctttc
    19561 ccgcaattga aaaagcttgg cacggaaatc cgccgcaaag gatgtccact ggtccgaggg
    19621 attgaataaa ctcatctgat acacttgtga tgtcatgcat ttccacctcc ttacttgtat
    19681 catgaattgc tttataacta cgtctagcaa atttatcaat ttcacaaaaa ccgatacaat
    19741 gatggctcgc ctgttccata cctaaacgaa agccaccaat accggcgaat aaatctaaaa
    19801 atctcataca ttacctcatt cattgcaaag aaaaaagcca agaacatttt gctcttggct
    19861 catacattat taatttgtcc tactattttt tatttaatcc aactaatttc cgttgttttt
    19921 cattggcctg atcggctaca tatttaatca ccgttttgaa ctcttttgca gtagcatttt
    19981 ccgataaatc cgcaatgctc ttattgtatt tctttacaat tttcgttact tgttctttct
    20041 cagtcatgta attgaacctc caaatctttc ctactatacc ctagtggtaa aaataattct
    20101 agattatccg tagcataatg aacttcggaa atattattcg tataatcttt tattatgata
    20161 tgaagcaata catctggatg tttactcttt acttttgcaa tgttctctct tgatttgaaa
    20221 aaagcgttaa taaatgcctt tttcggcaca tgacgtccct ctgcaatttc acgttcttgt
    20281 gtaaatttcc aagcgatttt agggtcttga taaacataga aaatcgtcac tcgaaaatgt
    20341 tttttcaacg cccgcgagat atttttttca gcacttaata ttgcaaaagt tgcatccaga
    20401 ataaaagaat agccatgatc taagacatat ctaaatgcct gctctaccaa ccaagaagat
    20461 gctttttgaa agagactaga gttttttcca ttataatcag gaaactgggt gcgaaagtaa
    20521 tccgcatcaa taatcacatg gttatcataa ttctcagcta atccaatggc aacctctgat
    20581 tttccagccc caggactccc tgccataaaa actgcgtctc gtccaacttc gggggatttc
    20641 cctttgataa tataagaaat aaacttatcc ttatttgctt ttgcatactc taaataagaa
    20701 tcattacttt ccatattaat tatccccttt cttcctacta tataaaagta ttatacgtta
    20761 cttgaagtat aaagtcagta ttgaagatca ctcaatcatt catcttttct aatttactaa
    20821 ttgaatctaa gaaatcatac ccttttggta caagtggcgt ataaaactca ctaatcacac
    20881 tgccacctgt atccacataa cctcgacctt tgatgtgctt cataaagaag tttttttcga
    20941 cctccccaaa catcatggaa tatccgagtt cactcatacg acccaaagcc acgcgaaagt
    21001 tgaattgatc gcggattccg tctcctaaat atttcgcatc cggcctttgg caagccaaaa
    21061 tcagaaagaa accagattga cgacccaaca tcacgatttg tttgagctta ttcaaaatca
    21121 ccgcactttc tttcgtcgtt aacatctcca tataagccac gtattcatca aagattaaaa
    21181 agttgggtgg aagtccaaga taggcataat tctctcctgt tttgtagtta gacatttctt
    21241 tcattgcctt gctacgagcc atcatacgtt catagaaatc ttccacacaa gcagaaattt
    21301 cttccttttg tgaataaaca tgaggcatca ccgtacctaa atcagctaaa tctgcatttt
    21361 tgggatcgag gataaacaat tctgcatctg acttcaatag tgcttcgata atggtgagga
    21421 ggaaataggt cttccctcca cccgtgccac cagcgattaa catatggggt aaagaatcat
    21481 aagcccatac ttgatttttc atcaatcgca gagtcccatt ttctgccact acttcgtcta
    21541 tcccaattcg attggcgatc atatcataca gtaaagtata ctccacatag gagtctttga
    21601 gttccttttc tactaactca caatacagtc cgctttctaa cttcttttcc aattttaaaa
    21661 gctgatcttg gtatttccca agtgaaattt gaacgcgaat agacagtaat ccatctttca
    21721 ttcgataaca gattttgggg aagtaactga tagtctctct ggttcggcta ctattcaaat
    21781 ctttgaaaaa gccttcactt tgagtttgtt tcacttcata ccaatggttc tctagaatca
    21841 tccgagctag cttttgtcga tgccacaagc ttcgccaatg atcaagaaaa aagtgaatat
    21901 aaagcaatat catagctcct gaaaaagcga gacttccaag cagacatact aaatctttta
    21961 tagaccaaac gatttcaata gtagaaagtg atagcttaga aatatcaaat tgtctaaact
    22021 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
    22081 agtgataaac caagtgctga tctcctgcgc gtatgcgatg tcctttgtac attttgatgg
    22141 tttccacctc caatcgaaaa agcccaatag gatttcatta ggcaatttca aaaagttatg
    22201 ctagtcattt actgctacga ggaggggagt tgtttcgttt caaccaattg atttaaaaag
    22261 ccttcaatag catccggcat ctccacacca atttcttcca aaatggattc tgcctgccga
    22321 cataagtttt catttacttc gacaactata aagaaagact cttcttctcc taaccagtct
    22381 agtacatcag agtcttcttc actgtcgcta ttttctccaa gtaaaccttg atgtataatc
    22441 agaaacattt tttcatcaga ggttaatgct ttaacgagtt gttcctcagt cagctcgcct
    22501 tttccatatt gatcgagaca atctaaaata tgttcaacat taatttccat aatcttcacc
    22561 tatttctttc tttcattttg gttcgtagga tttccttgat tcggctgccg cttcaagact
    22621 aaatcatccg ccttaatgaa ccaattaacg gtagttcctt ctcgaaaaac ataattggca
    22681 acggtatcca cgattggatt gaccaactct acctctgcat cataagcaaa ctcgcgtaag
    22741 ggtacattag ctgggatact aacttgaatc attcgccctt gtgcagagga ttttaaatta
    22801 taggttcgtt cttttacctc attaccaatt gttccatcct cattttgctt ccgtacttct
    22861 cggcgtaatg cggaaaaacg caacttccca aatgttaaac ctgaatctac gacaatacct
    22921 tctgttaatc tcatcttctt ttcctcctat gctttcacta aatcatcaac atacaacacg
    22981 taatcagtaa acccacgatt ctcaatctca tagcctttgg caaccaaatg gggattcacg
    23041 gcttttagcg gttggtcttg atcaaaatcc ttttcaccgg cttccgcagc tactaccacc
    23101 tcaatatcat cggcccgttg cttactggaa tacaaatgat aactgcgacc aatcaccgta
    23161 gtattccgtc catatccttc tgttataatt tctccttcac cggcataact taactttcca
    23221 aaggtttcag ccatatctgg cacgacaaat ttcaattcca tcttcttttc ctcttttcat
    23281 tgttagtttt ggatacaaaa atagcgcgac tactttttta ggaggcggtc gcgcacgttg
    23341 gttcttagga tttacttgta tcatctcatc accttagttc cggcgattcc agaaataata
    23401 gcccgccgtc gcaaaaatca aagtaaaacc aatgaacagt aaaacattgg aatttttctc
    23461 cccggtttga gggaaggtct tcactgtggg actagattct tgagagccac ttggaacagt
    23521 tggtgtttcc ggttgtttcg gcgtacttag tatggttgga acttcttttg gtgttaaggt
    23581 ttgagatttt tctttcaaat cttcattgtg ttttccattg atattcccat ctttatcgta
    23641 attgatttcc gcaaaagtaa acttggttcc ttctggatat tttccggtat ctactttttt
    23701 cgcaagcaca gttttggtga attctttatc atttacttca taatcaatct tgccagattt
    23761 ccaaatttct ttgtttgtac catccggtaa taaagcatac agaattgttt cgaaagcttc
    23821 ttttgagcca tccagtacat catgagtaat cgacacatca tcaaacatat ccaccacatc
    23881 accatgagta aaggtctgag aaccgttttc taggtgggcc tttgtttgga tagaaacatg
    23941 gtgttccact gtacaactga cagtttgggc ttggttattc aagctaacat ccttcgctac
    24001 tggcttatcg cctgcttcaa aagcttcctt attttcatac acatagttga ataagacaat
    24061 agttttattt aaaacttgtt cggcggttaa tttatgctga attttccaag ttcctgtttt
    24121 ctctttgttc gccaacgttg cgcttgtttc tgcaatcacc actggtttgg ctttttcatc
    24181 tttttcttgg gcagcttttg tcgcatccac atcaatggct tgcgctacca cataccagtc
    24241 ttctttgatt tcatgtagat tgtaactcaa tttatcaact agctcggtag aatccagaga
    24301 agttaattct ttgtttccgt ctttccaagt cgcaagagaa gttaaactat cttcttcctc
    24361 aggcaaatca tagagcatta aacggttcaa actaacagaa aatgctttgt tcgtcagctt
    24421 ctcgtaagga acggtcaccg ttttaattgg ttgtttttgg tcttgttcgg taatggtaaa
    24481 gacatactca ctcttcacaa agtcttcttt gttttcctta aaagtagaac ggatttctaa
    24541 tggcgtaatc ttttgaaaac cttctggggc ttctacttct tcaagtaaat aatccccata
    24601 aggaagattt tcaaacttgc cataaccatc aaaccctaat tgctcgttat aagctgtggt
    24661 cgcttcatct tcggcacctg tgatttcatt ggtcccttcc aatggcgaca ctttaaaggt
    24721 cagatcattg aagccagttt cggtagtgcc cgcagctgat ccagcaaatt taaagaaatc
    24781 aaagccaaag cgaatcactt gttcttttgc cgtaacattt cgggtaatca cggcattttt
    24841 ttcgctatca tccacctttt tgatggatac aggatacttt gtttcatcaa ggctatatcc
    24901 ttcgggcgct tttgtttctt gccaataata ctcgttgata gctaaatgtt taacggcaac
    24961 ttggttcttt tcgtctaaag tcaaagtgac cgtttcatca gaacctttcg ttcccttcgc
    25021 taactctggt ttaaaagcct cactccattt aacagctttt ccatccttag cagtaaacaa
    25081 ggtgtattca gcaccctcaa agactgcctt accttgtgct ttatccccag tatctttgtc
    25141 ttctttcgtc aaagtggttt cgccagtgac ttcttgattt tgccctttta cgttactggt
    25201 aacaggggct acggtttgat tggcatactt caattcgact tttacaggtt tgaacgtatt
    25261 cacgaaccca tgactagctt tagtttcggt cacataatag gttcccaatt ccaacgcatt
    25321 agcaatctct tttggtgttt ccgcatgacc gttttcatcc gtggtcattt cttgaacgat
    25381 ttcaccggtt ggactgtctt tgcgaatggc aaacgtatta ccagctaagg aataattgtc
    25441 attccaaaga tcactccctg tttcaactcc gcttttatcc agaatgattt gccctttttc
    25501 ccgtgcgttt tttgaagtca cgtaaatggt ttcgcctgct ttaatggtgg tagtcatggg
    25561 ggtggtatca atcgtataag gcgccggtac tgatttttcg gtaatggtta cttttgtacc
    25621 atgaggaatc tcatccaatg tagcaatgcc ttctttgtca gtagtcacgt cttttgcagg
    25681 taaagttttt ccaaagtcta aatggaaaac cgttcctggt acaacatcac ccgattcttt
    25741 atcgactttt ttgatcttta aagaaccctc cgtttccaca tcaattttga ttgtgtaggt
    25801 attaggttta tcaatcgccc cggccattaa agtctgttgg ccagcctttt tataggcgac
    25861 aggtgttccg gtaccagcag atttcttaag cgtcagcaca ccactttctt ttgaattggc
    25921 atttggggtg attatcaact gattaccatt cacacgataa tcaatgtttg cggcattttc
    25981 cacaacctca tcgaactctg acaaattcag attattcgta tccgtcacag tagtcgattg
    26041 ccctaatact gttttagccg tactattatg gaaggttggt ttcttctgat aatcgtcaat
    26101 ggcttgattt atttttgcct caatggctgc gatatttacg gcactaccat ctgatcgttt
    26161 tattgagtga agggtatacc cattaacttc ttgccagatc atcttctgtg ccacaatatg
    26221 agtatctgca tctgtaccta cttttttcca taaaacggaa actaatttgg ctttatcgga
    26281 catatctggt aacgggtttt tctcatagcc aggagtgaac tcattgtaaa tcgggactcc
    26341 tggttcaata cagaaaatat cttgtttaaa gccgttgtac actgcataat gcggaacagt
    26401 gacttctgac attggacgac catcactaaa agttccttca acagcatatt gatgtgctaa
    26461 atctagttct acggttaccg tttgtggatg ggtagtttca tcagcaaaag taatcgttgg
    26521 agcaattgct ccaacaattg cttgaacagc caaactcaaa atagttaata cacataattt
    26581 ctttttttgt tttttataca tgaaatgttc tcctttcaaa ttaagcacaa aaaaataaga
    26641 tgctcgcttg aacatctttg tttataaagt ctttttaaaa tttttattga ctgaatagaa
    26701 tggatttgag aatgaatgag cctaaaaatt taa
//
LOCUS       DAYYKC010000031        26677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000031 DAYYKC010000000
VERSION     DAYYKC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.031"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHBBW_001274"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHBBW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHBBW_001275"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHBBW_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHBBW_001276"
     CDS             complement(1147..1716)
                     /locus_tag="ACHBBW_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9152971.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACHBBW_001277"
     CDS             complement(1790..3079)
                     /locus_tag="ACHBBW_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152972.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACHBBW_001278"
     CDS             3406..4281
                     /locus_tag="ACHBBW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9152973.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACHBBW_001279"
     CDS             4295..4618
                     /locus_tag="ACHBBW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152974.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACHBBW_001280"
     CDS             4615..5367
                     /locus_tag="ACHBBW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9152975.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACHBBW_001281"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACHBBW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHBBW_001282"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHBBW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9152976.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACHBBW_001283"
     CDS             complement(9831..12017)
                     /locus_tag="ACHBBW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9152977.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHBBW_001284"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHBBW_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9152978.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACHBBW_001285"
     CDS             complement(12239..12679)
                     /locus_tag="ACHBBW_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9152979.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13251)
                     /locus_tag="ACHBBW_001286"
                     /pseudo
     CDS             complement(12753..13251)
                     /locus_tag="ACHBBW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13536..15002)
                     /locus_tag="ACHBBW_001287"
     CDS             complement(13536..15002)
                     /locus_tag="ACHBBW_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9152980.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15313..17394)
                     /locus_tag="ACHBBW_001288"
     CDS             complement(15313..17394)
                     /locus_tag="ACHBBW_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9152981.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17536..17658)
                     /locus_tag="ACHBBW_001289"
     CDS             complement(17536..17658)
                     /locus_tag="ACHBBW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152982.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(17918..18277)
                     /locus_tag="ACHBBW_001290"
     CDS             complement(17918..18277)
                     /locus_tag="ACHBBW_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9152983.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18307..18648)
                     /locus_tag="ACHBBW_001291"
     CDS             complement(18307..18648)
                     /locus_tag="ACHBBW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9152984.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18645..19319)
                     /locus_tag="ACHBBW_001292"
     CDS             complement(18645..19319)
                     /locus_tag="ACHBBW_001292"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9152985.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACHBBW_001293"
     CDS             complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACHBBW_001293"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9152986.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21591..22781)
                     /locus_tag="ACHBBW_001294"
     CDS             complement(21591..22781)
                     /locus_tag="ACHBBW_001294"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9152987.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22802..23329)
                     /locus_tag="ACHBBW_001295"
     CDS             complement(22802..23329)
                     /locus_tag="ACHBBW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9152988.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23376..26165)
                     /locus_tag="ACHBBW_001296"
     CDS             complement(23376..26165)
                     /locus_tag="ACHBBW_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9152989.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26312..26512)
                     /locus_tag="ACHBBW_001297"
     CDS             complement(26312..26512)
                     /locus_tag="ACHBBW_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9152990.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         7474 a         5664 c         4693 g         8846 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattttatgt gtgtattatt ttatttaata cacatcgttt
    15121 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    15181 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15241 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15301 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15361 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15421 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
    15481 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15541 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15601 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15661 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15721 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15781 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15841 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgattgc
    15901 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15961 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16021 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16081 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatatctt
    16141 tggatcgatt tttgtatcga tcgtctggat cgaaaagtcg cctttcgtta atgtgccggt
    16201 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
    16261 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    16321 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16381 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16441 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16501 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16561 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16621 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16681 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16741 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16801 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16861 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16921 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16981 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17041 caataaatac cctatcaagc gaattagttc attaaaatca aataatagca ataaagcgat
    17101 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17161 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17221 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tccgcgtcaa tcgttagttt
    17281 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    17341 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17401 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    17461 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17521 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    17581 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17641 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17701 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17761 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    17821 tcacatggaa gaacttatac aggaaaacgc atagagtcag gtgaaataca tgtatcccac
    17881 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    17941 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18001 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18061 tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt acttctttgc
    18121 caaaaggcgc aaaagaaagg aaggcgatct taaaacattg cataccgatt gggataccta
    18181 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18241 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18301 ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt ccagcatcaa
    18361 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18421 gattagtttt cctgatggtc gctgacccac ccgttcttct gactgttgca cgtccgcttg
    18481 cgcaaaaccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18541 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18601 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    18661 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    18721 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    18781 gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac cagcaatgcg
    18841 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    18901 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    18961 cccgcttttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19021 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19081 aaaatcgata catagtcagg ttgtaaagct aaacaatctt ctcgccagcg tacttgcaga
    19141 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19201 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19261 gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg ttttttcatt
    19321 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19381 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19441 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgct
    19501 ggattcccct tgattccttc attgtttggt ccagagagat cgatcacacc taagcttccg
    19561 tcaatcagac cacttgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19621 cagtaaatgt tcctttacta atatctcttc tagattgctt gggatagatt ggccataact
    19681 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggatcat tgcatgctga
    19741 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19801 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
    19861 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    19921 tttgctttct tctcttatct gctggaaact tcctttatcc cgtatttaaa aaaaaagaac
    19981 tgcgagagtt cgcagttctt ttttttaaat aaacgttgct ctccggccaa ctccttggtc
    20041 acaagtcaca atagtcatca aacatgaaaa aatgtttttt tgaaattact ccttgtgact
    20101 cggagctgaa cggcagacgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    20161 cgatattagc tcaaaaaaca gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    20221 tactcaggtc aaggcactga tttcacaacc tcttatggat aaattcggtt caacaaacgt
    20281 gggaatggaa tagcttcacg cacatggtcg ataccagcaa tccaagtgac tgttcgttca
    20341 aggcctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc aagataccat
    20401 gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa atcatagtct
    20461 gttgcccgtt cgcttccacc aataatttca ccgtaacctt ctggtgcgat catatctgca
    20521 cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt gatggctttt
    20581 ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt ttcatgaggt
    20641 gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag taattcgatc
    20701 gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa gacttctttg
    20761 tctcgtccta gtacatctaa tgcgtattcg caatgatcta aaacattttg tactaagaat
    20821 gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc catttctggt
    20881 tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc acggaaagtc
    20941 ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta taattggcca
    21001 ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc cgtagtgcct
    21061 tctggtgcag aaccagtcaa gatcggcgga tcgattttga caaaatgatg ttgattaaag
    21121 aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg ttgtcttgaa
    21181 gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc ttttggtgtg
    21241 atcggataat cgtggctctc gcttaccact tccacatttg taattccgat ttcataacca
    21301 aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt ttcttggtgt
    21361 agatttttag ctgtttggaa aacttcttcc ggcacttcgc tttttacaac tacaccttgg
    21421 aaaaaggctg ttccgtctct taattgcaaa aatgcgattt ttccgcttga tcgcttattt
    21481 gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc gatgatttga
    21541 atttgttcca cgtatgactc tccttttata ttccttgaat ttcttcagga ttaattttga
    21601 cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt agcgcgatca
    21661 gtggcataac tcatgcggac attttctggt gccccaaagc cttcgccagt cacaagcgct
    21721 acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata tttacacatg
    21781 cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa
    21841 acacccggca gttctgccac aagcggatat aacgtattca ggcgttcttc aaaggcgttc
    21901 cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc atattgactg
    21961 actgctgtcg gattactggt tgactgggaa gcaacagcaa tcattccatt gatgatctca
    22021 gaattcccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt agacacgcca
    22081 ttgatgatga tggtctgatt tttgattgct tctgaaatcg ttgcaattgg agtaaactca
    22141 ttgccattat aaactaaacg gccataaata tcatcagcaa ctatcaaaat atcatttttt
    22201 actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc agtcggattc
    22261 gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc tagctgctca
    22321 acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag tccttctgcc
    22381 agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac ttcatcttgc
    22441 gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc tgttacgact
    22501 gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac gattgcctgt
    22561 cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccatt gcggattgct
    22621 tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa
    22681 ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc tgccaaagtg
    22741 acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat tcctttacct
    22801 tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc aggtaataat
    22861 tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca tcatacatcc
    22921 ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc tgttttgctt
    22981 cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca gatttaggaa
    23041 tgataacggc gatttctttt cctttatcat tcgtacccgt caatgaaaag tacgttttct
    23101 ttcgagtaaa ccagtaaaaa tgatcggctt cctcgagatt tgcatatttc tttgcgattt
    23161 ctactgcttc ttttttcgcc tggaccgccg gacgactgga acgcaagtaa aaaatcgagc
    23221 tgatcacgat gaccgctaat aaaaatacga tcaagccaag caacacgcga ttaatcgttt
    23281 tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt
    23341 atagcagatt ttaatgatga aaaaagacgg cacctttaac ttccttcttc atttttcttt
    23401 aagaattcat ggatatcctg atagatttca tctatagcta gctcttttat cggtactttt
    23461 ttcggtaatg catggatgat tcgtgcacca tattttgcag tgactaatct ccgatccaaa
    23521 ataagtaata cgccacgatc tgtctccgtc cttatcaaac gacctaatgc ttggcgtaac
    23581 ttcaaagctg ctttaggcac ggattcctga taaaaaggat tgatcccctc tgattccaga
    23641 taagcattcc tagctttgac catcggacgt aacgggttgt cgaagggtaa acgtgtgact
    23701 acaacaatct gcaaagcatc tccaggtaaa tctacgcctt cccaaaagct gtcagcacca
    23761 aataagacgc ttttttctga caaaatgaag cgtttcaaaa gtttttcccg actcccgcca
    23821 aatccttgag caagaatttc acgtcctttt tctagcatag acaaatgcat tttctgatat
    23881 accttttgca aaacatcatg agaagtgaat aaaacaagga tcggccgatc ttcttgttca
    23941 atcaatcctg tgagtgctgc ttctaaataa tcgatatatg cttgattgct gacttgatgg
    24001 actgcaggtg cttctttcgg cacatagatt cttgcctgtt tctcataatc ataaggagaa
    24061 gcaatcactt taagaggtgc tgcttcaatg ccaagtcgtt tagagagata ctgtcgatta
    24121 ggtccgatct tcagtgttcc tccaatataa agaatatggt catatcgagg ataccacaca
    24181 gttgacggca acatagctgc ttgaaaatca aggagatgaa aaatcatctg ctgtgctgta
    24241 ttcccgggaa cgaaccaatg gatcaaatga tctcgccagt cttctagcca ttggtccatc
    24301 agcaagtatt gcttgtctaa ttgatcaaat aagctatata aacttgctaa atcgatccgc
    24361 atctggttgg tccaactttc ttggataatc tcaaaagtca agcgcatctg tgattgtagt
    24421 tgttggatct cttgataaaa aagttgcaga tgatccattg ctgttccaga ttccagtggc
    24481 agctgatcca acatttcttt tgtcatcatg acttcttctg gatattgtcc gttaggttgc
    24541 ttgctgctta ctaagttcat taatccctct gccacgcaaa tctgcgcttc gcatagtgca
    24601 gagagttctt ctcgataaat cgacaaccag cgcaaggctt catgttcctt cggcaaataa
    24661 ctttgtatgc ggtcaaataa ctgattcggc tcttccagtt gctgaatttg tttatggaag
    24721 tagttaatac ttagtcgttt gtcatttact ttttcagcaa tttccggcaa atgatgcact
    24781 tcatcaatca acaaataagg actagctgga agcaatggtt cttttcgctg tgtttcttgt
    24841 actaaaaatg catggttgac tatcaaaaaa ttgctttgtt tgattttctt ttgcaagaaa
    24901 cgcacaaagt cttgtttgta aaatgactga ttccttgcta aaaacccagt gcctctgtgc
    24961 gcgacatctg caaagaatgc atggttcaga ttggttaaat gtaattcatc caaatcaccg
    25021 gtcttcgttt gtgttagcca aactaagaca gccatttggt acaaagcata ttgtttttgt
    25081 cccggcggtt ccacaagtgt acctttaaaa cgctgtaaat caatatagtg gcgatagctt
    25141 ttgataatca caggttgtat cggctgatct agcaattgat tcagtaatgg aatgtctttt
    25201 tccatgatct gctgctgcaa aacaagcgaa acggtgctga tgacagctgg tttttcaggt
    25261 gtagccaaat aactcaaagg caacagataa ccaatcgttt tgcccattcc tgtagctgct
    25321 tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagacagc attcatcagc
    25381 ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt
    25441 tttcttggat atgtccgctc gccataatgg acggatgtga atagctccac ttcttttttc
    25501 tgcaaagcta gcccatcgat gatttctaat gagagatcaa ggggctccgg gttttctttc
    25561 atttcttcaa gcacgtggta aatgaaacga ctcgtatcca ttcctgtatg gctacttaat
    25621 cgtgcaatct gctctaatgt gatgatcggc aatttcctca ttcgttcttc gatcagtaaa
    25681 agaagctgac ctgtcacttc ggcatcacta tccgcctgat gaggattctc atgaataaag
    25741 ccaaggcttt ctgacaaatc agctaagcga aaacttggtt ccgtcggcaa gaaaatttgt
    25801 gcaagttcta cggtatcgat gcccggtatc ttcaaactag gaagtccgca tcgcatgagt
    25861 tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat ggtgtctgcc
    25921 aaaagattgt aaatcgtttg ggcgacatct tcaaaataag gcgctttttg gacgcgttga
    25981 ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg atgaatatcg
    26041 atcgaaaaac gtgaagtgat ccgaccagct tcaatcatga cgcagccaaa ttggatgatc
    26101 cgatcttctt ttggattcgt cccagtcgtc tcgatatcaa cgactgcata agttcttggt
    26161 ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata ctacaaagtg
    26221 cagaaaaata ctctcatcgc tttttaagga aaaaaagaca aaaaagccca gacacataaa
    26281 tgtctggact tttattttta atataggtaa attaagcttt agtaacgttt gttgcttgag
    26341 gtccgcgttg accttcttca acgtcgaaag ttactgcttg gccttcttct aaagttttga
    26401 atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt tctgcagtga
    26461 tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc ataataaaaa
    26521 tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat ctggaagatg
    26581 tttatttgaa acaagctacc acagattacc gaacaaaaac tacattctta ctatatcata
    26641 ccgtttacaa aattgccaga gtttttcctt atttttt
//
LOCUS       DAYYKC010000032        26615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000032 DAYYKC010000000
VERSION     DAYYKC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.032"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHBBW_001298"
     CDS             <1..1152
                     /locus_tag="ACHBBW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM9152991.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHBBW_001299"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHBBW_001299"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9152992.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHBBW_001300"
     CDS             2728..3003
                     /locus_tag="ACHBBW_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9152993.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHBBW_001301"
     CDS             3009..3776
                     /locus_tag="ACHBBW_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9152994.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHBBW_001302"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHBBW_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9152995.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHBBW_001303"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHBBW_001303"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9152996.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHBBW_001304"
     CDS             7920..8222
                     /locus_tag="ACHBBW_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9152997.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHBBW_001305"
     CDS             8225..9388
                     /locus_tag="ACHBBW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9152998.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHBBW_001306"
     CDS             9414..12842
                     /locus_tag="ACHBBW_001306"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9152999.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHBBW_001307"
     CDS             12863..13987
                     /locus_tag="ACHBBW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9153000.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHBBW_001308"
     CDS             13968..14414
                     /locus_tag="ACHBBW_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9153001.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHBBW_001309"
     CDS             14398..14736
                     /locus_tag="ACHBBW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9153002.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHBBW_001310"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHBBW_001310"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9153003.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHBBW_001311"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHBBW_001311"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9153004.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHBBW_001312"
     CDS             15893..16936
                     /locus_tag="ACHBBW_001312"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9153005.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACHBBW_001313"
     CDS             17095..17823
                     /locus_tag="ACHBBW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9153006.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACHBBW_001314"
     CDS             17939..18184
                     /locus_tag="ACHBBW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9153007.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACHBBW_001315"
     CDS             18181..18399
                     /locus_tag="ACHBBW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9153008.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACHBBW_001316"
     CDS             18535..19074
                     /locus_tag="ACHBBW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9153009.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACHBBW_001317"
     CDS             19067..21340
                     /locus_tag="ACHBBW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9153010.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACHBBW_001318"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACHBBW_001318"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9153011.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHBBW_001319"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHBBW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9153012.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACHBBW_001320"
     CDS             complement(23167..23436)
                     /locus_tag="ACHBBW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153013.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACHBBW_001321"
     CDS             complement(23698..23826)
                     /locus_tag="ACHBBW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153014.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACHBBW_001322"
     CDS             complement(23906..24361)
                     /locus_tag="ACHBBW_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9153015.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACHBBW_001323"
     CDS             complement(24499..26427)
                     /locus_tag="ACHBBW_001323"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9153016.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4612 c         5619 g         7523 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaggt gttct
//
LOCUS       DAYYKC010000033        25940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000033 DAYYKC010000000
VERSION     DAYYKC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.033"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..2421
                     /locus_tag="ACHBBW_001324"
     CDS             361..2421
                     /locus_tag="ACHBBW_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9153017.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2504..3073
                     /locus_tag="ACHBBW_001325"
     CDS             2504..3073
                     /locus_tag="ACHBBW_001325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153018.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3248..4054)
                     /locus_tag="ACHBBW_001326"
     CDS             complement(3248..4054)
                     /locus_tag="ACHBBW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153019.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4192..5706
                     /locus_tag="ACHBBW_001327"
     CDS             4192..5706
                     /locus_tag="ACHBBW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9153020.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(6109..7092)
                     /locus_tag="ACHBBW_001328"
     CDS             complement(6109..7092)
                     /locus_tag="ACHBBW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9153021.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(7097..7744)
                     /locus_tag="ACHBBW_001329"
     CDS             complement(7097..7744)
                     /locus_tag="ACHBBW_001329"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9153022.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7829..8461)
                     /locus_tag="ACHBBW_001330"
     CDS             complement(7829..8461)
                     /locus_tag="ACHBBW_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9153023.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8477..9028)
                     /gene="coaC"
                     /locus_tag="ACHBBW_001331"
     CDS             complement(8477..9028)
                     /gene="coaC"
                     /locus_tag="ACHBBW_001331"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9153024.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9025..9762)
                     /locus_tag="ACHBBW_001332"
     CDS             complement(9025..9762)
                     /locus_tag="ACHBBW_001332"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9153025.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9759..10748)
                     /locus_tag="ACHBBW_001333"
     CDS             complement(9759..10748)
                     /locus_tag="ACHBBW_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9153026.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            11006..11548
                     /locus_tag="ACHBBW_001334"
     CDS             11006..11548
                     /locus_tag="ACHBBW_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9153027.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11565..12650
                     /locus_tag="ACHBBW_001335"
     CDS             11565..12650
                     /locus_tag="ACHBBW_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153028.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12662..13468
                     /locus_tag="ACHBBW_001336"
     CDS             12662..13468
                     /locus_tag="ACHBBW_001336"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153029.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13469..14296
                     /locus_tag="ACHBBW_001337"
     CDS             13469..14296
                     /locus_tag="ACHBBW_001337"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153030.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14293..15366
                     /locus_tag="ACHBBW_001338"
     CDS             14293..15366
                     /locus_tag="ACHBBW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153031.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15794..18490
                     /locus_tag="ACHBBW_001339"
                     /pseudo
     CDS             15794..18490
                     /locus_tag="ACHBBW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            18555..19055
                     /locus_tag="ACHBBW_001340"
     CDS             18555..19055
                     /locus_tag="ACHBBW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9153032.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            19067..19561
                     /locus_tag="ACHBBW_001341"
     CDS             19067..19561
                     /locus_tag="ACHBBW_001341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9153033.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19582..20391
                     /locus_tag="ACHBBW_001342"
     CDS             19582..20391
                     /locus_tag="ACHBBW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9153034.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20388..21209
                     /locus_tag="ACHBBW_001343"
     CDS             20388..21209
                     /locus_tag="ACHBBW_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9153035.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21260..23359
                     /locus_tag="ACHBBW_001344"
     CDS             21260..23359
                     /locus_tag="ACHBBW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9153036.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23356..24474
                     /locus_tag="ACHBBW_001345"
     CDS             23356..24474
                     /locus_tag="ACHBBW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9153037.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24513..25391
                     /locus_tag="ACHBBW_001346"
     CDS             24513..25391
                     /locus_tag="ACHBBW_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9153038.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25537..25734
                     /locus_tag="ACHBBW_001347"
     CDS             25537..25734
                     /locus_tag="ACHBBW_001347"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9153039.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25718..>25940
                     /locus_tag="ACHBBW_001348"
     CDS             25718..>25940
                     /locus_tag="ACHBBW_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153040.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNK"
BASE COUNT         8225 a         4501 c         5460 g         7754 t
ORIGIN      
        1 ccaccgactc cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga
       61 catttaactg aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt
      121 agctgattta tgtttttttc aaagaaaagg taactagata taactagata taactagata
      181 tattacttaa caaaattaaa aagtacataa gttattattc cgattttctt gaaacaataa
      241 aaaatcaaaa aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga
      301 aatcatctta tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa
      361 atgtgttcaa aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa
      421 cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta
      481 tctgtaggtg tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta
      541 cgtacctctg tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat
      601 gcaaatggaa aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca
      661 acgataaacc actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc
      721 agtgtgccgc tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa
      781 cagatcactg atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa
      841 gtttcttatg tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg
      901 gcagggacaa aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca
      961 caaggagata tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca
     1021 ggaacatcta ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca
     1081 tctgctgtga tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta
     1141 agaacaggag agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga
     1201 acttcaggaa ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga
     1261 gaatatcccg ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca
     1321 gctgtagttt atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat
     1381 agtcaaacaa ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct
     1441 gatttgcgcc tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat
     1501 gaacaaaatg ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga
     1561 aaaagtcact ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag
     1621 caatcaggag gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa
     1681 ggtcatgatc ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca
     1741 gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa
     1801 gtaccattgg aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt
     1861 tatcatgacc cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca
     1921 ttaaacaaac agatagatca aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa
     1981 aaacaacaaa tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta
     2041 gagttagcaa gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct
     2101 catttggccc cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa
     2161 caatcagaaa aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag
     2221 aaggaaacga agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg
     2281 caagtaccac ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct
     2341 ttaggagatt tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt
     2401 gacgcagatg actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag
     2461 tcatatgtta tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt
     2521 accttacgca gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt
     2581 agcggaaggt tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat
     2641 tgagacttcc gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa
     2701 ctttgctcct tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg
     2761 agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt
     2821 ttttcattgg ttagcagtga aatcagatta ccaaggaaaa gggctcgcaa aagcattgac
     2881 tgccagagtc ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac
     2941 acagacatgg agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc
     3001 tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa
     3061 attagataaa taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag
     3121 tcattcctca tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata
     3181 cattcaaaaa agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta
     3241 gcatgtttta tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt
     3301 aatcgtttcg actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa
     3361 caaataaatc cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg
     3421 ttttatctgt tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa
     3481 catgatagac agtgtttccg tctttactta actccacact attgatttgc tggatgatcg
     3541 tatctttttg catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca
     3601 taacaccaac tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt
     3661 tcatggtttt tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta
     3721 aaaatccgct gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac
     3781 ttaactctcc taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat
     3841 ttggaatctg tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg
     3901 tcagtaataa aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa
     3961 taacgacgat gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg
     4021 tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt
     4081 aattatacta agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga
     4141 tattgtgaaa aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa
     4201 ttagttgctg gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat
     4261 cagaaaaata cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc
     4321 gcttctgaac aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt
     4381 attggagctg gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat
     4441 ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt
     4501 atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc
     4561 ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac
     4621 tttgtcgatc actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac
     4681 aacttaacga tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg
     4741 gctattggtg gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct
     4801 atttttgtca acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg
     4861 actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc
     4921 acaggcggtt ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat
     4981 tatattacga caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta
     5041 ggtggacaaa ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag
     5101 ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg
     5161 aaaagatttg tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg
     5221 cctgaaaaat cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt
     5281 gaattttatg accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa
     5341 aaaatcgatt taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt
     5401 gcggagaaaa aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct
     5461 ccgaattatt atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa
     5521 atcaatacga acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct
     5581 gctggtgaag tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg
     5641 gagatcatta tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat
     5701 gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga
     5761 gggtcagctg attgaacact gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag
     5821 taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggttg gacttattac
     5881 cgcagatgca agcttttgaa caccgtttat tcggaaaaag agcgtgggac aaaagtaaag
     5941 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaaga aattccttcg
     6001 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     6061 ttggagttaa acgcttctgt cccagcctct tttttgatca gcatgatttt attcagttat
     6121 cttcaaaatg atttttccga tgtttttgtt tgcttccata tattcatgcg cttgtttgac
     6181 tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg
     6241 cccagctttt tgcataaaag tttgtgcgag ctctgcttta tactgatcac ttctaggtgt
     6301 caataatgta ccaagcagtt ggacacgttt cccaatcaat acaccaaggt ctatctcagg
     6361 cacgatggtc ccacctagca taccaatcaa gacccaacgt ccatcaacgg ctatagaagc
     6421 taaattcttt tcccagtaag aagcgccgat gaaatccaaa ataacatcga ctccccgacc
     6481 ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt ttatagttga tcaattcatc
     6541 tgcaccaagt tctttacaaa aggcgagttt ttcttctgat cctgcagtaa cgatgatttt
     6601 cgcgtgagaa agctgtctag ctaattggat cgctgctgtt cccacaccac ttgctcctgc
     6661 atggatcaag acagtttcgc cttcttgtaa ttttccaagc cagtaaagtg tttgataagc
     6721 agtcaaaaaa acttctgaga ttccagcagc agaaacatag tctagcgtat cagggagaag
     6781 gattgcacga tcggcaggca tcacagcata ttcggcataa ccacctagat tgactaatcc
     6841 gtatactttt gtcccaggag taaaagtgga gatatcactt ttattctcaa ctacctctcc
     6901 agctacttcg actcccaaaa tagggtaggg aggtttcaaa gagactgatt ctcttttcat
     6961 aatatctgtt cgattgacag ctgctgtatg gactttgatc agtaattcgc cttgttttgg
     7021 tttaggaaca ggcacttcaa tttgttctaa tacatctaca gaaccaggat gcttgattga
     7081 aatggcacgc atggatctat tcctcctttg cccattcttg gaatgctgta ttcaactctt
     7141 tgctaccttc agaaagtact agagtcgtac gagaaatatc cgtttcttcc actagagcgg
     7201 caagtacagc agcaactgta gagcgagtga tcgtttttga tgccttctca ttaggcttag
     7261 ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt gactggttga agaatgacaa
     7321 aatctaagtt agttcgtttc agccattcat ctgcataatg tttagtaata taataatctg
     7381 tcatggactc aggccatttt gctggttcat ctgcataaac agcactgacc atcagatagc
     7441 gagagacatg cgcattttca gctgcgatca ctgttttgat tgcgccatcc aagtctactt
     7501 gcagaagatt ctttccttga gagccggcag caaaaatcaa tacatcgatg cctttgaatg
     7561 cttcggtcat tttttccacg gaccaagtta aatcaaaaga gacatacgaa acattctctt
     7621 tttttactaa tgtttgggaa gcaacatctc tcacccctgc aaaaatttca tgggaacttg
     7681 gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt
     7741 tcatttggat catcctttcg acgtcaaatc tttccattca atagtaacac caaaagagaa
     7801 aaaaatcaga gatcggctac gatgactatc agttagtaag agaagggtgc ttgaagtatc
     7861 ttaacaagac attgcatact gctgcagtcg caagacctgc gacgattcct tcaggtatac
     7921 cacttgtaaa aataaccgct agaatagctg aataaacagc atttaaattt gttccgatga
     7981 ctttggcata atcttgctgg aataaaaagt agatcatatt catcactaaa atcgtattgg
     8041 tcaatgaacc ggaaagtcca gctaagaata aagaaacgga atctgctttt tgtttcatta
     8101 gtttgcgcag acctttataa acgaaataag gaaccacccc aatcaagatg cgaggaataa
     8161 acgcgatcag caaggctttc cagcttccat gttctgttcc gatgactggg ataaacggag
     8221 aaaagacaaa agacaaaggt gtttggataa ctgtgctacg gatcatgctg atcagtccaa
     8281 atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac gatcgaagca atgattacag
     8341 gaatatgcat ggttgttgca ttgattggac cgatagggat aaaacctaaa ggcgtaacag
     8401 ctaataaaat caaaatagct aaaaacatcg cagttaatgt aaagtttttt gtatttttca
     8461 taagtactcc ttttttctaa gcatttcttt cattaagagt cagcatgacc tgttcgatga
     8521 tcgtctgaat atcagctaag gcaccttttc caaaatctcc acaagctaaa agagattctc
     8581 ttggttcaat ttcctgatag ccgacagatc tcaatgtttc cagattacgc tgagtgatcg
     8641 ggttttgata catattcgtg ttcatggcag gggcaatcaa ttttggtgtt cccactggaa
     8701 gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa
     8761 tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg ttttgctagt tcgatatgat
     8821 tgattacctg cggatttgat tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag
     8881 attgcaaggt taatggcgtg ataaaatgtg tgctgctttt agacataatc acatctacct
     8941 gggcacctaa tttgacgaat tgacttgtaa gatcagcgct tttgtaagca gaaatacttc
     9001 cagtaacgcc tagtaagatc tttttcattt tgattcctcc agtctaattt tctcagtaat
     9061 caatgcagca atctcagatt tactttgagc ttcttccact gttccatctt tagataagag
     9121 atagccgtgg tgtgtctcgc catggatttc cgtcaaatca tttgcgaaaa taaaatcagt
     9181 tttgttattt atcagactat tttttgcgac tgccagaaga gattcctttg gtacatctac
     9241 taatagtttg aaaccaatga tttttgcttt tggctgccat tcacgtagat aagaaatgat
     9301 ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg ttagaagaga tctttgaaac
     9361 agtggtatct gtctgttcat tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga
     9421 agtgggtagg ttttttgcca gctgttcttc tgaaaaacta aaagcaggag taaaatcact
     9481 gactgccata ctgtggataa tcgcatcgta gtgttctttt ttcgttagtt cttctaattg
     9541 taaaaataag tccagtgcag attcgatcgt ataaatcgtt atattggagt gtcttttcgg
     9601 tttcagtgct tgtttcgtag tcacgtaatc gattgtggta tccggattgg cagctaaata
     9661 atcagctaat gcttgcccta atcgtcctgt cgaatgattt gtaatagcac gtacttgatc
     9721 gattttttca gtcgttcctc cagctgtgat tagaatcttc atttatggtc acctacattt
     9781 actagtgttc ttccttggtg tgttccagca agtagtttag atgctgtttc aggcaattga
     9841 tccagagtaa tctcttggac gaccagttca tctgcaatct gcaaatccgc catctgttgc
     9901 cataaagaaa gacgttgatc tattggtaca ttgacagaat caattccaat caactggatg
     9961 tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga ttcccccagc attgccacat
    10021 aaagctactg caccgttata agagatcaat ggaagaagac tgcttagctg ctcgccacct
    10081 actgtgtcga tgacatagtc gatttgctgt tttcctaaag cttttgttgt ctctggcaga
    10141 aattcatcag gtcgtacgac ttgcaaagca cctagacttt ttagccatgt gacagactct
    10201 tcttttctgg aaaaggcaat gattgactgg aatcctagtt tatgtaacag ggctatcgct
    10261 gtactgccta caccgccaga agcaccagta acgacaactt ttttatcgga ggtcatacct
    10321 tgacggatta aagcgttcac acacaacata gcagtaaacc ctgctgtacc aaggatcatc
    10381 gcttgacgca gcgtcatatt ttttggtaga ggaactaacc agtctcctgg tactttttga
    10441 tattggctat aaccgcctgg atgggtgacg ccaagcccaa aaccagtagc aagtacaaga
    10501 tcttcttttg aaaaacgtgg attgttactt tggacgacag ttccggataa atcaatccct
    10561 ggagtcatcg gatatgttcg tatcacctga ccagactcgc tcattgccaa tgcatcttta
    10621 taattgacat cagaataggc aatttttacc aagacttcat tttcagccaa ctcagtaaat
    10681 ggcagatcac tgattgctgt atggaaaaca ggttccttta ttacctgaaa agatcgaaaa
    10741 gcgttcattc gtgagacact ccttttaaaa ttttctttta tcataccata aagcctactc
    10801 ttttattctt gttcttccgt aaatttagac aaaaattagg tgaaaaataa agaatcaaac
    10861 aactagaatt tattccaaaa gataaagaaa tattcagcaa aaaaaatttt acaaagggtt
    10921 gcctttttca ataaatttcg tatactaata cgagtggtag taaacaggaa gtttactatt
    10981 agaaaacttc gattcggagg aaattatgga aattggtgag aagttaagaa atttgcgcat
    11041 ccaaaagaat ttgacacaag aagaactagg cgaacgcaca gatttgtcaa aagggtatat
    11101 ttcccagttg gaacgagatt taagttcacc atcaatggaa acattctttt ctattcttga
    11161 agtcttaggt gtgactccag aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt
    11221 ttattcaaaa gaagatcata cggtttattt cgatgaagaa aacgggtatg aattggaatg
    11281 gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc
    11341 aggagaatat aagacgtttg aaccttcatt atctgaaacg tttatttttg tattagaagg
    11401 ggaattagag ttaagcttag gagagacggt ctatgtggca aaagcaggac agtcgatcta
    11461 ttatcaagcg acaaaacaac atcaattaag gaatcatctc aaaaaacgaa caaaagtatt
    11521 gattgttgca acgcaatcat atttgtaata tagggggtaa aaacgtgggg aaaccaatca
    11581 tttcatttga tcaagtagtg aaacgctatg atgatgaaac agtattaaaa aaagtaagtt
    11641 ttgagatcga acaaggaaaa ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa
    11701 cgatcctacg tattatagct ggattcaccg aagctacaga aggggacatc tattttgaag
    11761 ggaaacggtt gaatgatttg ccagctaata agcgacaagt aaatacagtc ttccaagatt
    11821 atgcactttt tcctcatatg aatgtttttg ataacgttgc ttttggattg aagatcaaga
    11881 aaatgtccaa atcagatatc gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc
    11941 ctggatacga aaaacgggag attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga
    12001 ttgcacgagc aattgtaaat gaaccgaaag tattgctatt agatgaaccg ttatcggcgc
    12061 ttgatttgaa actacgtaca gatatgcagt atgaactgcg ggatctgcaa caacgattag
    12121 gaatcacgtt tatttttgtt actcatgacc aagaagaagc gttggcaatg agtgatgaga
    12181 tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac acccgtagat atttatgatg
    12241 agccaatcaa tcatttcgtt gctgattttg ttggggagag caatatcgtg gacggtgtaa
    12301 tgatcgaaga taatctagtt gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga
    12361 tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga ggatttgacg atcacgacac
    12421 cagataaagg aaaactagtc gtaacggtcg atactcagct gtttcgtggt gtccattatg
    12481 aaatcatctg ttatgatgaa cagcaaaatg aatggatggt ccattcaacg aaaaaagcta
    12541 aagaaggaag caaagtagga ttggcctttg agccagaaga tatccatgtc atgcgtttca
    12601 acgagtctga agaagaattc gatgcacgtc ttgacagcta cgaagaatag gggggacaag
    12661 aatgaaaaca atgcgcagga tctattccgt cccttatatt ttgtggctcg gtttattcgt
    12721 gatcgcgcct gtattaatga tcatttatca atcttttttt gatatgaatg ggcaattcac
    12781 gctgaataat tatctatctt attttacgtc ggggacatcg ctttctatga cgatcaattc
    12841 tgtatggtat gcatttttga tcacgttttt cacgctgatc atcagttacc cgactgctta
    12901 ttttttaaca aaactgaagc acaagcaatt atggctgatg ctgattattt tgcctacatg
    12961 ggtgaatctg ctgcttaaag cttacgcatt tattgggatt ttcagtgtgc acggcagcgt
    13021 gaatcatttc tttgatttta tcgggatcgg gccgaaacag atattattca cagacttcag
    13081 ttttctgttc gtggcaacgt atatcgagat tccatttatg attatgccaa ttttcaatgc
    13141 attggaagag atgaatcctt ctttgatcag tgctagtaga gatttaggcg cgaatagctt
    13201 tgagaccttc cgacgagtga tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca
    13261 agcagtattt attccttctc tttctttatt catgctgact cgtctgattg gcggaaatcg
    13321 ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg gttacacaaa actgggggat
    13381 gggttctaca atcggtgtga ttttaatcat tgctatgttc atcgtgatgc tcctgacagg
    13441 cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt aaatggtcgt atctttattt
    13501 agggatcgtc tttttattcc tatatgcacc gatcttctat ctgatttttt attccttcaa
    13561 tgaaggagga acgatgaaca gctttacagg atttacttgg gaaaattatg aagctgtttt
    13621 tgaagataca cgtttgatca ttatcgtatt gaatacgttc atgctggctt tcttatctgc
    13681 attgatcgcc acgatcatcg ggacatttgg ggctatggga atctattaca cgaaaaaacg
    13741 acgtgcacgg acagcgttgt tgagcatgaa taacatctta ttagtctctc ctgatgttat
    13801 tatcggtgct agtttcttga ttttctttac actactagga ttcggattag gttttcagag
    13861 tgtattgctt tcccatattg ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa
    13921 attgcaggaa atgaacgatt caatggtcga tgcggctaga gaccttggtg ctaataattt
    13981 ccaagtagtc aaaaatatca ttttgcctta tctttctcca ggaatcatag ctggatattt
    14041 tatggcattt acctattctc ttgatgattt tgcggttaca tttttcgtca caggaaatgg
    14101 ctttacgacg ctctcagtag agatctattc tcgtgcaaga caaggaatca gtttagaaat
    14161 caatgcgcta agtgcgttag ttttcttgtt ctccatgata ttggtcattg gatactactt
    14221 tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac agaagagtac gcgagggggt
    14281 ggcacggctg cgatgaaaaa attacaatct ctgtttttag gtattctcat cattattgcg
    14341 gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta
    14401 cttaccatct ataactgggg agattatatc gatcctagtt tgatcacgaa atttgaaaaa
    14461 gaatatggat acaaagtgaa ttatgaaaca tttgattcta atgaagcgat gttcacaaaa
    14521 atccagcaag gtgggacaaa ctacgacatc gctataccaa gtgaatatat gatccaaaaa
    14581 atgatcaaag aaaaactagt acttccatta gatcattcga agatcaaagg attaaataat
    14641 attgatccac gatttttaga cttggatttt gatccagata atcagtattc gattccatat
    14701 ttttggggaa ctttagggat cgtatataac gataaattct ttgatgcaaa tcaaattaag
    14761 cattgggatg atttgtggaa gccggaatta aaagacagtt tgatgctgat tgacggtgca
    14821 cgagaagtta tggggctttc tttgaatagt ttaggctatt ctttgaatag taagaatatg
    14881 gcacagctga cagaagcagc aaataaattg aatcatttaa caccaaatgt caaagcaatc
    14941 gtggcagatg agatcaaaat gtatatgatc aatgaagaag cctccgtggc agtgacattt
    15001 tctggcgaag ctgccgatat gatgtgggaa aatgaacatt tacattatgt gatcccacca
    15061 gaaggatcca atctttggtt tgataatatc gttatgccaa aaaccgtgaa aaataaagaa
    15121 ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa atgcggcaca aaatgcagag
    15181 tatattggtt attctacacc aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg
    15241 tcggacgaac aattctatcc atctgatgac acgatctccc atcttgaagt ctacaaagat
    15301 ctaggatcga aatacctagg catctataat gacctattct tagaattcaa gatgtatagg
    15361 agataatggc aaaactcagt aagaatgggg acttatcaac gttctaaaga gctaaaaatc
    15421 gtcctttttc tatctgagtt ttccaatctt ctaccaaaag cggatgaaac gccgcttttt
    15481 ttgtgttatt ttattttttc taccacgttt ttctaccaga gaaaagtgga caaaaccatt
    15541 gtaaaatagg gcccgagacg ggctagtaaa tctaagattg aaaaagttac agaaggataa
    15601 agtaattgga aactatgcaa atatacttaa cccttctgta atctattaat aaaactaata
    15661 tatgaatcca gttcaatatt tgtagctatg aaaaaacata aaaatcatat tagataaaaa
    15721 ctaaattggt agataaatta ttttgtcaga tatctgaggc aaatattcga ctatttgtgt
    15781 ttcaaagtaa aaattggaat acaaatatag aatagacata ttgcagccga tagtttatta
    15841 tgcttttaga cacattaaac tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt
    15901 tgatgagctg atacgactaa cagggaatat gtcttctgtc actgccgaga aaatttcgaa
    15961 gaatctaggt atctctcgtc agaatgccag ccattatttg acgagattag tggaagataa
    16021 aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag cctttagatg aatatgcggt
    16081 tgtagataat acggagcaaa taaacgaagc ctttcattct gtagttggtc atgacggaag
    16141 tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa tatccgccta acggactcag
    16201 tgtattaata aatggagcca cgggtgttgg aaaaagtttt ttagccacaa aaatttttga
    16261 atacgctatc catgagcaaa ttattgaaaa agatgcacca tttgctatct taaactgtgc
    16321 cgattatgcg gacaatcccg aactgctttc ggcaacactg tttggataca agaagggggc
    16381 ctttacgggg gctgaaaagg ataccgatgg tttattggca acggccaaca acggctatct
    16441 ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa gaaaagctct ttctatttat
    16501 tgatactgga aactatcgtc cagtcggtga aaatgtaaat tggcattctg ctaaggttcg
    16561 ttttattttc gccacgacgg aaaaaggtga aaattattta ttagacacct ttgatcggcg
    16621 tattcaaatt agtgttatgt tgccaacttt tgaagatcgc cccattcgag aacgattaga
    16681 gctgattcaa ttattttttc aaaatgaagc ggatgtttta caaaaagata ttattgtttc
    16741 aagagaagcc ttgtctttaa tgttatctca tccctttagt gggaatattg gtaagctgaa
    16801 gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga acacaagaag aaccattaac
    16861 gattgggaaa aatgagatct tgattcagtt aaatatgctg gaatccgaag tagaaagctt
    16921 gccgattgct agtttattga taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc
    16981 gtttgattat gattcgattt tggaaaaaat tactcagctg ataaatgaag gtaatttcaa
    17041 tgaaaatttt tcttcgataa agaatgaagt acagcatttg gtgcatcaca ttgataagga
    17101 ttctttttta tcaatgagta gaaaattgat gattcagatg tttagaaagg tctgggtatc
    17161 cgttttagga aaaacgtacg gattgacgac gagtggacct gttgcagaaa ttattagcag
    17221 atcctatgtc atttccccaa gctataagac agacttaacc gggataaatc gtacattagc
    17281 tgcaaatcta cctcggacga cttacttatg taatcagttc ttggagcatt taccaccgct
    17341 ctcagaaaag gacgagaact ttctaagact gcttattgtc gtgactttat ctgattatgt
    17401 agatgaatcc atcgagctga agggcctgtt gcttgctcac ggagatagta cggctagcag
    17461 cattcaagca gtagtgaatc aattatgcgg caattatgtt tttgaagcgt tggatatgcc
    17521 agtagagagt aatgtgtcag agattgtcga atcgacaagg aatttcgtga aacggcaagt
    17581 gaatacagat agtctagtgc tgatcgtaga tatgggctct ttaaatcaaa tttacacaca
    17641 gatcaagaac gatttaaaag gggagttact tcttctgaat aatttgacga cttccattgc
    17701 acttgatatt ggcttgaaga tgacgatgaa tgcgccattt aaatagattg cggaggatgc
    17761 agcagacaag tattcaatca atgttcaata ctttgaaggc ttttctcaaa gtagcaacat
    17821 tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa ttgaaagatg tttttcgtca
    17881 tcatttatcg gatgaatcca tcgaagtttt cacgaaggac tacagagaac tacgtgaatt
    17941 aatcgaccaa aatgatgaaa cttattttga caagacaaaa ttggtcatca cgacttcgga
    18001 tcttccacgt tccttttcaa ttcctaatat taatgtttat gacatcttag acggtgatgg
    18061 agcagccaat ttggagcata ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt
    18121 gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct
    18181 gaacccaacg atcgtgatga gtgaagtgga aacagtcatt tctaaatatg aaaattacta
    18241 tcatttcact ttaaatggcc gtgtaaaact aaatctctat atgcatacgg cattgatgat
    18301 ggaacgttta ttcttggctc ggacagaaaa atctgaagaa ccgaattatg atttgaaacc
    18361 agatgaagat gaattttatt atgtttctag aagtattttt caatcaatgg agatgaaata
    18421 taattttaaa gtcaatgctt atgagctttc gctgctctat gaattattag gaccgtatat
    18481 tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt tttaggttag acactttttt
    18541 taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt tggcacagga attgcttata
    18601 taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa aattctaata gcgactcacg
    18661 gtcacttagc ttcaggcttt cttagcagta ttcaattact gacggggaag gttcaagaga
    18721 ttacggtgat taatgcttat gtggatgagt gtgattttga acaggagttg aatgtatttg
    18781 taaaccaggt gaacgagcag gatcagattt ttgtttttac cgatttattt ggcggaagtg
    18841 tcaatcagaa gattaccaaa acctttttgg agaaagagat tgctgtaact attttgtctg
    18901 gctttaatct gccaattctg ctcgaaatag tattggcaac caaggaatta acaaaggagg
    18961 atattcagga actggtaaat aaatgccaac aggaattaaa gatgaccatt cttgatgagg
    19021 tacatgtagt aaatgaagag gaagattttt tttaggagga agaaaaatga ttaaagcagt
    19081 gcgaattgat gaacgattaa ttcatggaca agtagcgatg acttggacaa agagtctaaa
    19141 tttaaccggg ttagtagtag caagcgatga ggcggctacc aacgatattc aaaagatgac
    19201 attgaagatg gctgcaccat cgaatgtcaa agtcattatt aaaacagttg atgacgcgat
    19261 tcatttgtta aaagatccgc gggcgaagga catgcgcttg atggttttag tccctaatgt
    19321 caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg
    19381 gaatgctggg aaaatgacac cgccatcgga agataagaaa accttatcaa aggaagtgat
    19441 gttaacgacg tcagaaattc aagacttaaa agaattagtt gaactttatc cagataccgt
    19501 ttttcagcct acaccggcga tggaaaaacg ttcagctatc tctattttga gtgattttta
    19561 gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag tcgctttggc cgtttttatt
    19621 ggagttgctg gacatgaaat gttcgggatg tccatgctga gtcgtccgat tgttgttgct
    19681 ccgttagcag gagcgttgat gggggatttg cagacggggt tgattgtcgg ggcgtcacta
    19741 gaagccatct ttatgggtgt cgtcaatatc ggaatctcta gtacggcgga gccttctttg
    19801 gctgctgggc tggctacagc gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt
    19861 cccattgtct ttccactagc agtattagga ttacagttaa tgaatatgat tttttctttt
    19921 gtatgtggac cgatggctgc taaatttttc aaatttgcaa aatctggtga tcaaaaagga
    19981 atcatcaatc tgcacttctt tatgtggttt gtccactatg cgctttatgc tcttattcca
    20041 ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg tcttggataa tattccagag
    20101 gtcatcatga acggattaac agtcgctgga aatctgctgc cggcagttgg gatggcgatg
    20161 ctgttacgga tgctttggag taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt
    20221 atggcgtatc tcgagttacc attgattgca attgccgcag ttggaacgat tattgcaatt
    20281 gttatagccc aaagagatct acaactaaaa aatatatctg ttcatgcagt tcaaagcgat
    20341 gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt tctttcaatg aaattagctg
    20401 atggaatttc taaagatgag aaaaaattgg ttcggaagat gttttggcgc tcgatgacta
    20461 tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc
    20521 ccttcattaa tcatttctac aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata
    20581 cacagtattt ttcgacgacc attccttgcg cgtcctttgt catggggatt gcggcatcga
    20641 tggagaagga aaattctgaa aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa
    20701 cgagtttgat gggaccattg gctggaattg gcgattcatt gttctgggga gtttggcgtg
    20761 tcgttaccgc gggactagcg atcaacttag ccaatgccgg aaatattttg gcaccaatta
    20821 tctttttagt tatgttcaat atacctaatt acttgatgcg atactatggc ggcgttttag
    20881 gctattcatt aggctctaaa tatattgaaa agctgtatgc gaacgggtta attgaaattc
    20941 tgacaaaagc tgctagtatc ttgggtctga tcatggtagg tgcaatgacg atcagtatgg
    21001 tcaaattcaa taccgttttg agcttttcaa tgaagggcga agaactaatg aatgtccaag
    21061 aaatcctcga tagtattttt aaaggaattg taccgttatt ggtaacattg agctgtttca
    21121 aattactaga taaaaaagtc ggcgtcatca aaattatttt tggaatggtc gtcttgggaa
    21181 ttttattgtc attacttgga atcgcatagt aggaagctgc agctactagg ctgcagtttt
    21241 tttagcaagg agggaaaaga tgactattaa tgaagtaatt tattcaccca aacacccatt
    21301 ttttaatgct gagttaaacg acggaaatgt gactacttca aacgaataca gctactatcg
    21361 gaatattcct gatgtaaagg gattgacctt cgatgtaagt aatcaaaaga cattagcaaa
    21421 ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat cgagaaaatt tactaactga
    21481 ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa accaattcgt tatccttaaa
    21541 ggttgaagta aacgggattg tctaccattt gtcagaatcg gatcatcgta ttgaagtcga
    21601 tgtgattcaa gactgtttgc ctagatatat tcatcattat tcgacgttta aggtcattgt
    21661 agtatccttt gctcccatac tggctaataa gcgtttaagt atgcttgttc aacaggttta
    21721 cattgtgaat gaaacacacg aaagccttca agtttcagtg aaagatgtgc cttcgtatca
    21781 agagaagtat tcggatcagc aaaatgtgtt gatttctcaa aaaggaaata aggacacgat
    21841 cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg gacccaaatg cttttgaaga
    21901 agtaatgact tttcaggaat ctgatatcga aaaatggctg aaggaaacat tgaactattt
    21961 tgctcagcta tttgggcagc tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg
    22021 tgcactttat caaagctttt ctagtttcgg tatgaatcga cagggacagg tcgtcggcag
    22081 caattggggc agctatcctg ctactaatcg aatttggaat aaggatatgt attacgcatc
    22141 attgccattc accatgtttg aaccggagtt atgtcaaaaa acggttctat ggtttgatca
    22201 atatggcgtt aagtttcctg gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa
    22261 ttcgttatcc agtgcgttac tggctagtct ttattttgaa catactgggg atacaaaatt
    22321 ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga attattgagg acatattagc
    22381 tcaacgtcgg tcaggggaac caatgctttt tccatctgtt tggttatcag acgcctttgc
    22441 acttggaaag tatcataccg gcagcaatct ttgtctttgg aaggcctgta ttggacttgc
    22501 agaaattttt gaagccctag aacaactgag tcttagcaag cgatacaaaa acattgcctg
    22561 taaactaaag gaatcaattt gtaaacaaat gactatcgat ggtccttttg gtgagcaatt
    22621 tttggaggga atcggtgatg aggagaaaac aacctattca gtgaagcact atcaaaaacc
    22681 gattcttgag caagggttaa tctttttaag tgatgtgatt gtggatggaa aaattaattt
    22741 attgatgcat gatggagaag aatcagacac tacgttgatg cctttttatc aatttttaga
    22801 taataaagat catttatatc agaacaccat ggccttttct gctagttctt tcaatccaac
    22861 ctatagcgag gaaatcaaag gcatcacttg gggattagaa tccggcgcca cttttcctgg
    22921 ctttatcact gttttaatga gcgagctgca caaccctcaa gctttcgtaa cccgattaga
    22981 agaactaatt tgtttggcag atttagacgg ttcgtggtgg tggtggccgt atcgcttaga
    23041 ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc aaatgcggct gggcatcggg
    23101 tctttttgtc agcctaatga tcagtcaata tttcggattg aaattcaaaa aaggagtatt
    23161 gcaaattgat cctgttgata atttaacgtt ttcttggaag aatttgaagt tcggtcaagc
    23221 cttcatttcg attgaatgta cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc
    23281 attgaaaata agtgatacga aaaaaattct tcacgtagaa aagggaaaaa cgattcacat
    23341 tcaaaggaga aaaagatgag tatactaaaa aatattaccc aatttaaaaa aatccagagt
    23401 cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta aagctgcgat gttgccaagc
    23461 gagctaggac caagccgcaa aggtcgtggc gcaattccgc tgacttctgg tgaagaaact
    23521 gtgattgcgg atattactgg aacgggaata attcgtcata tgtggatgac gattcgtgat
    23581 cgaacagaat ttggcagttt tgttttacgt gatttaatct taagaattta ttgggatggg
    23641 tcggatagcc ctgctgttga aagtccttta ggggatttct tttgtaatgg gtttggagag
    23701 cgttgcgagg tgaactcgtt gccgatagtg gttaatccta cagggggctt taattcctat
    23761 ttcgaaatgc ctttccaaac atccgctaaa attacgatcg agaatttaca tccgaaggat
    23821 atgtcctcat ttttttatac gataaattac gctttggtgg acgaattgga agaaaatagc
    23881 ctatattttc acgcaaattg gcatcgggaa cgcattacca aattacagga ggattataag
    23941 ttaattgatc gattaaaggg tcaaggctat tacgtgggta cttaccttgc gcttacctgc
    24001 ctagaacgtt attggtgggg cgaaggcgag tttaaattct acattgatgg ggatagtgag
    24061 tatccgaccg taacctcaac aggttcagaa gattattttg gcggggcgtg ggcctttcat
    24121 gagtacgatg agttcggacg tccacatgcg aaaaactatt caacagcctt tttagggtat
    24181 ccttaccaat caaatagaga tgccacaaga gatcgttttg aaacgggaaa attgaatgcc
    24241 gttcatgcct ttggtgacga cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa
    24301 gaccccatct gttttaccga agatttatcg gtcactttac aacaaatcgg caacgatgat
    24361 gttcgcttat ttgagcggca agacgatgtt gctagtgtcg cttactggta ttcaacagaa
    24421 gcttcaggaa atactaaacg tcatttcact gatcgagaac gcttgccgcg ttaatgaagt
    24481 gtttttcaca tattcgaagg gaggaaccta tgatgatata tggggcaatt gaagctggag
    24541 ggactaaatt tgtctgtgca gtcggtactg aggaattaac gattttggaa agagtcagtt
    24601 ttcccacaac aactccagtt gaaacaatga agcaggtcat tgattttttt aaacctttta
    24661 aggaacaatt ggcaggcatc ggggtcggct gctttggtcc aattgatatt caatgcaaat
    24721 caacgagttt cggtcatatc acatcaacac caaaacttgc ttggcaaaat ttcaattttg
    24781 taggaacgct aaagcaatat ttcaatattc cgattgaatg gacaacagat gtcaatgcgg
    24841 cttgctatgg tgaatatgtt gctggttctg gcaaagggtt agcaggtgtt gcatacttca
    24901 ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg gactttcgtg gaagggttca
    24961 gtcatccgga aatggggcat atgttgatca agcgacatcc aaaggacaat ttttcaggaa
    25021 actgtccctt ccaccaagat tgtttagagg ggatggcagc aggaccagcg attgaaaaac
    25081 ggttgggtgt aaaaggccag aatttattgg ctgacgattc gttttggcag attgaagcct
    25141 tttaccttgc ccaatgtgca tacaatacaa cactgatgtt ttcgccagat agaattattt
    25201 ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa agttcaggat aaatttgttg
    25261 agcttataaa tggatatgtt gaaataccac caattgatag ctacattatt acgcccgaat
    25321 taggtgataa tgccggaata atcggcggat tagcattggc cagaaaagcg gtaagaaata
    25381 agcaaccata attaatgaat gtttcattga tactcatgtc aaaagtattt aaccgagtaa
    25441 tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt caaataaaaa ggctctaagt
    25501 caaatcgtgt tgttttcatt aaatcactga aaggaaatgc ttctgcgttt ccttttttct
    25561 ttttgttgtg cgctgtatac cttgaggata ttcactggtg gcgtacactt aaatagtaat
    25621 atattgtgtt actttatgtc tgtggtaata gttatagttg cttaccaatg tatgttcatt
    25681 tttcgaaatg ttgacttcat aatccttggc ttaatgtttg aatttttttg gtgatagatt
    25741 ttttcagccg tataaaataa atcgtaattc tattatagag agttgtgatt ataaaaagat
    25801 agcaagaaaa aaaaagaaag tttctataca ttttaaatat tttattccta tttctattgc
    25861 taaaaaagta gaaattctat atttaccttt aactctcgct tctcttgcaa atgtagttag
    25921 cattttaatc gataataaac
//
LOCUS       DAYYKC010000034        25633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000034 DAYYKC010000000
VERSION     DAYYKC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.034"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..836
                     /locus_tag="ACHBBW_001349"
     CDS             282..836
                     /locus_tag="ACHBBW_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153041.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1009..1482)
                     /locus_tag="ACHBBW_001350"
     CDS             complement(1009..1482)
                     /locus_tag="ACHBBW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153042.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1494..2441
                     /locus_tag="ACHBBW_001351"
                     /pseudo
     CDS             1494..2441
                     /locus_tag="ACHBBW_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2712..3147
                     /locus_tag="ACHBBW_001352"
                     /pseudo
     CDS             2712..3147
                     /locus_tag="ACHBBW_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3163..3996
                     /locus_tag="ACHBBW_001353"
     CDS             3163..3996
                     /locus_tag="ACHBBW_001353"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153043.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4241..5266
                     /locus_tag="ACHBBW_001354"
     CDS             4241..5266
                     /locus_tag="ACHBBW_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153044.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5404..5529
                     /locus_tag="ACHBBW_001355"
     CDS             5404..5529
                     /locus_tag="ACHBBW_001355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153045.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5554..6669
                     /locus_tag="ACHBBW_001356"
                     /pseudo
     CDS             5554..6669
                     /locus_tag="ACHBBW_001356"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6752..7648
                     /locus_tag="ACHBBW_001357"
                     /pseudo
     CDS             6752..7648
                     /locus_tag="ACHBBW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7664..8488
                     /locus_tag="ACHBBW_001358"
     CDS             7664..8488
                     /locus_tag="ACHBBW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153046.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8520..9830
                     /locus_tag="ACHBBW_001359"
     CDS             8520..9830
                     /locus_tag="ACHBBW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9153047.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9978..11237
                     /locus_tag="ACHBBW_001360"
     CDS             9978..11237
                     /locus_tag="ACHBBW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9153048.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11247..12119
                     /locus_tag="ACHBBW_001361"
     CDS             11247..12119
                     /locus_tag="ACHBBW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153049.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12131..12961
                     /locus_tag="ACHBBW_001362"
     CDS             12131..12961
                     /locus_tag="ACHBBW_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153050.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13001..15184
                     /locus_tag="ACHBBW_001363"
     CDS             13001..15184
                     /locus_tag="ACHBBW_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9153051.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15245..16465
                     /locus_tag="ACHBBW_001364"
     CDS             15245..16465
                     /locus_tag="ACHBBW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9153052.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16462..17922
                     /gene="gtfA"
                     /locus_tag="ACHBBW_001365"
     CDS             16462..17922
                     /gene="gtfA"
                     /locus_tag="ACHBBW_001365"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9153053.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17999..19105
                     /locus_tag="ACHBBW_001366"
                     /pseudo
     CDS             17999..19105
                     /locus_tag="ACHBBW_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19427..19636
                     /locus_tag="ACHBBW_001367"
     CDS             19427..19636
                     /locus_tag="ACHBBW_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9153054.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19629..19778
                     /locus_tag="ACHBBW_001368"
     CDS             19629..19778
                     /locus_tag="ACHBBW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9153055.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19932..20597
                     /locus_tag="ACHBBW_001369"
     CDS             19932..20597
                     /locus_tag="ACHBBW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153056.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20881..21966)
                     /gene="xerS"
                     /locus_tag="ACHBBW_001370"
     CDS             complement(20881..21966)
                     /gene="xerS"
                     /locus_tag="ACHBBW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9153057.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22300..22533
                     /locus_tag="ACHBBW_001371"
     CDS             22300..22533
                     /locus_tag="ACHBBW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9153058.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22530..22937
                     /locus_tag="ACHBBW_001372"
     CDS             22530..22937
                     /locus_tag="ACHBBW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9153059.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24074..24592)
                     /locus_tag="ACHBBW_001373"
     CDS             complement(24074..24592)
                     /locus_tag="ACHBBW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9153060.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24959..25207
                     /locus_tag="ACHBBW_001374"
     CDS             24959..25207
                     /locus_tag="ACHBBW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153061.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8731 a         3913 c         4772 g         8217 t
ORIGIN      
        1 aaatttcata taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc
       61 ccgtctaaaa tttaaataag agcttagaat cttcatataa gtaaggttaa tttttttgta
      121 caaaaaagtc ggaatattct gagttactga aatgatataa aatcataaac taaatccctt
      181 gattttattg actttcaaaa gtttatatat ataagttaat gattatggta aaatatatac
      241 ataatcatta actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac
      301 cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc
      361 cggaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata
      421 ttataggatt aaaagttgga gatatacgag gcaaaagtaa gcctattatt gttgaacata
      481 aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagaaatt ttattatata
      541 ctgaaggtaa agaagaaaat gattggttat ttccgagtcg gcaacaaggt cgacatataa
      601 caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga cgagatgata
      661 taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca
      721 ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg
      781 gtataactga agatgaaata ggagcatcac ttcgtggatt taaactaggt gtataattta
      841 aaaattttta taaacagtaa agacaaagat taaaagctat ttttatctag aacgatttta
      901 tttcataaag ttcgcggaat aaaagctggg gttactggta cttggtatag gttcgtgtta
      961 tataaactaa gtaattaaat aacattttta ggttggaaaa ggatgaaatt aaaaaaagag
     1021 aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg ataactcaac
     1081 gtttagcaga ctatctaata gggatagtct gcttttttcg tttttgacca tacttttgat
     1141 cttctgcaaa cttccttgct tttcctctgt agaactcatg aaatctgcaa aacgaattgc
     1201 cgtttcctct gtctgctttt tcgcgatatg ggtgtatata tcatccatag tggttttaat
     1261 atctgtatgt ccaagtcgaa catacacttc tttgacagac gcaccagatt caaagagcag
     1321 actacaatgg gtatgacgaa aaccatgaag tgtgatatgt tttaagttat tctctttaat
     1381 gatgattttc aaattgtgat ttacatagtc taaataaagg ggcttatttt cttctgatga
     1441 aaacaaaagt tgttcaccat cttttatagg tttgattctc atttttagat attatgcccg
     1501 cacgtattca tcacatttct attgtgaatc gctttattcg accgacattt gacttttatc
     1561 ataatatact cggtttaaaa ttactaatga aaacaattaa tcaagacgat cataccatgt
     1621 atcacctgtt tttttcagat aatcaccacc gtgtgggaac tgaactgact ttttttgaag
     1681 tacaagaagg gaacaaccag ttttttggaa ccaatacgat tgaacgaacg atactaaaag
     1741 ttccttccga agcatcattg catttttggg aaatatattt tgaaacacaa ggcgtttgcc
     1801 attatggaat cgaatcattt tctggacgac ctatcttacg ttttgaaggg ccagatgcaa
     1861 cacgattagc tttagttccc ttacgcgaat ttgaagatat tgatgattat ttcccgtatg
     1921 tcacagacca gatccctaca gagcatgcca ttttaggaat tgatgccatt caactacgcg
     1981 tacaatattc cgatgcgact gaacgagagt tattgtcggt aggctggtag cacaaagaat
     2041 acgtcagttt cttttcgggt catcaacgag tgacaatctt agaaaatcaa gacactcagt
     2101 tctatcaaga agttcagatt attcaagatc gtgagaatcc agttgccatt gaagggattg
     2161 gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc tgatttacaa cgcatcgaca
     2221 aacaattaca agagcgcaat tttataaatt caggaataaa agatcgtgaa ttttttgtat
     2281 cactttatta tcgtgaccca aatcaattat tgattgaaat tgctacaggg gaaggaaacc
     2341 tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga agaaattcct ttatttttac
     2401 cgcagtactt aaatttcatt cgtgaacaag tggagcaata attagctaat caaatccatg
     2461 acgaagagta agaggttgac tcataagtca aaaaataaag aaaaacgcat caaatccatt
     2521 ctaaagaatg ttgatttgat gcggttccta tttttataaa ctatcatttt cgactaataa
     2581 aactacttat gagtcagcct ctcttttttt cttgattttt atacctcttt ctaggtaaac
     2641 taaacatctg ccaaaaatac cgtgtctcat tgaaacattc tcaactattt caaaataaag
     2701 gggagtacca aatgaacgag tatattgaaa ttatccacgc aagagaaaca acttaaaaat
     2761 gtcagtctca aaattcccaa aaataaaatt agtattttta ccagcgtatc tggttcagga
     2821 aaatcatcga ttgtttttga tactattacc caagaagctg gtcgtcaact gaatgacacc
     2881 tatgatagtt ttacccgttt atttttacct aaatacagtc gtccagaggt tgatgagatt
     2941 gataatcttt ctacggcgat tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca
     3001 acgttaggga cgattactaa catcaatgcg ttatttcgtg tgttgttttc atgttttgca
     3061 acacctagtt taggatatgc aaatgcttat tcatttaatg atcctagtgg aatgtgttct
     3121 gtctgtcagg gaatcggtaa gacgattgca tttttaatag gattgcttta tttttcggct
     3181 attgataatg ctgattttgt attttctgat ccatttaccg cagaagtttt gtctggaatt
     3241 gaggaacagg caaagaatca tggttatttt gtattggtgc ataatgttaa ttcagcagaa
     3301 gatgttaaaa ctattcagag aaattggcgt tttgaggggt tcattgttgt tggtgtacgt
     3361 gtaaaagaat ttttggaatt aaataacgtt tttgatggtc ctgttgttta tattgatacg
     3421 catctttcaa aagagacatt aagagaactt gaacaacaaa tgaatcgttt atttgtaaat
     3481 acaaatgatt accaagctag tacattggca gtggaacatt tgattcatta tggtcatgag
     3541 aaaatagcgt ttctttcttt tgactacatt gtaggtgaac caagtgttat tcaagaacga
     3601 catcaagctt atatcgatac attaaagaaa tatcatttaa cctttaaatc gcattggata
     3661 tataacaact ctgaatttga gcgaatttat caagaattag ctgaatttac tgcaattgtt
     3721 gttactggag attatttagc ggctaaatta gtaaaatttt taaaaaataa gaataaacga
     3781 gtgatggaac aattatcgat tattggattt gatgatatta cttttgcaga attaatggat
     3841 cctgcgttga caacaatacg actaaatcca ggtaacaagg gggcgattgc ttttcaccaa
     3901 ctgattggac tggccaaaca agaagaaata aaaaatcaag tactactatt agagggagaa
     3961 ttgatttctc gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag
     4021 ttaacaataa aatatccgaa cgatagagag aatgaactgt gatctagcag ttcctcgtcg
     4081 caaattcact agaattagca ttatttactc taaattgttc ggatattttt tcttgttttt
     4141 aattatgatt acagtgccta tttttaacga aatttgctta ttttcaaaat gtatgattat
     4201 atgttagtaa acactttcaa atatgaaaag aggagctatt atggtaggaa ttagggatgt
     4261 tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg aggatccgac
     4321 actttcagta accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt tttatcatta
     4381 tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt gcattaataa cgacagtttc
     4441 tgaagtagat gagttggaag atccctattt tcgaacgatt cgcagaggga ttcaagtcga
     4501 agcagaacaa agcaaagtca atttgaagaa aattattcgt ttgtcagaaa caccgttgga
     4561 gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt
     4621 gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc ctagtattga
     4681 agctgaagta gatgcagttt atactgattt aacaaaagca acgaatcagc atttagatcg
     4741 attgtataaa aaaggacacc gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga
     4801 tagccaagga aataaacacg tgtcagatga tgattatcga aaaatagcct atgagacgtg
     4861 gatgaaacaa aaaggattag aaaaatatag tcaaatttat ttagaaggct ggaccacact
     4921 tgacggaatg caggcagcag atttactttt gaaaaaacat gctaaagata agttgccgac
     4981 tgctatcatg gtaggaaatg atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa
     5041 tggtctacat attccagaag atatttcgat tgtgagtttt gataatattg aagttgctga
     5101 atttttaaca cctgcattaa atacggtgaa tgtgaataca gaagaaattg gaaaactagc
     5161 agttcgcatg gttaatgaac gaataaaaga agttagaaat gtttcgatca aagtgattgt
     5221 ttctaataat attattgtta gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc
     5281 aaaaaagggt gtttactttt ttgtttacta atatttttag taaaaaatct cgacaaaaca
     5341 ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt
     5401 aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac tgggacatta
     5461 ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac tttaagctat
     5521 gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga atttgaaaaa
     5581 caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata ttgggttaaa
     5641 ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca ctctagcgaa
     5701 tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat caaagaaaat
     5761 gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct tgaagacgaa
     5821 ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa agaattattt
     5881 gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact cagacaagct
     5941 gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca tattggaaca
     6001 gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc cgatgacaat
     6061 acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta tcgagatatt
     6121 gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga taagttaagt
     6181 cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc accgggattt
     6241 ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt
     6301 aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa atacccagaa
     6361 gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat ccaagcggaa
     6421 acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc ttcttcagat
     6481 acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg tccatacaat
     6541 aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat tctgtctggt
     6601 gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc gatattgagc
     6661 ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt cgtctgtata
     6721 ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg gaaaatatcg
     6781 taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag gattaatttt
     6841 attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt
     6901 cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt
     6961 ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta gtattgttgt
     7021 attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg
     7081 gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga tttggcgctg
     7141 gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag gtctcgatgg
     7201 accagcatgg ttaacggatc catcgattgc gatttattct attatcattg taggagtttg
     7261 gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa ttcctaaaga
     7321 ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt tcaacattac
     7381 gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta tcaatgcttt
     7441 ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca tgtttaaatc
     7501 acaatcatta gcctatttat tctataaaca ttcatttatt ttaaatgaca agggatatgg
     7561 ttcggcaatc gttatgttcc ttttagtgat tatattaatt attactgcaa ttcaaatgaa
     7621 gttgcagaaa aagtgggtaa actattaagg aggctatcaa attatgaaaa ataaattttt
     7681 ttcaaatgcg ccaatccata tcatgcttat tttaggtggt attgcaatgg cattaccttt
     7741 tatttggatg attttaactt cagggaaaac gttggttgaa tctacccaaa ttccacctac
     7801 aattttccct gagaaatttc agctaagtaa ctatcaacaa gtacttgatt tattaccatt
     7861 catggctttt tatatcaata catttttaat ggttgttgga cgagtgattg gctcggtttt
     7921 attttcagca atggctgcat atgcttgtgc gcgtctaagt tttccaggga aaaatatcct
     7981 tttcagctta gtacttattc aaatgatgat tcccggttcc atttttatta ctccacagta
     8041 tttattagca caaaaattgg gtattttaaa tacaattcca gcattaatat taccaggggt
     8101 agttagtgcg tttggtacgt ttttattaag acaatttttc ttaggtttac ctgatgaatt
     8161 ggaggaagcg gcctatttag aaggtgccaa tatttggacg atctttttca aaattatgtt
     8221 gccgcttgct aaatcaggat tggttgcact tggaatattc acagcgttgt ttgcatttaa
     8281 agatttaatg tggccattga ttgtcaatac ttcaattgat aagatgcctt tagctccggg
     8341 attagcaaac ttacaaggac aatatgccac gaacttccca caattaatgg caggttcgtt
     8401 attagcaatt tggccaatag ttgtgatatt catcgttttc caaagaaaat tcattgaagg
     8461 aattgctaca acaggtagta aattataaaa ctattaatta gtgattggag agtaagtaca
     8521 tgacaaaaat tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg
     8581 atgcgatgtt aactccaagt ctacaagatg cacatattgc attgtacgat attgatgaaa
     8641 aacgactaaa agaatcaaat ttaatgctta aaaatattaa taaaaattcg aatgaaaatc
     8701 gtgctgatat tgtttcttat tatgatcgca aagaagcgtt aagagaggct gattttgtta
     8761 tcaatgctat tcaagtgggt ggttataaac cttcaacagt gattgatttt gatattccta
     8821 aaaaatatgg gttgcgacaa actattggtg acaccatggg gattggtggg atttttagaa
     8881 ccctacggac actaccagta atgttagact ttgctaaaga aatggaagaa gtttgtccaa
     8941 atgcttggtt attgaactat acaaatccga tggcgatgtt aaccatgggt gttttaaaag
     9001 cgacgagtat taagactgtt ggtttatgtc atagcgttca agtatgcgta ccagaattgt
     9061 ttgagcattt aggaatagcc gatcaatatg acttaaatga ctttcaatgg aaaattgcag
     9121 gcattaatca tatggcattc ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa
     9181 caattcgtca gttagctgca gaaaaaccaa atccacatcc agattctgta cgttttgaat
     9241 tgttgaaacg ttttggttac tatattacag aatcaagtga acacaatgca gaataccatc
     9301 catactttat taaatcaaaa tacccagaat tgattgacga attagggatt ccgttggatg
     9361 aatacttacg tcgttgtgaa caacaaatta atgattggga aagcatgcga gaggacattg
     9421 taaataatgg ggagttaaca catacgagaa gccgtgaata tgcttcatat atcatggatt
     9481 ctattacgac aggaactccg tcagtgattg caggaaatgt cttaaataaa ggcttaatca
     9541 caaacttacc gacagatgtt tgtgtggaag taccttgttt agtcgataaa aatggcgtgc
     9601 aaccaaccta tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg acgaatatca
     9661 atgttcaaga actgacagta gaagccgctt taacactaga taaatcaaaa atttatcaag
     9721 cagcgtattt agatccacat ctatcatctg aattgtcttt aacagatatc aaaaatttag
     9781 tggatgattt aatcgaagca catggggact atttacctga atataattaa aaagaagctg
     9841 aaagagtaag gaattttcaa ttccttactc ttttcttagt taaatttagt aaaaatattg
     9901 acaaaacact tagtaaacag ttacattagt gttaacaaaa agaaaacgca ttcaaattgt
     9961 ataaggagga gcataaaatg aagtggtcta aaaaaattat tttagcttgt atagcactag
    10021 gagcaacgat ctctttgact gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag
    10081 aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag attgctaaag
    10141 attttgaagc aaaaaaccct gatattcatg taaaagttgt ggatgtaccg aatgctggtg
    10201 aagtaattaa aacacgtatg cttgcaggcg atgtaccaga tgtcattaat ctttatcctc
    10261 aaagtataga attgaaagaa tgggcgaaag ctggttattt agaggactta accgaagaac
    10321 cgtatttaga gaatatcaaa aatggatatg cacaacgttt tgctattgag gatcgtgtat
    10381 atagcatacc attaaccgct aatgtctatg gtttttacta taacaaaact gcttttgagg
    10441 aaatggggat tcaagcacca gaaacttggg cagaatttga aaaaatagta gctgagatta
    10501 aagatcaaaa caaagtaccg tttgcgattg caggagcaga aggctggact ttaaatggtt
    10561 atcaccaact agctatagcg actgctgctg gtggtgaaga ggaagcgaac aatgtttggc
    10621 gtttctcaga agttaatgga attaatgctg aaagcaaaga aatgcaaaaa gattttaatc
    10681 gattagattt gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata
    10741 acgatacagt agttactttt acaaaaggtg atgcgttgat tatgccgaat ggttcatggg
    10801 caatgccgat gattcattca caaaatccag attttgaagt aggaactttt ccttttccag
    10861 cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga cctagcctta tcagtttctg
    10921 caacttctca acataaagaa gaagccaaac gttttgtaga atatatgaca acacctgaag
    10981 cgatgcagaa atattatgat gtagatggtg caccgtgtgc tgttgagggc gttatcgaga
    11041 atactcagga ttcaccatta agtggtttaa ctgaacttgc ttttactgat cgtcatttag
    11101 tttggttagc caaagattgg aatagtgaaa atgattttta taccttaaca acaaattatt
    11161 tacacaatgg caatcaaaaa gccatgatta acgcattaaa tgctttcttt aatccaatga
    11221 aagctgacgg aaggtaggaa aataaaatga aaataaaaga aattttaaat cgttactggg
    11281 caatcatttt tgtagcaatt ccaattattt tgcagatgat ttttttctac tttccaatgg
    11341 ttcaaggtgc tttttacagt ttaactaatt ggactggtct gacttataat tttgattttg
    11401 tgggtttgaa caattataag attctttttt ctgatccgaa gtttatcaaa tcgattggct
    11461 tcactttaat tgtgacgctt tgcatgattg ctggacaaat tggttttggt attttgattg
    11521 cgcgggcgtt gaacgcaaat attaaaggga aaacattctt tagagcatgg ttcttttttc
    11581 cagcggtctt atcgggcgtg gttgtttctt tagtatttaa acaaattttt aactatggtt
    11641 taccaaaaat tggtagtatg ttaggcattg aatttttaca aaccagtctt ttaggaacaa
    11701 catggggagc gatttttgca gtaatttttg ttttactttg gcaaggaatt gccatgccag
    11761 tcatcatttt cttagcgggt tttcaaagta ttccacaaga aattattgaa gctgctagaa
    11821 tggatggggc gaatgataag caaatttttt ggaatattga aatcacctac ttattaccaa
    11881 gtatttcaat ggtatttatt atggcattaa aaggtggctt aacagcattt gatcaaattt
    11941 ttgctttaac tggtggtgga ccaagcgatg ccacaacatc tttggggtta cttgtttata
    12001 attatgcttt taaaaacaat cagtttggtt atgcgaatgc tattgcttta gtcttattta
    12061 tcttaattgg gattgtttca ctggcacaat taaaattatc gaaaaaattt gaaatctagg
    12121 aggtttccca atgaaaacat caaaagaaaa aaatatttct aaatacttgc ttttattatt
    12181 agggggcttc atgattttag ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac
    12241 acccgatatt atgaataatt tctttcgttt tcctaatcca gcgactttgg aaaatttccg
    12301 aagattgtta gcggatggaa ttggtcatta cttttggaat tcggcaatca tcacaatcat
    12361 tgcagtagtt gctgtgacag caattattcc tatggctgct tattcactag caagaaaaat
    12421 gactcgtggg aaagcatacg gattgatgta tttattttta attttaggaa tttttgttcc
    12481 tttccaagta attatgattc cgattaccgt tatgatgagt aaaattggtt tagcaaatat
    12541 gtggggatta atcttactct atctcactta tgccgtacca cagacactat ttttatatgt
    12601 tggttttatt aaactaaatg tgcctatcag tctggatgag gctgctgaaa ttgatggggc
    12661 tagtcacttt acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac
    12721 gactattatt cttaatgctc tatggttttg gaatgatttc atgctaccat tattaatttt
    12781 aaatcgtgat tctaatatgt ggacactacc attgtttcaa tataattata cgggacaata
    12841 ttttaacgac tatggtccaa gttttgcttc ctatacagta ggaattgtca cgattacact
    12901 agtttatctt gctttccaaa gaagtattat agctggaatg agcaatggag cagtgaaata
    12961 accaataaat tattctcatt ataaaggagt tcataaactt atgattatat ttgacaatca
    13021 cacacaaaca tttcatttac aaaataaaaa aattagctat ctattaggaa tcgaagaaaa
    13081 taattattta acgcatcttt attttggaaa aaagattacg cactattctg gtgggtatcg
    13141 ctacccccga acagatcgtt ccttctcacc caacaatgca aatgcgcaag atcgtttatt
    13201 atctttagac acattaccat tagaatttcc aggatatgga catggtgatt ttagagaacc
    13261 ggcgcataat attaaattag gaaacggtag tcgtgtaaat gattttcgtt atgatagcta
    13321 cgaaattcaa aaaggaaagc ccagcttagc gggattgcca gccttatata cggaagatga
    13381 tgatgaagct gaaacgctga ttattcaatt gaaagacgcg attagtggat tgactttaca
    13441 gttgttctac acaatttatc aaaatcacag tgtaattatt cgttctacgc aattagtaaa
    13501 taatagtgat gaaacagtgg agattaaccg cttagctagt caatcaattg attttccaaa
    13561 tcgtccactc gagttaattc atttaaatgg tacctgggca agagaacgtc aaatgactag
    13621 agaaaaaatt catacaggga taaaagtatt agatagtaaa cgcggatcaa gcagccatca
    13681 acaaaatccg tttgttgctt tagtagatgc caaaacagat gaatttcaag gagaggcata
    13741 tggatttaat ttagtttata gcggtaatca cgaaacggtg attcaaaaag atccatttga
    13801 ccaaacacgg gtcattatgg gaattaattc atttaatttt ggttggcaac taaaaactgg
    13861 cgactcattt caaacgccag aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt
    13921 atcacagacg tatcatgaat tgtataatca ccgtttaatt cgtggaaact atcgtttaaa
    13981 agaacgtccc acgttaatca acaactggga agcaacgtat tttgatttta acgaagaaaa
    14041 aatcatgaat attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg ttttagatga
    14101 tggttggttt ggcggtcgtg aaaatgactt cacttcatta ggtgattggg ttgaaacaga
    14161 aggcaaactt gcttgtggtt tggaaaattt agccaagcaa gtgcatgata acgggatgaa
    14221 atttgggatt tggtttgaac cggaaatgat ttctgaagat agtgaactat ttcgagcaca
    14281 tccagattgg gcattgggta ttccaggtcg tgaccgctca attagtcgaa atcaatatgt
    14341 tcttgatttt tcaagacaag atgttcgtga taatatctat caacaaatga cggcgatttt
    14401 tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat cgaaatatga cagaagtcta
    14461 ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct catcgttata tgttagggtt
    14521 atatgaattt ttagaaaagt taacaacgaa ttatccaact attttatttg aaagttgttc
    14581 gggtggaggc ggtcgttttg atgcaggttt cttgtattac atgccacaaa cttggacgag
    14641 cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc
    14701 aatttcaagt atgggtgcgc atgtttcagc agtcccaaat catcaaacag gaagagaaac
    14761 gagtttaact attcgtggca atgctgcgat ggctggtgtt tttggttatg agctagatct
    14821 aacgcaatta acttctgaag aaaaagcaga aattaaagaa caagttgcct tttataaaaa
    14881 gcatcgccaa cttttgcaat atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa
    14941 tgatactgca tggatgtttg tctcaaaaga ccaaacagaa gccatggtct tttatttccg
    15001 agtattagca gaagctgcgc atccattagt cacattgaaa ttagcaggat tagcagaaga
    15061 agcaacttat caaatggaag atgtaacatt tgctggcgat gaattgatga atctcggttt
    15121 ttatattgac tcagaactac atggtgatta tgcgacacaa cgcttttatt tgaaacagat
    15181 atagtagctt gaatttacaa tataagtgca acaccaaaag agactcattc caaacgaaga
    15241 aaatatgttt tatttacacg catttactaa aaagcagcct gatttgaatt gggaaaatga
    15301 agaagtacgt gaagaaatct atgcaatgat taactattgg ttagataaag gactaggcgg
    15361 atttagaatc gatgcgatat taaacatcaa aaaaaaacta gagtatggca tctttgaatc
    15421 tgatggagaa gatgggttag ccttcattgg gccctggatt cttaatcagt cgggcattga
    15481 agtgtggtta aaagaaatga gagagcggac atttaaacca cataatagta tgacggtagc
    15541 agaagccgat gttcctaatg aacgtttaga tgaatatatt ggtgaagatg gctattattc
    15601 gatggttttt gattttagtt atacggatat tgatgtacct gaaactggtg aatggtttaa
    15661 agatagccaa tggacatgga cggatatgcg taccaatatt tttacgaatc aattagttac
    15721 gcaagataaa ggttggggcg ccttatattt agaaaaccac gatcaaccac gttcaattaa
    15781 taaatatatt ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt
    15841 tatgttacta agaggtaccc cttttattta tcaaggacaa gaactaggaa tgaccaacat
    15901 agaaatggca tcattagaag attttgatga tgtagctacg cattctcagt ataaacgggc
    15961 attagaatat ggattggcac cagagcaagc attgaaagct gtcaataaac gaagtcggga
    16021 taattctcgc acacctatgc aatggaatac gcaaaaaaat gccagattct caacttctga
    16081 taatgtatgg ttaaaagtaa atcctaatta tccagaactc aatgcagaag ctcagttaac
    16141 caatcaacag tcggtattca attattatca tcaattaatt aaattaagaa aaaatagtgt
    16201 atacaaagac gtgctaatct acggacaatt tctaccagtg aaagataaaa atgaacatat
    16261 tttgctttat gaacgccaat tagaagataa aatcgtatta gttatcttaa acgttacagc
    16321 aacagaacaa gaatattatg tagatgaaca gtaccaacaa gtattaatca ataattatga
    16381 agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt
    16441 agctaattat ggaggacaat tatgaaaatt aaaaacgaag caatgctcat cacctatgca
    16501 gatagtttag gaaaaaacat gggcgaatta aaacaagtct tagacaaaca tttacaaggt
    16561 gttattggtg gtgtacatct gttgcctttt tttccatcga caggtgatcg aggctttgcg
    16621 ccgagtgatt atacgacagt tgatccagct cttggaacat gggaagatgt ggaagcatta
    16681 ggtgaagatt attatttgat gtttgatttt atgattaatc acatttctcg tgaatcaaaa
    16741 tttttccaag atttcaaaaa aaaccatgag cagtcaccat acaaagaaat gtttatccgt
    16801 attcatgaat ttttcccaga aaatcgacca acacaagaag atattgattt aatctacaag
    16861 cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg atggtacgac agaagaggtt
    16921 tggaatacct ttggggaaga acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa
    16981 tttattcgag aaaccatcaa agatatggcg aaccatggct gttctttaat tcgattagat
    17041 gcgtttgctt atgcgattaa aaaattagat accaatgatt tcttcgtgga accagatatt
    17101 tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga attattacca
    17161 gaaatccatg agcattattc cattcaaatg aaaattgcca atcatgatta ttatatctat
    17221 gacttcgcct tgccaatggt cactctgtat tcactttata gtggaaaatc agaacgttta
    17281 gccaattggt tgaaaatgag tccgatgaag cagtttacaa cattggatac gcatgatgga
    17341 attggtgttg tggacgctcg tgatttatta acagatgaag aattggatta cacctctgag
    17401 gaattgtata aagtcggggc gaatgtgaag aaggtctatt caagtgccaa ttacaataat
    17461 ttagatattt atcaaattaa cagcacctat tatagtgcat taggcgataa tgaccgtagc
    17521 tatctgttag cacgtgcgat tcaagtattt gcgccaggga ttccacaaat ttattacgtt
    17581 gggttattag caggaaaaaa tgatattgaa ctgttggaag caacgaaaga agggcgtaat
    17641 attaatcgtc attactatga tttagccgaa attgaagaag aagtgcaacg gccagtgatt
    17701 caacaattat ttgcactttt aaaatttaga aatgaatctg cggcgtttga tttggatgga
    17761 acgattgaag tagcaacgcc atcagaatca gaaatagtga ttacacgtaa aaataaaaac
    17821 ggtagtgata gtgcaacatt aacggctaac ttggatacga aagaatttat cattgagtct
    17881 aaagatataa aaattttcac tagtgttgca ttaaaatttt aacctattcc atagaacaaa
    17941 aaatccttta tactagcact tggatgactt atccaagacc aataaaaagg actcatacat
    18001 ggataagtat aaaacaaata cagcatttaa taaatggttt tctgccataa aattaggaaa
    18061 actttctgtc aactcccaaa aatgtatttt tgattacaat tcgtatcgta aaaaactgtc
    18121 ttttgaagct gtgctcaagc tctttctcta cgcagttaat gaagaaaaag aaagtttgcg
    18181 tgatttgagt acagctctta tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat
    18241 tagtcatacc caattgtctc gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat
    18301 ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct
    18361 gtatctgatt gattccagta cgttttcgct tagtttaaaa cgtcataagt gggcgacttt
    18421 ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat tgttatatgg acgatactac
    18481 gatgtatcca actgatttta ccttaacaaa tgcccatgaa catgatctca atcaactacc
    18541 agtattggtg aatcaaccag aggcaactta cgtgtttgat cgtgggtatt aggactttga
    18601 aaagatggat cagatgcatt gggatggcta tttcttcgtc actcggatta aaaaaaacac
    18661 caaagttcac gtgatagata ctctggaaac atcgccagaa acagaaattc tacgagacga
    18721 gctggttcgt ttaggatcta aaacctatct aaccgccaat ttccgactag tcactgttca
    18781 agataagaac ggacgtgtgt ttcaatttat caccaatcga atggacgtct cttcaaaaga
    18841 aatctctgat atgtatcacg ctcgttggca aatcgaattg tttttcaagc acatcaaaca
    18901 acacatgacc attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat
    18961 tttaacgatg atttccgctc tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa
    19021 atcagtgttc caaatcaaac gattctttcg ttatttactg tttcagccct ttgaatgttg
    19081 tctagaaaaa ttaattccca catgagaaaa aagaaatttg gctcgttact tttggttttc
    19141 agttgaataa cagataaaaa attttctgga aaaagtcatc ctctgcgtca gcacagtttt
    19201 ttttgactta ttttcagagg aaatagtttt cagaatattc taaaatgttt tatgcaacac
    19261 tagtgatatt ttttagaatt agatttctaa tgtgcatttt ttgagtatta aggagcaagt
    19321 atacaaagat ttataaagct gattaaattt gaataaaaat atgcaggaac gctactattt
    19381 ttagtagtgt tcttttttat atttgaataa aaaatgtggt ggttaaatgg tggttataac
    19441 aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca
    19501 ggcaaaagca attatggctt caaaaagcta tacctattac gaaaatagaa gattaggact
    19561 agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt
    19621 tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt
    19681 tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt
    19741 taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact
    19801 tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa
    19861 gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt
    19921 atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata
    19981 atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt
    20041 cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa
    20101 atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta
    20161 tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac
    20221 tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt
    20281 ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg
    20341 agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa
    20401 ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac
    20461 tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg
    20521 caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt
    20581 tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg
    20641 tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa
    20701 atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg
    20761 ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa
    20821 actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac
    20881 tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt
    20941 tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg
    21001 gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc
    21061 ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc
    21121 ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg
    21181 aatagctaga taattagcta aatattccat tgcgaatgct gctatattga cagcatctct
    21241 tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt
    21301 aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag
    21361 gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata
    21421 ttcattggca atataatcta gataattttg tgtatcctta tctagaaata attttttctc
    21481 aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat
    21541 aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt
    21601 aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt
    21661 attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg
    21721 tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat
    21781 agaagtatca ggaacgatag actcactcag aagccattca taaaagagct tataattttt
    21841 tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc
    21901 ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt
    21961 tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata attggactat
    22021 accaattata gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa
    22081 aaagcatata aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga
    22141 aggaaataga agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga
    22201 acaatctttc tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg
    22261 tatatactgt atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa
    22321 tttagggaag tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat
    22381 gggttaaatc ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca
    22441 aaaaaacaag ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat
    22501 gatactactt ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata
    22561 ttataaaaat gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa
    22621 aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag
    22681 aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac
    22741 atccttttca caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa
    22801 tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg
    22861 ctacgtatga taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac
    22921 atacgcagcc catttaagga gttactgtat gtgtctttat agggttatta gctgctatag
    22981 cgaaataatt gaagtaaatt acaaatagct ataattcaaa tccaactata tagaaaacgt
    23041 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
    23101 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
    23161 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
    23221 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
    23281 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
    23341 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
    23401 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
    23461 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
    23521 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
    23581 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
    23641 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
    23701 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
    23761 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
    23821 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
    23881 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
    23941 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
    24001 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
    24061 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
    24121 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
    24181 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
    24241 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
    24301 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
    24361 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
    24421 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
    24481 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
    24541 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
    24601 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
    24661 tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta
    24721 taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata
    24781 cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata
    24841 tcaaaatcat gttaactact atcaattact aataaaaact accactttaa gcgttaagaa
    24901 ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt
    24961 ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt
    25021 gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat
    25081 gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt
    25141 gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc
    25201 tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt
    25261 ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat
    25321 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
    25381 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
    25441 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
    25501 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
    25561 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
    25621 acccccactt agc
//
LOCUS       DAYYKC010000035        24927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000035 DAYYKC010000000
VERSION     DAYYKC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.035"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..695)
                     /locus_tag="ACHBBW_001375"
     CDS             complement(84..695)
                     /locus_tag="ACHBBW_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9153062.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(818..943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1025..1741)
                     /locus_tag="ACHBBW_001376"
     CDS             complement(1025..1741)
                     /locus_tag="ACHBBW_001376"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9153063.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1747..2316)
                     /gene="scpB"
                     /locus_tag="ACHBBW_001377"
     CDS             complement(1747..2316)
                     /gene="scpB"
                     /locus_tag="ACHBBW_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9153064.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2300..3070)
                     /locus_tag="ACHBBW_001378"
     CDS             complement(2300..3070)
                     /locus_tag="ACHBBW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9153065.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3067..3438)
                     /locus_tag="ACHBBW_001379"
     CDS             complement(3067..3438)
                     /locus_tag="ACHBBW_001379"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153066.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3457..4344)
                     /gene="xerD"
                     /locus_tag="ACHBBW_001380"
     CDS             complement(3457..4344)
                     /gene="xerD"
                     /locus_tag="ACHBBW_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9153067.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4440..4913)
                     /locus_tag="ACHBBW_001381"
     CDS             complement(4440..4913)
                     /locus_tag="ACHBBW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9153068.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5018..5881)
                     /locus_tag="ACHBBW_001382"
     CDS             complement(5018..5881)
                     /locus_tag="ACHBBW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9153069.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6077..8176)
                     /locus_tag="ACHBBW_001383"
     CDS             complement(6077..8176)
                     /locus_tag="ACHBBW_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9153070.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8396..9505)
                     /gene="rpoD"
                     /locus_tag="ACHBBW_001384"
     CDS             complement(8396..9505)
                     /gene="rpoD"
                     /locus_tag="ACHBBW_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9153071.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9529..11370)
                     /gene="dnaG"
                     /locus_tag="ACHBBW_001385"
     CDS             complement(9529..11370)
                     /gene="dnaG"
                     /locus_tag="ACHBBW_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9153072.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11552..13867)
                     /locus_tag="ACHBBW_001386"
     CDS             complement(11552..13867)
                     /locus_tag="ACHBBW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9153073.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14052..14912)
                     /locus_tag="ACHBBW_001387"
     CDS             complement(14052..14912)
                     /locus_tag="ACHBBW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9153074.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14971..15603)
                     /locus_tag="ACHBBW_001388"
                     /pseudo
     CDS             complement(14971..15603)
                     /locus_tag="ACHBBW_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15770..16531
                     /locus_tag="ACHBBW_001389"
     CDS             15770..16531
                     /locus_tag="ACHBBW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153075.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16557..19148
                     /gene="mprF"
                     /locus_tag="ACHBBW_001390"
     CDS             16557..19148
                     /gene="mprF"
                     /locus_tag="ACHBBW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9153076.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19336..20523)
                     /gene="tuf"
                     /locus_tag="ACHBBW_001391"
     CDS             complement(19336..20523)
                     /gene="tuf"
                     /locus_tag="ACHBBW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9153077.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20891..21247
                     /locus_tag="ACHBBW_001392"
     CDS             20891..21247
                     /locus_tag="ACHBBW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9153078.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21488..22492)
                     /locus_tag="ACHBBW_001393"
     CDS             complement(21488..22492)
                     /locus_tag="ACHBBW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9153079.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22658..23509
                     /locus_tag="ACHBBW_001394"
     CDS             22658..23509
                     /locus_tag="ACHBBW_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9153080.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23526..23771
                     /locus_tag="ACHBBW_001395"
     CDS             23526..23771
                     /locus_tag="ACHBBW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153081.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23958..24662
                     /locus_tag="ACHBBW_001396"
     CDS             23958..24662
                     /locus_tag="ACHBBW_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153082.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7011 a         5169 c         4355 g         8392 t
ORIGIN      
        1 acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag
       61 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
      121 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
      181 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
      241 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
      301 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
      361 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
      421 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
      481 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
      541 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
      601 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
      661 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
      721 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
      781 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
      841 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
      901 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
      961 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
     1021 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
     1081 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
     1141 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
     1201 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
     1261 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
     1321 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
     1381 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
     1441 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
     1501 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
     1561 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
     1621 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
     1681 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
     1741 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
     1801 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
     1861 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
     1921 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
     1981 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
     2041 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
     2101 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
     2161 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
     2221 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
     2281 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
     2341 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
     2401 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
     2461 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
     2521 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
     2581 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
     2641 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
     2701 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
     2761 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
     2821 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
     2881 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
     2941 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
     3001 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
     3061 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
     3121 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
     3181 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
     3241 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
     3301 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
     3361 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
     3421 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
     3481 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
     3541 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
     3601 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
     3661 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
     3721 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
     3781 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
     3841 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
     3901 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
     3961 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
     4021 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
     4081 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
     4141 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
     4201 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
     4261 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
     4321 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
     4381 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
     4441 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
     4501 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
     4561 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
     4621 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
     4681 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
     4741 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
     4801 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
     4861 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
     4921 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
     4981 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
     5041 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
     5101 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
     5161 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
     5221 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
     5281 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
     5341 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
     5401 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
     5461 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
     5521 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
     5581 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
     5641 ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg
     5701 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
     5761 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
     5821 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
     5881 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata
     5941 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
     6001 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
     6061 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
     6121 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
     6181 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
     6241 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
     6301 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
     6361 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
     6421 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
     6481 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
     6541 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
     6601 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
     6661 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
     6721 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
     6781 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
     6841 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
     6901 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
     6961 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
     7021 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
     7081 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
     7141 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
     7201 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
     7261 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
     7321 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
     7381 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
     7441 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
     7501 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
     7561 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
     7621 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
     7681 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
     7741 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
     7801 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
     7861 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
     7921 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
     7981 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
     8041 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
     8101 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
     8161 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
     8221 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
     8281 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc
     8341 ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt
     8401 ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag
     8461 cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact tcttccaatg
     8521 ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag
     8581 tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg
     8641 ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac
     8701 cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt
     8761 ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt
     8821 gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa
     8881 tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg
     8941 tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca
     9001 gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt
     9061 tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt
     9121 cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac
     9181 ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag
     9241 aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg
     9301 atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt
     9361 tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat
     9421 atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg
     9481 tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg
     9541 ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt
     9601 tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc
     9661 agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac
     9721 cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa
     9781 aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac
     9841 ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga
     9901 aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg
     9961 tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc
    10021 ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct
    10081 gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac
    10141 aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc
    10201 taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg
    10261 agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag
    10321 cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat
    10381 ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt
    10441 cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa
    10501 tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa
    10561 aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct
    10621 tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc
    10681 tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag
    10741 tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa
    10801 caatccagag cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag
    10861 aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa
    10921 cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg
    10981 atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg
    11041 attttggaca gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc
    11101 tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa
    11161 cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga
    11221 aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt
    11281 tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc
    11341 gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt
    11401 tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc
    11461 agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt
    11521 caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat
    11581 tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa
    11641 agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca
    11701 ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga
    11761 agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca
    11821 aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga
    11881 tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac
    11941 ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa
    12001 tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg
    12061 cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt
    12121 tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat
    12181 cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag
    12241 caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg
    12301 caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca
    12361 ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt
    12421 gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga
    12481 cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag
    12541 ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct
    12601 caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta
    12661 cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca
    12721 aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt
    12781 tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat
    12841 aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca
    12901 taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca
    12961 ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg
    13021 tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg
    13081 tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg
    13141 ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa
    13201 atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg
    13261 aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag
    13321 cataattatc tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc
    13381 ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa
    13441 gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc
    13501 ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt
    13561 taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat
    13621 ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat
    13681 acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa
    13741 aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt
    13801 cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc
    13861 cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc
    13921 tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa
    13981 tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta
    14041 tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta
    14101 ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg
    14161 gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg
    14221 ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc
    14281 ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc
    14341 gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata
    14401 cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg
    14461 tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg
    14521 atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca
    14581 gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa
    14641 aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga
    14701 aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc
    14761 tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct
    14821 gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact
    14881 tcttgatttt ccattttttg ccttttttcc actatttgcg tactccttct ttcatttaat
    14941 tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat
    15001 tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag
    15061 atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc
    15121 atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt
    15181 ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac
    15241 agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt
    15301 gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact
    15361 ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt
    15421 gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg
    15481 atagctttcc actcgtttta ccgaacgata agttcttcct cataaaaaca acaccgcacc
    15541 aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt
    15601 catttcatca cctagttatt ttgacatctt tttttatctt actatttctt tatcttcttg
    15661 gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt
    15721 tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa tgactgatat
    15781 aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat
    15841 gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc tctttttact
    15901 gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac tcgaacgcta
    15961 ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg aagcatctat
    16021 gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg aaaaaatcga
    16081 tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat ttgccagcag
    16141 cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg ttaaaggcgt
    16201 tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt tactctcttt
    16261 atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc atgacatcgg
    16321 gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa
    16381 agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa aagcttcctt
    16441 tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac gcttcaatcg
    16501 agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg atttatttgt
    16561 taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca atctttttga
    16621 tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact ctatctatcc
    16681 agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta atgctattga
    16741 ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat cggatgcttg
    16801 aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat acgatcaaca
    16861 atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta tatggacaaa
    16921 agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt ttgatggctg
    16981 gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca gttgctcctt
    17041 ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct gtcgtctttt
    17101 tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa actcaaggac
    17161 agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc ctttctgttg
    17221 gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc attgctgctt
    17281 cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat gtaatgatga
    17341 ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct
    17401 atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc ttcaaaaata
    17461 tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca acagaaattg
    17521 cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta ctttctgcca
    17581 ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca ttgaaatttc
    17641 attttattat ccaatttcct agtatactat tgggatttct tttaatcatt atgggcagag
    17701 ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt gttctagctt
    17761 tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg tctattctgc
    17821 ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc tattcatggg
    17881 agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac atcgttattg
    17941 gggtatacaa tctgccggat ttccctcatc ggcgtcacca ttttatttcc tttttcttat
    18001 ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt agtttcatga
    18061 tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa gcctttaatg
    18121 aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt caattgatct
    18181 tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact gttcttctcc
    18241 aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt aagaaagaag
    18301 attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc tatttaccag
    18361 ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc tacgatttca
    18421 ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca gggaaaaaaa
    18481 tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca tttgatgtct
    18541 tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct gataattggt
    18601 tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat tatctacaaa
    18661 gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt gcaacgatca
    18721 tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat gattctaaaa
    18781 aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat atgaaagaag
    18841 aacatatcca atggttcaat ttagggatgg cccctctttc aaatgtcgga acttccagaa
    18901 agagtttttt acaggaaagg attgcatact tggtttacga gtttggttct catttttatt
    18961 ctttccatgg attaagagaa tataaaagca agtacgctac aaactgggtc tcacgttata
    19021 ctctatattc tagagatagt tggattgcat atgttatgat tgctttgctg attattgata
    19081 atgcgccagt tgagcagaca caaggtagaa taagtggttt gaaaaaatgg atacgaagaa
    19141 agtattaaaa actgcatacc gatagatgaa cactctcatg atgaataaaa gagactgaag
    19201 taaaacgaaa gttcatgctt ctacaatctc ttcttataca attgaagaac aaagagcggt
    19261 ccaaacagaa atcctgtttg gaccgctctt ttattttaaa catttttcat taccaaaata
    19321 ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc
    19381 gccttcacga attgagaatg tcgttccgtt ttcaacagcg attggatgga tcaagtcaac
    19441 atcaatcgtc acgttgtcac caggcatgac catttctgta tcttctggca atgtgattgt
    19501 tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga tagttgttga agaatggagt
    19561 atgacgtcca ccttcttctt ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg
    19621 agtaatggaa cctggtttag caagtacttg tccacgttcg atatcgtcac gttggatccc
    19681 ccgtaacaac acgcctacgt tatccccagc ttccccgtaa tctaacgttt tacggaacat
    19741 ttctacacct gttacaactg ctttttgtgt ttcaggtttg atccctacga tctctacctc
    19801 atcaccgaca cgaacagcac cacgatcaat acgacctgag gcaacagttc ctcgacctgt
    19861 aattgagaag acgtcttcca ctggcaatag taatggttta tctgtatcac gttctggtgt
    19921 tgggatatat tcatctactg tatccatcaa ttccataata gcagcttcag catctggatc
    19981 gccttgcaat gcttttaatg cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc
    20041 atattcgctc aataactcgc gaacttccat ttctaccaaa tcgatcaatt cttcatcatc
    20101 gactaaatca actttgttca agaaaacaat caggtatttt acaccaactt ggcgtgacaa
    20161 taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc
    20221 accatccatt tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc
    20281 atcaatatgc gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg tattgatcgt
    20341 aatcccacgt tcacgttctt ctggagctgc atcaatacta gcatagtctt gaggatttgc
    20401 taggcctttt ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc
    20461 aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt ctgtcataat gttcttttgc
    20521 cataatttac aaacctccta aataatatag atcactgaaa aatttcagct ctcacctttt
    20581 tattatagtc tttttttgac taaatcaaaa agaatacgtc cgacttactt tttgtaagat
    20641 tcgaactaag cttattatac gtcaatattg gtcaaagtca actaagagca ctcgtctgtc
    20701 ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt gttttttgag aaaaacttca
    20761 agatagtcct taactttctt ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc
    20821 agctgttgcc aacaactttc aatagacgaa atcttctatt tcgttaataa taaggaaaga
    20881 ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt ttaggcatat ttctcggcgg
    20941 aagttttgca gcttatcatt atttttacgg cggcgaagct tattatacaa aaatcacgac
    21001 gaatggcgaa atgtcagcta cccaagcaga taacggagag aaattcacga cttataccta
    21061 caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag
    21121 agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt
    21181 attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga
    21241 taattagtaa atggtcttaa aattttatta gataaaaaga ggctgggaca gaagcgttta
    21301 actccaagaa ataagaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
    21361 agacaaaagt gttctttact tttgtctcac gctctttttt tgttatattc gtcttttccg
    21421 atccttctct tgacacaatt catttaactg atcactggta cttgaacacc taatattcta
    21481 gtcattttta taaacgattc aatacttcat ccacattttt gatcatcacg gatatcgtac
    21541 catccggatt attcacaaat tcaatcaaat ctgggttgcg atatacatct actggtacga
    21601 tcagttcgat cccgtttgat agcttgaatt tttgttttcc aaattttttc tcagaaattt
    21661 cttgtacttc ttttacagga ggtgcttctg ggacaaatcc ttgctcttgt acttcttctt
    21721 taaaagccat ttttgccgtg atgttttcct tgaagacttt ttccgcaatc atctcatgat
    21781 ccaaccgacc tttttcttct atcgtatcat agacagcttc tttcaaatca gccatgatat
    21841 caaattcttc tgtctcaaat ttttttccaa gatgcttggc tactttttgg atcactttta
    21901 cattttcttc taaagaaggt gcaggaacac tttcgatcac tcttccagaa aaataaagct
    21961 cttttttgcc agaaaactca tatttttttt caattaattc gtaggtccaa tcagtcaaat
    22021 tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc tagaattgct cgattcaaaa
    22081 tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa gtgagtgtaa gcctcacgat
    22141 aattgacttt caataaagat aagtacatca ctgtatctaa ttcatacaag acaacaaata
    22201 catctgcact aggtgcttct tcactttctt ggtagatatc gaaccaccgc tgaacgattg
    22261 cttcacttgc ttccacaaaa ttttcttgag cgattttaag tgattcaacg atcatgcttt
    22321 cttctgctag ttgtccagtt tttgtttgag cagaagagat tttttggatc ttcttttgaa
    22381 gaaaatcacg aatatattct tttgtcagat ccagctcttt ttgtgaaaag actggagaac
    22441 cagtttctct gtcaataata tgcagaattg ctttttttaa atagatatcc attttctcat
    22501 tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac cagaatcaaa atcattttct
    22561 tttaaacaaa aatttcaaga gtagaaacaa aatggattct ttttttcgaa aaatgcgcta
    22621 cacttatagt tgaacgaata aaaaaggagc tgaaagtatg aattcattaa caagcactta
    22681 tcgtctatca aatggttacg aaatcccggt agtaggattc ggcacatggc aaacacctga
    22741 tggggatgtc gctgtttcct ctgttaaaga agcgctggct gcaggttatc gccatatcga
    22801 tactgcccaa ggatacaaga atgaagaaag cgtcggccaa gcgattaagg aaagtggaat
    22861 tcctagagaa gagatattct tgacaacaaa attatggaat gcaaaccaca gctatgaact
    22921 tgtgatgtct tcttttgaag aatcattgaa gaagctccaa acagattatt tagatctgtt
    22981 tttgatccac tggccaaacc ctgttgcttt tcgtgataat tgggaacaag caaatgcaga
    23041 tacatggcga gcatttgaag aactgtatga agcaggtaaa atcaaagcaa tcggtgtgag
    23101 caatttctta ccccaccatc tagatacact agcaaaaaca gcaaaaatta tgccgatggt
    23161 caatcaagtt ttccttgcac caggcgaatt acagacaact gtagttgagt atgcaaaaaa
    23221 acatgatatg atcctcgaag catatagtcc attaggcact ggtaaaatct tcgatgtgcc
    23281 tgaaatgaaa caaatcgctg aagcacatga caagacgatt gcacaagttg ctttgcgttg
    23341 gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg acaccaagca gaatcaaaga
    23401 aaatactgaa ttatttgatt ttgaattaac agaagaagaa atgaaacaaa tcgatcagtt
    23461 agatggtgta gtcggcaaag caaaagaccc agatactacc caattttaag tcgaaaggtg
    23521 attttatgga tccaatcgaa cgcttgaatt ctctctcgga agaagttacc cagacttttc
    23581 attccgattt tgtctttttg atcgatgcag agaaaatcca acattttcct gcaagaaact
    23641 ggacacatga tcaaatcatc gaagaactga aaaaacggtt cgatcattcg ctaatggtca
    23701 aaccctggca cgaacatgaa gtcatttatt cacctgaact acctgttttt gccttgatac
    23761 cgaaaaaata gaaagaatgg aacggattat gtatatatac ataatccgtt ccatttttta
    23821 tttttctttc aaaaaacggg aaaatacgca aatttttctg ttgtaatttg cgtatacgca
    23881 agtttttttg cattactcct tttcatttgg ctgttttttt cttataatcg aaagggtatg
    23941 agcgaaggag agaacatatg aagaaaaaac agaaacaaaa attattaaat caattccgtc
    24001 cttcattgga tggtgtaaga ggacaattat tccgttctct agaagaagag agcttgcgac
    24061 gttatggttt gccgttacaa gtcatgcttg atcccaaaaa ccgtcaaatc ttaacgatcg
    24121 gattagctgg gaaaacaaat gaagatacac ggatcaacgt gccaatcgat gacaacttca
    24181 atactgtatt aaagagaatt cgttcaggag aaaacgggat tttcgaacga ttccgtgaca
    24241 atcttcttat tgaaatcgtg tcttactgga atgatcaacg attgaaaaac gatgaaccaa
    24301 ctgcgcaagc tgttgcgaca ccagttacag aagaaacagt cagtgtgcca gaagaaaaaa
    24361 cggaaaaacc aaaagcggac acaactgaaa atacacgctc aactgatacg aatgccgctt
    24421 taagttttgc tgatttttca aaagccgttg ctgagtatcc taaattttac acagaacaaa
    24481 atgaacatga agtagttatt aatgaaaaaa atgctgatga acctcgtaaa ttagcaacta
    24541 tttctatgac tactgaaaat gagttcgtta tcgaaaaagc actcgaacgg aaatacaaag
    24601 tcaaattgac tttgattcct ttgatcgaac aatttgcagc gacagcgatt actgatcgat
    24661 aaaaattcta tttgtcataa aaggctggga tttaaaatcc cagccttttt agtcatataa
    24721 taatttctac agcaagtaaa aagaaattcc ttctaacatc aggctcaaat atcagcttga
    24781 cctgtccaac aaatcaccta tgatcctaca atgttaggcg acttctcttc tacctgaaga
    24841 tatcttggag ttgcctgatt tcttaagatg cctaacctta actataaaaa attctaataa
    24901 taaacatatt ttctgataga gaaaaaa
//
LOCUS       DAYYKC010000036        24844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000036 DAYYKC010000000
VERSION     DAYYKC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.036"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1946
                     /locus_tag="ACHBBW_001397"
     CDS             48..1946
                     /locus_tag="ACHBBW_001397"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9153083.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2276..2995)
                     /locus_tag="ACHBBW_001398"
     CDS             complement(2276..2995)
                     /locus_tag="ACHBBW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9153084.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    3185..3232
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3332..3664
                     /locus_tag="ACHBBW_001399"
     CDS             3332..3664
                     /locus_tag="ACHBBW_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153085.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3651..5654
                     /locus_tag="ACHBBW_001400"
     CDS             3651..5654
                     /locus_tag="ACHBBW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9153086.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5673..6143
                     /locus_tag="ACHBBW_001401"
     CDS             5673..6143
                     /locus_tag="ACHBBW_001401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9153087.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6164..6748
                     /locus_tag="ACHBBW_001402"
     CDS             6164..6748
                     /locus_tag="ACHBBW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM9153088.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6763..7749
                     /locus_tag="ACHBBW_001403"
     CDS             6763..7749
                     /locus_tag="ACHBBW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9153089.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7739..8050
                     /locus_tag="ACHBBW_001404"
     CDS             7739..8050
                     /locus_tag="ACHBBW_001404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9153090.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8072..9853
                     /locus_tag="ACHBBW_001405"
     CDS             8072..9853
                     /locus_tag="ACHBBW_001405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9153091.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9846..11222
                     /locus_tag="ACHBBW_001406"
     CDS             9846..11222
                     /locus_tag="ACHBBW_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9153092.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11229..11864
                     /locus_tag="ACHBBW_001407"
     CDS             11229..11864
                     /locus_tag="ACHBBW_001407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9153093.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11894..13249
                     /locus_tag="ACHBBW_001408"
     CDS             11894..13249
                     /locus_tag="ACHBBW_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9153094.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13441..14835)
                     /locus_tag="ACHBBW_001409"
     CDS             complement(13441..14835)
                     /locus_tag="ACHBBW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9153095.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14927..15898
                     /locus_tag="ACHBBW_001410"
     CDS             14927..15898
                     /locus_tag="ACHBBW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9153096.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15895..16677
                     /locus_tag="ACHBBW_001411"
     CDS             15895..16677
                     /locus_tag="ACHBBW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9153097.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16846..17217)
                     /locus_tag="ACHBBW_001412"
     CDS             complement(16846..17217)
                     /locus_tag="ACHBBW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9153098.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17351..17794
                     /locus_tag="ACHBBW_001413"
     CDS             17351..17794
                     /locus_tag="ACHBBW_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9153099.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17837..18646)
                     /gene="yidA"
                     /locus_tag="ACHBBW_001414"
     CDS             complement(17837..18646)
                     /gene="yidA"
                     /locus_tag="ACHBBW_001414"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9153100.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18665..20032)
                     /locus_tag="ACHBBW_001415"
     CDS             complement(18665..20032)
                     /locus_tag="ACHBBW_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9153101.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20016..20846)
                     /locus_tag="ACHBBW_001416"
     CDS             complement(20016..20846)
                     /locus_tag="ACHBBW_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9153102.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21009..21446
                     /locus_tag="ACHBBW_001417"
     CDS             21009..21446
                     /locus_tag="ACHBBW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9153103.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21522..22142
                     /gene="rpoE"
                     /locus_tag="ACHBBW_001418"
     CDS             21522..22142
                     /gene="rpoE"
                     /locus_tag="ACHBBW_001418"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9153104.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22331..22837
                     /locus_tag="ACHBBW_001419"
     CDS             22331..22837
                     /locus_tag="ACHBBW_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9153105.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22854..23753
                     /locus_tag="ACHBBW_001420"
     CDS             22854..23753
                     /locus_tag="ACHBBW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9153106.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23840..24724
                     /locus_tag="ACHBBW_001421"
     CDS             23840..24724
                     /locus_tag="ACHBBW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9153107.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         8081 a         4327 c         5303 g         7133 t
ORIGIN      
        1 ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt
       61 tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc
      121 tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg
      181 aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca
      241 aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg
      301 cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga
      361 tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc
      421 gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc
      481 gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg
      541 aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc atcgatcatt
      601 tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg atggatcgaa
      661 tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg tggattggtg
      721 cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca cgctataatt
      781 atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt tcttttgctc
      841 atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa
      901 gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa ttcaaatcag
      961 aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa
     1021 aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag ttgaaaggaa
     1081 cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag gaacaaaaag
     1141 tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa catatgacgt
     1201 ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg tttcatggct
     1261 gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa tcttatcgcg
     1321 gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa gatctgactg
     1381 caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt
     1441 tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag cctcatacgc
     1501 acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc agtgaaaaaa
     1561 ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg cacacgccag
     1621 taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt gtcattgacg
     1681 gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca ctattatgca
     1741 attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa caacaatttg
     1801 caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac
     1861 tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag ctcaaaaggc
     1921 tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc catagactgg
     1981 agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt tcttctgcca
     2041 actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt tgaaattgct
     2101 ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa
     2161 gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa
     2221 tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa gtctattaaa
     2281 attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg tgggtaagtt
     2341 ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga accgcttcaa
     2401 cgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct ttgactgcct
     2461 gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac aaactgtctg
     2521 aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta
     2581 ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa acgacattca
     2641 atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat gcttccaaga
     2701 tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt ggatgaggat
     2761 cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt ttgtcgattt
     2821 gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga ggcaatcctt
     2881 gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga tttccttgga
     2941 aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt ttcatttgta
     3001 ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact atgtaagact
     3061 tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa
     3121 attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata
     3181 aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat
     3241 tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa
     3301 agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca
     3361 aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa
     3421 gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact atcgacaaca
     3481 actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag aaaaagaaaa
     3541 aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa
     3601 gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt atggccgtca
     3661 ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag
     3721 cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga acaaacaacc
     3781 agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca aaattaagcg
     3841 ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa caccacggcg
     3901 atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa gatttggaag
     3961 ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat ttaaaagaaa
     4021 agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa tggcttcttc
     4081 attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga
     4141 tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt aagcttgatg
     4201 acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt
     4261 taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc aataaagaaa
     4321 cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag caattatcca
     4381 ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt ggctatatcc
     4441 aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa aaaataaaag
     4501 aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa
     4561 aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt
     4621 tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc
     4681 cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag
     4741 tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag
     4801 atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa ctacttgttc
     4861 taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata ccatcgatca
     4921 tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa ctgtttggca
     4981 tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat
     5041 ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca aaggaaaata
     5101 tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa gggatcctaa
     5161 ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca tttgtttacc
     5221 ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg attatccaaa
     5281 cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga ctgaccggct
     5341 atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca ggcggaagta
     5401 ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga ttcagtgtag
     5461 ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg ctaagtgcct
     5521 atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat acaggaggcg
     5581 gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa
     5641 aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt agtcaaaatg
     5701 gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca gggatcggtt
     5761 cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca agtcaacccg
     5821 aaaaattcgg gcaggctctg attctgcaat tgcttccagg cacgcaaggt ttgtatggat
     5881 tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt gtacaaggac
     5941 tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca ggtattgccc
     6001 aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa
     6061 aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc gttatttcct
     6121 tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa
     6181 aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag aacaagaaga
     6241 gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa cagaacatca
     6301 aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg
     6361 ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt taagacgatt
     6421 gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat
     6481 caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg ctggagaaaa
     6541 atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac ttccctttat
     6601 gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg
     6661 agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt
     6721 tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta
     6781 aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg
     6841 attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca
     6901 tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt
     6961 ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg
     7021 cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa tcaaaatctg
     7081 gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat
     7141 acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga agtacgtgat
     7201 tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg
     7261 acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt
     7321 gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca
     7381 tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg
     7441 aactttgtag ataaagatat ggaagattac acgaattttc tattagagac agattatgct
     7501 gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct tgaacagttg
     7561 aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg
     7621 ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt
     7681 atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag acaagtctat
     7741 gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg
     7801 ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc
     7861 aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac
     7921 gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg
     7981 atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa
     8041 tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc
     8101 cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt
     8161 gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca
     8221 agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc
     8281 tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc
     8341 attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc
     8401 cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt
     8461 gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat
     8521 ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga
     8581 tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa
     8641 atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat
     8701 cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt
     8761 acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga
     8821 tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt
     8881 aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt
     8941 attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg
     9001 aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc
     9061 cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg
     9121 tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg
     9181 aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt
     9241 atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa
     9301 agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg
     9361 gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc atatccttca
     9421 agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct
     9481 gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt
     9541 agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga
     9601 tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga
     9661 agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt
     9721 atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact
     9781 agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat
     9841 gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg
     9901 aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga
     9961 ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag
    10021 gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac
    10081 taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata
    10141 acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag
    10201 tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga
    10261 atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag
    10321 aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg
    10381 tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc
    10441 gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa
    10501 tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa
    10561 agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag
    10621 agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca
    10681 cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta
    10741 ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga
    10801 ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc
    10861 agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg
    10921 gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag
    10981 gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca
    11041 agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca
    11101 cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa
    11161 gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt
    11221 gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa
    11281 aacagttgac aactgccaca agagggcata aattattaaa agataagcaa gatgaactga
    11341 tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt
    11401 ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca
    11461 tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata
    11521 tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac
    11581 ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta
    11641 ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg
    11701 cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc
    11761 aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag
    11821 taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta
    11881 aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca
    11941 gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc
    12001 attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc
    12061 tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg
    12121 tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat
    12181 tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga
    12241 agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt
    12301 ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt
    12361 tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc
    12421 agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat
    12481 ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact
    12541 ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg
    12601 taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct
    12661 agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag
    12721 aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac
    12781 gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac
    12841 tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga
    12901 agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt
    12961 tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa
    13021 aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct
    13081 tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat
    13141 gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag
    13201 aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct
    13261 gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga
    13321 tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg
    13381 tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg
    13441 ttataattca tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc
    13501 aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa
    13561 gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg
    13621 gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc
    13681 tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc
    13741 taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc
    13801 agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg
    13861 aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta
    13921 gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt
    13981 tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc
    14041 ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg
    14101 cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg
    14161 cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc
    14221 aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc
    14281 agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag attctaagga
    14341 cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc
    14401 tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat
    14461 aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat
    14521 atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa
    14581 tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt
    14641 gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg
    14701 agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg
    14761 ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata
    14821 agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga
    14881 ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa
    14941 ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg
    15001 tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc
    15061 tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata
    15121 ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca
    15181 tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc
    15241 aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc
    15301 aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt
    15361 gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga
    15421 aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa
    15481 tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag
    15541 taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg
    15601 cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta
    15661 tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga
    15721 aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa
    15781 aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga
    15841 agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac
    15901 ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg
    15961 gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac
    16021 aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt
    16081 acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta
    16141 tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta
    16201 tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa
    16261 gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta
    16321 gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat
    16381 tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg
    16441 caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta
    16501 tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa
    16561 ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat
    16621 cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt
    16681 ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg
    16741 ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt
    16801 ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac
    16861 ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa
    16921 tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta atttttctag
    16981 atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc
    17041 atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt
    17101 aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata
    17161 aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga
    17221 ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt
    17281 tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg
    17341 gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac
    17401 ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc
    17461 aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc
    17521 gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg
    17581 agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata atccaggata
    17641 tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat
    17701 ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc
    17761 agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc
    17821 tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt
    17881 cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg
    17941 ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca
    18001 tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac
    18061 ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat
    18121 cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca
    18181 tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg
    18241 gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt
    18301 agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc attacttcga
    18361 tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt
    18421 gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact
    18481 cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac
    18541 cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat
    18601 ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata
    18661 tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag
    18721 tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga
    18781 aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg
    18841 ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt
    18901 gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa
    18961 caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc
    19021 ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat
    19081 catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat
    19141 catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt
    19201 gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca
    19261 gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca
    19321 tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga
    19381 tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt
    19441 ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac
    19501 tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct
    19561 tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag
    19621 tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag
    19681 agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct
    19741 gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc
    19801 agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc
    19861 cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt
    19921 tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta tggactggat
    19981 cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc
    20041 tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg
    20101 atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca
    20161 aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct
    20221 tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct
    20281 tttaaagcag attgataaaa tcgcttcatg acttgcccac gtttttcctg attaccgttg
    20341 acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca
    20401 aattttttgc catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg
    20461 aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa
    20521 gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa
    20581 attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct
    20641 gcatcttcca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct
    20701 aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa
    20761 tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc
    20821 tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct
    20881 acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg
    20941 acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag
    21001 aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa
    21061 aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg
    21121 gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg
    21181 taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga
    21241 tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta
    21301 tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag
    21361 cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt
    21421 atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat
    21481 tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg
    21541 aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac
    21601 gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat
    21661 cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca
    21721 gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga
    21781 tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta
    21841 aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg
    21901 atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg
    21961 atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg
    22021 acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag
    22081 acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat
    22141 aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg
    22201 tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata
    22261 aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa
    22321 ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca
    22381 cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta
    22441 cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa
    22501 ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat
    22561 ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat
    22621 tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt
    22681 agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg
    22741 tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta
    22801 ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt
    22861 cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag
    22921 ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa
    22981 agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa
    23041 caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag
    23101 gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata
    23161 ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa
    23221 atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa
    23281 attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc
    23341 ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa
    23401 gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag
    23461 atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca
    23521 aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa
    23581 aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt
    23641 tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc
    23701 ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa
    23761 gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa
    23821 aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc
    23881 cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca
    23941 aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa
    24001 aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt
    24061 acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt
    24121 caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct
    24181 gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc
    24241 gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac
    24301 atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa
    24361 agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta
    24421 tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt
    24481 tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac
    24541 agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt
    24601 tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt
    24661 attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca
    24721 gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg aacacataac
    24781 taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa
    24841 tttt
//
LOCUS       DAYYKC010000037        24673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000037 DAYYKC010000000
VERSION     DAYYKC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.037"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..808
                     /gene="purC"
                     /locus_tag="ACHBBW_001422"
     CDS             80..808
                     /gene="purC"
                     /locus_tag="ACHBBW_001422"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9153108.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            808..1062
                     /gene="purS"
                     /locus_tag="ACHBBW_001423"
     CDS             808..1062
                     /gene="purS"
                     /locus_tag="ACHBBW_001423"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9153109.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1064..1735
                     /gene="purQ"
                     /locus_tag="ACHBBW_001424"
     CDS             1064..1735
                     /gene="purQ"
                     /locus_tag="ACHBBW_001424"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9153110.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1736..3958
                     /gene="purL"
                     /locus_tag="ACHBBW_001425"
     CDS             1736..3958
                     /gene="purL"
                     /locus_tag="ACHBBW_001425"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9153111.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3943..5382
                     /gene="purF"
                     /locus_tag="ACHBBW_001426"
     CDS             3943..5382
                     /gene="purF"
                     /locus_tag="ACHBBW_001426"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9153112.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5405..6457
                     /gene="purM"
                     /locus_tag="ACHBBW_001427"
     CDS             5405..6457
                     /gene="purM"
                     /locus_tag="ACHBBW_001427"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9153113.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6454..7032
                     /gene="purN"
                     /locus_tag="ACHBBW_001428"
     CDS             6454..7032
                     /gene="purN"
                     /locus_tag="ACHBBW_001428"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9153114.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7029..8564
                     /gene="purH"
                     /locus_tag="ACHBBW_001429"
     CDS             7029..8564
                     /gene="purH"
                     /locus_tag="ACHBBW_001429"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9153115.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8580..9854
                     /gene="purD"
                     /locus_tag="ACHBBW_001430"
     CDS             8580..9854
                     /gene="purD"
                     /locus_tag="ACHBBW_001430"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9153116.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9976..10890
                     /locus_tag="ACHBBW_001431"
     CDS             9976..10890
                     /locus_tag="ACHBBW_001431"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9153117.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11023..12588)
                     /locus_tag="ACHBBW_001432"
     CDS             complement(11023..12588)
                     /locus_tag="ACHBBW_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153118.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12581..14032)
                     /locus_tag="ACHBBW_001433"
     CDS             complement(12581..14032)
                     /locus_tag="ACHBBW_001433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9153119.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14046..15062)
                     /locus_tag="ACHBBW_001434"
     CDS             complement(14046..15062)
                     /locus_tag="ACHBBW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153120.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15282..16370
                     /gene="chvE"
                     /locus_tag="ACHBBW_001435"
     CDS             15282..16370
                     /gene="chvE"
                     /locus_tag="ACHBBW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9153121.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16424..17950
                     /gene="mmsA"
                     /locus_tag="ACHBBW_001436"
     CDS             16424..17950
                     /gene="mmsA"
                     /locus_tag="ACHBBW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9153122.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17952..19157
                     /gene="mmsB"
                     /locus_tag="ACHBBW_001437"
     CDS             17952..19157
                     /gene="mmsB"
                     /locus_tag="ACHBBW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9153123.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19414..20391
                     /gene="guaC"
                     /locus_tag="ACHBBW_001438"
     CDS             19414..20391
                     /gene="guaC"
                     /locus_tag="ACHBBW_001438"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9153124.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20655..22079
                     /locus_tag="ACHBBW_001439"
     CDS             20655..22079
                     /locus_tag="ACHBBW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9153125.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22121..22378)
                     /locus_tag="ACHBBW_001440"
     CDS             complement(22121..22378)
                     /locus_tag="ACHBBW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153126.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22540..23649
                     /gene="pdhA"
                     /locus_tag="ACHBBW_001441"
     CDS             22540..23649
                     /gene="pdhA"
                     /locus_tag="ACHBBW_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9153127.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23652..24629
                     /locus_tag="ACHBBW_001442"
     CDS             23652..24629
                     /locus_tag="ACHBBW_001442"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9153128.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7986 a         4203 c         5578 g         6906 t
ORIGIN      
        1 gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa
       61 ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa
      121 aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc
      181 gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac
      241 aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt
      301 gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg
      361 aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat
      421 caaaccaatc attgaattct actataagga tgaccagttg gatgatccat ttatcaatga
      481 agagcatatc tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca
      541 agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt
      601 agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac
      661 accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta
      721 ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga
      781 actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa
      841 gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat
      901 gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa
      961 gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg
     1021 gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat
     1081 cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg
     1141 cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt
     1201 actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc
     1261 tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg
     1321 caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga
     1381 gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac
     1441 ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta
     1501 tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta
     1561 taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa
     1621 tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga
     1681 tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa
     1741 aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg
     1801 attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta
     1861 tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa
     1921 accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg
     1981 cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa
     2041 tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag
     2101 agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga
     2161 attagataat ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata
     2221 cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga
     2281 aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa
     2341 agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga
     2401 cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg
     2461 ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga
     2521 attaatcttg catcattcgg attctctaat aggtatacag gatatgggag cagcaggact
     2581 tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga
     2641 tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca
     2701 agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca
     2761 gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct
     2821 atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc
     2881 agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc
     2941 ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac
     3001 gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt
     3061 tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc
     3121 tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat
     3181 agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac
     3241 cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc
     3301 agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt
     3361 ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt
     3421 cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct
     3481 aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca
     3541 gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca
     3601 agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga
     3661 aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt
     3721 acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac
     3781 agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc
     3841 aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat
     3901 agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag
     3961 agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg
     4021 gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc
     4081 aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa
     4141 gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca
     4201 acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag
     4261 tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta
     4321 gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc
     4381 cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa
     4441 ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat
     4501 ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa
     4561 acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata
     4621 gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct
     4681 atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat
     4741 gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca
     4801 gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct
     4861 agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc
     4921 cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga
     4981 gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc
     5041 agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct
     5101 tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc
     5161 attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt
     5221 tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca
     5281 ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa
     5341 aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga
     5401 gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata
     5461 tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg
     5521 cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt
     5581 ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa
     5641 acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg
     5701 cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt
     5761 ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg
     5821 cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt
     5881 tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga
     5941 tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa
     6001 aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac
     6061 acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat
     6121 caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat
     6181 cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct
     6241 tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat
     6301 cttcaatatg ggcatcggta tggtcctggc agtccaagaa tcgaaagtaa aagaaatagt
     6361 acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga
     6421 aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga
     6481 aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg
     6541 attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg
     6601 agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa
     6661 gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg
     6721 cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg
     6781 tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta
     6841 aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc
     6901 cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc
     6961 catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc
     7021 tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg
     7081 cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt
     7141 tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga
     7201 tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg
     7261 atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt
     7321 gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag
     7381 ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg
     7441 aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc
     7501 agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata
     7561 ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac
     7621 cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc
     7681 atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa
     7741 aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac
     7801 ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg
     7861 gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg
     7921 tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac
     7981 agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg
     8041 aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa
     8101 atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc
     8161 tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag
     8221 aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg
     8281 tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt
     8341 cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg
     8401 atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa
     8461 tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg
     8521 atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa
     8581 tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag
     8641 ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg
     8701 gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga
     8761 aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg
     8821 atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg
     8881 aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat
     8941 ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa
     9001 tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga
     9061 aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca
     9121 aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc
     9181 gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg
     9241 ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa
     9301 agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac
     9361 taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa
     9421 aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt
     9481 tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa
     9541 agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag
     9601 aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg
     9661 ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag
     9721 aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata
     9781 aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac
     9841 tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag
     9901 cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa
     9961 taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca
    10021 gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca
    10081 gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt
    10141 gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt
    10201 caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt
    10261 gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt
    10321 catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa
    10381 cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg
    10441 ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat
    10501 actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat
    10561 gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca
    10621 taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac
    10681 caaacattag aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct
    10741 tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga
    10801 atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg
    10861 taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat
    10921 aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt
    10981 ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt
    11041 aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata
    11101 actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc
    11161 tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa
    11221 tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg
    11281 ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa
    11341 tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa
    11401 gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa
    11461 gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca
    11521 gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt
    11581 gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga
    11641 caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat
    11701 caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg
    11761 cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac
    11821 agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata
    11881 actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga
    11941 ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact
    12001 gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat
    12061 ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt
    12121 aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc
    12181 agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc
    12241 ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat
    12301 tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg
    12361 aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc
    12421 agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc
    12481 agcttcgccg acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa
    12541 gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc
    12601 caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc
    12661 tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt
    12721 tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta
    12781 tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca
    12841 tctcatcaat tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc
    12901 catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa
    12961 aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga
    13021 tgactagata atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt
    13081 tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa
    13141 agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag
    13201 taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt
    13261 tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt
    13321 gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt
    13381 ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat
    13441 tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa
    13501 tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa
    13561 aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt
    13621 cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt
    13681 ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg
    13741 tattattttt gatctctcga atatcattcc taagttcttc taggatattt tcctcattga
    13801 actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat
    13861 caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca
    13921 aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca
    13981 tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc
    14041 tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg
    14101 gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta
    14161 acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt
    14221 cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct
    14281 tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca
    14341 ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc
    14401 tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga
    14461 ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa
    14521 aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt
    14581 ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt
    14641 ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga
    14701 atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag
    14761 tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac
    14821 gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg
    14881 taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc
    14941 tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt
    15001 gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc
    15061 actttaaatc actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca
    15121 aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa
    15181 agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta
    15241 ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa
    15301 agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt
    15361 caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag
    15421 aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa
    15481 cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga
    15541 ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg
    15601 tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga
    15661 attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag
    15721 cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat
    15781 tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg
    15841 ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca
    15901 gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt
    15961 taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa
    16021 gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac
    16081 ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac
    16141 aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca
    16201 aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg
    16261 tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag
    16321 cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa
    16381 acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt
    16441 aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct
    16501 gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac
    16561 attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta
    16621 tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat
    16681 cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg
    16741 aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga
    16801 actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt
    16861 agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat
    16921 tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat
    16981 ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat
    17041 cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga
    17101 aaatgaaatg atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga
    17161 tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac
    17221 ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa
    17281 aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat
    17341 gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc
    17401 agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga
    17461 ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag
    17521 cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc
    17581 agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag
    17641 tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga
    17701 tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta
    17761 cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact
    17821 tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg
    17881 agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga
    17941 ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt
    18001 tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac
    18061 tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta
    18121 accttgttct gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc
    18181 tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg
    18241 gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag
    18301 taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt
    18361 ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag
    18421 gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag
    18481 acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga
    18541 tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag
    18601 tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa
    18661 taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga
    18721 tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga
    18781 cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct
    18841 gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat
    18901 tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat
    18961 attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg
    19021 gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc
    19081 agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga
    19141 aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt
    19201 gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa
    19261 taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta
    19321 attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt
    19381 tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt
    19441 caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca
    19501 ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat
    19561 gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat
    19621 gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc
    19681 agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta
    19741 aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg
    19801 attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca
    19861 ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt
    19921 ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg
    19981 gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc
    20041 agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt
    20101 tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg
    20161 tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa
    20221 ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa
    20281 caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa
    20341 gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat
    20401 aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa
    20461 aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa
    20521 catcattatt cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc
    20581 aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata
    20641 gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa
    20701 ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg
    20761 aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat
    20821 tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta
    20881 atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc
    20941 ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa
    21001 aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg
    21061 agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag
    21121 gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat
    21181 tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac
    21241 aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat
    21301 tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga
    21361 tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca
    21421 acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag
    21481 tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt
    21541 catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat
    21601 acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac
    21661 ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata
    21721 tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag
    21781 atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat
    21841 cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact tgggatggga
    21901 aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa
    21961 gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa
    22021 accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat
    22081 ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct
    22141 tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc
    22201 cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt
    22261 taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat
    22321 aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa
    22381 tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat
    22441 ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg
    22501 aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg
    22561 atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt
    22621 gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc
    22681 gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg
    22741 aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg
    22801 gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct
    22861 caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct
    22921 gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca
    22981 caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc
    23041 gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat
    23101 ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc
    23161 tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct
    23221 gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa
    23281 atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat
    23341 cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca
    23401 gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa
    23461 ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa
    23521 gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac
    23581 atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag
    23641 tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg
    23701 aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag
    23761 ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc
    23821 ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc
    23881 cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc
    23941 aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt
    24001 cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga
    24061 tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac
    24121 tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat
    24181 atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag
    24241 cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag
    24301 caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc
    24361 gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg
    24421 tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga
    24481 tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata
    24541 cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag
    24601 acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt
    24661 atcaatttaa att
//
LOCUS       DAYYKC010000038        24435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000038 DAYYKC010000000
VERSION     DAYYKC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.038"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..536)
                     /locus_tag="ACHBBW_001443"
     CDS             complement(<1..536)
                     /locus_tag="ACHBBW_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9153129.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGA"
     gene            complement(548..1240)
                     /locus_tag="ACHBBW_001444"
     CDS             complement(548..1240)
                     /locus_tag="ACHBBW_001444"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9153130.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1259..1543)
                     /gene="macP"
                     /locus_tag="ACHBBW_001445"
     CDS             complement(1259..1543)
                     /gene="macP"
                     /locus_tag="ACHBBW_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9153131.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1556..2119)
                     /locus_tag="ACHBBW_001446"
     CDS             complement(1556..2119)
                     /locus_tag="ACHBBW_001446"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9153132.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2347..3009
                     /locus_tag="ACHBBW_001447"
     CDS             2347..3009
                     /locus_tag="ACHBBW_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9153133.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3133..4329
                     /locus_tag="ACHBBW_001448"
     CDS             3133..4329
                     /locus_tag="ACHBBW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9153134.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4430..5806)
                     /locus_tag="ACHBBW_001449"
     CDS             complement(4430..5806)
                     /locus_tag="ACHBBW_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153135.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5843..6710)
                     /locus_tag="ACHBBW_001450"
                     /pseudo
     CDS             complement(5843..6710)
                     /locus_tag="ACHBBW_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6799..7824)
                     /locus_tag="ACHBBW_001451"
     CDS             complement(6799..7824)
                     /locus_tag="ACHBBW_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9153136.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7842..8516)
                     /locus_tag="ACHBBW_001452"
     CDS             complement(7842..8516)
                     /locus_tag="ACHBBW_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9153137.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8700..9593)
                     /locus_tag="ACHBBW_001453"
     CDS             complement(8700..9593)
                     /locus_tag="ACHBBW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9153138.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9667..11364)
                     /locus_tag="ACHBBW_001454"
     CDS             complement(9667..11364)
                     /locus_tag="ACHBBW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9153139.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11357..12670)
                     /locus_tag="ACHBBW_001455"
     CDS             complement(11357..12670)
                     /locus_tag="ACHBBW_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9153140.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12670..16611)
                     /locus_tag="ACHBBW_001456"
     CDS             complement(12670..16611)
                     /locus_tag="ACHBBW_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9153141.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16720..17178)
                     /locus_tag="ACHBBW_001457"
     CDS             complement(16720..17178)
                     /locus_tag="ACHBBW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9153142.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17476..17994)
                     /locus_tag="ACHBBW_001458"
     CDS             complement(17476..17994)
                     /locus_tag="ACHBBW_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9153143.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17996..18613)
                     /locus_tag="ACHBBW_001459"
     CDS             complement(17996..18613)
                     /locus_tag="ACHBBW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9153144.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18610..19464)
                     /locus_tag="ACHBBW_001460"
     CDS             complement(18610..19464)
                     /locus_tag="ACHBBW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153145.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19461..20372)
                     /locus_tag="ACHBBW_001461"
     CDS             complement(19461..20372)
                     /locus_tag="ACHBBW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153146.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20413..21747)
                     /locus_tag="ACHBBW_001462"
     CDS             complement(20413..21747)
                     /locus_tag="ACHBBW_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153147.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21955..22785
                     /locus_tag="ACHBBW_001463"
     CDS             21955..22785
                     /locus_tag="ACHBBW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM9153148.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23029..23278)
                     /locus_tag="ACHBBW_001464"
                     /pseudo
     CDS             complement(23029..23278)
                     /locus_tag="ACHBBW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23469..23663)
                     /locus_tag="ACHBBW_001465"
     CDS             complement(23469..23663)
                     /locus_tag="ACHBBW_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153149.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24168..24353)
                     /locus_tag="ACHBBW_001466"
     CDS             complement(24168..24353)
                     /locus_tag="ACHBBW_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153150.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         7214 a         5306 c         4224 g         7691 t
ORIGIN      
        1 tctgcgccta gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa
       61 cttgccacgg aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct
      121 gtatgcaaca cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt
      181 tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc
      241 caataaacat tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat
      301 tcccgtccac ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag
      361 gaatcttttg gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac
      421 ttctttgtat gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc
      481 tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta
      541 gtcctcctta tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg
      601 cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg
      661 cacgtacgat caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt
      721 ccatttcgca agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg
      781 agtcgacaaa tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt
      841 ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc
      901 ctggtaattg tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt
      961 tgtctgatat aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg
     1021 tattgatcac catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta
     1081 agacttttcc tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa
     1141 taaataaagc gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt
     1201 ttacttcttc ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct
     1261 atataaatgc gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt
     1321 tcatcaaaaa agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga
     1381 ttggtttatt ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct
     1441 gataagctgt ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc
     1501 gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact
     1561 ttcctttggt caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca
     1621 actgatcttt catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt
     1681 cgtcttcatc ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat
     1741 gaaggacttc atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact
     1801 tcgcacgata gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc
     1861 cttctcctgg atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc
     1921 gaaattgttt gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc
     1981 caggatgaaa taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta
     2041 cagcaacatc aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa
     2101 attgctcatt gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt
     2161 catttttcaa tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt
     2221 ctctaaatat agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa
     2281 ataagtatgt aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg
     2341 tttattatga taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt
     2401 gtccgaaaca atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt
     2461 ttgtggggac tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg
     2521 actgacgaac ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt
     2581 tttcgacaaa caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag
     2641 cagacagcca aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct
     2701 ttattcaaag aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat
     2761 acgcatctgc cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag
     2821 atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg
     2881 tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac
     2941 gtagagatat ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa
     3001 aaggattaac tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt
     3061 ttcgagtact ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag
     3121 gaggaatttt ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat
     3181 acacttgaag atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca
     3241 caacaagcgg agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa
     3301 ctaccggcgg aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat
     3361 gcacagtctg tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa
     3421 tcgatgttga atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa
     3481 ctgatgtatc gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc
     3541 ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa
     3601 aagattggtg cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg
     3661 ctaaaagata atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca
     3721 ctgaatattt atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt
     3781 ccagaagcga tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat
     3841 gattacacgc aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga
     3901 cagatcacga tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg
     3961 gctgaaaaga tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg
     4021 atcgcattga ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa
     4081 acaacaaacg acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca
     4141 gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat
     4201 ttgatcgaaa caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt
     4261 catgcagaag tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta
     4321 acccaataga tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa
     4381 ctgttgatgg gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat
     4441 tcatcttgct gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga
     4501 taccgaacac gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaaa
     4561 tggagacgat tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc
     4621 caaacatttt tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca
     4681 tgatattcgt tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg
     4741 ccacaccagc ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca
     4801 taaacttagg aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca
     4861 tcatcgagat cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca
     4921 ctgttccatg tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag
     4981 tatacggaac gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca
     5041 gtatcgaacc aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg
     5101 ccattgcaac ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca
     5161 caccggaaaa accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc
     5221 ctacaatgat aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac
     5281 gtaatgcttt cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg
     5341 caatcaagtc aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa
     5401 gttcggcccc taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag
     5461 aggaaaccgc tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt
     5521 caccaaatac aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa
     5581 attctgcgaa acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg
     5641 acagtgctgg accagcattt tgattggccg caacccctgt caccacaaat attcccgtac
     5701 cgatgatcgc accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct
     5761 cactcgtatg attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc
     5821 attttaggtt ggaaaacagc gattaaactg ttttcatata ccctaaaagg gataccacct
     5881 aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg
     5941 ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag
     6001 ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat
     6061 aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca
     6121 aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc
     6181 aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat
     6241 gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca
     6301 tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat
     6361 acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa
     6421 attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca
     6481 tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa
     6541 atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca
     6601 ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa
     6661 ccaatactac cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc
     6721 cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt
     6781 ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa
     6841 agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc
     6901 ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc
     6961 atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat
     7021 ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat
     7081 gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga agataaaagc
     7141 gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt
     7201 tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc
     7261 atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg
     7321 ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc
     7381 aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc
     7441 atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat
     7501 tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc
     7561 tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt
     7621 tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc
     7681 ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca
     7741 catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc
     7801 tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc
     7861 tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac
     7921 cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct
     7981 cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga
     8041 cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca
     8101 acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa
     8161 gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc
     8221 acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg
     8281 gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca
     8341 tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct
     8401 gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc
     8461 tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa
     8521 aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg
     8581 gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa
     8641 tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat
     8701 tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg
     8761 aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat
     8821 ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc
     8881 actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc
     8941 tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg
     9001 acttcataag cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt
     9061 tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa
     9121 tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct
     9181 aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag
     9241 atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc
     9301 gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc
     9361 gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt
     9421 tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct
     9481 gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat
     9541 tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc
     9601 accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt
     9661 tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat
     9721 caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga
     9781 cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat
     9841 ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg
     9901 aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc
     9961 ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc
    10021 ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa
    10081 tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc
    10141 actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg
    10201 ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt
    10261 gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa
    10321 atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc
    10381 ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc
    10441 ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc
    10501 aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac
    10561 agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc
    10621 tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct
    10681 aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata
    10741 caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac
    10801 catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg
    10861 agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc
    10921 cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat
    10981 acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat
    11041 cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa
    11101 atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc
    11161 accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc
    11221 ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac
    11281 tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc
    11341 cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag
    11401 cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg
    11461 ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt
    11521 tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag
    11581 agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt
    11641 gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg
    11701 cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag
    11761 gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat
    11821 ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag
    11881 caggagccat atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag
    11941 caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt
    12001 atggatctct tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg
    12061 ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga
    12121 tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat
    12181 cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa
    12241 ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta
    12301 gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca
    12361 tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg
    12421 ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga
    12481 tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca
    12541 tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat
    12601 gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca
    12661 gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc
    12721 tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc
    12781 tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt
    12841 tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt
    12901 tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc
    12961 attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt
    13021 tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga
    13081 agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata
    13141 ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt
    13201 catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac
    13261 ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat
    13321 gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc
    13381 agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag
    13441 atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata
    13501 gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta
    13561 tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc
    13621 taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt
    13681 gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg
    13741 ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg
    13801 caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat
    13861 gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg
    13921 ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc
    13981 tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc
    14041 tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc
    14101 tgcgccattg attggcatat tactgccttc aattagttta ttgatattca tgtggattgg
    14161 cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat
    14221 gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc
    14281 tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg
    14341 acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc
    14401 tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc
    14461 tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt
    14521 agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc
    14581 aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg
    14641 gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc
    14701 attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc
    14761 gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc
    14821 taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt
    14881 cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg
    14941 gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc
    15001 agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt
    15061 tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc
    15121 attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt
    15181 tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat
    15241 ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac
    15301 tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga
    15361 agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg
    15421 atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg
    15481 agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc
    15541 attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct
    15601 cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac
    15661 gattaggatt ttttctggat ttctattttc tccgccattg tctttataca atgtttcata
    15721 gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt
    15781 attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat
    15841 ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat
    15901 atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc
    15961 agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc
    16021 tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag
    16081 atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg
    16141 cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc
    16201 aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc
    16261 gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt
    16321 tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg
    16381 atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa
    16441 cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg
    16501 gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat
    16561 gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc
    16621 ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg
    16681 gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg
    16741 gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc
    16801 gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata
    16861 ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc
    16921 gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca
    16981 ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa
    17041 gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg
    17101 atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt
    17161 caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac
    17221 aatcaacaaa acaatgccca tctaattata atttaattta attttaattt taaatcaaat
    17281 gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat
    17341 aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa
    17401 ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt
    17461 attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat
    17521 agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat
    17581 caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc
    17641 tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact
    17701 tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt
    17761 gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata
    17821 ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc
    17881 attagagata ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg
    17941 cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt
    18001 cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa
    18061 acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa
    18121 gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga
    18181 aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa
    18241 agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc
    18301 ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg
    18361 ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg
    18421 aaccgagctt tttctcttca ttttttttgt aatgataaat catatacaga actgttcgac
    18481 ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga
    18541 taagacgtgg aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg
    18601 atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg
    18661 tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata
    18721 attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt
    18781 ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga
    18841 attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat
    18901 atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa
    18961 gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag
    19021 gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt
    19081 tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg
    19141 aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac
    19201 tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat
    19261 cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac
    19321 gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat
    19381 gattcctatt actgtcatga ggacataact tagtattttt tttgtttgcc gattcacact
    19441 ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac ccacttaggt
    19501 gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat cattgacata
    19561 gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg cagattataa
    19621 aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata agcttcttga
    19681 aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat aggagtgatt
    19741 aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat ttctgctgat
    19801 tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat tgctcctcca
    19861 gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc aaacagccaa
    19921 gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc agatggagaa
    19981 agcagctgca tccataagat gtagactgct actcctgata agactgccgg taaatagaaa
    20041 gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc taaaaacaca
    20101 ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt gtataacgat
    20161 ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc aatgaaattc
    20221 atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag cataggccct
    20281 agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc ccatcttgct
    20341 tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg tacctagaca
    20401 gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt gtgcatctgc
    20461 caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt cattcactaa
    20521 tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt taaagttttc
    20581 gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat tcactaaatt
    20641 ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt tttcttgtac
    20701 ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt ttgctccgta
    20761 tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt cttctttttc
    20821 tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca aactagagta
    20881 ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat cagatacacc
    20941 agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat cttgccagtc
    21001 ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt tttcatcttt
    21061 gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc ctaaattcca
    21121 taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta gtttacgtgc
    21181 tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc cagcttcttt
    21241 aaataacttt ttattataaa aaatgacttg tgtatctgta ttgaatggga tcgcataagg
    21301 atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag aagtgtcttt
    21361 ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg caacggaatt
    21421 tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata aagatttagt
    21481 ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt gtgattgatt
    21541 gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc aaaaggacca
    21601 gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg cacttgaact
    21661 ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg aacttgccag
    21721 caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt aaccgatgcc
    21781 ggttttaata taacgatatc attttgttct gaaaataaca atttataagg tatgattcta
    21841 aaaaaattat taatgatacc aatactttat ataattcgcg agcgctttct ttgaattttt
    21901 taatctttcg tagcagaatg agaatgtatt aaaaatagat taggaggaat tttgatgttt
    21961 agacaccaga aagaattgca gtttgaagca aaaccagatc gtccgaatcc attaatcgcg
    22021 aaatatctgc aggagctgat cggcggccaa tatggcgaga tgagtgtagc tatgcagtac
    22081 ctgttccaag gctggagctg ccgaggagaa gaaaaatata aagatatgct gatggacatt
    22141 gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg tttattagag
    22201 ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag atccaacaat cggtgccatt
    22261 atctctggaa tgaatccaca gcacgcgatc gtagatggct taggacctcg tcctgctgac
    22321 agcgtgggca atccatggag cggtgcctat atcattgcga gtggcaactt gttagcagac
    22381 tttagagcta atttaaatgc cgaatcacaa ggaagacttc aagtagcacg tatttacgaa
    22441 atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag agatagctat
    22501 catcaaaatc tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta
    22561 gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga cttctataac
    22621 ttctcagagg gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc actggatggc
    22681 gaaggcgaat atcaatggtt gaaagaacct aagatcgaag gcgctgcacc aaaattagaa
    22741 ccagtaacac caaaaatgta tgggacacct ccattcaaga aataaatatc tacccgtaga
    22801 aacacccgtt ttttgttcca cacgctttgt gagcaaaact gactaaagtc attaatcata
    22861 accacccttc aaaagggtgg ttttctctag agctataagc tcaaatcacc ggccagcgcc
    22921 taaagacgct ggctttcata ttttatgttc aagccatatt gcctttgcca gttttgccca
    22981 cctcttaaaa ggtttatgta ctactcctta gcccttaaaa ggatcgtctt actctcgtac
    23041 actcagctta tctaacgcac tatcatgttt ttcttgttct tgaatgtact ttttgatggt
    23101 cgcttcattt tgtcctactt ttgtcacata gtatccttcc acccaaaagt gtcgataccc
    23161 aaatttatat tttagatttg cacaatctct tgatgatctc ctgaatattg gctcgatatt
    23221 gattataaat tatttttctt ctatactttg gtgtgaatac aatgtggtat ttacacatct
    23281 actttgtacg ggctaaacta tttcccttta ttgctccacc cgcatagcaa gtggttttgt
    23341 atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa aacccgctgc
    23401 ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc
    23461 aatctatttt atttttttga ttttttataa atggaaaatg cttcaagaag tccaagaaat
    23521 aaagccgtat aaaaacaaga acctatgaaa tctttattta ttagatattc aacagaaatc
    23581 ccgataacac tagccaacaa tatatttatc agaagtgtaa aataaggatg ctttttatag
    23641 aatagaacta gtgttgtttt catagactct cctttcttca tatttccaat taatcacaaa
    23701 ttcacggcat tactagaaaa attaaagggg cgcagaagtg gaaaaatact cttcgacttt
    23761 ccctacaaac caggccatct tgtgtttaat aactatacca aatctaaggt acctctaaat
    23821 ctaaattcca tttgtggcgg tgacctccaa tttttaactc ttctatctcc catccagtat
    23881 acataacctt gatacttcga ccacttatat atcgaacaca caaataccta tccattgcag
    23941 gagtactacc taacttagtt gctacttgca tctaaataac agctgaaaag ataggactta
    24001 ctcttagtaa agtatctgaa ctattaattt ttaaactatc atttttatcc tcaactacat
    24061 gcacatctgt tgcaacttca ctttctgtct catccagata aatgattaag gaatcgggaa
    24121 ttgcaatatc ttcagctgac gctggaatag ttgagctaaa ttagtcatca tatctttatt
    24181 ataaaataac caacagcaaa aaatacaatg aaaaacaaac ttggaactaa tgtatcatta
    24241 ataaatgttg aaaaattcag caactcatta ctgtatgatt ttagcagccc aaccaacata
    24301 taccctataa aagctaataa gatggttagg aatcctttag aatttttact cattcataga
    24361 cacttcctta aataactttt attatagaag gtattgtaaa taataatatt gacaaaaagt
    24421 gactcaaaat aaaaa
//
LOCUS       DAYYKC010000039        24426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000039 DAYYKC010000000
VERSION     DAYYKC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.039"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHBBW_001467"
     CDS             complement(53..787)
                     /locus_tag="ACHBBW_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9153151.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHBBW_001468"
     CDS             1114..1524
                     /locus_tag="ACHBBW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9153152.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHBBW_001469"
     CDS             1517..2218
                     /locus_tag="ACHBBW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9153153.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHBBW_001470"
     CDS             2218..3804
                     /locus_tag="ACHBBW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153154.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHBBW_001471"
     CDS             3823..4044
                     /locus_tag="ACHBBW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9153155.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHBBW_001472"
     CDS             4041..4802
                     /locus_tag="ACHBBW_001472"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9153156.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHBBW_001473"
     CDS             5182..5691
                     /locus_tag="ACHBBW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9153157.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHBBW_001474"
     CDS             complement(5766..6305)
                     /locus_tag="ACHBBW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9153158.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBBW_001475"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBBW_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9153159.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHBBW_001476"
     CDS             7318..8169
                     /locus_tag="ACHBBW_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9153160.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHBBW_001477"
     CDS             8197..8832
                     /locus_tag="ACHBBW_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9153161.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHBBW_001478"
     CDS             8852..9625
                     /locus_tag="ACHBBW_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9153162.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHBBW_001479"
     CDS             10033..10830
                     /locus_tag="ACHBBW_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9153163.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHBBW_001480"
     CDS             10808..11221
                     /locus_tag="ACHBBW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9153164.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBBW_001481"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBBW_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9153165.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBBW_001482"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBBW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9153166.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHBBW_001483"
     CDS             15998..16558
                     /locus_tag="ACHBBW_001483"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9153167.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBBW_001484"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBBW_001484"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9153168.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHBBW_001485"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHBBW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9153169.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHBBW_001486"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHBBW_001486"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9153170.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHBBW_001487"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHBBW_001487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9153171.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHBBW_001488"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHBBW_001488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9153172.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHBBW_001489"
     CDS             21905..22483
                     /locus_tag="ACHBBW_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153173.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHBBW_001490"
     CDS             22535..23017
                     /locus_tag="ACHBBW_001490"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9153174.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHBBW_001491"
     CDS             23134..23508
                     /locus_tag="ACHBBW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153175.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24426
                     /locus_tag="ACHBBW_001492"
     CDS             23938..>24426
                     /locus_tag="ACHBBW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9153176.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4390 c         5213 g         6816 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggac
//
LOCUS       DAYYKC010000040        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000040 DAYYKC010000000
VERSION     DAYYKC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.040"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHBBW_001493"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHBBW_001493"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9153177.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHBBW_001494"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHBBW_001494"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9153178.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHBBW_001495"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHBBW_001495"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9153179.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHBBW_001496"
     CDS             complement(3373..4680)
                     /locus_tag="ACHBBW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9153180.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHBBW_001497"
     CDS             complement(4683..5264)
                     /locus_tag="ACHBBW_001497"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9153181.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHBBW_001498"
     CDS             complement(5836..7128)
                     /locus_tag="ACHBBW_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9153182.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHBBW_001499"
     CDS             complement(7125..7748)
                     /locus_tag="ACHBBW_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153183.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHBBW_001500"
     CDS             complement(7751..8587)
                     /locus_tag="ACHBBW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153184.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHBBW_001501"
     CDS             complement(8604..9491)
                     /locus_tag="ACHBBW_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153185.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHBBW_001502"
     CDS             complement(9507..10790)
                     /locus_tag="ACHBBW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153186.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHBBW_001503"
     CDS             complement(11473..12156)
                     /locus_tag="ACHBBW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9153187.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHBBW_001504"
     CDS             complement(12174..12791)
                     /locus_tag="ACHBBW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9153188.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHBBW_001505"
     CDS             complement(12801..13625)
                     /locus_tag="ACHBBW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9153189.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHBBW_001506"
     CDS             complement(13618..14178)
                     /locus_tag="ACHBBW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153190.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHBBW_001507"
     CDS             14352..15308
                     /locus_tag="ACHBBW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9153191.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHBBW_001508"
     CDS             complement(15613..16569)
                     /locus_tag="ACHBBW_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9153192.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHBBW_001509"
     CDS             16673..17275
                     /locus_tag="ACHBBW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9153193.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHBBW_001510"
     CDS             complement(17363..18862)
                     /locus_tag="ACHBBW_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9153194.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHBBW_001511"
     CDS             complement(18878..19402)
                     /locus_tag="ACHBBW_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9153195.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHBBW_001512"
     CDS             19700..20449
                     /locus_tag="ACHBBW_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9153196.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHBBW_001513"
     CDS             20440..21225
                     /locus_tag="ACHBBW_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9153197.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHBBW_001514"
     CDS             21194..21910
                     /locus_tag="ACHBBW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9153198.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHBBW_001515"
     CDS             complement(22012..23031)
                     /locus_tag="ACHBBW_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9153199.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHBBW_001516"
     CDS             complement(23050..24051)
                     /locus_tag="ACHBBW_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9153200.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 taatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAYYKC010000041        23666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000041 DAYYKC010000000
VERSION     DAYYKC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.041"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHBBW_001517"
     CDS             complement(1..120)
                     /locus_tag="ACHBBW_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9153201.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHBBW_001518"
     CDS             299..1960
                     /locus_tag="ACHBBW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9153202.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="ACHBBW_001519"
     CDS             complement(2030..2392)
                     /locus_tag="ACHBBW_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9153203.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHBBW_001520"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHBBW_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9153204.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACHBBW_001521"
     CDS             3402..4445
                     /locus_tag="ACHBBW_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153205.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACHBBW_001522"
     CDS             4451..5521
                     /locus_tag="ACHBBW_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153206.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACHBBW_001523"
     CDS             5511..6464
                     /locus_tag="ACHBBW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153207.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACHBBW_001524"
     CDS             6476..7486
                     /locus_tag="ACHBBW_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9153208.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="ACHBBW_001525"
     CDS             complement(7771..8592)
                     /locus_tag="ACHBBW_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9153209.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACHBBW_001526"
     CDS             complement(8689..8967)
                     /locus_tag="ACHBBW_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153210.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACHBBW_001527"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACHBBW_001527"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9153211.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACHBBW_001528"
     CDS             complement(11723..12310)
                     /locus_tag="ACHBBW_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153212.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACHBBW_001529"
     CDS             12485..13807
                     /locus_tag="ACHBBW_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9153213.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACHBBW_001530"
     CDS             13962..14738
                     /locus_tag="ACHBBW_001530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9153214.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHBBW_001531"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHBBW_001531"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9153215.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHBBW_001532"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHBBW_001532"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9153216.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACHBBW_001533"
     CDS             16628..17257
                     /locus_tag="ACHBBW_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153217.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACHBBW_001534"
     CDS             complement(17381..18490)
                     /locus_tag="ACHBBW_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153218.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHBBW_001535"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHBBW_001535"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9153219.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19308..19406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19444..19980
                     /locus_tag="ACHBBW_001536"
     CDS             19444..19980
                     /locus_tag="ACHBBW_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9153220.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="ACHBBW_001537"
     CDS             complement(20020..21783)
                     /locus_tag="ACHBBW_001537"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153221.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="ACHBBW_001538"
     CDS             complement(21786..23501)
                     /locus_tag="ACHBBW_001538"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153222.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7633 a         4322 c         4801 g         6910 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaa
//
LOCUS       DAYYKC010000042        23524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000042 DAYYKC010000000
VERSION     DAYYKC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.042"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1387
                     /locus_tag="ACHBBW_001539"
     CDS             47..1387
                     /locus_tag="ACHBBW_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153223.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ACHBBW_001540"
     CDS             1605..2048
                     /locus_tag="ACHBBW_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9153224.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACHBBW_001541"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACHBBW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9153225.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ACHBBW_001542"
     CDS             complement(4373..5596)
                     /locus_tag="ACHBBW_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9153226.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ACHBBW_001543"
     CDS             complement(5627..6466)
                     /locus_tag="ACHBBW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9153227.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ACHBBW_001544"
     CDS             complement(6483..7163)
                     /locus_tag="ACHBBW_001544"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9153228.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ACHBBW_001545"
     CDS             complement(7165..8238)
                     /locus_tag="ACHBBW_001545"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9153229.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8885..9556)
                     /locus_tag="ACHBBW_001546"
     CDS             complement(8885..9556)
                     /locus_tag="ACHBBW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9153230.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACHBBW_001547"
     CDS             complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACHBBW_001547"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9153231.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12633..12807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12980..13417
                     /gene="ndk"
                     /locus_tag="ACHBBW_001548"
     CDS             12980..13417
                     /gene="ndk"
                     /locus_tag="ACHBBW_001548"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9153232.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13439..14305)
                     /locus_tag="ACHBBW_001549"
     CDS             complement(13439..14305)
                     /locus_tag="ACHBBW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9153233.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14465..16558)
                     /locus_tag="ACHBBW_001550"
     CDS             complement(14465..16558)
                     /locus_tag="ACHBBW_001550"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9153234.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17307..17930)
                     /locus_tag="ACHBBW_001551"
     CDS             complement(17307..17930)
                     /locus_tag="ACHBBW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153235.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18089..19486)
                     /locus_tag="ACHBBW_001552"
     CDS             complement(18089..19486)
                     /locus_tag="ACHBBW_001552"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9153236.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19518..20489)
                     /locus_tag="ACHBBW_001553"
     CDS             complement(19518..20489)
                     /locus_tag="ACHBBW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153237.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20863..21288
                     /locus_tag="ACHBBW_001554"
     CDS             20863..21288
                     /locus_tag="ACHBBW_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153238.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21776..22477)
                     /locus_tag="ACHBBW_001555"
     CDS             complement(21776..22477)
                     /locus_tag="ACHBBW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9153239.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22493..22933)
                     /locus_tag="ACHBBW_001556"
     CDS             complement(22493..22933)
                     /locus_tag="ACHBBW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9153240.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22933..23508)
                     /locus_tag="ACHBBW_001557"
     CDS             complement(22933..23508)
                     /locus_tag="ACHBBW_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9153241.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6765 a         4886 c         4051 g         7822 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
     8881 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
     8941 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
     9001 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
     9061 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
     9121 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
     9181 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
     9241 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
     9301 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
     9361 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
     9421 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
     9481 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
     9541 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
     9601 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
     9661 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
     9721 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact
     9781 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
     9841 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
     9901 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
     9961 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    10021 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    10081 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    10141 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    10201 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    10261 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    10321 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    10381 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    10441 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    10501 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    10561 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    10621 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    10681 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    10741 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    10801 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    10861 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    10921 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt
    10981 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    11041 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    11101 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    11161 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    11221 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    11281 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    11341 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    11401 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    11461 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    11521 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    11581 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    11641 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    11701 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    11761 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    11821 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    11881 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    11941 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    12001 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    12061 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    12121 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    12181 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    12241 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    12301 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    12361 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12421 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12481 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    12541 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    12601 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    12661 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    12721 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    12781 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    12841 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    12901 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    12961 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    13021 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    13081 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    13141 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    13201 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    13261 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    13321 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    13381 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    13441 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    13501 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    13561 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    13621 ttctttcttt atgtgataag ctccgtttca tatcttcatt cacactacgt gcctttttca
    13681 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    13741 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    13801 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    13861 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    13921 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    13981 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    14041 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    14101 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    14161 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    14221 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    14281 tcttgatcaa ttcaccatca tacataatag ttctccttat taatttaaat ttttccacat
    14341 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    14401 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    14461 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    14521 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    14581 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    14641 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14701 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    14761 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14821 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    14881 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14941 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    15001 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    15061 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    15121 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15181 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    15241 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15301 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    15361 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    15421 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    15481 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    15541 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    15601 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    15661 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15721 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    15781 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    15841 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    15901 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    15961 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    16021 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    16081 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    16141 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    16201 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    16261 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16321 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16381 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    16441 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16501 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16561 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16621 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    16681 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    16741 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    16801 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    16861 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    16921 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    16981 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    17041 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    17101 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    17161 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct
    17221 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
    17281 aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat
    17341 gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg
    17401 ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc
    17461 ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct
    17521 actttttcag aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca
    17581 tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa
    17641 atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa
    17701 taaatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa
    17761 gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt
    17821 tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt
    17881 aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc
    17941 actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa
    18001 gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt
    18061 tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt
    18121 ctttgaacca atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg
    18181 aaataaagcc atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc
    18241 acatatgata gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat
    18301 gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc
    18361 gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat
    18421 gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat
    18481 aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac
    18541 cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga
    18601 tacctaaaag atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag
    18661 gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag
    18721 ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt
    18781 cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac
    18841 caccttgctc catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat
    18901 agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat
    18961 aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac
    19021 caaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca
    19081 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg
    19141 gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt
    19201 cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg
    19261 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg
    19321 aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat
    19381 caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg
    19441 ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt
    19501 ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt
    19561 tcattttctt cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg
    19621 attttatctg ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct
    19681 aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa
    19741 cattcgatgg caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt
    19801 tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc
    19861 tctaattgga tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt
    19921 gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca
    19981 tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct
    20041 gctggtcctt ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca
    20101 aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc
    20161 aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag
    20221 ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg
    20281 ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta
    20341 gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc
    20401 ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc
    20461 atcttagcca catcacggat cgatgccata cattctcact cctgccagtt acatttgatt
    20521 tctctttctt gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca
    20581 atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa
    20641 acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt
    20701 tttaaaacgc tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt
    20761 tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc
    20821 caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta
    20881 ctatttattt tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct
    20941 gaccaatcct cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc
    21001 accaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg
    21061 acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct
    21121 tctgaatcat ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac
    21181 gaatatatta tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc
    21241 ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg
    21301 gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa
    21361 ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat
    21421 ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt
    21481 caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag
    21541 aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc
    21601 ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat
    21661 caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa
    21721 ggcttattct acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc
    21781 ctaattcttg accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt
    21841 ccaattcagc taatttttct tggatctgac ggatcacctt ttttgcatct tctggatttt
    21901 ctacaaaatc gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat
    21961 accattgatt atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt
    22021 ctagctgttc atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa
    22081 ctttgatatg gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta
    22141 gcatcgtatc taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat
    22201 cagcgtttag atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat
    22261 cgttcactaa agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata
    22321 aaaaagcgta ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat
    22381 tttcataaaa tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac
    22441 ctgcaccaat cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt
    22501 actcgcctac gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt
    22561 ctgattttaa atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt
    22621 cttgtacaat gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc
    22681 ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca
    22741 gttcttccat atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat
    22801 tgctttcttg gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg
    22861 ctttttcgat acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat
    22921 atatttgact cattattttg cctccttcaa taataactga tttttaatag atactgctcg
    22981 taattcattg accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt
    23041 atccataaaa tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc
    23101 gccaatattc aatattccga ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag
    23161 aagttgtgag aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc
    23221 cttttcaaat aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac
    23281 atcttttcca ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc
    23341 gttattttcc agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta
    23401 aaccagttcg atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta
    23461 actttcttta ttaccaaaac gggaaccgca aaaaacagta agtttcataa aaaaacactc
    23521 ccta
//
LOCUS       DAYYKC010000043        23158 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000043 DAYYKC010000000
VERSION     DAYYKC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.043"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..541
                     /locus_tag="ACHBBW_001558"
     CDS             20..541
                     /locus_tag="ACHBBW_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153242.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            638..859
                     /locus_tag="ACHBBW_001559"
     CDS             638..859
                     /locus_tag="ACHBBW_001559"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9153243.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1016..1180)
                     /locus_tag="ACHBBW_001560"
     CDS             complement(1016..1180)
                     /locus_tag="ACHBBW_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM9153244.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1283..1804
                     /locus_tag="ACHBBW_001561"
     CDS             1283..1804
                     /locus_tag="ACHBBW_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9153245.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1791..2231
                     /locus_tag="ACHBBW_001562"
                     /pseudo
     CDS             1791..2231
                     /locus_tag="ACHBBW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2271..2429)
                     /locus_tag="ACHBBW_001563"
     CDS             complement(2271..2429)
                     /locus_tag="ACHBBW_001563"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM9153246.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2522..3283
                     /locus_tag="ACHBBW_001564"
     CDS             2522..3283
                     /locus_tag="ACHBBW_001564"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9153247.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3470..4822
                     /locus_tag="ACHBBW_001565"
                     /pseudo
     CDS             3470..4822
                     /locus_tag="ACHBBW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4838..6058
                     /locus_tag="ACHBBW_001566"
     CDS             4838..6058
                     /locus_tag="ACHBBW_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9153248.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6093..6770
                     /locus_tag="ACHBBW_001567"
     CDS             6093..6770
                     /locus_tag="ACHBBW_001567"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9153249.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6767..7963
                     /locus_tag="ACHBBW_001568"
     CDS             6767..7963
                     /locus_tag="ACHBBW_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9153250.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7978..8301
                     /locus_tag="ACHBBW_001569"
     CDS             7978..8301
                     /locus_tag="ACHBBW_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9153251.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8305..8640
                     /locus_tag="ACHBBW_001570"
     CDS             8305..8640
                     /locus_tag="ACHBBW_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9153252.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8637..9032
                     /locus_tag="ACHBBW_001571"
     CDS             8637..9032
                     /locus_tag="ACHBBW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9153253.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9029..9400
                     /locus_tag="ACHBBW_001572"
     CDS             9029..9400
                     /locus_tag="ACHBBW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153254.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9416..9976
                     /locus_tag="ACHBBW_001573"
     CDS             9416..9976
                     /locus_tag="ACHBBW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9153255.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10103..10414
                     /locus_tag="ACHBBW_001574"
     CDS             10103..10414
                     /locus_tag="ACHBBW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153256.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10438..10629
                     /locus_tag="ACHBBW_001575"
     CDS             10438..10629
                     /locus_tag="ACHBBW_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153257.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10629..14474
                     /locus_tag="ACHBBW_001576"
     CDS             10629..14474
                     /locus_tag="ACHBBW_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM9153258.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14475..15347
                     /locus_tag="ACHBBW_001577"
     CDS             14475..15347
                     /locus_tag="ACHBBW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM9153259.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15344..16393
                     /locus_tag="ACHBBW_001578"
     CDS             15344..16393
                     /locus_tag="ACHBBW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153260.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16397..17221
                     /locus_tag="ACHBBW_001579"
     CDS             16397..17221
                     /locus_tag="ACHBBW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153261.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17239..19371
                     /locus_tag="ACHBBW_001580"
     CDS             17239..19371
                     /locus_tag="ACHBBW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9153262.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19388..19537
                     /locus_tag="ACHBBW_001581"
     CDS             19388..19537
                     /locus_tag="ACHBBW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153263.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19534..19980
                     /locus_tag="ACHBBW_001582"
     CDS             19534..19980
                     /locus_tag="ACHBBW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153264.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19982..20119
                     /locus_tag="ACHBBW_001583"
     CDS             19982..20119
                     /locus_tag="ACHBBW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9153265.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20156..20449
                     /locus_tag="ACHBBW_001584"
     CDS             20156..20449
                     /locus_tag="ACHBBW_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153266.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20472..20669
                     /locus_tag="ACHBBW_001585"
     CDS             20472..20669
                     /locus_tag="ACHBBW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9153267.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20680..21699
                     /locus_tag="ACHBBW_001586"
     CDS             20680..21699
                     /locus_tag="ACHBBW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9153268.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21956..22603
                     /locus_tag="ACHBBW_001587"
     CDS             21956..22603
                     /locus_tag="ACHBBW_001587"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9153269.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22928..23074)
                     /locus_tag="ACHBBW_001588"
                     /pseudo
     CDS             complement(22928..23074)
                     /locus_tag="ACHBBW_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         8052 a         3925 c         5103 g         6078 t
ORIGIN      
        1 ggattaagga ggaagagtaa tgcatatagg agataagata cataaattga gacggcttaa
       61 taacgaaaca atgaacgact tagttaaaca aaccggactg tcaaaatcat gggtatctga
      121 tattgagtct ggaaaaaagc aacgcgctga ctttaataag atatgtgcaa tagccaaaca
      181 ttataaagtt agcttagaat actttcagga tgattatttg gatagtgatg aacacgagga
      241 tattattcct ttgacagacg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
      301 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
      361 ctcagtacca gaggtttatt acaaaggtca gaagttagag ggattgatag atgtaagcta
      421 tcactaccat acaacaactg atgtggtagc tggttcaaca aacgacatca atatggagta
      481 cctaggtgaa gtaactaagt atccagatat aaaaacaatc ggacacaaga gagatgtgta
      541 aatgaaagaa gctagaccta gagacgagat agacaaacta tacaagacca aacgatggcg
      601 agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt gcaagcgtcg
      661 agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg aagacctgtc
      721 actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca acagagagca
      781 tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta
      841 ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac tctaaaagat
      901 taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca
      961 agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga cgaagctatt
     1021 tcttagtcca tttgtttcct ttttgagaag taggaggtaa tcggtcgcct ggatcaattt
     1081 ttacttctcg tccgccttgg acatttccac cacgaggtcc cacttcttta taggttcctt
     1141 ttggtttatt gtcttcgccg ggtttataga gttctcccat aggaatccct ccctaaaaaa
     1201 atttcggcac agcactgccg ataacttaat tataaggatt gtgataacga tttcaatata
     1261 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
     1321 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
     1381 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
     1441 ggataccttt gagtttatcg ctgatgaatt gctgtctgtg gatttaatca gtaatccgga
     1501 cgtccataca atggctctct actccaattg gtattcgcaa tacgtttctt tagaaaaaca
     1561 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
     1621 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
     1681 gatggataca gcgtcgcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
     1741 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
     1801 ctaatatttt ggatatgtcc tacacagaac gtgtggacta ttggcaaagc tatcttgagg
     1861 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
     1921 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
     1981 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
     2041 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
     2101 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
     2161 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
     2221 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
     2281 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
     2341 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
     2401 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
     2461 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
     2521 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
     2581 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
     2641 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
     2701 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
     2761 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
     2821 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
     2881 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
     2941 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
     3001 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
     3061 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
     3121 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
     3181 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
     3241 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
     3301 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
     3361 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
     3421 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
     3481 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
     3541 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
     3601 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
     3661 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
     3721 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
     3781 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
     3841 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
     3901 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
     3961 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
     4021 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
     4081 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
     4141 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
     4201 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
     4261 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
     4321 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
     4381 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
     4441 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
     4501 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
     4561 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
     4621 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
     4681 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
     4741 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
     4801 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
     4861 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
     4921 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
     4981 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgcgg agtttggcca
     5041 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
     5101 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
     5161 tttccgaaat atcaatggag aagtagcaga tatggcgtta ataaaacctg gtgcttgcca
     5221 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
     5281 cagttataag caagttattt atgtacatta catggaaatg ctccacttta aacaaccgag
     5341 gtttggtggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
     5401 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga ggtaatgaag ggctaaaagt
     5461 taagtttgct agcaatgtgg atgaagaagc taaaaaagct acagttaaaa acattgctga
     5521 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
     5581 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
     5641 cgcgatggtc tacaacgtgc cggaacattt cattgggagt aaccagtcga gttactcttc
     5701 acaggaacag ctcaatatgg agtttttgac atacaatcta gtaccgaccg ttaatcaata
     5761 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
     5821 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaagggggc aattctatca
     5881 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
     5941 accgataaat aaaaccggta tggatgatta tcatatttcc ggagacctat atccaatcga
     6001 tatggatcca acattaagaa aaacaaactc gtctaaaagc gtagccgaaa acggttaggc
     6061 ttttttagtt tgcaccgaag ggaggtgaaa ggatgaaaaa agtgacgtta agcggcgatg
     6121 tcgtggataa cgataccgcg tggttatatg actggtttgg gattgattgt atctcaccag
     6181 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
     6241 ctaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
     6301 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgtg
     6361 cttgcgaacc gcttagaatc tcgccaacag cacacatcat gattcacaac gcatgggtga
     6421 ccactagcgg caacgccgag gaattaaaag ccaatgcgga aatgttaagc agtgtggatg
     6481 agtctatcgt taatgcttac gagatcaaga caggactcga ccggaaaaag cttgctgatt
     6541 taatggcaaa agatacttgg ttaaacgcac aaacagcagt ggcggaaggt tttgcggatg
     6601 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
     6661 aaaacgcggt aactaagttg aaaaatctaa tactcaaagc ggaaacaccg caagaagaaa
     6721 cgctcttgca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
     6781 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gatttaaacg cgcaactcaa
     6841 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
     6901 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
     6961 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
     7021 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
     7081 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
     7141 gactattcat actttggttc gtcagtttga ctctttgcaa gaatacgtaa acgttgaaaa
     7201 agtgaccact actagcggtt ctcgggttta tgaaaaatgg tctgatatta acccactgac
     7261 cgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
     7321 caaatacttg atcaaacgct acgcaggtat ttctacagta actaacagct tgctaaaaga
     7381 tactgccgaa aacattttgg catggttgtc taactggatc gcgaaaaaag tagttgttac
     7441 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcagcgcaaa agaaagatgt
     7501 tacagatgtt gatgggatta aagatatcat aaacgtccaa cttgatccag ctatcgaagc
     7561 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
     7621 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
     7681 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
     7741 taaatatcca ctgtatattg gtgatctgaa agaagctgtt acattgtatg atcgcgaaaa
     7801 catgagcttg ctgacaacga atattggcgg gggggctttt gaaacagaca ccaccaaagt
     7861 acgggttatc gatcgatttg atgtgcagtt agtcgatgat gaagcagttg ttttagctac
     7921 atttacatct atcgcaaacg aagtgccagt aacaaacggt taaggagctg atttcttatg
     7981 attcttgatc ctaaaatgga tttagacgaa atcaaaaacg cactaaagat tgataccgat
     8041 gatgacgatg tggaagtaag acgtgcggtg caagctgcaa ttgcatacat tagaggggct
     8101 atcgggaatg ataagccctc tttttataag caagaaaatg acacggttga tctgattaat
     8161 ttagctattc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
     8221 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagatat
     8281 ttgctttttg tggaggagta gcgtatgccc cttattcaaa caggaaattt aaatcaacgc
     8341 atcaagtttg tccgagatac gacggtcaag gacgaggacg ggcaagttgt ccggacttct
     8401 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
     8461 tccattggta cggctttgga aggaacactg acgttcatta tccgctatca acaaaaatca
     8521 gagctaacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
     8581 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
     8641 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
     8701 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa aagttacaac
     8761 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
     8821 aacatgctaa ggaccatgtg gttacaagct cggtaaaaaa cggattgata gaagtcggct
     8881 atgataaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
     8941 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
     9001 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
     9061 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
     9121 ggctcaaaca gtacctagcc ttccagttgg cagaattaca gagatattcg gcaactatga
     9181 agatttcgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
     9241 atcgacctta aaagaggttg atgcctttta ttttgccctt gatgagatta tgagggggaa
     9301 tggttggcaa tgcgcataca cggaacaaac agatgatgag gatttagaag gtgcaaagcg
     9361 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
     9421 aacagtagga tttgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
     9481 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
     9541 cgcaacttct aacacaacat acgcttcaaa cgtaccattc ttcattgcaa gtaaaggggt
     9601 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
     9661 aatcattggc gctaaaaccg tggatggtgt aaatgtaatt ggttcagaaa ctgaagcgcc
     9721 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttat tcatgggatt
     9781 ggcaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggagt
     9841 tgaattgcaa accgattcta tcgaagggga attcatttct gatgaacgtg gctatgtata
     9901 cttaacagcc gtagaatcag aagaaatgac cttacaaaaa ttcaaggact tggtaaataa
     9961 caaagcgggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    10021 gaaccagaac caaaaattga tacacaaggt tagccatttt ttggctagcc ttattttttt
    10081 gtaaaaacaa ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa
    10141 aacattcaaa caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga
    10201 gaaagcggaa aaagcagacc taactgattt agaagcaatt gattcaatga ttgcaattac
    10261 agcagatatt ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc
    10321 gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa atcaatgaac tgggaaacaa
    10381 tgaaaaaaag cagacggcga gcaaaaagag ataagttttt ctgaagctag gaaagcaatg
    10441 gatcaaatct acaaagattt aatcgaatca ggttggacga tgagagatgt ggacgaagcc
    10501 gactatcatt atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat
    10561 ggtgctgatt tcatcaaaca atttttatcg gctgaagact tggtaaaact tgaggaagga
    10621 ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag
    10681 atagcactaa ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt
    10741 cgaatatgcg tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta
    10801 gtcaaaagac gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat
    10861 taacaaagcg ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta
    10921 accttaacac acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga
    10981 atgacacaaa aaaagaattg gtgtattcca aaacagccgt caatgattta tctaacgaaa
    11041 tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg
    11101 aatctggtgc gtttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga
    11161 aagctatcga agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag
    11221 caaatgaaac cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat
    11281 ctagcaggca attagaagca ctcaaaagct ccagcgatca atcaggaaaa aaaatagaag
    11341 attttggcga caagtccaca aggtcagcta ggaaactgga tgggctaaaa gacaaattag
    11401 gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta
    11461 gcagtgtagt aagtggcgtg caaggcttgg ttggcgaagc agtaaacgca tcggattcat
    11521 tgatgaagtt ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct
    11581 cgaaaaaaga aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga
    11641 acacaaccgc acaattggca tctaacggga ttcctaacta tacagaacta accaaggcgg
    11701 caggtaactt gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga
    11761 tgctaacgca gacggcagga gctgggaaac taacaactga aaactggaat caattagcag
    11821 atgcgatacc gggcgcttca gggctgttac aagacgctat gttgaaaaac ggagcttata
    11881 caggaaactt ccgtgatgca atggcgcaag gtcaaatcac ttctgatgag tttaaccaag
    11941 cgattgtaca gttaggtatg aatgacggag cagttaaagc agctacttcc acagatacat
    12001 tgagtggttc ttgggaacag atgaaatcta ctgtaataaa tgggttacaa agtatcatag
    12061 acaaagtagg cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa
    12121 attctattcc taaaattact caatttatag gttggttgag agatattgga acgtggatcg
    12181 ttgaaaatag agagccacta acatggattg tcggaatcat aggcggaatt acattagcag
    12241 taaaagcatt gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc
    12301 cttttgtggc gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata
    12361 caaaatctga aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag
    12421 ttatgagcgt tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt
    12481 ggctttggga aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga
    12541 agaacagcgt tgtgaatacg gttcagaacc tatggaatgg tgtgaaaaac ttcttcagta
    12601 atggcgttgg agacacttgg aataaggtag ttggctgggt aaaaaacatt ttcaacaaag
    12661 caactgaatt gaagaacaaa gtttctgatg caattggtaa cctgtggaac ggtatcaaag
    12721 acacattccg tagaggcatt gatacggtat tcaattggtt ttcagaacta ccgacgaaga
    12781 tgaagaatgc cattattggc ggtaaaaatg ccattgttga tgcgttcaaa agtattttca
    12841 acgcagcact taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg
    12901 tactagaaaa actgggtgct gacaaactcg aagaatggaa agtgccacaa tacgcaaaag
    12961 gaacaccaaa cggaggtcat ccgggcgggc ctatgatggt aaatgacggt agaggtgctg
    13021 aagcggtaat cacacctaac ggacaagcat ttatcccacg agggcgaaat gtagtgttga
    13081 atgcaccaaa aggcacacac gttctaacag ctgaagaaac agcttatatg actggaaaca
    13141 aagcaccaag atatagatac gccaaaggta caggcttttt cggaaatcta tggaacaacg
    13201 tcaaaggatt tgctggagat gttggaaaca agctgaaaga tgtagtcggc gatgtatggg
    13261 attttgtaac agacccggga gcgttggcta ggaaagtgtt aaatggtctt ggcgtactgg
    13321 aagggcttgt caaatatcct ttagatgttg gtaaaggtat tctaagcaag gctaccgaag
    13381 cattgacgaa caaaatcaca gaactattca gcagtggcag tttagacact tcaatgggca
    13441 tgcaaggcgt ttacaaatac ttggcggacg ttgcagttgc agtaatgaag aagtttccag
    13501 gctttgtggc aactagtggg tatagaccag gtgaccccta ttcgcatggt aaacgtaatg
    13561 ccattgatat tgcactacct ggtgtcacag gaggctcgcc acgctacaca gaagcagcca
    13621 attacgcatt tgagaagttt gcaaacaaaa tcggctatgt tatcacaaat ggcaaggttc
    13681 gtgaccgttc aggacaatca ggtacaggta ttcacaatga ttggagacca tggcccgatg
    13741 gagatcacta tgatcatgtg catttaaacg gtgtgaaaga cccacaaaac actcaaattt
    13801 caggagatag cgtgggaggc agtggggtag aaagatggcg caatgtagca attagagcgt
    13861 tgaaaatgac cggtcaatac agtactgcaa acttaaatgc attactaaat caaatgcgta
    13921 cagagtcaaa tggtaatcct aatgcagtta acaattggga tattaacgcc aaaaatggaa
    13981 caccatcaaa agggttgctc caagtgattg acccaacatt cagacagtat gcaatgccag
    14041 gattcaacag caatattttt gacccactat ctaacatctt agcttcaatc agatacgcac
    14101 tatcaagata tggctcacta acaaatgcct atcgtggagt tggttacgca aacggtggaa
    14161 ttgtaaacca acatcaaatt gcggaaatcg cagaaggaaa caagccagaa attattattc
    14221 ctttagataa ggctaaacga tcaagagcga tgcaattatt gtctatcgca atggacaaac
    14281 taggagtttc tcctaaaact tatggaaatg cgacaactgt ttccagtgat tacgaaatcc
    14341 tagaagccat tgaaaaacag gcggcaacaa cgaatcagct gttatcgtta ttgctggcat
    14401 ttttcaaagg aaatagccga actgatagag atttagcttt agatattcag aagatcttgg
    14461 ttaggaggat gtagatgcga actgtattac tgagaaatag aataaacgaa gaaattgatt
    14521 tgtctacgga ggattatttt gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag
    14581 aacatgtggg acagtggggg aatttcagag aaagcagtga gaacgttgaa atatctgaat
    14641 ttcagtcatc tgtaatcatt tctgtgcatg ggtttcgaga aaaagaactg tacaattcac
    14701 ttgtgcagtt cctatcggaa ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa
    14761 tggtaagaag atgcagtcta aaatctttaa gtaagactga aatcgatccg aaaacatcgt
    14821 tactaacaga cactttagaa ctatatttca catcaaactg gtattccgta aaacgcgaaa
    14881 aattaattca gcgaccgaat gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat
    14941 cttatatcta cactcaaaac ttgtgggaga aaaaaggtgt attcaaattc aataataatt
    15001 ctgtatacct aacgaatagc aaggaacgga tgtccccgct aaaaattcgt gtgattggga
    15061 agtgttcaaa tccgtattgg gaagtaatcc aaaactcaca aatcatcgca acggatggat
    15121 acttcataga tatgactgaa acacagactc tagaagtatc aagccttttt gaagatacaa
    15181 cggcgatttt aaaagatatt gcaggggtag aatcttctgt ctaccaacaa caggattata
    15241 caaaaactaa ttttgttcag gccccgactg gagaattcag cattgtgttt catgttgggg
    15301 gggcagacgt agagattgag ttatacgagg agcgtgatct gttttgattt tagcagtcac
    15361 gctttttcat cgtgatttaa tgctttataa cgaacattta ttctctaaca gttttgaatt
    15421 tggtgcggac gagatcaacg aagaggctag tagctttacg atggataagt acgttcctgt
    15481 aaaaacaggc gattttctac tagcaaaata tattcctagt ggaaaatttg catattttgg
    15541 tgtaattacg tcgcaggaag acgaaaaaat cagttgtaaa agcttactta gtttagctga
    15601 tagtgagata ccgactgcac gtgtgtcagg agataactac gaagagcata tccggcggtt
    15661 gattgaatac tatttgctga atgatccaac gaaacaacta aaagatattt tagacgtcaa
    15721 agcagaaagt gcgacctctc attcgtatca agctaccgat acgaataaac acaagttaag
    15781 tgcgtatatt ctcaatggtt tcaaaaaata caacgtaaaa tggtatttca agggaattca
    15841 aaacagaaaa atctatacag gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa
    15901 ttcttctgaa tttagcgatt gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa
    15961 caagctatta atcgttgata aagcaatgaa agatatagag aaaccgataa tactgtcaac
    16021 atggtatttg gacgaagaaa acaatttgac acaagatgga tcgaaagaaa atatcacgaa
    16081 accaactgtc aatttggtta atatctacga tcaaaccgca gaagataaag catcctacga
    16141 agatgtggca aaatcagagt tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt
    16201 tgtaagaaat gcaaaaaatt tgaatgtcga aacgattgaa acagggatgt ttgccacgat
    16261 ttcttataaa ggaaagatat acaagtcggt tttgacagct tggcgaatat caagtgataa
    16321 ggagtttgtg gaattgactt tcggaaacat aagaagtcgt tttatggatt attttgaaga
    16381 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    16441 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    16501 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    16561 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    16621 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    16681 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    16741 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    16801 actatgctca gttacttcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    16861 cgagctgact ctctctaaag gagaaatttt gtggagtggg actgcgttaa tgcatgatac
    16921 tcaaactgtc caaccttcaa aaaaaatgtg gcagacagtt tcaggttttt tattagtatg
    16981 gctcccatac gaaaacgggc aagcaattgg ggatagatat gtaactacgc ctttttataa
    17041 agagcgtgta acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    17101 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    17161 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    17221 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    17281 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    17341 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    17401 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    17461 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    17521 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    17581 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    17641 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    17701 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    17761 gaatcagata agcaagtaaa tgatcgtatt gataagacaa atgatcgtat tgatcaaacc
    17821 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttc
    17881 ggggactatg attatagtgg gaatccgaac cttagcgcta aacttaatgc ttcaagtttt
    17941 tctttaggta ctggcgcaac agtcgcggat gataatgatg agattgtgtt taccttagat
    18001 ggcacaaatc aactatcgaa gtacgctagc aaagcgcaag tgccggtagc agaagggaaa
    18061 caatatacga ttagttgtga aattatgtta gaagatgggt tcactggcga cccttcagga
    18121 atacgtctgc aacacgcata tttgccaggc ggggttgttg ttttacagac agagacagta
    18181 cctaaaaatg aattgaatac gtggcagacg cttatcggaa cgcgaacggt aaaatacacc
    18241 tctgacgtac caaatgaatg gtaccctttg tttagagaca tccgaacact taaaccgacc
    18301 ggtaaagtta gactaagaaa aattaagatt gaagaaggct caacagccac cccatatcag
    18361 ccaaacttac ttgatgaccc ttactggcta ggtaaagcgc ctttgggtga gaatattgct
    18421 aataagtctg ttacgtttcc aatcaaatct agcgcctaca gcctatacca agctaacatg
    18481 gaagaagaat ttgttttagg tcaaacctat acaattacca tgaaggcaac caaaccacct
    18541 atccaaacgt tcattgttta caacgaggat agtggagatt atagatatgg taacttagag
    18601 ccagtagaag ggttagttga tacatggggt ctgactttta caccgcagaa ggttggagtt
    18661 aactacccca aacggttaac tattatccag tatccccaat caacaacagg tgcatgtcaa
    18721 attgactggc tcaagatcga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    18781 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    18841 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    18901 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    18961 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    19021 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    19081 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    19141 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    19201 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19261 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    19321 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    19381 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    19441 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttctaaattg
    19501 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    19561 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    19621 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    19681 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    19741 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    19801 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    19861 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    19921 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    19981 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20041 agttcgacta ttcgttccac tatgcattac agacgaagaa gcagataaaa tcattagcaa
    20101 agaagaaagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    20161 caatttaggg gaatggggaa caatcgcagg atcaattact gcgattgttt ctttgatttt
    20221 attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt
    20281 aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    20341 gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt
    20401 agaacacaca caacaaatta aaactttatt tagagaaagg tctcggtaaa aaagaaagga
    20461 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa
    20521 cagtattgcc agctttatct gtattagtag ccacattagg caaagcatat ggatggaatg
    20581 gaacagatat ggcagttctg actatcaatg cagcagcaac atttttaggt gttatcactg
    20641 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    20701 actgcgatga gcctattaac ggctcttttt ttattgccga ttaatgggtt tgcctatacg
    20761 attaacaatg aatttaattt aggtgtaaac gaaggtagtt ctcaagtagc aaataatcag
    20821 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    20881 atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt
    20941 tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat
    21001 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    21061 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
    21121 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
    21181 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    21241 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    21301 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
    21361 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
    21421 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
    21481 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
    21541 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
    21601 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    21661 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag
    21721 gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa
    21781 tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc
    21841 atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa
    21901 atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc
    21961 taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa
    22021 cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc
    22081 gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc
    22141 tgtaggaaaa atattagaag ttgctagtat taaacaattt ataagccaag ctgataaatt
    22201 aaaagagaaa cttaggataa ttattttact ttcattaaat agaggcatca ttgatgaaag
    22261 cgatttgaat agttttatta aagatattga agctaaaggt gaaaagcttt cagtggaaga
    22321 agtagaaaaa cgagctgtaa aacataattt ggtttgcgag acaagggaac aagtaattaa
    22381 agaaataaaa tcattatctg aagatatggt aaaatatcag aaggtgttta attgctacaa
    22441 gaaatataca caagaatcct atgtaatgcc acttaatatc caaacgatta gtaaaaatag
    22501 aattagaaga attaacaagt ttgatccttc tggaaatatg aaggcacctg gaaatgtcct
    22561 caatgaagta gatgaaagtt taaaaaaata ttttacaaag taaacgatat ttttgttgac
    22621 atttttctgt tcgttagtta ccatgtagtt atagaaggac gtaagcgcct atttttaatg
    22681 tacacatcgg gacgtatgcg cccacataga aacatagttg agagacatct atatggtgtc
    22741 tcttttttta ttaatatgca aaaaagtcta gttttttcaa taatatttat gtaataataa
    22801 attagccacc acaacaaaga atgaaatcca ttattatcta gtctagtcca ttctttttgt
    22861 ttgcagtatt tgtgatggct ttccgtaccc ttagctcagt tggtcagagc agacggctca
    22921 taaccgtccg gtcgtacttc tcgtaatcca ggaaggccgt cagctccgtc cgcagtagcg
    22981 tgtttacggc cagctccaga tgggagcgga ataactcgtt caaatcacct ttggtggcta
    23041 gagtctgcag gagttctgta ataaaatcgt tcatagggaa gtcctctttt ctgtgaatgg
    23101 gtgtggtaac tttattctac agaagggact tccttttttc tatttacaca aaatattt
//
LOCUS       DAYYKC010000044        23028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000044 DAYYKC010000000
VERSION     DAYYKC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.044"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..566
                     /locus_tag="ACHBBW_001589"
     CDS             84..566
                     /locus_tag="ACHBBW_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153270.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            763..1653
                     /locus_tag="ACHBBW_001590"
     CDS             763..1653
                     /locus_tag="ACHBBW_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM9153271.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1979..4453
                     /locus_tag="ACHBBW_001591"
     CDS             1979..4453
                     /locus_tag="ACHBBW_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9153272.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4556..6292)
                     /locus_tag="ACHBBW_001592"
     CDS             complement(4556..6292)
                     /locus_tag="ACHBBW_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9153273.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6289..6993)
                     /locus_tag="ACHBBW_001593"
     CDS             complement(6289..6993)
                     /locus_tag="ACHBBW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9153274.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7124..7627
                     /locus_tag="ACHBBW_001594"
     CDS             7124..7627
                     /locus_tag="ACHBBW_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9153275.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7587..7925
                     /locus_tag="ACHBBW_001595"
     CDS             7587..7925
                     /locus_tag="ACHBBW_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9153276.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7946..9364
                     /gene="ffh"
                     /locus_tag="ACHBBW_001596"
     CDS             7946..9364
                     /gene="ffh"
                     /locus_tag="ACHBBW_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9153277.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9476..10054
                     /locus_tag="ACHBBW_001597"
     CDS             9476..10054
                     /locus_tag="ACHBBW_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9153278.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10058..10579
                     /locus_tag="ACHBBW_001598"
     CDS             10058..10579
                     /locus_tag="ACHBBW_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9153279.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10658..11212)
                     /locus_tag="ACHBBW_001599"
     CDS             complement(10658..11212)
                     /locus_tag="ACHBBW_001599"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9153280.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11465..11740
                     /gene="rpsP"
                     /locus_tag="ACHBBW_001600"
     CDS             11465..11740
                     /gene="rpsP"
                     /locus_tag="ACHBBW_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9153281.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11752..12003
                     /locus_tag="ACHBBW_001601"
     CDS             11752..12003
                     /locus_tag="ACHBBW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9153282.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12127..12918
                     /locus_tag="ACHBBW_001602"
     CDS             12127..12918
                     /locus_tag="ACHBBW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153283.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13088..13609
                     /gene="rimM"
                     /locus_tag="ACHBBW_001603"
     CDS             13088..13609
                     /gene="rimM"
                     /locus_tag="ACHBBW_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9153284.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13599..14360
                     /gene="trmD"
                     /locus_tag="ACHBBW_001604"
     CDS             13599..14360
                     /gene="trmD"
                     /locus_tag="ACHBBW_001604"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9153285.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14496..14843
                     /gene="rplS"
                     /locus_tag="ACHBBW_001605"
     CDS             14496..14843
                     /gene="rplS"
                     /locus_tag="ACHBBW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9153286.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15153..15395
                     /locus_tag="ACHBBW_001606"
     CDS             15153..15395
                     /locus_tag="ACHBBW_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9153287.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15483..15629)
                     /locus_tag="ACHBBW_001607"
                     /pseudo
     CDS             complement(15483..15629)
                     /locus_tag="ACHBBW_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15686..16000
                     /locus_tag="ACHBBW_001608"
                     /pseudo
     CDS             15686..16000
                     /locus_tag="ACHBBW_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16029..17288
                     /locus_tag="ACHBBW_001609"
     CDS             16029..17288
                     /locus_tag="ACHBBW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153288.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17341..18237
                     /locus_tag="ACHBBW_001610"
     CDS             17341..18237
                     /locus_tag="ACHBBW_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153289.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18248..19117
                     /locus_tag="ACHBBW_001611"
     CDS             18248..19117
                     /locus_tag="ACHBBW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153290.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19127..19802
                     /locus_tag="ACHBBW_001612"
                     /pseudo
     CDS             19127..19802
                     /locus_tag="ACHBBW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20008..21672
                     /gene="alsS"
                     /locus_tag="ACHBBW_001613"
     CDS             20008..21672
                     /gene="alsS"
                     /locus_tag="ACHBBW_001613"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9153291.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            21685..22395
                     /gene="budA"
                     /locus_tag="ACHBBW_001614"
     CDS             21685..22395
                     /gene="budA"
                     /locus_tag="ACHBBW_001614"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9153292.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            22525..23022
                     /locus_tag="ACHBBW_001615"
     CDS             22525..23022
                     /locus_tag="ACHBBW_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9153293.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         7579 a         3983 c         4812 g         6654 t
ORIGIN      
        1 aaaaaaaaag tttttccccc taattttttt agatagaaaa ttgtatagta tgatgagaga
       61 aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat
      121 ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct gttgtacaag
      181 gtgcggagat attaggcctg atccaaggaa gcggattagt ggtttctagg aattttccag
      241 ctttgactgt tttctattta gtgataagtg taggtctctt attaccaaaa tctctgatca
      301 atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca tctatgtttt
      361 ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga ttgaaggatt
      421 cagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta gtaattgcct
      481 catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa agtgaacgat
      541 ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaagca tatgagctgc
      601 gtaaaatgca tgtttctcat atgctttttt tattattatg taaagaatgc gaaaaacgtt
      661 tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg gatttacagt
      721 cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa agtattgttt
      781 actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg aaatacagat
      841 ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta
      901 ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt acaagaaaat
      961 ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa
     1021 gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga cggagttgat
     1081 gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcaccaat cgtcaataaa
     1141 gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac aggaaaaatc
     1201 actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac
     1261 ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac ccctgtgcag
     1321 tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac accaggagca
     1381 atcagctatt tagctttctc atatctcgat gattccacac aagcactaag tatcgacggt
     1441 gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc atatgaacat
     1501 atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta catgatgaca
     1561 gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac aatgatggaa
     1621 gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc cacggtagtc
     1681 gttactatta aggaaaaatc aactatgttt ccccacatat acatttttcc tttaattgtg
     1741 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     1801 caccgtttat tcgaaaaaaa gagctacgac atagaactgt cgtagctttt tttttgctat
     1861 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
     1921 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
     1981 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
     2041 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     2101 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     2161 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     2221 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     2281 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     2341 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     2401 tgtaatagat gacattgtta ttttagagac tggacatcaa attccagcag atgcagaggt
     2461 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     2521 caaagaaata ggcgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     2581 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     2641 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     2701 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     2761 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     2821 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     2881 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     2941 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaag tgatcgtctc
     3001 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     3061 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     3121 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     3181 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     3241 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     3301 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     3361 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     3421 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     3481 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     3541 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     3601 aaagaaaatc ttcgttcaat tgctaaagcg tcgtaacaat acagtagcta tgacaggtga
     3661 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     3721 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     3781 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     3841 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     3901 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     3961 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     4021 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     4081 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     4141 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     4201 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     4261 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     4321 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     4381 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagttaaa aaatttctat cgatatttaa
     4441 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     4501 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     4561 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     4621 taccatgaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     4681 aatgccggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     4741 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     4801 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt
     4861 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     4921 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     4981 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     5041 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     5101 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     5161 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     5221 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     5281 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     5341 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     5401 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     5461 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     5521 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta tacgcccgat
     5581 acatcttact catttgttcg ctcaacgcat tgatcgtctg atacaattct tcccattgat
     5641 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     5701 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     5761 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     5821 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     5881 tgactggtaa tgcgacatat aaaagatccc cattcaaagt agtacttttg cgcaaagcgc
     5941 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     6001 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     6061 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     6121 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     6181 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     6241 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     6301 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     6361 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     6421 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     6481 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     6541 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     6601 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     6661 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     6721 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     6781 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     6841 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     6901 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     6961 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     7021 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     7081 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     7141 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     7201 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     7261 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     7321 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     7381 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     7441 cccttcatgc ggaacccatt cgatttatga cgggagtttt tccggaataa aaaaaaatgg
     7501 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     7561 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     7621 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     7681 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     7741 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaacttcatc
     7801 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     7861 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     7921 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     7981 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     8041 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     8101 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     8161 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     8221 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     8281 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     8341 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     8401 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     8461 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     8521 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     8581 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     8641 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     8701 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     8761 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     8821 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     8881 tgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     8941 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     9001 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     9061 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     9121 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     9181 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     9241 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     9301 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     9361 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     9421 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     9481 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     9541 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     9601 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     9661 cgatattaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     9721 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     9781 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     9841 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     9901 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     9961 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
    10021 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
    10081 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
    10141 cactccaaca ggtaagcaaa ccacttatga agctttttat gaaacaatcg atacgaccat
    10201 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
    10261 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
    10321 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
    10381 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
    10441 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
    10501 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
    10561 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
    10621 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
    10681 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
    10741 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
    10801 gcacattcaa aaagacaagt gattgtcgta gatggtggtt tgcgccaaag ccgccgatcc
    10861 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
    10921 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
    10981 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
    11041 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
    11101 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
    11161 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
    11221 tcctccttca aactatctat aacaatctta caaaaaataa gtaactggag caaataaaaa
    11281 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
    11341 aaaatcttct atttcctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
    11401 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
    11461 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
    11521 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
    11581 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
    11641 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
    11701 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    11761 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    11821 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    11881 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    11941 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    12001 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt tattaattga
    12061 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    12121 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    12181 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    12241 agtccagaaa aaagtagtac caaaacggct accaccactg aacaaacaat ggctacctca
    12301 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    12361 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    12421 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    12481 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    12541 acaatccaag ttttgcagcc ctttgagatc gacacaaatg gtcagcaagt ggacttaggt
    12601 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    12661 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    12721 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    12781 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    12841 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    12901 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    12961 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    13021 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    13081 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    13141 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    13201 gttaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    13261 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    13321 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    13381 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    13441 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    13501 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    13561 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    13621 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    13681 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    13741 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    13801 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    13861 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    13921 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    13981 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    14041 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    14101 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    14161 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    14221 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    14281 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    14341 cagcagatga agccaagtga ctttttctaa aatattcttt acagtaggtt gataatatgt
    14401 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct atgagaaaag
    14461 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    14521 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    14581 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    14641 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    14701 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    14761 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    14821 gtatcaaaga aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc
    14881 aagggttctt ttttcttagc atgtaatttg aacaccctga tccagttttt ttctttaatt
    14941 attggtaggg gagaataatg gattagaata aatctaccta taagtagtag taaaaaaaag
    15001 agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc agaatattct
    15061 aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat acttatacgg
    15121 agcaagaaaa aatgaaagtt tgagacacat tcatgaatga tggaaaagta aaacaagtac
    15181 caagttcaac aaaaaagaaa aacatcctat tgaaagaagt cttgaaacgc tttgaccatg
    15241 gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt tctagtggcg
    15301 attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga agttcagacg
    15361 gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata ctcattgaaa
    15421 aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt tgtcatctgt
    15481 atctataata caaagccaag aacgttattc aaaatatgga tagctatgat aaaagctaat
    15541 gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa
    15601 aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta cgaataaata
    15661 ccaaaacgta cctcctaaaa ttagattcaa agaagatagc aaagaggaaa aagatagttc
    15721 tgcagcagct atagcggcat gtggattgct agaactttcc aaacaactgc ctgtttcaga
    15781 tcctcgtcat ttcgattacg aagagatagc aatcaaaatc ttggaagaat tacagaaaaa
    15841 ctatacaacg aaacgagtta acggatcaaa cggtctgctt ctccatgccg tttatgataa
    15901 aaacagctta aaaggtgtag atgaatgtgt gatctggggc gattactttt atgtagaagg
    15961 aatcactcgt ctagctaaaa catggtactg ttactggtga tgtataagag aagaggagga
    16021 ttcatataat gaagcgatgg aaaaaaatga tgattggatc aatagtttta ggaatgtcac
    16081 tcgtatttgg tgcttgtggt aatcaagaag acagtagtga agaatcagca aatgaaatta
    16141 ccgtttcgac atggaattat gaaacaacac ctgaattcaa agcgctcttt gaagcattcg
    16201 aaaagaaaac gggaatcaaa gtgaaagcag tagatattgc ttcagatgat tatgatacga
    16261 aactcaccac gatgctttct tctggagaca cgacagatgt attgacgatg aaaaatcttt
    16321 tgtcgtattc aaattatgct ttacgagatc agttagtcga tcaaacagaa cgaattagag
    16381 aattagacac tgatgctgct gaagaaacat atgaaatgta tgatatcgat ggaaaaacat
    16441 atgcattgcc atatcgaacg gacttttggg tactatatta caataagaaa atgtttgatg
    16501 atgcaggaat cgaatatcca aaaaatctaa cttgggacga atatgaagat ttagcaaaaa
    16561 aacttagtaa aaatgacgga caagtttacg gagcatatca acatatctgg cgttcgacaa
    16621 tccaagctat tgcagctgct caaaacgagg cgaatcttgt tgaaccagat tatcaatata
    16681 tgactgatta ttatgatcga gcattgagga tgcaaaaaga aggcgcgcag atggattttg
    16741 gcacggctaa atcaacgaaa gtaacctatc agtctcaatt tgaagaacaa aaagcagcaa
    16801 tgatgtacat gggaacttgg tatatggctg gcatactcgc aaacaaagaa gcaagtaaga
    16861 cagaagtaga atggggaatt acagctattc ctcagaagaa aaaaggagaa tcggtcacga
    16921 cgtttggctc accaacagca tttgctgtca ataaaaactc aaagaaacaa aaagcggcgc
    16981 aagaatttat tgaatttgca gccagtgaag aaggagcaaa agtattagca ggagtaggag
    17041 tagttccttc atatcgtacg gatgagattg atcagcttta ttttaatctc gctggaatgc
    17101 caacggatga aatttctaaa aaagcttttt ctcctgatga aatcaaactt gaatttccga
    17161 ttgatacgta tgggcctgca atcgataaga ttttacaaga agaatatgac ttagttttgg
    17221 ttggagatga aacgccggaa aaaggaacgg taaatatgga gaaacgtgta gctgcggaaa
    17281 aagaataatc atgaaaggag agaagccaag agataaaaca ctcttggttt cgtcatcgaa
    17341 atggagaata gtataaataa gaaagctaca aatcatttgc gtagaaaacg aacgtggatc
    17401 gcttattcgt ttatcgcccc gaatttcatt ggttttttcg tttttacatt aataccaatc
    17461 ctgttttcgc ttgtactagc atttatgaaa tgggattctt tttcaacgcc agaatttgct
    17521 ggactagata actttcgtaa aatggccaac gatgagacat tttggatttc tttgaaaaat
    17581 acttttttat ataccgctgc tgtagtgcct ggcacacttg tttgttcgtt agggctagct
    17641 ttactactaa atcagaaaat tagagggatg aagctttttc gtacgatatt tttctttccg
    17701 tatgttactt ccttagtagc aattgctgtt gtttggaata tgttgtttca tccaacagtg
    17761 ggacctatta atatcttttt aagacgattt attgaacatc ctccaggttg gacttcaagt
    17821 tcaaattggg cgctttgggc aattgtaatt gtcagtattt ggcgagggat gggttattac
    17881 atgattctat accttgcagg actacaaagt atccaaaaag aactatatga agcgtcttcg
    17941 ctagatggag caaaccgttg gaaacaattt ttgaatgtga cattaccttc gttgagacca
    18001 acaaccttct ttgtaacgat catgcttgtc atcaattgtt tcaaggtgtt tgaccttgtg
    18061 caagtgatga cggatggcgg ccctggtcga gcgacaaatg tgctggttta ccaaatctac
    18121 aatgaagcat tcaaaaaatt tgattttggt tatgcttcag ctatttcact tgttttgttc
    18181 gtaattgttt tgggcgttac ggttgttcaa ttttcttgga ataagcgtcg tacttaggag
    18241 gtgtgatatg gaagagagtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa
    18301 catgcccttt attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat
    18361 cattcctttt ttatggatgg tttctgcttc attcaaagca aataatgaag tgttaacgat
    18421 tccgattcaa tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg
    18481 tattccttta gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat
    18541 tcagttgttt acttctagtt ttgctgttta cgggttttct gaactaaatt ttaaagggcg
    18601 cgatatttta tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat
    18661 cccgcagttc attatgatga gaaaagtggg tttaacagat acgttgtggt cgttggtgct
    18721 attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag caatattaca gtagtatccc
    18781 agattcttta tgtgagagtg cgcggatcga tggcttatca gagtgggaaa tctatcgcaa
    18841 gatcatttta cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa
    18901 tacatggaac gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat
    18961 ccagttaggg ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc
    19021 tgcctcagtt gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt
    19081 tgtagaaggg atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg
    19141 ttttgacaaa aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag
    19201 cctttgtttt tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga
    19261 tggaaggaat gtcttttcct ttattttttc tttacttttt attggtccag gttgcatcgt
    19321 gatgataggt atgacagaag aattagcaaa agaaaaaagt ttttcaccag tacgtttgtt
    19381 cataaagacg tttcgccaat tttggctaaa gggatttttt tattggctat ttgcttggat
    19441 agttagtgtg ataatgattt ttgattgttt cttttttata cgtttttctt atggaaaatg
    19501 gttgattccg ttgtttgttt tacttgcttg ttttacttgc ttgtttatca gtttccttta
    19561 gcatcaactg ctggtatttt caggtgagaa atccagcgtc gaaaccaaat caagttctac
    19621 ggatagcgtt ctactatacg ttgaaaaaat ggtatgtgag tttactagat tttttactat
    19681 tgacaagttt gtttctcttc tttttcgtaa agccccaatg gtgtattcta ctaggtcctt
    19741 ctatcgtatt tggtttgatt tacttcaaca atcgtaaatt gatgagaact atggatctgt
    19801 agactttata aattgaaact ttcaattaag aatgacagtt ttttatgtaa aaactgtcat
    19861 tctttttcat tttttgaaac atatggctaa acaaatgaaa taaaacatgc tatacttttg
    19921 aatgaaacag gtttagaaac tgtatcactt atttgtgatt atttctaact aataattaaa
    19981 aataaagcat aaaaaggacg atgttcaatg gctgaaaaaa tgaaacaagg ttcgaaatct
    20041 gtagtagaaa gtttgattaa tcatcatgtt gattatgtat tcggtatacc aggtgcaaaa
    20101 atcgatggtg tatttaatga attagaggat caaggtcctg aattaatcgt gactcgtcac
    20161 gaacaaaatg ctgcatttat ggcacaagca atcggacgta ttacaggaga gccaggagta
    20221 gttatcgcaa caagtggtcc tggtgctagt aacttagcta caggtttggt aacagcgaca
    20281 gcagaaggtg acccagtttt agcaattgcc ggacaagtaa aacgaagcga tctattgaag
    20341 ttgacccatc aaagcatgga taatgcagca ctgttccagc caatcacaaa atacagtgca
    20401 gaaatccaag atccagaaac aatttcagaa gtgattgcca atgcttatcg tatggctaag
    20461 agttcaaaaa aaggcgcgag ctttatcagt atcccgcaag atgtagtgga tgcacctgtc
    20521 caaggtaatg tcatcaagcc attgagcgat cctaaattag gttctgcttc tgcagatgat
    20581 atccgttacc tagctgaacg cattagagaa gcaaaactac cggtactatt agtaggtatg
    20641 cgaggatcaa gtgaaaaaga aacgttagcc atcaggcaat tagttgaaaa gacagcattg
    20701 cctgttgtag agacattcca agcagctgga gtgatctcac gtgaattaga agcgcatttc
    20761 tttggtcgtg tgggtttatt tagaaaccaa ccaggagata tgcttttgaa acgaagtgat
    20821 ctagttattg caattggcta tgatccaatc gaatatgaag caagaaactg gaatgcggaa
    20881 aaagatgcgc ggatcattgt gatcgatgaa gcgccagctg aaatcgatcc ttttatgcag
    20941 cctgaaagag aattgatcgg tgatatctca gccactcttg atttattgac aggttcatta
    21001 gagcctcaac aagtgtcaga agatgcaaaa gaatatttag catctcttca agcaaaatta
    21061 acggagcggg acattgttca atcaaaagga gaagcaggta tcttgcatcc tttagaagtt
    21121 attaacacgc ttcagtcaaa agtaacagat gatatgactg ttaccgtaga tgtaggtagt
    21181 cattatattt ggatggcacg ccactttaga agttatgaac ctcgtcattt gttattcagt
    21241 aatggtatgc aaacgcttgg tgttgcattg ccatgggcga tttctgctgc attggtgcgt
    21301 ccaaatacac agatcgtatc agtttccggc gatgggggat ttttattctc tgcccaggat
    21361 ttagaaacgg cggtacgtaa aaaattgaat atcgttcatt tgatttggaa tgatggacac
    21421 tacaatatgg tagagttcca agaaaaaatg aaatatcagc gggcgtcagg tgtagacttt
    21481 ggtccagttg attttgtgaa atatgcagaa gcttttggcg ctaaaggaat ccgagctaca
    21541 agtgtagagg aattagaaaa agctttagaa gaaggatttg cgacagaagg acccgtaatt
    21601 attgatattc cgattgatta ccgtgataat gagaaattgg gagaaacaat cttaccagat
    21661 caattctatt aaggaagtgt cgacatgact gaaaaaatct tataccagca cgggacatta
    21721 ggagctttga tggccggctt gatggatgga acagaaacga ttgctcatat attagaaaaa
    21781 ggaactctgg gattgggaac attgcatggg cttgatggtg aagtcatctt tttagatggc
    21841 gtagcttatc aaggaagatc agatggctca gttgcccaat tagatggaag tgaacttacc
    21901 ccctatgcag caattacaga ttttacgcca gatactagtt tttctgtctc aaaaactgca
    21961 gatggagaac aattgaaaaa agacatatta gtaagagaag ccggggaaaa cctatttttg
    22021 gcagtgaaga taactggatt attcaaaaat atgcacatcc gtatcatgcc gaagcaagaa
    22081 aaaccttaca gaagactagc aaaaatttca gagtctcaac cagaattcca acaaagcaat
    22141 gtccaaggaa cattagtcgg gttctttacg cctgaattat tccaaggagt tgctgcagcg
    22201 ggatttcacc tgcattttat cgatgataca catacatttg gcggacatgt aatggatttt
    22261 gaagtggctg aaggaaaagt tgagattagc cgcatctcgt cccttgtaca gcattttcca
    22321 gttgatgatc cgacttttgt tcacgaaacg attgattatg ctgatttagc agcagaaatc
    22381 caacaagcag aatagaaaaa gacagcgaga tgcgttcaag gtgattaacc tgacgtatct
    22441 cgtttttttg aggagaaaac ctagcgttta caaaaaaact gagctataat cttctccata
    22501 gatcaaaaag gagcggtttt tatcatgtgg acagaaaaac agaaaaaaga tgcatacgaa
    22561 attttattat tacagcaaca aggattatta gaagcagaag ataactggtt agcaaattta
    22621 gcaaattcat ctgcattgtt gaatgagact ttgcctgaga ctgtctttgc aggatattat
    22681 ttattcgatg gtaatgaatt gattttagga ccgttccaag ggcgagtttc ttgcacacgt
    22741 atcaagatgg gaaaaggagt ttgtggagaa tcagctgaaa aacaagaaac attgatcgtt
    22801 gatgatgtca aaacccatgc aaattacatt tcctgcgatt cagccgcaca atcagaaatc
    22861 gtagtgccaa tggtaaaaga aggtaattta atcggtgttt tagatatcga ttgcggagta
    22921 acgaaagggt atgatgaaat cgatcaagca tttctagaaa aatatgtaga tcttttatta
    22981 gcaaatttta atataaatgc tttttcattt attaataact aaaatcgt
//
LOCUS       DAYYKC010000045        22686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000045 DAYYKC010000000
VERSION     DAYYKC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.045"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHBBW_001616"
     CDS             127..3654
                     /locus_tag="ACHBBW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9153294.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHBBW_001617"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHBBW_001617"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9153295.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHBBW_001618"
     CDS             7442..7783
                     /locus_tag="ACHBBW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9153296.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHBBW_001619"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHBBW_001619"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9153297.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHBBW_001620"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHBBW_001620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9153298.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHBBW_001621"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHBBW_001621"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9153299.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHBBW_001622"
     CDS             12994..13731
                     /locus_tag="ACHBBW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9153300.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHBBW_001623"
     CDS             13745..14560
                     /locus_tag="ACHBBW_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9153301.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHBBW_001624"
     CDS             14573..15220
                     /locus_tag="ACHBBW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9153302.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHBBW_001625"
     CDS             15236..15892
                     /locus_tag="ACHBBW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9153303.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHBBW_001626"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHBBW_001626"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9153304.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHBBW_001627"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHBBW_001627"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9153305.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHBBW_001628"
     CDS             18234..18752
                     /locus_tag="ACHBBW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9153306.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHBBW_001629"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHBBW_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9153307.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACHBBW_001630"
     CDS             complement(19639..20559)
                     /locus_tag="ACHBBW_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9153308.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACHBBW_001631"
     CDS             20685..21737
                     /locus_tag="ACHBBW_001631"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9153309.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHBBW_001632"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHBBW_001632"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9153310.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7561 a         3954 c         4827 g         6344 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttctta
//
LOCUS       DAYYKC010000046        22633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000046 DAYYKC010000000
VERSION     DAYYKC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.046"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHBBW_001633"
     CDS             complement(227..1114)
                     /locus_tag="ACHBBW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153311.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHBBW_001634"
     CDS             complement(1111..1980)
                     /locus_tag="ACHBBW_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153312.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHBBW_001635"
     CDS             complement(2117..3403)
                     /locus_tag="ACHBBW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153313.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHBBW_001636"
     CDS             3676..4254
                     /locus_tag="ACHBBW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9153314.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHBBW_001637"
     CDS             4254..5183
                     /locus_tag="ACHBBW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153315.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHBBW_001638"
     CDS             5180..6916
                     /locus_tag="ACHBBW_001638"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9153316.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHBBW_001639"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHBBW_001639"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9153317.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHBBW_001640"
     CDS             complement(7812..8321)
                     /locus_tag="ACHBBW_001640"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9153318.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHBBW_001641"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHBBW_001641"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9153319.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHBBW_001642"
     CDS             complement(9622..10713)
                     /locus_tag="ACHBBW_001642"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9153320.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHBBW_001643"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHBBW_001643"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9153321.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHBBW_001644"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHBBW_001644"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9153322.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHBBW_001645"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHBBW_001645"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9153323.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHBBW_001646"
     CDS             complement(14676..15650)
                     /locus_tag="ACHBBW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9153324.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHBBW_001647"
     CDS             complement(15631..17514)
                     /locus_tag="ACHBBW_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9153325.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHBBW_001648"
     CDS             complement(17532..19757)
                     /locus_tag="ACHBBW_001648"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9153326.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHBBW_001649"
     CDS             19872..20906
                     /locus_tag="ACHBBW_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153327.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHBBW_001650"
     CDS             complement(20973..21986)
                     /locus_tag="ACHBBW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153328.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHBBW_001651"
     CDS             complement(22064..22252)
                     /locus_tag="ACHBBW_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9153329.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7228 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aac
//
LOCUS       DAYYKC010000047        22441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000047 DAYYKC010000000
VERSION     DAYYKC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.047"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..651)
                     /locus_tag="ACHBBW_001652"
     CDS             complement(199..651)
                     /locus_tag="ACHBBW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9153330.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(664..1074)
                     /locus_tag="ACHBBW_001653"
     CDS             complement(664..1074)
                     /locus_tag="ACHBBW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153331.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1349..1561
                     /locus_tag="ACHBBW_001654"
     CDS             1349..1561
                     /locus_tag="ACHBBW_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM9153332.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1564..1776
                     /locus_tag="ACHBBW_001655"
     CDS             1564..1776
                     /locus_tag="ACHBBW_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153333.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1916..2071
                     /locus_tag="ACHBBW_001656"
     CDS             1916..2071
                     /locus_tag="ACHBBW_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153334.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2068..2292)
                     /locus_tag="ACHBBW_001657"
     CDS             complement(2068..2292)
                     /locus_tag="ACHBBW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9153335.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2370..2558
                     /locus_tag="ACHBBW_001658"
     CDS             2370..2558
                     /locus_tag="ACHBBW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153336.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2697..2825
                     /locus_tag="ACHBBW_001659"
     CDS             2697..2825
                     /locus_tag="ACHBBW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153337.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2884..3219
                     /locus_tag="ACHBBW_001660"
     CDS             2884..3219
                     /locus_tag="ACHBBW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153338.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3452..4393
                     /locus_tag="ACHBBW_001661"
     CDS             3452..4393
                     /locus_tag="ACHBBW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM9153339.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4395..5285
                     /locus_tag="ACHBBW_001662"
     CDS             4395..5285
                     /locus_tag="ACHBBW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9153340.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5328..6128
                     /locus_tag="ACHBBW_001663"
     CDS             5328..6128
                     /locus_tag="ACHBBW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9153341.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6143..6994
                     /locus_tag="ACHBBW_001664"
     CDS             6143..6994
                     /locus_tag="ACHBBW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9153342.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7033..7194
                     /locus_tag="ACHBBW_001665"
     CDS             7033..7194
                     /locus_tag="ACHBBW_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153343.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7195..7656
                     /locus_tag="ACHBBW_001666"
     CDS             7195..7656
                     /locus_tag="ACHBBW_001666"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9153344.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7682..8134
                     /locus_tag="ACHBBW_001667"
     CDS             7682..8134
                     /locus_tag="ACHBBW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153345.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8240..8434
                     /locus_tag="ACHBBW_001668"
     CDS             8240..8434
                     /locus_tag="ACHBBW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153346.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8465..8851
                     /locus_tag="ACHBBW_001669"
     CDS             8465..8851
                     /locus_tag="ACHBBW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153347.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8848..9051
                     /locus_tag="ACHBBW_001670"
     CDS             8848..9051
                     /locus_tag="ACHBBW_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9153348.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9048..9344
                     /locus_tag="ACHBBW_001671"
     CDS             9048..9344
                     /locus_tag="ACHBBW_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153349.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9420..9836
                     /locus_tag="ACHBBW_001672"
     CDS             9420..9836
                     /locus_tag="ACHBBW_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9153350.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10097..10234
                     /locus_tag="ACHBBW_001673"
     CDS             10097..10234
                     /locus_tag="ACHBBW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153351.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10644..10874
                     /locus_tag="ACHBBW_001674"
     CDS             10644..10874
                     /locus_tag="ACHBBW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153352.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11033..11866
                     /gene="terS"
                     /locus_tag="ACHBBW_001675"
     CDS             11033..11866
                     /gene="terS"
                     /locus_tag="ACHBBW_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM9153353.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11859..13274
                     /locus_tag="ACHBBW_001676"
     CDS             11859..13274
                     /locus_tag="ACHBBW_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM9153354.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13286..14815
                     /locus_tag="ACHBBW_001677"
     CDS             13286..14815
                     /locus_tag="ACHBBW_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9153355.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14733..15785
                     /locus_tag="ACHBBW_001678"
     CDS             14733..15785
                     /locus_tag="ACHBBW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM9153356.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15787..16104
                     /locus_tag="ACHBBW_001679"
     CDS             15787..16104
                     /locus_tag="ACHBBW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153357.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16231..16854
                     /locus_tag="ACHBBW_001680"
     CDS             16231..16854
                     /locus_tag="ACHBBW_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM9153358.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16869..17759
                     /locus_tag="ACHBBW_001681"
     CDS             16869..17759
                     /locus_tag="ACHBBW_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM9153359.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17782..17997
                     /locus_tag="ACHBBW_001682"
     CDS             17782..17997
                     /locus_tag="ACHBBW_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153360.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18009..18350
                     /locus_tag="ACHBBW_001683"
     CDS             18009..18350
                     /locus_tag="ACHBBW_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153361.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18350..18721
                     /locus_tag="ACHBBW_001684"
     CDS             18350..18721
                     /locus_tag="ACHBBW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153362.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18714..19109
                     /locus_tag="ACHBBW_001685"
     CDS             18714..19109
                     /locus_tag="ACHBBW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM9153363.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19111..19488
                     /locus_tag="ACHBBW_001686"
     CDS             19111..19488
                     /locus_tag="ACHBBW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM9153364.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19489..20097
                     /locus_tag="ACHBBW_001687"
     CDS             19489..20097
                     /locus_tag="ACHBBW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9153365.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20097..20438
                     /locus_tag="ACHBBW_001688"
     CDS             20097..20438
                     /locus_tag="ACHBBW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153366.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20680..22441
                     /locus_tag="ACHBBW_001689"
                     /pseudo
     CDS             20680..22441
                     /locus_tag="ACHBBW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8114 a         3280 c         4635 g         6412 t
ORIGIN      
        1 ttcatcattt ttttgttcta attcataagc ttcttttttt attgtgttag ctttcgacag
       61 tttagtgatt cctataatta ataaaataag tccaatcaat aataacattc aaaactcctc
      121 atttctttga tataattatt gtagagttta ctcagaaatg aggaaagagt ccatgtgaaa
      181 gcatgggctt ttttattttt aatgttgtaa tactaaatgt tcaagattat gaggaagccc
      241 taaatattca ataatttgga actgactcat atcagataaa tcttgttcat ctattgttct
      301 agttaacatt tcaattgcaa acatattagc ttcagcttct ataccataaa tcatacaact
      361 aaggttgttt gctctaaaag ctgctgtgca aataccttta tggagtctaa cgtgggctat
      421 ttcatgccat aatacaaatc ttttcataga aggtgatagg ttttgattaa ttaatatcac
      481 ataacatctt ttattgtgta tcgtttgtcc taatgtttta ttccctaaat cagtttcaat
      541 taaatctata tcagaatatt tgattatttt atatggatta atcgcatcag atccaaactc
      601 tacaaataat ttttctattt cttctaatat ccagtatttg tttaaatgca taccaaagcc
      661 cacctattga cgatattttt taggagtgaa ttttttcttt gctttaactt tggttaaggc
      721 aagtgcaagt tgaatggcat cgtataattt ttccttatct tcatcgtcta aaggttcacc
      781 ataataattt gtttcagcat ctgaattcaa gccttcaagt aatttttcag cttgtaaagc
      841 aatatcttgt ttatcttttt ctgttaaatc ataataatgg cgtttatcag tacgtcctaa
      901 aagataatca gttgatacat taaagtaatc tgcaactttt tgtattttat ctccagatgg
      961 agtactttta ttccatctag acatgcttcc atttccgaaa tttaactttc tttcaagttc
     1021 tgccaacgtc attgaacgtt cttttaataa gatttttata cggtctaata gcataggata
     1081 tgcattcctt tcaaaatacc gacaaaaaaa gtatttttct gcaaattatc tatttacaaa
     1141 tagagaattt gcagttatac tagttttgta agcaagatat ttacggcata caaagactaa
     1201 aagcaataaa ctaatagtct ccccagacat cagtaagctc gctttttgtg cgctgtattt
     1261 tgtatgctta cataatagca tatttgcagt ttttgtcaaa cttattagac aaaaatatta
     1321 gaaaatattc gataaggggg tatgaagcat gactttaaaa cagcaagtat taattgcttt
     1381 agtaaagaaa ggttggagtc aaagagagtt agctcgacga atgggtatat caattactta
     1441 tttacgagat atcttcatcg aaaaaagaaa acctaaggaa cgtttaaaac aaatcgaaga
     1501 acttttggat atcaagttag aagtagattc taatgatcag ccatcagaac aggaggcgta
     1561 aaaatggtac aacgtttgag caaagcggag tttaaagaga aatataagta ttctgaaagt
     1621 acttatcaaa gaagaatgaa ggaatttaaa aaatcacgtt ttagtgaggg gtataacgca
     1681 gttacttcca aagaaataac aattgatgtc gatttatatg aacagtttag aacatggcgt
     1741 tctaaaaatc gatttagagt tagagctgtt tgttaatagc taaaaaatct aaattcacca
     1801 cttctgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac gttttttcat
     1861 tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg atgacatgaa
     1921 ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa ttattgaaac
     1981 agaagaaggg aaaaaagtag cagaagttac attgaccgat gcaactcctg ctgacggtta
     2041 tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat cgtttccgtg
     2101 actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag atccttgatt
     2161 tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat gtgcagtcgc
     2221 tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta caacatgttg
     2281 attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa tagaaatgga
     2341 gaaattcaat aaacgaaagg agaagtgata tggcatatac cttgcaacaa gaacatcaaa
     2401 ttctcggttt gattaaacaa cgcaggaaac aattacaaga tgaccgtgca gcgcttagaa
     2461 aagccgatga gctatcagat aggcaagctg aactaattgc ttctgaactt gaggatttga
     2521 gaatgctaga aataaaaaat agggagatta gattatgaag aagacagaca cactttttat
     2581 aggattcatt ttggggttat tagtgattgt agcgcaccaa agtattatcg ggggaagctt
     2641 gttcgcagca ttgatggttt taatcaatct gcttgattca aaagaaagga gcaactatgg
     2701 cacgagaaga agcgctaaaa atcggtaaag tgattgctga taatcggtgg ggaaatagcc
     2761 gccctattat tttaagcaag caacatatcg aaaagcaaaa agcatggcaa caaataaaaa
     2821 agtgactctg ccagccagca aagagtcaca tacaaattta taaaactagg agaattatat
     2881 cacatgaata agaaaattga aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa
     2941 ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt
     3001 gagacaacag aaattttact ttgtcttgct atgcaagaag aacatttaga tgaaaatctc
     3061 ccattgtcag cacatattat gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat
     3121 gaagaagaaa atcaaccttc aaatcatact tttgttatta ataacaagga agatttagct
     3181 gatgtgatga ctcgtattct caagggggaa tttcaatgat gaatgaaaaa acaattgatc
     3241 tactcattat tttatttttc acgttagtga ttgtagcgaa aagttatgta atctttggct
     3301 tgttattcgc aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac
     3361 atgaacgagt tagcaaatct tgacaactat ttaactgatc ctgattatgc taaaccacca
     3421 tatgaagcac caattgatga ggaggatgaa gatgagtaaa tctaccttag aaatgagcca
     3481 tcaagaatgg cttgaagacc gtaagaaagg catcggaggt tcggatgttg gaacaatttt
     3541 aggattgaat aaatggaaat ctccttatca actatggcta gaaaaaacag gacaagtagt
     3601 acttgaagaa tcaggaagtg agcccgctta ttggggtaat gttttagaag aagtggttgc
     3661 taaagagttt caggaacgta caggcaaaaa agtacgcaga agaaaccaag tatttgaaca
     3721 tccattacat ccatttttaa gagcaaatat tgatcgggaa gtagtgggag aaaatgccat
     3781 tctggaatgc aaaacagcta atcaatttct cggtaaagaa tgggaaggcg aagaggtacc
     3841 gcttagctat ctctgtcaag ttcaacatta catgaacgtt ctaaacaaag actattgtta
     3901 catcgctgtc ttaatcggtg gtcaaaaatt tatctggaag cggattgaac gagatcaaga
     3961 gctgatcgat acaatcactg aacaattagt agagttttgg gaaacaaacg ttcttggagg
     4021 tatcgagcca attattgatg gtagtcaagc gacagctgat ttcttgaaag agaagtatgc
     4081 agatgttgaa gacgttcaaa cgacgttacc aattcatttc gatgaactag ttgaacagaa
     4141 aaacgaactc aaacggacta agaaagaaat tgaatcagct atccgtcaag tagacaacga
     4201 gattatcagc gagctaggta aacgtgaagc cagtatcggt atcactcaaa aaaacatcat
     4261 cagttggaaa tttgtccgta cgagacgtat gaactcgaag aaactagcag agaaatatcc
     4321 agatgtcgca aatgatgaag agatttataa cgttactgaa tcaagaaggc taaccgaaag
     4381 ggagatcaaa taatatggca acaaatgaat cgttaaaaaa tcaattggca gcaaagccac
     4441 agaaacaagt tgcaccaggt caattaggac ttaaagcttt gatgaataca ccaacaatga
     4501 gaaagaagtt cgaagaggtg cttcatgaca atgctaatgc ttttatgtcg aatgttatga
     4561 ctcttgtatc taatgacagt tatcttgcag atagtgaacc gatgtctatc atgagtggtg
     4621 cgttaactgc tgcaacatta aatcttgggc tagataagaa tttaggttat gcatatttag
     4681 ttccattcaa tagtaaaaac aagcaaacag gaaaatggga aaagaaagct caattcatgc
     4741 ttggctataa aggatatatc caattagccc aacgatcagg taaatacaaa gcattaaatg
     4801 tgatcgaagt ttacgaagga gaactaaaaa gctggaaccg actgacagaa gagtttgagt
     4861 ttgatccaaa tggtagaacg tctgatgaag tcattggata tgttggctat ttcgagttac
     4921 tgaatggatt caaaaaaact gtctattgga ccaaacaaga aatcgaagct catcggattg
     4981 ctaacaataa agatcgcgat aagacaaagt taagtggtgt gtgggcatct gattacaatg
     5041 caatggcacg aaaaactgtt ttgagaaatc ttctttctaa atggggaatc ttgtccatcg
     5101 aaatgcaaga agccaccaca tcggatgaga gagtccaaag ggttcaagaa gacggcagca
     5161 ttattgctga aacagaagtt gaagaagata ttcctgaaag aaaagaagca gaggttatat
     5221 ctgaagaaaa tgaagatgta caaactggat tatttgatgc atctaatccg ccgttaaaca
     5281 aataatgagg gagctttctc ccttgctttt ctagaggaga aatacgaatg aacacaggat
     5341 atgtcaaatt acatcgaaaa gtgatgaatt cattcgtttg gaccaatcct tatatgtata
     5401 aattatggaa cttgtgtttg atgaaagcga gtcatgagaa tcgcaagata ctttttaatg
     5461 gaaaagagat acaagtgaac agcggagaat tcgtcacagg gcgcgatgct atcacatctg
     5521 agatgaacga aggcgtgaaa cgtgaacatc aagtgaacag cggttctgta tggagatggc
     5581 taaaacaatt tgaaaaaaac ggaatgttga acatcaaatc aactacgaaa tacagcgtta
     5641 tatcaataaa aaactggtct ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca
     5701 caacaagtga acaacaagtg cacacaaaca agaatgaaaa gaattataag aatgaaaaga
     5761 ataataaaac atcatcgtcg caaccacgca aaaagcgtgt ttacgacacc gactcagttt
     5821 actacattct cgcggaggag ttattcaaac agatttgtca gaatcaggaa atcaaaaagc
     5881 cgaatctgca aagttgggca gataacattc ggaaaatgat cgagatcgac aagcggaccg
     5941 aaaaccaggt acggggaatg attgaatgga gtcagcacaa tgtgttttgg gcatcgaata
     6001 ttttatctgc taaaaaattg cgagaaaaat acgacacaat ggcagcgcaa gccaatcgtg
     6061 attataaaac aaaacaaact aaaacgcttg aatacgagaa atttagcaca gatgagttgc
     6121 ctatttgaga ggaggcgtag gcatggaaac tgttggggaa atcatggaaa agctgataca
     6181 gaaagtgctt gtccaacgtg gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg
     6241 tacgaagaac cctgatggct cagaacgttg taagccaacc tgtatgcaat gtgggtataa
     6301 agcattgcga gttcaggaag acttacaaac agaacgaatt tacaacgaga gcctgaaagc
     6361 aagagcaatc aattttttca aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg
     6421 cacattgcag aattatcaaa ttgtcgatca agaaacaaga caagcggttg aagtaaccaa
     6481 acgctttgtt aattcagtct tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca
     6541 aggaacaggt aaaagtcacc tagcgatggc agcggcttgg gaagtcttga agcgatcaaa
     6601 ctacgataaa aaaatcttat ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc
     6661 gtacaacaat cctgaactca gaaaaacgat tgagggatcg ttgattgcag atatcaaaac
     6721 agcggattta gtcatcattg atgatatcgg ttcagaacta ggaaaagatg catcagatag
     6781 tcgagcgttt ggcataaaca cgctaaattc gttcttggat gcacgacaga acctagcaac
     6841 gattatcaca acgaacttgc ttggtgaaga actgaaaaaa gcttacggta cgagaacaat
     6901 atcaagaatg tttgtcaact ctgatggatt tacgatggta ttttctcaaa cagcagacaa
     6961 gcgcataaaa ccagtcaaag gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc
     7021 tgtcgtcaga taatgttgca aattataggt gatcgtgaca tggacgaatt atctcaacaa
     7081 gagagtcgaa tggttcggaa gataagtaag ctaattattt atcgggatac agaaagattt
     7141 ctcactgttt taggtaacgt ctacgagaat ccagagttat tggaggtaga gtaattgcca
     7201 aaaatattag atgcatgttg cggtagtaga ttgttctggt ttgataagaa caattctgac
     7261 gtaacattta tggattgtcg tcagcagtat gaagaattat caacaggtca tgtgatcaat
     7321 gttgatccag atgtggtcgc agactttcgc gatatgcctt ttgaagatga tgtgttcgat
     7381 atggttgtat ttgacccgcc acacttaaga catgtcggcg aaaactcatg gcttgccaaa
     7441 aaatatggaa aattagacga actttggcca gaagacatca ggcaaggttt tgcagagtgt
     7501 atgcgagttt tacgaccttc aggaacatta atttttaagt ggaacgagga acagatacca
     7561 ctttctgatg tgttagaggc tattggagaa cagccattgt ttggtaataa acgaagcaaa
     7621 acacattggc ttgtgtttat gaaaaaattc agctaacgga ggaaacaaag gaggaaaagt
     7681 aatgaagatt gtattaaata aatgttttgg tggctttggg ttgagccatg aagcaaaaat
     7741 ggaaattttt aaaagaaaaa atattgaagt attcccatac atcaataact ttagctatga
     7801 ctcagatgat gaatacacga gatacacagg ccaaaagcta ggttcgatgg attttatcta
     7861 ctatttcaaa aaagacccga aaatcgacaa agttacagga atttatagcg aaattgaccg
     7921 actgtacgga attgctgatg attccagttt ttcaagtgat tcaaacagag gtgataagga
     7981 cctcgttgct gttgtcgaaa aactaggaag tgaggccagt ggaccatatg caagtcttaa
     8041 agtcgttgat atacccgatg gggctgagtg ggaaataagt gattatgacg gcgttgaaac
     8101 tgcacattat ggttttcaaa caggtagtat ttagtccact aatgacaact aaagaggagg
     8161 cagaaggatg agtgaagatg aatactttga acagtagtct attaacgaca gagattttta
     8221 actggaaagg ggtgtagtca tggacgcatc ggaagtagta aataaaatta atagtctcaa
     8281 agaggttttc ggagacgtcg aagtagtgat aaacgcacaa ggctatgaca acggattttt
     8341 taagcagatt accgacatta gcattcagca aggaatggaa gacgaagatg gaaactttat
     8401 cgatgaagta gccattttag tgacttgcga gtaagtaatt gacagctaaa gaggaggcag
     8461 aaggatgaaa aaattgcacc aactcatatc tgagaaagaa agcgaactcc aaaatttaga
     8521 agatagtttg ggattggggt ttccaattgt tgagcaagct aaaatgactc aaatcagtca
     8581 tctacgactt gaattggaag atttaagaca aatagaaaaa tcaattcaac tcaacgacaa
     8641 ccagaaaatc gtgttggagt ggttgaaaag cgagacaatt ttaactagag aagcaccaat
     8701 attatctgtt aatgcttttt ctgataaaaa tttactagga aaattacctg ataaagtacg
     8761 caaagcttat aaactattgg cttgtaaaca agaatatgaa gtcctatcag cattcgctca
     8821 atggggatta gaacaggagg aagcggaatg aacaacagac atcgcagaat aacaaaacta
     8881 agaaaacagg aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca
     8941 gaagaaacat ataaagtggc aagtcagttt gttgctgatg cgagtaatgc tattcgtaag
     9001 tttgggattt cgatattaaa tgatgatcgt aaatgggagg aaatgagatg aaaataaaag
     9061 acggatttta cgctagtagt catggtatcg gcggtttaat gctagacatg ccgataaaga
     9121 accctaaaac acgtcagaaa tcaaaattca aaatcggtga catggtccga tgtaaagcag
     9181 aagagttcat ctatccgttt cgtggatatg tagagcatgt ctataatcat tcagcaatca
     9241 ttcacattga aaacacgatg gaatgcgata agtggttagc gaaaagcaaa gataatttag
     9301 cagtggctcg tttggtggat atggaactaa tcaatgacaa ataaaaaagc cggatcgctc
     9361 cgactgattc aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga
     9421 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
     9481 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
     9541 ttattacgga tatgccgaag gcaccgaagc acggcaataa ggcagaagac gcgatcattc
     9601 agatgatgga tatagaagcg gagagagacg caatactagc ggctttgatg gctcttagtc
     9661 tgattagccg tcagatactc tactacagct tctgtgacgt aaacaagcac tctaattatg
     9721 aaatagggca attgatacga ggatacggag agaagaatgt agagaagttg aaatccatcg
     9781 cattaatcga atttgcagaa gcatacaaaa aaggcgtgtt agttcagtat cgttgatttt
     9841 gtagggtttt tgtagggata gtgtagggtt tttgagcggt ttaacgtgat attatggtag
     9901 tgtcgaaaga ttagtgatag gtctgagaca aaataaaatg taagggagga aatctccctc
     9961 atcgttttaa cttaagcttc gatagacagc aacggaaata ttaagaataa ggatgtgaat
    10021 ttcaactcct tctaaattgt tcttattatc tatcatccgt tgttgtctat taatttatgc
    10081 atcggaggaa aaacgaatgg ataaagaaat caaagtaact gtcaaattag atttgactga
    10141 actaaaagaa ctgctcaaca aggctagtga ccaagtcgaa cagttacaag aaactttaga
    10201 tgaaattgct aattttaaaa tccaagtttc ttagcagtat attttttagc agctgcactc
    10261 ataaaatcat accaagtatc agaaaaatat aacttaacta aataattaaa aaaggtgggt
    10321 aaatagaaat gacggcgtta ataatttcaa tttttgcgct ctgtcttaat gtctatatga
    10381 ttggatttaa aaatggacaa aataaaaaat agtagcagcc gagaataatt ttatagtgtc
    10441 actgtggcgg aaatagaaga cgcaaaggtg gaggtaggaa gcgtatggcg cactatcggg
    10501 accttgtaca aaactagaaa ggactagtac gtgtgtggtg cgagccctag ggaggaacag
    10561 gataaccgcc tgtgtgtagg ttgctattac atattagatc actctttgag tggtcttttt
    10621 atttacataa aggaggctac ataatgagaa actactggta tatatcgcta actaatgaat
    10681 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtccaa atcaaaaaga
    10741 agtactccat tgttgaaatg accagagaag ctacgccaaa agagattgat aaatgcaaac
    10801 ttctttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga
    10861 atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt
    10921 agacaaagtt tccgatttaa agatattgat tgaagctgat ttaatgctaa aagatattga
    10981 aaattagaaa acaaaactca acctaagaag attgcgaggt ggtgtgtatt gaatggcaag
    11041 acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa
    11101 gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa
    11161 atcgcaagac aaatggagcg ctgaattgaa aagtaacgtt accaatggca aaagtaacgt
    11221 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaacag
    11281 cagagcatca ccgccaaaga gaaataagaa tgctcttaaa actggtgagt atgaaacaat
    11341 attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc
    11401 ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa
    11461 aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
    11521 aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
    11581 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
    11641 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
    11701 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
    11761 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
    11821 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
    11881 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
    11941 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
    12001 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
    12061 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
    12121 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
    12181 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
    12241 ggtttgctac ggctagaaca gcgactaaac cagaaaatat gcaaggtttt cacgaggacc
    12301 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
    12361 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaaccct aataatattg
    12421 aaggtgtctt ttacgattcc cacaattcag accgtgataa atacagagtt cataaagtat
    12481 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
    12541 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttcccaaa ggtgcgttgg
    12601 attcatttat cagtcttgaa acggttgaat tggctacaga aaaacaaatt agtgattctt
    12661 tagtcaataa aacaacggtt gctcatattg gtgttgacgt agctcgatat ggtgatgatt
    12721 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
    12781 gtagcactat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
    12841 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
    12901 ccgaccgcct agaagaactt atagaagaca agcattatcc ttttgaggtg tttggagtga
    12961 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
    13021 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
    13081 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
    13141 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
    13201 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
    13261 ttattcaatt ttaggaggtg agtttttgat tagtacagta ttatctttag agagatataa
    13321 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
    13381 tatagaatat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
    13441 cgaaaagaat gaaatagaca atagaccaaa gcctaacact gatattgtaa aagttaataa
    13501 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
    13561 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
    13621 aaaagataaa ttcttaaaaa atgatataga acttccaaca gttccagaag cttttaacaa
    13681 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
    13741 tcaaggagct tgtggagtag ctttccgatt gttagagcct gtggaagaag atgatggttg
    13801 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
    13861 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
    13921 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
    13981 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
    14041 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
    14101 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
    14161 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
    14221 tatcaactta aaagacccta ctgcaaaagc tgaatatgtc acaaaaaaat tagcaaaaga
    14281 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
    14341 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
    14401 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
    14461 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
    14521 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
    14581 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
    14641 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
    14701 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
    14761 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
    14821 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
    14881 acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa
    14941 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
    15001 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
    15061 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
    15121 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
    15181 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
    15241 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
    15301 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
    15361 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
    15421 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
    15481 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
    15541 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
    15601 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
    15661 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
    15721 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
    15781 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
    15841 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
    15901 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
    15961 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
    16021 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
    16081 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
    16141 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
    16201 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
    16261 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
    16321 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
    16381 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
    16441 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
    16501 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
    16561 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
    16621 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
    16681 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
    16741 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
    16801 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
    16861 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
    16921 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
    16981 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
    17041 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
    17101 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
    17161 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
    17221 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
    17281 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
    17341 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
    17401 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
    17461 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
    17521 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
    17581 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
    17641 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
    17701 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
    17761 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
    17821 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
    17881 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
    17941 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
    18001 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
    18061 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
    18121 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
    18181 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
    18241 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
    18301 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
    18361 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
    18421 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
    18481 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
    18541 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
    18601 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
    18661 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
    18721 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
    18781 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
    18841 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
    18901 ctagaaaact atcaagcggt cgaattgttg gagcagttat aaacaatgaa cattatttta
    18961 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
    19021 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
    19081 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
    19141 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
    19201 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
    19261 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa acctaataga
    19321 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
    19381 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
    19441 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
    19501 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
    19561 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
    19621 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
    19681 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
    19741 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
    19801 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
    19861 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
    19921 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
    19981 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
    20041 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
    20101 caaaatatca aacaacgatt aaattaacga aaaaagatga agaaggtaaa tatgagcaag
    20161 tacaatttaa atctgctgaa tttctaccag gcccagtcgt agaagaagca gcagaagtaa
    20221 tggagggaat gcaaaatgct gtagatggaa aatcagtatc agaagcatta agtcgagctt
    20281 attcatttat tgcagatatt ttgtttgaag gacagtttac tggtgaagat tatcgtcaag
    20341 ggatcgatgc tcgagaaatt gcttcgttga caggtaagtt attaaaatct gttactgcag
    20401 gctttgatga aacttataca gaaacgaaaa aaaagtaagc gaagctctca aatatccttt
    20461 ttttaaacat tcgattactt atcgagaatt agatttaaag acgcaattac tcgaagcagg
    20521 ttggacgtta ccagaaattg aacgtactga ctttgacgag ttaatgcgtc tttttgcttt
    20581 tcgagatgca gtaaaagagc atgaggatgt tgagtactac gataacttca cccaatttta
    20641 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
    20701 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
    20761 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
    20821 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
    20881 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
    20941 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
    21001 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
    21061 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
    21121 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
    21181 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    21241 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    21301 ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    21361 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    21421 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    21481 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    21541 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    21601 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    21661 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    21721 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    21781 tcattaaaga ttatgggggc aatagctgct gctaattcat ttgttttaat tattggtgca
    21841 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac
    21901 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    21961 aaggactggg gaattgatac ttggaattct gctaaataaa tggcatcaaa tgcagttgag
    22021 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    22081 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    22141 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    22201 aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt
    22261 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    22321 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    22381 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    22441 a
//
LOCUS       DAYYKC010000048        22400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000048 DAYYKC010000000
VERSION     DAYYKC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.048"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1804
                     /locus_tag="ACHBBW_001690"
     CDS             119..1804
                     /locus_tag="ACHBBW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9153367.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1818..3101
                     /locus_tag="ACHBBW_001691"
     CDS             1818..3101
                     /locus_tag="ACHBBW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153368.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3171..4040
                     /locus_tag="ACHBBW_001692"
     CDS             3171..4040
                     /locus_tag="ACHBBW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153369.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4037..4873
                     /locus_tag="ACHBBW_001693"
     CDS             4037..4873
                     /locus_tag="ACHBBW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153370.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4899..5966
                     /locus_tag="ACHBBW_001694"
     CDS             4899..5966
                     /locus_tag="ACHBBW_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9153371.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5963..6790
                     /locus_tag="ACHBBW_001695"
     CDS             5963..6790
                     /locus_tag="ACHBBW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9153372.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6835..7860
                     /locus_tag="ACHBBW_001696"
     CDS             6835..7860
                     /locus_tag="ACHBBW_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9153373.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7857..10040
                     /locus_tag="ACHBBW_001697"
     CDS             7857..10040
                     /locus_tag="ACHBBW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9153374.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10040..11170
                     /locus_tag="ACHBBW_001698"
     CDS             10040..11170
                     /locus_tag="ACHBBW_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9153375.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11167..11844
                     /gene="pgmB"
                     /locus_tag="ACHBBW_001699"
     CDS             11167..11844
                     /gene="pgmB"
                     /locus_tag="ACHBBW_001699"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9153376.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11928..12455
                     /gene="msrA"
                     /locus_tag="ACHBBW_001700"
     CDS             11928..12455
                     /gene="msrA"
                     /locus_tag="ACHBBW_001700"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9153377.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12656..13342)
                     /locus_tag="ACHBBW_001701"
     CDS             complement(12656..13342)
                     /locus_tag="ACHBBW_001701"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9153378.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13422..14960
                     /locus_tag="ACHBBW_001702"
     CDS             13422..14960
                     /locus_tag="ACHBBW_001702"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9153379.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15080..15547
                     /locus_tag="ACHBBW_001703"
     CDS             15080..15547
                     /locus_tag="ACHBBW_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9153380.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16081..16509
                     /gene="rplM"
                     /locus_tag="ACHBBW_001704"
     CDS             16081..16509
                     /gene="rplM"
                     /locus_tag="ACHBBW_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9153381.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16525..16917
                     /gene="rpsI"
                     /locus_tag="ACHBBW_001705"
     CDS             16525..16917
                     /gene="rpsI"
                     /locus_tag="ACHBBW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9153382.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17005..18231)
                     /locus_tag="ACHBBW_001706"
     CDS             complement(17005..18231)
                     /locus_tag="ACHBBW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153383.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(18303..18605)
                     /locus_tag="ACHBBW_001707"
     CDS             complement(18303..18605)
                     /locus_tag="ACHBBW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153384.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            19153..19872
                     /locus_tag="ACHBBW_001708"
     CDS             19153..19872
                     /locus_tag="ACHBBW_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9153385.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19874..20716
                     /locus_tag="ACHBBW_001709"
     CDS             19874..20716
                     /locus_tag="ACHBBW_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9153386.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            20713..21663
                     /locus_tag="ACHBBW_001710"
     CDS             20713..21663
                     /locus_tag="ACHBBW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9153387.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            21678..21911
                     /locus_tag="ACHBBW_001711"
     CDS             21678..21911
                     /locus_tag="ACHBBW_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9153388.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            22242..>22400
                     /locus_tag="ACHBBW_001712"
     CDS             22242..>22400
                     /locus_tag="ACHBBW_001712"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153389.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINA"
BASE COUNT         7383 a         3861 c         4621 g         6535 t
ORIGIN      
        1 ttcttatttg tatttttgtg tatatcaaaa ttatttaaaa aaatgctatt gacagtaagc
       61 gttttccata atatgatcaa atcgttggga acgctcccaa aaaagaaagg ggaatccgat
      121 gtatacaaaa cttttacaag cgatttatcg cgactcagat ttgtctgaga aaaccgcctc
      181 tatgattgaa caaaaagtca gtgtttatca gcagaagaaa ggccaggcag gcaaactgca
      241 ttgggacgaa gaagatattt tcttgattac ttatggggat caattttttg aagagcagca
      301 accaactttg cgtacattta agaaatttta tcaaaagtat ttggcagata catttagagt
      361 tgtacatttc ttgccatttt tcccatatac atctgatgat ggtttctccg ttgtaaatta
      421 tcaggcaatc aacgaccgtt tgggcacatg ggaagatata gaagaaatga gaagcgaaac
      481 acggttaatg ttcgactttg tctgcaatca tatgtctgcc gaaagtactt ggtttcagga
      541 atatttaaac ttgaatgata cgtacaaaga tttttttatt gatttgccac tggatacaga
      601 tacaagcagc gtgactagac caaggaccag cccgttactt acaacgttca ttgacaaaaa
      661 cgggaaagaa aaacacgtat ggacgacttt tagcgaagat caagtagact tgaattttaa
      721 aaatccagaa gttttattga aaatgatcga tattcttttg ttttacttag aaaaaggcgc
      781 ttcatttatt cgcttggacg ctgtgggatt tttatggaaa gaactaggaa cttcttgtat
      841 acacctgcca gaaacgcata aaatcatcca attattccgt gcaatcgctg atgaagcagc
      901 tccaggaacg gtattgatca ctgagacaaa tgtgccacac aaagataata tcagttattt
      961 tgggaatggc gagaatgaag cacagatggt ctatcagttt tcgctaccac ctttgaccct
     1021 tcaagcgatt cgttcgggta atacaagtaa gttgaaagaa tggttaaaaa ccatcgattt
     1081 cgcatcgaaa aaaacaactt tttttaattt tttagcctcc catgacggta tcggattgaa
     1141 tccggtacga ggtattctgc cggaaaaaac gatccttgaa ttagtggaac agctgaagaa
     1201 agagggtacc cgagtaaact acaagaaaaa cccagatgga acgaccagtc catatgagat
     1261 aaacgctacg tatctagatg cgttaagttc tccaacagat acgacggaat ggagaagcaa
     1321 acgcttttta gctgctcatt catttttggt aacgttacca ggcgtgccgg caatttatat
     1381 tcaaagtgtg ctgggaagtc gaaatgatta caaaggcgta gaaaaaacag gagagaatcg
     1441 aaaaatcaat cggcaaaaat ttttgttagc agatattgag aaagagttgc agacagatcc
     1501 attaagaaca gcagtatatg gtgaattgac ccagatgcta tctattcgaa aaaaagaaaa
     1561 agccttccat ccgaatagtt tgatttactt actggactca gcagattctt ttatcagttt
     1621 tattcgcgaa gcgaaggaag agaaagtctt agttatccat aatttttcca atcaatcagc
     1681 aggttatctt ttaccaaaag gagattacct agacctgttt tctggacaaa ttatccattg
     1741 cagtcaagaa cgaaagttga tattccagcc gtatgaatat atgtggctga aaataataaa
     1801 ataagaggtg gaaaaaaatg aaaaaaatta ttgttactac ctgtctgcta gctactggat
     1861 ttcttcttac tgcttgtggt gaaagtcaaa gctctaacga atcacaacag acaacgattc
     1921 aattcatgca ctcttctgtc gaacaggaac gattaagtgt gatcaatgaa ctggttgctg
     1981 attttgaaaa agaaaatcca gacatcaaaa tcaagcaagt gccggtagaa gaatcagcgt
     2041 tcaatacaaa aatcgttacg ttagctcgtt ccggtaaatt accagaagta atggaagtca
     2101 gtcaggattt tgctaaagtg atggataaag atgaactgat tgatcagacg gcagtccaaa
     2161 acgttatcga ggaagctgga gaagataatt attatgacgg agccaaaaac cttgttcgtt
     2221 cagaagatgg aaaaagctat attgctgcac caattagcgg ctgggttcaa ggtatttggt
     2281 atgacaaaga aaaattatca gaagctggtt tttcagaacc agaaaattgg gatgatattt
     2341 taaaaattgc tcaacatttc acagataaag agaataaaca atatggaatt gctatgccta
     2401 ctgctgaagg aacaatgagt gagcaagcgt tctctcaatt tgctttgtca aatggagcaa
     2461 atgttttgga tgatgaaggc gaggtaacga tcgatacgga aaagatgaga gaagcattgt
     2521 cgttttatca aaacttatcc caatacacga tgccaggctc taatgatgtg acagaaatca
     2581 aagatgcttt catgaatgga acagcaccaa tggcaatcta ttcaacttac attttgcctt
     2641 ctgtttatga agcggggaac tcagaaaata tcggttttgc tatcccaaca caaaaagatc
     2701 aagctgtcta tggcacagta tctggattga cgatttcttc tggtctagac gagaaacaaa
     2761 aagaggctgc tgaagcattc acagctttct tatctcaacc gaaaaacatg gaaaagtggg
     2821 tcttgatgtc tcctggtggt gctcaaccag tgaataaaca agtagtcgaa ctggatggtt
     2881 acaaagaaaa tgaagtcatc aagtcttttg gcgagctgcc atctgagatt gctagtgctt
     2941 ttgatgaagt acaagtcttt ggactagtcg gggaaaagaa tttcacgaaa atgggagata
     3001 tcacaagctc aggtgtgatc ggaaaagcag tgaaccaagt aactgtcgga aacgaagatg
     3061 tcgatcaagc tttagctgac gcacaaaaaa atacgaaata acaagaagtt ctgccaatgg
     3121 tgttcgaaaa aagtagatag ctattggcag attatcaggg ggaggtttca atgaatcgtc
     3181 caaggaaaca ttctgactta aaattagcca tgggactttt agcaccaagc atgcttttat
     3241 tagctgtttt agtgctgatt ccaatgcttt ccaatattca gatcagtttt ttcgatatgc
     3301 cgctgaatcc taactcagac gccgttttta tcggattaag caattatctt gaaattttaa
     3361 gtgatagtga attttatcgt tctttaggtt taacagttgc ttacacattc ttagttgtag
     3421 caggcagtac agctttagga ttagccgtag ctattttgct taaccgcaag ttcagattta
     3481 ggaaaacggt gcgttcactg attttattaa gttatgttac gccatctatt tctttgattt
     3541 ttgcttggaa atatatgttc aataatagtt atggaatcgt caatgatctg ttagtagata
     3601 ggttgcagct tttcaacgaa gcaccgctgt ggttcgacca gccagttgct agcttcttta
     3661 tggtcgtttt gtttgctatc tggagatact atccgtatgc gtttatttca tttttggcta
     3721 ttttgcaaac agtcgacaaa acgctttatg aagcagctga aatggacggt gcaaatgctt
     3781 ggcagaagtt caagatcgtg acactgccgg caatcaaacc agtactagca actgtgattg
     3841 ttttacgatc tatctggatg ttctatatgt ttacagatgt ctatctactg acgaataaag
     3901 tcaatatctt aggcgtttat ttgtatcaga cagcctttgc gttcaatgat cttggaaaag
     3961 cagcagctat atcgattcta ttgttcatca ttattgcagc ggtcgtcttg ctctcacgaa
     4021 agaaggtgga cttgaaatga aaaataaaaa gagcaggaaa atcttatttt acgcgacgat
     4081 tttgttgcta ttagctgtca atttgtttcc atttttgatc atgctgatga catcattcaa
     4141 ggattcacaa gaagcaattg caacgacaca aactattttt ccgaaagaat ggacttttga
     4201 acattatcag gatattttca atgctgagat ttttccattc atcatgtatt tcaaaaatag
     4261 tttagtgatc tccttgatag cctccgtaac tgcagtagtg attgggatca gcggggcata
     4321 cgctttgtca aaactacgct tcaaagctcg ctcgaaaatc aatgctagct tttacaccgt
     4381 ttatatgttt tccggtattt tgatcgttgt tccattattt aaaatcattt ccggtctagg
     4441 tttgtatgat accaatacag cactgattat cacgatggtc gttcaaacac ttcctactgc
     4501 gatattcatg ctaaaaagtt attttgacac gattccgact gatattgaag aagcagcaat
     4561 gatcgatggt cttagccgga tccagatcat tattcggatc attataccat tggctatttc
     4621 tggaatcatt tctgtcttcg tttattgctt tatggtttcg tggaatgatt atttatttgc
     4681 ttcgattttc ctgtcgacct ccgaaaaatt tactttacca atcggtttga acacattatt
     4741 cagtacacca gactacattt ggggacgaat gatggccgct tcattggtga ccgctttacc
     4801 agtcatcatt atgtatgcgt tatctgaacg atttattaaa ggtgatttag tcgaaggcgg
     4861 cgtaaaagga taaaaagaaa agaaagagga aagaaaagat gaaaaaatta gttgcattag
     4921 aaccaagaat agccggattc aaagattacg aagaccgtga gatcgaaaag aacgaagtac
     4981 gtatccgagt aattttcgct actccaaaac caggaacaga aattgtcgac tttagaggat
     5041 taagtccatt tatcgatgaa gaattttcag aagaatggcg cctattcttg ccaagagaag
     5101 aaggaagcca acgcggtgtg gtatttggcg aatttcaatt aggaaatatg attgtcggta
     5161 cgatcgaaga aacgggcagt ttggtgacag agtatcaaaa aggagagtta gtctgcactt
     5221 atggacccat tcgtgaaacg ctgattacaa acggtgttga taattataaa ctacgtaaac
     5281 ttgatcggtt ggatcaatgg aaaaatgcag tatgttacga tcctgctcaa ttcgctttag
     5341 gtggtatccg ggaagcacat gttcgagcag gtgatgctgt agcaatcttc ggtttaggcg
     5401 ctatcgggca aattgcgatt caattagcta aaaaagcagg tgctactacc atcgttggga
     5461 tcgatcccat agcgcagcgt agagaattag cagaaaaata cggtgccgat tacacgctag
     5521 atcctatgaa agcagatgtc ggttttcgtc taaaggaatt gacagataaa cgtggagtag
     5581 attcagttat tgaaacgagt ggcagtgctg ccgcattaca agcttcttta agagggatcg
     5641 gctatggcgg gatcatttct tatgttgcct ttgctaaacc ttttccagct gggtttaatc
     5701 ttggtcgcga agcacatttc aataatgcaa aaatcgtctt tgctcgagca tctagtgaac
     5761 caaacccaga ttacccaaga tggaaccgta aacgaatcga ggacacatgt tgggaattat
     5821 tgatgaacgg ttacttggat tgtgaagaaa tcattgatcc agttgtgcca tttgcagaaa
     5881 gcgcagaagc ttacatgcgt tatgtagacc agcacccaga agaaagtatc aaaatgggcg
     5941 ttcaagtccg aggaggagaa caatgaaatt cggtacgcaa gatcaagcat ttttcccaag
     6001 agacatttta gaaaaattta gatttatcaa agactgtgga tttgaagaat acgaaatcga
     6061 cggtaagttg ttaatagaaa atatcgacac agtcaaacag gcaatcaaag aaacagggat
     6121 cccagtagtg acagcttgta acggctacga tgggtggatc ggtgacttta tcgaagaacg
     6181 gcgtcttaat ggtgtacgtc aaattgctga gattttgcgt gcactaagcg aggttggagg
     6241 caaaggaatc gttatcccag cagcttgggg aatgttcact tatcgattac cgccgatggt
     6301 ttcaccccga agcaaagaag gagactataa agcagtcagc gaatcacttc gttatttaga
     6361 taaagtagcc gaagaaacag gtacggttat ttatttggag cctttgaacc gataccaaga
     6421 ccatatgatc aatttgttag cggatgcgcg tcattacatt gactctaatc aattgaaaca
     6481 tgtgcagatc atcgctgatt tttatcatat gaatattgaa gaagatgata tttcaaaagc
     6541 attgactacg caaaaagagt atgtgggtca tatccattta gcagataatc atcgctacca
     6601 accaggatct ggcgcaattg attttactcg ccattttcaa acgctacttc aaaatgagta
     6661 tgaaggttat atggtatatg aatgtcgttt gcgaggagaa aatgaagtga aaatttatca
     6721 ggaatcattg caatacttga aaaactgtct ggaaacagcg cgtcatttaa atgatttaga
     6781 gaaagaatag agaaagaata acaataagta aataagcaaa ggagggagaa ataaatgaag
     6841 aaagtagcaa tcataggcgc tggacaagta gcagaaaaag tacatgctgc ttattatcaa
     6901 aaaagatcag atgtcttaga attggccgct gtggttgatc cagtcagcca gcgagcagaa
     6961 gacttttgcc gacgtacagg attttctcat gcgtatacat cagttacaca aatgctgaat
     7021 gagatttctc ccgatatcgt gagtgtctgt acacccaatc gctttcactt cgaacatgta
     7081 ttggctgccc tcgaagctaa tgcttcagtg atgtgcgaga aaccaccagc tatgacagct
     7141 gatcaagcaa gaaagatggc agagacagcc gataaaaacg ggcagatttt ggcttatgga
     7201 ttccagcatc gtttttctga cgaagcaaca atgatcaaga atcatcttga tgttctcggg
     7261 gatatttatt acgtagaagc gaatgcgctt cgtcggtctg gtgttccagg ctggggaacg
     7321 tttatcgaca aagaacttca aggaggcggg cctttgatcg attatggcat tcacatgctt
     7381 gatacagcga tgtatattac tggatttcct aaagtaaaaa gcgttcgggc gtacagcttc
     7441 caaaaaattg gtacgaaaaa aaattcaggg aaattcggga aatgggatcc aagcaaattc
     7501 acagtagaag atgcaatttt tggcacactg gaattagaaa acggcaagat catccaactg
     7561 aatacttcgt ttgctttgaa tatcagagaa gacaaactga atgtggcttt ttgtggcgat
     7621 caggcaggtg ctaatctgta tccaggcgaa ctattcgaag atgtagatgg aaagctggtt
     7681 tccttacaaa agaccacgcc agttaatgac ccacatgaac taagtatggc tcattttgtg
     7741 aatcgggcgc tcggtgacca gactcagatg attgcagatg gctggcaagg atatgtcatc
     7801 cagaatctgg tggaaaattt atatcgttcg gcagaaacag gagaagttat taccttatga
     7861 aagtagaaga aaaacagttt gatttagagt atatcaataa gtatgcgagt cagatgacca
     7921 ttggaaatgg attttttggt atcagaggaa gccaggagga agactatcgg aatcaagtaa
     7981 ggggaatgtt tgcagctggt gtatataacc gacctttagg agcagaatca gccgagttag
     8041 tgaatctccc agatgtcatc agatgggaaa taaaaatcga cggtgaacat ttttctcttg
     8101 agacgatgaa agttcttgca tacgagcggg cactagattt agaaatggga gagcttaaac
     8161 gctatgcgct cattgaaact gctcaaggaa aaaaagtgga agtcattact tgtcgtgttg
     8221 ctgatcaaaa taatctccat caggcagccc tgaaagtggt catcaagccg ttgaccgaag
     8281 actgccaagt agatgtgaaa acgggtatcg atggacagca aaccaatttt ggtacacagc
     8341 agttggttga aaaagaactg cgagtatttg gtgacgattt gatcagtgca tcctaccaga
     8401 caacagaaag taaaattgat attgggattg cagctaaatt caataaagca ggtgtattcc
     8461 aagcaaaaaa tcgccggatc accgcttcct tttcagaaaa gatcagaaaa aatacgtcct
     8521 ttacgctgga gaagatcatc gtgctctcta cctctcttca gcaaacagac ccgtttaaga
     8581 aaagtattgc ccaagcccag aaaagcgtta gttatacaga aattttagag cagtcgatgt
     8641 cttattggac agatttttgg catcagcatc ggataacgat ccaaggcgat tcttctttcg
     8701 atcagttagc gatggacttc gctgcttacc atttacagat tatgactccc cgcaatgatc
     8761 caagtgttag tgtaggggcg aagggactaa ctggcgaagg ttataaaggg catgttttct
     8821 gggatacgga aatttttatc ctgcctttct ttttatatca gtttcctgaa gtagcaaaga
     8881 atttgctgct gtaccgttat gatcgattag aaggcgcacg gcaaaaagca agagattatc
     8941 actatcaagg tgcacttttc ccatgggaaa gcgcacacac tggtcaggaa gagacaccca
     9001 aatttgctgc attgaatatc aaaaccggaa caagacaaga agttgcttca gctgccgcag
     9061 aacatcatat cagtgcagac cttgcttatg ccgttcaaga gtatgtccaa gcaacagagg
     9121 atcaatcgtt tttagaaaca aagggaaaaa cgctcattca tgaaacagca gctttttggc
     9181 tgagcagagc tgtaaaagtc gatgatcatt tagagataca cagagtcatt ggtccagatg
     9241 aatacacaga atatatcgac aataatgcat atacgaacta tctggcgcat tataatgtgg
     9301 ccttagctgt taaatatcaa gctggcgatc cgcttttttt gaaagaatgt caaaattttt
     9361 tagataagtt atatttgcca gtgcctaaca aagaacagct gattccgcaa gatgacacgt
     9421 atctcagtaa gccagttatt gatttgacca cttacaaaga acgtcctggc agccagtcta
     9481 ttttacttga ttatgcccgt agcgaagtaa atgaactcca agtcagcaag caagcagatc
     9541 ttgtcatgct gttatactta ttacctgagt atttttctca accaatcgtt tctaagaatt
     9601 tgcattacta tgaaaacaaa acaatccacg actcatcgtt gagtaaagcg attcacgcca
     9661 tagaggcttg ccgtatccaa gaaatggaat gggcgtatca tttattccaa gaagcgtgcc
     9721 tgattgattt aggaccagat ccgcattcat cagatgacgg aattcatgct gcttcattag
     9781 gagcactatg gctagcagtt atatttggtt tttcaggtat tacagtaacg aatggaactc
     9841 tttctatttc tccaagactt ccttcagcat gggaaaaaat ttcttttcct tttatttgga
     9901 aaaatcacag gttgctattt gttttgacaa aaaaacaaat agtaataaaa aaagagacga
     9961 aagaaacatt acctattttt gtcaatggag accaatacca gctggaaaaa gaactgatct
    10021 tggaacggag gggaaaataa tgcgtattct gattgcgcca gattctttca aagaaagtct
    10081 gacagcctta gaggcagcac aagccattca atcaggtttt caagaagttt ttgttcaggc
    10141 tgaatatgat ctgctaccta taggcgatgg aggagaagga acggtttcat cattagcaga
    10201 cagtttaagg ttgaagcggc aaacagcagt cgttactaat gcatttggca aaaacaaaga
    10261 aatccagtac gcttcagacg gtcaaactgc gatttttgaa atggcagaaa tcgtcggatt
    10321 gaaagatgtc ccactaaacc aacaacagcc acttaggtta tccacgcgag gagtaggaga
    10381 actgatacga caattgttgc aacaaggtgt tcgacacatc atcatcggcg ttggcggaag
    10441 tagtaccaac gatggcggca tcggcatggc agaaggtcta ggttatgctt tttacgatca
    10501 agataatcaa aaagtagaag cactgggaga aaatctaggt aaaattgcta cagttgataa
    10561 aacgaacgtg cctattgagc tgattaaagc caaaatcact gtcattactg atgttactaa
    10621 tcttttatgc ggaaaagaag gcgcttcttt tgttttcgga ccacaaaaag gcttgaaaga
    10681 aaatgaatta gcagtggctg accaagccat gcatgatttt tatatgagat ttgcccctca
    10741 agtaatcgag gcatcgggca gtagtgctgg tggtggaatg ggtgcaggac tgttagcatt
    10801 tgcttcaggg aaaatagtct ctggcatcga ctacgtattg gatttactgg agttcgataa
    10861 acgggtacag caagcagacc tcgtaatcgt aggcgaagga cgtctagatg gacaaagtct
    10921 aaaaggaaaa gcgccagttg ggattgctag aagagtacca gagggcgttc ctgtgatagc
    10981 tatttgcgga agtgttggta aaggaagcga aaaagcagca gatgttggca tttcggccgt
    11041 ttttccgatc cttcctgcag ctgtgccttt acaagaagct ttacagcaag ccactgataa
    11101 tcttcatcgc acagctaaaa acatcgcatc tttacttcaa gtcgttcatt ttcaaggagg
    11161 taatcgatga aaatcaaagc agcactattc gatttagatg gcgttcttgt agacacagct
    11221 cgttatcatt acgaagcgtg gctggtttta gctaaccagc tgtccattcc attcactgag
    11281 aaagaaaacg agcaactgaa aggaatatcc agaaccgagt cactagagcg attgctgtca
    11341 tttggaaaaa tggaacagaa attctcagaa aaagagaaat cagcctttgc tgagcaaaaa
    11401 aacaatctat atcttcaagc gattcaaaaa atggatgaaa catccgtttt gcctggtgca
    11461 atagctgttc ttgagtactt gaaaaaaaca aacatcaaga tcggattggg atcagcaagc
    11521 aaaaatgcac gcctcatttt agaaaaaacg aatttgacaa gttattttga tgtccttatt
    11581 gacggcacgc aagtatcaaa agcaaaacct gatccagaag tttttctcaa aggagcacag
    11641 caattgaatg taccgccaaa tgcatgcttg gtcattgaag attcagaagc tggttgtcaa
    11701 gcagctttgg ctggtaacat gcatgtattg ggaataggtg aaaacataaa tcttccttct
    11761 gctgaatatg tgattcctga tttaaccgtt tttgatcaag tacgttcgtt ttggcattta
    11821 tcagaagccg tatcacgtat ataatagttt ccgttttttc tgtcacaagc aaaacgttat
    11881 cctccttttg gaaatcaagg tatgatgaga aaaaaggggg attttatatg acaaaaaaag
    11941 cagttttcgc aggcggatgc ttttggtgta tggtcaaacc atttgaagag cagccaggga
    12001 tcatttctgt gacatccggt tatactggcg ggcatgtacc aaatccaact tatgaacaag
    12061 taacaacagg cacaacaggg catacagaag cagtagagat cgaatatgat ccagaagtta
    12121 tttcttatga tcagttggtg gaaatctatt ggcagcaaac cgatccgact gacgcattag
    12181 gacagttcgc tgatcgcggt gattcttatc gtccagtgat ctactataat gatgaagaag
    12241 aacgaaatat tgcagagcgt tcaaaacaag cattgcaaga cagcgggcga ttcgataagc
    12301 caatcgtgac aaaaatcgaa ccaagaagcg aattttatcc tgcagaagat taccatcagg
    12361 attattacaa gaaaaacaag ctccattaca atctttatcg agaaggttct ggacgtgcag
    12421 gtttccttcg tcgacattgg ggcgtcaaga aataagaagc cgaaaaagct acttatggaa
    12481 caccgtatat cagaggttgt aaaaccagca ttttgagcta atactgaggg tcagctgatt
    12541 gaacaccatt tagtggagag gttgtgaacc tgtcgcctag ctccaagtca caaggatttg
    12601 gtaagagaac actatatatc agaggttgtg acaagtcgtt tgtcacaacc ttttttcaat
    12661 acctaagatg aaggacaatc agcaacgcac tgctcaaaaa aggaatgaaa ggcagctttt
    12721 ccaacttttt tttatgacag ataaagtaaa cagaaaaaac ggatattcca aacagactag
    12781 caagaaacaa gatctgaagc aactcttcta gagacaagcc accactccaa gaaagcatca
    12841 acagagtatc gccagctccg agataggatt gaccatagcg aaggcacaca ccgcctacga
    12901 taaacgttaa cagcacactt tgccattgga agacgccggc ctgcaaccaa aaaatccata
    12961 aaattgccca agaacagaat agcgtgtgag aatggaccat gaagtaaaat aaatccatca
    13021 acgaaaatag caaagccgaa agcatccata tgaacgtcga aaaatttctt gcggaagaaa
    13081 aagtaaagaa ccaggcaaaa agaccaccac ataaggcttc tgccaacaaa tagtagaagg
    13141 gcaaccgaca cctgcaatag cggcagcgaa atcgttggac gatgatcgag agaatcggaa
    13201 tcaattcata ccaagaaagc gaacgacagc atttgacaca atgagatcta ggataaataa
    13261 tagagtgacc taacggaatc cgctgggcgg ttaaacataa aaatgatccg atacagcaac
    13321 caatgataaa ataaagtaac acgccatctc ccccttttca aaaccaagat acgcaagctt
    13381 aaactaaatt tttgtacata cataaaattt gaggtgaaaa aatgaacgtt tcaatcggat
    13441 tagatatcgg aaccacacaa accaaagcag ttgctttcac agatgaagcc caagaagcag
    13501 catccgctta ttttcgctac cctttgatcc aagaaacaca agggatggca gaacaagatc
    13561 aggaattgat ctttcaaggt gtctgcaccg ttataaaaga agtaattaaa cagttgcctt
    13621 cagacacaat cattcaattt gtctccttct cctctgcgat gcatggcctg atattgttgg
    13681 atgaagagaa aacaccgtta tctcgaatga tcacttgggc agataaccga gcagcggacg
    13741 aagcagaaaa actgaaatat accgtgcttg gcaaagaatt ttatcggcag actgggacgc
    13801 cgattcatcc aatgacacca ttgaacaaaa tcaagtggct gcaaaacagc caacctgaat
    13861 tattcaaaaa aactgcttat tttgtcggta tcaaagacta tatcttttat agattgtttg
    13921 gcgaactgat cagcgactac ggaacagctt caggaactgg atattttgat atccatgaat
    13981 tccaatgggc agaagaagtg cttgactact tgaacattcg aatagagcaa ttgccgcaag
    14041 tctttccttc cacctatcaa gtaacaggtc taccatcaaa aacagcagca gaactaggtt
    14101 tgccaaatga tatatcattt gttttgggag gggcagacgg gccgctttct aatctagggt
    14161 taggtgcttt tggtgatacg atcgccgctt tgaccgtagg aacgagcgga gcacttcgtt
    14221 ttattactac tcagcctcag ttccatccag aaatggagac gttttgttat gttctggatc
    14281 agaaacattg ggtagtggga ggagcgacta gcaatggtgc tggcatcttt gattgggcag
    14341 ggcatacatt tttacaggaa gtcgttcaat ctgctaaaga aaaaggagaa aatccatatg
    14401 atgcactgct ttcggctatc gcttttgtgc cagcgggtgc caatggtctg atttttcatc
    14461 cctatttact tggtgagcga gcaccgcttt gggacgcaga tgcgtttggc agttttatcg
    14521 gtttacagcg gcaacataca gaaaaagaaa tgatgaaggc gattctagaa gggatctgtc
    14581 tcaaccttta tcggatttta aaaggagttt gccaggaaaa tcagctcaca gagatccgtg
    14641 ctacaggcgg gtttgctcaa tctccgattt ttcgtcagat gatggctgat gtactgggtt
    14701 acccgcttgt tttccctact gtcacagaag catccgcatt aggagctgtg attttaggct
    14761 ggagaagcat cggaaagatc caaagtatca gtgaagcaca ggaattgata gaattgaaag
    14821 agcaagttgg agtacaagaa actgatcata aaaaatatca gaaaatctat ccattatttg
    14881 tttcgacaca aaagcattta gcacagactt atcagcaatt tgctcaactg cgagaagaat
    14941 tagctgattc tgaaaaatga ttggctttac ggataaaaaa gcgaactgaa aatcgttctc
    15001 aggtaaatga ttttgttgta cttttcattg aaagtctact attattttaa gtgtaagaaa
    15061 atgttaggag gcaagaaaga tgaaattcca acaaaccaaa gaagtattaa atcaattagt
    15121 tgcagatcta agccaaatgt ctgttgttgt tcaccaaaca cattggtaca tgagaggacc
    15181 aggtttcctt acattgcacc caatgatgga tgagttcatg gatgatttga acgctcagtt
    15241 agatgaagta tccgaacgct tgatcacatt agacggttca ccttattcta cattgcgtga
    15301 atttgcagat aacacaaaaa tccctgatga aattggccgc tgggatcgta ctattccaga
    15361 acgtttggaa acacttgttg caggctatcg ttatctagca gatctttacc aaaaaggaat
    15421 cgaagtttct ggagaagaag gcgacgatcc aacacaagat atctttatcg aattcaaaac
    15481 agccacagaa aaacgtatct ggatgatcca agctcatttg ggcaaagcgc cagagatcga
    15541 cgcgtaaaaa ctgttgttga acttgaccga aaaagaggtt gtgaaatcag cgttttgacc
    15601 tgagtattag agaaacaaac gtcaaaatag ttcctcatat tttgccgttt tttgagctaa
    15661 taccgagggt cagctgattg aacaccgttt accaagaggt tgtaagcctg ccgttcagct
    15721 ccgagtcaca agaaacaatt ttaaaaaaca gcttcttatg ttttatggat attgtgactt
    15781 gtgaccgagg agttggcagg agaacaccgt ttattcaaaa taaggggttg tgacaagact
    15841 tttgtcacag cctctttttg tttttcattt tgacaggaaa cgaccctttt atataaaaga
    15901 aagaaaagaa gtaaagtttc atataaattt ttcaatcaag cagaaaaacg ttgacattga
    15961 ctagattagc gggtaatatg ttaaatggta ttgtttgccc cacaaagagc cccggaaact
    16021 cgaagtgtat gtcaaacatc agaacttaaa ttggaggaaa ataacgtgcg tacaacatat
    16081 atggccaaac caggcgaagt agaacgtaaa tggtatgtag tagacgcaac tgatgttcca
    16141 ttgggacgtc tttcaacagt tgttgcatct gtactacgtg gtaaaaataa accaactttc
    16201 acacctcatt tggatactgg cgatttcgtc atcgtcatca atgcagacaa agtgaaatta
    16261 acaggtaaaa aagcgactga caaaatttat taccgtcaca gcaactaccc aggaggattg
    16321 aaatcaatca ctgctggcga attgcgtgct aaaaattctc gtcgtttgat cgaaacttct
    16381 gtaaaaggta tgcttcctaa aaacacttta ggccgcaaac aatttacaaa attgaacgta
    16441 tacggtggag cagaacatcc acatgcagca caacaaccag aagttttaga catcacaaac
    16501 ttaatctaat aggagggaat ttcattggca caagttcaat atatcggcac aggccgtcgt
    16561 aaaaacgcag ttgctcgcgt acgtctagta ccaggaactg gcaaaattac tgtaaataaa
    16621 aaagatgttg aagaatacat tccacatgct gacttgcgtg aagtaatcaa tcaacctttc
    16681 gctgttactg aaacaaaagg cgcatatgac gtatttgtta acgtaaacgg cggaggatac
    16741 gctggacaat caggagctat ccgtcatggt atcgctcgtg cattgctaga agttgatcct
    16801 gacttccgtt tagctcttaa acgtgctggc ttacttactc gtgacgcacg tatggtcgaa
    16861 cgtaaaaaac caggtcttaa gaaagcacgt aaagcttcac aattctcaaa acgttaatat
    16921 cctttatata tcaacgtttc tggcactttt caattatttg gaaagtgctt cttttgtttt
    16981 taagaacaca tttaagaaca catatcaatc acacttattc aatcctctct gaaaagcttc
    17041 aaaaacagcc ttatcaacta tatctggtgt gttcacatat atctttgttg tagaaatatc
    17101 tgagtgtgtt aaagcatttg aaatttgatt catatctgct ccaccttgtc tagctatagt
    17161 tgcataagta tgacggagtt tgtgtggaga taaatgaact aagtcaggat gccttctttt
    17221 aattgaattg attcgatagt ttaggtaatc ggcatgaaca ggacaattta cttctccaga
    17281 tggtttagta taagtaaaga gatactgatc taacgtttgc ttaattttta atttcaacaa
    17341 ttgatcagct tgtaccgatt tccattcact tagcaatgtc attacaactt ctggtacttg
    17401 aatgacggta tcttttcgac cttttgtaaa cttttttctt tgatatttat ctaaagtatg
    17461 tttaagacgg accgtttgtt tttcaaaatc aatatatttc cattgcaaag cgtaagtctc
    17521 actttttcta tcaccaagat acagggtaat cataaaaagt gtgtaatcat gaaaagtaag
    17581 taaatgatta ttaaaatcat catttactgc ttctatccaa ttagtaagtt cttttgatga
    17641 tagtgcttgc ttaccttctt gtattctttt ttcttctaaa gcattttttt taggtgcagt
    17701 gatactttga ataattttag tagttcgatt aaattcgata taatttagta tttctgcaat
    17761 atcgaacatg gaacgaacat atccttttat gatttttata tttgcatatt ctttcgattt
    17821 tttggttaat tcatctgata ttatctcagt attgatattt agataattca ttgcgtattc
    17881 accaaacata ggtaaaatat gcaaccgaaa tatatctttt gtattactaa tggttatatc
    17941 actaggaggc ctgtttgatc taattgtttg acctaattca tatctaggaa gccatttaga
    18001 ttcgtaaaat tttttaaacg ttatttttcc ttttaattct aatgagtttg cattctcctc
    18061 tctcaaaact ttttctattt tcttcttcat atcattttcg gctttaatcg ccaattgcct
    18121 atttggaaat gttttttgat acttttctga tgcaattcct gttatttctt taaacctttt
    18181 tggatatctc agattagttt tatatttccc atttgaaagc tttgtaggca aaatcctcac
    18241 tccttaaaaa aagtaaggca gtatatttca ctaattttag tatatgcggc gagaagaaaa
    18301 tttcaagtta ttttaaatgt attcataaag tctttaatat cttctacgtc aaagcgaact
    18361 acaccaccaa ctcttattac tgggaaatta tatatacgaa tccacttatc tagagtatta
    18421 ttatcgattc ctagaaatgc acaggtttgt tttttattca ggtagactct ggattctata
    18481 aaatcatctc ctaaaatagt agttatgaaa tttttgaatt gctttgggta agcacatagg
    18541 aatttcacct ctccccagtt tacggcgagc cttacggaag tatcattatt tgctgctatt
    18601 gtcatggcat tttgttttcc cagaacaaaa agaaatctct aagtatttgt cgctaatcag
    18661 gtcatggcgt acttgagttt ctagctcgaa tagcagggat cgttattcaa aaattgtatt
    18721 taattgtcaa tgatcaaaaa tagttttttt cctattcact attaataacg ataaacatgt
    18781 ataaaaagac acactacgaa aaaagttaaa tggagtttac tgattttcat atttgtatat
    18841 ggaacagaca tagtcttgaa taatttttat aaatgttttg atgcttatca acttattcaa
    18901 cccgctatcc acttcaagta taatttttcc atacgccaat aaagtagttt taggagcatg
    18961 ttctcatttg agaacgctcc ttttttatat attttgaaag ctacttacta tacgcgtagt
    19021 ttcaaatagt tttgtcattc taatcttcag caacagaaaa aaatctaaac tataaaattt
    19081 agggttgact aaaatataat ttttgtatac catatgatcg tgataatcat tatcatttag
    19141 aaggagaaag ttatgttaga agtgaaaaag cttaatgttt gttataacga ttttttagca
    19201 ttgaacgaga tttctttaaa aatacaagaa ggtgctttaa caggtattat tggtccaaat
    19261 ggtgctggaa aatccacatt gcttaaagca atgttaaata ttatccctca tcagggagag
    19321 gtctccatta ataatgaaga tatcaataaa aaactggtga agattgcata tgtagagcaa
    19381 aaagctgata ttgattttac ttttccaata aaagtaaaag aatgtgtatc tatgggaaca
    19441 tatgccggaa tgaaagtttt tcaaagaatt aaaaatgcag aatggcaaag agtttcaaag
    19501 gctttggaaa aggtcgatat gggaaaatat tctaatcatc aaataggaga attatctgga
    19561 ggacaatttc aacgtgtact tttagcaaga tgtcttgctc aaaatgcgga tttcatattt
    19621 ttagatgaac catttgtagg gatagactta gttagtgaac gaataattat ggatacgtta
    19681 aaaaaattga aacatcaggg aaaaacagtg ctaattgtgc atcatgattt aagcaaggta
    19741 aagaagtatt ttgataatat tattcttttg aatcgaaatc taattgctca cggaagtgta
    19801 gaatctgtat ttaatgaaga aaatttaaaa aaagcctatg gagatactat atttattgga
    19861 gagggggaat aatatgcttc aaaattttat taatggtctg tatgactttc actttttaca
    19921 gaatgctcta attacatctg tagttatagg gattgtttct ggaacagtag gttgttttat
    19981 tattttaaga ggaatgtctc ttatgggtga tgctatttct catgctgttt tgccaggagt
    20041 agcaatttct tatattttag gtattaattt ttttattggt gcgattgtat ttggtctatt
    20101 agcttctatc attattactt ttataaaaag caatagtgta ataaaaggag atacagcaat
    20161 tggtattact ttcagttctt ttttagcact aggagttatc cttataggag ttgcaaatag
    20221 ctcaacggat ttatttcata ttttatttgg aaatatttta gctgtacaag atattgataa
    20281 gtggattaca attggcgttt cggttttagt gttggttata gtagttctat tttttaaaga
    20341 attattaatc acttcttttg atccattaat ggcaaaagca atagggatga atgtcagttt
    20401 ttatcattat ctattgatgg ttctattaac tttagtagca gtaacagcta tgcaaagtgt
    20461 gggaactata ttaattgttg caatgttaat tacaccagca gctacagcat atttatatgc
    20521 aaatagcttg aaaactatga tatttatttc tgcaacagtc ggagcaagtt catcattgct
    20581 tggattgttt attggttata gttttaacat agcagcagga tcaagtattg ttttaacagc
    20641 ggcattctta tttgtaatta gttttctaat ctcaccaaaa caaaaatttt taagaaagaa
    20701 ggagagatcg ttatgatgag aaaatggaaa gcagtactgg gaagtctggg gattttgatt
    20761 gctcttttta tattcggggc atgttcaata aatagtaaag acaaagataa agtggcttcg
    20821 aacgaaaaat taaaagtagt agttactaat tcgattttag cagatattac cgaaaatata
    20881 gcaaaagata aaattgattt acacagtatc gtacctattg ggaaagatcc ccacgaatat
    20941 gaacctttgc ctgaagatgt tcaaaaaact tcaaaagcag atttgatttt ttataacggt
    21001 gttaacttgg aaactggagg aaatgcttgg tttacaaaat tagttaaaaa tgcgaacaaa
    21061 gaggaaaaca aagactattt tgcagcaagt gatggcatag atgttattta cttagagggt
    21121 cagagtgaga aagggaagga agatcctcat gcttggttaa atttagaaaa cggtattatt
    21181 tacgctaaaa atattgaaaa acagttagcg gaaaaagatc ctgataataa aaaattctat
    21241 aaagaaaatc tagataagta tattgaaaaa ttggattctc tagacaaaga agctaaatct
    21301 aaatttgctt caattccgaa tgataaaaaa atgattgtta caagtgaagg atgctttaaa
    21361 tatttctcga aagcgtataa tgtgccttct gcttacattt gggaaatcaa cactgaagaa
    21421 gaaggaacac cagatcaaat aaaacactta gttgaaaaat tacgcacaac aaaagttccc
    21481 tccttattcg tagaaagcag tgtggatgat agaccaatga aaacagtatc aaaagatacc
    21541 aatattccta tctattcaac gatttttact gattcaattg cagaaaaagg acaagatggt
    21601 gatagttact atgcgatgat gaaatggaac ctggataaaa ttgctgaagg cctttcgaaa
    21661 taatttggag ggtaggtatg gtacaaatta aaacgactac tatactaatt tatttactag
    21721 caattcagga attatcaaaa agaaaaaaga aattaaaaaa taatgattta gcaaaagttc
    21781 taagtgttag tccagcatct gtaagtgaga tgctggctaa acttagatat gaggactatt
    21841 tagatatagg atttcaatta actgataaag ggaaaaaatt tattgacgat tacaaaaaag
    21901 gttttaagta aaagacttta tttttaggga ggataagaaa atggctattg gagtgattat
    21961 gataggactt atcctaatag ctggatataa tttttttaaa tctttctcgg attattttag
    22021 aaaaggtgaa tgatttagat gagtctatta attcttgtag gattgctttt tgtatcagtt
    22081 attctgctct ttctcattag tttagctcta aggaatgagt tcagtaataa acgttaacga
    22141 ttgaaacttc aagtcctgtg taagataata aagttaattc atcttattag gaggaagtac
    22201 ccatagaaac cagagattat attataactc accaaaagtt aatagaaagt ggactaagcc
    22261 attttgaaaa agaaggattt cagcaaacta agataaggca actgtgcctg accgcaggcg
    22321 taactacggg tgctttttat aaacatttta cttctaagga agcgttattt gaagaaatta
    22381 ttcagccgca tattaatgcg
//
LOCUS       DAYYKC010000049        22316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000049 DAYYKC010000000
VERSION     DAYYKC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.049"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..906
                     /locus_tag="ACHBBW_001713"
     CDS             16..906
                     /locus_tag="ACHBBW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9153390.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(979..1803)
                     /gene="nadE"
                     /locus_tag="ACHBBW_001714"
     CDS             complement(979..1803)
                     /gene="nadE"
                     /locus_tag="ACHBBW_001714"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9153391.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1815..3287)
                     /locus_tag="ACHBBW_001715"
     CDS             complement(1815..3287)
                     /locus_tag="ACHBBW_001715"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9153392.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3924..4286)
                     /locus_tag="ACHBBW_001716"
     CDS             complement(3924..4286)
                     /locus_tag="ACHBBW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9153393.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4509..5261
                     /locus_tag="ACHBBW_001717"
     CDS             4509..5261
                     /locus_tag="ACHBBW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9153394.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5401..6324)
                     /locus_tag="ACHBBW_001718"
     CDS             complement(5401..6324)
                     /locus_tag="ACHBBW_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153395.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6339..6611)
                     /locus_tag="ACHBBW_001719"
     CDS             complement(6339..6611)
                     /locus_tag="ACHBBW_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153396.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(6955..8142)
                     /locus_tag="ACHBBW_001720"
     CDS             complement(6955..8142)
                     /locus_tag="ACHBBW_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9153397.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(8555..10180)
                     /gene="groL"
                     /locus_tag="ACHBBW_001721"
     CDS             complement(8555..10180)
                     /gene="groL"
                     /locus_tag="ACHBBW_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9153398.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(10231..10515)
                     /gene="groES"
                     /locus_tag="ACHBBW_001722"
     CDS             complement(10231..10515)
                     /gene="groES"
                     /locus_tag="ACHBBW_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9153399.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10740..11402
                     /locus_tag="ACHBBW_001723"
     CDS             10740..11402
                     /locus_tag="ACHBBW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9153400.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(11478..12770)
                     /locus_tag="ACHBBW_001724"
     CDS             complement(11478..12770)
                     /locus_tag="ACHBBW_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9153401.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12940..13569
                     /locus_tag="ACHBBW_001725"
     CDS             12940..13569
                     /locus_tag="ACHBBW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9153402.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(13672..14481)
                     /locus_tag="ACHBBW_001726"
     CDS             complement(13672..14481)
                     /locus_tag="ACHBBW_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9153403.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14536..15405)
                     /locus_tag="ACHBBW_001727"
     CDS             complement(14536..15405)
                     /locus_tag="ACHBBW_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9153404.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(15406..16722)
                     /gene="yycH"
                     /locus_tag="ACHBBW_001728"
     CDS             complement(15406..16722)
                     /gene="yycH"
                     /locus_tag="ACHBBW_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9153405.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(16719..18554)
                     /gene="walK"
                     /locus_tag="ACHBBW_001729"
     CDS             complement(16719..18554)
                     /gene="walK"
                     /locus_tag="ACHBBW_001729"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9153406.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(18559..19263)
                     /gene="yycF"
                     /locus_tag="ACHBBW_001730"
     CDS             complement(18559..19263)
                     /gene="yycF"
                     /locus_tag="ACHBBW_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9153407.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(19453..20313)
                     /locus_tag="ACHBBW_001731"
     CDS             complement(19453..20313)
                     /locus_tag="ACHBBW_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9153408.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(20300..20869)
                     /locus_tag="ACHBBW_001732"
     CDS             complement(20300..20869)
                     /locus_tag="ACHBBW_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9153409.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            21250..21322
                     /locus_tag="ACHBBW_001733"
     tRNA            21250..21322
                     /locus_tag="ACHBBW_001733"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21283..21285,aa:Lys,seq:ctt)
     gene            complement(21604..22296)
                     /locus_tag="ACHBBW_001734"
     CDS             complement(21604..22296)
                     /locus_tag="ACHBBW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9153410.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
BASE COUNT         6425 a         4650 c         3790 g         7451 t
ORIGIN      
        1 taagaggtga tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca
       61 ttagctagta aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc
      121 aaagatttgg caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag
      181 ctagaggaag caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt
      241 cgtgtcgata aaatcgatat cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa
      301 gaaacatcga tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta
      361 gctacgatcc atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga
      421 aggatggatg cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat
      481 tttttaaata cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa
      541 tttccttttt caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag
      601 ctaggaacat ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat
      661 tggtatccag ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac
      721 ttgacgaata tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag
      781 caagacactt ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt
      841 actttatttg gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac
      901 agctgatatt tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg
      961 gctgtttttg ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg
     1021 tttatgttgt gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc
     1081 ttttccttct agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc
     1141 aatcattggt ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg
     1201 tgcattcaat gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa
     1261 aatgtcggct ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg
     1321 atctgtccct aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg
     1381 ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa
     1441 tacttgtact tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg
     1501 gataaagtca agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg
     1561 aacagcgata aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag
     1621 ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt
     1681 tttaagaaac ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc
     1741 ttcttttgga tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct
     1801 catttattct cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc
     1861 atgatagcca ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga
     1921 ttcaagtctc gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg
     1981 atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa
     2041 actgggcgag ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac
     2101 atgaagatag cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca
     2161 gatttcccat caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact
     2221 ttttctgcat tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc
     2281 gatactagct tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc
     2341 ccaacacccc atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca
     2401 tccaagtcat tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc
     2461 cgaacacgct tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg
     2521 attttgtcac ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta
     2581 tcataagtgt cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct
     2641 tcataatcat ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc
     2701 ccaaatatct tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct
     2761 ctagttcccc agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt
     2821 ggatcttctc cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa
     2881 ttgaccatat tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact
     2941 tggattaatg gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct
     3001 ttaaattcaa aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc
     3061 aagtaagcaa tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc
     3121 aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa
     3181 acagcatgta aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat
     3241 agatccgtat gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc
     3301 tcttggcaag ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc
     3361 ttccattttt tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca
     3421 tctgctaaaa ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt
     3481 attcaatgta tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat
     3541 aagtagattt tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac
     3601 caaatgtctc tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct
     3661 tttttgcttc ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga
     3721 aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac
     3781 gtttttaaaa agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg
     3841 agacatcaat cagtctcgcc ttgtttttta ttgtatacat tagatatgga gcaggttacc
     3901 ataaagatga ttattagtcg ttattaaata gaatccttcg tatttttttg ttctatcact
     3961 acaatatctt tatttggacg tttgaattgt attccggcac gttctagctc ctgtttcact
     4021 cctacatgga ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg
     4081 atttcttgcc ttgtgatttt tttcttcgta ttgatcatca cgacttgctt tttatcatat
     4141 gtgtctacca atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc
     4201 gtattcccaa aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta
     4261 ttcacaggtg tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc
     4321 caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt
     4381 taaagacaaa aggatttacc ttttatcact taatagtata acataatttc gtgagaaaaa
     4441 aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag
     4501 gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa
     4561 ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt
     4621 atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa
     4681 tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga
     4741 aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct
     4801 tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag
     4861 aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag
     4921 agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata
     4981 tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca
     5041 ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata
     5101 agaagtggtt ctcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa
     5161 tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg
     5221 cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca
     5281 aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat
     5341 gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat
     5401 tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa
     5461 ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag
     5521 ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct
     5581 ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc
     5641 tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag
     5701 tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata
     5761 acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa
     5821 ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt
     5881 tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc
     5941 tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc
     6001 aagatacctt cgctcttttt ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc
     6061 ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt
     6121 ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc
     6181 attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc
     6241 tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt
     6301 tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt
     6361 aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa
     6421 aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt
     6481 ggtatttctt ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat
     6541 cccttgaact cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca
     6601 taagtattca ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc
     6661 atagcaagga gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt
     6721 ctcttatttt aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga
     6781 atggagtcaa gtccacaatc ttgtgtaaaa gttcaacctc atcaaaagca caatgttttg
     6841 cgttgtgaac ctaactgcca tgcctttctt tctgaggaga gaggtcagga gcagtagcgc
     6901 agcgactctt gatgtctctc ctcagaaaga agagactcgc acaggttcat ggtgctaaat
     6961 gttattcgaa ttaaattcta aatattttcg tggagaagcc agccactcag tgtctttttc
     7021 catcaaaagt gttccaatta tccgagtgat ggaattgata tttggaaaaa tccgaatcac
     7081 tcgttctctc cggcgaatct cttcgttcaa ccgttccaac acattggttg tacgaatcct
     7141 tcggcggata ttttcaggaa aagacaaaat ttgaatagca tcttcaaagc cgttttctaa
     7201 aatgtcgcaa gcagcactta attttgaatc acaaccatat ttttccaaga agtgctcttt
     7261 tctttcacgt gtcaattcaa gttcagaggc tttaaagata ctcttcaact cgtcttttac
     7321 atcagaagaa actttctttg gcagcttatc aaaaatattt ctcaaaaagt gggcttggca
     7381 tctttgccag ctcgcaccta aaaagttctt gcgaacagcc ttaactaacc ctttgtgtgc
     7441 atcagaaatg accagtttta caccgaacaa acctcgttga acgaacgatt gaaaaacggt
     7501 atcccaatta tcttccgatt cgccgtcttg aacatcaaaa ccaagaatcg tacgtcgtcc
     7561 atcagaatca atgccgatac caatatatac accttttgag acaatacgat gattttccct
     7621 tactttcata tacagggcat cacacatgaa aaatggatat tccgagcctt ctaggctccg
     7681 gtttctccac tcaaaaacaa atggatccaa ctgtttcatc agatttgaaa cgaatgattt
     7741 agaaacagtc gcaccatccg ttagcagttc aactgtttta gttactttgc gagtggagac
     7801 accagaaatg accatttcaa tcatggcagc gatcaaagct ttttcgcttc gctgatatct
     7861 ttcaaagata gtaggactaa acttaccgtc tctggttcgt ggaacatgaa gttctaacgt
     7921 accaattcga gttgtaaacg aacgatcgta gtaaccattt ctttgtgatt gccgttcttc
     7981 tgaacgttca taggcatttg cctgaatata ttgatttcgt tgttcttcca tcagctgatt
     8041 aaacatgata gtcataagtg tttttgcggt atcattcgca ccgctttcgt ttataattgc
     8101 ttgaatttct tcgctttcaa aagtaaaatg tacttgagtc attgatatca gtcctattcg
     8161 agtatgtttt ctgcaaaaaa cattgtactc ctaactgaga tcaatggctt ttttgatgct
     8221 tcacttttac acaattatat ggacgtaatc gtgaatggat atttactttt aatagataaa
     8281 atattataac tattaaaaat aactattttc attataatta cacacaaaaa agagcgtggg
     8341 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacagc ggaagcagaa
     8401 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt tccgtgaatt
     8461 tcttcttatt tcttggagtt aaacgcttct gttccagcct ctttttatct gtctttcact
     8521 aatgatcagt tgtagataag caagttgctt tttcttacat cataccgccc atcattgatg
     8581 gatccattgc aggagctgct ggcgctgctg gttctggttt atctgcaacg actgcttctg
     8641 tcgttaataa caaggcagaa acggaagcgg cattttgcaa ggcagaacgt gttactttag
     8701 ttgggtctac gatacctgct tcaaccatgt ttacccattc tccagtagca gcattgaaac
     8761 cagtgcctag ttccacgttc ttcaatttat caacaatcac tgatccttca taaccagcgt
     8821 tttcagcgat ttgacgaatt ggttcttcta aagcacgaac aacgatcttg atacctgttg
     8881 ccacgtctcc ttctgcttct acagcagaaa ctttgctgat aacgttcact agagctgtac
     8941 caccacctga gaccattcct tcttctactg cggcacgtgt agcattcaat gcatcttcaa
     9001 tgcgtaattt taattctttc aattctgttt cagtagctgc accaacttta acaacagcta
     9061 caccacctgc taatttagct aagcgttctt gtaatttttc gcggtcaaaa tcagaagttg
     9121 tttcagcaat ttggtttttg atcaattgca cacgagcttc gattgcttct ttttctccag
     9181 aaccttctac gatcgttgta ttgtctttat ctacaacaac tttagaagca tttcctaagt
     9241 tttcgattgt tacatctttc aattctaaac ctagatcgtc agtaattact gtaccacctg
     9301 tcaaaatagc gatatcttca agcattgctt tacgacgatc gccgaaacca ggagctttta
     9361 cagctactac gttgaatgtt ccacggattt tgttcaatac aagagttggc aatgcttcac
     9421 catctacatc gtcagcaata atcaacaatg gacgtgattg ttgcaagatt tgttctaaca
     9481 atggcagaat atcttggatg ttagagattt ttttgtctgt gatcaagata tatgggtttt
     9541 ctagaacagc ttccattttg tcgttatctg ttaccatata ttgtgataag taaccacgat
     9601 caaactgcat accttctaca acatctagtt ctgtttcgat cccttttgat tcttcgattg
     9661 tgatgacacc gtcgtttcca actttttcca ttgcatcagc aatcaaatgt ccaactttat
     9721 cagaacccga agaaacagct gctacttgtg caatggcttc ttttgaatca acaacagttg
     9781 agatattatg caattcttcg actgctgctt ttgttgccaa ttcgatcccg cgacgaatgc
     9841 ctagaggatt agcacctgca gttacgtttt ttaaaccttc gcgaacgatt gcttgtgtca
     9901 gaacagttgc tgtcgttgta ccgtctcctg caatatcatt tgtttttgat gctacttctg
     9961 atacaagttt tgcacccatg ttttcaaaat gatcttctaa ttcgatttct tttgcgatcg
    10021 tcacaccatc atttgtgatc aatggagagc cgtaagattt ttctagtacg acatttcggc
    10081 ctttaggtcc taatgttact ttcactgtat ctgctaattt atctaccccg cgtaacatag
    10141 ctgcgcgtgc gtcttctgca aatttcaatt cttttgccat gattgattca cctcattgat
    10201 tatttttgat tattttttta tcttccaaat ttactcaaca atcgccatga tatcttttcc
    10261 tgcgacgatc agatattctt ttccttcgta ttttacttct gtaccggcgt atttttcgaa
    10321 cataacttgg tcgcctgctt ttacagcggc aggaactttt tcaccgtttt ctaacaaacg
    10381 gccttcaccg actgcaacaa ctgtcccagt ttggggtttc tctttagcag cagaagctaa
    10441 aacgattcct ccgacagttt tttcttcttc ttttgcgact tcgataatga cgcgatcacc
    10501 taatggtttt aacacgataa atccctccga taactctttt tttaatctgt tagcactctt
    10561 aaccttcgag tgctaaccta catttttaat aatactcatt ctccctcttt tttgcaagct
    10621 tttaaatcaa agaaaagaaa ttttattctg acagctgctt attgaaatta tgatataatt
    10681 gaaatcaatc gatataagct tgccttgcat gcttgttatc ccttaaaagg aggaattaca
    10741 tgtctttaaa aaaatatagc ttcctcagca ttttttgcta tggtctaatt tttatctcac
    10801 ccttgatcct agcttcgata ggacttgtaa aatctacgtc tgaattgatc acagcaactg
    10861 cagtcatgta tatactaggc gccgttgtcc tagctatttt ttattttaag caacgtgaac
    10921 ccttagcgat cgaatcagcg gtaaaaaaag cttctgctcc aaagattgtt atttatggat
    10981 tggttggaat ctttgtcgca ttgattctgc aaagcatcgc tgtcatgtta gaatctgtat
    11041 tgtttggcaa agcaacccct tccgagaata cacaaaatat catccagatg atcatggaag
    11101 cacccgcttt tattttggcg acaactgttg ctggacccat catggaagag ttcgtattcc
    11161 gccgcagtat actggggatc atcagtcgat attcaaattt ttgggtcgga gcagtgatca
    11221 gctctttgct gtttgcgttt gctcataatg atggtcatct gctaatctac tttttcctag
    11281 gtttcttctt cagcctagag tacaaagcaa ctggtcggat ctggacatcg atgatcacac
    11341 acgtaggaat gaacacatta gttgttcttg ttcaattagc tgtccaaaaa gggctagtat
    11401 aaacatgact ataaatactc ctgtcatcct agattggtga ttttcccaat ctaaagcgac
    11461 aggagtattt ttgtttttta gtcgttcatc ttttctgctg ctccatcatt ttttgtctgt
    11521 ctttcctttt tgcagtttgc agaatctatt tttttcaaaa atgggaaata aatgaaaaaa
    11581 ctaattgaca acacgatacc ttgtaaaatc gctgcaggca ctccgcctac taagaaaccc
    11641 gagaggatcg gcggtgttgt ccaagggact tggactgctg taaataaagg aacgaatcca
    11701 aaatataaag cggaataggt aatcaatcct gaaacgaccg gtgcgaaaat aaaaggaaca
    11761 gccatcatcg ggttcatcac gatcggtgtg gaaaaaataa ttgattcatt gatgttgaaa
    11821 aaggctggta aaagagcaag atttcctaat tctctgtatt tcttcgattt cgcaaaaacc
    11881 gtcataaaaa atacagcccc taacgtcaat ccagcacccg taaccgtcat gaactgatcg
    11941 agaaattgct gagtaacgat atgtccgcca tttgccaccg tcaactcttg tcctgaggct
    12001 aagatttctg cattcgctaa tgaattcgct tgcaagacgg gactaaccaa cccattgacc
    12061 atcgcagatc catgaagacc aaataaccat aaaaacggaa caaaaaaacc taataaaagc
    12121 acaccaaaca acgtgtctat gaacgattga atgggaagct ggacatatgt ccatactcct
    12181 tgaacaacag tcattctgaa caactgttga aaaaccgctg aaccgataag cgtaaaagtg
    12241 actataacaa tgattggaat caatgaagta aaagactgcc aaacattttc aggtatcttc
    12301 tctctttgct tccacccttt cgcaagtctt actaaacgtg catagcccca tccgatgatg
    12361 agcccagttg taactgctcc tagtatccct tcagcacctg tccagacagt atctatctga
    12421 gtatttagtg gctgaataag cagtagataa gcagacaagg ctgttatgcc gactggtaag
    12481 cgatcgatgt cttcttcttt tgcataagcg tatgcaatgg caaaaacgat ccaaaaggct
    12541 atcacatcaa atgtagcctg gtatacttgt gtaaaataca tagaaaatcc tgttttgtct
    12601 atccactgag cgatgtaatc cacaggaaaa tgagcaaata agaaaaacag cgagccaaca
    12661 ataattattg ggatcgtgta agtcattcca ttacgcaaag cagttaaggg tctaagattc
    12721 acaacagatg ttatcattgg taaaaaggca cgatctaacc actctttcat ttcttatctc
    12781 cttcaacttc tatttttatc aatcagtttt cttaatttaa ctatacccaa tgatcacgaa
    12841 ccatgaaagg aatgcgcact gagcttttca aaaatcggat tctttgacga atattcagca
    12901 actgcttaca atggacagga ataaaaaagg aggtttttta tgaaaattgc actcattgga
    12961 gctactggcc atgcaggttc atttatttta gacgaagcat tgtccagaga tttagacgtg
    13021 acggcaatcg tgcgtcatcc agaaaaattg ccatcagatg ttccgtttat acaaaaggat
    13081 ttatttgagt tgacaactac tgatcttgca tcttttgatg ttgtcgttga tgctttcaac
    13141 gctcccaaag gaaaagaaga aatgcatcaa acttctttgt cccatttgat tgagatgctt
    13201 gaaggaaccg atacacgctt aattgtcgtt gggggtgcct cttcgttatt cttagataca
    13261 gaaaagacga ctcgtatgat tgaccaagta cctaaaaatg cacccttcta tccaacagct
    13321 ttcaatatgt acgaaggatt gttaaaattg aaacaatcta aaaatctaca ttggacttat
    13381 atcagtccag cttctttatt tgatccacat ggcaaacgta caggtactta tttgttaagt
    13441 gatgattatt tacaaagaaa cgctgctggc gacagtacga tcagcatggc agattacgcg
    13501 attgcattag tagatgagat actggatgag aaacatgaaa agcagcacat cagtgtagtc
    13561 agtcgataat acggttattt ttttaatatg ctaaaaaaat aaaaatagaa gataaaacca
    13621 accctgattg attttatctt ctgcttttta tatttttatt gattgaactg tttaaatcga
    13681 aaatagctca gttgcttcat ctggatctgt atcatagatt tcaaaatcat gtccaacgcc
    13741 aaaatctttt tctttcaaaa cattttccat tgttaaatga gcaagttctt tcatattgtt
    13801 ttccttgcta agatggccaa gatagattcg tttggtccga ttaccgatca catcagtcat
    13861 gacaagtgca ccgtcttcgt tggaaagatg tcctctatct cctaaaatac gctgttttaa
    13921 attccacgga taaggtccca tgcgtaacat ctcaagatca tgattacttt ccatcaaata
    13981 accatcggca ttttcaatga cacctcgcat ataatcactg caatatccgg tgtccgtcaa
    14041 catgacgaat gaccgatttt ctttatggaa acgataaaat tgcggagccg ctgcgtcatg
    14101 ggagacgccg aaactctcaa tatcgatatc gccgaatgtt ctgactttcc ccatctcaaa
    14161 tagatgtttt tgttccactg gtacatttcc aatcagtggt tccatcgctt cccaagtctg
    14221 ctgattggca aaaacatcca aatgatactt acgagccaag acacccacgc catgtatatg
    14281 atctcgatgt tcatgcgtca caagtattcc atctaattgg tccggtgtcc gatcaatttg
    14341 agccatcaag gaagtgattt ttttaccgct aagtcctgca tcgaccaaaa gccgtttttt
    14401 ttcactttcg atataaagtg aattacccga acttccactt gctaggatac tgattttaaa
    14461 agctggtttt tcttcagcca tttgactcct acttcccttc ttactttgta aacttttttc
    14521 attatactcg cttttttatt gatcttccac ttttggtaca gtattgtttg tcacgatcgt
    14581 attgctgaaa gcgttgacac tctcaatttg gaccacttta tcgcttgttt cgatcgctac
    14641 atgccaaaca ggaacataca catttttttc ccggactttc agaatacagg aataagctag
    14701 ctgacgccat aaaatttttg atcctcttgg tattttgtta ttgatgtaca atgtattgat
    14761 tgcttcctct tccgtataca gttccgtctt ttcgcgcagc tgctcgatat cagataaatg
    14821 cgtctgcatg tatttcgtga tgctataatc attatcgttt ttttctaaag aaagaacgat
    14881 ttttgctgta tcatcattga atgggatgcc ttcatactct tggcttgctt ggatttctag
    14941 tggttcttct tctaaatgag aaaagttttt cgtgtacaca taatcattac cgaataaaat
    15001 attgtctttg ttagataaaa aatcttccaa ggaactacta attttatctg gatcgatcac
    15061 gttcttttca gcatctgaac tggataaaga tttcgtcaaa acatcaccgt ctaaaaacac
    15121 accacttgcc agcaagccgc ttttaccagc cttgcgataa ttcgctaatg aagtgctaag
    15181 attggtctgc tccgcgctaa gataatagcc ttgcttgttc tcagacgaaa tcttccccgt
    15241 gtaagtgata tcgtcgctag ccagacgttg ttggattgat tcagtctgat tagagcgaat
    15301 gacattgttt tcttggtata ccgcgtcatg ataactgctg aataaaaaca cattcaaccc
    15361 taaaaaagcg acgaaaaata tccattcgat ttttttgaaa tccaattaat tcgcctccaa
    15421 tccaggtaat ttttccagaa ggtcgttagc tgaataccag ttatctttat atttcacata
    15481 ccattcaggt aagagatcaa cgacacgatt cgtttctttg acattttgcc atgtatagcc
    15541 aacgatcact gaaccaatct tgttgctatc tgctccctta gcttttaact gctggatgat
    15601 ttcattcgta cttgcaagtt ctacttcttc gtctgaaggt atcggcactt gaatcgtatt
    15661 caaactggtt tggatattca cttgcacaga accattcgtt tcaccgccaa cttccacacc
    15721 aatctgtcct ctgctgtctg tcccaaaaac cgggaagcct tccacgaata tccgataatc
    15781 gatcgtattg tcactgcggt caaaatatct gaggttccct aaagaactgc ctaatttgct
    15841 aatatagggg aaactttcag aaaatacact agcattttct tttttctctg gcagctctcc
    15901 acgaaaatca actacttgcg tttccgaatg gatagacatt gtttcattcc caccgtaaaa
    15961 aatcaattca ttgctttcat cattattttt cacgctatct ggtttctgga aaaaagcatt
    16021 tctaaaaagt gtatagggtt gctgtgacag gatataacta tactttttca gtttgatcga
    16081 atgtttcgta gcatattgcg tgtcgatcgt cgatggttct gttgtcattt ctgtccattt
    16141 tgtgtctttt gctgttaatt ctttattgat atctgtccaa ttcaacgaaa tatttgcttc
    16201 atatacgaga tggttcgaat aattcaaaaa tcggattttt ttatttcctt gatcgaattg
    16261 tattctagta aaatacatat tttcgctttc ttttgccaag ttcgacacat ctaaatctaa
    16321 atcaaatgct tccttgtatt ctcttagcaa aaaaggcgca ttataggcca actcaaaacc
    16381 agagttcaga tttttcacct gttcaaactg tgatttgtct cctgaaacta cttcagaaag
    16441 ctttccaaat cttgctttat cgatgatcaa ttgcaatcgg tacacgagat tttcactgtt
    16501 ggtttcttgg atcattcccg aatggtgcca tgtaacatga agcggaagaa agacttcgga
    16561 agtttttcga ttgctttgag actcgatcat ttgagtgttt gtctcatcta cgttgattga
    16621 actttttcct gctgggctca gccagattgc ataagagaag tatatgctta acgcaaccat
    16681 caaaatgagt cctatacgaa cgactttttc agagattctc attcccacca ttcctcctca
    16741 taaggttcat atggcagcga gatataaaaa gtcgaccctt ttccttcctt gctctctgcc
    16801 caaatagcgc ctcgatgcgc cttaatcact tctctagaaa tagctaatcc aagtcctgtc
    16861 ccaccttgtg ctcttgcacg tgctttatct actcgataga accgctcgaa cactcggttc
    16921 agatcttttt taggaatccc caatccttga tcggtgatac ttaaaaccac gttattatgc
    16981 gtttctaata agcgacaagt aatcgttccc ccatctggtg aatacttgat cgcgttattc
    17041 atgatattat cgactacttg gatgatttta tctgtatcta cttcgaccca aagatctctt
    17101 tgcgtaaatt cacgacggat cttataattt ttttcctgat ttcccaccat catatcaaac
    17161 cggtcaagaa caaaattcac taactcgttg aaattgacat attctaattg aagctgggta
    17221 ttacctgtat ccatgcgaga tagatttaag aggtcattga tcattcgaat cattcggtct
    17281 gtttcatcta gcgttacctt caaaaaattt ggtgctactt cctcgtcttt ccacgcacct
    17341 tcgctcaatg cttcgatata actacgcata cttgtcaacg gtgtacgaag ttcatgggaa
    17401 acattagaga cgaattcacg acgttcccgt tctgtttttt cttgttctgt cacatcatgc
    17461 aagacagcta ctaatccgct gataaaacct gattcgcgac ggatcataga aaagtctact
    17521 cgcaagatca tctgatcact ttccaatgta ctggttgaac ggtcaagtac catttcgttt
    17581 ggatcttcta ataatttccg caacgtatat tcttcttcga tatctaaaag tgtcaaaatc
    17641 gattgtccga ttgcttcctc gtttttgata tctagcaagg aaacagccat atcattgatc
    17701 gtaatgactt tacccctgcg atctgtcgca atgacgccgt ctgtcatatg ggatagcacg
    17761 ctatccaaac gattgcgttc agcttccatt gtatcttgag cttcttcaat acgttcagct
    17821 aactgattga aggtatcagc cagctgtcca agttcatctt gtccatgcac tttgactttc
    17881 cgactgtagt ctcctctagc gattcgaatg gcttgttctc tcatttcacc gatcggctgt
    17941 gtaatcgaac gagcaactaa aacagatacg atcattgaaa taaatgccgc aattagtgag
    18001 gcagtaaaga aaatgactgc tgtgttattg atttccgaat atttttgttc gatattactt
    18061 ttgacgtaaa gagccccgat aacggcatcg ccggtcgggg acaggatcgg ttgaacattg
    18121 atattcaccc gtttatcgtc gttatctaaa gcaacataac gtttagtcgt gaaatcattg
    18181 atatcaacat aatcattttt ctttcccaca gcgctttgtt cattgacatc ggttgttcct
    18241 cggatgatcc ctttttcgtc aacgacccgt atttcgatga tatcgctagt cgcactgttt
    18301 tcaataaatc ttttgatatt cgcatttacc tcttcatcat ctgcattatc ttttcgattc
    18361 aattccgggc tgattgttgt tgctaatgat tccaccgttt gattcatatc tttaataaat
    18421 gtattgatcg tggaacgttc taaaccacgt ataaagtagg caccaataat ttcgatcgag
    18481 atcaataaaa tcagaataaa ggtaatagcg atcttaaaat tgactgatcg aaaaaagcgg
    18541 acttttcttt tcatccaatt actcctgttc tgggttacgc aaatagtaac cgacaccacg
    18601 acgtgtcact aaataatttg gatggcttgg attatcttcg atcttttcac gcaaacgacg
    18661 tacagttaca tccactgttc gtacatctcc aaaataatca taaccccaaa ccgtttgtaa
    18721 taaatgttct cttgtcatca cttgtccaag gtgcttcgcc aagtaaaaga gtagttcgaa
    18781 ttcacgatgt gttaactcga ttgtttctcc tcgtttcgtc accatgtaag cttctggatg
    18841 gatcgtcaaa tcaccaatcg tcaattccgc aggtgctgcc tcttctggtt ccttcgcagc
    18901 cgtggcaccg cgacgaagat ttgctttgac acgtgcaact aattctcggt tggaaaatgg
    18961 cttggtcacg taatcatcgg cgcctaactc taagcctagg actttatcaa tttcagaatc
    19021 tttggctgtt accataatga tcggcatatc ataagttttg cgtacctcac gagcaacttc
    19081 taagccgtcc attttaggta gcatcaaatc aagtaaaatc aaatcaggtt ctacctcagc
    19141 aactttttcc aaggcttctt ccccatcata tgcagtataa acatcatagc cttctttggc
    19201 taaattgaat ttgacgatat ccgaaatcgg cttctcatca tcgacaacta aaactttttt
    19261 cataaagagc cacctcttat atattattgt cataccttct cattataccg tatttctgct
    19321 tttgtttcat ctttgcctat aaaacaaaag agaaaattaa aatgaaagtg caaaatactt
    19381 tattattttt tgttctaact tcaataaaaa aggaaaacac tgattttcac ctcagtgttt
    19441 cctttttttg atttatcctt ctaaaacttg tacaccgaca cccaataatc ccggaccagt
    19501 gtggactact aatgccggag aaatctggcc aaagtagatt tggtttgctt gtgggaactc
    19561 tcttttaagt ctcgcttcca tttcaattgc ttcttctttc gcatccccat gggcaacagc
    19621 taagttgaag acctttgcat taccgatttt ttctttgacg taagcaaccg ttttatccaa
    19681 acttttttta cgtcccctag cttttcctac tgtgtaataa atcccttctt cattacatga
    19741 gatgattggg ttaagcttta aagcggttcc tacaatagaa gcaactaaac cgatccgtcc
    19801 acctttttga agatattcca atgtcgcaac attaaagaaa acttttgttc tttcgactaa
    19861 ctcattcaga ttactgatca gttgatccca ttctactcct tcttccagcc attttgctgc
    19921 ttggatcgca gaaaaacctg caccaatacc aatatttttc gtatctagta catatgcttc
    19981 caattcttct tgctgttgtg caattaaacg aaccacatta tatgtcccgc tcaatccact
    20041 ggaaattgtt accgcaagta ccttttcata accgtctttt ttgatttgct caaagatttc
    20101 tgtgatcgac tcaccatccg gcaaagatgt accagggatt tcctctggca aacgttgata
    20161 gatctcttca gaggtaatgt caattttatc tgtatatacc tgatctttat aaataatttt
    20221 caatgggatc atatacatct cgtatttttt tatcaattct tctggcacat ccgtacctga
    20281 atctactaaa attgcgattt tattgttttt catttgtttt ctcctctttt aaatattcgg
    20341 tttctagaac aatcgttttt tccgcaagaa gtttcattgc aaacgataaa gttgccgctc
    20401 ttacagcaat atattctaca ggggttgcct ggtcgaattt ttgcggatga gatttacctt
    20461 gtgctaattg gctgaccatc cacacagctg cttcttgttc atcgcaaaat agattatagg
    20521 cttcacgaat tcctactgtt tttccttgga acaatatccc ttctttgatt tcaggaatag
    20581 tcaatacttg tttcaacgta gtgattgcaa gtaaaaatgc tacgtgttct ttgttatatc
    20641 tttttttaac tggtgctggt atcaaaccta gtttaacgta attgtttacc atagaagagg
    20701 taagcaatgg atgcttttct ccttgtatca caggtgaaag ataacgatca actaacgttc
    20761 tgacttggtc catataaagt tctaagtcag ggagttcttc ccagtgaggc agacggaatg
    20821 tttctagttc cttcccccac tccattaatt gctgacgcat tttttccatc ataaccgctc
    20881 cttttttcat ttcataaaca tgatatcaga cttatgataa acattcaatc tagttgcttt
    20941 aactaggtga tttgattagc gggattccat gaaaacaaaa aacaagaagg ctaagccttc
    21001 ttgtctttta tcgttaagaa caggagaccc gccggggaga cggcgggaaa ggagtaaaaa
    21061 atgaaaaaag tgttagggtt tgttctcttg caagaacttt gtttgttggt atgcttatat
    21121 aatagcgagc aaatgtgaat aatctatgaa tgatttccta taaggaagta aagatttttc
    21181 tgattttctt taaatgtagt cttgcatttt aaaattctta gtgctactat tattaagtac
    21241 tttttgtacg ggccgttagc tcagttggta gagcagctga ctcttaatca gcgggtcgcg
    21301 ggttcgagcc cctcacggcc cattgggtgc caaacccacg tcaccagtac attcttggct
    21361 acttaggtag tttcgaaaag agtgtacgtg gtggcatttt ttatttttcg ggtatgaaat
    21421 tcgggtatgc attttagtat tttatttttt aataacatcc taatcagtca ataaacctgt
    21481 agattatcac agtatgtact tcccctccat tttgagggga gatatcattg tagaatattc
    21541 aaataaaaat tatttagcaa caaaatacta ttttgttaaa gaaatttaaa ctaaaaaatg
    21601 catctatttt cctgtatgtt cagtcacgat ttcttgtgat ctgtttgctg tgtcttctac
    21661 atcgcttttg gctttttcca gtttttcaat ataattattc aaattactgt tttctgattg
    21721 cgctttttct agtttttgtt cagtactttg caattggttt ttaatagtat ttttttcatt
    21781 tgacaattga ttaatttgtt gatttaaact attaacagta ttttgagcat cattaaattt
    21841 attttgttct gtttgtaaac gattattcca atcagtgttt aattgattta tttcagctat
    21901 tttttgattt atttcatcaa ttttagcatt gatttcattt tgtttacctt gcaattcagc
    21961 attttttcct gataattgat tctctaaatt tgttttctct atagttaatt gatttttatc
    22021 attttccaaa gtcaacactt tttgtttata ttcttcaacg ttagcctttg cttgctcaag
    22081 ttcttgatta tttgaagata atcgtgaatt taaactacta atattttgct ctttattttc
    22141 tagtgcttta tctaatgact ccaaattatg attgatttgt tcaatattat cttctccacc
    22201 ccataacaaa atgtcatcgg caaagttttt ccctgcgatc cctgctaaca ctaatccacc
    22261 tataattaat ccacttacta caattttctt attcatatca tcattctcct taacat
//
LOCUS       DAYYKC010000050        21813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000050 DAYYKC010000000
VERSION     DAYYKC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.050"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHBBW_001735"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHBBW_001735"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9153411.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHBBW_001736"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHBBW_001736"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9153412.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHBBW_001737"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHBBW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9153413.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHBBW_001738"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHBBW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9153414.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHBBW_001739"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHBBW_001739"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9153415.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHBBW_001740"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHBBW_001740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9153416.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACHBBW_001741"
     CDS             complement(8557..9027)
                     /locus_tag="ACHBBW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9153417.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACHBBW_001742"
     CDS             complement(9073..9498)
                     /locus_tag="ACHBBW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9153418.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACHBBW_001743"
     CDS             complement(9565..9834)
                     /locus_tag="ACHBBW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9153419.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACHBBW_001744"
     CDS             complement(9845..11440)
                     /locus_tag="ACHBBW_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9153420.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHBBW_001745"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHBBW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9153421.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHBBW_001746"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHBBW_001746"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9153422.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACHBBW_001747"
     CDS             15906..16880
                     /locus_tag="ACHBBW_001747"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9153423.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACHBBW_001748"
     CDS             17050..18450
                     /locus_tag="ACHBBW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9153424.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACHBBW_001749"
     CDS             complement(18528..19250)
                     /locus_tag="ACHBBW_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9153425.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACHBBW_001750"
     CDS             complement(19329..19835)
                     /locus_tag="ACHBBW_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9153426.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACHBBW_001751"
     CDS             19958..21757
                     /locus_tag="ACHBBW_001751"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9153427.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6053 a         4752 c         3911 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DAYYKC010000051        21546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000051 DAYYKC010000000
VERSION     DAYYKC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.051"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHBBW_001752"
     CDS             complement(296..1024)
                     /locus_tag="ACHBBW_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153428.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHBBW_001753"
     CDS             complement(1002..2576)
                     /locus_tag="ACHBBW_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9153429.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHBBW_001754"
     CDS             2805..3743
                     /locus_tag="ACHBBW_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153430.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHBBW_001755"
     CDS             3755..4666
                     /locus_tag="ACHBBW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153431.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHBBW_001756"
     CDS             4690..6162
                     /locus_tag="ACHBBW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9153432.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHBBW_001757"
     CDS             6213..6824
                     /locus_tag="ACHBBW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9153433.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHBBW_001758"
     CDS             6854..8767
                     /locus_tag="ACHBBW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM9153434.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHBBW_001759"
     CDS             8895..11876
                     /locus_tag="ACHBBW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM9153435.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHBBW_001760"
     CDS             11873..13534
                     /locus_tag="ACHBBW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM9153436.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHBBW_001761"
     CDS             13574..14506
                     /locus_tag="ACHBBW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9153437.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHBBW_001762"
     CDS             complement(14898..16445)
                     /locus_tag="ACHBBW_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9153438.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHBBW_001763"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHBBW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9153439.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACHBBW_001764"
     CDS             complement(16890..17084)
                     /locus_tag="ACHBBW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153440.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHBBW_001765"
     CDS             complement(17310..18512)
                     /locus_tag="ACHBBW_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153441.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHBBW_001766"
     CDS             complement(18515..18709)
                     /locus_tag="ACHBBW_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9153442.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHBBW_001767"
     CDS             complement(18756..19604)
                     /locus_tag="ACHBBW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM9153443.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHBBW_001768"
     CDS             complement(19809..20159)
                     /locus_tag="ACHBBW_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153444.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHBBW_001769"
     CDS             20337..21200
                     /locus_tag="ACHBBW_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153445.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
BASE COUNT         6765 a         3563 c         4212 g         7006 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattca
//
LOCUS       DAYYKC010000052        19792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000052 DAYYKC010000000
VERSION     DAYYKC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.052"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..307
                     /locus_tag="ACHBBW_001770"
     CDS             113..307
                     /locus_tag="ACHBBW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153446.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            576..875
                     /locus_tag="ACHBBW_001771"
     CDS             576..875
                     /locus_tag="ACHBBW_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153447.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(972..1406)
                     /locus_tag="ACHBBW_001772"
     CDS             complement(972..1406)
                     /locus_tag="ACHBBW_001772"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153448.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1638..2348)
                     /locus_tag="ACHBBW_001773"
     CDS             complement(1638..2348)
                     /locus_tag="ACHBBW_001773"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9153449.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2338..3198)
                     /locus_tag="ACHBBW_001774"
     CDS             complement(2338..3198)
                     /locus_tag="ACHBBW_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9153450.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3203..3841)
                     /locus_tag="ACHBBW_001775"
     CDS             complement(3203..3841)
                     /locus_tag="ACHBBW_001775"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9153451.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3856..4155)
                     /locus_tag="ACHBBW_001776"
     CDS             complement(3856..4155)
                     /locus_tag="ACHBBW_001776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9153452.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4198..5682)
                     /locus_tag="ACHBBW_001777"
     CDS             complement(4198..5682)
                     /locus_tag="ACHBBW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9153453.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5702..6163)
                     /locus_tag="ACHBBW_001778"
     CDS             complement(5702..6163)
                     /locus_tag="ACHBBW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9153454.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="ACHBBW_001779"
     CDS             complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="ACHBBW_001779"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9153455.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7466..8236
                     /locus_tag="ACHBBW_001780"
     CDS             7466..8236
                     /locus_tag="ACHBBW_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9153456.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8509..9336)
                     /locus_tag="ACHBBW_001781"
     CDS             complement(8509..9336)
                     /locus_tag="ACHBBW_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153457.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9904..11139
                     /locus_tag="ACHBBW_001782"
     CDS             9904..11139
                     /locus_tag="ACHBBW_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9153458.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12138..12398)
                     /locus_tag="ACHBBW_001783"
     CDS             complement(12138..12398)
                     /locus_tag="ACHBBW_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9153459.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12425..13726)
                     /locus_tag="ACHBBW_001784"
     CDS             complement(12425..13726)
                     /locus_tag="ACHBBW_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9153460.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14018..14476)
                     /locus_tag="ACHBBW_001785"
     CDS             complement(14018..14476)
                     /locus_tag="ACHBBW_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9153461.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14489..15820)
                     /locus_tag="ACHBBW_001786"
     CDS             complement(14489..15820)
                     /locus_tag="ACHBBW_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9153462.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15831..17273)
                     /locus_tag="ACHBBW_001787"
     CDS             complement(15831..17273)
                     /locus_tag="ACHBBW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9153463.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17260..18024)
                     /locus_tag="ACHBBW_001788"
     CDS             complement(17260..18024)
                     /locus_tag="ACHBBW_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9153464.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18128..19003)
                     /locus_tag="ACHBBW_001789"
     CDS             complement(18128..19003)
                     /locus_tag="ACHBBW_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9153465.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19258..19342)
                     /locus_tag="ACHBBW_001790"
     tRNA            complement(19258..19342)
                     /locus_tag="ACHBBW_001790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19306..19308),aa:Leu,seq:caa)
     gene            complement(19360..19431)
                     /locus_tag="ACHBBW_001791"
     tRNA            complement(19360..19431)
                     /locus_tag="ACHBBW_001791"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19397..19399),aa:Gln,seq:ttg)
     gene            complement(19441..19513)
                     /locus_tag="ACHBBW_001792"
     tRNA            complement(19441..19513)
                     /locus_tag="ACHBBW_001792"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19478..19480),aa:His,seq:gtg)
     gene            complement(19575..19659)
                     /locus_tag="ACHBBW_001793"
     tRNA            complement(19575..19659)
                     /locus_tag="ACHBBW_001793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19623..19625),aa:Leu,seq:caa)
     gene            complement(19680..19750)
                     /locus_tag="ACHBBW_001794"
     tRNA            complement(19680..19750)
                     /locus_tag="ACHBBW_001794"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19716..19718),aa:Cys,seq:gca)
BASE COUNT         6284 a         4092 c         3077 g         6339 t
ORIGIN      
        1 aaaaactttc aaattgattc tttttattta tcataaagat atagatacat aatcattttc
       61 acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta
      121 tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga
      181 atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa
      241 aataaagaac gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga
      301 aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta
      361 agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg
      421 tataccatat gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca
      481 agatgaatca aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat
      541 ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca
      601 gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc
      661 acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt
      721 cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat
      781 ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg
      841 tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt
      901 gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac
      961 gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca
     1021 taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt
     1081 gttttctcag tatcatctgt caataattcg atttggactg gtccataact agtgatcttc
     1141 tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg
     1201 aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata
     1261 atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac
     1321 cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca
     1381 tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta
     1441 ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc
     1501 gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat aaaaaggttg
     1561 tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta
     1621 cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa
     1681 tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct
     1741 ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa
     1801 tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct
     1861 aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct
     1921 cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca
     1981 tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg
     2041 gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg
     2101 gggagatctg aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt
     2161 accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg
     2221 actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat
     2281 cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca
     2341 caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat
     2401 ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata
     2461 atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg
     2521 aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat
     2581 agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc
     2641 ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct gttctttgat
     2701 ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat
     2761 gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct
     2821 ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac
     2881 gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt
     2941 ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat
     3001 acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc
     3061 taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat
     3121 ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg ctttttcata
     3181 aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt
     3241 gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata aaggtataca
     3301 catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt acagaaacat
     3361 taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc caagtttcac
     3421 cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg atgttatgcc
     3481 aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat tcacctattt
     3541 ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc atccagtctg
     3601 ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat ttcgtatcag
     3661 cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt tgtccttcct
     3721 gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact ttcataacat
     3781 ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca
     3841 tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa atttttcact
     3901 gatttctttg actgacatca catttttgat accgataact gtaactcctt ttttctctgc
     3961 ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt ggccagctga
     4021 actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt ttagttcttt
     4081 cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat ttgcacacga
     4141 tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga ttttctttta
     4201 tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat aaccatcagg
     4261 atttttccga tattgtaact gcggaatagc caataaacca atgacaacta cggcaattcc
     4321 agcataacct aggactttca tgattactgt aaacaatggc caagctgttg cccagtcgaa
     4381 catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata atgctgaacc
     4441 gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga atccacctcg
     4501 gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga aaccggctat
     4561 gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact gtcccaaagc
     4621 accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag tagcagctac
     4681 gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga aagattgagt
     4741 tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata ccgcaaaatt
     4801 taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga attgcgcttg
     4861 aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata gcatgagtaa
     4921 cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt tgatatcttc
     4981 aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt tcatccactc
     5041 agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg caaatccggc
     5101 accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg tccaataaca
     5161 acctaaaatc agtcccataa aaatcaatgc ttgtatccta cctagatcag gaaagcaaaa
     5221 cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg tagtgaaaac
     5281 ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa caaatgcaat
     5341 cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta atccagcatc
     5401 cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga accgttcctt
     5461 tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa cagtcaaaat
     5521 aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag
     5581 caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg tcaagatatt
     5641 tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc tcctctatca
     5701 attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta gagatgagac
     5761 gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt tctagatgtt
     5821 cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc tcattctccc
     5881 cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct gttccaaaaa
     5941 caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc acaatataag
     6001 gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca ataatttcct
     6061 tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg atttctttat
     6121 ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc cccttctttc
     6181 ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt caaatcctcg
     6241 ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc cgcctacttg
     6301 ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc aaatttcagt
     6361 gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag gaatcactac
     6421 ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt tgtctgttat
     6481 ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt cattaccatg
     6541 tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc ctcctgatgt
     6601 ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc cttttaatac
     6661 ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta aagctactac
     6721 attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt tttcttcagg
     6781 aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg
     6841 acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat ggatatgtgt
     6901 aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag gctgagtacg
     6961 taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc cttttttcat
     7021 catgccattc ccatgactac gttttcccgt cccacaccat agatcacata aaatattcgt
     7081 tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag caaaagttcc
     7141 aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact caggaaatgt
     7201 ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt gcttgctccc
     7261 cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc catgaaaacc
     7321 cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg attgtttcag
     7381 tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat ccatggatca
     7441 agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg aaaaacgtca
     7501 aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg aattagctga
     7561 atcccttgat gtatctatta gtacgatacg aagagattta aatttattag aagaaaaaaa
     7621 cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga ctgattttga
     7681 tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta aatacattaa
     7741 cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac tggattttct
     7801 tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact ctccgcacaa
     7861 agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag aagcactagt
     7921 gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta tcattggctg
     7981 tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat caacgatcaa
     8041 cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc atcgaaaaat
     8101 cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt tgatcactga
     8161 taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa aaaccattca
     8221 agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca aaaaaatgaa
     8281 taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag gaatcccaag
     8341 atgtatccat ccaatcgata ccatcataat ctattgcttc ttttataacc gttatatgtt
     8401 gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc acacctcctt
     8461 ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt atttcaggtc
     8521 gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt tttttgcgtc
     8581 catatatctt tctaaataat catcttctcc taattgctta tggcaagaag caatttcata
     8641 aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa tacccttctc
     8701 acataattgg attgctttat cataatcctc gattttacgg taataggtgg cgatatcata
     8761 atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt taagtgtacg
     8821 ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca ttgccacgtt
     8881 tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca cttgattaaa
     8941 ataaaataat gctgcatctg gttttttcgc ataaagatga tcaagcagtc cttgataaaa
     9001 taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg tttgtaaaat
     9061 tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat ttattttttt
     9121 cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat tcagcttttt
     9181 gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga tattgctgac
     9241 agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct ttttctttcg
     9301 gtattctttt agtaatgctc catctacttt aatcaaaaca atttccttcc ctttaaaaaa
     9361 ttatttctcc tttatatcat acacaaaaaa aagttctaaa caaccaaaaa tgtttagaac
     9421 agaataaaaa ttttaatttt aaaataatat tcaaattaaa caactggcgt atctgctcct
     9481 tgatttcttt ttgtgttatc tgaataatta tcgtttacag ttataacaca tagtgaaaat
     9541 tgtcatggcg agaaagtcta agaatatttt taaacataac tatttctttc ttacgtgtgt
     9601 ataatgacga cagcacacct atcaattaat ataattattt tattttttta tgttctcatg
     9661 tttattttta atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa
     9721 aattgttgcg tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag
     9781 aagttgtgaa aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg
     9841 ctgtgttttt tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat
     9901 ttcatgaaga caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg
     9961 attgaggttg ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa
    10021 acttttgctc ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt
    10081 acgaaaaaac aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct
    10141 tttaccaaag aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt
    10201 gagcctcgac tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa
    10261 aagattcctt tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag
    10321 catgcgctga tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac
    10381 tggacaaaaa agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca
    10441 caaaaagaaa tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat
    10501 ctcatcacaa cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc
    10561 gttggtccaa tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata
    10621 cagcgaaagc aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat
    10681 ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt
    10741 gcacctattg actatctgct tgatgacatt caatctgact cagaagatct tttaataaca
    10801 gaatatctgt ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg
    10861 gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa
    10921 tttgagcaaa gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca
    10981 aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt
    11041 cgtcagcagg cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt
    11101 caagaaatcg aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt
    11161 ttaatagaga ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa
    11221 agtaaatagt ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa
    11281 tcatccttaa aataaaagat ctgacgctat ccaaatgagc ataggtagcg tctgccattt
    11341 attcttagtc aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa
    11401 agaaagacca taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa
    11461 gcatcgcttt gtagagattt gtgccgctag tcccctcaac aattactaaa tcaaatgtag
    11521 gatttaccac tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca
    11581 atccgatgca tactcctata taaactgcca gcaatcccca catattgagt agatcatgtc
    11641 agaatgttct atgtcgtcag caacgataca ctaaaagtca cgttcaccat gactgattgc
    11701 tattcccagc acaattattt ctatacacgt ttcatgattt gaataaacac tcacatcata
    11761 catattacca ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc
    11821 tatatttgac ttgatcactt tggtagtaga tctactaatt ctaaatccat tgctgataaa
    11881 atttccattt tttcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt
    11941 aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa
    12001 aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa
    12061 atttaagaag caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgttac
    12121 aaccactttt agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata
    12181 aaattgatag caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata
    12241 tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat
    12301 cgattgattt gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat
    12361 aaaaagataa aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc
    12421 aaacttagac agtctggact tcgttttctc cacgactttg ttcatctgac agcatctttt
    12481 tatcataagc ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga
    12541 tcaagataac aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta
    12601 atgtccatgt tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac
    12661 tgataatagt caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca
    12721 tcataggagc tccaaaaatt atgggctcat tgatattgaa gataccagga ataatagaaa
    12781 aacgaccaac ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca
    12841 cgatcgttgc tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga
    12901 tatagggtaa ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca
    12961 tgatcagcca cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg
    13021 accacaacaa atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa
    13081 tattggttaa aggctcgaat aacgataata aggcagcgtt tacatcaaag cccgcgacgc
    13141 ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac
    13201 gggcaacagc aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga
    13261 aacgatatac ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc
    13321 ccaaagaact cataggtaag acccagccaa gaggtgcctc ttcaggagtt aaaacgttta
    13381 caggaacttg taacataaag aacgttccta aagataatat accacctgtc acactatcta
    13441 atccatatga tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc
    13501 ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata aggggcaata aattcattcc
    13561 atccaggaaa cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca
    13621 atgttgaaac aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca
    13681 ttggtgggac aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt
    13741 ttcaataaaa tatgaaccat tcattgcatg acaacgcaaa gaatgttcat aactaattat
    13801 ataattaaga aattttattt caatatattc tcttaaataa tcaaatttat ttttaagaat
    13861 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
    13921 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
    13981 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
    14041 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
    14101 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
    14161 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
    14221 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
    14281 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
    14341 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
    14401 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
    14461 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
    14521 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
    14581 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
    14641 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
    14701 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
    14761 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
    14821 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
    14881 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
    14941 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
    15001 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
    15061 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
    15121 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
    15181 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
    15241 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
    15301 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
    15361 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
    15421 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
    15481 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
    15541 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
    15601 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
    15661 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
    15721 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
    15781 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
    15841 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
    15901 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
    15961 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
    16021 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
    16081 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
    16141 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
    16201 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
    16261 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
    16321 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
    16381 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
    16441 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
    16501 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
    16561 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
    16621 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
    16681 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
    16741 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
    16801 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
    16861 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
    16921 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
    16981 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
    17041 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
    17101 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
    17161 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
    17221 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
    17281 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
    17341 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
    17401 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
    17461 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
    17521 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
    17581 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
    17641 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
    17701 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
    17761 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
    17821 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
    17881 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
    17941 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
    18001 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
    18061 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
    18121 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
    18181 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
    18241 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
    18301 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
    18361 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
    18421 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
    18481 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
    18541 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
    18601 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
    18661 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
    18721 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
    18781 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
    18841 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
    18901 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
    18961 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
    19021 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
    19081 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
    19141 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
    19201 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
    19261 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
    19321 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
    19381 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
    19441 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
    19501 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
    19561 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
    19621 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
    19681 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
    19741 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat ag
//
LOCUS       DAYYKC010000053        19748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000053 DAYYKC010000000
VERSION     DAYYKC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.053"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBBW_001795"
     CDS             6..305
                     /locus_tag="ACHBBW_001795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9153466.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBBW_001796"
     CDS             378..1739
                     /locus_tag="ACHBBW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9153467.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBBW_001797"
     CDS             1761..2021
                     /locus_tag="ACHBBW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153468.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBBW_001798"
     CDS             2035..2787
                     /locus_tag="ACHBBW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9153469.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBBW_001799"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBBW_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153470.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBBW_001800"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBBW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9153471.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBBW_001801"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBBW_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9153472.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBBW_001802"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBBW_001802"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9153473.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBBW_001803"
     CDS             7161..8558
                     /locus_tag="ACHBBW_001803"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9153474.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBBW_001804"
     CDS             8639..9079
                     /locus_tag="ACHBBW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9153475.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBBW_001805"
     CDS             9227..9553
                     /locus_tag="ACHBBW_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9153476.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBBW_001806"
     CDS             9554..10303
                     /locus_tag="ACHBBW_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9153477.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBBW_001807"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBBW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9153478.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHBBW_001808"
     CDS             complement(11265..11540)
                     /locus_tag="ACHBBW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153479.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHBBW_001809"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHBBW_001809"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9153480.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHBBW_001810"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHBBW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9153481.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHBBW_001811"
     CDS             14770..17436
                     /locus_tag="ACHBBW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9153482.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVIIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WYRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHBBW_001812"
     CDS             17800..18552
                     /locus_tag="ACHBBW_001812"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9153483.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACHBBW_001813"
     CDS             complement(18574..19728)
                     /locus_tag="ACHBBW_001813"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9153484.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6291 a         3467 c         4050 g         5940 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaata atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtatc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttat
//
LOCUS       DAYYKC010000054        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000054 DAYYKC010000000
VERSION     DAYYKC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.054"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBBW_001814"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBBW_001814"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9153485.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHBBW_001815"
     CDS             complement(1172..1867)
                     /locus_tag="ACHBBW_001815"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9153486.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHBBW_001816"
     CDS             complement(1880..2596)
                     /locus_tag="ACHBBW_001816"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9153487.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHBBW_001817"
     CDS             2926..3534
                     /locus_tag="ACHBBW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9153488.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHBBW_001818"
     CDS             3859..4182
                     /locus_tag="ACHBBW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9153489.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHBBW_001819"
     CDS             4364..5734
                     /locus_tag="ACHBBW_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9153490.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHBBW_001820"
     CDS             complement(5864..7165)
                     /locus_tag="ACHBBW_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9153491.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHBBW_001821"
     CDS             complement(7519..9510)
                     /locus_tag="ACHBBW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9153492.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHBBW_001822"
     CDS             complement(9654..9920)
                     /locus_tag="ACHBBW_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9153493.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBBW_001823"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBBW_001823"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9153494.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBBW_001824"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBBW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9153495.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHBBW_001825"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHBBW_001825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9153496.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHBBW_001826"
     CDS             complement(14080..14601)
                     /locus_tag="ACHBBW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9153497.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHBBW_001827"
     CDS             complement(14604..15446)
                     /locus_tag="ACHBBW_001827"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9153498.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHBBW_001828"
     CDS             complement(15439..16164)
                     /locus_tag="ACHBBW_001828"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9153499.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHBBW_001829"
     CDS             16340..16636
                     /locus_tag="ACHBBW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153500.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHBBW_001830"
     CDS             17204..18796
                     /locus_tag="ACHBBW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9153501.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHBBW_001831"
     CDS             18809..19549
                     /locus_tag="ACHBBW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9153502.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAYYKC010000055        19619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000055 DAYYKC010000000
VERSION     DAYYKC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.055"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..2067
                     /gene="tdc"
                     /locus_tag="ACHBBW_001832"
     CDS             232..2067
                     /gene="tdc"
                     /locus_tag="ACHBBW_001832"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9153503.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2372..3850
                     /locus_tag="ACHBBW_001833"
     CDS             2372..3850
                     /locus_tag="ACHBBW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153504.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3816..5147
                     /locus_tag="ACHBBW_001834"
     CDS             3816..5147
                     /locus_tag="ACHBBW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153505.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5261..7813
                     /locus_tag="ACHBBW_001835"
     CDS             5261..7813
                     /locus_tag="ACHBBW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9153506.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7820..9259)
                     /locus_tag="ACHBBW_001836"
     CDS             complement(7820..9259)
                     /locus_tag="ACHBBW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153507.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9234..9416
                     /locus_tag="ACHBBW_001837"
     CDS             9234..9416
                     /locus_tag="ACHBBW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153508.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9605..9967
                     /locus_tag="ACHBBW_001838"
     CDS             9605..9967
                     /locus_tag="ACHBBW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9153509.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10154..11011)
                     /locus_tag="ACHBBW_001839"
     CDS             complement(10154..11011)
                     /locus_tag="ACHBBW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9153510.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11131..12042
                     /locus_tag="ACHBBW_001840"
     CDS             11131..12042
                     /locus_tag="ACHBBW_001840"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9153511.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12049..13700
                     /gene="mdcA"
                     /locus_tag="ACHBBW_001841"
                     /pseudo
     CDS             12049..13700
                     /gene="mdcA"
                     /locus_tag="ACHBBW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13802..14101
                     /locus_tag="ACHBBW_001842"
     CDS             13802..14101
                     /locus_tag="ACHBBW_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9153512.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14091..15767
                     /gene="mdcD"
                     /locus_tag="ACHBBW_001843"
     CDS             14091..15767
                     /gene="mdcD"
                     /locus_tag="ACHBBW_001843"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9153513.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15771..16364
                     /locus_tag="ACHBBW_001844"
     CDS             15771..16364
                     /locus_tag="ACHBBW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9153514.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16388..17464
                     /locus_tag="ACHBBW_001845"
     CDS             16388..17464
                     /locus_tag="ACHBBW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9153515.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17489..18358
                     /gene="mdcB"
                     /locus_tag="ACHBBW_001846"
     CDS             17489..18358
                     /gene="mdcB"
                     /locus_tag="ACHBBW_001846"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9153516.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18469..19233
                     /locus_tag="ACHBBW_001847"
     CDS             18469..19233
                     /locus_tag="ACHBBW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9153517.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
BASE COUNT         6278 a         3355 c         4210 g         5776 t
ORIGIN      
        1 cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt tcgctttttt
       61 gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa tctacgtgga
      121 tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg tgttacagat
      181 aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa aatgaaagat
      241 atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt ttataaaatg
      301 ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat tccttcagat
      361 atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac ccgcgaccat
      421 atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat cccatggcat
      481 tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc tttattagct
      541 tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc tatggcaact
      601 tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat gacagacgga
      661 tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg gtacgcccgt
      721 tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt gaaaaaaatg
      781 tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac cgaaagcttt
      841 acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa aaatatccaa
      901 agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa ggcgttagat
      961 atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga ttatcgaatg
     1021 gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat cccaattcta
     1081 ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt agataaaatc
     1141 gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca tgttgatgcc
     1201 gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt tgtgccgtat
     1261 gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt aacgattgac
     1321 aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac gatagatcct
     1381 cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca taaaaatatg
     1441 agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa ggctccagat
     1501 atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc cgcagtctgg
     1561 acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat tggggcttca
     1621 atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac tgttaaagga
     1681 aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa ttgggtattc
     1741 aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa aatgttcgat
     1801 cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc tcatactaca
     1861 tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat gggtctaacg
     1921 aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat catgactcct
     1981 tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa agcaatggaa
     2041 aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat aacaagagac
     2101 atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc ctgtttttca
     2161 tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga tcgttcgttt
     2221 catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat actgggtttt
     2281 gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa gaaaacaaaa
     2341 acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat caaagcaaat
     2401 cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac gaaatattcc
     2461 agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag tattattgta
     2521 cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc aaaaaggtgg
     2581 tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg tctcatggct
     2641 gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg ctccgttgtt
     2701 tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat tagttgtcat
     2761 tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg caaaaatcgg
     2821 cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc tcttattagg
     2881 gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca gtttttcatg
     2941 ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg cgccgatcat
     3001 gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg aaaatccagt
     3061 taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg tcaatatttt
     3121 gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga ataatatcgc
     3181 gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg tgaatctgtt
     3241 tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct caggaccagc
     3301 aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca atttttggaa
     3361 gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca ttatctcaat
     3421 ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga tgctcgtaaa
     3481 ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag ttttgagatt
     3541 gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag agaaaaagag
     3601 caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat tttctgtcgg
     3661 actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca tcagtgtcat
     3721 tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg aacaggaagt
     3781 cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca atgaaacgga
     3841 ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga acaggattat
     3901 ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc gtttatttga
     3961 tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt ttagcaaata
     4021 gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag tgggcatttt
     4081 ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa ttaacagcaa
     4141 taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc ggaggtagcg
     4201 cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt gaattggaat
     4261 cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg ggtataggat
     4321 tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt ggacagatcg
     4381 atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg gttttattca
     4441 gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat ccgcaaaaga
     4501 atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat ataggaacga
     4561 ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa agtcctttag
     4621 tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat ctaatgattt
     4681 tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg attttatatc
     4741 aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag aaaggcgtac
     4801 ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt ctacattttt
     4861 ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg agttgttttc
     4921 tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa aagaaacgga
     4981 acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca attgctttca
     5041 gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc tggtttgtat
     5101 tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc cagatacatt
     5161 ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa aaaacttccc
     5221 tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt acagattgac
     5281 ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc aagaagaacg
     5341 acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc agacaaaacg
     5401 atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga tcattgcctc
     5461 ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat tcgtagtgat
     5521 cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat tgaatggttt
     5581 gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag aaaaaatccc
     5641 tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg tcgttccagc
     5701 agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt tgacgggaga
     5761 atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga tcggcgacca
     5821 gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg gtgtcgttgt
     5881 agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat cggtcgaaca
     5941 acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt ttcaaggcat
     6001 tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta tggattggca
     6061 tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag gattgccagc
     6121 tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa aggcgatcat
     6181 caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt cagataagac
     6241 aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag aagaagcctg
     6301 cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac agaaaacaga
     6361 aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa cagccaatct
     6421 acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat atatggcaac
     6481 gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag aagtattatt
     6541 tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag ctgcagaatt
     6601 tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt cgaaacagga
     6661 actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat tgatcgatcc
     6721 gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga tctcagtgaa
     6781 aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa ttggcttaaa
     6841 acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg aagagttgat
     6901 tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata aactgcgaat
     6961 tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg gcgtaaatga
     7021 tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag gtagtgaagt
     7081 aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga ttgcaaaagc
     7141 cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt tcttcttgcc
     7201 aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc gcccgctacc
     7261 tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt tatcttatgc
     7321 actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag atccaaagca
     7381 aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac tgatcatggt
     7441 tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa ccattcttct
     7501 gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt tgtccaagcc
     7561 gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag gtatccttgc
     7621 tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg gtacagtaag
     7681 tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat tcttagttgt
     7741 agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa cagctagaga
     7801 atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc aaacgattga
     7861 gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga gaatctagag
     7921 ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga tcggttagtt
     7981 cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc tgcaagaacg
     8041 ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc ggtttgatag
     8101 aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt ggttcattga
     8161 tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac aagtgtgcat
     8221 attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct tcaatcaata
     8281 tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga gatgtttttt
     8341 ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg aggacctcta
     8401 aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg ttgtaattcc
     8461 aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga taaatacctt
     8521 ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg ctgttggtat
     8581 ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata aagaaccaac
     8641 atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg tttcttaaaa
     8701 attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg ttatgagtga
     8761 aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca agaatacaag
     8821 ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga ttgattttag
     8881 tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa tcagggacag
     8941 caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat tgtttgaaaa
     9001 aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg attcctgctt
     9061 gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga aggtcagaaa
     9121 aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt atctttgtat
     9181 gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg gtaatgattt
     9241 ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg gagaaaataa
     9301 acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga gcatttactg
     9361 atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa aaataggtgg
     9421 gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag gcggattgaa
     9481 catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac tacttgtatt
     9541 catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa agagatggaa
     9601 gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca atttgtctcc
     9661 agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa aaagcaaaaa
     9721 atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg caagtgacca
     9781 taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa ttttgcgagg
     9841 aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct ctattgatct
     9901 acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt ttaaatcaac
     9961 gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag cttcaagaaa
    10021 taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc ttattttcta
    10081 agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga attttgtccc
    10141 agctcttttt tttttatttt cctaaataaa attcccgcat cgtttttgca atcattttga
    10201 tcaaaggatg atcttctgtg aagctagaag ataaataaat tgttcgtgtc aagggcaaat
    10261 ctttgatttc ccgcaagacg aaaggatcat ttttatcgta agaccaagaa acttttggga
    10321 aaaagctaac agctaatcct tgcttgacaa tagaacgtaa tgtcgcagga tcatctgtcg
    10381 tcgaactgat cgtcaattcg atacttttcg cttcaagtgc tgcatccagt gtatgacgga
    10441 aggcgttgct ctttgacagc atcaagatgg gggtgcgttg gatatcttct aattgcagat
    10501 gttctttttt agcaagagga tgtttcttag gaatggcgac ataaacaggt tctttcatga
    10561 taggaatatt ggtcatgcca ggcttcatct ccgaagtgat gtacagtaca ggatcggtaa
    10621 tatcatctgg taaatgatgt tgcatgatcg ttaattggat atcaggatgc ttttccataa
    10681 aaattcggac caagttcgga atagaaacag aggctacctc taaatacaat atgacggaag
    10741 taacgatctt ctcattgacc gaagacaata atttgattcc ttcttctaat gtgtctaatg
    10801 tcttgactgc atatggataa aattcttctg cataaacggt cgtctcaatg tttctgccgc
    10861 tatggtaaaa caaagggaca cctaattcgt tttccaaatg tttgatcgca cggctcaagg
    10921 caggttgaga aataaataat tctttggctg cttgggtaac attttttgtt tcgaatactt
    10981 ttttaaaata ctgcagatga agcaatttca taattatcca tcccatccat acctttttgt
    11041 tatggttttt tctttttttg gcatttttta ttatagcaga aattgactaa gatagcagtg
    11101 gaaaaagtaa gtgctgagga ggcgaggctc atgagtacag gtttcttatt tcctggacaa
    11161 ggcagtcaga agttagggat gttagcggat atccctgaaa tgtacatgga acgtttagaa
    11221 aatgtgacgg ggtatcggtt agttgataaa gaagaaaact atcaagatac ggtttttatc
    11281 cagctagcat tactgacaaa agcagtattt tatttagatg aaatggaaaa aaacggtatt
    11341 cagcctgata ttgcagcagg acattcgatt ggtgcttttt gtgcagctgt tgcttgtcga
    11401 tctttgagat ttgagaatgc agcggaacta gtctatcacc gcgcagtctt aatgaaagaa
    11461 gcttatccaa gcggttatgc aatgggcgta gtagtaggtg tgacgagaag cgaagctgag
    11521 gaaattgtag caaaaacatt tgatcctgac catccagttt atctgtcaaa tgaaaattgt
    11581 ccgatgcaac atacgttatc tgggagtatc gaaggtttat ccgttacgtt ggcagaagcc
    11641 aaacggcacc aagcgcaaac agcgaagctg ctgaaagtcc ctgtgccatc tcactgtctc
    11701 ttgatgaatg atacggtcaa gaaattcaag ccgtatatgc agtctgtcca tctatctgat
    11761 gctagatgtt tgtatataaa aaatacggat ggaagagcca ctcaggacgc agaagagatt
    11821 cgtaaagatt tgttggaaaa cctagctttc cctgtgcagt ggaaccaaat gatggacttg
    11881 gcaaaagaat taggaatgga tctgacgatt gaatttcccc ccggcaatac tttgacaaag
    11941 ctgattcata caaaatttgg tgtagataaa gggatccgta cgatcaatct agaccaacat
    12001 ggtattgatg acgccacgtt tctatatcaa aaatggagat gactaattat ggaaaaacca
    12061 aaccgcaact gggcttctaa acgcgaaaaa aaagcgcagc gattggaaaa aatcagcaac
    12121 ttgctcaatg gcaagtatgt agaaactgaa aaaatcgtag aagtaatgga aaaactgatt
    12181 gctcccggcg acaaagttgt attagaaggc gacaatcaaa aacaagcaag ttttctttca
    12241 gaaagtcttg aaaaagtaga tgcagaaaaa gtgaatgatc tgcatttgat catgtctagt
    12301 atctcacgac cagaacattt gaatattttt gaaaaaggaa tcgcaagcaa gatcgatttc
    12361 tcttatgctg gtgcacaaag cttgcgtgtc gcccaaatga ttgaagacgg cacaatgaaa
    12421 ttaggtgata tccatactta tttggaactt tatggacgct tattcatcga tttgattcca
    12481 aatgttgttt tagtagctgc agataaagca gataaagacg gcaacttgta cacaggtttc
    12541 aatacggaag aaacaccaac aatcatcgaa gctgcagctt tcaaagatgg tatcgtgatc
    12601 gttcaagtta acgaagttgt cgattcttta ccacgtgtag atatccctgg tgactgggta
    12661 gacgttgtcg tgaaagcaga taaaccttat caattagaag ctttattcac acgagatcct
    12721 caaaacatca cagaattgca aattctaatg gcaatgatgg caatcaaagg aatctacgca
    12781 gaacacggcg tacaatcttt gaatcacggt attggattca atacagcagc tatcgaattg
    12841 ttactgccaa cttatggtga atcactaggg ttaaaaggga aaatcgcgaa gaactgggta
    12901 ttgaacccac atccaacgat gattccagcc atcgaatcag gttgggttga aagcatccat
    12961 agttttggtg gcgaagttgg tatggaaaaa tatattgctg ctcgcccaga tgtctttttt
    13021 gtcggtaaag acggcacgat gcgcagtaac cgaacattat cacaagctgc aggacaatat
    13081 gccatcgata tgtttatcgg ttcaaccttg caattagatt acgaaggaaa ctcttctacc
    13141 gtaacaaaag gacgtttatc tggttttggc ggtgcgccta acatgggaca taatcctggc
    13201 ggtcgtcgtc attctacacc agcatggcaa tcacttcgcg acaatgatga tccattaggg
    13261 aaaggccaaa aattagttgt tcaaatggtt gaaacatatg gttcaaataa aaaaccagtc
    13321 tttgtcgaag aattagatgc actagctgtt caaaaacaag caggcttagc caatgcaatg
    13381 caccagtgat gatttatagc gaagatacta cacatatcgt gacagaagaa ggaattgctt
    13441 atttgtacaa agcaaaaaca aaagaagaac gacaagcagc aattgcagca attggcggtg
    13501 ttacacctct tggaatgact agcacgaaag aatcactaga tagcttgcgt gcagccggca
    13561 tcgtgaaatt accagaagat ctaggcatca aacgtagtga agcgaaacgt tcattattag
    13621 ctgcacaaac catcgatgat ttgatcgaat ggtcagatgg cctgtatgaa ccaccaatga
    13681 aattcagaac ttggtcataa aattggcgag aaaaaccttt tgtcgccatt tacccagatg
    13741 gaagaagaaa gacgaataag cagaacgtgt atgctctgtt ttacaaaaag gggagagatc
    13801 catggaacaa ttagcttata aatatccaac gaaacagccg atcaataaga aaatccatgt
    13861 aggagtggtc ggttcaggag atttggaaat cttgatgttt ccaacagaaa aaacagaatc
    13921 tactgtaaaa atttgtacag gaagcgatgg cttcggcgaa gtatggaaca atgtattaac
    13981 gagatttttt gatcgttatc caatttcagc agatatcgtg atcaatgact tcggtgctac
    14041 accaggagtt gtgtacctac gattgacgca agcaatggag gaactttcag atgaaaaata
    14101 gttttgtaga attaaacgcc agagaacgag tgaacgctct acttgacaat ggacgtgaat
    14161 tgattgatcc attcgatcaa atcatcgcgc caagtttacc agcacaagga atcgtccctg
    14221 aaagtgatga cgggatcgtt gtcagccgtg gcacgatcaa tggcaaacaa gcagtagtaa
    14281 tcgctatgga aggaaaattc caaggtggcg gtatcggtga agtttccggt gcaaaaatcg
    14341 ttgctgcact tgaacatgtg ttagaagaaa ataaaaaagg aaatgagatc tttccagtca
    14401 ttgtactaga tacaggtggt gttcgtttgc aagaagcaaa ctacggactg ttatctattt
    14461 cagaaatcgg aaatgtaatc attgctctaa aacaatatgt accagtcatc ggtttgattc
    14521 caggacgtgt aggatcattt ggcggtatgt cgattacttc agcattaatg agttatttaa
    14581 tcgcaacaaa aaaagcgaga gtcggtttga atggaccaga agttattgaa caagaagcag
    14641 gcgttcgaga atttgattct agtgacaaag acttgatttg gaatacatta ggatctcgtc
    14701 aacgggtaga atcaaaaatc attgatgaat tagtgactga tagtgtagaa gcaatcaaag
    14761 ctgctgtcat cgcagcaatg gacgaacgaa aagacacgca tcgcagtgaa agctcagcat
    14821 tttatttatc attacttgat tctttagatc tttcaaaacc aatggccatt gacgattaca
    14881 atgaagcaat tgctaaccat caagcaaaat caattgactt accagaagta gcagaaggaa
    14941 atgaaccagc tgcagcaagt gttggttacg attggtttgc ggcactaact ggcatgaaaa
    15001 atccaacttc ttctattagt actgtgtatt atggtaaatc aagtaaattc atagaagaag
    15061 ccatcgtgac atcaatcgta cctgatccta aaaacccgat gtaccgtgta cgaaacggtg
    15121 aagttggttt agttgaaggt ttccgcatgg gacaaatttt ggatcacgtt tacgaagaag
    15181 ataaagacaa aaaagtgaaa cggccaatca tcgttgtgat cgatgtacca agccaagcat
    15241 acggctataa cgaagaattg atcggtatcc atgtggcttt ggctaatagt gctgctgctt
    15301 atgcaaaact ccgtcaagct ggacatccag ttattggatt gattgcagga aatgctattt
    15361 caggtgcatt tttagcacat ggattacaat caagccgctt gattgcttta aacaataaag
    15421 aaattaccgt ccaagcgatg tctaaagaaa gtgctgcacg tgtgacaaaa cgttcggttg
    15481 ctgaaatcga agcagctgct gaaaaagttc cagctatcgc ttacgatatc aataattata
    15541 caaaactagg agcgatctat catttcctag aagatatcac cgaccaagct gcttcagaac
    15601 aaaatgtcag caaagtcatc gaagcaatca atgaagccat tcaagatgtc cgcaatacaa
    15661 atgacacgat gctgacaaat cgatacacaa acgaaacagc caaaacattc ggacgtgtcg
    15721 aaacaaataa agtccgtgcc aaaatggacg aagaatggga tgcataaaaa atgattcaag
    15781 cacatgatat tgtccttttt gataaacatg aaatagaagg gttcaccttg cctgaatggg
    15841 tcgttctaac tgaaaagatg ttcggaacag ttcgtcgtgc gtcgacggat cacgcagaaa
    15901 tactaccgat tggtttacgt ggacctgccc gcaatcagag atggtcaaca gaaatctcaa
    15961 aacatgccgt caaacaagtc attcatccgt gggacatcat cgaacaagaa tcgtatagac
    16021 aagagaaaat catggattat cctgtttatc agcaatttca tgctgctaga aacatattat
    16081 caggatgtaa atggggaatc ggcggcagct taggatttga attatcgact gggattccgg
    16141 cagtaaaaga aaccagtgat tttgatttgc tgctgtacgc aaattcaccg attgaattgc
    16201 caattcaggt aattcaatcg catcctgctt tttttgagca attcgatacc caagtagtga
    16261 ctcggcaagg cggtttttca ctaaaagagt atttgagaga gccagagaaa aaaattctat
    16321 tgaaaacagt cgaaggacct aaattaacca aagaactttg gtaataaaga aaaagagaga
    16381 gaaagagatg tttgaaaaca aaaaaatcaa aatcaacgta agtgacttcc agaaagtatc
    16441 agctgtaacg gctgttatga tgcaaacgat attgagcttt gctttggcaa acagccacag
    16501 cgcaacaaat acagcttttc ttggtacctt ttacgtcatc gttaaataca cagcaccaat
    16561 gtttattttt gcaattgttt ataatatggt aaaaacaagt cagcatttgt cttattggga
    16621 atttttgaaa gaaaagtttt ttgaactagt cgttccgtat cttctatgga cgacggctta
    16681 tctttggcta tttccagcag tccaacaaca aactccgtat acggatactg caagcttttt
    16741 actgaaatat gtcacaggtg atggagcgcc tcatttatgg tataccgtta tgatgctgca
    16801 gatccaattg ttcatgccgt tttttgtttg gctcggttac aaagtatttg ctaataaaaa
    16861 atatgtatgg ccagtattga ttggttcaac gattttatat gtcgcatggt atctgttcta
    16921 tcaaacacaa gtattgactg ggccttatca tgacagctgg tacttattag atcggtttgt
    16981 tgctagtttc atgatttatg gggtagccgc atttgtttat cacgaaaaag tctatcaata
    17041 tttggacaga gtacgttatc ttttcttacc tgttgcctta gttatcgcgt tcttttctgt
    17101 ccgttcattg ctcgcacatc caggagactt gagctttgcg aatgcacctt acttgaatac
    17161 gatccaaagt ttatacagtt tagtcatcat ctttgctgtc ttcataggcg cttccaaaat
    17221 gattgtaaac gattcgccaa aattgccatt gttcaaatgg ttgtctgttt atgcttatcg
    17281 tacgtattta gccaatgtat ttgttttcca agtactgtta ttattgttta aagatagttg
    17341 gctgcagtta ccttcaggag tcatgattct tgtagcttat ctaatgacgg caagttgtgc
    17401 atttgcttta agctggttac ttcatatcat ttgggtagct atcaaaaaag gtttcagcaa
    17461 gtaatagtag gaagaaggag tgagtgtcat gataaatgac gaacgaaaaa aagcagtgac
    17521 tcagcaactc gctgcgttgg tagaggaagc attgatcgca gaagtaacat taagtccaaa
    17581 acctggatta gtggatgcac tggatgcggg tgcacattcc gatatggact atgctttatt
    17641 tcttaagtct gctaagacgc taactccttt ttttgaagaa atggctcaaa tagcatggca
    17701 tcatgctatt gatcaggagt taagagaaag aattgctgag attgggcgaa aagcggaaaa
    17761 ggcaatgctg attgctacaa atggcatcaa tacccataaa ggagcgattt gggcaatggg
    17821 gctattgatc agtgtcattg cacaaaaaat cagtatggaa cagccttttt ctttatcgaa
    17881 aatacttgat gatgtcagca agcttgctgc ttttcctgat acaaaatatc gtcataaaaa
    17941 aaccactcat ggtcataggg tgaagcaaaa atatggcgta aatggtgcat atgaagaagc
    18001 gatattaggg tatccgcata tcaggcttgc aattcaagca gctaaggagc tagataatga
    18061 ttcatatgta cagaaacaaa tgcatatgtt gttagttttg atgagttcgc tagatgacac
    18121 atgcgtattg catcgcagtg atcagaaaac gttagtggaa atgcaaaaac ttgctcatgc
    18181 tgctagtcaa agtgctttac caaatcgtcc atttacagca ctgttgcact tttgtcagga
    18241 aaaccaaatc tctcctggag gaagcgcaga tctcttgtct gcaagcttgt tcatgctgaa
    18301 gatagaggaa ttatggactg ccgacacata catttgtccg atgttactca atgactaaat
    18361 aagacaaaat tgctaaaaaa acagtttatg catctggtta ttgactcttc cttttagcaa
    18421 ggctatcata aaaatagttt tttgtaataa aagataacga ggaagacaat gaaaaaaatc
    18481 aattatttag caatcatttt gttagtgggc ctatgtttgt ttgctctgcc gacccaagga
    18541 ttcgctgtcg cgggtggtca tggtggaggt acatctagcg gtcgaagttc gggcagaggt
    18601 tcatttggtg gtcgttcata tgggggcagt tattttggca gtcgttctta cggcagagat
    18661 tcttatgggt attatggtcc aggtacaggt ccttctagtt atgagcaaac gggatttctt
    18721 gcattagctg ttttgtcagt aggaggggtt tctgcttttt ctatcgtcaa gaataaaaga
    18781 caaaagaaaa ggctgaaagc ggagatattg tcccgcatgt caggaacggc taaagaaaag
    18841 aaagcacagt ttctggaaat ccaaaagata ttcatgaaga tccaatccgc ttgggaagaa
    18901 gaaactcctg aaaaagccag aagctgttat acagacaagc tgtttgccga acatcagaaa
    18961 gttctgcaaa aaaacaaagc agacggtgta agaaatcata ctaaaaaaat aactattcag
    19021 gaagtaggaa attatcgaca gatccacgaa aatagttttg ccatccgaat tgatttcaga
    19081 tgctgtgatt attcagtcga tcgaaaagat gggcgattga tctcaggaag taagacacaa
    19141 cgacagtatt tttctcagat atggtatttt gatttttctg agaaagaaaa aagatggcaa
    19201 gccgatttta ttcagccgat tatgctggat tgacaggaaa tatttatgaa cggagttgtg
    19261 acaaaagcct tgtcgcagct cctttttctg aataaacggt gtgccagaga tagcctatcg
    19321 gcaataaggc acgattttgt aaaatatggg aagcgatttt tcaaaatcgc ttcttatcgc
    19381 tcaaggctga acatctctgt cacaacctct ccactaaatg gtgtttaaaa gcttgcatct
    19441 gcgataataa gaacaaccaa acgaaaaata ggaagcacta ttttagttta gttgttctta
    19501 ttacttgatg caggacagct ttttcacaac ctcttttttg tatagatggg caaaattttt
    19561 gtaataatag gcgtatgtct ttttcgttga ggagatgaaa aagcatatgg cttattttt
//
LOCUS       DAYYKC010000056        19235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000056 DAYYKC010000000
VERSION     DAYYKC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.056"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..635)
                     /locus_tag="ACHBBW_001848"
     CDS             complement(39..635)
                     /locus_tag="ACHBBW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9153518.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="ACHBBW_001849"
     CDS             985..1329
                     /locus_tag="ACHBBW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9153519.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="ACHBBW_001850"
     CDS             1330..1749
                     /locus_tag="ACHBBW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9153520.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="ACHBBW_001851"
     CDS             complement(1841..2191)
                     /locus_tag="ACHBBW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153521.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="ACHBBW_001852"
     CDS             complement(2157..3488)
                     /locus_tag="ACHBBW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM9153522.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="ACHBBW_001853"
     CDS             3743..4309
                     /locus_tag="ACHBBW_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153523.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="ACHBBW_001854"
     tRNA            4454..4541
                     /locus_tag="ACHBBW_001854"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="ACHBBW_001855"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="ACHBBW_001855"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9153524.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="ACHBBW_001856"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="ACHBBW_001856"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9153525.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="ACHBBW_001857"
     CDS             complement(8266..8712)
                     /locus_tag="ACHBBW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9153526.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8940..9089
                     /gene="rpmG"
                     /locus_tag="ACHBBW_001858"
     CDS             8940..9089
                     /gene="rpmG"
                     /locus_tag="ACHBBW_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9153527.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9177..9695
                     /locus_tag="ACHBBW_001859"
     CDS             9177..9695
                     /locus_tag="ACHBBW_001859"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9153528.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9772..10728)
                     /locus_tag="ACHBBW_001860"
     CDS             complement(9772..10728)
                     /locus_tag="ACHBBW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153529.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10894..11700
                     /locus_tag="ACHBBW_001861"
     CDS             10894..11700
                     /locus_tag="ACHBBW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153530.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11697..12686
                     /locus_tag="ACHBBW_001862"
     CDS             11697..12686
                     /locus_tag="ACHBBW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9153531.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12683..13687
                     /locus_tag="ACHBBW_001863"
     CDS             12683..13687
                     /locus_tag="ACHBBW_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9153532.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13819..14361
                     /locus_tag="ACHBBW_001864"
     CDS             13819..14361
                     /locus_tag="ACHBBW_001864"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9153533.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14381..15079
                     /locus_tag="ACHBBW_001865"
     CDS             14381..15079
                     /locus_tag="ACHBBW_001865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9153534.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15100..15312
                     /locus_tag="ACHBBW_001866"
     CDS             15100..15312
                     /locus_tag="ACHBBW_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9153535.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15312..16274
                     /locus_tag="ACHBBW_001867"
     CDS             15312..16274
                     /locus_tag="ACHBBW_001867"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9153536.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16292..16687
                     /locus_tag="ACHBBW_001868"
     CDS             16292..16687
                     /locus_tag="ACHBBW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9153537.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16850..17215
                     /locus_tag="ACHBBW_001869"
     CDS             16850..17215
                     /locus_tag="ACHBBW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9153538.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17212..19023
                     /locus_tag="ACHBBW_001870"
     CDS             17212..19023
                     /locus_tag="ACHBBW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9153539.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19085..>19235)
                     /locus_tag="ACHBBW_001871"
     CDS             complement(19085..>19235)
                     /locus_tag="ACHBBW_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9153540.1"
                     /translation="GILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMR
                     KRVSR"
BASE COUNT         6219 a         3279 c         3926 g         5811 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taaaaaacat aatctatata aacaagtgta
     8761 agggtattta tacaaaaaga aaaagatttt gtttgagaag taaaaccgtt gtatttatcg
     8821 tcaagtagtt ttacttaaca aaatcgtgaa aaacactagc aatctagaaa aaatcatggt
     8881 aagataatca agtctgagag tcatcttaga ggattcttgt aaaaatggga gggaaaaaca
     8941 tgcgcgtaaa catcacttta gagtgtactt cttgtaaaga acgtaactat ctaacaagca
     9001 aaaacaaacg taacaatcct gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca
     9061 aagttacttt gcaccgtgaa acaaaataat gtttttaaag ttctcaattc cttgctggtc
     9121 aaagaattga gacttttttt atctcaaaac atctaaaatc ataaggagcg gataatatgt
     9181 ttatttccat gtgggcacaa gataaaaatg gtctgatcgg caaaaatggt ttgttaccat
     9241 ggcgattgcc aaatgatatg cgtttctttc gcgaacatac aatggatcga attttagtga
     9301 tgggaagaaa gacctatgaa ggaatggggg atctttcact tccttatcgc catatcatcg
     9361 tgttgacaac gcaaccaaat tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg
     9421 atgaactttt gtcgtatagt caaacgatct cagaagatat ctatgtttcg ggtggtagtc
     9481 ggatttttca agaacttctt ccttacactg gtcggatctg gcgaacattg atcgatagca
     9541 cgtttgaagg cgatacgtat attgggaata ttgatttttc agacttcgcc cttatagagg
     9601 agtatgaggg gataacggat caagaaaatc tatatgccca tcgttttcaa aaatgggaaa
     9661 gagtaaaaaa tagggttgct tcaaaagaag aataaaatct atgcgtctag tttgttaata
     9721 agaaaaaaag catctcacga ctaatcgtga agattctttt tttagttaaa atcaatttag
     9781 tttttctttg atatcattga ccatttcctg actggcaata ggtccgttat atagccaact
     9841 agattcaggt ccgaattgca gtacatgatt gtctgtcact gctttaagat tcttccagct
     9901 tggttcattg aacatggcag catctttgtc actgttcaca aggatgatgt agtcagcatc
     9961 taattcagct agcttttcag aggaaacagc agaccagtca gaagtggctt ttttggagac
    10021 ctcttccact agatcaggaa caccaaattc cagatcgtcg tatacgattc ttccacttga
    10081 ccgatttttg ctgaccataa aggctgagtt gttggtcacc cacaacactg cagctgtctt
    10141 gttctcgatt ttatctgcta gttcttctcg agttttcttg atggattttt gataatcgga
    10201 aatgattttt tctgcctctt tttcttttgc caaagctttg ccgacatctt ttagttgttc
    10261 ttcccatgtg acatctgttc cgttttttac aacataagtt ggcgcgattt tgctatattg
    10321 ttcgtatttc ccgccttcga cagtagcgga agaagaaatc aacagcaaat caggctcaaa
    10381 gcttaacact ttctcataag gaaggtcata agaaatcgtt gggacatcct ttaaatcatc
    10441 ttgcagataa tcttgaatgg aaccactgcc aacagtccac tgggcgatag gtttttcccc
    10501 tagtgcaatc agatagtctt ctaagtaact gccgatgatc cgtttgggcg cgtttggaat
    10561 ctctacttta tgtccaagtg tatctgtgac tgtatgagta ctattggatg aagtcgtcga
    10621 attggcagtt gtagctgttt ctttatctga agagggggag caagcgctca gtactccagc
    10681 cagcagcaat atgctagata atgtgaatac ttttgttttt aatttcattt ctttcctcct
    10741 aacataattg agaatctttc tcattggtta ttattcctga tatttcgaac gctgtcaacc
    10801 gcttcatttg gctatagata tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa
    10861 ttgagaatga ttttcagcga aaggatgggt gaaatgacga gattaagagc agctgacatt
    10921 gcagccggct acgaaaacaa aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg
    10981 aaaatcacta gtttgattgg gccaaatgga agtggaaaat ctactttatt gaaaagtttt
    11041 acccgtatac tacctgtgaa atcaggaaag atcattgtca ataactctga tatctctaca
    11101 tacagttcaa aagaacttgc aaaaaaaatt acattattag cacaaactag tgaacaccct
    11161 acaggtatga cagtagaaga agtagtatct tacggacgat acccttatca aaaactgttc
    11221 agtggcatgg acacgcaaga tcatgaagca atcgagtggg cgttggatgc tacaggtcta
    11281 gctggatttc gtatgcggga tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg
    11341 atcgctatgg cgcttgctca ggaaacagat atcttgatct tggatgaacc gacaacattt
    11401 ttagatccgg cacaccaatt agagatccta cagttattga ctgaaatcaa tcaaatgaaa
    11461 caaacaacga tcttgatgtc gattcatgac attaatcatg cttcacgttt ttcagattat
    11521 ttattagcaa tgaaaaacgg taagttgatc acagaaggta cacctgaaga agtgatcaca
    11581 aaagagtgta tgaaaaaaat atttgagatt gatgcaacga ttgtcacgct tccaagaaca
    11641 aataaaccag tttttctaac ctatgatttg ttgccaaaag agcaggagga tcaattatga
    11701 ggactatgaa gaaaaataga ctcctgcttt tggtctgttt tgttcttttg attatttttt
    11761 ttgttgctct ttcaaatggc gcagcgactg tgggaaaagc agatttttgg caggcgttgt
    11821 ttgattttga tagatcaaaa caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg
    11881 tggcgggagc atttcttgta gggagttgtt ttgcattgag cggtgcattg atgcaaggag
    11941 tgacaagaaa tcctttagca gattcaggct tactaggaat caacgcgggt gcttcgttgg
    12001 caatggccct tagttttgct ttttggccta aagcttctga tttttctgtg tttctaatgt
    12061 cattggcagg ttcttttgct gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa
    12121 tgggcatgaa tcctttacag ctgattttag ctggcgtagc tattagttct ttttttactg
    12181 ctatcagcca agcacttacc caattcttta atctgcagca ggatcttgct ttttggtttg
    12241 taggcggagc tgccaatatc acatggaaac aattagcagt attgtttcct ttatatatac
    12301 ttgctgtttt ggtttctttt tcattaggaa aagcaatatc tttgattcat ttgagtgaaa
    12361 cacatgcatt agctcttgga agtcatccta aacgaacgcg gttgattgtt ttctgtctag
    12421 ttgcagtgtt agcagccatt gccgtttcgt tagtcggacc tgtttcattt attgggctgt
    12481 tggttccgcc agttgttcgc ggaatggttg gaacggacta tcgttatgtt ctacctgtct
    12541 cttttttagt tggcggtgga ttggttatgt tggcggattt tacagcgagg atgatcaatc
    12601 ccccttttga aacacctttt ggattagtca ttgcattgat tggcgttccg tttttactga
    12661 tacaagtaag gagggaacaa cggtgaaaaa aatatattgg acgttctatt tcttgctttt
    12721 tgtacttgtc atcctgtcta taggtagttt aatgattggt acgcctttta tttacctgga
    12781 tcagcttttt gccacgttca tagggaaaga atcatcttct ttggcgctga ttgttagaga
    12841 atttcgagtt cctcgattac tgatcagttt attagcagga gcttgtcttg gcgtttctgg
    12901 ttatatcttg caaggagtaa caagaaatga attggcagat tctagtatat tgggcattaa
    12961 tgccggagca ggatttttag tgatgcttta tttaggtttc ttttcgcact attcgctgcc
    13021 gtttctattg ctagttgtag cctttctagg tggtgtttta gctgcttgct gtgtctatct
    13081 tgcggcctat gatcgaaatg gctttttggg tatgaatcga atcttattgt ccggtattgc
    13141 cgttaatgcc ggcctttctg cattaacatt gctatgcacg atccaattat ctaaggaaaa
    13201 ttatggattt gtgaatgcat ggttagcggg atcaatctgg ggtgcgagct ggccttatgt
    13261 ttgggcattg cttccatggg cggttatctt aattccgcta ggaggacttt atgcacagag
    13321 gattggattg ctatcatttg gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa
    13381 aagtcaaaaa atccttttgt tgttagctgt tgctttggca agcggtagtg tagcggtgac
    13441 aggtagttta tcttttgtag gactactagc tcctcatgct gcgaagctag tcgtacgtaa
    13501 agaaaataac tggacctttg ttctgagcgg tttgttcggt tccatttttc tgcttagtgc
    13561 agatatggtt gctagagtga ttctaccaag tggggaagta cctacaggaa ttttgattgc
    13621 cctttttggt gcgccatatt ttctgtatgc actttttatc agacaaaaac acgcgctgaa
    13681 cagttgaaat ttattaaaaa aggaaggcca atcatttcta ggtcttcttt ttattctatt
    13741 tatgcattta tttgaaagtt cgattgctat ttttcttttt ttcctatacc attttatgag
    13801 agaaggaagg gtgagttttt ggaccaattg aaagtaagat tacgccggaa aggaatcgaa
    13861 aagctgaaaa atctggccaa aagtggcaag aaaaaggaaa aagaacagcg aatctataaa
    13921 cagctattcg ccagcgagta ctggaagtca gcaaaagtga tagggacgac tttatcgaat
    13981 gaatttgaac tggatacaca gccggttatc caacgagcag aaaaagaaca aaagaaaata
    14041 gttgtaccta aaactttgcc gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc
    14101 gttcttgtac gttcgagttt tggtgttctt gagccacaat cagagaagct ctatttgccg
    14161 gagatgatcg atctcctcat tgtaccaggt attatctaca atagagccgg ttatcgaatc
    14221 ggttttggcg gtggttatta cgaccggtat ttaaataatt ttcataaaaa aacatgtagc
    14281 cttgtttttc gtgaacaatt gaatgaacaa tggcaaccgg agagctttga tcagaagatc
    14341 caacaattgt ttattgacta aaatgaaatg ggaggttttc atgaattatc aacagcaaat
    14401 aaaattgcgt gcatggttaa gacgaccgtt atggacctat gcttttttag ggatacagac
    14461 agcggtcttc ctcatgatgg aattatttcc gcgacttgag atcccttatt atgccggaat
    14521 gtatggtcct tatcttgttc acttcaatga atggtggcgt ttagtgacac caatcttcat
    14581 ccattttgga ctgatgcatt tcgtaatgaa ctcactgata ctgtatttta tggggcagca
    14641 aatcgaggca atctatggcc attggcgttt tttcttgatt tatatgttca gcgggataat
    14701 gggaaataca gcaagctttg cttttaacga agcgaatgtt ctgtctggtg gtgcgagtac
    14761 ttcgatattc ggtttgttcg gtgcgttgtt tatcttaggg tttcatttta aacataaccc
    14821 agcgatccag cagctagtca gacattttct tttatttatt gtgttgacat ttgtttttgg
    14881 tcttctggat acctctgtag atgtttgggg acatgtcggt gggctaattg gcggattagt
    14941 gttagggaat gttttgggtc taccgaaaca acagacaagc tattcgatac accaaaggat
    15001 tctttctaca ctagtttttg tatttctttt tgtaatatgt attttactgg gtttaaaaaa
    15061 atatggttta cttgtataat atgaaggaag tgcttaaaga tggagagctt atatgatgta
    15121 cagcagctgt tcaaaaaatt tggcatcttt atctatgtgg gtgcacgcat ctatgacatt
    15181 gaattaatga ctatcgaatt gcgtaatctg tatgatagcc gtttgattga tcatgataca
    15241 tacatgacag cgtggcgcat attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa
    15301 ggaaatttgt aatggacaag aagattattg ggattgattt aggtggaaca acagcaaaat
    15361 ttgcaatttt aacaccagaa ggagagattc aacaaaaatg gagcatcgat accaatattt
    15421 tggatgatgg taagcatatc gttcctgaaa tcattgaatc aatcaaccat cgtttgaatt
    15481 tatataatat gaaagccgaa gactttatcg gaatcggcat gggtacccca ggaagcgtgg
    15541 acagcgaagc tggaacggtt attggtgcgt ataacttaaa ctggacagaa gttcaatttg
    15601 tgaaaaaact gatcgaggca ggaacaggaa tcaaattcgc tatcgataat gatgcgaatg
    15661 ttgctgcttt aggtgaacga tggaaaggtg ctggagaaaa cgatccagac gttgtattcg
    15721 ttactctagg aacaggtgtt ggcggtggta tcgttgctgg cggaaatttg attcacggcg
    15781 tgagcggagc aggcggagag atcggccata tcactgtgga tcctgaaggg ttcgaatgta
    15841 cttgcggtaa acgcggatgt ttagaaactg tgtctagcgc tactggtgtg gtacgtctag
    15901 gtcgttactt ggcagaagaa tacgcaggag attcaaaact gaaagcaatg ttagacaacg
    15961 gtgaagaagt aacaagtaaa gatatctttg aaatggctca agaagacgat ccgtttgcat
    16021 tgatggttgt tgatcgcgta tgcttctact taggtttagc ttgcgccaac ttggggaata
    16081 cattgaatcc gtcaagtatt gtgttaggtg gaggagtatc ggcagcagga gaatttttac
    16141 gtagccgtgt agagaaatat tttaaagaat ttactttccc gcaagtaaga gaatcaacaa
    16201 aaatcaaatt agcagagctt ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct
    16261 tgaaatttgc ttaaaaaaac ggaggcatta catggtttta tgggtaatta atatcatttt
    16321 gctattgatt gtgcttgcaa tcatcttttg ggaagtctat ctacgagtaa tggccaagcg
    16381 ttcagcaacg actttgacag aagaagaatt tcgagaagga atgagaaaag cacaagtaat
    16441 cgatgttcgc gaaaaagatg tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc
    16501 ttacactgtt ttgaaagatt cattaggatc tattcgaaaa gaccagcctg tctatattta
    16561 cgatcaaaag aaaagcttga gcatccgtac agcaaataaa ttacgtaaag caggttatca
    16621 agagatttat ttacttaagg gtggatatga cggatggtct ggaaaaatca aaagtaaaaa
    16681 agtataaaac agatgattgt atgacagctt taaaaaccga atcaatcatt agattttgac
    16741 caagatcaca tgaacggcgc aatggatagt tgtgccgttc atttttttta ttttgtgcta
    16801 ttatctacat atataagcta agcgaaaagg cagaaagaag gaaacaacta tgatcaaatt
    16861 aaaacgtgtt taccttgatg ctgaaaaaga ggatggctat agaatactcg tggataggtt
    16921 ctggccacga ggaatttcaa aagaaaaagc gcagttagat ctgtggttga aagaaattgc
    16981 acctagtaat gaagcacgca aaacttttga ccatgtacca gaacgatttg cttcatttgc
    17041 aaagaaatac cagcaagaat tgattttcga agaagaaaag aagcaagcta tttttaaact
    17101 cagtcaactt atgaaagaac atccagtcat tacttttgtt tatggtgcaa aaaatgaaca
    17161 tcagaaccat gctgttttat tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata
    17221 gggatcattg gtgccggacc tagaggatta agtatagcag aaaggattct ccataataat
    17281 caggacagac attctcttga actttttctt ttcgatccca atggtcctgg aggtcgagtt
    17341 tggcggttga atcagccacc ggagttatta atgaattctg tttctcaaca agtgacgctt
    17401 tttacagatg aaacattgtc cagtggagga ttggtaacac agggacccaa cttgtttcaa
    17461 tggagtaaag gacctgctac agattatatc gaacagacgg taaccaaaaa taagcaacta
    17521 attatagaag aaatcaatcg tctggaaaaa gatcagcaat ctactcgatg tttatacgga
    17581 ctctatcaac gctggttttt ctcaaagttg caagaagaat ttccagcttc tattcagttt
    17641 gtttattctt tagtaaaaaa agtcagcaaa agaaaagatg gttatctttt acagacagat
    17701 cagcaaagct attctatcga tcagcttgtt ttagcaagcg gtcactggga aaatgatgag
    17761 acagaagaag aaagagtgtg ggtagactac gcaaagaaaa atgatttact gtatcagctt
    17821 ccttctaatc ctgcagatgt cgatctttcg aagataccgc ctaaagaaaa tgtcttttta
    17881 agaggtctag gcttaagttt ttttgattat gtcggattgt ttacagtatc aagagaaggg
    17941 cgcttcgaga acgaaaaagg aaaacttgtc tatcatccat caggcaagga accgattgta
    18001 tacagtggat caagaagggg gctcccttat taccctcgag gaagaaacca aaagcaaggc
    18061 ggatcaatgg catggccacg gttactaaca aaagaaaacc tggagcaatg gcatcagtcg
    18121 ggatttttga ctggcgaaac attttttgac tatctgaaaa aagacgttga gttgttttat
    18181 tacaagaaaa tcatcgaaga atatcatttg ccgattccaa agaaaaattt tgagcatgat
    18241 tttctctccc tctcttcaga ggagtgtctc ggaaagcatc cagcactaat cccttataga
    18301 tgggattggt cgtatttaga aattcctctt acctatcagg atgagtcctt tgcagaagct
    18361 agccgagcat ttattgaagg gcagataaaa gaagcggaaa aaggcaattg cacaggagca
    18421 tttgcttcaa catttgatgc attaaaagac tggcgtgatc caatccggca ggctattgaa
    18481 tggcacgttt tttctgcaaa ggaatacaaa gaaatactat ggggatggtt tactcatcta
    18541 aatagctttt taacgatcgg tccgccagtc attcggacga aagaattagc agcactgata
    18601 gatgcgggta tttttcattt gttcgaaccg ccatttgata ttcagcaaaa gcacaaccgc
    18661 tatgttattt ctctacaaga taaagaaatc agcagtcgct atttgattga agctcgattg
    18721 ccacaaacaa gcttttctcg ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa
    18781 cttgttcgcc ctttttcatt ttcagataat acaggaagtt atcattcaga agctatagat
    18841 gttgagcgag gtacgaatcg cgtgattgat gcttcagggg aaatccagtc tgacttttat
    18901 tgctatggaa tacctgttga aggtgtggat tggctaactg cagcagtagc tagaccttat
    18961 acagatccat ggaatctgag acaggcagat aaaatagcac aacttatatt gactgacaaa
    19021 taaaaatacg catgatgact tatctgttct ttcacaagtc ttcatgcgtg tttttaaata
    19081 attattaacg agaaacgcgt ttgcgcattg cttttttacg gttttcttcg atcattgctt
    19141 ctttttcgtc aattggatcg atcactgttt ttttcacaga agcgaccaaa ccagcaacgg
    19201 cagcggctgt tgccagactt cctactaata ttcca
//
LOCUS       DAYYKC010000057        17960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000057 DAYYKC010000000
VERSION     DAYYKC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.057"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHBBW_001872"
     CDS             complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHBBW_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9153541.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1699..1827)
                     /locus_tag="ACHBBW_001873"
     CDS             complement(1699..1827)
                     /locus_tag="ACHBBW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153542.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2172..2315)
                     /locus_tag="ACHBBW_001874"
     CDS             complement(2172..2315)
                     /locus_tag="ACHBBW_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9153543.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="ACHBBW_001875"
     CDS             complement(2538..3017)
                     /locus_tag="ACHBBW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9153544.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="ACHBBW_001876"
     CDS             complement(3090..3374)
                     /locus_tag="ACHBBW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153545.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="ACHBBW_001877"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBBW_001877"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="ACHBBW_001878"
     CDS             complement(3718..5004)
                     /locus_tag="ACHBBW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9153546.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="ACHBBW_001879"
     CDS             5142..5813
                     /locus_tag="ACHBBW_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9153547.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="ACHBBW_001880"
     CDS             5779..6267
                     /locus_tag="ACHBBW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9153548.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="ACHBBW_001881"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="ACHBBW_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9153549.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="ACHBBW_001882"
     CDS             7228..7992
                     /locus_tag="ACHBBW_001882"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9153550.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="ACHBBW_001883"
     CDS             8225..10438
                     /locus_tag="ACHBBW_001883"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9153551.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /gene="dtd"
                     /locus_tag="ACHBBW_001884"
     CDS             10449..10895
                     /gene="dtd"
                     /locus_tag="ACHBBW_001884"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9153552.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="ACHBBW_001885"
     CDS             11066..12325
                     /locus_tag="ACHBBW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9153553.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="ACHBBW_001886"
     CDS             complement(12414..13361)
                     /locus_tag="ACHBBW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9153554.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /gene="hisS"
                     /locus_tag="ACHBBW_001887"
     CDS             13755..15047
                     /gene="hisS"
                     /locus_tag="ACHBBW_001887"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9153555.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="ACHBBW_001888"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="ACHBBW_001888"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9153556.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="ACHBBW_001889"
     CDS             16940..17815
                     /locus_tag="ACHBBW_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9153557.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5677 a         3342 c         3837 g         5104 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt
//
LOCUS       DAYYKC010000058        17602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000058 DAYYKC010000000
VERSION     DAYYKC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.058"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHBBW_001890"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHBBW_001890"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9153558.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHBBW_001891"
     CDS             complement(2262..2513)
                     /locus_tag="ACHBBW_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9153559.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHBBW_001892"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHBBW_001892"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9153560.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHBBW_001893"
     CDS             3463..3681
                     /locus_tag="ACHBBW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153561.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACHBBW_001894"
     CDS             complement(3970..4482)
                     /locus_tag="ACHBBW_001894"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9153562.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHBBW_001895"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHBBW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9153563.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACHBBW_001896"
     CDS             complement(6935..7378)
                     /locus_tag="ACHBBW_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9153564.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACHBBW_001897"
     CDS             complement(7379..8167)
                     /locus_tag="ACHBBW_001897"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9153565.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHBBW_001898"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHBBW_001898"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9153566.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACHBBW_001899"
     CDS             complement(9282..9869)
                     /locus_tag="ACHBBW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9153567.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHBBW_001900"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHBBW_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9153568.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACHBBW_001901"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACHBBW_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9153569.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="ACHBBW_001902"
     CDS             complement(12786..13721)
                     /locus_tag="ACHBBW_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153570.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13907..14866
                     /locus_tag="ACHBBW_001903"
     CDS             13907..14866
                     /locus_tag="ACHBBW_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9153571.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14952..16046)
                     /locus_tag="ACHBBW_001904"
     CDS             complement(14952..16046)
                     /locus_tag="ACHBBW_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9153572.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16235..16996
                     /locus_tag="ACHBBW_001905"
     CDS             16235..16996
                     /locus_tag="ACHBBW_001905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9153573.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="ACHBBW_001906"
     CDS             complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="ACHBBW_001906"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9153574.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5056 a         3834 c         3086 g         5626 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
    13861 tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca
    13921 tcttaccata gacgaactga caatgataga atcatattat cttcaacata ataagccggt
    13981 tgaaatcgct aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt
    14041 caagcaaggc aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg
    14101 tggtagaaaa gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac
    14161 tcttggttgg acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat
    14221 gagaacactt tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa
    14281 aggtaaaaga aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc
    14341 aatccatgat cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg
    14401 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
    14461 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
    14521 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
    14581 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
    14641 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
    14701 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
    14761 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
    14821 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta
    14881 taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa
    14941 taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag
    15001 tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt
    15061 ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca
    15121 atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg
    15181 tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta
    15241 tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact
    15301 ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt
    15361 gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact
    15421 tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc
    15481 caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt
    15541 ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga
    15601 taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca
    15661 acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc
    15721 atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc
    15781 ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg
    15841 atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct
    15901 gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac
    15961 ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga
    16021 taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt
    16081 atccacttac attatagcaa acacaaagta aatggtctac accttttctt tttcttttaa
    16141 aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg ttatcattaa
    16201 agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag
    16261 tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc
    16321 ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag
    16381 tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga
    16441 agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca
    16501 cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact
    16561 gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg
    16621 cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta
    16681 taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca
    16741 gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc
    16801 tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac
    16861 tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac
    16921 agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga
    16981 agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg
    17041 attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca
    17101 ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt
    17161 tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt
    17221 caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga
    17281 tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac
    17341 ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt
    17401 gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg
    17461 gttctaaagt atctacataa tcaatataac aaataggata acgattagga atcaattcca
    17521 ttacttcagt agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc
    17581 attttcatta ctttgactat ca
//
LOCUS       DAYYKC010000059        17444 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000059 DAYYKC010000000
VERSION     DAYYKC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.059"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..523)
                     /locus_tag="ACHBBW_001907"
     CDS             complement(182..523)
                     /locus_tag="ACHBBW_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153575.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(620..1072)
                     /locus_tag="ACHBBW_001908"
     CDS             complement(620..1072)
                     /locus_tag="ACHBBW_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153576.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(1079..1627)
                     /locus_tag="ACHBBW_001909"
     CDS             complement(1079..1627)
                     /locus_tag="ACHBBW_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM9153577.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1672..2004)
                     /locus_tag="ACHBBW_001910"
     CDS             complement(1672..2004)
                     /locus_tag="ACHBBW_001910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153578.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2018..2275)
                     /locus_tag="ACHBBW_001911"
     CDS             complement(2018..2275)
                     /locus_tag="ACHBBW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153579.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2470..3012)
                     /locus_tag="ACHBBW_001912"
     CDS             complement(2470..3012)
                     /locus_tag="ACHBBW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM9153580.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(3033..4124)
                     /locus_tag="ACHBBW_001913"
     CDS             complement(3033..4124)
                     /locus_tag="ACHBBW_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9153581.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4132..6342)
                     /locus_tag="ACHBBW_001914"
     CDS             complement(4132..6342)
                     /locus_tag="ACHBBW_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9153582.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6358..8886)
                     /locus_tag="ACHBBW_001915"
     CDS             complement(6358..8886)
                     /locus_tag="ACHBBW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9153583.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8938..9348)
                     /locus_tag="ACHBBW_001916"
     CDS             complement(8938..9348)
                     /locus_tag="ACHBBW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9153584.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(9361..9579)
                     /locus_tag="ACHBBW_001917"
     CDS             complement(9361..9579)
                     /locus_tag="ACHBBW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM9153585.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9576..10568)
                     /locus_tag="ACHBBW_001918"
     CDS             complement(9576..10568)
                     /locus_tag="ACHBBW_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9153586.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10647..11558)
                     /locus_tag="ACHBBW_001919"
     CDS             complement(10647..11558)
                     /locus_tag="ACHBBW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9153587.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11577..11921)
                     /locus_tag="ACHBBW_001920"
     CDS             complement(11577..11921)
                     /locus_tag="ACHBBW_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153588.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11941..12219)
                     /locus_tag="ACHBBW_001921"
     CDS             complement(11941..12219)
                     /locus_tag="ACHBBW_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153589.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(12578..13078)
                     /locus_tag="ACHBBW_001922"
     CDS             complement(12578..13078)
                     /locus_tag="ACHBBW_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153590.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(13079..13624)
                     /locus_tag="ACHBBW_001923"
     CDS             complement(13079..13624)
                     /locus_tag="ACHBBW_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153591.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13621..14877)
                     /locus_tag="ACHBBW_001924"
     CDS             complement(13621..14877)
                     /locus_tag="ACHBBW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9153592.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(15237..15548)
                     /locus_tag="ACHBBW_001925"
     CDS             complement(15237..15548)
                     /locus_tag="ACHBBW_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153593.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15571..17046)
                     /locus_tag="ACHBBW_001926"
     CDS             complement(15571..17046)
                     /locus_tag="ACHBBW_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM9153594.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
BASE COUNT         5387 a         3721 c         2450 g         5886 t
ORIGIN      
        1 ttaacttata agaaattgca atttacttct tcaaagtgaa aacgaatatg agtaaaataa
       61 aattattctt cttacagata attaaaaata gcgcgtaaca aaaatcggca ttcaatcaca
      121 taattaggat tgatgccgat tacttatatt ctacgtatag ttttagtatt cttatttttt
      181 atcaccagta accaattgag aacagcccta aagcagaata gtcttttatc tcaaaacgct
      241 taatttcagg cttaggaata tgaataatct tttgagtata ttctttctct tttaatctta
      301 ctgaagaaat accactgcct atctttttta caaatatttc atttggtgta cagactaata
      361 aaacaatatt tgtaatcaat ccttgcacta aagaattaaa aggaagacta tttaaaacag
      421 ggtgtgcata actataacat gtgaagtagt tattttgaac tttaactttt aaaaacttta
      481 aaaataatct tatttgttct tctaaacaat catctatact catataatca ctccactaat
      541 taatctcccc aaaaagacac agcattaaga gatataccaa cacaacagtg caaataactc
      601 tacatcttcc ttcgcaacct tatttcaata aaccataaaa attatttttt aatagaaact
      661 gaaaattaaa actagaaaaa gtagttttac cgaatgtaag agcatcatca ccatcaatat
      721 agaaatacat tcttttccag ttacatttaa tttttaaaca aaattctccc caaaaaggaa
      781 ctttttctag tgaaaacttg gtaatctctt tgtaattaat tcttgttaaa gaataattaa
      841 acacttcatt tttacttgtt attttttcat tgactataac tagttcatct ggtaaaaaaa
      901 ttaaccaaca aacatcgaca aacagtaaat gatataatcc atttccacct ccaagcatat
      961 tcaatactgg tgaacatgcc ttagaaacac gaatatagtt atggtttaaa tagatatttt
     1021 ctttaaacat tgcttgttta aaagccatat gctcttgttc taaacttttc ataaactttt
     1081 actctaatgc acagctagca gcagctgctg caattcctgc tacaatattt accttagcta
     1141 actctttagc tgcagctttt cctaattgtt tttctgcaat ttttaataat aaatccgcag
     1201 ctttttcaaa attaaaagct tttaatgctt caataaaatc tccctgcgta cctatagccc
     1261 aagccactcc tcctccagga agagaattaa tggctacaca tccagcatat cgtttccaat
     1321 caactttctc cattctagga acaataatat gttcataaaa gactcttgct gctcttcctt
     1381 gttcgttatc ttgactgatt cgttcacgaa tagcaccaaa atcctttata tggtatacac
     1441 catgttcgtc taaaaaacca actgtatcaa aataaaaagt taattcatct actaatttat
     1501 tctttatttc taaatcaact gtattcactt tttctgtggc atacacactt gttccagtac
     1561 taacaaaagt acttaaacct aataagaaaa cacctaaaac accaaatatt ttaattattc
     1621 ttttcataat ttctcctcca taaaaaataa gttacttttt aaatatcttg tttatgaatt
     1681 cattccccaa aggcctttta agattgatac agcagtccgt tcacttcttt tagacttgtc
     1741 atacaagtct tttaaatgat cccatatcag actagtttgt ctatctagct tcaatccatt
     1801 aaacgctaat gaacggttca ttttttctat tactttagat aaagctacag aaatttttat
     1861 accttcacaa cgcgcatctt ggatttcaat aatagtatca tcaattagtt tttgaatttc
     1921 ttgcctatca acatattcaa tcttagaaag agaatctttt aaagctacta atgattcaac
     1981 gatttctttt ttctcttcag acattttctt cctccttcta gtgacccgta aagccatgta
     2041 gagcagagtc cattgaacca gtaattacac tatctacaac gccattccaa tttactctac
     2101 attttccatt tttcagattt gtcggtccat acggatagcc tttttgacat tcaatgttcg
     2161 gattatacca gataggatta taccattcag ccgcagaagc agattcagtc gatacaaaca
     2221 atcccacacc aacaaatcca atacacatca cactatagcc aattagtttt ttcatctcgt
     2281 atccatctcc taaaaaatag aatagttctt atagttattc ataagacata ttttttccgt
     2341 ttttgtttta tcctttctgt cctataacaa cttttttcaa gtaaagaatg acatagtttt
     2401 tcttaaaaaa acagtctgtt tttttctatt tcttcggtgt ataataaata tgagtgttca
     2461 ttttttattt taatacgcac tccgtacata ataaatcttg atcgcatcaa tcttttgagc
     2521 ttcttgacct actttcaatt gaagtaatac aaattgagta accttattca ttgtttcttc
     2581 tccaaataac aaatattgat ccggattctc tatatcttgt gtcacctggt aaagtttccc
     2641 agttgttttc atttgtacat gcgggtctac atcaatacta ttgtctttaa tacaacgatc
     2701 tgtcatatag tccctcacag cttgattgcg tttataaaca tctgtgaaat tactccattt
     2761 tctagcaaaa tcttttaaga aattttttgt catttcatct atttgcattt gttgcgctgt
     2821 ctcgtcttct aatgaaccaa tttgactgtt actttcagta ctttgaagag ttccacttgt
     2881 ttcttttact gcttcgattg tttcttctgt cgttctcaat tgtgtttttt gtttttcaga
     2941 agaaaaccaa tgacaaccag taagacctat caaacataac caactaaata ctaaaattat
     3001 cccttttttc acgtttctag tcactgcctt tcttatttca ccttacgaac cagactacga
     3061 atttttgttt gattgtacgt gcgttgatac cgagccataa tccgtccttg ctcggcattc
     3121 tgttccacac tagagaaatg ttgtccgcca tatgaaacat ctgttataat tccggtgtga
     3181 ccatagattc caggagacaa tgcaccacct gcttgccaat tcaccacatc tcctgcttgt
     3241 aaatcacttg gttttgggtt catgattact tgccaaccat aggttgtcca gttataatct
     3301 tcccctatta acatcgcaaa catgtgtcca gaacccatta actgtgggcc acctaattga
     3361 gatacatacc atgccgtacc accataacat tccccattat ttacggatcg tccaatgatc
     3421 cgatccaaaa ccttcgcatt gcctttactt tttcctgcaa taagcgaagt tttttttgtt
     3481 ggtaggctat tccaatgctt gatcgcaaaa gtatcatatt ccgttaagtt ataggacgtt
     3541 atgatactca ttgctcctcg ataatacgga ctcccttcgg taatacctgc tgccgcatag
     3601 cctccacgac ctagaatcaa taaacaactt ccaggatctt tattgcccac acattgatta
     3661 aagttgcttt ggctcgccac cataaagcca taatagtcat aacaagcttt taagttcgga
     3721 aacttcatat agaaaccacc ttcagaacca ccaattccac gtgcagaacc ttgtgggtag
     3781 ggacaaccag gaaaccaggt aatcccaaaa aagttcgtat cgtttcttcc agattgtgac
     3841 tgtccataag ctgattcata gcctaattga gaaagaatca cactcggtaa taactgatac
     3901 tttacagctg caccaactgc tgcttgcgca tgttcatctg tgattggacc acgtccttta
     3961 atctctgaaa aaatcggtaa tccttcggta tattccccat taaaagcgac agctagacct
     4021 gtagcttctc ctccatgctc agaaagttct cctgaaaaaa agaatggcaa tagaaaaaag
     4081 ggaagaataa agaaaagaag acaacctaga caccctttct tcatttcttc ctcatccttt
     4141 cttcgataat ggtttttgga ctgttcgtcc ttttggtaaa gtaaaatcag ttcctggttt
     4201 tgcgtccaaa atcggcagtg ttttaggatc cttttctatt ttttccgatt gctctgttgg
     4261 cttatctaaa gaagaaaatg caggttgtaa cgattttggt gcggaaatcg acggtttact
     4321 ttcagacgaa cgttgccccg gttgttgcac agaacgattt cctgatagag tggctaccga
     4381 cgaattagga acagagtatt ggttcaataa ctgctgcatt gctggataac tcccattttt
     4441 cgcctgattt ttcacttggc gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg
     4501 cacgggtact tttccagaag gcaacggaat cgattgacca acaaaagcga tacctttatt
     4561 taacaatggt tttctttgat tattcccttg ttgcttactc aattgtgcca atgattgtac
     4621 ctgttgtttt ggtcttactg aattcatgga cgaatttgga atcaaaccat ttgaaagacc
     4681 agaattccac tctgttttcg atttggtaga agacatttca ggctctaccc catgataacg
     4741 aacatgacga ccactataag gcaaggcttg atttgaacgc ccacctagtt caaagggatt
     4801 aaccacttgc ctccctttta ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa
     4861 tccatgtatc gctgcagaag cgagtggatt cgttgctcga tacacattcc ctgctaatcc
     4921 tgcacctttg attgccaaat tttgtccagc agttgatagt tgaccaggca gttgtttcat
     4981 ttgagacaaa gaatgacttg ctatgttttt cgtgttgttt atagtttgat ctgcagctcc
     5041 taaccctagt ttctttaata aatctttccg ttttacaatg agtaaaattg ataggagtgc
     5101 tttgacaaac atatttccta aaaaaccaaa aacagtattc attggaatta aatcgtctac
     5161 tatgttatag atcaaaaatg ccaccaaaat aatcacactg taaaaagatt tttgaaacat
     5221 caagcccaaa aaggacttta aggtattcat gagcatataa tcaaatactg gaacaaaaga
     5281 agcgagtaat gaaaaaggaa tcacaagtaa taaacacaac accaacattt gaaagataaa
     5341 acgaatcatg ccaatcacta caagtggtaa tccaatactt agattaagca atggcaccat
     5401 agaaccaacg actgctttat aaacagcttt agttggttga atatagacac gataagcctt
     5461 ctttttccct ctatcttctc ctgcagcagt ggtcactttt gtctcaatat cagataattc
     5521 ttcacttgac gcttccttgg ctagaaattc ttgtggttca atctctgctt gatacaattc
     5581 ttctaaattt gttgtcccat aatttaacaa tatatacgga tcaacaacag ccgctttata
     5641 ataaagttgg cgcacttttt gaatggcttc cttttgtgtc gttggcacta aaacaccatt
     5701 gacatctttt gtcgccaatt catctagact atttgtagca gaaaaaataa agccttctac
     5761 ctccacggac aaatcattta accctttggt aaaagcttct cctttactag gtacgttact
     5821 accatagcca aaccaaacca ttgaaaaaat gacaacgaag agaaatcgaa taaacaccat
     5881 ttttgcgtaa tgtttccctc taaatatacg aagagtaaat agatataaca catacaagac
     5941 gacaatcccc gcaccaatag gatcgtatat ttttgaataa atagtatccg aaaaagtgaa
     6001 aaactggtga attaactcgt tcattgcagc tccttcatag agcttctcaa tgacaaaatc
     6061 aaggccttgc caaatcaatt tacttaaact aaagaacata ttcgtcatca tttttagaaa
     6121 atctgtgcca tttgccccaa ataacatttt gcctaatgaa aattcactat cagataaata
     6181 agaagaaaag gtatcaatat gaaatttccc aatctcatag atcgattgat ccacctgctt
     6241 ttctgccgca tacaccaatc caccaacaaa aagcagtccg aatccaatcg ttaaaaaaaa
     6301 caaaacgcac catttctttt ccttatgctt tatataagcc attgtttcac ctcatttcta
     6361 ttgcatgatt aacgtatcct gttcttctac ttgagagtga cttgtttttt ccgttgtttg
     6421 taatgcagcg gtcatttctt caaacaaact gtgtacattg agttttccta cacgtccata
     6481 gggatctaac atccaacact gtcctttaat catgtggctc atttcagcta agttcgcttc
     6541 atttacttcc aaacgtaaat gattcaaaat cttttctcgc tcactgtctt catcaaaagc
     6601 aaagacgaca ccaaaattcc ctttttcttt atctgaacct aagtcaccaa taaattgtgt
     6661 atctaacact aattgattca tctgggaacg ccccacacgt gccattgctg caataatact
     6721 acgtcctgcg ttcgaattat taaataccca tgcttctgta aaataaatcg ctgttttctt
     6781 actactatct cgttttccaa acatttcaca aaaacgacct aaactaatca tcaaacacaa
     6841 agactttcga tccgcatctg tatatagttc tggtcggaca ttgtcatttg gtaatttcaa
     6901 atcttggatc tctaaaatcg ttgttttggc attaaagtct aatcccgggt tttctccata
     6961 agaaaatcct aaacgtaaaa tcgaatcttc taccgtcaag cgtaaaaact caccatattc
     7021 tcgaacctct ttttgttcca tttgttctaa atagtcgacc acatgcatca agccaacttg
     7081 ttgtccggct tctttttgaa ctaaaacttc ttggattccc tttaataccg ccccttttaa
     7141 aaggtgattg gtacctaacg gacttaactc atcaatcata tcctgggcta cttgtttcgc
     7201 atctactcgg gataaacaaa caattggatc taatacacca taattacggg gatttgtagc
     7261 atctaacgtg acataactca ttttttgcaa atgttctaca aacaaagggt aatgtttctg
     7321 ataatacggg tcttgaatga gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac
     7381 aaataaactt tgtacattca taaacgaact atacaaaaag agcaagccaa ctaaaaaggt
     7441 cttcccttta ccggtttttc cagttactgc tacatgaggc gcatcaaaca acgcaccttt
     7501 gattccttcc gcaatggcta gaagattcaa aaagacaaaa cgatttgacg cataaattaa
     7561 ttcttctaat ggtgtttttt ctcctcgaga accaccatcc cctaatgtat cttgccgacc
     7621 aatataccag ccggattcca tacctaattc tgatactgct gaaaacaacc attcgccaac
     7681 tgcttctaca gttgttcggt gcttccaatg taactgccgt cccaatggtt gccctaataa
     7741 acatttatag aacaattctc gttgatcatt ttttgcacgg tggatatgga taatcgtatg
     7801 aagttgttga ataaccgttg cactattttg acgaacttct tcaaccgttt gaccgtatac
     7861 aataaaacat gcgatccaat cgacgacagg ttgcccatta tctactcgtt gctttaaatg
     7921 ttttactaat cctcgaacag ttaataagcg ttcactaggt tcttcatcat tttgaaccaa
     7981 ttccttatgc tctgttttga accgttccga tagccaattc agttttaaac caatacctcc
     8041 ctgtcctttc aagcgtggat agtgaatttt cattcgcaat tctgctggga aagaaagatc
     8101 ctgagcaaat tgaaatagat cacaatttct catattttca ggtgtttccc caataggcaa
     8161 caatgctaaa taactactcc ctaactcatc ttctaattga agaaggcctt tatgttcctt
     8221 cggtatacgt acatacgttc cttgattgtc tttcagtact tcccaattat tttgttcttc
     8281 tactttgtga gccattcctc gaatatattg cagacgattg agatacaaca gctctttttt
     8341 tactaaggga cgaatagaaa agctataaag taattgtgtc acttctttta cataaggatc
     8401 actttcagaa aacaaagttt ccaaatcatc tacttctaat ccaaataagc gtgctaaact
     8461 tcctgctaaa tctaaccaga ttagatttat tacatcatga aaactctcca ccgttgcttt
     8521 ttctttgact ttgattccta aaataaatga aggacgatag acttgacctt ctcgttccaa
     8581 caatgtcact gtttcttttg cataatacgc accaattgct tgactatcgg gagaaaatcc
     8641 ctttcctaat gtgtcaaact tcgtccgtaa attcattgtt cgtggataca tcaccaactc
     8701 cacatcttca taatctgcta aaagcaacag taaacgagcc atcatttctt ttatttcctg
     8761 tgtctttttt ggtgtgtttt ttgaaattgt tcgtggaaaa acatgatagt aggcccagat
     8821 acaatcctct ccatttaaca aacaattttt cttgaatgct tttactggtt cttttacaag
     8881 caccaatgaa tttcacctcc caataaaaaa ggaacaacca gttgattgtc cctttttcta
     8941 atcctctaaa taaacatact tattcacaaa aagtaattct tccaatgcat tgtttgatac
     9001 cttgtcccag ttataataca cacaatcacc attcatcata cgaatcatat aacaaaacca
     9061 atcccataaa taaaaatgca ctttttttcc atcaggtttt agttttgcca gtgctaacac
     9121 acataaatag gggagtccta tataaaaaac aactcctgct ggtggaaaaa aagaaatgac
     9181 ccaaccaata aatgaacgta aaaataaata ttcaaagatt acgaccaaca aaaaagttgc
     9241 tgcattgact aatgcaactg gaaatggaag acgccaattg cttgtaatct gttgtacgcg
     9301 ataaggttcc gcataaacaa tcgaataatc ataaggttcc caatccactt cactatcctc
     9361 ctaatccata aataattttt caatgatttt accaaacgca tcaaatacac gtgttggatc
     9421 atttaagaag aaataaactg cggcacctgc tgcgatagat attaatgcat ttcctacttt
     9481 tgatttcata aaatttcgca atcccagata aacaaccacc attattaaaa aatagccacc
     9541 ttgcgttaat ataaaatcta caattccctt cagtgtcatt tccgttcttc tcctttccta
     9601 tccaaccaat tatgtgttaa tttttcaaca taatactgat ttgttttttt cgtaatgaca
     9661 atcgtcattt gttctttttg taaaagtgac gtatcaccat ctcgaaagac gaccgttgct
     9721 tttacaataa aaggtttttt cgtttcttta taacgcaata ctttcatctc tacttgatca
     9781 actttagctg tttgattgag tccttcaggt tctttcatca tgtatgacat atcttttgca
     9841 ttgtttttcg catacttatc taagaaatct tccacaaatc cttcaacatc tttcgcttcc
     9901 gacgcatcaa ccgtttcgta taatgtgtca tcataagtaa tcattcgttg tttttctgta
     9961 ttctttggta gtgccgtaaa ataaggataa gcaataatac gacagccctt gtttgaaata
    10021 gaaaagggaa gattgagtac ttgtgtgatt gtcacacttg tttctttctt actctctgga
    10081 ttcatcgagc gaatatcata agttacttta taactagcag ttggaatggt ttcaatttct
    10141 tttaatccaa taaacgaagc agcagttaat tttcgaacac ctgaaacact tcctggttct
    10201 tgaataaagt tttgtgcata aaactctttt tttaatgttt cttgacgttg ttgttcttgt
    10261 tcatgattat cgaatgtatt catatacaaa gcaacaaaac gattcataaa ggattcaatt
    10321 tcaggtgtgt acgttaattc tgaccgactt tgtacttctt tcttttgtaa ttgttggatt
    10381 tcatctttca aaaacgacac ttgaaccttc gttttcatgg attgaaaaaa tgccaagccc
    10441 cctgaaaaac aaagaaataa aaccaaacac caaatccatt tcactgcttt ttttgaatgg
    10501 attttctttt gcttcggtac tttctctgta taaacttttt ctttccgccg tagttgtcgt
    10561 tttttcataa gctactcctt ctagtatgtt tttactagta cgtacgattg acaacgataa
    10621 gcttgttttt ctgaaaaata gtccattcaa ttaccctcat tttctattcc ttttctcttt
    10681 cttttatatc caacaaatcc agcaatactt gtcaacactc ctcctagaac agaaagtgca
    10741 acatgtattt cttcaccagt ctttggtaag acttttgttt ttccatctgc tgttttgatt
    10801 tcaatattgc cacttggaag tttctttaca ggaatcgaca aatcctttac caaattccct
    10861 tcactatctt ggattaatgg aataccatct tttacaccaa caattgtttg atcacctgct
    10921 acttttaatg gggtggttgg tgttggaata tgggtcaatt cctctacatt actagtgaca
    10981 atcggtacac tcattccttg agaagtattc gtcgtaattt caccgcttgg ggttaccgat
    11041 actgtgactt cttttgctga aacaataggt ttattttctt ttggaattgc ttctgatttt
    11101 tcaacaggtt gtttcggttc ttctttttgt tcttcactgg tagacgacat cgttggctgt
    11161 tccgtacttg acgaatcagt attcttttca gaagaactgg ttgttcctga ttgttccgga
    11221 gttgtgacta tacttgaatc cgttgttgtt gaactatgca caccttcttc ttcgatcgaa
    11281 tcagtcggta aagttgattc aggaaactct ggttctggtg tcactggctc tttttcaggt
    11341 ttctctgact gcaattgttc aggttctgct ggtttttccg ttgttcctcc atttagctct
    11401 gtctcaacat ctggtaatcc ttcccctggc aatgtttcta caatttctga cttaggtgtt
    11461 tctacagttt catctgcata aaccaatgaa ctccctaatt gaaacgaaaa taatgaaatt
    11521 cctagtgttg ttacgactgc taatgttgtt tttttcatga ttattcctcc aattttttat
    11581 gcaattcgtg ttttgttcat agacgccgca ctaatattat ctgaacgata aaagaactcc
    11641 cgtccgtttt cttctaaccc aacatatgga ttatttttga ataattgaat caatagttca
    11701 ctggtgttgt attttggata gtttgcaaaa aaataatact gtgcttgtcg cccatccgaa
    11761 aattcaacaa gtaattcata acgataccgc ttcaatcctt ttcgttgttt taaaggatct
    11821 ctataccaac ttgtccatcg ttccattcgt ggatagagat agacacgata cacatagaac
    11881 aatgccatac cacataccac caaaaataat aatcgccaca taaaaattcc cccttttctt
    11941 ctacctttct tgaatcatcg aacattcata ccctctatag agatttgaaa aatccatcat
    12001 caattgttca tataaacgtt gttgcaaata gcgattgttt cgaatacata attcttcata
    12061 gccatcaatc acttgatctt ctttttcata gcatcttgta gctctatgac gctcagcttg
    12121 caagacataa ccaaactctg caccatatcg ttctactgtt aataactgtt gactacgcaa
    12181 taatgcaata taacaatcac aaaaatgttc aaccgacatt tgatttttct cacgtttgtg
    12241 aaataggtat tctttagaaa gcacattatt cgttaaacaa tctgataaat agcgataatc
    12301 tggcggtcgg ttattttcat ctgtgaattt ttcccataat aaactgattt ttattcgttc
    12361 agcggctaga tagtcttgtt cgcgtggata aaattcatca atcgcacaaa gttttttatc
    12421 acctgaataa acaaatccat ataatgcgaa acgaactctt ccttttcaat tggctctcct
    12481 aaaaattcca gtgacatacc atcttctcct tcctaaaaaa aataaaaaag cagaaaccga
    12541 tttggcttct acttttcttt ccttttatag ctctttttta cggaagaatc cgcaaatgac
    12601 attttttcaa aaaatcaatc ggcacaagtt cacgaaaact tggagcaatt ttggatagaa
    12661 tcacttcccc atcttgtaaa atacaatcat aaaaatcatc agttacttga tacttacgct
    12721 tcttacaata attgataaaa tagtagcgat cattttctat ttccattcgt tgttctacta
    12781 agcgcatacg aacatcatca tagaaataag ccacaaatcg cccttcaagt tcatgttttt
    12841 caatgggata ctcttctggt aacaatctat tttttcgtcg tttatacatt gctggatacc
    12901 ttagaagaag ttcatctaaa cgtctgtcag tatatggtaa ctgacgaatc aacgattcta
    12961 atcgttcttt atacagatag aataattggc gcaagcaata gtgtaccgcc tctggatttt
    13021 ctaatactgt tggatcacgt gaccaatacg caccaacata aatcataatt tttttcattt
    13081 agacaatcac accagcaata ctaatgaccg atccacaata ggtagaacaa gtggcatagc
    13141 gaccaatttc ctctaattct atctcaatct cttctaaaga aagatatgtc tgttcttctg
    13201 ttggctggcg actcatttcc caagtgacag tcaccaatga aatttcttcc ataagaggtt
    13261 tttcgctgct aagtgctgcc acacagtttt catatatctt cattttatcg cagccttttt
    13321 taaccgctaa aatcgcaggt attggcaatg aaaatcgata aaagcgaatg aattctagcc
    13381 ttcctttcct tcggctaaat tccaactctt cgagttcttg ttcgtatccc ttacgaactt
    13441 ctaataagcg tgcaatttct tgccagttct ctttttcctg ataacgtcca gcttttaccg
    13501 cttgttcaat ataaggaact cgaaaacgaa aataagacaa atcaaccatc ttttcattta
    13561 atgcttcgat tgcagccttt aaatccaaca ggcgcagcca ttctgttgtc gtatatgcat
    13621 tcatgcaatt ttccctcctg acgttcccat ccattctaga tagttttgtt gctcgtcatt
    13681 tagttcggca ttatcaatcc cttcttgtac aaagtctctt cctaaaatac gatctgcttc
    13741 ttgtaacaac tttaatgtcg gagctacttg tttcaaaaac cagcgttctg aacgatctaa
    13801 tgagaatttt tctggcttta ctgtaaaatc caattcttcc atcgaacgaa ataattttgc
    13861 ccatcgttta tcaacaatcc gtgcatcact ttccagtcta tcccaaacaa taagttttgc
    13921 attaattaaa ccaacgccaa tctctcctaa acgtttcggt gaatcaccta aaataaatgc
    13981 ttctactgct tgtgttgctt tctcattggc taaccgtaac tcatatcgat tataaatacc
    14041 atatttcgtt aatgcttcta attctgaaat tttttctcgg ttcgcaattt caaaacgttt
    14101 ctcataaaaa ttaaaaaaga gaggactctt tcgcgatcca aaatacaaag ataatccttc
    14161 acttcgaaaa tagccatcat ctaaaaaaca accgccaata ttctggaatc tcttaaagtc
    14221 catttctaat aaacctgctt tttgtttttc tagtaattca aagagatcga aatgttcttc
    14281 cgtttccaat ccccaaagtt catctaacgc aatatcgaat cggctacatt gcacacgtgt
    14341 ataaccagct tccaactttc cttgattttt ccaaatggaa tatagccatg ttcgccaatc
    14401 tgtttgttgt tcacgcatga cttcctcata ttcccggcaa ccttgtcctg aaaattgcac
    14461 aagtacattt tctgttacag ttcgttttga atgccagaac acccagatat tcccacgaac
    14521 agaaagacag tcaaaaagat acaagcccgt tggacgtgtt tcaaattctg tcagatccat
    14581 tcgtaataca cgttgaatca ctgttttgta gtctgtccca aaaaaggaaa gacgcaaata
    14641 atcaatcatt gctcgaagat ctattccgct tttcatatac aatcgaaagc gttccttgat
    14701 tcgttgttca aaatcctctg ggatttttct tttcccttgt tccatctttc taatgtaatc
    14761 ttttgagcag cccatcaatt gcgcaaatct tgcttgtgat agcggctttt tatctccact
    14821 tatattatga attgcactta acttctttct gtagcttttt agctcaatcg gtgtcatttt
    14881 ctcgctcctt ttataaaata acaaaatcga tttttttaac cctttagaaa caccgatata
    14941 tcaactagtc cccctcgttt tttgggaaaa gtagtacaac tgctgacctt agataatcac
    15001 ttgatacaaa agggtttata aaactttttt ctatgtgtta tgttatttct gccccccata
    15061 ttatatatcg gggggcttta cacaaagagg ccgtgcgcaa gcgcacgacc tctttgtgag
    15121 ctgcacacgc tgctagcgag tgcgcattat aggcccatgc ttcgcagcct taatgcgcac
    15181 cgccaggcag agtgcggcta ccagactacg tctagagccc tcccgataga taataactac
    15241 aaaattaaat gatctaattt tgttatttca ctatattttt caggagaaaa aacgcgataa
    15301 attgacaaag ttaaatcctc ttctaaatct actcgataat gatacaatcg agtcaatcgt
    15361 tctatatctt ttttcgttag caatacttga ttaataactt ttcctgtaaa aacacgttct
    15421 ttgcgcaaaa agcggtaatc agttacttgt tcaatacgat tcaaccaatc ataaaacgtt
    15481 ctggaagaaa gagaaggatt aatcattcgt attgctgtag ctactctata ataacaactc
    15541 tctgccattt aacttcacct ccttagttga ttacaaaata tgcgcatcag aaaaacttgt
    15601 tccttttgga tcttttgcta aatacatttg gcgctctagt tgttcttgta acaactgatt
    15661 atcatctccc atcgtttgat cttgactatc ctcttttact gtaaaatcat agacaatcat
    15721 ttcagtaaat ttctcaaatt ctttgataaa gtgataatct ttgtaaacaa atggcgcaaa
    15781 aaactctaca ggtacatcaa atcctggaaa agcatatcca cgaccaggtt gatctttgtt
    15841 gtaaaatggc ttatttttaa aatcttctcc aaaagtcatt aaataaccaa tgggagacaa
    15901 tcgaccaaca gaaatacgga atgttaaatt atcccgaaca ccacttggaa aatattctgc
    15961 atctggtcgt tgcattgcta aaataatgta catccctgct tgacgaccct ttaatactaa
    16021 tggctgaatc aatttatcga ctctatttct cgcttccata tccaacgaac cataaaaagc
    16081 tgcccattca tcaaaaaaga taaaatgcgg tggtaaccca taataagcat aatttttccc
    16141 tggttcatat ttaggatctt tcatggtttt catataggca taccgttgat tcatttcttt
    16201 taatgcattt cgtaaacaaa taatcatttc ttcatttatt ttgtaaaaca catgtccttt
    16261 gaatacatcg acgtcaccta atgctgctaa atctgccgct tttccatcgc aaatatctac
    16321 tgtcccaaca cttaaacaag cttgaattaa agaatacaat aaataggttt tcccaccacc
    16381 gattgcacca gcaatcaaca tatttggtga ttgatcatat acccaatcca aagttttcat
    16441 taaatgaata cgtccatttt ctgctataca ttgatcaata ggaatccgac cagaatctga
    16501 aaggaataca taattgattc gggctttttc tcgttcttca cgaattaaat cacgcaaaaa
    16561 tacttcagat aacaaatgac ccatttttaa aaatcgttcg tgaaaacgcc caccatctaa
    16621 aggaaaagaa accactaatt cttgtttctt ttgataaaaa taaacttttg gtaatctcat
    16681 tttttccctt gtttgtttct cactaacttg tttctttgta taaacaaacc gattgtcaat
    16741 taacattcga acaagtaact gacgccaata aacagaggca aaatatcctt cttttagtcg
    16801 cgaccaatgc atcaacccta aaattaaccc actaaccagc aacaaataca acacaatact
    16861 tattccagga ataagataca tttgccaatt cgaaaacgta cttaaacgtg gataaaatac
    16921 tttccaacta aaccaaccta acagaatcaa aaatggaaac aaaaagaaac cataatagca
    16981 aaaccataaa taacgattga aatagcgaat tcgccgacca cgatacttga aaaaatgagc
    17041 tttcaattgt cacattcctc ctttctgttt ttacttttcc tgtgctaaaa acttttgaca
    17101 aaaagcaact aatacaggaa tatcacattt agtaaaaaaa cataatcgaa aaagatttaa
    17161 tcgattttta taagggtgtg cacacatatt acatattcgt actatacgaa tccaaatcac
    17221 ttcctgatga tcttgtttcc actggtacgg taatgaaaaa cgaacaagat cacgtaaaaa
    17281 catacactgc ccaataaccg cacaaatata tgcatttgtt tgtacaaaaa tcgttggatt
    17341 gtctccaaaa tactcaatca attgttggat attttgataa caagcacaat ctctcaaagt
    17401 atttgattgg ttttatctga attcaaaatc tccccttcct tcta
//
LOCUS       DAYYKC010000060        17357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000060 DAYYKC010000000
VERSION     DAYYKC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.060"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..488
                     /locus_tag="ACHBBW_001927"
     CDS             51..488
                     /locus_tag="ACHBBW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153595.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            583..1467
                     /locus_tag="ACHBBW_001928"
     CDS             583..1467
                     /locus_tag="ACHBBW_001928"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9153596.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1691..4858
                     /locus_tag="ACHBBW_001929"
     CDS             1691..4858
                     /locus_tag="ACHBBW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9153597.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4926..5423
                     /locus_tag="ACHBBW_001930"
     CDS             4926..5423
                     /locus_tag="ACHBBW_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9153598.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5442..5906
                     /locus_tag="ACHBBW_001931"
                     /pseudo
     CDS             5442..5906
                     /locus_tag="ACHBBW_001931"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5903..6661
                     /locus_tag="ACHBBW_001932"
     CDS             5903..6661
                     /locus_tag="ACHBBW_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9153599.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6747..7487)
                     /locus_tag="ACHBBW_001933"
     CDS             complement(6747..7487)
                     /locus_tag="ACHBBW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9153600.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7548..8801
                     /locus_tag="ACHBBW_001934"
     CDS             7548..8801
                     /locus_tag="ACHBBW_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9153601.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9123..9350
                     /locus_tag="ACHBBW_001935"
     CDS             9123..9350
                     /locus_tag="ACHBBW_001935"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9153602.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9473..9799
                     /locus_tag="ACHBBW_001936"
     CDS             9473..9799
                     /locus_tag="ACHBBW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9153603.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9867..10580
                     /locus_tag="ACHBBW_001937"
     CDS             9867..10580
                     /locus_tag="ACHBBW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9153604.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10890..11740
                     /gene="licT"
                     /locus_tag="ACHBBW_001938"
                     /pseudo
     CDS             10890..11740
                     /gene="licT"
                     /locus_tag="ACHBBW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11861..13726
                     /locus_tag="ACHBBW_001939"
     CDS             11861..13726
                     /locus_tag="ACHBBW_001939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9153605.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13740..15183
                     /locus_tag="ACHBBW_001940"
                     /pseudo
     CDS             13740..15183
                     /locus_tag="ACHBBW_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15291..15992
                     /locus_tag="ACHBBW_001941"
     CDS             15291..15992
                     /locus_tag="ACHBBW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9153606.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16118..16630
                     /locus_tag="ACHBBW_001942"
                     /pseudo
     CDS             16118..16630
                     /locus_tag="ACHBBW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17028..17237)
                     /locus_tag="ACHBBW_001943"
     CDS             complement(17028..17237)
                     /locus_tag="ACHBBW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9153607.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5799 a         2911 c         3726 g         4921 t
ORIGIN      
        1 aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag aagtagataa atggagtggt
       61 tttgcaaatt tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa
      121 ataaaaaggc aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg
      181 ttttggtgtt aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg
      241 acatattaac agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta
      301 taaggtctga taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt
      361 tttatctgcc acctaagatt tttactttat taggtataca atgcctatca ttttctttta
      421 tttcctatat cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca
      481 aaaaataaat gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc
      541 tatcgttata ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta
      601 gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca
      661 aaattggaat cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca
      721 aaaaacttta ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga
      781 gatggaacag tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc
      841 ggatttttcc cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa
      901 cctgaggaag cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa
      961 ataaatgacg actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat
     1021 gatgtagatg cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc
     1081 aaacagttag cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa
     1141 gaaattgaga gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca
     1201 ttacttccaa atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct
     1261 tctttgatcg atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg
     1321 aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa
     1381 ttgacgacga atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg
     1441 ccctcacatt tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga
     1501 acaccgcttg ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta
     1561 caaaaaagaa atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat
     1621 gaaagtacaa ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg
     1681 atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc
     1741 tctcttgatg gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc
     1801 agagaatcaa agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac
     1861 agaatctcaa agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac
     1921 ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc
     1981 agcggatggg tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga
     2041 atcagtaact aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa
     2101 acgagatatc gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta
     2161 tatcaatgag atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc
     2221 aggtggattg acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt
     2281 ttggggaagc agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta
     2341 tgatgggtcc aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt
     2401 ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc
     2461 ctatttttgt agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta
     2521 tctagggaaa ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc
     2581 gccaggaatc catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg
     2641 cgttagcgat gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag
     2701 tggttcggga agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg
     2761 cggaatcatc caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat
     2821 caccatcaat acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca
     2881 tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa
     2941 caatgggatg cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt
     3001 tatcgacatc gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca
     3061 aaagctgaca gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc
     3121 ttacgtgaac agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt
     3181 tctagaaagt ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat
     3241 acgcgcagat tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc
     3301 aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat
     3361 cagtggggat gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa
     3421 acggattgga agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc
     3481 ttctttaaag atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga
     3541 taactttgaa aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac
     3601 cgacttaggc aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac
     3661 aggtgccgga atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga
     3721 atccttaaga gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga
     3781 aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat
     3841 tacttacgca gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac
     3901 agatgaaaat gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt
     3961 cgaagttcaa gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc
     4021 tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt
     4081 cgatgtgatt cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg
     4141 cacgatcaca ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt
     4201 tttgtcagaa acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca
     4261 actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc
     4321 taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga
     4381 tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga
     4441 aggtggtctc tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg
     4501 tttgacacaa gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca
     4561 gcgagaaacg aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga
     4621 tgcaggattt tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt
     4681 gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta
     4741 ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat
     4801 agctggtaat tactcagcag agataacatg gacattagca gatgcgccga cagaataaag
     4861 atcaaggagg atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt
     4921 tacttatgat ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat
     4981 tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata
     5041 taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa
     5101 cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg
     5161 tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa
     5221 acttagcaaa aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac
     5281 tttcactgaa agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc
     5341 aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc
     5401 aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa
     5461 aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc
     5521 caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa
     5581 aagagcctta cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg
     5641 atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga
     5701 agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg
     5761 cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact
     5821 ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt
     5881 taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg
     5941 aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga
     6001 aggaacgatt tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc
     6061 tcctgttgat ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac
     6121 atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg
     6181 tacagcaaaa acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac
     6241 acaagtacag aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt
     6301 atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc
     6361 aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc
     6421 atctaccgtt tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga
     6481 tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa
     6541 tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga
     6601 caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta
     6661 aaatcatatc aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc
     6721 tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa
     6781 cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat
     6841 tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat
     6901 tttaaaagta aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta
     6961 ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata
     7021 ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata
     7081 aagattttat cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga
     7141 cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga
     7201 ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca
     7261 ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata
     7321 tcgataaaga aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga
     7381 gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc
     7441 cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta
     7501 ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag
     7561 tgacacttta tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga
     7621 aacaactgct agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac
     7681 ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt
     7741 attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca
     7801 ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg
     7861 gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta
     7921 tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg
     7981 gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga agtgttcact
     8041 tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt
     8101 tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc
     8161 ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt
     8221 ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg
     8281 aatttttaga aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac
     8341 agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag
     8401 cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga
     8461 ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc
     8521 gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt
     8581 ttagtatagc gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag
     8641 gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg
     8701 tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag
     8761 aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag
     8821 tttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa
     8881 gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga
     8941 cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag
     9001 tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg
     9061 aagagcaata tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa
     9121 aaatgctgac ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct
     9181 ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag
     9241 agattctagt agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa
     9301 gggaagaaga cacgctactg catacacatg gtggctacct aaattattga ttttctatga
     9361 catttatttt aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt
     9421 aaaaaaatgg tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag
     9481 tatggaaaaa gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa
     9541 agatttggct atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa
     9601 aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt
     9661 attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga
     9721 agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa
     9781 aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca
     9841 gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag
     9901 tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga
     9961 tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag
    10021 aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa
    10081 tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta
    10141 gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt
    10201 cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg
    10261 ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag
    10321 agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt
    10381 ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa
    10441 ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag
    10501 aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc
    10561 tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa
    10621 aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg
    10681 catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat
    10741 gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg
    10801 ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt
    10861 ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg
    10921 tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt
    10981 ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgtttaag
    11041 agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg cagaggagat
    11101 cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg aattttcgcc
    11161 taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag caaaagaaaa
    11221 tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga aagaatatca
    11281 agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac tttctgagga
    11341 tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg aagacatgca
    11401 agtgacgatg accgctacca caatggtaag agatatactg agtatcatca gtaaattctt
    11461 cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc atctgcagtt
    11521 ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat ttctctacgc
    11581 attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca atgagtatct
    11641 tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa cgattcatat
    11701 tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac tgtatggcag
    11761 gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt ttcaatctgg
    11821 gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc aagcagttgg
    11881 tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag tacattgtgc
    11941 tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa agttaaaaga
    12001 agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag tgattggcag
    12061 taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag tttcggagga
    12121 tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct ctagtatatt
    12181 tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct tatcattggc
    12241 aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt ttgctgcagc
    12301 agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta aaaagttcaa
    12361 agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag ctatcacagc
    12421 agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc tttcaccatc
    12481 tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa gtaaattaga
    12541 gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc cgcttgtcgt
    12601 tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga cggttgccgg
    12661 taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg tagcaggtct
    12721 aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg gattcgtacc
    12781 gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa tgcttcttcc
    12841 agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa aagatacgaa
    12901 acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca ctgaaccaac
    12961 tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta tttctggagg
    13021 tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca gtttggtcag
    13081 cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa atgtgacagt
    13141 agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat tgattttagg
    13201 atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg caggagaacc
    13261 gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac tgccattgag
    13321 tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg ctattgatcc
    13381 agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt ttccaactgg
    13441 acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata tcgggatgga
    13501 tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg atctcgtcaa
    13561 agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg gctatagcgt
    13621 aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg aattagacca
    13681 aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag gaaatcagga
    13741 tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc aaatcaatta
    13801 gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat gcctggaggt
    13861 aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga tcaagaaaaa
    13921 tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga agatcttgcc
    13981 ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac acgaattttt
    14041 ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga tcagctgatt
    14101 gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta cgaaatgcct
    14161 cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga cttctatgaa
    14221 cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg gatgactttc
    14281 aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt aaaaagcaat
    14341 ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt tgcaagtagc
    14401 aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg catgattatc
    14461 tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac aatgatccaa
    14521 aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata ccctgcatac
    14581 acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca aacggaaaaa
    14641 gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta ttatatgtct
    14701 acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt gctgggagga
    14761 gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc agaagggctg
    14821 agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat tgttgaaaac
    14881 ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga ttatcggatc
    14941 gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg tgtagatttg
    15001 atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg agaaatgagt
    15061 aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac actgaatcga
    15121 tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg cgtaaacttg
    15181 taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt gttctttttt
    15241 tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt atgaaaagga
    15301 cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc tcaggaaaat
    15361 atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt caagtcagca
    15421 aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc gcaaagaaaa
    15481 gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc aaagcggcaa
    15541 gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg ctggatttgc
    15601 aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag caagctgttt
    15661 gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt cattatcttc
    15721 cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg tatcgtttgc
    15781 tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca gtatttttaa
    15841 cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa cggatacgga
    15901 aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac catacggagc
    15961 tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg aaaaagctgc
    16021 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcgtttggtt
    16081 aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg gataaggggg
    16141 tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt tagcttcgat
    16201 ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc gtaaaaatat
    16261 actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga tctgtcagat
    16321 cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca tagcaacggg
    16381 agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata gccgtattcc
    16441 ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca ttggtgcatt
    16501 gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac cagtagatcc
    16561 gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg atgatttgac
    16621 aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg taccttgaga
    16681 caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt ttggtttagg
    16741 agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga aggaaaaatg
    16801 agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag gaaacaaaaa
    16861 aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa aaagaaatta
    16921 ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag ttccaggcag
    16981 gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca agtgatattt
    17041 ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg atgaacaaaa
    17101 atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac atcatttatc
    17161 agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac aagtatttgt
    17221 tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct tctataagta
    17281 agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata aaatggctga
    17341 aaaatgattt ttttcta
//
LOCUS       DAYYKC010000061        17338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000061 DAYYKC010000000
VERSION     DAYYKC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.061"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..1506
                     /locus_tag="ACHBBW_001944"
     CDS             79..1506
                     /locus_tag="ACHBBW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153608.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1598..1963)
                     /locus_tag="ACHBBW_001945"
     CDS             complement(1598..1963)
                     /locus_tag="ACHBBW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9153609.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1960..2325)
                     /locus_tag="ACHBBW_001946"
     CDS             complement(1960..2325)
                     /locus_tag="ACHBBW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9153610.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2517..2765)
                     /locus_tag="ACHBBW_001947"
     CDS             complement(2517..2765)
                     /locus_tag="ACHBBW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153611.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2764..2892
                     /locus_tag="ACHBBW_001948"
     CDS             2764..2892
                     /locus_tag="ACHBBW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9153612.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2949..4643
                     /locus_tag="ACHBBW_001949"
     CDS             2949..4643
                     /locus_tag="ACHBBW_001949"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9153613.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4710..4916
                     /locus_tag="ACHBBW_001950"
     CDS             4710..4916
                     /locus_tag="ACHBBW_001950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153614.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5294..6427
                     /locus_tag="ACHBBW_001951"
     CDS             5294..6427
                     /locus_tag="ACHBBW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9153615.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6458..6778
                     /locus_tag="ACHBBW_001952"
     CDS             6458..6778
                     /locus_tag="ACHBBW_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9153616.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6807..7271)
                     /locus_tag="ACHBBW_001953"
     CDS             complement(6807..7271)
                     /locus_tag="ACHBBW_001953"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153617.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7403..8656)
                     /locus_tag="ACHBBW_001954"
     CDS             complement(7403..8656)
                     /locus_tag="ACHBBW_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9153618.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8685..9002)
                     /locus_tag="ACHBBW_001955"
     CDS             complement(8685..9002)
                     /locus_tag="ACHBBW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9153619.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9028..9348)
                     /locus_tag="ACHBBW_001956"
     CDS             complement(9028..9348)
                     /locus_tag="ACHBBW_001956"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9153620.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9812..11227)
                     /locus_tag="ACHBBW_001957"
     CDS             complement(9812..11227)
                     /locus_tag="ACHBBW_001957"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9153621.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11244..12560)
                     /locus_tag="ACHBBW_001958"
     CDS             complement(11244..12560)
                     /locus_tag="ACHBBW_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9153622.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12710..13408)
                     /locus_tag="ACHBBW_001959"
     CDS             complement(12710..13408)
                     /locus_tag="ACHBBW_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9153623.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13697..14686)
                     /locus_tag="ACHBBW_001960"
     CDS             complement(13697..14686)
                     /locus_tag="ACHBBW_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9153624.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14766..15599)
                     /gene="msrA"
                     /locus_tag="ACHBBW_001961"
     CDS             complement(14766..15599)
                     /gene="msrA"
                     /locus_tag="ACHBBW_001961"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9153625.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15774..>17338)
                     /locus_tag="ACHBBW_001962"
     CDS             complement(15774..>17338)
                     /locus_tag="ACHBBW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9153626.1"
                     /translation="FKNTQSIYTTGDLSFHLSRIKGLSTIFTSPINYETFNYTGYGVN
                     YFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFS
                     CLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLL
                     VYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPM
                     FEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKK
                     LTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIF
                     FKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKG
                     MDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLP
                     VYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAY
                     LCLFFKIVFHKR"
BASE COUNT         5464 a         3449 c         2935 g         5490 t
ORIGIN      
        1 taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata
       61 caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg
      121 tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc
      181 ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa
      241 gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat
      301 atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt
      361 ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc
      421 tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg
      481 atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt
      541 aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa
      601 cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa
      661 aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct
      721 gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc
      781 gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt
      841 attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa
      901 gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt
      961 gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg
     1021 ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt
     1081 tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc
     1141 tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg
     1201 atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca
     1261 aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat
     1321 tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag
     1381 tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa
     1441 aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac
     1501 aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt
     1561 ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg
     1621 gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta
     1681 gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa
     1741 gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc
     1801 tgactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg
     1861 atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt
     1921 tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct
     1981 ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat
     2041 aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat
     2101 atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt
     2161 atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag
     2221 tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat
     2281 agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt
     2341 tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa
     2401 aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc
     2461 cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag
     2521 tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta
     2581 aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca
     2641 ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt
     2701 tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt
     2761 ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct
     2821 gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc
     2881 gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa gacattggag
     2941 gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag
     3001 cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat
     3061 tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga
     3121 ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta
     3181 attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc
     3241 cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca
     3301 aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata
     3361 ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac
     3421 tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact
     3481 tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc
     3541 gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca
     3601 atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag
     3661 aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac
     3721 cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag
     3781 aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag
     3841 attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag
     3901 cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat
     3961 tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt
     4021 tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat
     4081 ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg
     4141 tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt
     4201 tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa
     4261 aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata
     4321 ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc
     4381 cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta
     4441 aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct
     4501 ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc
     4561 ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg
     4621 ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa
     4681 taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc
     4741 agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag
     4801 taataagagc agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac
     4861 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc
     4921 gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa
     4981 taccgagaaa ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga
     5041 aaacaactat tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa
     5101 ctatttcaat gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata
     5161 cataagacta tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga
     5221 tctcctttaa aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa
     5281 aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa
     5341 cgtagatgat ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc
     5401 catttgcggc tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta
     5461 tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt
     5521 gaaaaaaggg caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg
     5581 cctgaatcac atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct
     5641 tttcggattt ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt
     5701 tccttatgca gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt
     5761 tttatttcta tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa
     5821 aaaaggcgat acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt
     5881 cgctgttatg gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga
     5941 taaagcggct aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg
     6001 aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat
     6061 ggacggatta gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac
     6121 aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac
     6181 tggtgtttat atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat
     6241 cgctgtcaag catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat
     6301 tgcagaagga ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc
     6361 agcacaagca tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa
     6421 accttgaatt cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac
     6481 ccatgagatc gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt
     6541 ctatttccaa aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt
     6601 ctatattttt ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt
     6661 ttatttggct ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat
     6721 ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga
     6781 atttttcaaa tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg
     6841 gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac
     6901 gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg
     6961 atttccgcta atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg
     7021 gcacaaagat ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct
     7081 cctccgacga tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta
     7141 ttagcgattc cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat
     7201 gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta
     7261 tatgtaacca atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc
     7321 tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac
     7381 ttgttctccg ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg
     7441 tagctacctt gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta
     7501 gtacaccacc tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc
     7561 atggaggcat aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac
     7621 taactaagac cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag
     7681 gtaacccaaa catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg
     7741 ttacaaccat atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta
     7801 aagttgcacc tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa
     7861 acggtgcgct atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga
     7921 ttgtttgcat caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg
     7981 gtgtgataaa tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt
     8041 cttggaccac tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg
     8101 cagcaactaa agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt
     8161 ttgcaactgc cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta
     8221 aacgtacaaa gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg
     8281 agctcatacc taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg
     8341 aaacaccgtc ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga
     8401 cagaaagaat ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac
     8461 caccaaaaat tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta
     8521 ctgctaatgc tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa
     8581 ctaaatgccg ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg
     8641 caacaaattt atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga
     8701 cgatacagat caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga
     8761 tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc
     8821 agcatctctg tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt
     8881 tcatctgcta aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt
     8941 tttgcatttc cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc
     9001 atttattgta tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag
     9061 tactttttca ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc
     9121 tttaggtgct agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag
     9181 taaaacgtct acatttttag attctagttg attatccgca tctgaagcag atacagcaaa
     9241 gatgtctgct tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact
     9301 tgtactcatt ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt
     9361 cctttctata cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa
     9421 aaagttgata tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag
     9481 cgcacactaa acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa
     9541 agttgtgaca caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat
     9601 ctgcggtaat aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct
     9661 tattacttga tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg
     9721 accctgggta ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta
     9781 aatacaatga acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca
     9841 attctctctt atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc
     9901 gttcatcgtt caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg
     9961 acacacagtc agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg
    10021 ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga
    10081 ttcctttctc atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca
    10141 gccaagggat attatgataa ttttctttta gatacatggc tatatcatac atgattttcg
    10201 gatagatttc ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat
    10261 aatatcgttc aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc
    10321 gtttaggata atattgattg attccaagga aatctactgt atgatgctga atcgtttcta
    10381 aatcctctgt tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt
    10441 ctgtgtagta tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca
    10501 tataagcagc tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta
    10561 aaatacagcc aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg
    10621 ttgctaaaac tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc
    10681 agcgaacagc atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc
    10741 agttttttac cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat
    10801 caactacttt tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat
    10861 aatgttccaa acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac
    10921 ttatatgctg agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag
    10981 cccaattgat agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct
    11041 gctggtattg ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa
    11101 aatgttcatt tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc
    11161 aaccttccgt ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa
    11221 ttttcatgat ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt
    11281 acaaatggca gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca
    11341 ccgatatttc ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc
    11401 attacaactg ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg
    11461 ataagaggcg ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca
    11521 aaaataactg gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt
    11581 ttcatatctt cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca
    11641 gacccgccca tactcatata catatcccaa aatggcatag taataatgtt cggtacttct
    11701 tttccagctt caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt
    11761 atcggcttca ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac
    11821 atcaatagca aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca
    11881 ttgtaaatga tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc
    11941 gcgatacccg atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc
    12001 gttggcacgg tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa
    12061 agttcaatag aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat
    12121 tgtttagcca atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca
    12181 gttggattaa cagataaata agacatcact gcgatgatac cagggaatac accatccact
    12241 ttattcagct tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt
    12301 agtgtgacat agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa
    12361 cttatctgtg ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa
    12421 gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac
    12481 cttaaattat tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa
    12541 ctcattccag atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt
    12601 agtcagcgct tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa
    12661 aacaaaaatt catctgatgt atttatcata aaatacatca gatgaactgt caattagttt
    12721 ctttttctcc tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg
    12781 catcaaacgc agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat
    12841 cgatcgtctc gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt
    12901 cattaatgat tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg
    12961 tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa
    13021 tcttttgcgt taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca
    13081 aacctgccat cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat
    13141 cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga
    13201 caaatgtccc cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag
    13261 cttctcgcac cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca
    13321 gtttatctcc tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta
    13381 cttctactag ggttttattt tcttccaaac cacatacctc cttttcatct gatgtatttt
    13441 ttcgtaatta ttataacacg tttgaatcat ctgatgattt tttgtataca aaattgatta
    13501 atccgttcat agacatacaa acgaagaaga tatttaccat aaaaaagact aggataaaat
    13561 tcactcctag ccttcgatca tttttatttt gaacgacaaa aaatatgatg ttcaataaca
    13621 gacgatcttc attcgattct tgatgtcatc ttttgttttt tttaatatat ggataaaaag
    13681 tttgattaca tcgtttctat tctagaattt ggtcgattct caattcttct tctggtgaga
    13741 aatctaaatt atccatcatt cgaacacttt cttgtaactg acttaaccga cttgctccga
    13801 ccagtacact tgtaacagat ttttgtcgca aattccatgc aagggccatt tgcgctaatg
    13861 attgtcctcg tgatacggcg attgtttgca gtgctttgat tttttccaga gttgatccaa
    13921 cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc tctagaatct tcaggaatac
    13981 catgtagata acgatttgtc agcaaccctt gagcaagcgg actgaacgtg attgccccta
    14041 gcccttcttc ttctaatact ggagatagat cgtcttctag tagtgcacga gaaaacatat
    14101 tgtagcgcat ctgatgaata atgaacggtg cttcctttct ttttaaaatt tcagtcattt
    14161 tttttgtatc ttctccatta tagttggata taccaatata taatgctttt ccttgtctta
    14221 caagttgcat caaagcttct gctgtttctt ccaaaggtgt atcagggtct gggcgatgat
    14281 gatagaagat gtcaacataa tctagcccca ttctttggag actttggtca caacttgaaa
    14341 tgatggattt acgagagccc cattccccat atggcccttc ccacatatga taacctgctt
    14401 tgctagaaat aatcagttca tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa
    14461 agtttttctc tgcagctcca gcagggcctc cataattatt cgcaaggtcg aaatgggtaa
    14521 ttcccatgtc gaatgcgcct cttaaaatct ctctttgatt atggatagga tcataatcac
    14581 caaaattttg ccataatccg agagataaaa tcggtaattt caaacctgaa tgaccggatt
    14641 tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt tctcctcctc
    14701 atcaaatctt tttaaaacgc tttctttttt ctatttttag tttactacat tttaaagaaa
    14761 aggagttaat caaaatgatt ttttcgtata tttttttgcc atttgttttt tactcgttga
    14821 tagatcagaa attgattacg tgcttgctgg atttttttat aacgtttcgg ttgttttttg
    14881 taaaattgct gatgatagtt ttctgctggc caaaacgtac cggctggttc gatttttgtg
    14941 acgattggtt ggtggaaagt tcctgaactg tctagtttct gttttgattg ttccgctaat
    15001 tctttttgtc gttcgacttg ataaaaaatc actggacgat attgcttccc tctatcttga
    15061 aattgtccaa atgtatctgt gggatcagta atttgccaat agattgtcag caactcgcta
    15121 taacttattt ctcttgtgtc ataaatgatc tccacagctt ccacatgacc tgtgtaacca
    15181 ccacttactt gatcataatt tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca
    15241 gataggatcc cttttttcga ttcaaacggc tcgaccatac accagaaaca tccccctgca
    15301 aatactgcac gttcttgata gtcagcatct tgcatggcat gcttggaaaa gtcatatttt
    15361 tgctccttat caaatccttc cgtaatcaat aaataaaagt ccattacatc aggcgtcaga
    15421 ttcattcgga tagctaatgg acgtagtgtt gcttctagtt ttgataactg ttctttaacg
    15481 gatatatttt ctcttatgtc attctttgtc tgaatcaata cttttcgttc ccaatctcgt
    15541 gtattcggat tcaaaatcag attataaagt gtttgaatga atatttgttc ctcatccata
    15601 gattctcctc ctttccgaag aagacaagct agtgacttta gctgtaattt atcataaagt
    15661 gaggaaataa gcgattactt catcccccta cagatttttc gtataaaaaa gagagaatcc
    15721 ttatcgacag attctccttt ttaatcaatt tattttataa atgaaaagtt aatttacctt
    15781 ttatggaaaa ctattttaaa aaataaacat aaatatgcag ttaatgtaac aagataagaa
    15841 ataatagtcg ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg
    15901 cttatttgta ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct
    15961 acattcgttc ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt
    16021 actctatata tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca
    16081 atctttgctt cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa
    16141 tcatataaaa aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca
    16201 acaatatgtc ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt
    16261 aaagtgatta ataacaaaac agtcataaat aaataaaata gttctcttgt cttattgtat
    16321 cttctatttt tgaaaaatat catcgttaat atcagagaag agaatattat ttgcaagctt
    16381 aagatccgcc aaggaaattg taacaatttg gctggcgtat tttgcagaag ataccatggc
    16441 accaaactag ttgtagctag ccaagtcaaa cacgtaccat aataaatctt tttttctatg
    16501 tttgttaatt ttttaaataa aatcagggga atacataaag ataacagacc aactagtccc
    16561 attgagtaag tagttaagtc gttgttcata gcttcagtca gactatcgaa cacattagat
    16621 gcatgagcat ataaatttgt cacgctaggt ttgttgattt tttggtagag catttgttca
    16681 aacatcggat accaaaaagc actcgtcaga accaatgtca ttcctgccgc actaaataat
    16741 tttttaaata tagcaataaa atctgcattc ttgaaacctt tagaaaccaa acgaataaaa
    16801 ataaaaatga taattaatgc tgtactcatt aatgttgata gtacatggct gtacactagc
    16861 aaactcatac tcaatgctag ctttacagac ggatactttt cttcttttcc agagatgatt
    16921 tgataagcat aaaataatac agggataaca agtgttattg cgatagcttc ggcaattgcg
    16981 gaacgatagt agatgtctac tgtccgataa gcagagaaaa tatagagaca actaaataaa
    17041 aatgccgctt ttttttgttt taaaaaccgc tcgccataat gataagctac gagtgttgta
    17101 cacaagttca ataaccaaac gtaaataata tagcttacaa tcaaattttt tgtcatccaa
    17161 tataacatta cagctggaaa aaaagtcaga aagggataaa aataatttac accatatcca
    17221 gtataattga atgtctcata attaatcgga ctggtaaaaa tagtcgataa ccccttgatt
    17281 cgagataagt gaaaagaaag atctccagtg gtataaatac tctgtgtatt tttgaaaa
//
LOCUS       DAYYKC010000062        16780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000062 DAYYKC010000000
VERSION     DAYYKC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.062"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..743)
                     /locus_tag="ACHBBW_001963"
     CDS             complement(30..743)
                     /locus_tag="ACHBBW_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9153627.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            918..2342
                     /locus_tag="ACHBBW_001964"
     CDS             918..2342
                     /locus_tag="ACHBBW_001964"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9153628.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2641..3348
                     /locus_tag="ACHBBW_001965"
     CDS             2641..3348
                     /locus_tag="ACHBBW_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9153629.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3335..3658
                     /locus_tag="ACHBBW_001966"
     CDS             3335..3658
                     /locus_tag="ACHBBW_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9153630.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3747..4784)
                     /locus_tag="ACHBBW_001967"
     CDS             complement(3747..4784)
                     /locus_tag="ACHBBW_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9153631.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5105..6088)
                     /locus_tag="ACHBBW_001968"
     CDS             complement(5105..6088)
                     /locus_tag="ACHBBW_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9153632.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6503..7273)
                     /locus_tag="ACHBBW_001969"
     CDS             complement(6503..7273)
                     /locus_tag="ACHBBW_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153633.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7266..8201)
                     /locus_tag="ACHBBW_001970"
     CDS             complement(7266..8201)
                     /locus_tag="ACHBBW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153634.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8517..10166)
                     /locus_tag="ACHBBW_001971"
     CDS             complement(8517..10166)
                     /locus_tag="ACHBBW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9153635.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10283..12886)
                     /locus_tag="ACHBBW_001972"
     CDS             complement(10283..12886)
                     /locus_tag="ACHBBW_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9153636.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12898..13947)
                     /locus_tag="ACHBBW_001973"
     CDS             complement(12898..13947)
                     /locus_tag="ACHBBW_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9153637.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13973..14287)
                     /locus_tag="ACHBBW_001974"
     CDS             complement(13973..14287)
                     /locus_tag="ACHBBW_001974"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM9153638.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14303..14749)
                     /locus_tag="ACHBBW_001975"
     CDS             complement(14303..14749)
                     /locus_tag="ACHBBW_001975"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9153639.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14750..16672)
                     /locus_tag="ACHBBW_001976"
     CDS             complement(14750..16672)
                     /locus_tag="ACHBBW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9153640.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4975 a         3411 c         2762 g         5632 t
ORIGIN      
        1 taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca
       61 aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca
      121 acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca
      181 aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt
      241 tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg
      301 actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt
      361 cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc
      421 gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg
      481 ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact
      541 tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct
      601 ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt
      661 ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc
      721 atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata
      781 ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata
      841 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
      901 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
      961 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
     1021 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
     1081 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
     1141 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
     1201 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
     1261 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
     1321 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
     1381 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
     1441 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
     1501 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
     1561 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
     1621 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
     1681 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
     1741 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
     1801 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
     1861 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
     1921 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
     1981 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
     2041 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
     2101 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
     2161 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
     2221 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
     2281 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
     2341 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
     2401 aataacaaat actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca
     2461 aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact
     2521 acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag
     2581 aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
     2641 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt
     2701 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg
     2761 gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc
     2821 atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc
     2881 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
     2941 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
     3001 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
     3061 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
     3121 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
     3181 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
     3241 attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca
     3301 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
     3361 gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact
     3421 gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
     3481 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
     3541 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
     3601 cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat
     3661 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
     3721 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
     3781 cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
     3841 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
     3901 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
     3961 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
     4021 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca
     4081 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
     4141 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
     4201 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
     4261 agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg
     4321 cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc
     4381 aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg
     4441 acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct
     4501 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact
     4561 ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga
     4621 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     4681 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac
     4741 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     4801 cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt
     4861 tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg
     4921 ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa
     4981 ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg
     5041 ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa
     5101 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc
     5161 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
     5221 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
     5281 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
     5341 tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca
     5401 tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat
     5461 ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag
     5521 ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
     5581 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
     5641 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa
     5701 tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta
     5761 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
     5821 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
     5881 gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga
     5941 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
     6001 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
     6061 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
     6121 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
     6181 tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca
     6241 caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac
     6301 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
     6361 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
     6421 acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa
     6481 cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc
     6541 aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg
     6601 gcacatcgat tgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat
     6661 tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac
     6721 ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact
     6781 gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg
     6841 taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg
     6901 ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga
     6961 tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag
     7021 agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac
     7081 caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca
     7141 tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga
     7201 cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat
     7261 aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt
     7321 tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc
     7381 tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca
     7441 tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca
     7501 aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc
     7561 agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa
     7621 tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc
     7681 tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt
     7741 tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt
     7801 gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc
     7861 atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc
     7921 gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg
     7981 actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact
     8041 aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca
     8101 tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca
     8161 taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat
     8221 agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc
     8281 gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag
     8341 tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa
     8401 caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     8461 acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat
     8521 cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct
     8581 actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
     8641 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
     8701 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
     8761 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
     8821 cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg
     8881 gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
     8941 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
     9001 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
     9061 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
     9121 ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
     9181 ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt
     9241 gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
     9301 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
     9361 acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat
     9421 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc
     9481 agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
     9541 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg
     9601 attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga
     9661 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
     9721 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
     9781 ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct
     9841 tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag
     9901 gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg
     9961 atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct
    10021 tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt
    10081 aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt
    10141 aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt
    10201 atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt
    10261 ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa taatcatagg
    10321 gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa
    10381 ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc
    10441 cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa
    10501 tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta
    10561 acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt
    10621 tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa
    10681 aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt
    10741 catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt
    10801 cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca tactctttta
    10861 aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga
    10921 atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat
    10981 caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta
    11041 aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc
    11101 cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt tcttccattt
    11161 cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa
    11221 ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt
    11281 ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca
    11341 aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa
    11401 aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta
    11461 atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat
    11521 ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa
    11581 tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg
    11641 gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac
    11701 tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat
    11761 cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga
    11821 ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag
    11881 cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac
    11941 catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt
    12001 taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt
    12061 aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt
    12121 ctacataaga aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct
    12181 ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa
    12241 tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg
    12301 caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg
    12361 gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg
    12421 gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat
    12481 gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca
    12541 ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg
    12601 attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc
    12661 ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat
    12721 cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat
    12781 ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc
    12841 attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta
    12901 ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc
    12961 attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat
    13021 attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa
    13081 aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa
    13141 aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat
    13201 agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc
    13261 taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa
    13321 gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc
    13381 catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga
    13441 catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac
    13501 ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt
    13561 tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca
    13621 agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc
    13681 agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga
    13741 aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa
    13801 tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa
    13861 ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat
    13921 ttctcttcct tgtttcttga attccattag taactccccc acgatatatt ttttatttgt
    13981 taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc
    14041 ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg
    14101 caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca
    14161 tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat
    14221 tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt
    14281 ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc
    14341 tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta
    14401 aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat
    14461 caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca
    14521 ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc
    14581 ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca
    14641 caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg
    14701 catttaaatc tatcactata ttatttatat tgattttttc actgatcatt tataagcctt
    14761 ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt gctaataact
    14821 catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca ggttttactt
    14881 tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa tcaattcctt
    14941 ctggattaac gtagacacag attactggct tttttataaa atctacttcg atatgcggca
    15001 tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct ccctctagta
    15061 tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt aaaacgttca
    15121 cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa
    15181 aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg acatctgccc
    15241 ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc ggaatcgtgc
    15301 ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt agtacatctt
    15361 ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat tttgatgttc
    15421 catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa
    15481 gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt atacagaatc
    15541 tctcttcgat ttcctctaat aaatctacac ttatcataaa tgacctttta aaagaatatt
    15601 tgattttgtc cgtataaggg ttatagatat ttactcccaa tttgagtctt ttaactgctg
    15661 cattgagatg taatactaga ttattgatca attcttcatc aggataaaat tcatttaatt
    15721 taattcttac taactcttgt agctcaacac ccatatctaa ataggaaagc ttcgtttgat
    15781 ctaagtcgtg attcatagca tttttttcaa tcgaagtaat gtgaatgtca acataagctt
    15841 gttcatctat tggcacaata attgaaaatg ctttttcaat cattttgatt aaaataagtg
    15901 tattctttga gatttcttct tcagaattat tactacgtga taagaaattg cttgtttcaa
    15961 tcctttctat cgcaatagca aggtggatag ctaaagtttg aaattcataa tctgtaaatt
    16021 tcaattgtgt cttattcatg tatcgattaa gcaattcggc tacttttttc aatatgtcct
    16081 tatttaagaa attattcgct tcaccaatga aattgatatt caagtctaag agatctgttt
    16141 tactgttaga tgctgtaaag ccaccccaat aatagttcac aacttctgac atcaaccttc
    16201 ttttactttt ttcatcagct ttgatgcgca cgccatttcg atctgaaact aaggacatcc
    16261 catgcttgtt tagtcttttt ctaacatctt tcattgtgct ttctaccgtc gacctgctca
    16321 cgtataattc gtcagcaaga ttttgaatct ttagagaatc attagtattc aataatttag
    16381 caaacacata agttagacgg tcctccggag agtctgcgat attgatttta tgactggata
    16441 ataaatttcg aagtgtttct ttttttccaa taagcgaaac gcctttttta ggttgaacca
    16501 acatttgtac atctggcatt ttttggatta tttctttcaa aattaaaata tctttgctaa
    16561 ttgttttcgt agatacatat aattgttctg ataattcttt gtaagttgtg tgctcatttt
    16621 caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc attatcttca
    16681 cctcctgaat acagcttaag tgtacaatta ttatctctat tttcatacag aagaaagttc
    16741 ttgattaatg tagaagtttc taatattctt gattatttca
//
LOCUS       DAYYKC010000063        16717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000063 DAYYKC010000000
VERSION     DAYYKC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.063"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..1465)
                     /locus_tag="ACHBBW_001977"
     CDS             complement(254..1465)
                     /locus_tag="ACHBBW_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153641.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1584..2219)
                     /locus_tag="ACHBBW_001978"
     CDS             complement(1584..2219)
                     /locus_tag="ACHBBW_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9153642.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2355..3008)
                     /locus_tag="ACHBBW_001979"
     CDS             complement(2355..3008)
                     /locus_tag="ACHBBW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153643.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3111..3317)
                     /locus_tag="ACHBBW_001980"
     CDS             complement(3111..3317)
                     /locus_tag="ACHBBW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM9153644.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3393..4139)
                     /locus_tag="ACHBBW_001981"
     CDS             complement(3393..4139)
                     /locus_tag="ACHBBW_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM9153645.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4299..4523
                     /locus_tag="ACHBBW_001982"
     CDS             4299..4523
                     /locus_tag="ACHBBW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM9153646.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGQYFFNLEVQNV"
     gene            4547..5308
                     /locus_tag="ACHBBW_001983"
     CDS             4547..5308
                     /locus_tag="ACHBBW_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9153647.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5320..5577
                     /locus_tag="ACHBBW_001984"
     CDS             5320..5577
                     /locus_tag="ACHBBW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153648.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5669..5920
                     /locus_tag="ACHBBW_001985"
     CDS             5669..5920
                     /locus_tag="ACHBBW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM9153649.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5920..6069
                     /locus_tag="ACHBBW_001986"
     CDS             5920..6069
                     /locus_tag="ACHBBW_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153650.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6183..6590)
                     /locus_tag="ACHBBW_001987"
     CDS             complement(6183..6590)
                     /locus_tag="ACHBBW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153651.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6751..6975
                     /locus_tag="ACHBBW_001988"
     CDS             6751..6975
                     /locus_tag="ACHBBW_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153652.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6985..7179
                     /locus_tag="ACHBBW_001989"
     CDS             6985..7179
                     /locus_tag="ACHBBW_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153653.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7182..8204
                     /locus_tag="ACHBBW_001990"
     CDS             7182..8204
                     /locus_tag="ACHBBW_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9153654.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8167..8982
                     /locus_tag="ACHBBW_001991"
     CDS             8167..8982
                     /locus_tag="ACHBBW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM9153655.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            9000..9782
                     /locus_tag="ACHBBW_001992"
     CDS             9000..9782
                     /locus_tag="ACHBBW_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153656.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9782..10630
                     /locus_tag="ACHBBW_001993"
     CDS             9782..10630
                     /locus_tag="ACHBBW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9153657.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10627..10869
                     /locus_tag="ACHBBW_001994"
     CDS             10627..10869
                     /locus_tag="ACHBBW_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153658.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11012..11380
                     /locus_tag="ACHBBW_001995"
     CDS             11012..11380
                     /locus_tag="ACHBBW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9153659.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11395..11556
                     /locus_tag="ACHBBW_001996"
     CDS             11395..11556
                     /locus_tag="ACHBBW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9153660.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11553..11879
                     /locus_tag="ACHBBW_001997"
     CDS             11553..11879
                     /locus_tag="ACHBBW_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153661.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11876..12691
                     /locus_tag="ACHBBW_001998"
     CDS             11876..12691
                     /locus_tag="ACHBBW_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM9153662.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12830..13141
                     /locus_tag="ACHBBW_001999"
     CDS             12830..13141
                     /locus_tag="ACHBBW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9153663.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13138..13383
                     /locus_tag="ACHBBW_002000"
     CDS             13138..13383
                     /locus_tag="ACHBBW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153664.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13380..13796
                     /locus_tag="ACHBBW_002001"
     CDS             13380..13796
                     /locus_tag="ACHBBW_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9153665.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13793..14131
                     /locus_tag="ACHBBW_002002"
     CDS             13793..14131
                     /locus_tag="ACHBBW_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153666.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14128..14358
                     /locus_tag="ACHBBW_002003"
     CDS             14128..14358
                     /locus_tag="ACHBBW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153667.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14365..14568
                     /locus_tag="ACHBBW_002004"
     CDS             14365..14568
                     /locus_tag="ACHBBW_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9153668.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14565..14861
                     /locus_tag="ACHBBW_002005"
     CDS             14565..14861
                     /locus_tag="ACHBBW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153669.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14938..15351
                     /locus_tag="ACHBBW_002006"
     CDS             14938..15351
                     /locus_tag="ACHBBW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9153670.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15364..15750
                     /locus_tag="ACHBBW_002007"
     CDS             15364..15750
                     /locus_tag="ACHBBW_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153671.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16301..16528
                     /locus_tag="ACHBBW_002008"
     CDS             16301..16528
                     /locus_tag="ACHBBW_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153672.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16525..>16717
                     /locus_tag="ACHBBW_002009"
     CDS             16525..>16717
                     /locus_tag="ACHBBW_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153673.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5667 a         2727 c         3484 g         4839 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gttcgttatt taatggtatt ttaaatgaag ataagcgaat ttacagcatt tttaaatgct
      181 tatataccgc aaattatccc aagtttttgt atatcctacc cgagtttata cccgaaaata
      241 taatttttta gtgttatttt atatcttttt ttaataggtt ttcaaacgct gtagttgctg
      301 tatctttttc tttgcgtaag acatggccat aaacattcat tgtagtatta aaatctcggt
      361 gtcccaatcg tttttgaagc tcttttggag tcatattagg atcgctaagc aaataggtag
      421 ctgatgtatg tctaatgtcg tgaaaacgaa tgtgacgcaa attgtgacgt ttggtgaatc
      481 gtttgaactt cttatacaga taacttccac gtggcaaagt tccatcttgt tttgtatcaa
      541 atataaaata gtgttttaat ttctttatat ttaatttcca acgaatcttt ttattctctt
      601 tgattaattc atctaacata tcaaatagat aagcaggacc ggttactatt ttttcatcat
      661 catcattttt caaacctggc agcaaccgaa tatctgattt tccatccact tctgaaatcc
      721 gctgatgaaa tcttatttct tgtttgtcaa agtcaatatc tttttcttct aaagctgcaa
      781 tttctccttg tcttgctcct gtaataaatg ctagaacaat taaagtttta atttctatgc
      841 tttcctcaaa tgctgctttt agcattgcat gaatttcttc ttccgaataa ggttcttcga
      901 tattcttttt tacgcctttt tcttttggta tttttacatt ttccacagga ttttctttaa
      961 ttgctccgta ctcattttta gccatcaaaa ataagttgtt aacggcgtat aacatacgaa
     1021 gctttgtatt tcttgataaa ggtttatcag agtttctccc aggatctttt gtattgattc
     1081 tttgagcatt aacaacaatt ttctttatca tgtatggttt gatatcgatt agagggataa
     1141 aatcaaattt atcaagaaat ctattctcga tcattcgctt atagttatga taagactttg
     1201 gctctaaatt tttttcggcc tctgaaagcc atatttttct ataaaattca ccaaaagtta
     1261 tattgaacaa atccaacgaa tcatcggtca ttccttcgaa ttgctctaac cagatgttca
     1321 attctttata tgccgctctt ttactacttg cttttatagt tttgcttttt ctttttgccg
     1381 agccatcagg tttgtatcct aatacagctc ttagtctata tgtttcgttg tctacttgtt
     1441 ctaagtatcc tgttacttct gacataattg aatcagctct ctttctctga tacaataggt
     1501 actataaaga agcctatcgt ataggtttgt ttctttctta gaacacgctc gctttggtcg
     1561 gtggggcgtg ttttttatac taattagttg tgaagagcaa ttgaaactgt ctctgttcca
     1621 aagtaacctg tttgtgcttg caatacatta ttttttgcat ttgcttgggc ttcagagata
     1681 tcaaaaacta ttttaccagt ttgttgcata tcaggattta agctttccat aaagaaagag
     1741 ttagtgatat taccattttc atcttgattt gccgacatag atgcagccga atcagcttcg
     1801 aaagtttttc catctgcttt taatttgaag aatgagctat caacagtcac agccttatca
     1861 cctgcatttt ttacagataa gtcaactact aagaaagtgt cttttgcgtt agtaggaagt
     1921 acagatgggc caacttgttt tactacttca acggagttta ctttgtattc cattttccct
     1981 acagcaacgt catcaccgat tttataagtc ttttcttctt ttgttgtagt ttcttttgta
     2041 gtttctttat tagtacttgt tgatgttgca gtagctgaat ctttgttttc accgccactt
     2101 agagcaccac caataccaaa aaacaaaagt actattaata cccaaaacca tacccgttta
     2161 taaaacggtt ttttaacctt atacattttc ccatcttgac ccataacttt ttttgccatt
     2221 taaatattcc tcatttcttg ttataatata tttgtgatct cagaaatgag gtatgagtcc
     2281 gtgttgcagc acgggctttt ttatttataa cttttttaga gattataggc aaaatagtag
     2341 ggcataaaaa tatattattg aattccgtat ttaaaaaatc ctaattgaac ttcaccggaa
     2401 gtcttttgct gtgttgtacg caatgcttct tcttcagaca ttccattctg tactttccat
     2461 gcaacaggcg acatcccgta tttgttaaca aaatcagtaa gtgataaagt gtcagcgtct
     2521 tgctgagcgc ttgtttgttg gtcttctgga ttttgttgag atgctgcttg ttgttctttc
     2581 tgatcttgac tgataatatt gccagcatca tctgtagtca atccattttc ataaagggcc
     2641 acgccgaaag cttcccactc tttgttggac caatttgcac gatcagctgg agttgactgt
     2701 aaagtgcgtt gtttcatctg ttcatatgtt tcttcttgag gtgcggtttg gattgtactc
     2761 tgactggagc ttataactgt tgggctaggt tccgccgtaa cttggttgga gcttgtaact
     2821 gttgaactag aatctgtttt agatgtagat ttgctagtag atgaactggt ttcagttgtt
     2881 tcttttgttt tacttatttt tgtttcttgg ttagaagtgg catctgttga ttcagctttt
     2941 ttattatttg aacaagctga aagtagcaga gcagtactta acaacaacat aacgctaact
     3001 tttttcattt tatactcctc ttttctttga tacaataagt tgtaaagaag cctactatag
     3061 gtttaacatc ccacgtccac aaactttggt cggtgggcgt gtttttttgt ttatagactt
     3121 tcgaaaacta aagtagcttg gattctatcg ccaccaccga aacctttact accaccgttt
     3181 gtagtggaaa tagtgtgtaa tcgataacct ttagaacact gtctatttat agtgttttca
     3241 agttctgata ggttttgcga cccttttcct ataaactttt cttttaatac aacttgcaat
     3301 acaacgtaac ttggcatatt cttttccttc tttctactat gataataaat actaacccct
     3361 agccggagcg ccaactacga ctggggtatt ttttagaata cgactttccc gattattcta
     3421 atctgttccg gtgagacgac tctatcttca tataattcgt ttatagagcg tagaataatc
     3481 gtttgactct cataatcctt atatacttct ttacacgtca caccgtcacc atctatttca
     3541 acgatagcaa tctcaccgtc ttccacgtct ggctgttcct taacaaatac aaattgtcca
     3601 ttcctgataa ctggttccat tgaatcgcct ataataggaa cagcaaaatc agcattgttt
     3661 ggaactcttt caaattcttc ttcctctaca actgtatcgc cataaactaa ttcagtcggg
     3721 ttggcggcag aagctgaatt tttgattgtc ggaagttttt cctttttagg aaagtgcacc
     3781 acgtttcctc gattttgctc ttcgagttgc tgttcagcaa agttgtagac ttttgtttgt
     3841 cttggttttt ctaattgatt gtagattgtt tcaatggatg gaatgttatt tttcttatct
     3901 tctttaggaa catcgtaacc aagcaaccat gcctcactca catttaaagt tttagccaac
     3961 aaataaagct tgtgttggtc gggcgatgac ttaccattga catattgcga tagatgactt
     4021 tttgacattt ttattccagt ttctttttgc aaaggtaaag acatattcaa aatatcaact
     4081 tgctttaaat ttctttcaga cattaactga ttaagtcgtt gctgagtcgt ttgcttcatc
     4141 ttatacctcc tttctgatac ttattatata ccttattgaa cataagttca ataaaaaagt
     4201 taaattaatt tgaactttta tgttgacaat gttttatgca ggagttatac tttggttaca
     4261 aagttcaaaa catttgaact agaaaggagc gaactaatat gagttttgat tattctaagt
     4321 tgtcgggaag aattgttgag aaatatggaa cgcaatacaa tttttcaatc gctttaggtt
     4381 tgtcggaaag aagtttgtca ttaaagttga ataaccgagt accatggaaa agcaccgaac
     4441 ttcaaaaagc cattgaattg ctagatattc caaaagaaga aattggccaa tattttttta
     4501 atttagaagt tcaaaatgtc tgaacttaag aaaggaattc gaaaaaatga acacacaaaa
     4561 atttgaaaac aatctatttg aattagaagt taagacggaa aatggtgaat cgttatttaa
     4621 tgctgaaaca gtagcaagaa gtttgggttt cgtggaaaca aaaaacggta aagaatatgt
     4681 tcggtggaga actgtcaatg gatatttaaa aaaatacctt tcgcaagaag ttgcgaaaaa
     4741 cgactttatc agtgagccaa tggtttacaa attagccttt aaagcaaaca acgtacttgc
     4801 tgaaaaattc caagactggt tagcaacgga agtcctacct caaatccgaa aacacggcat
     4861 gtacgcaaca gatgaactac ttaacaatcc agacttgctg attgaagtag caaccaaact
     4921 caaagaagaa cgcacgttac gattgatagc tgaacaacga gtaaatgaat tacaaccgaa
     4981 ggcagactat tacgaccgaa tcttgaacaa caaaggatta gttacagtta gtacgattgc
     5041 taagaactac ggtatgagcg ctgtatcgtt taataaattg ctacatgaat taggtatcca
     5101 gttcaatcaa agtggcacat ggctgctata cagtaagttc caagacaaag gatacacgca
     5161 tattgagcca tttgattatg aagataaaaa cggcaatcgg caagtaaaaa caaggatgaa
     5221 atggacgcaa aaaggacaca tcttcttata tgaaacattg aagaagaata attacttacc
     5281 gatgattgaa cgtgaacaaa cagcatagga ggtaacacta tgaacgagga aatgagccaa
     5341 acaatcaagg tacaaaaaat gatagatgat ctaacacatg caattgatag ccaagccgat
     5401 aaaatcatca aggaattgca aggacaaaaa gtttgggatg caaaaatgct tttcaagact
     5461 atcaaatttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga agaaaagttg
     5521 gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa cagctgatgt
     5581 tttatagatg acagtttcat ccgaatctat atcattatag attcgattat tgcaatgtac
     5641 gaattataaa tacaggagga aataaccaat ggagcaacta gctttagtta acctctcaga
     5701 ccttaaagct tctttggcag aatctgagat tgcaaatgaa gtgtgggata caaagcaagc
     5761 tgcggaatat ttaaaaacca caacacgcac gctgactaaa gatgccgaat ctggaaagat
     5821 tccagcagcg aaagttggca gagagtggag gtttagtagt atcgctttgt atcagtatct
     5881 aaaaggagga aaaacatggg taaattcaac agagcactag tgttcagcgc acccctaatc
     5941 gtctatgctt taggactttg gggaagcaga caagcattaa tcggcacgat cgtttacatg
     6001 gtttggattt ttatggggct tgatgaagct gagtacagag cgaaaaagcc caccgagggg
     6061 gctgactaaa gtgtggttct gtttattagg tgtttatctc gtggccgttt tagtaaataa
     6121 ctacatgaga aaacgtggcg aatactggta tacatcctat gcggttctag tatgtctaat
     6181 gcttacaatt tttctaatga tttattcaaa ataggtaata ctttgtcaat caaataagtt
     6241 tcaagagatt tttgttctgc atcagtgttg ccgtaaccaa aacggcttaa gaaaattttc
     6301 ataacctcta tatcttcatt ttgaatatat ggcaggactg cataacctga tgcttttaca
     6361 gcagatatat ttttatcagt gttactacca atacaaatac ccacattgtt gagaaatgtt
     6421 gagaaagttg tttgtattgt ttgggttttt gaatcgtgtt tttgtaattc aatttcttta
     6481 tccttcatcg attcaacatg cttattattt ataaccgctg taatcgaagg ggatataaga
     6541 gctactaaag ctaggataat cgaaattgtg atcgtgttat cgaaactcat tttttcacca
     6601 tccagttttt aaaccattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt
     6661 ttgaaaaata aaaaaagaca cgaacgccag caagcatgat cgtgtccaaa agaaaaatct
     6721 tttaaaccat tataacacag gaggaaataa atgatctcag ttaaagggtt aggcgatgaa
     6781 atattcgaag caatgatgag caaagcacaa caggacatca aagaaaaaat catttctgct
     6841 gcagcgcaag gacaaactag ctgcactgtt cgatcgagag gaacgacacc aagttttctt
     6901 ttggcgctag aagatgaagg cattttgaat atcaaacgaa gagatggcag cgtcagattg
     6961 ttttggcaat tttaggaggc cgtgatgaaa gattttgatt ctttaggcgc cagacagcaa
     7021 ccaccaaatg aagctagtcc tgttggagtt gattggcaag aaaatccgtt atatccaggt
     7081 gatacttgct atctaacaga agaaggctat gtaccagtag acgcaattct agagtacgtc
     7141 cagcaacatt atccaaagat tgaattagga ggaatttagg aatggcaaat gatttaacac
     7201 aaacaacaca acgttcatta gatgagcagg taatcagtaa tcttggacgg ttacaagaac
     7261 aaggtttaga aatgccacca ggatatagtc cacaaaatgc gttaaaaagt gcttttttcg
     7321 aactcactaa caatacagga ggaaacttgt tgcagatggc tgcaaacaat caagaaatga
     7381 aaacctccat ttctaatgca cttttagaca tggttattca agggttatct ccagcaaaaa
     7441 aacaatgcta tttcattaaa tacggaaaca aggttcagct aatgcgttca tattttggaa
     7501 caatggcggt ccttgatcgt gtaactggag gggcagatat tacgcctgta gtcgttcgac
     7561 aaggtgatga gtttgaagtt gcaatggatg gaccaaacat ggttgtcaaa aagcatgaaa
     7621 caaaattcga gaatttagac aatgaaatca ttgcagccta tgtagtcatc aagttaacaa
     7681 atggtaaaga gaccacaaca gtcatgacga aaaagcaaat cgatcaaagt tgggctaagt
     7741 caaaaatgaa agattctgga ccgcaaaagg aatttccaga agaaatggct aaacggacag
     7801 taatcaatcg ggcagctaaa acattgatca acacaagcaa cgacaacgat ttattagttc
     7861 aagctgcaaa agatacgtta gaaaatgaat ttgacaatga tcggaaagat ataacgccac
     7921 aaacagaaaa agtggctact ctcgaacaaa aattcttttc aaacaaaaag attactgaac
     7981 caatacaaaa agaaccagat ccgattgtga ttccagatga tatccaaaat gaagtaactc
     8041 gagttgctga tgtacctgcc catccagaaa tagagcaagc acatttgatc gaaaatgagg
     8101 atatcaatcc aattcaagaa gaattgatag atattccaga ctttggacgc gaggaaggtg
     8161 tagaagatgt ctcagaattt gaagacgacg agtacccttt ctgatgagaa ttattactcc
     8221 aatgaagctg actggcagta catgtcaaca tcacaatata agtccttttt aaaatgtgaa
     8281 gccgcagcct tagcaaagtt gaaaggcgat tgggcaccga cgtcggatcc caaagcctta
     8341 ctcgtaggaa actacgtaca ttcttatttt gaatcaaaag aagtacatga agcgttcaaa
     8401 gaagaaaata aatccaaaat gttttctagt aggaagccgt atggattgtt gaaagatttc
     8461 caaattgctg aacaaatgat tgaacgactt aagcaagaag aagcattcat gaacatttat
     8521 caaggtgaga aagaaacgat cgtgactggg gaactatttg gaacgacatg gaaaggcaag
     8581 attgattgct tgaatgttga ggatgagtat tttgttgata tcaaaacaac caaagacatg
     8641 catgagcgga aatggaacga aaactacgga tcaagagaaa cgttcattgt caattttggt
     8701 tacgtacttc aaatggcaat ctatcaagag cttcttttac agcaatacgg aaagatgttc
     8761 atgccaatca tcgctgcggt atcgaagcaa acgcctagtg aggcgaagtt gataacgatt
     8821 gatcaagata agatgagcta cgagctaatc atgctaaaag aaaatattga aagaattgtc
     8881 aaagtgaaaa atggcgaaga aaggccgata ccttgcggta cgtgcgagta ttgtcgaggt
     8941 aacctttcaa ttactggatt cactagcatg gacgatcttt aaaaggaggt gctttgcgaa
     9001 tgaatctagg atacatcaag ttataccgta aagtgactag ttcattcgtt tggaccaatt
     9061 ccgatatgtt caagctatgg atattgtgtt taatgaaggc tagtcatgaa gatagaaaat
     9121 ttttatttaa cggtcaagaa gtacgtttga caagcggaca attcgtcaca ggagcccatg
     9181 caatagcaaa agagtacaac gaaggagtcc cgagtgacaa agcaattgca tggcgaacgc
     9241 tatggagatg gcttaagaaa tttgaaaatg aagaattatt gacaatccaa tcgaacgcta
     9301 gatatagcgt tataacaata ataaattggt ctgactatca atcgagtgac aagccagtga
     9361 caagcgacgg acaatcgagt gacaagccag tgacaacaaa caagaatgat aagaatgata
     9421 agaatgataa gaataataat cctcgcaact ctcgaaaaac acgagagtat gcagatgacg
     9481 atccaaataa aaaattggcc attcttttat taaaactcat tcgaaagaat cgtagtctca
     9541 aagaacccga tttgaacaaa tgggccaata cgattcgttt gacgattgaa gctgacaaac
     9601 gaactggaaa agaagttcaa gacatgattg tttgggctac taaccatgat ttttggtcca
     9661 cagtcatttc atcgccaact agcttaagaa aaaatttcga tacaatggtg ggtcaaaaaa
     9721 ataaaagaaa gcaacaaaat atttctaatg atgagttacc agaaacaggt gaggattggt
     9781 aatgaataaa aaactaagtg caatggctgc accttacggc ggattaaaaa cagcagatca
     9841 taattgcccg aaatgtggtg atccattgta tatctggaaa acaaaaaata aagatggtac
     9901 tgatcgatgc ggtcctacat gtatcaataa gatttgtggt tatcgagaaa tggtaactaa
     9961 aaatcaaaaa gaagctatcc aaaaagcgaa tgaagcaatg aaaaaggacg ctatcaatcg
    10021 aatgcttaac agttcaatga ttacagatga tgccatatgg accttcgatt ttgatggata
    10081 caaagtagtt gatcaggaaa cagcacaaat aaaagcgatg gctcaagaat gggctaaaaa
    10141 aattgtaaat ggtagcacga ttcatgcagt gataaccggt agaacaggag ccggcaaaac
    10201 ccatctaggc gctgcagtga ttaaagaagt catgagggca tctaattata aaattgcttg
    10261 ttcatttatc agttatcgag aacttttaga acagttgaaa tttgcaatga atgatccaga
    10321 agcaagaaaa gccgtaacag gttcgctgat ggctgagata aagaaatcag atttcgtggt
    10381 aatcgacgat ttgggtgcag aactaggaag aatggaagaa aacaaccagg caacgcctta
    10441 tgatgttgat gttctcacat cgctcacaga agctcgtcta aacaaagcta cgatattcac
    10501 aaccaattta tcatcgaagc aattaaaaca cgcgtacggt gagcgagtgt tctctcgtgt
    10561 aatgaatgga acgaaaggga acatagctgt attcaaaaca acgacagaca aaaggaggaa
    10621 tccagtttga cctttgtagt gaaaaaaatg tgctacttag atagccgagg gcgaggagaa
    10681 gctagtgtcg agtttgctaa acaccacacg actaaagaag aggctgacct tgttgcaagt
    10741 gtttgtggcg gcgaagtagt cgaagttatt aaacccaaaa gacgattttc aaaacctaag
    10801 acaaagccag tgaaaaaaga gtgttgttgt ccgaaaagta accaagcatg gatgagaggt
    10861 gcaaaatgac ttgtttaaaa tgcaacgatg aaacagttat ttggtataag acattgcttg
    10921 gatggtcaac ttgtgaacct tgtccaatat gcaatgaaaa tggacgacgt tcgaaagaac
    10981 gactcgaaag actaaagaag gagtatagca aatggcaaca agaagcaaat acggaaacaa
    11041 aaagcacgaa gtagacggca tcacttttga ttctatcacg gaagcagagt attacgatct
    11101 agccttgtgg caagcacagg cgaatggttg gaaagtgaag ctccaggaaa ggtttgagct
    11161 gatgccgaag tttgaactag agggcaagaa gtatcgcaag atcgagtata ttcccgactt
    11221 cacattttac aaaagcggca agctggtcaa agtcgtagat gttaagggga tgcagacaaa
    11281 agactttaag atcaaggcaa agttgttctg tcatcaatat caagtgccgt tgatattagc
    11341 caaaaaatat cggaatacgt tcaaggaaga gcgtttttaa cgaggtggtc catcatgaca
    11401 acagaagaag tgattcaaat gcgaattcga agccttcggc gtgagattga cgatctggaa
    11461 cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt gcacatagag
    11521 aatttaagaa gggaaatccg tagattggag gaatgagcgt ggataaggaa gcggcttggc
    11581 gaaaattaat gttgctgatt caagatgaga actggcaaga agatgaagca gtggttgctg
    11641 aagttcagcg tctaggaaag attgctaacg gacgtatacg aaaaaagcca gacaaaagaa
    11701 aacagcgcaa agggaaaatc gtcgttgttt tacacgaagg caaaattttg atgcaaggaa
    11761 cagctagtga gctgtctgca gaaactggat atacgcgtgg gactattcga acgtacgctt
    11821 ggcgaaatca caccgatcga aaagggcacg aatataagta tttggaggaa gaaaaatgaa
    11881 cgaaaacaaa ttaatcaaat tgggtgtagc aggagcagta atcgtaggta ttggagttat
    11941 cggaggattt aagttcttcg aaaaaatcga taatggatat gtgggtgtgc gctattcaat
    12001 gaacggcggt atcaaagatg aagcactgac gcaaggtgtg aaatttgtag ggattgacaa
    12061 agtgatccaa tatccaattc gcttgcaaac tatccaatca aaaaatattt cagtatctac
    12121 aagcgacggc aaaaagacaa cgattgatat caaatatgac tacaaagttg attcaactaa
    12181 agcagcaaaa atgtacaagg aatttgggaa tatcacttcg gaagatatcg aaagtggatg
    12241 gttaaaatct aagcttcaaa aggtcgctcg tgaagtttat gcgaaatata gtctgcttga
    12301 tgtcctttca ggagattcct ctaaagttga agctgaggta ttaacgaact ttgctaaatc
    12361 agttgaatct aaagggtttg aagtcgaaga cgtaacactt ggtgttccag atgtcgataa
    12421 agaaacacaa aaatcaatcg atgcgatcat tcgagctggt caagaaaatg aaaaagcgaa
    12481 gctagacgca gaaactgcaa aaactcaagc tgatagtgaa gcttacaaga aaacaaaagc
    12541 tgcagaagca gaggcagaat ctaatcgcaa agtcgccgaa tcagtaacag acaatttgat
    12601 tcgttatgaa gaagctcaag ctcgcaaaaa gcatggatgg gtaacagtaa atggagcaga
    12661 tactgtagtt acggatgaag caggcaaata atatgggatt ctttatggct aaaattctct
    12721 tgttcttagg tttagtagga gcagcatatc tcgtgtatgc gctcctttcc caaactgatg
    12781 acaaagaaga tgacaacaac gatgaaatga aattttagga ggagaaataa tggacgaact
    12841 aatcacaaaa gtagagcagt gggctaaaga caagggactg gatcaagcag atccaaaagc
    12901 acagtttttg aaagtagctg aggaattcgg ggaaatagct tcggcgatgg caagaagtaa
    12961 tgatgaacta tttaaagata gcgtaggaga cgttatcgtc acgctgatta tcctttccat
    13021 gcaaaaaggg acaaacgtac aagagtgttt agaaatggca tacaacgaaa tcaaaggacg
    13081 cacagggaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg gcagcaaatg
    13141 ataaaaaagc tcattcaatt cagcatggat ttatatgata tcgaatcagg agcaacacta
    13201 tctgtggaat cggaccatct aatcataaat tttggtggaa agcgccagat tattttgtgg
    13261 gtagttgatg atgtactgtt tccagaaatt gttcatgatt tcgaagaatc aaaagcggtt
    13321 gagtttgaaa tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga ggacagcgaa
    13381 tgataccgaa gtttagagcg tgggataaac gaaagaacgt aatgagagat gtagccgtct
    13441 tgcattttac taaaaacggc aaaacaaact ttattgaata ttggataaat cctaccgaat
    13501 tgaaatcata tcacgtgcga aacatcgacc tcatgcaatc cacaggaatg aaagataaga
    13561 atggtgtgga tatatttgaa ggggatatag tattagtcag cgtgcgaaat ggcttcgatt
    13621 acttagataa taaagtttgt attgtcaaaa attcaataga ttattccgga ttagtttgtg
    13681 ccactgttga tgaagactta gagtatcaaa tttttaacac agagctgttt gaagaataca
    13741 cgtatgaagt catcggaaat atatacgaga atagcgagtt attggaggta gagtgattat
    13801 cttagctact gactacatcg attctttaaa agatgaaaat gggaatgtcc cagcattgct
    13861 aaattgtgga attaaaagca agaatagaat cattttgtta cggatatcgg ctgatttagc
    13921 aaaatatctt tatctgagca agacagcagt atattacact gcggctacac gttatcagta
    13981 caagggaaaa acaatcagtc aggattatta tgatcgttta atcagtcctg atatgtatgg
    14041 gaattcagaa tcagcaatta agctgtttgg cgcaatagag atatatgccg atgattttcc
    14101 aaatttatgg ttgaaggagg aagcggaatg aaatacgaaa taccactaag tgaagcagac
    14161 gtccagtcaa ttattaacgg tcgggaggtt aataaaaaac ttcctgatgg tactgaatta
    14221 gtcatcagac aaagttattt gaaagatatg gcagctccag tattaattga tcgttttaac
    14281 gtgactgatt ctgtggtaga gaaccacttg aaagaatttc gatcaagtat aaacgacact
    14341 ttcagattag ggagttgatt gacaatgaac accagacacc gcagagtagc aaaactaaga
    14401 aaacaggaac tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa
    14461 gaaacatata aagtggcaag tcagtgtgtt gctgatgcaa gtgatgctat tcgtaagttt
    14521 ggaatttcga tattaagtga tgatcgtaaa tgggaggaaa aagaatgaaa ctaaaagacg
    14581 gattttacgc tagcagtcac ggtatcggcg gtttaatgct agatatgccg acaaagaatc
    14641 ctaaaacacg taagaaacca aaagtcaaag tcggtgacat ggtccgctgt gaagcagagg
    14701 agttcattta tccatttcgt ggatatgtag aacacgtcta taatcactca gcgatcattc
    14761 gcattgaaaa cacgatggaa tgtgataaat ggacagctaa aagcaaagag aatttagcag
    14821 tagtgagatt ggtggatatg gaactaatca atgacaaata aaaaagccgg atcgctccga
    14881 ctaacataat aaaacagaca agtttattat atcacataaa aggagcagtt tgaattgatg
    14941 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
    15001 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
    15061 attacggata tgccgaaggc accgaagcac ggcaataagg cagaggatgc attgattcag
    15121 atgatggata tagaagcgga gagagacgcg attttagcgg ctttgatggc tcttagtctg
    15181 attagtcgtc agatactcta ctacagtttt tgtgtgccag acagcttctc aaactacaga
    15241 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaaatc ggaagctcta
    15301 atagagtttg cagaagcata taaacacgga agaataattg cttataaata gggggaccat
    15361 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
    15421 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
    15481 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
    15541 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
    15601 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
    15661 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
    15721 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
    15781 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
    15841 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
    15901 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
    15961 attatctatc atccgttgct gtttattgtt acatacagat cgcttaggcg gtctttttat
    16021 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
    16081 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
    16141 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
    16201 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
    16261 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
    16321 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
    16381 tgttcaaatg aaggcgaagt attcgattgt agaaatgcta agagaagcta caccaaaaga
    16441 gattgatcag tataagttgg ttcttattgg cattggttgg tttaaggatg aacatattca
    16501 aacaaacatg aagaggtggt tgagatgaag ttatattatg ttgaaaaagc aaattgttgg
    16561 gtggttgaag gctgtctgtc aaagtacgct acagatatta tatctactca attttcagag
    16621 ttgggattga aggttatctt ttgtggtgag tttgtcagtg tgaaagaagc ggtattgcca
    16681 atgacagcaa agagcagacg ctcgcgtggt tcaagtt
//
LOCUS       DAYYKC010000064        16146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000064 DAYYKC010000000
VERSION     DAYYKC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.064"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHBBW_002010"
     CDS             226..894
                     /locus_tag="ACHBBW_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9153674.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="ACHBBW_002011"
     CDS             complement(1094..1324)
                     /locus_tag="ACHBBW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153675.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1321..1479)
                     /locus_tag="ACHBBW_002012"
     CDS             complement(1321..1479)
                     /locus_tag="ACHBBW_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153676.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            complement(1451..2833)
                     /locus_tag="ACHBBW_002013"
     CDS             complement(1451..2833)
                     /locus_tag="ACHBBW_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9153677.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHBBW_002014"
     CDS             complement(2989..4176)
                     /locus_tag="ACHBBW_002014"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9153678.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHBBW_002015"
     CDS             complement(4183..5214)
                     /locus_tag="ACHBBW_002015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9153679.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHBBW_002016"
     CDS             complement(5260..6684)
                     /locus_tag="ACHBBW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9153680.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHBBW_002017"
     CDS             6859..7764
                     /locus_tag="ACHBBW_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9153681.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHBBW_002018"
     CDS             7855..8532
                     /locus_tag="ACHBBW_002018"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9153682.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHBBW_002019"
     CDS             8529..8699
                     /locus_tag="ACHBBW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153683.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHBBW_002020"
     CDS             8813..9709
                     /locus_tag="ACHBBW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9153684.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHBBW_002021"
     CDS             complement(9764..11134)
                     /locus_tag="ACHBBW_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153685.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHBBW_002022"
     CDS             11372..12118
                     /locus_tag="ACHBBW_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9153686.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHBBW_002023"
     CDS             12115..12954
                     /locus_tag="ACHBBW_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9153687.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACHBBW_002024"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACHBBW_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACHBBW_002025"
     CDS             14045..14797
                     /locus_tag="ACHBBW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153688.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACHBBW_002026"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACHBBW_002026"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9153689.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5239 a         3009 c         3242 g         4656 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc aaaaattttt
    16081 ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt tcattcaaaa
    16141 ataaaa
//
LOCUS       DAYYKC010000065        16073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000065 DAYYKC010000000
VERSION     DAYYKC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.065"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHBBW_002027"
     CDS             complement(93..1136)
                     /locus_tag="ACHBBW_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9153690.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHBBW_002028"
     CDS             complement(1513..2478)
                     /locus_tag="ACHBBW_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9153691.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHBBW_002029"
     CDS             2712..3557
                     /locus_tag="ACHBBW_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9153692.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHBBW_002030"
     CDS             3748..4935
                     /locus_tag="ACHBBW_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9153693.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHBBW_002031"
     CDS             4941..5297
                     /locus_tag="ACHBBW_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9153694.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHBBW_002032"
     CDS             5299..5637
                     /locus_tag="ACHBBW_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9153695.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHBBW_002033"
     CDS             6032..7066
                     /locus_tag="ACHBBW_002033"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9153696.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHBBW_002034"
     CDS             7059..7745
                     /locus_tag="ACHBBW_002034"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9153697.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHBBW_002035"
     CDS             7769..8617
                     /locus_tag="ACHBBW_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9153698.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHBBW_002036"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHBBW_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9153699.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHBBW_002037"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHBBW_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9153700.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHBBW_002038"
     CDS             10883..12118
                     /locus_tag="ACHBBW_002038"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9153701.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHBBW_002039"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHBBW_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9153702.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHBBW_002040"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHBBW_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9153703.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHBBW_002041"
     CDS             complement(14069..15211)
                     /locus_tag="ACHBBW_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153704.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15262..16059)
                     /locus_tag="ACHBBW_002042"
     CDS             complement(15262..16059)
                     /locus_tag="ACHBBW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153705.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         5219 a         2963 c         3381 g         4510 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagttaaaaa
    15301 ttttgcgatt atactatttt tctcatatgg gaaattgtat tttttactaa gatctgaaat
    15361 aaatttttct gtttcaaatt ggtttttagt aattttataa aagaaatcct cgacagatga
    15421 atactttgaa atatctttat aggctaagtc catttcgtct aatagctgtt ttaaagaatc
    15481 aatcattctt tctttttcac cgtatttgaa atcaccattt cttatcttgt cttgaagttt
    15541 actgttggct cttttgaaaa atagctcttt agagatagag tgtctttggt tagcgtactc
    15601 ttcaatcaga ggattgatat tattagcaag tatctcattt agataaattt caaaatcttt
    15661 tgagtaatat tttgtaaata tattttttat tataggattc aataactcat agtctttatc
    15721 tagcgaaaac gtatcactat atctttcttg aatgtcttta aagactttgt gaggtatttc
    15781 tgggaagtcc ttaatatata gctcataccc tatatcgata aggtatgaag ttatatattc
    15841 ttctaaagtt ccccatttaa tccagtcaat tgttgaatta gtgatagttg ataataattt
    15901 cagcttttgt ggatttggta aattggtccc acgttcccag ttggatatgg tcgcacgtgg
    15961 tgaattatct attttccttc caagttcttc catagtaaaa ccacatgaaa ggcgtaattg
    16021 tttgattcgt tttcctgctt ctgaaggatt tggcttcatc ttttttctcc ttt
//
LOCUS       DAYYKC010000066        15178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000066 DAYYKC010000000
VERSION     DAYYKC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.066"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(534..1100)
                     /locus_tag="ACHBBW_002043"
     CDS             complement(534..1100)
                     /locus_tag="ACHBBW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9153706.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1100..2056)
                     /locus_tag="ACHBBW_002044"
     CDS             complement(1100..2056)
                     /locus_tag="ACHBBW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9153707.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2056..2712)
                     /locus_tag="ACHBBW_002045"
     CDS             complement(2056..2712)
                     /locus_tag="ACHBBW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9153708.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3082..5100
                     /locus_tag="ACHBBW_002046"
     CDS             3082..5100
                     /locus_tag="ACHBBW_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9153709.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5170..5376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5527..7941
                     /gene="leuS"
                     /locus_tag="ACHBBW_002047"
     CDS             5527..7941
                     /gene="leuS"
                     /locus_tag="ACHBBW_002047"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9153710.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8289..9842
                     /locus_tag="ACHBBW_002048"
     CDS             8289..9842
                     /locus_tag="ACHBBW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9153711.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10223..12007)
                     /locus_tag="ACHBBW_002049"
     CDS             complement(10223..12007)
                     /locus_tag="ACHBBW_002049"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153712.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12022..13743)
                     /locus_tag="ACHBBW_002050"
     CDS             complement(12022..13743)
                     /locus_tag="ACHBBW_002050"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153713.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13843..14604
                     /locus_tag="ACHBBW_002051"
     CDS             13843..14604
                     /locus_tag="ACHBBW_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9153714.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4948 a         2776 c         2983 g         4471 t
ORIGIN      
        1 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
       61 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      121 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      181 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      241 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      301 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      361 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      421 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      481 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      541 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      601 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      661 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      721 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      781 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      841 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
      901 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
      961 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1021 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1081 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1141 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1201 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1261 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1321 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1381 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1441 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1501 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1561 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1621 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1681 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1741 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1801 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1861 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     1921 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     1981 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2041 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2101 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat
     2161 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2221 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2281 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2341 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2401 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2461 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2521 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2581 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2641 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2701 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2761 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2821 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     2881 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     2941 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
     3001 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa taatagataa
     3061 attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa
     3121 cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta
     3181 ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct
     3241 gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct
     3301 caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta
     3361 gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata
     3421 aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa
     3481 tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac
     3541 aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg
     3601 aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact
     3661 gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat
     3721 gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga
     3781 agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact
     3841 tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt
     3901 atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga
     3961 caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac
     4021 aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa
     4081 tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg
     4141 gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta
     4201 atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat
     4261 acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat
     4321 acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct
     4381 cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact
     4441 gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca
     4501 aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa
     4561 gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc
     4621 gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt
     4681 ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg
     4741 aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg
     4801 ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa
     4861 tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt
     4921 tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct
     4981 ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa
     5041 tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa
     5101 aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta
     5161 cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca
     5221 tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat
     5281 acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt
     5341 aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt
     5401 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat
     5461 tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga
     5521 cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa
     5581 acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg
     5641 tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg
     5701 gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg
     5761 gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag
     5821 tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg atttagttat
     5881 gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc
     5941 tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta
     6001 cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt
     6061 ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct
     6121 gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa
     6181 agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat
     6241 aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt
     6301 cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta
     6361 gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa
     6421 gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc
     6481 ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt
     6541 ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca
     6601 gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac
     6661 tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta
     6721 gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc
     6781 cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca
     6841 gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc
     6901 ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta
     6961 acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg
     7021 tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg
     7081 aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt acttcattta
     7141 ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa
     7201 ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca
     7261 aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca
     7321 ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat
     7381 ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac
     7441 aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat
     7501 caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc
     7561 caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc
     7621 gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca
     7681 atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca
     7741 cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc
     7801 aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata
     7861 gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta
     7921 gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa
     7981 tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat
     8041 taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt
     8101 cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag
     8161 tattttttga tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt
     8221 attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg
     8281 agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta
     8341 tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt
     8401 tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag
     8461 tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg
     8521 gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc
     8581 aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata
     8641 aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt
     8701 ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct
     8761 acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa
     8821 acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga
     8881 gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat
     8941 acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa
     9001 ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg
     9061 taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct
     9121 atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc
     9181 gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca
     9241 catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc
     9301 tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc
     9361 agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat
     9421 cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca
     9481 cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta
     9541 caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac
     9601 gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc
     9661 tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt
     9721 atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa
     9781 ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt
     9841 aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa
     9901 acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa
     9961 gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg
    10021 ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact
    10081 taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc
    10141 tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg
    10201 atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa agactggcat
    10261 aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct
    10321 tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac
    10381 ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat
    10441 ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag
    10501 caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg
    10561 tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt
    10621 cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc
    10681 cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg
    10741 aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac
    10801 gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg
    10861 tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac
    10921 caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg
    10981 gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca
    11041 ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat
    11101 tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca
    11161 tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat
    11221 agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt
    11281 cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag
    11341 ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa
    11401 tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata
    11461 agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg
    11521 ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga
    11581 gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg
    11641 gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta
    11701 acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga
    11761 tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa
    11821 tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg atcaagggca
    11881 tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat
    11941 gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct
    12001 tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg
    12061 gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac
    12121 aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt
    12181 ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc
    12241 ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca
    12301 tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa
    12361 tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa
    12421 ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa
    12481 taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg
    12541 agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag
    12601 aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc
    12661 tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa
    12721 tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt
    12781 gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga
    12841 ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt
    12901 cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac
    12961 aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat
    13021 tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa
    13081 gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat
    13141 cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa
    13201 agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga
    13261 gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac
    13321 gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt
    13381 atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt
    13441 cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc
    13501 ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac
    13561 aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt
    13621 gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat
    13681 catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga
    13741 catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag
    13801 gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg
    13861 attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa
    13921 caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat
    13981 gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag
    14041 atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa
    14101 agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa
    14161 aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc
    14221 ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta
    14281 cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat
    14341 ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa
    14401 aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt
    14461 aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg
    14521 atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga atattatcat
    14581 gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt
    14641 gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta
    14701 gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt
    14761 tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat
    14821 tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata
    14881 ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat
    14941 tacacttaaa atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat
    15001 agatggattg aataaacata ttttttaatc aaatagtccc tactgtgact ttcctcagct
    15061 atcggtgata ctaccgatag catttttttg cttataaaat aattataagt atttagagat
    15121 ttttcttcta acattcttca aacaagaaaa atcacaaagg ttattgttat ttttatat
//
LOCUS       DAYYKC010000067        15152 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000067 DAYYKC010000000
VERSION     DAYYKC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.067"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(735..1076)
                     /locus_tag="ACHBBW_002052"
     CDS             complement(735..1076)
                     /locus_tag="ACHBBW_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9153715.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1076..1306)
                     /locus_tag="ACHBBW_002053"
     CDS             complement(1076..1306)
                     /locus_tag="ACHBBW_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9153716.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1593..1931
                     /locus_tag="ACHBBW_002054"
     CDS             1593..1931
                     /locus_tag="ACHBBW_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153717.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2136..2698)
                     /locus_tag="ACHBBW_002055"
                     /pseudo
     CDS             complement(2136..2698)
                     /locus_tag="ACHBBW_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3430..4011)
                     /locus_tag="ACHBBW_002056"
     CDS             complement(3430..4011)
                     /locus_tag="ACHBBW_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153718.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4194..4820)
                     /locus_tag="ACHBBW_002057"
     CDS             complement(4194..4820)
                     /locus_tag="ACHBBW_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9153719.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4842..6173)
                     /locus_tag="ACHBBW_002058"
     CDS             complement(4842..6173)
                     /locus_tag="ACHBBW_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9153720.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6328..6729)
                     /locus_tag="ACHBBW_002059"
     CDS             complement(6328..6729)
                     /locus_tag="ACHBBW_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9153721.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6815..7021)
                     /locus_tag="ACHBBW_002060"
     CDS             complement(6815..7021)
                     /locus_tag="ACHBBW_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153722.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7116..8618)
                     /locus_tag="ACHBBW_002061"
     CDS             complement(7116..8618)
                     /locus_tag="ACHBBW_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9153723.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8636..9091)
                     /gene="argR"
                     /locus_tag="ACHBBW_002062"
     CDS             complement(8636..9091)
                     /gene="argR"
                     /locus_tag="ACHBBW_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9153724.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9189..9806)
                     /locus_tag="ACHBBW_002063"
     CDS             complement(9189..9806)
                     /locus_tag="ACHBBW_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9153725.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10027..11400
                     /locus_tag="ACHBBW_002064"
     CDS             10027..11400
                     /locus_tag="ACHBBW_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9153726.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11475..12587
                     /locus_tag="ACHBBW_002065"
     CDS             11475..12587
                     /locus_tag="ACHBBW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9153727.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12659..14296)
                     /locus_tag="ACHBBW_002066"
     CDS             complement(12659..14296)
                     /locus_tag="ACHBBW_002066"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9153728.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14464..15072)
                     /locus_tag="ACHBBW_002067"
     CDS             complement(14464..15072)
                     /locus_tag="ACHBBW_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9153729.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4149 a         3123 c         2660 g         5220 t
ORIGIN      
        1 atagtgtacc taaaagctct actgacataa gatcgtgtaa tcggtaaagt aattttatta
       61 attatacggg atgtaatcaa ttagagaata aactaatcat aaaaggtact tttacattca
      121 taattgtcgc tttgagattc tactttggtt cactatctga cccaattgca cattttcgat
      181 cattacaagt attggtgtcg ttagaacaac taaataaaaa ctagcggtag atttttcaga
      241 atctatgcta atttctgtat ggatgttcca gtgatggcaa atttctcttt tcattgtttt
      301 tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat tctttcctag
      361 acagatcttg atcccaaatt ttacgttttt gagcatcgat atagtttaaa atgacattac
      421 tcatatctaa agagtttaga ttttttagga ctaaaactgt cagttgttct ttttcttctt
      481 tctgttaaag tattgtcgcg acttttcgta tacgttaaga tatgtcgtga aaaaacaagt
      541 tttgtgacac aaaaaaacga atgacttgag gaaatcacct caatcattcg tttaaaatgc
      601 tatatattct ttagtttaag atacggaaat taatgccatc gaccacacta gtagaatgaa
      661 cgacatttac ttggcctttt tttaatttcg ttatgtttag catataaaaa tattatagag
      721 tttacttaca atgcttaaaa catatactgt ataatctgat caattttagc catgtcttgc
      781 aatggtaagt tttctatttt tttgagtttt cgttccttaa aatcaagaga ttttaattgt
      841 gtaataatta cttgtccttt tatatctagc tcttcaggta atgagtaacg tgtcggtaaa
      901 ttctttatag tggaagtaat tggacatatg accgaaaaca ttgttttacg gttaaattca
      961 taacgagaga cgactagtcc tggtcgtctt ttttgaattt cttttcctac ggagggatca
     1021 aaatcaatcc atacaatatc acctttttta ggaatataaa ttctttcttc tgacactata
     1081 aaatttctct cccttcagga gttaaatctt cccattcgtc tttctcataa tactcggcct
     1141 cttccccacc actaaatgga tcttcaattt ttggaactaa cgtaattgtt ccatcatcag
     1201 aatacacaac gatatattct tggttttctg aaggtttctt tccgttatca gaaggtaaag
     1261 ttaccactac agaacttcct tgtaatctag attttgtaac aagcattatc atcactccct
     1321 taataaaagt atactatata acactaagta acgcaaaatt cccctttgta aactatctag
     1381 aaaattgttt tgtttaaagt atatcttatt ggatataaat agaaataaat tgtagccagt
     1441 tcattttttc tttaactaaa aaaataatcc aagaaacatt gaacctatgg atccaaaagc
     1501 tacccaaata ctatagctaa ccccccgcta ccttcaattt tgcagataaa acgctttggg
     1561 agaaaatttc aaagacaaca ggccatccta tcatgcccga ttataaacaa aaagctggct
     1621 attccttttg ctctgacaaa tttcaattgg gaaagttcac gaaactagca ggtgaagaag
     1681 tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga aacattggtt caacgaattt
     1741 caactagaca agactttgct attgtagaac gtgagatcgt agctattttt gtagaagaag
     1801 ggattttaca tgatccaact catatcaatg tggataaatg gcaacctctt atctataaat
     1861 tcagagaata tacaactgcc aaccagtcgt taactttgaa ttttagattc caagaattca
     1921 aagagtcata atgataaaca tcgttaggta taaataaaaa agactcgcag cacgagtaaa
     1981 aaacaaatag aagctataca ttagaaaggt cgactgtgac cctatagatg cagtatatgc
     2041 agcttatgag catttcttct gttctctttg tttttgggat ctgtgctgaa gtctttttcg
     2101 ttttttgccg ctctttcttt gaaaaatcga ttatgttata ccttctccat tactaattca
     2161 ggcatctgct gagtaatgat ttttcgcgca aaagaaatat ctgttaccca aggttcgatt
     2221 tgttcttcag tcaagacaag cggcattcgg tcatggattg gtgcgactgt gcggttaggt
     2281 tcagtagtca ttatgattga ttctgccatt ttttcatcct gatggctacg ataaaaaccg
     2341 ccaatataca ctattttttc cttatttgaa aacataaatt tgtctttttc ttcattccac
     2401 tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta
     2461 ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta aatccccact
     2521 tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca acaagatttg
     2581 acggaaaaac ttctccagct gctaattctg ctgcctgctt cggttgtttc gtctgggctt
     2641 ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga ccacacatcc
     2701 taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc accaacaaaa
     2761 cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg
     2821 gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctgc
     2881 tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc tctgggtaaa
     2941 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagct
     3001 attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa cctcttcctt
     3061 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagc
     3121 agctattttt atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc
     3181 cactaaacgg tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga
     3241 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt tcacaacctc
     3301 taatacacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt
     3361 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
     3421 ctctaattat taaagaacct tttgcgaagt taacaatctt tctttgatca attgaaaatt
     3481 ctgcctaagt agtgatagat cctgtgtgac tttcaacaat aagatacttc cctggatttc
     3541 agcaatcaac gctttggctt gacggtctgc ttcttcatcg gacagttttt cttcagaaag
     3601 tgccaggata ttttgtttcg tatacttgac ccattgattg actaattcta gtagcaacag
     3661 acggtattct tcatccactg tactcaactc aataatcaaa ttacctacag gacagccatg
     3721 ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc aacatttctt ctaatgcttc
     3781 ttctggctgt tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat
     3841 atgcgcatgg atcaccgcta gacaaacttc tttttttgaa gaaaagtaat aataaaattg
     3901 accttttcct gtatcagaag ctgctagaat atctcggatt gacgtttcat gatagccttt
     3961 tttataaata agatctttgg tcgcttccaa gatcttttcc tttgttttca atgatattct
     4021 cctttttcat ttctcatagt cctatattat acagtaaatt catcattttt tcttcttaat
     4081 cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa ctgttctcat
     4141 atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct atcttatgat
     4201 agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc aaagttcgtc
     4261 aacgctgcat ccattgcttc ttggccagga gcaccaattg catctgtcgc taccacaaca
     4321 ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt tccgttcatt
     4381 ccaccaagaa ttactgtgtc gattccattt tttctcaatt ggagcgccaa atcattgctt
     4441 tctggtccaa agattttatg aggagagctg atgatcgtat tttcatccaa ataaggtttc
     4501 aattcttcga taaaatcggc tcctgaacct tcttccactg attcatattg actttttcta
     4561 gcaaacatct gctgctcaag catcatatgt tctcctgcac cgccaaattg ccatttatgg
     4621 tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc ttttgcttgt
     4681 tttaccattt caatcatatt ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa
     4741 ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag aactactgct
     4801 gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa tacatcattt
     4861 agagcttcac tcattgtcgg atgagaatag atttgatctc tcaataacgt ataaggaagt
     4921 tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc cgcatagata
     4981 cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac acctttagga
     5041 tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa tttgtaattg
     5101 acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt cggagtgata
     5161 aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc gcccttcaac
     5221 tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa tccgcctttg
     5281 acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc cacagcgatt
     5341 gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc tgtacttggt
     5401 tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg gccatcttct
     5461 gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga tacacctaga
     5521 tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat atcttcatct
     5581 tcacgtggaa gaaattcaga atgggcatca agaacgacaa ctttagatcc gtattcattg
     5641 aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa tttttccggt
     5701 aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct
     5761 ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc gccttcaaca
     5821 atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa acgtgctgta
     5881 ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt ggcgcgaagc
     5941 atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc tctttctcca
     6001 ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc gccatacata
     6061 cgtgttgact tttcaacgac taaaaccgat tcaccttttg aagctaacgc cttcgctaaa
     6121 gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat catttttccc
     6181 tcgcttcttt tttacctacc gattggtaca aaaatatgat aaccgataat tgtctattct
     6241 ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt
     6301 tagtctggtt cgatttcctt tattcttcta aacaatcgtt acttctacat cgatatttcc
     6361 tctagttgct ttggagtaag ggcagaattc atgcgcatct tctgcaattt tttgggcttc
     6421 ttcttcgctg atgccatcga tatgcacttc taatactaca ccgatctgat agccgccatc
     6481 atctaaatta taaagagaga cacgtgcttt tactgtggac tctgctgtca ttcctgcttc
     6541 tttgatcata tgttgtaccg ccccattgaa acaagaagca taacctgctg caaataattg
     6601 ttctggattt gttccttctg catggattcc tggaggagtg attttcatat gcatcgagcc
     6661 atttggtgcc tcgacttctc cattgcgccc gccatggttg atcattgctg tttcgtatgc
     6721 ttttttcatt gtactgctcc tttcgattga ccatttgcta cttggctata attatagtgt
     6781 atgacaagta aggcgctttc tacaaaaatt ctgcttattc gaataccaga atgccgtctt
     6841 ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc atatttgttc
     6901 caaaataaat agcaatcttt tctacataat cttttatatg attgatttta agatacatat
     6961 ataccagaaa gttgacccca taaaccaata ctgttaaaaa aataaccata ttcgttacca
     7021 ttttttcctc cttttgctgg tctccctacc agcactcgat ttaaccatac cacttttttt
     7081 gccgtccaag caactagcct ttcaaaaaga ctggtttatt ctgctgttaa tgattcaaga
     7141 tcagaaaaat gtccgaataa ttttgctaat tttactaacg ccaacttgtt ctttccgaat
     7201 tcatcatttt ctatctgtaa tactagtaac ggctcatgta agcttaaacg gatcaaaaac
     7261 cagccgtttc cgtatggctc ttctagatca tatcgaatac cttcttcatt ttccgggtcg
     7321 aagttcatcc ctttttcttc ttccaacagt cgcttcattt cttctaatat cttgtttcct
     7381 tgttcgcgtg gttcatcgct gaacaatttt aatcggattt cttgtacttc tacgggctgt
     7441 ttcaactctt tgatttgttc atctaatgtg ataccttttc tttttaattc tggcaaaagc
     7501 atcaggatct ttgcgatgac atatgcacca tcatccagaa aatagttttc cttgaatgca
     7561 gcgtgtccgc ttgtttcgat ggctagctgt gtatctttcc caagacggtt aagttctaat
     7621 gctttattga ttacatttcg atagccggaa atataacgaa cttggatgcc tcctttttct
     7681 tcaatgaatc tttgtagatg agaagaagta ggggaattcg tcacgattct tgttcctgga
     7741 tgctctttta atacgattgt acttaatact gcgatcaaat tatttcgatt gatcactgtt
     7801 ccagaagcac tgacaactgc agaacgatcc acatctgtat cgaaaatgat cccaagatct
     7861 gcttcatttg ctaaaacagc ctgcttgata ctttccatcg cttctttatt atcaggattt
     7921 gggatatgat taggaaatcg gccatctggt tccaaaaatt gggaacctgt tgaatccgca
     7981 cctaagctat tcaatacttt ttcagcaaag aatccaccag caccatttcc ggcatcaaca
     8041 atgattttcc aaccatttag tggtttgctt tctagtgttc caatcccctt tttaattttt
     8101 gtgactaaat cttgcgcata aggtgtcaaa aggtctgctt tttctacgct tccagtttgt
     8161 tcgcttttta ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt ttctgctcct
     8221 ccttctgagg taaagaattt tattccatta taataatagg gaagatggct agcagtcagc
     8281 ataaccgcag catcgcacga gaactgttca aattgagtgg acataaacat agcaggcgta
     8341 gtagctaatt cgaaatcgat gacatgtatc ccttgttctt tcagactttg gatcaacgcc
     8401 tctttcagtg ctggtccgct tatcctactg tcatgaccaa tcccaatcgt ccatttttta
     8461 tttggatcgg ttgatttctt ctttttcttt atccagtttg ctagtcccca gccgatcttt
     8521 tttaccatgt ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc gattcctcga
     8581 atatccgatc cattttgcag cgctttcata tctttcatat aaaagtcctc ctcatttatt
     8641 ccctcatttt ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag cggcaaataa
     8701 taatgaatgt atctacaccg gccatcgttc cgacaacttc agggtaagcc acttcatcta
     8761 gccaattcgt taccacatcg gcaccatttc gttcagtcaa tacgataacc atgaactgaa
     8821 cagactggat acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct tcatttaaag
     8881 aaacagatgg ctggttaaat aatgtatagc gtactatttt gttttcattt tgacttttta
     8941 ctaacttcat ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg gcgcctgctt
     9001 catgcagccg cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta atgatctggc
     9061 ggatcagtgc ttgtcgctgt ttctttttca tattagacac ctctcaatgt tgattatagc
     9121 aaaagataag taatatttct tcatattttg gtatttttta cttttatttt ttgttttcat
     9181 tcaggttgct aacctttttc tattttaaca cgcaaaatcg tcttcatttt ttcaggtgct
     9241 gattttcttg gatcgctgat gtaaatctct ttgtgttctt tagaagctct ttcatagcct
     9301 tcttttgcga tgaatgcttc taatttctca aacgttgctg gttcttcggc atagggcccg
     9361 acgtgtaaga tttgggcaca aagaccttct tccatctttt tgaatactac ttgttctttc
     9421 aagtcttccg gaagtttgct agaacgctga atggcttctt gtgctatcgc ttctgtaaca
     9481 aagtcaggct gtcggatcat caattgataa gaaaaatgtt cttttttcat cactggttca
     9541 tgcaaatact tttcttgcag tccccattgt ccttccagtg gatagacagt atacatctga
     9601 taatcaggaa tcagccaatt ttttttaggc gacatgcgta ttgcatagct gactgcatat
     9661 agacaggcta ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc tctgatgcta
     9721 aagaatgatt ttttaggaac ctctagtatt tgcgggcttt ttttcatact atagctcttg
     9781 tctttacgcc attcaatttt ttccattttt cttcctccgc gaaattccat ttagttattt
     9841 attatcatac ctctttttga gtgacttggc taatcgtgtt gacactattt ttcagtttgt
     9901 gctagaataa gtgaagtaaa taaatcgtac agataacacg tttcggcgtg tcgagagaga
     9961 ggcggtttct atttttataa tagaaatcac tctctttttt tacgaaattt tttagaggtg
    10021 atgtaaatga caaaacaaaa acagctgctt ttagtacctg cagtaattgc aacagggatg
    10081 atgtcttttg cgggcgtatt gatcgagacg gccatgaatg taactttccc aactcttatc
    10141 agacagtttg atttaacgac tgcacaagtc caatgggtaa caacgattta tcttttgatg
    10201 atttcgatca ttgttccgct gtctacttat ttgaatcgca attttagtct caaaaaactc
    10261 tttttagctt caaatctgct tttcttggca ggtataatga tcgatttctt ctctcctacc
    10321 ttcatggtcc tattgtttgg tcgattgctc caaggtgcag cgacaggtat cgctcttccg
    10381 ttgatgttcc atattatctt gacatttgcc cccctcaatc gacgaggcgc gatgatgggt
    10441 gtcggtactt tgacaacggc cattgctcca gcaattggcc ccacttatgg aggcattatg
    10501 acttcacatt ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt gtttctttct
    10561 ttgattatcg gctggtttgc tattcccgag atggcagtac aaaaaagtgg ttatttggat
    10621 tggataagcg ttttaggaat ttgtcttttg tttagtggaa gtctaacatt tttgagtacg
    10681 ttgggaacag tttccagctg gattgcttta atcatcggcg gtattggctg gttccttttt
    10741 tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac tgtctattct ttcaaataaa
    10801 acattccgct tgtttttgat tggcttctta gtttatcagt ttttattact tggtgtatct
    10861 tttgtcttgc cgaatttcct tcagatcgtt caaggaagta gtccttttgt cgctggatta
    10921 gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc ctttttccgg gaaaatgctg
    10981 gatcaatatg gtccgaagaa gccaattttt gctgggctga ttctttcttt gattggttgg
    11041 ctagcattat tcctactcat aggacacgct tcagtgggtg tattgattgc ttgtcatgtg
    11101 ttttatatga tcggtatcgg attatcttac agcaatatga tgaccaccgg aatgaatgca
    11161 ctgaatgaag aactacaagg agacgggaac gcaatgttca ataccttgca gcagttttct
    11221 ggcgctgtgg ctacttcatt agtagcggtc attatcaatt acgtccagca tcatacttcc
    11281 cataactatg aagtgtctac tacattagga tcaaaagcag cattaggcgt cttgttgctg
    11341 ttgcttctcg taagctttgc tcgattcgcc tattatctgt tctttgctaa aaaagcgtag
    11401 attccatata cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa
    11461 aaggaggctt ttttatggaa aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc
    11521 tagccagtta cttagataca tcaaaattca atgttgcttt atttgatgaa gggacaggac
    11581 aggcaactaa agctagcgct gggattatct ccccttggtt gtcgaaacgc agaaacaaac
    11641 aatggtatcg tctggctaaa gacggcgctg cttttttccc taaacttata aaagatttcc
    11701 aacttgatca aacgatttac gcacaatcag ggacactttt gcttcgacct gaaaaagatt
    11761 tagctgaatt ggctgttctt gccgagaaac gaaaagaaga agcgccagaa attggaaaca
    11821 tcgaatggct ttcacctgaa cagacatctc aagcacttcc gttattgaaa ccagcagttt
    11881 cactgcgtat cagtggtggt ggtcgtctgg atggaaaagc atatttagaa catcttggtc
    11941 agcgtttagc acaaaaacaa atggagcaga tccaggcaaa agtcactttg cataagaaga
    12001 gcgattcttg gctcatcaag ggaccgtttg gagaatacac agctgatcgt gtaattttga
    12061 cacctggccc ggcacttaag cctttattac aagaattagg ctatgctgca gatatacgtc
    12121 cgcaaaaagg gcagctctta gcatttcaga catcttataa gatgagtcaa gactggcctg
    12181 ttgctatgct tgacggagaa gctgatttca ttcctttcat ggatggaaag atccttttag
    12241 gtgctaccca tgaaaacgat caaggctggg atttggctcc tactagcgaa gcttataagg
    12301 gattgactaa gagtacagaa cagttcttga ctgatccaga tcagataaaa aactggccat
    12361 acgagttccg tgtaggtacc cgtgcttata cgtcagattt ttccccattt ttcggcgtac
    12421 tgccagatga tccaagctta ctggctgcta gcggtctagg ctcttctggc ttgaccacag
    12481 gtccttatat cggctatcta ttagccaact tcttaaatac tggtcattgg cctacggaag
    12541 attatcaaaa ggacatctcc acctatatta cgaaaaatcc atcatagaaa aacagctttt
    12601 tcaagtaagg atcccgagcg atattgcgca tggattactt cttgaaaaag ctgttatttt
    12661 agttcaattt ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc aagagatctt
    12721 ttcctatctg agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc accagatttt
    12781 tataggacgt atcctcaaaa gaaacaggaa aatctggtat aggtaaatag tggatgtctt
    12841 caaatggacc aaacgacttg aattctgccg tttgttgcac gattctttca acgattgaca
    12901 atgtcgtttc aggtttgatt tttttatcat cgaaatatcc agtattcagg acatagcagg
    12961 ctgtttgctg tttcaaaaac aactctcgaa aagcttgata gtcttcccta agaggataga
    13021 cacggaaagg attagcaaat ggttcaatga ctagctcatc tagctgtctt tttgtagtga
    13081 tattttccgc gtctgatcgt tttgttgcta atgttgcacc aaaagacgct gctaaaactg
    13141 ggtcattgat cttgataatt ggcggaagcg aatcatcttt catgatccaa aaaacagcat
    13201 cgatcttttc gctaagatga tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc
    13261 gcccatttcc gtttcgtata tctcctgtta ctaggacttt ttctccttta cttgtacatg
    13321 tcacgcccac attctgacaa gtaaggaaag aagaaatggc cggatcatca gacggatagt
    13381 cttgtgtttt atcaaagtaa gaaggttcca aggcagtagc aaaaccattt tttcgagaaa
    13441 taacgaaagc atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg
    13501 taatcgttga ttttccagaa ccggacaacc caaatacagc catcgtatag gtctggtcat
    13561 tactagcata ttgtttcatc ccaccgtggc aggcaataaa gccattgcga tttcccaatg
    13621 cccaagcaag cgtcaaagtc gcttttttta attctccaaa gtaacgtaat cctagcacag
    13681 ccgctgtatt atggagagca tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc
    13741 attcgggatc acaaaagata taaagatctc cctcatcaaa ggaaacagac ttttgataag
    13801 tctctaagat cttcgaattc gctggctgaa aattcagcaa ataagaatac aaattgttct
    13861 caaatccttc tggaatcagc aaatgacttt gcacagaaaa ttcttcagat aggccaacga
    13921 ttacttttgt atggtaaaac tggcgcttgc gactttgata cacagcttct cttaaaattt
    13981 tctcgtattt gctttgttcc ttgtctaagt cctcaaagat ccgccgagca cttgctgttc
    14041 ttcccacaat gcttccatca ttgaatacta gtgtcctaac atcagctggt aaatctagtt
    14101 cttcagcatg tgagattgtt aaatctgtga tgatcgttcc tggactccct tttgctagtt
    14161 gataagctgt cttcctatca aaaacttcat gaacattatt accgtaaaat gtcgtttcta
    14221 caatcgatcg aatactggat aacagtggat ttccacttgt gatttcgttt aattcgagac
    14281 tctcttttgt actcatggct cctcctcatt ccctaaacaa aaaaacgttt tctttattat
    14341 acattattcg agtttttatt tgtactgctg cgctgtggga agcaaacgag ttatttttca
    14401 cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt tccttttatc
    14461 ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg aaccataacg
    14521 agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt cgccgtttaa
    14581 gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg tgtatgaacc
    14641 gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg ctgttgcagc
    14701 agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg
    14761 cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg ttgttgctga
    14821 agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc
    14881 gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa atttttgtga
    14941 gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt gagcatttgt
    15001 agttcctaca aacattgcag tagcaccagc agcaaaagtt gtacctaaaa caattttctt
    15061 aaatgaattc atattaaact cctttttttc tccgtttttt attctttcga cgctattaac
    15121 tttatcattt agaaatattt cataggtaaa ag
//
LOCUS       DAYYKC010000068        14961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000068 DAYYKC010000000
VERSION     DAYYKC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.068"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHBBW_002068"
     CDS             5..2377
                     /locus_tag="ACHBBW_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9153730.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHBBW_002069"
     CDS             2438..3898
                     /locus_tag="ACHBBW_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9153731.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHBBW_002070"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHBBW_002070"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9153732.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHBBW_002071"
     CDS             6129..6278
                     /locus_tag="ACHBBW_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153733.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHBBW_002072"
     CDS             6353..6946
                     /locus_tag="ACHBBW_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9153734.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHBBW_002073"
     CDS             6959..7858
                     /locus_tag="ACHBBW_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9153735.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHBBW_002074"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHBBW_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9153736.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHBBW_002075"
     CDS             8724..8984
                     /locus_tag="ACHBBW_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153737.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHBBW_002076"
     CDS             9429..9635
                     /locus_tag="ACHBBW_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9153738.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHBBW_002077"
     CDS             9656..9886
                     /locus_tag="ACHBBW_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153739.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHBBW_002078"
     CDS             9902..10339
                     /locus_tag="ACHBBW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153740.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHBBW_002079"
     CDS             10332..11039
                     /locus_tag="ACHBBW_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153741.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHBBW_002080"
     CDS             11203..11403
                     /locus_tag="ACHBBW_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153742.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHBBW_002081"
     CDS             11419..11598
                     /locus_tag="ACHBBW_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153743.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHBBW_002082"
     CDS             11923..12186
                     /locus_tag="ACHBBW_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM9153744.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHBBW_002083"
     CDS             12180..12530
                     /locus_tag="ACHBBW_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9153745.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHBBW_002084"
     CDS             complement(12554..13168)
                     /locus_tag="ACHBBW_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9153746.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHBBW_002085"
     CDS             13482..13904
                     /locus_tag="ACHBBW_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153747.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHBBW_002086"
     CDS             13914..14117
                     /locus_tag="ACHBBW_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153748.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHBBW_002087"
     CDS             14328..14912
                     /locus_tag="ACHBBW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9153749.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3035 g         3985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg a
//
LOCUS       DAYYKC010000069        14858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000069 DAYYKC010000000
VERSION     DAYYKC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.069"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHBBW_002088"
     CDS             <1..1087
                     /locus_tag="ACHBBW_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153750.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHBBW_002089"
     CDS             complement(1305..2447)
                     /locus_tag="ACHBBW_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153751.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHBBW_002090"
     CDS             complement(2452..2637)
                     /locus_tag="ACHBBW_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9153752.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHBBW_002091"
     CDS             complement(2991..3236)
                     /locus_tag="ACHBBW_002091"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153753.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHBBW_002092"
     CDS             complement(3230..3631)
                     /locus_tag="ACHBBW_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9153754.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHBBW_002093"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHBBW_002093"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9153755.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACHBBW_002094"
     CDS             complement(5649..7415)
                     /locus_tag="ACHBBW_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9153756.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACHBBW_002095"
     CDS             complement(7428..8222)
                     /locus_tag="ACHBBW_002095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9153757.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="ACHBBW_002096"
     CDS             complement(8232..9608)
                     /locus_tag="ACHBBW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9153758.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="ACHBBW_002097"
     CDS             complement(9728..10375)
                     /locus_tag="ACHBBW_002097"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM9153759.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACHBBW_002098"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACHBBW_002098"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9153760.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002099"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002099"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9153761.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="ACHBBW_002100"
     CDS             complement(13042..14673)
                     /locus_tag="ACHBBW_002100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9153762.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         4761 a         2940 c         1991 g         5166 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttt
//
LOCUS       DAYYKC010000070        14225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000070 DAYYKC010000000
VERSION     DAYYKC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.070"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBBW_002101"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBBW_002101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9153763.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBBW_002102"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBBW_002102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9153764.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHBBW_002103"
     CDS             8118..9086
                     /locus_tag="ACHBBW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153765.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHBBW_002104"
     CDS             9104..10009
                     /locus_tag="ACHBBW_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153766.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHBBW_002105"
     CDS             10006..10812
                     /locus_tag="ACHBBW_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153767.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHBBW_002106"
     CDS             10969..11937
                     /locus_tag="ACHBBW_002106"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9153768.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHBBW_002107"
     CDS             complement(12254..12778)
                     /locus_tag="ACHBBW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9153769.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14225
                     /gene="scm"
                     /locus_tag="ACHBBW_002108"
     CDS             13065..>14225
                     /gene="scm"
                     /locus_tag="ACHBBW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9153770.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTS"
BASE COUNT         4030 a         3074 c         2806 g         4315 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagt
//
LOCUS       DAYYKC010000071        13961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000071 DAYYKC010000000
VERSION     DAYYKC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.071"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1207
                     /locus_tag="ACHBBW_002109"
     CDS             146..1207
                     /locus_tag="ACHBBW_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9153771.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1210..2106
                     /gene="murQ"
                     /locus_tag="ACHBBW_002110"
     CDS             1210..2106
                     /gene="murQ"
                     /locus_tag="ACHBBW_002110"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9153772.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2120..4102
                     /locus_tag="ACHBBW_002111"
     CDS             2120..4102
                     /locus_tag="ACHBBW_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9153773.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4167..4613)
                     /gene="nrdI"
                     /locus_tag="ACHBBW_002112"
     CDS             complement(4167..4613)
                     /gene="nrdI"
                     /locus_tag="ACHBBW_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9153774.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4824..6248
                     /locus_tag="ACHBBW_002113"
     CDS             4824..6248
                     /locus_tag="ACHBBW_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9153775.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6429..7202)
                     /locus_tag="ACHBBW_002114"
     CDS             complement(6429..7202)
                     /locus_tag="ACHBBW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9153776.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7199..8104)
                     /locus_tag="ACHBBW_002115"
     CDS             complement(7199..8104)
                     /locus_tag="ACHBBW_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153777.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8258..8578)
                     /locus_tag="ACHBBW_002116"
     CDS             complement(8258..8578)
                     /locus_tag="ACHBBW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9153778.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            9052..9738
                     /locus_tag="ACHBBW_002117"
     CDS             9052..9738
                     /locus_tag="ACHBBW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9153779.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9740..10684
                     /locus_tag="ACHBBW_002118"
     CDS             9740..10684
                     /locus_tag="ACHBBW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9153780.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            10883..11527
                     /gene="pcp"
                     /locus_tag="ACHBBW_002119"
     CDS             10883..11527
                     /gene="pcp"
                     /locus_tag="ACHBBW_002119"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9153781.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            11606..12883
                     /locus_tag="ACHBBW_002120"
     CDS             11606..12883
                     /locus_tag="ACHBBW_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153782.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13067..13816)
                     /gene="pnuC"
                     /locus_tag="ACHBBW_002121"
     CDS             complement(13067..13816)
                     /gene="pnuC"
                     /locus_tag="ACHBBW_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9153783.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         4447 a         2467 c         2956 g         4091 t
ORIGIN      
        1 taggttatga caattctttt gataatataa actcaacctt tagaaatgaa atttaattat
       61 tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag
      121 acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt
      181 gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt
      241 cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact
      301 aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt
      361 ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg
      421 tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa
      481 aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga
      541 agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct
      601 ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca
      661 aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt
      721 ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac
      781 ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc
      841 tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga
      901 atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt
      961 agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa
     1021 agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac
     1081 caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga
     1141 gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg
     1201 aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa
     1261 acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat
     1321 cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac
     1381 acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga
     1441 agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg
     1501 gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa
     1561 gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata
     1621 gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca
     1681 aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa
     1741 cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga
     1801 ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt
     1861 ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta
     1921 atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa
     1981 aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat
     2041 agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc
     2101 ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca
     2161 aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg
     2221 tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc
     2281 aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt
     2341 gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc
     2401 ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg
     2461 ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt
     2521 attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat
     2581 cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc
     2641 aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct
     2701 agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg
     2761 agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt
     2821 atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt
     2881 ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat
     2941 catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat
     3001 gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt
     3061 tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg
     3121 actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct
     3181 attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg
     3241 gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg
     3301 gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt
     3361 tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt
     3421 cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa
     3481 atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt
     3541 cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac
     3601 agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct
     3661 ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc
     3721 acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc
     3781 agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc
     3841 aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc
     3901 atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc
     3961 tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa
     4021 tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc
     4081 tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa
     4141 tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt
     4201 cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat
     4261 gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta
     4321 ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt
     4381 tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga
     4441 acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt
     4501 aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata ttctgctaga
     4561 tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat
     4621 cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg
     4681 aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat
     4741 taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg
     4801 gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa
     4861 ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga
     4921 cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag
     4981 caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag
     5041 gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt
     5101 ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg
     5161 cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga
     5221 gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt
     5281 tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag
     5341 ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac attatattga
     5401 atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc
     5461 tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat
     5521 cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg
     5581 tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc
     5641 ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg
     5701 gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa
     5761 caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa
     5821 atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt
     5881 cattattatt cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt
     5941 tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat
     6001 gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg
     6061 tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc
     6121 tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac
     6181 ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc
     6241 acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa
     6301 catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg
     6361 tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg
     6421 atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg
     6481 atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg
     6541 atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc
     6601 cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa
     6661 aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc aatcatcaac
     6721 actaaactta aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt
     6781 tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc
     6841 agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga ataaataact
     6901 aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata aaggagattt
     6961 ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata
     7021 aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc
     7081 gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat
     7141 aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc
     7201 atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat
     7261 tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg
     7321 ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat
     7381 atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga
     7441 aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat
     7501 tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca
     7561 agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt
     7621 gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg
     7681 caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa
     7741 tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt
     7801 cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga
     7861 tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa
     7921 tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt
     7981 ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga
     8041 catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt
     8101 tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag
     8161 tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa
     8221 aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga
     8281 tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat
     8341 catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag
     8401 gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa
     8461 tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca
     8521 tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac
     8581 ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga
     8641 cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat ctttttctgt
     8701 agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc
     8761 actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa
     8821 gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct atatattatc
     8881 gggcaataaa ttaaaaaaag ctttacctta caataagtaa gataattttt gtgataaaaa
     8941 aataacgaaa aatatttaaa aatagtgtct atttcgtgag aaaacaacta tattttttat
     9001 gtgttttgct ttataattaa aaaaatattg tcattttatg gaggaaaata tatggaaaat
     9061 tatcttagcc ttttaggcgt cttgattgtt attctcgggt ttgcatttaa attagactcg
     9121 atattgatcg taatggttgc tttagttgtt acagctttga ctggcggtct tggcattcaa
     9181 ggaatgttag aacttttagg tacaagtttt gtaaacaatc gcgggatggc gatttttatc
     9241 atcattatgt tggcaacagg aacgcttgaa agaaatggtc tgaaagaatc agcggcttcc
     9301 ttgatcaagc gattcaagaa agtatctgct ggtatgattg tagatattta tgggatcttt
     9361 cgaatgatct ttgcggcttt taacgtgagt tttggtggag ttgctggatt tgttcgtcca
     9421 atcattttac cgatggcatt ggggaccatc gaatcaaaag ggttagaagt ccatcctaag
     9481 catgaagaag aactaaaagg tatggcttca gctatggaaa atgtctgctg gttctttggc
     9541 caagtactat tcatcggagg agcaggggcg ttgctagtcc aatcaacact gaaagatcta
     9601 ggatatgacg taacattggg acagcttgct ttagtggaag ttccagtggc tatcacggct
     9661 ttagttgtgg caagtgtcta cttctatctt aaagaaaaga aattagcgaa gaaatattat
     9721 ccaggaaagg ggcaataaga tggacttttt tacaaatcct gatattttat taggcgataa
     9781 attactggag atcctttata tcgtgatggg actcgtattg atttacactg gagtaagaaa
     9841 tttaacagat aaaaccaatc ctcatcgtta tgggtcagcc tttttctgga cggtcttagg
     9901 ggtagttatt gcaggaggaa gatggcttcc ggcaatcgtg aacggtggct tgatctttgc
     9961 gatgaccata ccagcaattg ctaagaaagt cagcaaagga gaaagccggc ttccatcaaa
    10021 agaatatatg gaaaaaatgt cagacaaact aggaatgaaa atttttattc cagctttgag
    10081 tatcggtgtc tttgcgatct tgtttgctct atttaccaat ttaggtgctt tagtcggcgt
    10141 gggcgtcggt gtttttgcag ccatgctaat cttgatgttc ttttctcgag ataatcgacc
    10201 aagtacattt ttagatgatg cagcagatat gctgggcaca gtagggccgt taagcatgtt
    10261 gccaatgctt ttagcttctc taggtgcggt atttacaagc gctggagttg ggacagtgat
    10321 ttccaatgca gtaggtacga tcgttccaga aggaaatgtg aacatcggca tcatcattta
    10381 cgctcttggc atggtgatat ttacggtaat catggggaat gcatttgcgg ccatcaccgt
    10441 gatgacggtt gggatcggtg caccattcgt atttagtcat ggagcgaatc cagcgttaat
    10501 aggaatggtt gcattgactt gcggtttttg tgggaccctg ctgacaccga tggctgctaa
    10561 cttcaatatc gtgcctgtag cgatgctgga gatgaaagac aaatacggag tgatcaaaaa
    10621 ccagttgttt attgcgttat ttatgctagt tttccaaatt gcttatatga ttctatttaa
    10681 ataatagaag agaggggttg tgacagaata attgtcgcaa cctcttctaa cattgtagtg
    10741 gaacactatt tttttataag aatatattaa gtttgatctg ttatgttatt taaactattg
    10801 tttcctttat ttacgctgtt caaggaaaat ggtatgataa aagataacca tatgttaaat
    10861 caatcggacg gaggaacaac agatgaaagt actagtaaca ggttttgatc catttggtgg
    10921 ggacaaagtg aatccagcct atgaagcagt aaaaaaaatg ccagatgaaa tcagcggtgc
    10981 tgaaatcatc aaagtggaag taccgaccgt attcgaaaaa agcagtcaag tagtcaaaga
    11041 agccatccaa cagcaccaac cagatgtcgt gatctgtgtc ggacaagcag gtgggcggtc
    11101 agcagtctcg ttcgaacgtg tcgcaatcaa tctagcagaa gcaagaatcc ctgataatga
    11161 aggaaatcag ccattcgata cggctttaga agaaaacggt ccagccgctt actttacctc
    11221 attgccaatc aaagcaatga caaaaaatgt acaagatcat ggtctaccag cgtacatctc
    11281 ttatacagca ggaacatttg tctgcaatga tatcatgtat cgtttgcttc atttgatcga
    11341 aacacaattc ccggcaattc gaggtgggtt catccacgtg ccattctcac ccgatcaagt
    11401 gatcgatcgt ccagtgggta cgccttctat gtctttagaa gacattgctt cttctttgac
    11461 ttatgcagtt gaagcagcag tagaaaataa agaagatatt tccggaaatg ccggaacaac
    11521 tcactaattt agcaagaaac actggctggt tgctaacgtt atgttagtag ccagcttttt
    11581 gtaattagaa aggaaagaaa cagccgtgga agaatggcaa tccgtctttg aagaatggtt
    11641 tccaaaagaa atcagcaaaa gctatcccat caaaatttct aaacaataca caagcagtca
    11701 acgttgggag atctatgcaa aattaacgaa aaaacagcgg gaactagtcg ataagcaccg
    11761 ccgatatcta atcagctctc gttttatgga agaacattat ttagctgcaa cagattgggt
    11821 gttttctgac ttcaagatca atccgttttt tcgaacaaaa cgaagccagc aaaaattgta
    11881 ttgtgagtgt ggtagagaat taaaagtgca atacatcgtt aaatcgccaa aaaccggaaa
    11941 gatcctaaaa ctaggcatca accactttgc cgatcacctg catgtctctc cgacagtggc
    12001 agcttcgatc catcagggaa tgacaaaagt tgatctggcc ttagacgaat tattatggct
    12061 taagcagaag aatatcgatt ttcctgaagg actatggcaa aaatactgtt ttgtcttgta
    12121 ccaaaatcgt cggatgaaac agccatattt accggatata aaattagctc agcggctcgc
    12181 agaatttcgc caagtagaaa tgccgattta catagcggat tatcaagcat tggaaaatga
    12241 aatcaagaaa ataagtgaac acatcaatgg gcagtcaaaa aagcgacaga taaaaaaaga
    12301 actatttgac gactttgcag aagaattagt aaaggatgta gaagaattct tgatcaacta
    12361 tcgcgcattt ttacgtaaag attggcaatc catcgtatat gaagaagtgc cagtacatcc
    12421 taatgcttat tttgaaacat ttatttccgt gttgagaaag acaaaacgtc aacgaacacc
    12481 agaagtcact gctcagatgg aatattttgc gaaaaaccaa cgattcatcc aaccaaaaat
    12541 ctatctattt atttggaagc aatattgtcg ctatggtttt acagaagggt tcttcgacag
    12601 cattcctcgg atagtcagaa atggtttttt aaaagtattg cgtaaagaac gagaagctat
    12661 acaatctgct gataagaaag accgtacagt ctcaaaagaa aaatggcaac tagtagtgaa
    12721 agatatccaa tctggaaatg tgcaagagac aatcgacaaa tggaaaggaa aacattatcg
    12781 ttttactgaa gctcagaaac aagcgttgga atattatcag aagttagaag aatcactgcg
    12841 tttcaatgat gaggcaagaa agtatctgaa ggaattacta taaaaaatag aggctgggac
    12901 agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg
    12961 tgaatttcag cttatttccg aaggagttgc ttctgattcc gccgtttata cgtgtttaga
    13021 gcgtgagaca aaagtgttct ttacttttgt cccacgctca gtttttttat gcgtgttgtt
    13081 gtgcgttgta gtattcttct tccatttttt gcatacgttc acgttctttt tggttatacc
    13141 aaggtgaaac ggtaaatgca aggacatcat tcagcaagta aacagagctg ttcacgaaca
    13201 tcgccaaact tgcagatcca tgcgtaaatg aaatatacca aaggaccatt tgagcaagcc
    13261 ctgaagctaa ccaccagtaa tattggttgt tgtaacgcaa gaaactgatg ataccagcag
    13321 tcaagctaat agaaaaagca atggcatcta cccacggccg aggatcatct gtataacgac
    13381 caatcaggaa tcctgaaaca agatagacaa ggaaagtact gatgattgcg ataaaccagc
    13441 tttttccagt gaattttcgg atttttgaag ccatattatg attccattct ttacttaaca
    13501 atacggggat atccaatgtg atcatataag caatctgttc accgatactt aaatagttct
    13561 ttgctgaaaa cccaacgata ataaaacaaa tcgcagacaa aatacctaaa ataccgttga
    13621 ctgattttgc cgcattgatg gataaaatac acaatacacc aagcgtggta ccgataaatg
    13681 taataacgga cagccaagtg atgggactat tgacaagagt catgacctgg cagccaagac
    13741 tgaaaaagaa aagataataa ttttgttgcg gccagccttt tagctgatga gctaaaaact
    13801 gaaaatatga cttcatgtat gaggactcct taagacacta aatgtagtgt gttctgtatt
    13861 tactaacaca atctattgta tgcttttctt gaaaaaaaac tagtctttca attataaata
    13921 atttgtgtta tgataatttt ctgtttgttt tcttgaaaat a
//
LOCUS       DAYYKC010000072        13556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000072 DAYYKC010000000
VERSION     DAYYKC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.072"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1656
                     /locus_tag="ACHBBW_002122"
     CDS             19..1656
                     /locus_tag="ACHBBW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153784.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHBBW_002123"
     CDS             complement(1801..2541)
                     /gene="truA"
                     /locus_tag="ACHBBW_002123"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9153785.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2732..3367)
                     /locus_tag="ACHBBW_002124"
     CDS             complement(2732..3367)
                     /locus_tag="ACHBBW_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9153786.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHBBW_002125"
     CDS             complement(3514..4734)
                     /gene="sstT"
                     /locus_tag="ACHBBW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9153787.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4953..5951
                     /locus_tag="ACHBBW_002126"
     CDS             4953..5951
                     /locus_tag="ACHBBW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153788.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6147..7982
                     /locus_tag="ACHBBW_002127"
     CDS             6147..7982
                     /locus_tag="ACHBBW_002127"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9153789.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8042..8758)
                     /locus_tag="ACHBBW_002128"
     CDS             complement(8042..8758)
                     /locus_tag="ACHBBW_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9153790.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8985..9527)
                     /locus_tag="ACHBBW_002129"
     CDS             complement(8985..9527)
                     /locus_tag="ACHBBW_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM9153791.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9532..9759)
                     /locus_tag="ACHBBW_002130"
     CDS             complement(9532..9759)
                     /locus_tag="ACHBBW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9153792.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9838..11718)
                     /locus_tag="ACHBBW_002131"
     CDS             complement(9838..11718)
                     /locus_tag="ACHBBW_002131"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9153793.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11907..12521)
                     /locus_tag="ACHBBW_002132"
     CDS             complement(11907..12521)
                     /locus_tag="ACHBBW_002132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153794.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12663..13223
                     /locus_tag="ACHBBW_002133"
     CDS             12663..13223
                     /locus_tag="ACHBBW_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153795.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         4248 a         2634 c         2139 g         4535 t
ORIGIN      
        1 tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat catttttatg
       61 ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat ctatcatagt
      121 gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa tgtatggagt
      181 tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat tttttatcca
      241 tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct tgttttaagt
      301 tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta tttttctatg
      361 aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta tacattttcc
      421 gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt cgctttaaca
      481 tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta ccaaaaatgg
      541 tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc tgtcgctatg
      601 gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga ccaaaaaatc
      661 gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag tgcgggattc
      721 ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact gggaaaagaa
      781 ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa ctataacaac
      841 tatactattg gattattctt atttctcgga ttgattggtg cttttatttt tataaaaaaa
      901 ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt ttgttcaacc
      961 aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca atttgtctgg
     1021 cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt gatctattat
     1081 attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct tgcactaata
     1141 ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt aacacttata
     1201 gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta tgcaaacaaa
     1261 gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg taaccaggaa
     1321 ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga taattcgaat
     1381 aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt ggcttctatt
     1441 aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt aactattcct
     1501 ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat tgcttcaaga
     1561 tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt ttctaaacaa
     1621 aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt
     1681 agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac
     1741 attatttaac atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt
     1801 ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg
     1861 ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc
     1921 gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac
     1981 aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac
     2041 tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa
     2101 tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg
     2161 gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc
     2221 caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat
     2281 atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt
     2341 tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc
     2401 aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat
     2461 ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat
     2521 tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc
     2581 taccttttta agattgtagc acaaaatata gctccttgct tctctaagac agaagctata
     2641 caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat
     2701 ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc
     2761 aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca
     2821 gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca
     2881 aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat
     2941 agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga
     3001 tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac
     3061 ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta
     3121 acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga
     3181 tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa
     3241 atacactgat cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg
     3301 gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa
     3361 aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt ataaacgact
     3421 ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact
     3481 cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca
     3541 atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt
     3601 aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat
     3661 aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca
     3721 tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa
     3781 agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg
     3841 tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc
     3901 acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat
     3961 catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc
     4021 tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa
     4081 tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag
     4141 atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca atcctaaaat
     4201 ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg
     4261 atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc
     4321 ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac
     4381 attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt
     4441 ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga
     4501 aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat
     4561 tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt
     4621 caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat
     4681 accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa
     4741 aaacttctct aacaaatgac caccctttca caattttaac aatcattgta tcattttatg
     4801 caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc
     4861 taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta
     4921 ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt
     4981 gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt
     5041 gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa
     5101 caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa
     5161 atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac
     5221 attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt
     5281 tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta acacctatca
     5341 gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt attccttata
     5401 cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta ttatttttaa
     5461 caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca ttacagaatg
     5521 gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg aaagaatgga
     5581 actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg attgttttat
     5641 tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat acagatacag
     5701 cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc tcttcgtttt
     5761 tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat tttcttgctg
     5821 tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat cgcgtatcta
     5881 catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat atcgggcagt
     5941 tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt tatatacaaa
     6001 aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt gaacaattaa
     6061 ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat tttttagttg
     6121 cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca aaagaaggtt
     6181 ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca cccatgatcc
     6241 tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc aatcgttttt
     6301 ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg acagtaatca
     6361 aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg accggattgt
     6421 cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg atccttctcc
     6481 tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat tccttgaaga
     6541 ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt tcgggtgtct
     6601 ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt aaagtaaaca
     6661 agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt caattcctaa
     6721 acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa aaaattaatg
     6781 gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga agtgactggt
     6841 taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc
     6901 ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata acagaaggaa
     6961 caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg gaatgggaag
     7021 gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct tatacccagc
     7081 tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa aaaatagcca
     7141 tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa tttggttttg
     7201 acaggttcta ttatttagac agtccggata aattaactta tacggataaa atagaacaaa
     7261 gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta caaagcaata
     7321 cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct tatgatgatt
     7381 actatagtga attgaactac actgctgaag gaactgctgt cattcctaat agaagaaaag
     7441 aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa aaattcatcc
     7501 aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac catttacccg
     7561 caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag acagattatt
     7621 tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa gaagttgttg
     7681 ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta actccttact
     7741 atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat cctacagaaa
     7801 gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt caaattatcc
     7861 aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt ctgatacaat
     7921 atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa ataaaagact
     7981 aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct tgtttatctc
     8041 attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca ttattcgctg
     8101 tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc accaatactt
     8161 ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat ggttctgtct
     8221 ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca ctttctgacc
     8281 attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc aaaatagctt
     8341 gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt ttgtattgtt
     8401 gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat
     8461 tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa gcctctacag
     8521 aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct gacgaagtag
     8581 aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta gttgttgtag
     8641 tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat tcattcccgt
     8701 tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt tttttcaatt
     8761 aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa tgtgatttta
     8821 ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag aagcacccgc
     8881 tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg tgattttcca
     8941 atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc atcatcgtct
     9001 tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg gtagatgatt
     9061 tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt ttcttctttt
     9121 tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc ttttgcaaag
     9181 ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta aatttttcca
     9241 taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac taactcatga
     9301 gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat
     9361 tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat ttcttctaat
     9421 ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg atggtcgata
     9481 tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga tttacgcctc
     9541 ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag ctaattgttc
     9601 agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa acaaaacttt
     9661 cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca tacagcttgg
     9721 acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc cttccttttt
     9781 cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta tcaagtttta
     9841 cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt taaagataac
     9901 gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa acccggcaaa
     9961 taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt cacgggtatt
    10021 taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat tagattgcat
    10081 caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag caatcccgat
    10141 atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag caacgcgcaa
    10201 tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata attcagcatg
    10261 gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat tatcacctgt
    10321 cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt cagcgacttc
    10381 tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga ttgctacaat
    10441 aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct ttggtgtgag
    10501 agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat gaccatcaat
    10561 ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta attgttgaaa
    10621 atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat gatcggataa
    10681 cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat agttcttgat
    10741 agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc
    10801 gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc gtttcgctcc
    10861 attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc caggacaacc
    10921 aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa caaccaacgc
    10981 aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt tttccgcatg
    11041 ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg tcgtatcatc
    11101 tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc cagagaagac
    11161 ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg cttcatccgc
    11221 ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag tttttactaa
    11281 gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc catcttcttg
    11341 gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact cccgcgtttt
    11401 ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa cgatagctga
    11461 ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga gtaaatcaat
    11521 tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta tgaccgcaat
    11581 aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa attttccgat
    11641 aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat gctcttggat
    11701 ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag actaagtata
    11761 gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag gcggttttct
    11821 tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag tgatcttgcc
    11881 aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag catacatcgg
    11941 agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag tctttcaaat
    12001 ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa tatttctttt
    12061 cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt tgttttcaaa
    12121 tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt taaatatgct
    12181 gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc aaatatattt
    12241 tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg tttctttttt
    12301 tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata caaagcaaga
    12361 atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag attcagcaga
    12421 agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata gatagctttt
    12481 ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc tcctcaactt
    12541 caatgattgt gttatactta cattataata gacacagtgt ctactatcta taatcaatca
    12601 tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa ggagataggt
    12661 aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct gaaacactat
    12721 ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta acagaaacgg
    12781 cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat ctatatgatc
    12841 aattagaaag caactttttt cgggatctaa atgaaattct ctcttatgat ccagcccata
    12901 gctatgagga actatacact cgcttgattg actatgttta cgcaaatact tttttatatc
    12961 gcttatttat cctcaaccat ggtgatattt cgtttcagaa gaaagttaaa caagttattg
    13021 aagaaaacta tctgaacata tggctatatg aagacaagaa aacgattatc acagaagaaa
    13081 tgagattctt tactacgtac cacgtccaag gttgtctatc cgttctcaat cggtgggtag
    13141 aaagcaatta caagtactcc aaaaaagaat tattagaact cttacagaaa ctgaatgatc
    13201 acatagagaa aatcatgcct tgaataaaaa catactaagc ttagttaaga agaaccgtaa
    13261 ccttctcgta taacaactga atcttattta gccaaattgt ttactgaaga ttttttgagt
    13321 aaaatttcta taattcctac ttattgaaaa gtagtttgta gtgctgcttc tccctctcta
    13381 agtgatatat cttgttgtac caatcaaatt cacctcatca ttaataaatt acttttaggc
    13441 taatttatta caacttatag gttgaagctg taaccaattt atagtcatat tatattttta
    13501 tcatccagtt tattgaattc ttctcgttta cctcaattca ttttcaaggg gaatca
//
LOCUS       DAYYKC010000073        13178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000073 DAYYKC010000000
VERSION     DAYYKC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.074"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1081
                     /locus_tag="ACHBBW_002134"
     CDS             158..1081
                     /locus_tag="ACHBBW_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9153796.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1087..2340
                     /locus_tag="ACHBBW_002135"
     CDS             1087..2340
                     /locus_tag="ACHBBW_002135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9153797.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2474..3820
                     /gene="gor"
                     /locus_tag="ACHBBW_002136"
     CDS             2474..3820
                     /gene="gor"
                     /locus_tag="ACHBBW_002136"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9153798.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4755..5708)
                     /gene="menA"
                     /locus_tag="ACHBBW_002137"
     CDS             complement(4755..5708)
                     /gene="menA"
                     /locus_tag="ACHBBW_002137"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9153799.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5721..6824)
                     /locus_tag="ACHBBW_002138"
     CDS             complement(5721..6824)
                     /locus_tag="ACHBBW_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9153800.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6914..7849)
                     /gene="pplA"
                     /locus_tag="ACHBBW_002139"
     CDS             complement(6914..7849)
                     /gene="pplA"
                     /locus_tag="ACHBBW_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM9153801.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8151..10085
                     /locus_tag="ACHBBW_002140"
     CDS             8151..10085
                     /locus_tag="ACHBBW_002140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9153802.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10542..10958
                     /locus_tag="ACHBBW_002141"
     CDS             10542..10958
                     /locus_tag="ACHBBW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9153803.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10972..11538
                     /locus_tag="ACHBBW_002142"
     CDS             10972..11538
                     /locus_tag="ACHBBW_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9153804.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11561..12547
                     /locus_tag="ACHBBW_002143"
     CDS             11561..12547
                     /locus_tag="ACHBBW_002143"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9153805.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12702..>13178)
                     /gene="scm"
                     /locus_tag="ACHBBW_002144"
     CDS             complement(12702..>13178)
                     /gene="scm"
                     /locus_tag="ACHBBW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9153806.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4107 a         2393 c         2713 g         3965 t
ORIGIN      
        1 tttttgtcac aatctctttt tatatttgga cagaatatat cgaactttat tttgattttt
       61 tgtgaaataa ttttcaaaaa tccgacttta aatagaagaa gtattggaaa gtttttagag
      121 ctatgctact attatgaagt tgtaggagga gaaaattatg atcgaactca taaccacagt
      181 agaaactatt gaacaagcag aacagttgct tgctgcaggt gcagatacac tatatttcgg
      241 tgaagagcaa tttggattgc gtctgcctgc ttcatttaca cgagaagaac agcgacaatt
      301 ggtagaatta gcacacaagt atggtaaaaa agcgaatatt gctgtaaatg ggatcattca
      361 tcctgaaaaa atgaaaaatg tacctgaata tttggctttt ttaaaagaaa taaacgtaga
      421 tgtgatcaca gttggtgata caggtattat ttatacgatg aaaaaaaatc ctgaattgcg
      481 tataccgtat atttatgatg ctgaaacatt agtaacgagc gcacgtcaaa tcaatttctg
      541 ggcgaaaaaa ggcgcagttg gagcagtact tgctcgtgaa gttccttttg aagagatgaa
      601 ggcgatggaa gaaaaactcg atattcctgt agaaaccctt gtgtatggtg ctacatgcat
      661 ccatcaatcc aaacgtccac tgctacagaa ttactataac tatacgaaac aagacgaaca
      721 aaaagatcgt gaaagaggtc tttttatctc ggaacctaaa aaagaagaga cacattattc
      781 tatttatgaa gatagtcatg gcacacatat ttttgccagc aatgatctga atctctcgaa
      841 tgaattgaat gaactttatc agcatcatta ctgtacatgg aaattggacg gattatttac
      901 tccaggcgaa aaattcgtaa acatcactcg actattcgcc gaagcaaaaa aacaaatttt
      961 agaaggtagc tgggatcaag aaaaagcgga aaaaatgact agcgaaatca gtaaattgca
     1021 tcctgaaaac cgtgaattag atacaggatt cttttatatt gatccttctg ctatcaaata
     1081 aggagtatga tgaaagaaat gacagtaaaa agaaaattga aacgaccaga aatcctagcg
     1141 ccagcaggaa cactggaaaa gttaaaaacc gcgatccatt atggagcaga tgcagtatat
     1201 attggcggga atgcatatgg tttaagaagc cgtgcaggta attttactta tgaagaaatg
     1261 gctgaaggag ttgcttttgc aaaagaacat gatgcgaagg tatacgttgc tgctaatatg
     1321 gtcacccatg aaggaaatga agatggagca ggggaatttt tccgcagttt gcgtgatgtc
     1381 ggtatttctg cagtgattgt ttctgatcca gctttgatcg aaatttgtgc gacagaagcc
     1441 ccaggtctgc cgatccattt atccactcaa gcttcagcaa ctaattttga gacattggaa
     1501 ttctggaaag aagaagggtt ggaacgtgtc gttttagctc gtgaagtttc aatggaggaa
     1561 gtggcagaga ttcgtaaaaa tacggatacc gaaatcgaag catttatcca tggtgccatg
     1621 tgtatctctt attctggtcg atgcacattg tccaaccata tgtcgatgcg ggatgcaaac
     1681 cgtggcggat gttctcaatc ttgtcgctgg aagtatggat tgttcgatat gccttttggt
     1741 caagaacgaa acagtttaac tgatacaggc gaagtggaag aagaattctc gatgagtgca
     1801 gtggatatgt caatgatcga acatattcca gaattgattg aaaacggcgt agacagcttc
     1861 aagatcgaag gaagaatgaa atcgatccat tatgtatcta cggtgtcaaa tgtctataaa
     1921 gcggctgtag acagttatat ggaagatcct gaaaactatg tctgcaagca agaatggatc
     1981 gacgagttat ggaaagtagc acaacgagaa ttagctacag gtttttacta caatacacca
     2041 actgaaaacg aacaattatt cggtgaacgt cgtaaaatcc cagtatataa atttgtcggt
     2101 gaagtattat cttatgatga ggcaacacag attgctacga tacgtcagag aaacttgttt
     2161 tctgtaggag atgaaatcga attttatggt ccaggattca gtcatttcag tcaaaccgtt
     2221 gaagttatgc ataatgaaga aggcgaatcc attgaccgag caccaaatcc aatgatgatt
     2281 ttaactatgc cagtcgcaca gccggtgaaa cctggagata tgatccgaaa acgtaaataa
     2341 ggaaaaagct gatatatccc tgttttaagc atcttcgcat acaaaattca agaaaagtgt
     2401 tacacttgtt ttctgtagaa aaaggatcgt taaataaagg ttttgattga tctcttcaat
     2461 aaggaggaac aaaatgcaaa tatatgatta catcgtaatt ggtggcggct ctggcggtat
     2521 tgcttcagca aacagagcag ggatgcatgg cgcaaaagtt ttgctgattg aagcgaatga
     2581 attgggcgga acatgtgtca acgtcggttg tgtacctaaa aaagttatgt ggcaagcaag
     2641 tacgattttg gaaacaattg aacgagatgc ctcaagttac ggtatccaag cagatttaaa
     2701 aaatgttgat ttcgccggtc tagtagaaag acgagaaaaa tatattgatt ttctacacgg
     2761 tgcttatcaa agaggattag atagcaatca tgtcgaggct ataaaaggtt acgctacttt
     2821 tatcgatagt caaacgatcg aagtaaacgg tgaaacctat cgtgctccta aaatcttgat
     2881 cgctactgga ggccgtgcaa gtaaaatgac cattcctggt ggagaatacg caattgattc
     2941 aaatggattt tttgaattaa cagagttacc taagagagca atcgttttag gtgctggata
     3001 cattgcggct gaactttcag gtgtatttcg tggtttaggt agtgaagtca tgtgggcata
     3061 tcgaaaagaa cgaccactta gaacgtttga taaaatgctt tcggacaatt tgatccaaat
     3121 gtaccaagaa gctggaataa aaacttacgc acatcatatt gctaaagaaa ttactaaaaa
     3181 caatgacgaa tatacggtgc tatttgaaaa tggtgaaacc ttaactggtg attgtgtact
     3241 ttttgccggt ggacgtgtac cgaataccga aaaattaggt ctggaaaata caaatgtgga
     3301 attggacaaa aaagggttta tcaaagtaga taaataccaa aatacaacgg atgaacatat
     3361 ttttgctgtt ggtgatgtga tcggtaaatt agatctgact cctgttgcaa tcgcagctgg
     3421 tagacgttta tcagaacgtt tatttaatgg aaagaaagat tcttatcttg actataaact
     3481 tgttccgaca gtcgtcttta cgcatccacc aattgcaaca attggtttga cagaagaaga
     3541 agcattggaa aaatatggcg aaaatgaatt gaaagtttat cgttcacgct ttaccccaat
     3601 gtactttgca ttaaatgatt atcgccaaaa atgcgaaatg aaattgatct gtgtcggaaa
     3661 agaagaaaga atcgtcggct tacatgctat tggtattggt gtcgatgaga tgctgcaagg
     3721 ttttgccgtg gctatcaaga tgggtgcaac taaagaagac tttgacaata cagtagccat
     3781 ccatccaaca ggagcggaag aattcgtaac gatgcgataa tttaatatta acagttccaa
     3841 cagcttttta ttgaacaaac catgtaaaac cagaaaatca gtatactgat tttctggttt
     3901 tttgtttttt agttatttat gtaagattgc ttcgataatt ctcaaaacga aataagtagg
     3961 gaagttggag attttgatta tcttcgtaac tgacgaagag taaatgaatc catttggtat
     4021 gcactcttaa gttcaaaata ttggtatcga tgtttaaaag aaatagaaat gtatcgaaga
     4081 acagatgttt aaaaaagtgt gactttgtat aaataaatta gaagaagcaa acgaggatgt
     4141 taaaagaata ccatatagtt accaaaccaa gaaagttctc gtcaagttcg ctggtacgaa
     4201 tcaaatcaag tgaaaaaagg gactgtgaaa aaaattgtgt catagttagt cactttttcc
     4261 gaacaaatag tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga
     4321 ggaactattt ttccatttgt ttctctaata ctcaggacaa aacactgatt ttacaacctc
     4381 tgataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     4441 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     4501 ctgataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
     4561 ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc
     4621 tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     4681 tataaggagc tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca
     4741 acctcttcat gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa
     4801 ctggcattga ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac
     4861 gtttgcttga attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag
     4921 atcggccatt catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac
     4981 atcaatagct ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag
     5041 cgatgatttt caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca
     5101 atcgtaaaca ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct
     5161 accaaagcaa attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat
     5221 gtattgacat aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt
     5281 tcccctaaag gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa
     5341 caacagataa atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg
     5401 ataacaaaaa caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga
     5461 tttctgcggc caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa
     5521 tccatgtaat tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat
     5581 aaaaaagtgt ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc
     5641 gcaaaaggaa atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt
     5701 agtgtcatgg ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag
     5761 tcctccgata atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt
     5821 gtcacgaaga tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt
     5881 ttcacaccca ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt
     5941 atgatcgtga cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa
     6001 tgatggtatt ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat
     6061 gttttgttcc gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg
     6121 atgccgactg tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta
     6181 ccgcccaagt caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg
     6241 tccgtaatgt aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga
     6301 taaacagttt tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc
     6361 tgatcgattt cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt
     6421 gtgattgcac caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc
     6481 cgcttgacta acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca
     6541 ttcacttcat cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt
     6601 actcgatcga aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga
     6661 acgtaagtcc ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct
     6721 gctgaacttt cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa
     6781 gagatgccca gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca
     6841 ttttgaaagg tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag
     6901 tatgattatg tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc
     6961 ctttttcagc tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg
     7021 tagcacctgt aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga
     7081 cgaattcgtc gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca
     7141 tattttcatt atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa
     7201 agtttgattc agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt
     7261 tagagtagtt cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga
     7321 tttcgattgt atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt
     7381 ggaagctttc agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg
     7441 cttttaagaa ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg
     7501 cttccatgtt tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt
     7561 tatcatattt tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt
     7621 aaccatttga ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag
     7681 ctacaacttt tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg
     7741 attcagaaga agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag
     7801 ctgaaaatgc taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc
     7861 accattccat ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt
     7921 agcctataag tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc
     7981 agcaaatttc gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag
     8041 acaaaagaac agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa
     8101 gaattatcat atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc
     8161 aaaatattgt cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa
     8221 agaaactgaa aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta
     8281 tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg
     8341 atctgcagca tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg
     8401 gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc
     8461 tgatccttgg aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg
     8521 gattcaccct ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag
     8581 tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg
     8641 tctgtggttc tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc
     8701 gcctagccaa agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa
     8761 tgccgacgat cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc
     8821 gtaagcacaa cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata
     8881 tcaaattgaa agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca
     8941 aaggaacttc tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg
     9001 ccaatgaata tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt
     9061 tagtctttta tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac
     9121 aaacaggaca cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata
     9181 aatttaaagg aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta
     9241 acctatttga taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga
     9301 atttgaaata cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg
     9361 agtttttcca tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca
     9421 atgtcttatg gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt
     9481 ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat
     9541 catggttcac tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg
     9601 ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg
     9661 ccggagaagc agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac
     9721 caatggctgt gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc
     9781 caaacaatga tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg
     9841 cattagcatt gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg
     9901 tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg
     9961 ctttcgcatt gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat
    10021 ggctacaacg tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga
    10081 aataaataga taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa
    10141 accgtcacag cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc
    10201 atgagcaaga cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca
    10261 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt
    10321 aaattccttc ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt
    10381 gttccacctg ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt
    10441 tctattcttc gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat
    10501 gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg
    10561 ctgaaaaaag tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt
    10621 cttttttacc agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag
    10681 cggtactgcg tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga
    10741 gcaacacata tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg
    10801 atcgagtacg gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg
    10861 caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt
    10921 caacagatgg aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa
    10981 ttacaaaaaa tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg
    11041 gaaaatatga tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat
    11101 ctaatcacga tcgttgccat ttttaccatg aaaaaacgag acagcttctt actactttgg
    11161 ctacgactat tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg
    11221 agtggtgctt tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt
    11281 gtcagtatca tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg
    11341 atggcttctt tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt
    11401 ttagggattt tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg
    11461 cagacacttc aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca
    11521 atgtttacca aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg
    11581 ggaaaactat cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac
    11641 tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg
    11701 aaagctgttg cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg
    11761 agacaaagcc atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca
    11821 tgatgatatc gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt
    11881 tggtaatgtc gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt
    11941 gtctgattac tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt
    12001 attgaatggc gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga
    12061 atatttggaa aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg
    12121 cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc
    12181 tatcgggatg gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga
    12241 aatcggcaaa cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta
    12301 ctctttgcaa aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac
    12361 agaagaagat gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc
    12421 taggaagtta gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc
    12481 cacctctctt cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa
    12541 agactgaata gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt
    12601 ttttgacaaa ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac
    12661 gtccgttagc gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta
    12721 taactcataa aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc
    12781 caattattcg attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca
    12841 ttgcttgttt tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag
    12901 gagacacttg catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa
    12961 gttgatgaat ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct
    13021 gacgtgtccg tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag
    13081 gtagtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat
    13141 gtatccgtac tgtcgcttga agctgacgtg tccgtgct
//
LOCUS       DAYYKC010000074        13174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000074 DAYYKC010000000
VERSION     DAYYKC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.075"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(432..1616)
                     /locus_tag="ACHBBW_002145"
     CDS             complement(432..1616)
                     /locus_tag="ACHBBW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9153807.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1641..2648)
                     /locus_tag="ACHBBW_002146"
     CDS             complement(1641..2648)
                     /locus_tag="ACHBBW_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9153808.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2820..3179)
                     /gene="comGG"
                     /locus_tag="ACHBBW_002147"
     CDS             complement(2820..3179)
                     /gene="comGG"
                     /locus_tag="ACHBBW_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9153809.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3151..3576)
                     /gene="comGF"
                     /locus_tag="ACHBBW_002148"
     CDS             complement(3151..3576)
                     /gene="comGF"
                     /locus_tag="ACHBBW_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9153810.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3539..3886)
                     /locus_tag="ACHBBW_002149"
     CDS             complement(3539..3886)
                     /locus_tag="ACHBBW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153811.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3858..4328)
                     /gene="comGD"
                     /locus_tag="ACHBBW_002150"
     CDS             complement(3858..4328)
                     /gene="comGD"
                     /locus_tag="ACHBBW_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9153812.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4303..4581)
                     /gene="comGC"
                     /locus_tag="ACHBBW_002151"
     CDS             complement(4303..4581)
                     /gene="comGC"
                     /locus_tag="ACHBBW_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9153813.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4578..5627)
                     /gene="comGB"
                     /locus_tag="ACHBBW_002152"
     CDS             complement(4578..5627)
                     /gene="comGB"
                     /locus_tag="ACHBBW_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9153814.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5584..6513)
                     /gene="comGA"
                     /locus_tag="ACHBBW_002153"
     CDS             complement(5584..6513)
                     /gene="comGA"
                     /locus_tag="ACHBBW_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9153815.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6645..8399
                     /gene="ade"
                     /locus_tag="ACHBBW_002154"
     CDS             6645..8399
                     /gene="ade"
                     /locus_tag="ACHBBW_002154"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9153816.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8497..9159)
                     /gene="trmB"
                     /locus_tag="ACHBBW_002155"
     CDS             complement(8497..9159)
                     /gene="trmB"
                     /locus_tag="ACHBBW_002155"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9153817.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9229..10011)
                     /locus_tag="ACHBBW_002156"
     CDS             complement(9229..10011)
                     /locus_tag="ACHBBW_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9153818.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10215..11426)
                     /locus_tag="ACHBBW_002157"
     CDS             complement(10215..11426)
                     /locus_tag="ACHBBW_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153819.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11419..12156)
                     /locus_tag="ACHBBW_002158"
     CDS             complement(11419..12156)
                     /locus_tag="ACHBBW_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9153820.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12294..12728
                     /locus_tag="ACHBBW_002159"
     CDS             12294..12728
                     /locus_tag="ACHBBW_002159"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9153821.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12721..13065
                     /locus_tag="ACHBBW_002160"
     CDS             12721..13065
                     /locus_tag="ACHBBW_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9153822.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         4082 a         2740 c         2210 g         4142 t
ORIGIN      
        1 tttttcgcaa caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca
       61 ggtggaaaat caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta
      121 aacataaagc gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt
      181 tttacatatg taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga
      241 aaatcaataa ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc
      301 taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct
      361 ccgcaaaaaa aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg
      421 aatgctttgg cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct
      481 gttgggatca ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca
      541 cgcaattcat tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt
      601 tcactacgta catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca
      661 ccattcatcg ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca
      721 taagctaaac gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac
      781 aatcctgtta ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata
      841 tcaatctcta atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt
      901 gtacccattg tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct
      961 ccatcgactg ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct
     1021 tcgatcggtt tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca
     1081 tggaagccat attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa
     1141 taattttgct ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg
     1201 tcaggtaaaa tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga
     1261 gcaaattctg ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc
     1321 ccataagttt ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag
     1381 cttaaaattt gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt
     1441 tcgatatcca tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa
     1501 tcttgtaaac cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga
     1561 tataattgcc atttcaaact tgaactccca gcattgattg caattgtttt agacatatga
     1621 ctctccttct cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa
     1681 aattccgtca ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt
     1741 acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta
     1801 ttacggaaca attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac
     1861 cattttttga tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa
     1921 ccaaaaccat ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa
     1981 ctatggcctt cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa
     2041 ccgattggca aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg
     2101 tcttgatgaa agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc
     2161 aacaatgtat catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg
     2221 tttaaaagaa ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc
     2281 ggtgcttttt tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta
     2341 tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt
     2401 aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa
     2461 agagctgtga tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga
     2521 taatcatcta ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat
     2581 gcattctgta aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct
     2641 ggtaacatgg tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc
     2701 tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa
     2761 cattttttta ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat
     2821 cattctgtac tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt
     2881 gagacttcca cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt
     2941 gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc
     3001 tgcttcatta tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa
     3061 cggtagcttt ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca
     3121 gaaaataaaa tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata
     3181 tccatcttct ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa
     3241 ttgaagctca gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg
     3301 tatcattgcc tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg
     3361 ctgatttttc acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa
     3421 tattagccac tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg
     3481 aatcaaaaga ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc
     3541 aatcagcgta aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat
     3601 tgacaaacac tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca
     3661 tttcttccct tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa
     3721 tcattcgaat aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct
     3781 ggccaaagct cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca
     3841 acaaaacaaa tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat
     3901 tgatacgtaa tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt
     3961 tcattcccgg ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca
     4021 ggaatatcta atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga
     4081 ttctcttcac tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc
     4141 gtagaataga ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc
     4201 catgcagaaa atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag
     4261 agtgattcaa acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat
     4321 ttctgcatgt tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct
     4381 tccattattt tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc
     4441 acgactttct tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac
     4501 taatacactg atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata
     4561 agatattttt ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa
     4621 acagcggcat aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata
     4681 aaatgcatcc agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa
     4741 atcagttctt tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct
     4801 tctgatagaa aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct
     4861 tgaatcaact tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag
     4921 actatgattt gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa
     4981 taagccgatt gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca
     5041 ctctgatcaa caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta
     5101 accgctagga tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa
     5161 tgctcttgca tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta
     5221 aacaagatac ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa
     5281 tcattgcgaa atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc
     5341 agattcccat gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt
     5401 tgcgaaagta cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa
     5461 ataacaggtg gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa
     5521 ccagataaaa gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat
     5581 cctttagaat agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca
     5641 tgattttttt actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg
     5701 attggcatct tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc
     5761 ttttttcttt gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac
     5821 ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc
     5881 atctcttatc tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag
     5941 tgtatcataa gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc
     6001 tatctctaca ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata
     6061 cattagagaa gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac
     6121 ggttttctgt aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa
     6181 agtaccgtat aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga
     6241 caaccgcaga cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt
     6301 tcttttttct ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc
     6361 atctgccgaa gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta
     6421 aaatacttct gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact
     6481 tatcatttga tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg
     6541 tctttttgat ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg
     6601 aatttatgtt atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa
     6661 ggaaatatat cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta
     6721 agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa
     6781 tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc
     6841 tagtgccagg attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg
     6901 aattttcaaa agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa
     6961 taggaaacat tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac
     7021 tcatgaatat cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg
     7081 gtgcaaattt aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct
     7141 tagctgaagt catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga
     7201 tccaattgat gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg
     7261 aagagttaaa tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa
     7321 aagaagctaa agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg
     7381 caaaagatct ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct
     7441 ttgtcaccga tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg
     7501 tccgtcaagc tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga
     7561 atgcagcaga atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg
     7621 atttcttctt aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa
     7681 cattagttgt tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat
     7741 atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc
     7801 ttctttcttc ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag
     7861 atttggtaga aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc
     7921 aattaaagat tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag
     7981 tgaaaggatt tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata
     8041 atctagttgt cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc
     8101 aaaaaggtgg cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc
     8161 ctataggcgg aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat
     8221 taataactaa agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt
     8281 ctttcttaac attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat
     8341 tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa
     8401 tcaaataaat cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat
     8461 ggatattaat gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt
     8521 atcttgaaat tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc
     8581 atactccgtc ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt
     8641 gaggatcatg ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc
     8701 tgttttgaaa tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt
     8761 cttatatgtc aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag
     8821 atagatttga tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg
     8881 tagaagtttc aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac
     8941 actgacctgc atttctactc caatataatt gatttcagga tgggcttttg ccattccagt
     9001 gatgaactgg cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa
     9061 ttcattccat tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc
     9121 taacatttcc gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc
     9181 tctaattaaa aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa
     9241 aattcgtttt aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata
     9301 tctggtgatt tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc
     9361 gtcagtcggg cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata
     9421 atttcctaaa atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc
     9481 gactagatat aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac
     9541 tactgataag ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat
     9601 ttgcagaaat tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat
     9661 cttctccggc gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga
     9721 atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt
     9781 cagcaagcga ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt
     9841 tgtccaaaca agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt
     9901 gtttcgttta ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc
     9961 gcctttgatg ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc
    10021 cgtcctctta ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc
    10081 aagcacatat acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa
    10141 aaatagaacg aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct
    10201 acacagcttt gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca
    10261 aagagcatag taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat
    10321 acgactgcag caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga
    10381 tactgaatcg cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata
    10441 tagcggaact ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa
    10501 ccgataatca gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt
    10561 actaatcgga aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag
    10621 taaagataag tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt
    10681 cttcttttga tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag
    10741 atctgatgca acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct
    10801 tgttgcttcc acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct
    10861 gcccatatta gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct
    10921 aatttccaat aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc
    10981 agatgactga atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat
    11041 tgctccgtac taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc
    11101 aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct
    11161 ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat
    11221 aaacagagca tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg
    11281 gttttcaacc aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa
    11341 acaaaatgat cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct
    11401 aaacgttttt gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc
    11461 gtccaaagaa gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc
    11521 tcgtatctct ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa
    11581 aatatgcgta gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag
    11641 caatgcattg atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga
    11701 aggctcgatc aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt
    11761 agctgggaac cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct
    11821 tttcattgct atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct
    11881 caatgtcaat tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata
    11941 ttcttccgta tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc
    12001 cagcagaccg ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa
    12061 ccctactatc tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc
    12121 gtacccccct gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt
    12181 tatttattat accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa
    12241 cttatggtaa agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa
    12301 actgtatttt ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg
    12361 acaaggtcta tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc
    12421 ctaaacgtca tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt
    12481 cgcgtgtgcc taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta
    12541 atatcttgaa taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt
    12601 tagtccctcg ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata
    12661 gttatggatc ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg
    12721 atgattaaaa actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt
    12781 ctgttattgg cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa
    12841 gaagcaacaa ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg
    12901 atcgaaacaa aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa
    12961 gatatcgaag attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta
    13021 gaaaaactaa aagctgatct gccagagtat cctgaacaaa tataataatg attattttct
    13081 caacagattt aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat
    13141 tttgaatttt gtttgaaact accacgacaa cgtt
//
LOCUS       DAYYKC010000075        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000075 DAYYKC010000000
VERSION     DAYYKC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.076"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHBBW_002161"
     CDS             complement(79..2199)
                     /locus_tag="ACHBBW_002161"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9153823.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHBBW_002162"
     CDS             2429..3607
                     /locus_tag="ACHBBW_002162"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9153824.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBBW_002163"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBBW_002163"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9153825.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBBW_002164"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBBW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9153826.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHBBW_002165"
     CDS             complement(4960..6213)
                     /locus_tag="ACHBBW_002165"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9153827.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBBW_002166"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBBW_002166"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9153828.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHBBW_002167"
     CDS             7790..9136
                     /locus_tag="ACHBBW_002167"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9153829.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHBBW_002168"
     CDS             complement(9444..9830)
                     /locus_tag="ACHBBW_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9153830.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHBBW_002169"
     CDS             complement(10098..10247)
                     /locus_tag="ACHBBW_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153831.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHBBW_002170"
     CDS             10709..10921
                     /locus_tag="ACHBBW_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153832.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHBBW_002171"
     CDS             complement(11199..12998)
                     /locus_tag="ACHBBW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9153833.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAYYKC010000076        13022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000076 DAYYKC010000000
VERSION     DAYYKC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.077"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1190
                     /locus_tag="ACHBBW_002172"
                     /pseudo
     CDS             49..1190
                     /locus_tag="ACHBBW_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1316..2395)
                     /locus_tag="ACHBBW_002173"
     CDS             complement(1316..2395)
                     /locus_tag="ACHBBW_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9153834.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2498..2938)
                     /locus_tag="ACHBBW_002174"
     CDS             complement(2498..2938)
                     /locus_tag="ACHBBW_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9153835.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2995..3405)
                     /locus_tag="ACHBBW_002175"
     CDS             complement(2995..3405)
                     /locus_tag="ACHBBW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9153836.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3585..4019)
                     /locus_tag="ACHBBW_002176"
     CDS             complement(3585..4019)
                     /locus_tag="ACHBBW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9153837.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4119..4967)
                     /locus_tag="ACHBBW_002177"
     CDS             complement(4119..4967)
                     /locus_tag="ACHBBW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9153838.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5356..7122
                     /locus_tag="ACHBBW_002178"
     CDS             5356..7122
                     /locus_tag="ACHBBW_002178"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9153839.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7224..8483
                     /locus_tag="ACHBBW_002179"
     CDS             7224..8483
                     /locus_tag="ACHBBW_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9153840.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8630..9931
                     /locus_tag="ACHBBW_002180"
     CDS             8630..9931
                     /locus_tag="ACHBBW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153841.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9934..10785
                     /locus_tag="ACHBBW_002181"
     CDS             9934..10785
                     /locus_tag="ACHBBW_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9153842.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10798..11613
                     /locus_tag="ACHBBW_002182"
     CDS             10798..11613
                     /locus_tag="ACHBBW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9153843.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11764..12930
                     /locus_tag="ACHBBW_002183"
     CDS             11764..12930
                     /locus_tag="ACHBBW_002183"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9153844.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4444 a         2348 c         2432 g         3798 t
ORIGIN      
        1 ctactatctt atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca
       61 accatcttaa tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc
      121 cacacagagg agacagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga
      181 attacctttc aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga
      241 caaacagcga ccatcaaagc aaaaattgac tattcgggag acttagcacc tatactacaa
      301 tcagctgtta catttgagaa tgttgatccc actttgtcga ttggctatga cgagaacact
      361 ttgactatcc aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg
      421 ttaaaaccag cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcattta
      481 acgccgtata gtcagcggca gacaattgag caagattcag ccatcgactc aggaggagat
      541 cttgttgaag aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca
      601 gaaaatccag acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa
      661 actcaaccag agccagacaa taaatcaaaa cctgaaataa cccctggaga aaaaccagat
      721 gtaactccag aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac
      781 acagataatg aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat
      841 gtgactccag aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa
      901 aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa
      961 ataaatgaag agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa
     1021 accttaaaaa gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac
     1081 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     1141 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     1201 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc
     1261 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     1321 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     1381 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     1441 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     1501 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     1561 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     1621 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     1681 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa
     1741 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     1801 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     1861 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     1921 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     1981 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     2041 gatgctcgta aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg
     2101 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     2161 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     2221 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     2281 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     2341 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     2401 gatggaatat gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg
     2461 agttggtttt cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat
     2521 gaaaattatc tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa
     2581 gtggaagata agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac
     2641 cttgagcagt cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa
     2701 tataattttt ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca
     2761 aatcatttcc tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa
     2821 gatactgggt ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt
     2881 ctttagaggc ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt
     2941 ttccacctcc gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc
     3001 actaactgct ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac
     3061 tttatgcgct gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc
     3121 ttcaatctct acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc
     3181 actgacagta gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt
     3241 aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg
     3301 tttaccagga gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga
     3361 gcgtccgcct gtgttgatca ttttcgtaga atacattttt ttcataaagt taccaccttt
     3421 aattatattt tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat
     3481 tggcataaga taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca
     3541 tatacaaaaa atatcagaga caaaaaataa tctcatcata aacattacat atatttctca
     3601 aaaagttgtt ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa
     3661 taaccaaaag gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt
     3721 tcttgaatct ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag
     3781 acgatgatat tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga
     3841 gacgtgtttt catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta
     3901 ttgcctctag ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac
     3961 agttctagtg ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata
     4021 aagatctcct ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag
     4081 aaaacgcatt caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta
     4141 aatccgcgtc ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt
     4201 tcttctatca atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc
     4261 tcttttgact ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc
     4321 tcagcactta agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat
     4381 ttctttcgat tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat
     4441 aaaatagcat aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat
     4501 gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg
     4561 ggagggatcg tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa
     4621 gcaaaaatcg cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct
     4681 ggaactattg caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt
     4741 tttttaccta aaaatagaag agctagtacc aacataacga tattcaacac taaaataact
     4801 atagctcggt caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca
     4861 gcagcaatat gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca
     4921 aaaataatct taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct
     4981 taattcatta ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta
     5041 attaataaaa aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc
     5101 tacgccaaaa actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag
     5161 ataaattcag gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc
     5221 tatctatttg gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca
     5281 tttttgaaag cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac
     5341 acaaggagga aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca
     5401 tttctatata cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa
     5461 aaagtatatg tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa
     5521 caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg
     5581 acgagcgaaa caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac
     5641 tgtttttacg gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat
     5701 ttttattttc gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta
     5761 aaagaaacag tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat
     5821 gaccccgaac acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg
     5881 ggagatttgc aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc
     5941 atctattttt gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat
     6001 tatgaggtgg atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca
     6061 cataaaagag ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca
     6121 ccacagtggc aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac
     6181 atccgttttt tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat
     6241 atcccatatg atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca
     6301 gaagtgcaaa aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat
     6361 ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa
     6421 gcagttacag aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa
     6481 gcttggctgc aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt
     6541 aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat
     6601 cagcaaatgc tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat
     6661 gatactgcca ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg
     6721 gcctttatgt ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg
     6781 actggtgaag atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat
     6841 ctagagctat tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt
     6901 atttctgaag gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc
     6961 acacggaaac tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg
     7021 gtagtagaac aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg
     7081 aaagcagaga tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca
     7141 acaagaaagc gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt
     7201 tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt
     7261 taatctcttt aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag
     7321 aaggggaagc cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt
     7381 atataaacga aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag
     7441 aaaaggacat gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac
     7501 ctgacgttat gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg
     7561 cggaagtaaa actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt
     7621 atgatggaaa aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca
     7681 aagatttagt cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca
     7741 gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg
     7801 acttttatta ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg
     7861 gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg
     7921 caacagattg gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag
     7981 acttcgccag ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc
     8041 aagcgcaaac atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa
     8101 ataatggaca gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg
     8161 ctaaaaacaa agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag
     8221 aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa
     8281 aggctaaaaa cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga
     8341 tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg
     8401 cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg
     8461 aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa
     8521 caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca
     8581 ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga
     8641 taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg
     8701 gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt
     8761 cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag
     8821 tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat
     8881 tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa
     8941 gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg
     9001 tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat
     9061 cattttccca gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat
     9121 tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt
     9181 aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg
     9241 tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt
     9301 tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt
     9361 tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca
     9421 agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac
     9481 ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta
     9541 tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc
     9601 aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt
     9661 cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat
     9721 gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga
     9781 tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt attctatggc
     9841 ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa
     9901 aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga
     9961 ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt
    10021 atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct
    10081 tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa
    10141 acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta
    10201 caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc
    10261 aaatcaagtc tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact
    10321 gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg
    10381 atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact
    10441 gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt
    10501 ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt
    10561 atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag
    10621 actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt
    10681 tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa
    10741 aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg
    10801 caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga
    10861 cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg
    10921 gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt
    10981 ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta
    11041 tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta
    11101 ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag
    11161 gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat
    11221 agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa
    11281 agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt
    11341 cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg
    11401 acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg
    11461 aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt
    11521 atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc
    11581 aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg
    11641 aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc
    11701 ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa
    11761 atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc
    11821 gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa
    11881 atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa cgcatctttg
    11941 gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg
    12001 atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg
    12061 ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat
    12121 gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct
    12181 tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc
    12241 ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga
    12301 acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt
    12361 gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc
    12421 cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt
    12481 gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa
    12541 gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt
    12601 gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact
    12661 gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt
    12721 cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa
    12781 gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt
    12841 gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga
    12901 aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt
    12961 taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga
    13021 tt
//
LOCUS       DAYYKC010000077        12929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000077 DAYYKC010000000
VERSION     DAYYKC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.078"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHBBW_002184"
     CDS             complement(56..1561)
                     /locus_tag="ACHBBW_002184"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9153845.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHBBW_002185"
     CDS             complement(1653..2243)
                     /locus_tag="ACHBBW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9153846.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHBBW_002186"
     CDS             2422..4068
                     /locus_tag="ACHBBW_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9153847.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHBBW_002187"
     CDS             4117..4836
                     /locus_tag="ACHBBW_002187"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9153848.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="ACHBBW_002188"
     CDS             5315..6262
                     /locus_tag="ACHBBW_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153849.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="ACHBBW_002189"
     CDS             6274..7194
                     /locus_tag="ACHBBW_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9153850.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="ACHBBW_002190"
     CDS             7235..8692
                     /locus_tag="ACHBBW_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9153851.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="ACHBBW_002191"
     CDS             9037..9651
                     /locus_tag="ACHBBW_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9153852.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="ACHBBW_002192"
     CDS             9644..11368
                     /locus_tag="ACHBBW_002192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9153853.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="ACHBBW_002193"
     CDS             11365..12816
                     /locus_tag="ACHBBW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9153854.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         4193 a         2237 c         2591 g         3908 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgg
//
LOCUS       DAYYKC010000078        12917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000078 DAYYKC010000000
VERSION     DAYYKC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.079"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..1446
                     /gene="efmA"
                     /locus_tag="ACHBBW_002194"
     CDS             160..1446
                     /gene="efmA"
                     /locus_tag="ACHBBW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9153855.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1584..2183)
                     /locus_tag="ACHBBW_002195"
     CDS             complement(1584..2183)
                     /locus_tag="ACHBBW_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9153856.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2587..3708
                     /gene="mnmA"
                     /locus_tag="ACHBBW_002196"
     CDS             2587..3708
                     /gene="mnmA"
                     /locus_tag="ACHBBW_002196"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9153857.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3954..5375
                     /gene="arcD"
                     /locus_tag="ACHBBW_002197"
     CDS             3954..5375
                     /gene="arcD"
                     /locus_tag="ACHBBW_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9153858.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5389..6507
                     /locus_tag="ACHBBW_002198"
     CDS             5389..6507
                     /locus_tag="ACHBBW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9153859.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6567..7859)
                     /locus_tag="ACHBBW_002199"
     CDS             complement(6567..7859)
                     /locus_tag="ACHBBW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9153860.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8009..8569
                     /locus_tag="ACHBBW_002200"
     CDS             8009..8569
                     /locus_tag="ACHBBW_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9153861.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8698..9075
                     /locus_tag="ACHBBW_002201"
     CDS             8698..9075
                     /locus_tag="ACHBBW_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9153862.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9008..11080
                     /locus_tag="ACHBBW_002202"
     CDS             9008..11080
                     /locus_tag="ACHBBW_002202"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9153863.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11291..12238)
                     /locus_tag="ACHBBW_002203"
     CDS             complement(11291..12238)
                     /locus_tag="ACHBBW_002203"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9153864.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12484..>12917
                     /locus_tag="ACHBBW_002204"
     CDS             12484..>12917
                     /locus_tag="ACHBBW_002204"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9153865.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4162 a         2340 c         2751 g         3664 t
ORIGIN      
        1 tgttattttt aatattttgc tgatttattg tttctaagta aagaaattta aattgaaacg
       61 attgttattt agggaaaaag gtattttttg aaaaaagaat cccgtataat gattgttgtg
      121 caaacgctat acaaaatgaa aaagaaaaga gggatgttta tggaaaatga acagtcggtc
      181 gttttaacga attggaagag aaattatttg ttctttttat cgggacagtt tttgtcagga
      241 atcactagta tggtcgtcca atatgcgatc atctggtatt tgacaagaga aaccggttcg
      301 gcgacaattt tgagttttgc tactttacta ggaatgattc ccatggtttt actaagccca
      361 tttgtaggac cgctagtgga tcgctgggat aaaaaagctc ttttgattgt cacggatatt
      421 attgtagcga ttttcgcgct tattttagca gtagtcggaa cgatttcaga atccttcccg
      481 atttggctag tttttgtgtc tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca
      541 acgatccagt cgattatgcc gacaatcgtt ccatcttctc acataacaag gacgaatgga
      601 cagttaggaa tggtccaatc agcgaatttc atcattgcgc cagctctagg tgcggctttg
      661 ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg tattgggggc agtttttggt
      721 gtcggcttat tgatttttgt gaagatccca aaagtttctc ctgaaatatt ggaagttccg
      781 cttactattt ttaaagatgc aaaatttggg ctgcagcaat tgatggataa taaagggtta
      841 tggtacataa cgatcaatgg tgcatttgtg atgctgttgt tcatgccagc aatcagtctg
      901 tatccattga tgacgctaga ttactttggt ggttccgttg gacaggcggg agcagtggaa
      961 gtagtctacg cagtcggaat gctcttaggc ggtgcactga tcagttttat aggaacatgg
     1021 aaagaccgaa tgaaacctat tattatagcc tacatcatta tgggcctgac gatcggtgca
     1081 agcggattgg ttccaaacga tagtcaaggg ttcttgtact ttcttatctt gaatgcagga
     1141 gcaggttgtg caacgccata tttcaataca ctgctgatgg caatgatcca gcagagttat
     1201 gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact ctttgatgaa cctggcaggt
     1261 ccaattggat tattatttgc aggaccatta gctgatcgtt taggggtaga aaaaatgttc
     1321 ttgttttccg gaatcgggat tttattatgc ggaatcgttt tatttttaac gtcagctgcc
     1381 cgaaattatg ataaagaatt acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac
     1441 gaatagtatg gaatggagtt gtgacagaag tcttgttaca actccatttc tgatcctaat
     1501 gttcctgtgg caataagcct actcaaatga aaaatatgag taattatctt tatttgttta
     1561 atacttgata tagggcagtt ttttcacgac ctatcgtact tcctctttgc ttcatagatt
     1621 tctttgattt gttgctcaga taacggagtg ccaaatggta gtttttcctt taagagttct
     1681 ctttccggtg cgtctacgcc tacattgaca tcgttctcga taggtacgct gtaatgatgg
     1741 atatgtccat gaagattgcg gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc
     1801 aagtaatatt gttgatggtc gaatttaacg atggcaccta catcataaaa ataaaatttg
     1861 ggacttccgt cagtaagcct aggatcattt ttttctaaat aacggaaaaa agcgcgactg
     1921 tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt
     1981 tctgcatttg ctttttcata ttgcggatgc atagcaaggt cgcctagatg ataaacatgg
     2041 tcggtttctt ttattactgc attccagcta gtgatcaact gttggttcat ttcatccaca
     2101 tttgcaaaga gccgcggagc aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc
     2161 atatcactga taaagtaatt catgtatctt ttgcctccct tttttcttag agtaaacaaa
     2221 atcacttatc tgagcaactc ataacatcta aaaagaaaag gttgtaaatg agacgtgcag
     2281 ctttaagcga taagaagcaa ttttgaaaaa tagtttttca caatttataa aattattcct
     2341 tattgccgaa aggctggccc tttttatgag tatgccaatt ttttatatcg aatgctcaaa
     2401 agatgagtga ttttttggtt gactatcatg tttgtgtact gaataggtcc agtagaacag
     2461 tatttttacg acttgataat taagggatga aattttcaga aatatgacgt ataatgtaaa
     2521 atactgaaaa gttgcgacct tcaactcgta gatgtttcag aatctaattg aaatgatggt
     2581 gatatcatga cagacaacag caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac
     2641 tcatctgtaa cagcactttt gttaaaggaa caaggatacg acgtgatcgg aatattcatg
     2701 aaaaattggg atgacacgga cgaaaacggt gtatgcacgg ccacggaaga ttataaggat
     2761 gtagcgaaag tagctgctca aatcgggatt ccttattact ctgtgaattt tgaaaaagaa
     2821 tattgggatc gtgtatttga atatttccta gccgaatacc gcgcaggacg tacacctaac
     2881 ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat tcttggatta tgcaatggat
     2941 cttggcgccg attatgtagc tactggacat tatgcgcaag tcactcgcga tgaaaacggc
     3001 gtggtccaca tgcttcgtgg agtagacaat aataaagatc aaacttactt ccttagccaa
     3061 ttatcacaag aacagctttc aaaaacgatg ttccctctag gcggcatgga aaaatcagaa
     3121 gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga
     3181 atctgcttta ttggtgaaaa gaacttcaaa caatttttga gcaactatct gccagcaaaa
     3241 aaaggaaata tggtcactct tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat
     3301 tatacgattg gtcaacgtca aggactaggg atcggtggcg gaggagattc gcaagaaccc
     3361 tggtttgttg taggaaaaga tctagcgaca aacacattat atgtggggca agggttccat
     3421 catcctgcat tgtatgccac aagcttggat gcaagtgaaa tccactttac gacgaatgaa
     3481 ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt atcgtcagca agacgttcct
     3541 gtgacagttc gcttattgga tgataatcgt gcagaagtcg tattcgacga acctgtacgt
     3601 gcaatcactc ctggacaagc agttgtattt tatgatggaa tggaatgtct gggtggtggt
     3661 ttgatcgacc acgcatatca agaaacaaaa gttttacaat acgtttaagc gatattaaat
     3721 gaatcaaaac agctatggct aacaaaagaa acaccttttc ttttggatag catagctgtt
     3781 tttttgcaat aaaattaatt tttattcatt ttatacttga aatacttgtg taaaaagttc
     3841 ataatatcca ataatatgaa taaagttgct ttttgttaag caagagcagt tgcaaaatag
     3901 aagggaatct tttgcaagtg atttgttcaa ttttataagt gagggtgatg gaaatggaaa
     3961 agaaatcgac gggtattagc aaatggggac tggttgctct ggttgtcagt tcgtcaatcg
     4021 gaagcggtgt gtttgggatc acaagtgatc tggctagttc tgcagctcca gggccagcga
     4081 tcctttcatg gataatcgtt gggattggta ttttagcttt ggtattatcg ctgaatcatt
     4141 taggagaaaa gcgtccagat ctagatggtg gtattttcgg ttacgcggaa gcttcatttg
     4201 gtaaattagg tggcttcatc agtggttggg gttactggtt gtctgcgtgg ctgggaaatg
     4261 ttgcttttgc aacgatgctg atgagcgcca tgggagaatt tttcccgata ttcaaaggtg
     4321 gacaaaatgt tccttctatt ttatttgcaa gtatttttat ttggggtctg actttattag
     4381 taaacaatgg agttgaagga gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag
     4441 tgccattatt cctattttta atatttgtat ttatcgcatt taaagtgaat atattcactg
     4501 ctgatttttg gggtaacgta tctgataatc tagtgaatgg aacagggcag caaggaacga
     4561 tctggttgca gattaaaggt tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag
     4621 gcgcaagtgt tttagccaac cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca
     4681 ttggtctgct ttgtttattg tttatctata tccttgcttc gatcttgcca tacggcgtct
     4741 tatctcaaga agagttagca actatttcac aacctgctat ggcaaatatt ttaaaaggaa
     4801 tcgtgggaaa ttggggagca gcgctgatca atatcggctt gattatttcg atcattggtt
     4861 cttggttgtc ttggacgatg ctcccagcag aaacaacgat gttgatggct cgagacggtc
     4921 tgttgccgaa aggttgggga aaaacgaata aaaaaaatgc cccaacttat tcgttagttg
     4981 ttactgcaat cttgactaat ctattcttat tgactttctt agttactgat tatgcctatc
     5041 aatttgccta ttctttatgt acagcggcaa ttttgatttg ttatctactt gtcgggatct
     5101 atcaagtcca gttttcttta aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg
     5161 ctgtagtttt tgaattgatg ggcatcattt tagcaggatt ttcttatgtc cttttatgta
     5221 gtattgccta tcttcctggt ttttgcttct actggatcgc ttgtcgtgaa accaaacatc
     5281 aaattacggg aaaagaaaaa gtaatgattg ggctgatttg tgtggccgca ctgctttcaa
     5341 tcgggttgct tgctgtaggc tggatcaaga tataaggggg aaagaaacat gaaaatcgca
     5401 aatttcggtg tagaagaatg gctaaataaa tgggaaaaga aagcggtata tgatattgca
     5461 caaagctcga tcgaagcatt gacattagac gaactcgttg gcttagatgg gactagcgta
     5521 agtaaatttt tcgaaaaacg caaaaacgaa ccattggact atggctggat cgaaggatca
     5581 gatgttttca aacaagaagt cgcttctctt tatcaaaccg tcgatccgga aaacatctta
     5641 caaacgaatg gagcgactgg tgctaatctt ttagctctat acgcgttagt ggaagcagga
     5701 gaccatgttg tttcgatgct tcctacttat cagcaattat atgacatccc caaatcttta
     5761 ggtgccgctg ttgactttgt tcatttgaag gaagaagaag attggcagtt tgatttagaa
     5821 cagctagaag ccttagtcaa acctgaaaca aaaatcatct gtttgaatag tgcaaacaat
     5881 cctacaggca cattgctaga tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca
     5941 gtagatgcat atgtattgat agatgaagta tatgcgcctt taacggataa aggtgaattc
     6001 ttgtcgatcg tagatgtcta tgaaaaaggt atagcaacca attccctatc caaaacctac
     6061 tccattccag gtattcgtat tggctggaca gctactggac ctgagttggc agaagttttt
     6121 cgaaagtatc gagactacac gatgatttgc ggcggagtcc tgtcagatga tcttgctgta
     6181 catgcattga agaataaaga aaaaatattg gcgcgaaaca aaaagatcat tacagagaac
     6241 cttgtgattt taaaacaatg ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat
     6301 tacgtctcta cttctttcat taaattagcc atcgaagaag atgaccagac attttgtatc
     6361 aatttattag aagaaacagg agtactatta gtaccaggaa gtgcctttga tttaccgaag
     6421 catgcccgac taggttactg ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta
     6481 tctgagttct tgaaaaaaca ggcctaaaca aaaacactgc aaggacagtc tgtgacaact
     6541 gttcaagcag tgtttttcat ttacgcttat tgtctatgag aaggagagtg ccctgaagct
     6601 atggagactc ctgaagcttt ttcttctgca gtttcttgct gatggatctc ttgtgtattg
     6661 tgtcgtacaa gcaagaagtt gaccaaaaca agaagagaag tcgaaagaaa gacgccgcga
     6721 taaccaaatg cactggaaac actggagccg atcataggcc ctactacgtt accagttgct
     6781 tggaaagatt ggttataact gaagatccgt ccagcagctt gatgaggcga atattttgtg
     6841 atcaatgctt ggacagcagg gagcagacaa gcatcagaga taccaatcaa aaaacgtaag
     6901 gcagctaatt gccaaacatt cgtgacgaat gccattggga tataaaccag aatcgcaaaa
     6961 cccaaaccaa tcgcaagtat tctttcactc ccaatcttgt cacctaagcg accaaatctt
     7021 ggagcggcta tcaaagtggc aatcccaggt atcgaagcaa tgattccgct gatcaacgta
     7081 acattcccat gtccatgcag caattggcga atgtacagac tgatgattgg actaatggaa
     7141 ttgttggatg cttggatgat catcgttgtg acgaacatac cgatgacgac gtgtggatat
     7201 ttcaagtctt tgaaaatctg tttggcagaa accatgtttt cttttttcac tgggacaaat
     7261 ttttcatgga cgaaaaacag actcaacaag aagacgaaaa ataagatgat ccctgtaatg
     7321 aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc cgccaagaag cggtccaagt
     7381 aatgttcctg taacagaacc agttgctaag gttccaagaa cttgaccact tttttctttt
     7441 ggcgtcccag ttgcgacaag ggcagtggca ttactgatat aaccagaaaa aatcccttga
     7501 agcagacgca atgcgactaa ttgatacaca ctagtcacgc aacccatcaa agaaatgacg
     7561 attgccattc ctaatgaagc acgcaacagc atcaattttc ttcctttttg gtcggctaat
     7621 tttccccacc aaggagagat aagcgtagtc accaagaaag tagatgaaaa agtcaatccg
     7681 ctccaaaagt tcaattgtga cgtactataa ttacctaacg tatctatgta aagtgacata
     7741 aaaggcatga ctaaactaaa tccgatacca gccataaatg ttccaaacca taagacaaac
     7801 aagttttgtt cccaagtttc tcttttccta aataattttt cctttattct tgcagccaat
     7861 tagactcctt ctttctgaaa attttcatct acagctcttt attataaact acaatatgta
     7921 gtttatcaat gaaagtctct tttttctctt tctttttcgt ggtaaactga aagagtgaag
     7981 gatttatttt atgagaggaa acatggaaat gaaaaaaaga aatctgtctc aagagaaaat
     8041 catcgactgc tttcgagaac tagcagaaga gatggatgtt cagcaaataa cctttcagca
     8101 tttagccaaa cgcttggata tcaaggctcc ctcgctgtat aaccatttca aaaatattcg
     8161 tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa cttaatgacc agctgcgacg
     8221 ggctcttgtg ggaaaaagtg gtcaggaagc actgcagatc tatgcagaaa cttatcgatc
     8281 ctttgctttt tcaaatcaag cagtgtatga gttactgatc agtgtaccgc ataccaatga
     8341 agctgtttta ttagatggaa tccatgaaac gaatcagatc attttgcaga tatttgaggc
     8401 ttttcaatta acgaaagaag aaaaagttca tcgaagcagg gaacttcgta gtatgatcca
     8461 cggttatctt tccttacgtt ttttaggata ctttacaaaa gaaccaacaa tcgatccaga
     8521 agacagctat ttttggatga tcaataactt tatcgctact ttgccttaaa caatcagatt
     8581 catttgtaac aattttaaga tgttcattaa atgacccttt tataagagaa aattcttgca
     8641 actttcttga aaagtgacta agatgagtct agaaaagctg aaagaaggag cacgagtatg
     8701 tatcaagtaa ttaccatgtt cggggacaat gagccttggt ggttttttga agactgggaa
     8761 gaagatattg aagaagaaga aacattcgag tctttcgaag aagcacgaca agcgtacgaa
     8821 aaacaatgga acgaaatcca tcaaaattat gaatatatca atgcaaaaag taactttctc
     8881 agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag agtgtgacga tgatttacag
     8941 caatacaagg gacttgcgtt gttgaaaaat catcagccca tcacagtaga aagcagaaag
     9001 gaattttatg aaacaactaa ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa
     9061 ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa ggacccaagg tgatcgttac
     9121 ttgggccctt ggtcatttat taggattgaa aatgccggaa gatctgaata aggaatggca
     9181 gagctggcaa atggacacgt tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc
     9241 taaaacagga catcaattaa aagcaatcaa gcagctggct cagcgaaaag atgtctcaga
     9301 agcagtcatt gcgacagatg ccggaagaga aggggaactt gttgctcgct ggatcttaga
     9361 gtatgtgcat ttcaataaac cagtgaagcg gttatggatc tcttctcaaa cagataaagc
     9421 aatcaaaact ggattcaagc aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc
     9481 tgctcttgcc cgggcaaaag cggactggct ggtaggattg aatgtgacca gagccttaac
     9541 tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag acaccgacac tagcattagt
     9601 gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt
     9661 gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca
     9721 agaagaagca caaaaacttg ttcaattgat cagtacaagc aaaggaactg tcacagaagt
     9781 ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat gatttaacag agatccaaag
     9841 agaagccaat caacgctatg gctattctgc aaagaaaaca ttaagtcttg tacaaagttt
     9901 gtatgagacc cataagatcg ttacttatcc gcgtacagac agcaaatatc tgacaaatga
     9961 catgaaaagt acgatgaaag aacgattgca agcagtcagc gactttgctc ctgaggtcaa
    10021 gcagtatttg aaaaacggag ccatcgtcca acagacaaaa gtattccaag acagtaaagt
    10081 gaccgatcac catgcacttt tgccaactga aaacagagca cgttatgaaa aattaagtaa
    10141 tgaagagcga aaaatctatc aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc
    10201 ccataaagtc agccaaacaa aagttacggt tgaatttggt aaagaacgat tcatttttcg
    10261 gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact gaaactgaga cggaaacagt
    10321 caaatgggaa aaaggaatga gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc
    10381 accgcctaaa ccattgtcag aagcaagctt gcttggtcaa atggaaaaat acagtctggg
    10441 aaccccagct acgcgtgcag agattatcga gaaactaatc aaatctgaac tgatggaacg
    10501 tacccctcaa ggactgcaag tatctcctaa aggtaagcaa ttattagtat tggtaaatcc
    10561 ctctttagta acaccagatt tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc
    10621 aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt
    10681 gaatgagatc aaatcaagca aacaagaata tcaagatttc tcgattaccc aaaagaaatg
    10741 cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg
    10801 caccaattca gattgcaact accgcagaag aaaagatcca aaagtctcta atcatcgatg
    10861 cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa aacggtgctt atttccgttg
    10921 taaatacgat ggaacgacag aaaaattgct tgataaaaaa gagcagaaga agaaaatgac
    10981 caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag
    11041 cccattagca gcagcactaa aagcagcaat gaagaagtaa taagatatga aagaggttgt
    11101 gatagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt
    11161 ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg aacaccattt attcggaaaa
    11221 aaggactgtg acaagacttt tgtcacagtc ctttttcatt taaaaaaatg tttacttcat
    11281 ttgacgtata ttattggtga ggcttcaatt catttggctt cactttttct gtttctctta
    11341 tgatataaac aggacgctgt ttcgtttcta agaagatttt cccaatatat ttcccgatga
    11401 ttccaagaca taacaattgg attccaccaa tgaacaggaa gatcgagacc atggaaggcc
    11461 atcctgatgt aggatcacca aacaacaatg cccgcaaaat aatgaacaaa attgcaatac
    11521 cagaaccgac acaagacaaa gcgccgataa atgaagcaat cgttaacggt gcatccgaga
    11581 agttcacgat accatcaatc gagtagttga ataatttcca gaaggaccaa gatgtttcgc
    11641 cagccacacg ttcgcggttt tcataagaaa tatattctgt tttaaatccg acccagctaa
    11701 ataatccttt tgaaaatcgg ttgtattctg ttaattctag gacggcatcc accatttgac
    11761 gagtcatcaa gcggaaatca cgagcgccat cgaccatttc tgtatcgctg attttattga
    11821 tcaactggta gaattttttc gcaaagaaac tgcgtatcgc aggctcgcct tcacgagtcg
    11881 aacgccgggt gccgacacaa tcgatatcag aagtagtgat ccggttgatc atctcaggaa
    11941 gcagttcggg cggatcttgc agatcaacat ccatcacggt cactaaatct cctgtagaag
    12001 cttgcaatcc agcgtacaga gccgcttctt tcccaaaatt tctggaaaat gagacaaagc
    12061 ggacataagg ataagctgag gctagcttct tgattgctgc taacgtaccg tcttttgatc
    12121 cgtcgtcaat aaacaaatat tcgaactcat gggcaccttt gaatttttca acctcatcaa
    12181 aaaaaagagg aacagttttt tcttcattaa aacatgggac aacaattgat actttcatcg
    12241 tttctatcct ttcaatcgta tgtttatata tggtttacat tcaaaattta ataaacctta
    12301 aataacacct ttaaaatttg attttacgta aaagaacagg tattcttttt gtaaactaac
    12361 gtatttagtt taccatgttt cctcggttta tcaaactttt aagaacaatt tttattagtt
    12421 ggcaagtagt tttggataga gaaaaaggag gaagttccag tctattgact aggagcaaac
    12481 aaaatgaaaa tacacacacc tatcggcttt ttatggttag atgcagatga atccggattg
    12541 actgctgtat cttttcagcc aatttcagaa agcgaatcag cgaatcagcg aattttaacg
    12601 cgcacacaag aacagtttga agaatatttt gcgggaaaaa gaaaagaatt tgatgttcct
    12661 ttatctataa agagaggtac gccttttcag caaaatgtct ggaaagcttt gtcgaccatt
    12721 ccttatggag aaatccgaac gtataaagaa gtagccgttg ctgttgatag tccgaaagct
    12781 gtgcgagcga ttgggcaggc aaatcgaacc aatccattgc caatcgttat tccttgtcat
    12841 cgagtgatcg ggcaaaatgg aaaactgaca ggatatatgg gaaacgcaat agatggggtt
    12901 gaaatcaaac gccaatt
//
LOCUS       DAYYKC010000079        12882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000079 DAYYKC010000000
VERSION     DAYYKC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.080"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..753)
                     /locus_tag="ACHBBW_002205"
     CDS             complement(109..753)
                     /locus_tag="ACHBBW_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9153866.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(847..981)
                     /locus_tag="ACHBBW_002206"
     CDS             complement(847..981)
                     /locus_tag="ACHBBW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9153867.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHBBW_002207"
     CDS             complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHBBW_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9153868.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(3112..3585)
                     /locus_tag="ACHBBW_002208"
     CDS             complement(3112..3585)
                     /locus_tag="ACHBBW_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9153869.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHBBW_002209"
     CDS             3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHBBW_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9153870.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHBBW_002210"
     CDS             4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHBBW_002210"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9153871.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHBBW_002211"
     CDS             6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHBBW_002211"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9153872.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(7632..8771)
                     /locus_tag="ACHBBW_002212"
     CDS             complement(7632..8771)
                     /locus_tag="ACHBBW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9153873.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(9080..9793)
                     /locus_tag="ACHBBW_002213"
     CDS             complement(9080..9793)
                     /locus_tag="ACHBBW_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9153874.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHBBW_002214"
     CDS             complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHBBW_002214"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9153875.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            10982..12514
                     /locus_tag="ACHBBW_002215"
     CDS             10982..12514
                     /locus_tag="ACHBBW_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9153876.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(12705..>12882)
                     /locus_tag="ACHBBW_002216"
     CDS             complement(12705..>12882)
                     /locus_tag="ACHBBW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9153877.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4125 a         2605 c         2272 g         3880 t
ORIGIN      
        1 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
       61 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
      121 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
      181 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
      241 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
      301 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
      361 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
      421 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
      481 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
      541 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
      601 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
      661 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
      721 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
      781 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
      841 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
      901 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
      961 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     1021 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     1081 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     1141 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     1201 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     1261 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     1321 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     1381 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     1441 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     1501 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     1561 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     1621 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     1681 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     1741 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     1801 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     1861 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     1921 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     1981 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     2041 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     2101 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     2161 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     2221 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     2281 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     2341 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     2401 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     2461 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     2521 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     2581 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     2641 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     2701 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     2761 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     2821 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     2881 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     2941 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     3001 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     3061 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     3121 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     3181 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     3241 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     3301 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     3361 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     3421 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     3481 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     3541 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     3601 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     3661 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     3721 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     3781 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     3841 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     3901 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     3961 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     4021 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     4081 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     4141 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     4201 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     4261 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     4321 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     4381 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     4441 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     4501 ccaaatacga tgacttttcc atctattatg gaaaaaatca attacttgat cgatcaagaa
     4561 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     4621 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     4681 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     4741 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     4801 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     4861 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     4921 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     4981 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     5041 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     5101 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     5161 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     5221 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     5281 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     5341 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     5401 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     5461 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     5521 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     5581 acagatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     5641 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     5701 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     5761 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     5821 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     5881 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     5941 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     6001 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     6061 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     6121 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     6181 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
     6241 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaacg
     6301 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
     6361 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
     6421 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
     6481 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
     6541 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
     6601 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
     6661 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
     6721 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
     6781 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
     6841 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
     6901 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
     6961 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
     7021 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
     7081 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
     7141 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
     7201 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
     7261 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
     7321 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
     7381 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
     7441 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
     7501 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
     7561 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
     7621 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
     7681 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
     7741 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
     7801 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
     7861 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
     7921 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
     7981 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
     8041 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
     8101 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
     8161 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
     8221 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
     8281 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
     8341 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
     8401 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
     8461 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
     8521 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
     8581 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
     8641 attgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
     8701 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
     8761 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
     8821 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
     8881 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
     8941 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
     9001 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
     9061 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
     9121 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
     9181 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
     9241 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
     9301 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
     9361 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
     9421 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
     9481 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
     9541 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
     9601 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
     9661 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
     9721 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
     9781 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
     9841 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
     9901 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
     9961 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    10021 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    10081 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    10141 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    10201 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    10261 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    10321 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    10381 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    10441 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    10501 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    10561 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    10621 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    10681 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    10741 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    10801 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    10861 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    10921 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    10981 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    11041 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    11101 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    11161 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    11221 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    11281 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    11341 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    11401 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    11461 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    11521 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    11581 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    11641 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    11701 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    11761 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    11821 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    11881 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    11941 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    12001 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    12061 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    12121 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    12181 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    12241 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    12301 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    12361 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    12421 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    12481 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    12541 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    12601 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    12661 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    12721 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    12781 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    12841 gaaccagccg cacctccaat aaagtacata aacataaata ca
//
LOCUS       DAYYKC010000080        12857 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000080 DAYYKC010000000
VERSION     DAYYKC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.081"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1938
                     /locus_tag="ACHBBW_002217"
     CDS             55..1938
                     /locus_tag="ACHBBW_002217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9153878.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2013..2297)
                     /locus_tag="ACHBBW_002218"
     CDS             complement(2013..2297)
                     /locus_tag="ACHBBW_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9153879.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2287..3021)
                     /locus_tag="ACHBBW_002219"
     CDS             complement(2287..3021)
                     /locus_tag="ACHBBW_002219"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9153880.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3150..3773
                     /locus_tag="ACHBBW_002220"
     CDS             3150..3773
                     /locus_tag="ACHBBW_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9153881.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3965..5086
                     /locus_tag="ACHBBW_002221"
     CDS             3965..5086
                     /locus_tag="ACHBBW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9153882.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5093..8221
                     /locus_tag="ACHBBW_002222"
     CDS             5093..8221
                     /locus_tag="ACHBBW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9153883.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8240..11434
                     /locus_tag="ACHBBW_002223"
     CDS             8240..11434
                     /locus_tag="ACHBBW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9153884.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11580..12287)
                     /locus_tag="ACHBBW_002224"
     CDS             complement(11580..12287)
                     /locus_tag="ACHBBW_002224"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9153885.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4405 a         2241 c         2678 g         3533 t
ORIGIN      
        1 cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa
       61 aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta
      121 gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg
      181 ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa
      241 tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc
      301 tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga
      361 gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg
      421 tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg
      481 catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta
      541 ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc
      601 ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt
      661 tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt
      721 tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc
      781 gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac
      841 aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca
      901 gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg
      961 attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa
     1021 gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg
     1081 attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat
     1141 tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc
     1201 aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg
     1261 gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt
     1321 aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa
     1381 gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa
     1441 ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat
     1501 ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt
     1561 gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat
     1621 ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta
     1681 tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt
     1741 gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat
     1801 gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat
     1861 tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag
     1921 acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg
     1981 aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt
     2041 gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct
     2101 gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt
     2161 cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta
     2221 gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa
     2281 taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt
     2341 gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc
     2401 aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg
     2461 tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac
     2521 catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa
     2581 cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt
     2641 tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac
     2701 gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt
     2761 aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat
     2821 agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc
     2881 aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga
     2941 aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata attggtcgat
     3001 tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta
     3061 tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact
     3121 atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc
     3181 gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc
     3241 aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg
     3301 cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc
     3361 cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag
     3421 gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca
     3481 tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca
     3541 gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc
     3601 tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca
     3661 agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca
     3721 aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata
     3781 agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg
     3841 aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag
     3901 acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga
     3961 ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga
     4021 cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa
     4081 agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc
     4141 cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt
     4201 tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag
     4261 ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa
     4321 aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga
     4381 agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga
     4441 gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt
     4501 aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact
     4561 acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca
     4621 ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa
     4681 aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga
     4741 acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt
     4801 ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat
     4861 cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc
     4921 aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca
     4981 aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga
     5041 aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc
     5101 gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg
     5161 tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat
     5221 ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa
     5281 agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga
     5341 acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa
     5401 aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg
     5461 aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat
     5521 gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa
     5581 ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca
     5641 atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt
     5701 ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga
     5761 ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact
     5821 cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca
     5881 actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat
     5941 attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg
     6001 atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga
     6061 aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc
     6121 gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca
     6181 aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt
     6241 cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga
     6301 agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat
     6361 tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca
     6421 gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg
     6481 gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag
     6541 tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca
     6601 gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc
     6661 aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa
     6721 agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc
     6781 caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat
     6841 catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa
     6901 ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc
     6961 tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca
     7021 acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa
     7081 taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac
     7141 ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc
     7201 gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct
     7261 tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa
     7321 ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga
     7381 aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc
     7441 ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct
     7501 aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat
     7561 gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa
     7621 aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac
     7681 gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga
     7741 acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca
     7801 acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg
     7861 gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca
     7921 taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga
     7981 tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt
     8041 tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag
     8101 tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca
     8161 acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata
     8221 aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg
     8281 tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg
     8341 gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga
     8401 gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc
     8461 tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat
     8521 gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct
     8581 aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac
     8641 gattgagact aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg
     8701 ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc
     8761 ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg
     8821 ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca
     8881 tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct
     8941 tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca
     9001 tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt
     9061 atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga
     9121 ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa
     9181 atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga
     9241 agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc
     9301 agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt
     9361 cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt
     9421 ctatggcgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt
     9481 tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg
     9541 ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc
     9601 ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa
     9661 attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac
     9721 cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca
     9781 tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct
     9841 ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc
     9901 gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa
     9961 tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct
    10021 cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga
    10081 ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat
    10141 tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga
    10201 aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa
    10261 ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa
    10321 taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc
    10381 cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga
    10441 tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc
    10501 tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg
    10561 gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact
    10621 tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac
    10681 tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga
    10741 acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga
    10801 ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca
    10861 aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga
    10921 acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc
    10981 tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac
    11041 attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa
    11101 tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct
    11161 cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca
    11221 agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat
    11281 ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt
    11341 ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg
    11401 catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg
    11461 ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc
    11521 tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt
    11581 tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg
    11641 caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta
    11701 gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct
    11761 ggaagcatat gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa
    11821 cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc
    11881 ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc
    11941 ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt
    12001 tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct
    12061 aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct
    12121 gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc
    12181 atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc
    12241 tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc
    12301 tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta
    12361 aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt
    12421 ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca
    12481 aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc
    12541 gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat
    12601 ttgatttatt tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt
    12661 atataacaga gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac
    12721 attactatta aagaaataaa aaacataaaa aatcttaaga aatcggtttc atttagcttt
    12781 tgagataaaa taatagtaca gttttatacg ctgtttcata tgtttagaaa atttgaatta
    12841 gatttgaaaa ctctaat
//
LOCUS       DAYYKC010000081        12188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000081 DAYYKC010000000
VERSION     DAYYKC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.082"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..314)
                     /locus_tag="ACHBBW_002225"
     CDS             complement(150..314)
                     /locus_tag="ACHBBW_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153886.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            609..1937
                     /locus_tag="ACHBBW_002226"
     CDS             609..1937
                     /locus_tag="ACHBBW_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9153887.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2139..2972
                     /locus_tag="ACHBBW_002227"
     CDS             2139..2972
                     /locus_tag="ACHBBW_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9153888.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2988..3725
                     /locus_tag="ACHBBW_002228"
     CDS             2988..3725
                     /locus_tag="ACHBBW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9153889.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3880..4848)
                     /locus_tag="ACHBBW_002229"
     CDS             complement(3880..4848)
                     /locus_tag="ACHBBW_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9153890.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5080..5913
                     /locus_tag="ACHBBW_002230"
     CDS             5080..5913
                     /locus_tag="ACHBBW_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9153891.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5947..6786
                     /locus_tag="ACHBBW_002231"
     CDS             5947..6786
                     /locus_tag="ACHBBW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9153892.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6812..7372
                     /locus_tag="ACHBBW_002232"
     CDS             6812..7372
                     /locus_tag="ACHBBW_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9153893.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7571..9292
                     /locus_tag="ACHBBW_002233"
     CDS             7571..9292
                     /locus_tag="ACHBBW_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9153894.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9457..10599
                     /locus_tag="ACHBBW_002234"
     CDS             9457..10599
                     /locus_tag="ACHBBW_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9153895.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10615..11826
                     /gene="thiI"
                     /locus_tag="ACHBBW_002235"
     CDS             10615..11826
                     /gene="thiI"
                     /locus_tag="ACHBBW_002235"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9153896.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2046 c         2531 g         3458 t
ORIGIN      
        1 ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct
       61 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
      121 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
      181 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
      241 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
      301 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
      361 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
      421 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
      481 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
      541 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
      601 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
      661 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
      721 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
      781 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
      841 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
      901 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
      961 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
     1021 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     1081 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     1141 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     1201 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     1261 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     1321 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     1381 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     1441 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     1501 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     1561 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     1621 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     1681 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     1741 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     1801 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     1861 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     1921 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     1981 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     2041 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     2101 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     2161 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     2221 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     2281 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     2341 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     2401 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     2461 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     2521 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     2581 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     2641 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     2701 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     2761 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     2821 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     2881 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     2941 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     3001 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     3061 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     3121 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     3181 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     3241 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     3301 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     3361 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     3421 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     3481 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     3541 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     3601 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     3661 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     3721 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     3781 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     3841 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     3901 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     3961 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     4021 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     4081 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     4141 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     4201 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     4261 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     4321 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     4381 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     4441 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     4501 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     4561 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     4621 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     4681 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     4741 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     4801 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     4861 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     4921 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     4981 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     5041 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     5101 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     5161 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     5221 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     5281 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     5341 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     5401 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     5461 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     5521 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     5581 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     5641 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     5701 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     5761 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     5821 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     5881 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     5941 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     6001 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     6061 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     6121 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     6181 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     6241 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     6301 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     6361 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     6421 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     6481 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     6541 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     6601 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     6661 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     6721 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     6781 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     6841 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     6901 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     6961 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     7021 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     7081 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     7141 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     7201 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     7261 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     7321 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     7381 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     7441 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     7501 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     7561 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     7621 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     7681 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     7741 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     7801 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     7861 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     7921 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     7981 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     8041 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     8101 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     8161 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     8221 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     8281 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     8341 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     8401 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     8461 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     8521 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     8581 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     8641 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     8701 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     8761 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     8821 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     8881 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     8941 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     9001 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     9061 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     9121 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     9181 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     9241 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     9301 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     9361 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     9421 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     9481 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     9541 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     9601 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     9661 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     9721 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     9781 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     9841 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     9901 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     9961 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
    10021 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    10081 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    10141 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    10201 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    10261 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    10321 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    10381 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    10441 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    10501 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    10561 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    10621 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    10681 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    10741 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    10801 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    10861 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    10921 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    10981 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    11041 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    11101 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    11161 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    11221 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    11281 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    11341 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    11401 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    11461 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    11521 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    11581 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    11641 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    11701 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    11761 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    11821 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    11881 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    11941 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    12001 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    12061 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    12121 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    12181 agaggttg
//
LOCUS       DAYYKC010000082        12153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000082 DAYYKC010000000
VERSION     DAYYKC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.083"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(362..1087)
                     /locus_tag="ACHBBW_002236"
     CDS             complement(362..1087)
                     /locus_tag="ACHBBW_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153897.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1241..1567)
                     /locus_tag="ACHBBW_002237"
     CDS             complement(1241..1567)
                     /locus_tag="ACHBBW_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153898.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1604..1969)
                     /locus_tag="ACHBBW_002238"
     CDS             complement(1604..1969)
                     /locus_tag="ACHBBW_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9153899.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(2006..2221)
                     /locus_tag="ACHBBW_002239"
     CDS             complement(2006..2221)
                     /locus_tag="ACHBBW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153900.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2350..2685
                     /locus_tag="ACHBBW_002240"
     CDS             2350..2685
                     /locus_tag="ACHBBW_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153901.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2761..4758)
                     /locus_tag="ACHBBW_002241"
     CDS             complement(2761..4758)
                     /locus_tag="ACHBBW_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153902.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4856..5131)
                     /locus_tag="ACHBBW_002242"
     CDS             complement(4856..5131)
                     /locus_tag="ACHBBW_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153903.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5363..6097
                     /locus_tag="ACHBBW_002243"
     CDS             5363..6097
                     /locus_tag="ACHBBW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9153904.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6136..7278
                     /locus_tag="ACHBBW_002244"
     CDS             6136..7278
                     /locus_tag="ACHBBW_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153905.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7906..8391)
                     /locus_tag="ACHBBW_002245"
     CDS             complement(7906..8391)
                     /locus_tag="ACHBBW_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM9153906.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8393..9064)
                     /locus_tag="ACHBBW_002246"
     CDS             complement(8393..9064)
                     /locus_tag="ACHBBW_002246"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153907.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(9327..10520)
                     /locus_tag="ACHBBW_002247"
     CDS             complement(9327..10520)
                     /locus_tag="ACHBBW_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9153908.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10604..10804)
                     /locus_tag="ACHBBW_002248"
     CDS             complement(10604..10804)
                     /locus_tag="ACHBBW_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9153909.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(11228..11461)
                     /locus_tag="ACHBBW_002249"
     CDS             complement(11228..11461)
                     /locus_tag="ACHBBW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153910.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11468..11899)
                     /locus_tag="ACHBBW_002250"
     CDS             complement(11468..11899)
                     /locus_tag="ACHBBW_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9153911.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3730 a         2483 c         1954 g         3986 t
ORIGIN      
        1 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
       61 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
      121 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
      181 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
      241 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
      301 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
      361 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
      421 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatatct ttaccaactt
      481 tctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
      541 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagtt
      601 tccaagtagg tggtatagct gtgtatgttt ctacaaatga attaagtttt tcattaagtg
      661 acttctcttt tatatttcca tctaaaaact ctgtatatat ggcttgagct atactaaaac
      721 aaagctggcc ttgttctaat aattttacat cattagttag taaagctgtg ttcacgttat
      781 caatcgcttc aagaagagta tctttggtgt ccctaacagc tttaggtgtg ccttctggaa
      841 tttccctact tgatacctcg tccattttat cttccataaa ccccttaaaa tcatatcttt
      901 ctcccaaatc catagaagtt tttgagaaag ctttcatttc tgagtctatt tcttcaataa
      961 tatttaacat gtctgataca tattgtttag gctgattcgt ttttaatcta tatgtggtaa
     1021 atatctcaat tcctccaatt atcaataaaa ttgccaaaca aatccataat aattttttct
     1081 ttttcatttt tcctcctata cactgctgtt ataacagtga ctaatgtaaa gattttccca
     1141 gcaatcatca gactattatt tacagggaat caaaaaaact ccttagtaag cttacacctt
     1201 taggagttcc tgtttgaacg gtatgcataa tacatttaaa tcaatttaac attaacacct
     1261 cagtatatgg tatctctaca tatacacggt ctttttttgg atagtactcg acatttatat
     1321 tgatagcact tccattgtct aacgtagctt tagcccggta cgtgttatca ccattatcat
     1381 gagtgatcgt cacatcatca catgtccgat ttaagttata ctgttcttgt aaaatctgag
     1441 ttaccaatgg tacagatgac ttctctagtc cagatgaacc cgtcattctt gaaaaaatta
     1501 tacctataat agccaataca acgataccta taatgacaaa cttcttattc atatttactt
     1561 tattcaactt tttatctcct atctccaaaa tgattttctc tcatcagtaa ctcattgaca
     1621 tccccaatag tacaccaaag atggctccag ctacccctgc tatcatcaaa atcgtaccca
     1681 tttgttcatc tttctctcga tataagtaac ccataataac agcaacagca ccaaatacca
     1741 caggaataaa tgctaatgat actaccatag aaatccaacc aataatcaaa tacagcttcc
     1801 ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt
     1861 tctttactgt gtctgttccg gttgtctcat ctgtttcttg taatgctccg caaaactggc
     1921 aatacttact ttcatttgtt atttctttac cacattttct acaatacatg tcttctcctc
     1981 ctaaaatcat tcaattgctt attagctaac tgtttacttt attaaataca tcagaaaaca
     2041 gctcatttat tggtacactt aatatctcag aaagcatttt catttgatag ggtttaggaa
     2101 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt tttttggcta
     2161 actcagtttg ggaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
     2221 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
     2281 tataaatact tcaaatgtca ttacaaatac aacaacttgt tgtactctac tgagaagggg
     2341 ggtgtattaa tgaaacagaa gcaatatgca aaggtcatta gagaaaagcg aaaagcactc
     2401 gggtggacac aaaaagaatt agctaaaaaa atcttctcta cacaacaggc agtagcaaga
     2461 tgggaaaatt ctgtaacaga acctaacctc gatagcttaa cagctctttc tagagcccta
     2521 ggaacccctg taagccattt tctagacaat gttgacatag attatgagga agagtttttg
     2581 gctctctatc gctctcttag tactgaagat gcaattcgaa caattgacta tatgaagcta
     2641 ttaaaaaggc aagaaaatga acgcaatcag ctattgaaag attgaaaaat aaataaccca
     2701 ccgaacttta acggtgggtg tattgagatg aaataagatt aagagctatg gataacctgt
     2761 ctagtttaat ataattgggt ctcttctatt ccttactctc cgtccatcat ctcttgttac
     2821 acttgtaaat tgataaatag gaactaattc aactcctgat aatatcaatt cttttattac
     2881 ttcatctatc cctgcccctt tcgctagata tactaaaaaa ctactgcttg ctttcacatt
     2941 gtatagtttc ttaatatcct tagaagctcg ttcaagagtg aatccttgtt taacaaagta
     3001 gcctctgttt tctattaaag catttttcac gctgttatcc ctgacatttc gtaggcgttc
     3061 acaaacagtg ttaataagtt ctttttctgt cattagatat gtcccttgcc acgataagcc
     3121 ctgtaaaact tcatgtgctt catctaaaat caattgtttt tccttgctaa gttctgatcg
     3181 ggaattttga ggaaatactc ttttagcctc tttttcgtta aaacatatct taacccattc
     3241 ataagaaaaa tattgatatg taaaatgaaa tttttccaga agtggaagga tatcttcatt
     3301 caatttttga atcacagaag ttgcattaaa aacgtctaat cgatagacat tgcttctctt
     3361 atgtttaaag tttcctaacg ctttctcttc ctttaaccta tttaggtatt cgagcatctt
     3421 caaaggaaca tcttcatcat ctaatttttt tatcaatcct ataaaagtca taagtgatag
     3481 taacttatta catttatcta tgtcataagt tttaccatag taagaactca tttttttagc
     3541 taattgttcc cccgacaaaa tacaagtcac actttcctta ctatcatcaa aaaaattaac
     3601 atcagaaagc aagacatcat agacacgtcg catttcctct agataattcc ttattgtcct
     3661 gaaaagtttg tctttcattg attcaagagt gtcaaaaagt acttttgatt cctctttcaa
     3721 ttttcgaaaa tgtgtaggat acaagtctag gttctctaat agccaattaa atgtttctac
     3781 ccgactgtct ttctttaaaa ttagcatcac aactccaata aaattttcac tgatatcaca
     3841 attactggaa tgacaataaa aaatggaatg cccagtttta ctggtaaaaa tactagcaga
     3901 gggattatga tcgttgtgaa acagacatct taagttttta cttgacttaa gtaatttatt
     3961 catatcaatg gttaacaatc tttcataaat ctcattaaaa gacatgtcgg aggaatcaaa
     4021 caggcagtct ttaaaccact gtttcatttc ttctttgtcg tcatttttta tcaacttaac
     4081 aaaatcggtt tcattgacta gttcatactc tctccctgaa tcactttgac aatttttaga
     4141 acatgtcatg gtaaaatcaa tcggaagctc agtaatatct aacatgttgt cttcattaat
     4201 tattatcact gcatctgact tatatcctcc aaaaaacatt ctattcgaag catttacatt
     4261 gtcatcacac ccggggatgt tctttttcaa gtattcgtaa acagcaacca ttttaccata
     4321 gtcagtaatt gcttctttta atagaaagac aatcttgtat ttttctagat tttcagagta
     4381 ccgaatactt ttttgtatcg caaacgcaga attatgaact aaattgatag ataaaacctt
     4441 ttctatgctt aagtatctac tatcatcttt actcaaatct actaccttcc ccttctcatc
     4501 tttaacagtg ttatcgacat ccagaataat tactctggtt tccacaaaac tctccttgtc
     4561 attgccttta ttctttgtct tgcttggaat aaatgattgt gatgaattta gtatctctac
     4621 aaactcatct cttgtaactt ctctatagtt atctaaacac ctatctctta catcagtttt
     4681 ttcttttttt gtaactggct tctctaataa tttttcttta tctacataaa ttggaatatt
     4741 aatcattgat gtttccattt gtgtttcctc cttataaaat tctgttaaag actaaaacat
     4801 acagataata aaaacaaact ctttattact atatgtttta gtcttatttg tttactcaac
     4861 ctttataccg ttccatgaaa ctatcaatat cagatttttt aaatctaatg accgtatcaa
     4921 tttttataac aggtaacccg tattgagaaa catattttct aatcgttcct actgatacac
     4981 ctagatattc acttgcctgc tgatagtcca tatactctag agtgactcca gaaataatgt
     5041 cttctagcat aggctttagc actgtaatta tctctttcac ttgttgttca acttgtatag
     5101 aaacaatgct atcaatgatt tgtagagaca tatttccccc tcctttcgtg attgcttgaa
     5161 caaattttct aagaattatc ttcgtatttt ttgctcaaaa taaaagtatt ttgttattaa
     5221 ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca tacacataat acctcgataa
     5281 aaaaggggtg tcaatagttt tgtaaaattt atttacaaca cccctttttt gaggtatact
     5341 aattttagga ggtgtggtca atatgataaa acgtccacca ataaattatt tagaacgaaa
     5401 aaagatactc ggaacaaaaa taaaagctat aagaaagagt aaaaaattaa cacaaccagc
     5461 atttggtctt atgattaaca acggtcaatt aattgacaaa aagactatct acgaatggga
     5521 gaagggaacc tatttaccaa taccagagcg attatcccga attgctgatt tgggaaatat
     5581 gagtatagag gagttagttt gtggcaatgt agaagaatat attttgggaa ttattcttta
     5641 tagagatagc attgtactag atggtataac atttccagat aaaaacctgt tccagcatct
     5701 tcgtcaacag ttcccaccag tacactctaa tttagataca tggctggata gatattccaa
     5761 attagaacca gagatgcaag aatttattgc taacaagact tgtaataaag ttaaaaatga
     5821 aaaaattagt cttttcaata tcttaaaaat agaagaatta ttcatcaatg ctattgtaga
     5881 agaatttgac aataacattc ttttcctcac tagcagtata gaagaactac tagaaagaat
     5941 ggtagatgaa tggctaccta ttcaattgaa agacatgtct tatcccgaag aagctgtgag
     6001 agaaattact gataatataa acaagctaga acaaacgatt tcatcaattg ggaaaaaata
     6061 cactaagaaa aaaatgaagg gaggtgatac catctagtgg taagtactcc ttgtaaacta
     6121 agaggagatg atttcatggc aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg
     6181 tggatgtata aaggacacat tgccaccgat ccacgaactg gtgaaaaaat aaatactacg
     6241 agaagaggtt ttagtagcaa acaagaagct caaaaagatt atgacgagta cagacatcaa
     6301 atattatacg gtgttaaaaa gaaagcacct gatatgacat ttgaagacct gtataatgaa
     6361 tggataactc atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac
     6421 gcaaataatc aaatacttcc agcatttggt aagcttaaac tatcgaatat ctctgtccca
     6481 tattgtcaaa aagtggtaga tgaatggcac agtaaatatg aaagttacga ttatatgaga
     6541 aaacagactg cacaaatttt acgctacggg gttgcaatgc aatatattga caataatcct
     6601 atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa attttactca
     6661 aaagaggagc ttaataattt actggatgct tttaaagatt ttggtaacat gaaacaatac
     6721 gccttcttca gattattggc atacacaggt atgagaaaga gtgaagtact cgctttacag
     6781 tggaaagaca tagacacgtt taataaagaa ctacatgtca ataaaactct tgctgtagat
     6841 gaatttggta aagttataat tcaatctccg aaaacaagag cttctagacg agttatctct
     6901 ttagatactg agactctttc tatcctcaac aactggaaat tacagcaaaa agaagagtat
     6961 ttaaaacttg gatacaatac tagctctaaa gagcaacatg tttttacaac agttaaaaac
     7021 actctataca ttccaaacac tgttaatgat tggttaagat atattttgaa aaaatataac
     7081 ttgcctcgta ttacacctca tgggtttaga catacacatg ctagtttatt attagaagca
     7141 ggagaatctg tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa agtgacatta
     7201 gacatctatg cacatattac aaataatgca cctaagaaaa caggtcaaga ctttgctgat
     7261 atgatggctc atcagtaaaa gagctggtca aaatctggtc atttctagta tacaggcaaa
     7321 acaaaaaagc tagaaccctt atatatcaag ggttctagct cctataacag gtttacccaa
     7381 cactgccttc caatgactct tttgggaatc agaggaaaga atcaggcttt atagagatat
     7441 ttcatctaac aaattacttt agactgacag tatatatcag caggtactgt catcgggaac
     7501 gtagccaata tcgtatccaa aacagcaggt agagaagcta tttgaggatt caagttattc
     7561 tatttcaacc atgaagttaa gttcattaaa aataaagata gttggtattt caataatttt
     7621 tgtatagagt taatcacttt attgatgcta attcatcttg attggtatta taatcgtatt
     7681 caagttgcgt ggcgtgcttt tgctagaaaa tatataactg ttctttataa agaaatataa
     7741 ggattaaaca ttttttatca tagagtttta cttatctata gaattatgtc tgttcctaat
     7801 tcagtttgat gaattaactg ataaaaaaat atctactatt taaagataca gggataagta
     7861 agtaaaaaaa taacatgtca ttagatagta gacagttcca actctttatt tacctttttt
     7921 gtatgctgca ctgtcaatcc aagttcctaa attgttgtat ccatcatcac ttatataatc
     7981 atattggcca tcaataattt cgtcaaacca caaatattct ccatctggtt tttgcccaat
     8041 tattttaatt actgattgac tagtagttgt catctggtta aaatctttta ttttacctat
     8101 attaacggcc aaaatagcat ttcctcgctt ataactctcc tggatttcat atttaacata
     8161 ttctcgatca agagtttctt tacctatcag caccacagtc actgatgttc caattaattg
     8221 attatctatc cagtttttta tagcaatatc tccattacgt ttaactttct caaattcagc
     8281 cttatctaca aaaccagctt cctttgttcc tcgcaaagtc cagctgtttc taactaccat
     8341 tgctcttgtt acatctgcat aatggaaact aaaaaaaacc cttctcacca tattaatcct
     8401 cctgaatact atttattaaa ctgacaattt gttcaacttt tttatctact tctccttcag
     8461 tatctaattt ttctagcgct tgaaatttac tttttaagga atcatcaaaa taatcactga
     8521 aattctcttg aatcttatca aacaaaacct tacttgcacc tttggtaaat ccaatcggaa
     8581 ctactatatt ttcattttga acagataatt caaattcttc aatcattcca tctgctgtaa
     8641 ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat actaatccca gactctgcaa
     8701 taatgttttc tcgatatctt ctccactcat gaggcatatc aaggggaagt ggtcttaaag
     8761 aaagtacgtc ttgtatgtta ttttttctta aatttcttct ctgctttaga actcctgaaa
     8821 caatactatt accaattcca agtccgtaac ctgatactat tctataaccg gattcagaaa
     8881 gcctatatgc taatttctcg acaaatttta atcctttcga ttcagatgta aatggagtat
     8941 aatcaacagc acttcccgaa ataaaaatag tttttctcaa atactttttt ctaatacttt
     9001 tcaaaatatc agtaatctca gaataatcat ctacttcaac aacgtttatc gaaaatctgg
     9061 acaaatcatt gatttgtagt cttctttttg tcagttcata attatatctc tcttgatcaa
     9121 aaaaatcctc agaatcgaca aaattttcct ttaaaattat atgggatttt agattatctt
     9181 tttagaagtt cttgaatgtc ggtatagccc gtgtttccgg gcttttccgg catttttgat
     9241 attgttagat acttacaaat gttcttatta cctctcacca tttccccctc aaaagtagta
     9301 aatggagtag taaaaccggc cttgtgtcag gctgccagcc gttccatttc tgcccttgcg
     9361 gaatcgaatg tggcgtgtgc ataatagttc agcgtcatgg tgatattgga atgtcccata
     9421 atgtactgca aggctttggg gttcattccc gcattggcta accgggtgca gaatgtatga
     9481 cgcatagcat ggggcgtcgt cgttttcggc aacgcttctt catggctctt gttgtacttc
     9541 ttcacaagcc tgccaaacat cccgccataa tctaccgccg tcatgggata gccgttctgt
     9601 ttgagaaaca ggaaattggc atacccgtca ataatgaacg gctttgcacc tttgcggttt
     9661 tttatcactc gctggaacgc ctgatacact tcctcattca tggggatttt tctgacgccg
     9721 ctttgggtct tgggttcgtc aatatagtaa ccgacgccgg tatttctcaa aagctggtgg
     9781 ctcacgatga ttacccggtt ttcaaaatcc aaatctttgt ccgtcagtcc gcacagttcc
     9841 gaaatgcgaa gccctgtccc cagcagaata acgattgcgt cataatgctt ttggtaaacc
     9901 ttatcacctt gaataaagga cagaaaactt tcttcctgtg ctggtgtaag aggcaccttt
     9961 ggttccgtat cgtcgtccag aacgtcacta agctggaagt caaacgggtt ttttcggatg
    10021 caatcgtcct gtatggcggt gtaaaaagcc gctttcaggg aacgcttgtc gttattgatg
    10081 gtcttataag aaaggccctt ttctttcatc cgaagcgccc attcctttgc gtcggacagc
    10141 ttcacactgt caatcgggca gctccccagc cgatcttgtt ccagaatacg catcagccgt
    10201 tcccggccca gtttggtgtt gtgcttcaca ttgccccggt gcctgatctg cttggcgtag
    10261 agctggcaga cggtcatttt cttgccgatg gtgtctatcc cgtcgtcaag gtctttctgg
    10321 atttctttct ccttttcccg gagtgaaata tcctcccgtt tacctgccgg ggtcttatcc
    10381 gtaggcacta acttccatga gtaaacaaac tgcggttttc caaaggtatc aacatatttg
    10441 taagcatatc gtccgtcttt tctctggctc tctccagacc gcaaaacgcg atttttgctg
    10501 tctcgtcttt tttctgacat ctcgtcatgc tcctttccat gacggaaaga gccgcatata
    10561 tgcttataaa ttattataac atatctgcgg ccctttttca ctattagatt gtgtccaaag
    10621 tgtcaattat tttctcaaac tgcttgcgtt tgatctggat gcggttgcca ttcataatga
    10681 cccagccaga ggccggattt tcctcggcca gcttacgcaa tttgttttct ccgatacgga
    10741 aatatttaga cgcttcctca atcgtcagcg tgtatttttc ccaaataggc acatcatggt
    10801 tcataatgca gccccctttc acaaattcaa aggtttgtgt gatttagggg catagacgga
    10861 cggctgctgg cacagctcca cgggactttc acccccgcgt ggttctcacg cggccctacc
    10921 cattgcctgc gacgctttta acgctcggac tgtggctata agggagtatc atagtcctgc
    10981 ctgtacgtcg tcgcccgcaa gccgccacgc tcacattgcg gcgtggtgtt cctcgctccg
    11041 cttttcccgt agggaaaagt atcatggcgc accagccgtc cggctcggca cagacagaat
    11101 gtgtccgtat gccctatgat gcgccgccgt tatcttgcgg gcatatttat caaggtgcga
    11161 tcaggccacc gggaaaacac cgaaacgggg aaagggtaag ccgtttcatg ctgccggggc
    11221 cggttcgtca gttcgtgacg atagcgtgga tgagcttgat ttccagacgg cgcttcatgg
    11281 cctcgtccag acacacatgc ggatagccgt tttcgtcata gagcgtccgg gtacacagct
    11341 tgttcatgta accgctgtaa tactgcaaaa cgcgctctac ggctccggca tccccagcgc
    11401 gggcggcctc gatcaccgaa aggggcaaaa gctcaatgcc cttgtagttc gggttcttca
    11461 tccgcgtcta acctcccttc ggcatcaacg cctttagctg tttccgcagc tccgataagg
    11521 tatttgtcct gtgccgctga acggcactcc gggacattcc cacaaggccg ccgatctcac
    11581 catctgccat atccagaaca cagtgcagga ttaaaatact ctgctccatg ctgggcagcg
    11641 cggcaaaggc ttcggcaaca agctcattgt tgatatgtag gtcatagcca tgcgacgaaa
    11701 acgtaaagct gtcgctgggg tagtgatcta ctgtacagag cttgtccaaa tctgcctgtg
    11761 acaaagagct tagtgacact tcacggtcac ggcggcgctt catttcagcc aagtagcttt
    11821 tggcctcgtt acgcagtacc gtcttgcaaa aagcatcgaa catgcaccgc gcttcatagt
    11881 cgcgggggat agcatccatc ttctcacccc ctttctgtgg gggacttagg tggttctctc
    11941 cctttccgct aacaagactt tgacaaggcc gccagctacc cgctaaatac ctcctttttg
    12001 aaaatttctc aaaaaatttt tttggggaca gccgggccgc aaaagacgac aaaaatcgcc
    12061 cggcaggtcg caaacctacc gggcgagaaa atgctttgct atgctgctga tttacatggt
    12121 atagtacata ctcataaccg gaatagccct gtg
//
LOCUS       DAYYKC010000083        12135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000083 DAYYKC010000000
VERSION     DAYYKC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.084"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHBBW_002251"
     CDS             <1..610
                     /locus_tag="ACHBBW_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153912.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHBBW_002252"
     CDS             723..1001
                     /locus_tag="ACHBBW_002252"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9153913.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHBBW_002253"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHBBW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9153914.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHBBW_002254"
     CDS             1458..2213
                     /locus_tag="ACHBBW_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9153915.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBBW_002255"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBBW_002255"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9153916.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBBW_002256"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBBW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9153917.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBBW_002257"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBBW_002257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9153918.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBBW_002258"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBBW_002258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9153919.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBBW_002259"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBBW_002259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9153920.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHBBW_002260"
     CDS             6950..7492
                     /locus_tag="ACHBBW_002260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9153921.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBBW_002261"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBBW_002261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9153922.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHBBW_002262"
     CDS             9085..9987
                     /locus_tag="ACHBBW_002262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9153923.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBBW_002263"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBBW_002263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9153924.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHBBW_002264"
     CDS             11430..11852
                     /locus_tag="ACHBBW_002264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9153925.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAYYKC010000084        11898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000084 DAYYKC010000000
VERSION     DAYYKC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.085"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..455
                     /locus_tag="ACHBBW_002265"
     CDS             <1..455
                     /locus_tag="ACHBBW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9153926.1"
                     /translation="ETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRL
                     VKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNK
                     FYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1070..2110)
                     /locus_tag="ACHBBW_002266"
     CDS             complement(1070..2110)
                     /locus_tag="ACHBBW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9153927.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2459..2785)
                     /locus_tag="ACHBBW_002267"
     CDS             complement(2459..2785)
                     /locus_tag="ACHBBW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153928.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2772..3560)
                     /locus_tag="ACHBBW_002268"
     CDS             complement(2772..3560)
                     /locus_tag="ACHBBW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9153929.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3928..4218)
                     /locus_tag="ACHBBW_002269"
     CDS             complement(3928..4218)
                     /locus_tag="ACHBBW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9153930.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4354..4533)
                     /locus_tag="ACHBBW_002270"
     CDS             complement(4354..4533)
                     /locus_tag="ACHBBW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153931.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4530..4880)
                     /locus_tag="ACHBBW_002271"
     CDS             complement(4530..4880)
                     /locus_tag="ACHBBW_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153932.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4873..6198)
                     /locus_tag="ACHBBW_002272"
     CDS             complement(4873..6198)
                     /locus_tag="ACHBBW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9153933.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6648..7262
                     /locus_tag="ACHBBW_002273"
     CDS             6648..7262
                     /locus_tag="ACHBBW_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9153934.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7427..7669)
                     /locus_tag="ACHBBW_002274"
                     /pseudo
     CDS             complement(7427..7669)
                     /locus_tag="ACHBBW_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7873..9192)
                     /locus_tag="ACHBBW_002275"
     CDS             complement(7873..9192)
                     /locus_tag="ACHBBW_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM9153935.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9326..9934)
                     /locus_tag="ACHBBW_002276"
     CDS             complement(9326..9934)
                     /locus_tag="ACHBBW_002276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153936.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10572..10829)
                     /locus_tag="ACHBBW_002277"
     CDS             complement(10572..10829)
                     /locus_tag="ACHBBW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9153937.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10822..11091)
                     /locus_tag="ACHBBW_002278"
     CDS             complement(10822..11091)
                     /locus_tag="ACHBBW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9153938.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11222..11401)
                     /locus_tag="ACHBBW_002279"
     CDS             complement(11222..11401)
                     /locus_tag="ACHBBW_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153939.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11499..11735
                     /locus_tag="ACHBBW_002280"
     CDS             11499..11735
                     /locus_tag="ACHBBW_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153940.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3671 a         2151 c         1822 g         4254 t
ORIGIN      
        1 atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc atcgatgcag
       61 atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca gcctatgctt
      121 ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca gataaagcac
      181 catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa gggacagaac
      241 atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca gtaaagagac
      301 gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc atggaagcca
      361 tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt tcggtctgta
      421 ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta agggatttta
      481 ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt aacactgaag
      541 ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga gattaccagt
      601 ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca atttatcgtc
      661 caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa atttaaaaca
      721 actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat
      781 atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact
      841 caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat
      901 atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac ttttaatcta
      961 aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa
     1021 aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc
     1081 aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct
     1141 ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca
     1201 aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc
     1261 ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta
     1321 attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt
     1381 aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac
     1441 tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag
     1501 tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg
     1561 cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt
     1621 tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa
     1681 ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta
     1741 cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat
     1801 ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag
     1861 atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa
     1921 atatattatc atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa
     1981 gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa
     2041 ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg taataattaa
     2101 aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa
     2161 tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac
     2221 tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa
     2281 gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat
     2341 attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac
     2401 tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt
     2461 aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg
     2521 actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg
     2581 tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta
     2641 cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt
     2701 ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag
     2761 cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta
     2821 aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca
     2881 gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca
     2941 ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc
     3001 ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt
     3061 accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca
     3121 actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt
     3181 ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag tactctttta
     3241 agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg
     3301 gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct
     3361 tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct
     3421 gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc
     3481 aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct
     3541 actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata atataattat
     3601 atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta
     3661 ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat
     3721 taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc
     3781 gagcagcaac tagaataatt taaacattac taaactgtct atattgttac taataattcc
     3841 cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat
     3901 cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt
     3961 aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat
     4021 gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg attttattct
     4081 acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata actcacaaaa
     4141 ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac
     4201 aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg tttgcaataa
     4261 tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa aaaaggattc
     4321 atagagataa ctctatagaa tccgtaaaat accttatata tataacggga agatataaaa
     4381 cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct caacaccaac
     4441 ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat caaaggttat
     4501 atactttagc tcatattgct ggataatgtt cattctaatc gactccattt attatcctgg
     4561 taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc
     4621 agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc ttcagcgttt
     4681 cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc
     4741 gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg
     4801 tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca
     4861 cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag
     4921 tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta caatcgacgt
     4981 aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt cgtcaatttg
     5041 cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata
     5101 ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca
     5161 ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt gtgaaaaccc
     5221 tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg ttttacagtt
     5281 atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca gctcgatttc
     5341 atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt tttcctttgt
     5401 cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga agtacagctg
     5461 taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag caagatctct
     5521 aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa ttccccaaaa
     5581 gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg tccattccgc
     5641 aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca aaaggggatt
     5701 atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct cctggatcat
     5761 ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa gattcagtga
     5821 tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt catcagccac
     5881 atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt tcatacgtgg
     5941 tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc tactcacatt
     6001 tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc ctgaaccagt
     6061 attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat ttctttctat
     6121 acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat ttactggctc
     6181 ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa catatgttcg
     6241 atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat gctatcaccc
     6301 tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat taatttgaaa
     6361 atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt acggactttt
     6421 ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat ttgagaataa
     6481 gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt agtttttgtc
     6541 tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg cttcctcgtg
     6601 tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg agagttgcat
     6661 acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat
     6721 tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact catcgacctg
     6781 ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg gaagctattg
     6841 atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg aagaagaaaa
     6901 atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc aatcctctcc
     6961 tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagaac
     7021 ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca aacggcgtct
     7081 acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt cgccttgttt
     7141 acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt gctaaagact
     7201 acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat
     7261 agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat aattactatc
     7321 tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa ccatttttta
     7381 cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa tcccctactg
     7441 tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt gatttagctt
     7501 cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct agttcttgag
     7561 actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt ggttccgttg
     7621 gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc ctgaataatt
     7681 catagcttta attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac
     7741 tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt
     7801 ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt
     7861 tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa
     7921 ttcattctga tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac
     7981 taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg
     8041 gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag
     8101 catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc
     8161 agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat
     8221 ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag
     8281 ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc
     8341 ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata
     8401 aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata
     8461 atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc
     8521 acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag
     8581 aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa
     8641 gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt
     8701 attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat
     8761 gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc
     8821 ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg
     8881 agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt
     8941 agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga
     9001 taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag
     9061 ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga
     9121 taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg
     9181 taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca
     9241 gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc
     9301 gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta tcattaattt
     9361 tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat tcttctatag
     9421 ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg aaatttttaa
     9481 tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac atgctcccta
     9541 ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa tcaggtttct
     9601 cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa ataaacctat
     9661 ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag gggatacttt
     9721 cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct ctaattatat
     9781 agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa ttactatctt
     9841 ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct acatcccaaa
     9901 cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta aaatcagcta
     9961 atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct atttttttat
    10021 tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag tggggagtac
    10081 ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat ataaaaatta
    10141 cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta aattctatga
    10201 gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa tttaaaatag
    10261 attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc ctttagtaac
    10321 taacatccca cactacaata gtattataac atatctattc aacttcaaat tgttgaaatt
    10381 taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac cttatatccg
    10441 taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata ttagcttttt
    10501 aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac cctttcctta
    10561 cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc attttcaact
    10621 ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga taaatcatgc
    10681 tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc ttttattaac
    10741 ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag ataatcatcc
    10801 caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt catgtgtttt
    10861 aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga cgtaattatt
    10921 agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata atacaacaac
    10981 tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat taacttgttt
    11041 catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca ttcctttcac
    11101 cccttattta tttctataaa acaattgtac attatattgt acaattaagc aaatgattaa
    11161 tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat aaaaagtgac
    11221 gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc aataaatcta
    11281 acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt tgctgcaatt
    11341 ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt gcaattggca
    11401 ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca atcatcttga
    11461 atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat accagtattg
    11521 ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt atttcgccaa
    11581 caaataggac taacaataga ggaatgattt tattcatatg gatctccttc tttctcttag
    11641 gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg ttggagcgat
    11701 tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat ttcacacatt
    11761 ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt gatatggcat
    11821 tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat aggtgtctaa
    11881 aaaatcagtt aaagtcgt
//
LOCUS       DAYYKC010000085        11825 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000085 DAYYKC010000000
VERSION     DAYYKC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.086"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..525)
                     /locus_tag="ACHBBW_002281"
     CDS             complement(148..525)
                     /locus_tag="ACHBBW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153941.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(825..1706)
                     /gene="galU"
                     /locus_tag="ACHBBW_002282"
     CDS             complement(825..1706)
                     /gene="galU"
                     /locus_tag="ACHBBW_002282"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9153942.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1749..2768)
                     /locus_tag="ACHBBW_002283"
     CDS             complement(1749..2768)
                     /locus_tag="ACHBBW_002283"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9153943.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3401..4405)
                     /locus_tag="ACHBBW_002284"
     CDS             complement(3401..4405)
                     /locus_tag="ACHBBW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9153944.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4418..5467)
                     /locus_tag="ACHBBW_002285"
     CDS             complement(4418..5467)
                     /locus_tag="ACHBBW_002285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9153945.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLRLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5484..6305)
                     /locus_tag="ACHBBW_002286"
     CDS             complement(5484..6305)
                     /locus_tag="ACHBBW_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9153946.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6292..7071)
                     /locus_tag="ACHBBW_002287"
     CDS             complement(6292..7071)
                     /locus_tag="ACHBBW_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9153947.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7088..7558)
                     /locus_tag="ACHBBW_002288"
     CDS             complement(7088..7558)
                     /locus_tag="ACHBBW_002288"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9153948.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7570..7989)
                     /locus_tag="ACHBBW_002289"
     CDS             complement(7570..7989)
                     /locus_tag="ACHBBW_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9153949.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8070..10847)
                     /locus_tag="ACHBBW_002290"
     CDS             complement(8070..10847)
                     /locus_tag="ACHBBW_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9153950.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11038..11262
                     /locus_tag="ACHBBW_002291"
     CDS             11038..11262
                     /locus_tag="ACHBBW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153951.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3429 a         2486 c         1913 g         3997 t
ORIGIN      
        1 gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa
       61 aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta
      121 tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc
      181 gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt
      241 tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac
      301 gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc
      361 tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa
      421 tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt
      481 gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct
      541 ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc
      601 atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat
      661 aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac
      721 tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt
      781 aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt
      841 agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga
      901 agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt
      961 cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt
     1021 agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga
     1081 gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt
     1141 tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt
     1201 ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt
     1261 tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc
     1321 cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc
     1381 cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca
     1441 actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt
     1501 gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg
     1561 attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga
     1621 agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca
     1681 ggaatgactg cttttttgac cttcacttgc tgttcctcct aaacgacgat taactattga
     1741 tcatctattt aacgaagaga aaattcattc tccatttttc cgtcacgtaa catgatttct
     1801 tttgctgctt ttttgatatc ttctccatta tacaaaacat tatagattgt ttgagtaatt
     1861 ggcatctcaa cacctagctg ttgagctagc tctactgctg cttttgtcgt tgccacaccc
     1921 tcaacgacca ttcccatgtt ttctagaact tcttccagct tatgtccctg tcctaacaag
     1981 ttaccggcac gccaattgcg agaatgaaca cttgtacaag taacgatcaa gtcccccaca
     2041 ccactcaaac cgataaatgt cagaggattg gcacccatcg cgacaccaag acggctgatt
     2101 tctgccaatc ctcgagtcat gattgctgct tttgcatcat ctccgaatcc taatccatgg
     2161 atagcgccgg caccaattgc aatgatattt ttcagtgctg caccagtttc tacgccaatc
     2221 acatcattat ttgtataaat cctgaaataa tcattcatga acagtttttg tacataggct
     2281 gctgcttttt gatcaatact tgcagctgta attgttgtga tatcatgtac tgccacttcc
     2341 tcagcatggc taggaccaga taatacaaca attgcctgtc ttttttcttc tggaatttct
     2401 tcagccaaaa cttctgagat tcgtttatgc gtatcttgtt ctagtccttt acttgcatgt
     2461 ataatgattg gcttagtatt cattttacca ataagttcct gtgcgactga acgaatcgct
     2521 tttgttggaa caacaaataa aatagcatct gcgttatcca ctgcttctgc caattcttta
     2581 tatccgataa tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt tgtatgatac
     2641 ttattgattt catcaatctg ttcaggtaca tttccccaaa tacggacatc atgcccgttt
     2701 tctgccaatg tttgtgctaa agcagtgccc caagatccag gaccaagaac tgcgattttt
     2761 tgtttcatgg atattcctcc aaataaataa ttgataattt ccattatagt atacatttct
     2821 taaagttggg actaatttcc attgttttta agtgtttgtt tacttctttt atcgttttta
     2881 cttgaaataa tcaatgatat gttacaatag tctacatttt atttttacac tcgttatgct
     2941 ttgcttgttc ctaagaggaa gcagttttga caatgttact accagcatta ctattaggag
     3001 gttaggaaac ttctcctagt gagtattgat ttaaaataca taagagacag attgaacatt
     3061 tctaaatctg tcaggtcctg caacggacag attgtctctg ttgacggttg acataggaac
     3121 ttttgtttta gtcaacattc tggatacaaa acgctatgta aactggcgca actcgttgta
     3181 cttggacttt tactacggat ccatgcaaca actaaatagt actattgcat aacaaaaaat
     3241 ggttggcacc cacatgtccc attaaacaga ttataagttg aactgtagat aatgcaaaaa
     3301 atgtgtttta taacgtaatt gatgccaata ttttaaaaaa ataaagggag cagttatcgc
     3361 tgctctcttt attttttata ttctttttat ccagtctatc ctaataatca aactgacgat
     3421 agtagcggcg tatctctaat ggatggcagt tcaactgctc cacatgagca tcgatgcgtt
     3481 gtgtcactac atgcatcaat aagaaagaca aaacacgtcc gcgatatttt tcactgatac
     3541 cttctactgg atagtctttt gtgtctatca atgtataatt gctgcaaatt tttggcaaaa
     3601 gtttagccac acgttcagat aacactctct gttcatcttc gcctaaaaat aatgtgactg
     3661 gggttgtttc ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca tggatagatt
     3721 tcgatggaag ccagctttgt tcttcccagt aacacatggc atatgaataa acagctcccc
     3781 attgattccc tgctccaata aaataatgca tactatcacg tcgatgtttt tctgcaaatg
     3841 ataagccaaa tgcatctgct tttttctctg cttccactaa ccctgttggt aaataacgtt
     3901 cgagctgttc atagtattct gaataagctt cgaattctcc attcaaatac atcaatcgat
     3961 ctgctgccat aaaaaatttg atttgttctg ttccaggagc tggataagtg accacaaaat
     4021 cgcatagctg agacaactta ctgttcgctt tatcgataaa tccaatcagt gtcgctccta
     4081 ctttcttgat ttgttctact gctttcacta cttcttgagt tgtaccggta acagaggaca
     4141 aaataacgat agaatctttt gttaatcttt tgtttccagt agtgtaatat tctgcagcat
     4201 gctgtacaaa gacaggcaag ttgcttttcc cattcatata agtgacagct tgcatcgagg
     4261 aagcatacgt tccaccgatt cctagataat agacagcgtc aaatttttcc gcataaagtt
     4321 tatcgatgat ttcttctact tgcggacgta aagctagtgc gccttttatt gctttgattt
     4381 gttcttcttc attgaatttc agcatgattt ccacctctta taatttactt ttcaagactg
     4441 tatcaaagtc ttcaaaaatc tttttgttca agtcaatacc tttagctact gcggttttat
     4501 aagcgaatag ttgaaacgga acgacaagcg ctaatacaga cagatattca tcacattcta
     4561 ttgctaatcc aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg agagtctctc
     4621 caacgtaccg actcatatat ctttgcaact tttgcgaacg ttccttattt acactttccc
     4681 cttcaataaa gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg tgcatataag
     4741 cttctaactc aaatccttgt gacggaacgc ggattgtttc agtcaatttc gtctctgctt
     4801 ctttagctgt tccccagttt ggaccatatc caatggtgac gaaacgctgt gctaaacgaa
     4861 ataaactttg atatccatca aaaaattctt ccgttttttg aataatcgct ggtaaatggt
     4921 tgattatttt gcgcaactgt tgcatgtagt cttgttctat ttttttgctg attttatttt
     4981 tacttattcc aattcctatc cctaagcgta gtaactgtaa aacagtcgca gaatagcctt
     5041 ttgtaacgaa gcctactgat tctaccccgg cacctaaatc aataatttgt gcagcttcta
     5101 aagcaagtgg actttgcacg tcatttgtga tagcaacaac aggtaaccct tgtttttgta
     5161 tcatttttac tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca gcaatcacag
     5221 tatcgattga aggagaaagt tttccataat tgaaaaaata aaaaggttct tgaatatcga
     5281 cagtaagatc accataagat tccaatgctg gcttcgctgc taaacaagcg ttataagatg
     5341 aacccgttgc taagatcagt aaattcactg tttttttcat atgttctctt gtcgaaaaat
     5401 cattcttttt caagatcgta cttagtactt ccttttcttc atagatataa tcttgcatcg
     5461 taaacatttt ctctgctcct tttttaaaaa atccctatcc acgaaccaaa aattccaaag
     5521 aaagctagtc cgatcaagat cgttaatggt ttcacttctt tcttcaatag ataaaacact
     5581 atgccaaatg ctccaagaca taagatattt ggaacgatat catcaaaaat actttgaaca
     5641 gtcacagcat tttctcctga accgattttt aaaggaatat tgatggtgat catcgtggca
     5701 gtcattgctc caacgaccat cagtccaata atagacgcgc ctaatgtcaa actttccatc
     5761 ataccgtttg cctggatctt tttcaaaaaa cccgttccta agcgatatcc ccaacctgtt
     5821 gctagatagc gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa gatagggcct
     5881 aaaatatttc cttgcatggc taaagaggta cccacacctg tggcaatcaa tcgcaaagtt
     5941 ccccaaaaga aagaatctcc taaccctgca agaggtccca tcaaagatgt tttgatacta
     6001 tcgatcgcag tcacatcaaa ttcaggatca tccttatttt cttcctccat tgcagccgtg
     6061 attcctaaaa tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag atgccgtttc
     6121 aaagctgctg ccatttcttc tttcgactga tataactttt tcaaaatcgg aatcattgca
     6181 taaacataag ccatattcat ctgacgttca taattccaag aaaactccat ttgaaaagaa
     6241 cgccaaaata cgtgatttag ctcttttttc gttattttgc tgtttttgat ctcagaattc
     6301 ttcatcttct tctacctccc ctgcagttgt ttgaagtggt agttgacgga cattcataat
     6361 attgactacc acaactgcta aaatcgcacc aaaaatagct actccggtca caggaatctc
     6421 taagtaagaa gctatagcaa aacctaagaa aaaatatggt gcaacttgtt tgttgatcag
     6481 caaacgagca agcatcgcaa aaccaagagc tggaatcaac cctgtagcta ctgacaaccc
     6541 cttttgaacg aagtcaggaa tcatatttaa tagatttccg atcgtattgc ttcccaccat
     6601 ataagaaacc attaccacaa caccaagcat caaggaaaga ccgataccgg caagcaagtg
     6661 catatgctcg actcctttat aatttccttc ttctgcataa ttgtctgctt tatggttcat
     6721 aattggaatc aaaagcccta agtaaatatt tttcaatatc aatgtcaacg tagcaatcgg
     6781 caaacctagt aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa aagccgtccc
     6841 taaaatccca ccggtcacaa catcaggtgg aatcgaagca cctactgaga acgatccgat
     6901 aaatgctaac tctaaagttg cgcccataat gatcccagtt tttacatcac ctaacacgat
     6961 tcctgtaaac agccccgtca caatcggacg agaaagcaac gatgtcccta gtgcatattc
     7021 tgattgtgcg acaaatgcaa caattcctaa taagactgct tgtacaatca ttgaggttct
     7081 tcctttctta caaaacttct cttacatcca cttttttatc tgctggtact tgacggattt
     7141 ctacttctat ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct ggtaaaatat
     7201 ttaccgcttt tccgatgctg cgagtaccag gttttgcctt gatccctcct aagttgatgg
     7261 ttttgatttc tggatacgct tctgccagct tatatgcatc ctcgatcgat tccactacga
     7321 tgaataactt atatttatct gtcaccccgc ttttcaacgc ggcaatcgag tcatcaattg
     7381 atttgatcac tagtttgact ccttgtggct tggctaattt gatcgttgtc tttcgtatct
     7441 cattcgtagg tacatcatca ttagcaatca aaatacagtc tgcgcctatt gtttgtgtcc
     7501 atgaaaaagc tacttgtccg tgtaataacc gatggtcgac tcttgtcaat acaatcattt
     7561 ttcttcctcc taaaattcgt cttcttctat tttctgattg agccgctcgt tacaaaactg
     7621 gatcattttt tttgaatcag ttagcacctg ctggatcaaa acagttgtct ctacttcagc
     7681 gtccaaccga gatactagct caatcaatag tggcaaattc aatccggcaa cgagatagac
     7741 atgatcccga ttgatatatt gtaagaattc gttgttgaca cttccgccaa aaagatccgt
     7801 cagaacaatg atttctttat ctttgtacct cataaaaagc tgccttactg tttccgctaa
     7861 atcaaaattc tcatcagtat agcaatcgaa tacagtaata gaatgatttt tcccatagat
     7921 cagctctaat gaatctaata tccctgaagc aaattttcca tgggaagcta agataatttc
     7981 tctatccact ttttcatctc ctcttttcac ttacacatac taaaaaagca agttgcatgc
     8041 caaaattagt ggcaacaact tgcttatgtt caaaattctt cttctatcga gagaaactct
     8101 gtatcttcac tcaaaatatc atagatatag gcaatctcag aattggggat catgacacta
     8161 taatgctctt caatgacact aaacgcagat ttaatagcag ataacccttc tttttgacac
     8221 tgcttccatt tctctaatcc ttgataggtt tcgattggca tttgtcggat caatcgttca
     8281 atgagacagc tcacatggac atacaacgct aattttttgg gattgctgat gcttcgattc
     8341 aactgcacct ctaattctct cataaaatga tcgatttctc ccatcacctt ctctgtatct
     8401 agaatagtca cggaatccag tactttttcc aatgaaaaat tacgaatgat gcgctgattg
     8461 aatatttcct tctctgaagc attcatagta gtgtccaacc attcagctag tacagacatc
     8521 ttttcccccg agatgaggtc ttctaatgaa agatagtcga tattggtgat atcaggatct
     8581 gtggtgccaa tcattcctac gacttcgtat atggaaaata atgggtcact gtttttctta
     8641 tcttttaatt gctgatattc atatgggatg attttcaact gacaagccgt aggtaagctt
     8701 ttttctagca gttcacttaa gtggaccgcc gtaccgattc cagtgaagca agtagtaatg
     8761 agtgctttgg gtttgttttc ctctggatag agcagtcgcc attctggctg gatctgtcga
     8821 acagtctctt ctgctatctc ttctaatgat acattttttt gcaggcattc ccctactgtg
     8881 attgctaaag gtgtcgtcac attattcaaa atcaaaagcg gtacggataa ctgtttttta
     8941 aaaaactgat ggatttcttt cagagatccc atgtctacta atatgatcaa tccatttgaa
     9001 acatcgctgt tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg tgtgacatct
     9061 agtggcatat caaatgattc aaatagatcc tcttgtaata aacgatttac tacatttgcc
     9121 atactgctag cagttgcata accgtgagca gcaatcatcg cttttggcag tcgtttttct
     9181 tttgaccatt tcgctttttt tagatagatc gtcagtaaaa tccgatccat acttgtgtct
     9241 tcaatatcta atttgatctt acttaattcc agaattcgtt ggacatagtg ataactagat
     9301 ggataagact gagaaacttg ttgttctaat tctttgatta gttggataat atcatgttct
     9361 tccggtaacc atttgatagt gcttcgctga tagagatagt aactcaaggc atagacactg
     9421 tttccatcga atttgatttg ataggcagtt tccatttgtc gaaaggtctc tcggatagat
     9481 tgtgtccaat aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa cagcaaaaaa
     9541 tcaaataatt gaatcacttc ttgctttaat tttccttcgc aagaatatag cagacagtta
     9601 tttttcttga aatcaattag gatttgctca aaacttttaa cgattcgctg ctgttcgggc
     9661 tgttgcttct ccattaattg ctctaaggtc tggtcttgcg taatatagat tccttgctgt
     9721 gctgtatttt tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg gatcgtgaca
     9781 tggatttcag gctgttcacg ctgttcagca tatgcttttg ctgctgaaac tttgacgata
     9841 tttttcagct ctcctatgtt tccggataat ttctgacttg ttaaaagtga caaagctggt
     9901 ccactgactg acaaagggct ccccattttt ctttgttctt cgagaaaaaa agagtaaatc
     9961 agttctagcc gttcattacg actacgctga ttaagtgcag gtagttcgat tcggataggt
    10021 attcttcgaa tgaaagttgt taagaaatga ctagtaatct cttcagtcgt agcaaaaaag
    10081 agacgtgttt tgatccggcg tgacaaatta ggttctccca tacgataaat cacaccttga
    10141 tcaagatagg tgaacagttt ttcctgacct tctggtgtta agcgatgtac ctcgtctaaa
    10201 aacaagatcc caccatctgc tgcttcaaaa gccccttttt tattttcttc tgcaccagtg
    10261 tacgctccct taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt atctgcatat
    10321 tgtgcacaat taaaagtgat gaatggtgca gaatcttcta gcagatcatg aagcagacaa
    10381 tactgataga ccaaattaac taaataactt ttacctgtgc cactttcgcc tgtaatcaaa
    10441 agtggtaatc cgccatctgg atagtaaaga gctgttttga tttgttcgat actttctttc
    10501 aaactggcgt cgtatccgat cataaaagag aataagtctt ttggttgcgc gaaaaaaggc
    10561 tgttcctctt tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt aaaaaattgt
    10621 ttttcaaaag cttctttgtg gaaataaatc actggtcgag aattgatttt gactagtagt
    10681 tgctcatcat ttaactggtt caaataatga ctgactgtgt ttctttttac cccaaaatga
    10741 tcagctaacc tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa aaaggctgtc
    10801 tggttctcta agtaacataa aatttccttc ttcaacaact tattcacccc tcctccatca
    10861 tagaaaacgg attcattttg tcaataaaat ctttaataaa gaattgaaaa atatatctta
    10921 ttatagacag cagcctactt atcccttata atttttattc taaaaaacgt acagttcctt
    10981 ccagttttta tactggaatt tcaacatatg aaagaataag gaggctatga ctattcaatg
    11041 aaatcggata aatataaaaa caggaaacat acttcctgga aaacttttcg agaaatcatc
    11101 ggatcttatc tcgctgtagt tgtatttttg cctttgcttt atatggctat gacagaacaa
    11161 actcgtttta ttggcgtaga tacgcaatat atagccgaaa caggattttt attcatcttt
    11221 atcttgattt ttgcgttcta taaatccttc catacaaaat aagattcaca gatttcaact
    11281 ttcctgtctg tttttagcta ttacaaatcc ttatatgtgg tataacaaca atttattggc
    11341 ataatgagga aaaacgagtt atgatgcttt cagagagtaa agaggtactg ttgtcaccat
    11401 cctttttatt gattacgtac accactctta gttttttccc attgattacg aaaacaaggt
    11461 agaaacattg atgttcctac cttgttttct ttttttattt tcagatcaaa atctcaagtg
    11521 gcttttgact tgatgtcggg aggttgtctt cgttccatca aacctgatag aatctgcgcc
    11581 aaaccatagt tttctccact attatttatt cctctaagat agtacccaca ataaacgaat
    11641 aaatattttt ctcattttca tactccttct ttgaaaatca attagatttc tctcaaccca
    11701 cttttttaca aaaattacgt agcaaaaaag attgcaagaa caaattaaaa aagaagtaaa
    11761 tagagtttca tctataaaca tttacttgat atatttttta tgcagaattc atttatagaa
    11821 aaatt
//
LOCUS       DAYYKC010000086        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000086 DAYYKC010000000
VERSION     DAYYKC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.087"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..776)
                     /locus_tag="ACHBBW_002292"
     CDS             complement(162..776)
                     /locus_tag="ACHBBW_002292"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9153952.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(923..1606)
                     /locus_tag="ACHBBW_002293"
     CDS             complement(923..1606)
                     /locus_tag="ACHBBW_002293"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9153953.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1715..2173)
                     /locus_tag="ACHBBW_002294"
     CDS             complement(1715..2173)
                     /locus_tag="ACHBBW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9153954.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2257..4020)
                     /locus_tag="ACHBBW_002295"
     CDS             complement(2257..4020)
                     /locus_tag="ACHBBW_002295"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9153955.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4241..5701)
                     /locus_tag="ACHBBW_002296"
     CDS             complement(4241..5701)
                     /locus_tag="ACHBBW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153956.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5909..6835)
                     /gene="glsA"
                     /locus_tag="ACHBBW_002297"
     CDS             complement(5909..6835)
                     /gene="glsA"
                     /locus_tag="ACHBBW_002297"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9153957.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6858..8285)
                     /locus_tag="ACHBBW_002298"
     CDS             complement(6858..8285)
                     /locus_tag="ACHBBW_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9153958.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8316..9530)
                     /locus_tag="ACHBBW_002299"
     CDS             complement(8316..9530)
                     /locus_tag="ACHBBW_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9153959.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9616..9843)
                     /locus_tag="ACHBBW_002300"
     CDS             complement(9616..9843)
                     /locus_tag="ACHBBW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153960.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9871..10113)
                     /locus_tag="ACHBBW_002301"
     CDS             complement(9871..10113)
                     /locus_tag="ACHBBW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9153961.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10145..10702)
                     /locus_tag="ACHBBW_002302"
     CDS             complement(10145..10702)
                     /locus_tag="ACHBBW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9153962.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10715..10903)
                     /locus_tag="ACHBBW_002303"
     CDS             complement(10715..10903)
                     /locus_tag="ACHBBW_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9153963.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10916..11482)
                     /gene="amaP"
                     /locus_tag="ACHBBW_002304"
     CDS             complement(10916..11482)
                     /gene="amaP"
                     /locus_tag="ACHBBW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9153964.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3448 a         2437 c         1927 g         3943 t
ORIGIN      
        1 ccatctaaag taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac
       61 tgcccttgca gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc
      121 gatggtttat agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc
      181 gtgaaaattt ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat
      241 tttcttggat cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc
      301 tgtacttcta gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt
      361 tcatataagg atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt
      421 actgaatcag ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg
      481 gctgctgttt tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct
      541 cttgtctcaa gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc
      601 aaggataaga tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc
      661 actatatctt ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca
      721 atcagcaact tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt
      781 tcgccttctt tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat
      841 gcttttttca tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa
      901 gaatctgtgc cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt
      961 tagtgatcaa ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt
     1021 catatgcttt ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat
     1081 cgacttgttc tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat
     1141 attctgtata gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa
     1201 gcgcttcctc aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt
     1261 ggacaagttc agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa
     1321 tgatttctgc ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca
     1381 cttctgaccc atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct
     1441 tgatttcatt gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg
     1501 cagccaaagc cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt
     1561 gacaagaaac gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag
     1621 taatgttata taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca
     1681 ataatgagct atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt
     1741 cgtatacgac cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt
     1801 taaaaaactc agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt
     1861 tccaagcatg ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga
     1921 tgatacaaac tggttcattt tttccattca ggaccacgtc taattgacca acttttggta
     1981 aaggctcatt ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt
     2041 ttacaactag ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac
     2101 ctaatttttc tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta
     2161 cttgctgata cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact
     2221 agtataaaca attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag
     2281 actatcttca acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg
     2341 aacgctatcc gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc
     2401 ataataacga ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat
     2461 gactaattcg tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt
     2521 tcctggattg atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt
     2581 tcctaattca agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt
     2641 ttgataggct ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt
     2701 aggaattcgg attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat
     2761 catatcctct tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg
     2821 aatgtcaaat gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa
     2881 aataatcact ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg
     2941 caagaaagta cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc
     3001 gaaagtgata gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc
     3061 ttggaatgtt ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc
     3121 tgtacttgtt tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc
     3181 agaagttact tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt
     3241 cattttatta tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac
     3301 aatcggatga tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt
     3361 tccattatcc acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt
     3421 accgttggtt tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat
     3481 agacatctca ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt
     3541 caatccttca aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt
     3601 tcctgattct ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt
     3661 agatgtgtgc cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct
     3721 cacttcacgg taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc
     3781 ataagactga tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg
     3841 aaacgcaatc gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct
     3901 gtccttttgc aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga
     3961 taattttttg acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat
     4021 aatacattct ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc
     4081 tattctacta cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag
     4141 cttgttatat aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca
     4201 attaaaaagc tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta
     4261 ataggtgaag ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg
     4321 gatagatctt tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa
     4381 ccactctttt tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa
     4441 taggatttat ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa
     4501 attgtttgac tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt
     4561 cactgttgat caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta
     4621 gttggatttg atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa
     4681 gttgttcttt ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc
     4741 acaacgtacg gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga
     4801 tttcagcgat actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa
     4861 aatttttata aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat
     4921 ttctactgtt gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc
     4981 ctttttgctc ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt
     5041 tcttatcttc ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc
     5101 ctcgtatgat aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta
     5161 cataatcaat caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt
     5221 cccaattgac ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa
     5281 tgatttcttt atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga
     5341 ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata
     5401 taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg
     5461 tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta
     5521 cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg
     5581 ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa
     5641 ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca
     5701 ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta
     5761 tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg
     5821 acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga
     5881 cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga
     5941 ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc
     6001 tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc
     6061 tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag
     6121 ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc
     6181 aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga
     6241 cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca
     6301 tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag
     6361 aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg
     6421 acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa
     6481 tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca
     6541 tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta
     6601 ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa
     6661 agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca
     6721 tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg
     6781 tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa
     6841 taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc
     6901 gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca
     6961 tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa
     7021 gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag
     7081 gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag
     7141 tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc
     7201 cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc
     7261 atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct
     7321 tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg
     7381 atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca
     7441 atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt
     7501 tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata
     7561 accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga
     7621 ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta
     7681 ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg
     7741 cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca
     7801 gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt
     7861 aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt gttttgtata
     7921 gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag
     7981 ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat
     8041 gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct
     8101 gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact
     8161 aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg
     8221 gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt
     8281 tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag
     8341 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     8401 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     8461 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     8521 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     8581 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     8641 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     8701 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     8761 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     8821 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     8881 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     8941 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     9001 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     9061 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     9121 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     9181 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     9241 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     9301 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     9361 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     9421 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     9481 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     9541 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     9601 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     9661 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
     9721 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa agaatacttc
     9781 atataaaata tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt
     9841 catttcaaca taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac
     9901 taatactaaa atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg
     9961 tccccaagtt cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt
    10021 tgcgatgatc cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat
    10081 accgccgacg attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta
    10141 cttcttattg tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag
    10201 cagattttgc tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg
    10261 ttactttgtc ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg
    10321 caacattcac attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga
    10381 tcaattcttt gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat
    10441 ctagatccac agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat
    10501 ctgtgttgac taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac
    10561 catctacatc tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca
    10621 attcaccatt catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg
    10681 tattcatgtt tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta
    10741 ttcctgactg tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca
    10801 acaatgtctt gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc
    10861 caaagataat agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt
    10921 ccactcgaga gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga
    10981 ctgccacttt gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt
    11041 gcatcaatac acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat
    11101 ggatatggat tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt
    11161 cttcgtgtaa atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc
    11221 catcttcccg tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca
    11281 agatgataat agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt
    11341 agtaattcgt taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg
    11401 cattgatcaa cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga
    11461 ttatttttaa ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata
    11521 actctaccga caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc
    11581 gctaccaaac attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca
    11641 attgcttact tattctctat ttacttttga ttgctggcta tacttaaagg atattattca
    11701 agattaaaaa ttgcaaagat tatgctcagt tattattaca agattctatt tattt
//
LOCUS       DAYYKC010000087        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000087 DAYYKC010000000
VERSION     DAYYKC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.088"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHBBW_002305"
     CDS             <1..177
                     /locus_tag="ACHBBW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9153965.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHBBW_002306"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHBBW_002306"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9153966.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHBBW_002307"
     CDS             1199..1771
                     /locus_tag="ACHBBW_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9153967.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHBBW_002308"
     CDS             complement(2023..2280)
                     /locus_tag="ACHBBW_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153968.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHBBW_002309"
     CDS             complement(2406..3221)
                     /locus_tag="ACHBBW_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9153969.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHBBW_002310"
     CDS             3738..5063
                     /locus_tag="ACHBBW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9153970.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHBBW_002311"
     CDS             5056..5406
                     /locus_tag="ACHBBW_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153971.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACHBBW_002312"
     CDS             5388..5573
                     /locus_tag="ACHBBW_002312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153972.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBBW_002313"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBBW_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9153973.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHBBW_002314"
     CDS             complement(6724..7677)
                     /locus_tag="ACHBBW_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9153974.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHBBW_002315"
     CDS             8289..9782
                     /locus_tag="ACHBBW_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9153975.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHBBW_002316"
     CDS             9917..10213
                     /locus_tag="ACHBBW_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9153976.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHBBW_002317"
     CDS             10314..11165
                     /locus_tag="ACHBBW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153977.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHBBW_002318"
     CDS             11186..11398
                     /locus_tag="ACHBBW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153978.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1810 c         2179 g         3725 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagtt
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAYYKC010000088        11669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000088 DAYYKC010000000
VERSION     DAYYKC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.089"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBBW_002319"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBBW_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9153979.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHBBW_002320"
     CDS             complement(324..893)
                     /locus_tag="ACHBBW_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9153980.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHBBW_002321"
     CDS             1079..1981
                     /locus_tag="ACHBBW_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9153981.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBBW_002322"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBBW_002322"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9153982.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBBW_002323"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBBW_002323"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9153983.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBBW_002324"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBBW_002324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9153984.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHBBW_002325"
     CDS             8549..8734
                     /locus_tag="ACHBBW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9153985.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHBBW_002326"
     CDS             complement(8796..9992)
                     /locus_tag="ACHBBW_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9153986.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHBBW_002327"
     CDS             complement(10162..11598)
                     /locus_tag="ACHBBW_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9153987.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3449 a         2485 c         2048 g         3687 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaa
//
LOCUS       DAYYKC010000089        11661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000089 DAYYKC010000000
VERSION     DAYYKC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.090"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..532
                     /locus_tag="ACHBBW_002328"
     CDS             326..532
                     /locus_tag="ACHBBW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9153988.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(734..1735)
                     /locus_tag="ACHBBW_002329"
     CDS             complement(734..1735)
                     /locus_tag="ACHBBW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9153989.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1740..3653)
                     /locus_tag="ACHBBW_002330"
     CDS             complement(1740..3653)
                     /locus_tag="ACHBBW_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9153990.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3821..4327)
                     /locus_tag="ACHBBW_002331"
     CDS             complement(3821..4327)
                     /locus_tag="ACHBBW_002331"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9153991.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4487..4927
                     /locus_tag="ACHBBW_002332"
     CDS             4487..4927
                     /locus_tag="ACHBBW_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9153992.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4953..5981)
                     /locus_tag="ACHBBW_002333"
     CDS             complement(4953..5981)
                     /locus_tag="ACHBBW_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153993.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6036..6110)
                     /locus_tag="ACHBBW_002334"
                     /pseudo
     CDS             complement(6036..6110)
                     /locus_tag="ACHBBW_002334"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6113..6469)
                     /locus_tag="ACHBBW_002335"
     CDS             complement(6113..6469)
                     /locus_tag="ACHBBW_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9153994.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6767..7741)
                     /gene="sgrA"
                     /locus_tag="ACHBBW_002336"
     CDS             complement(6767..7741)
                     /gene="sgrA"
                     /locus_tag="ACHBBW_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9153995.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7937..8776)
                     /locus_tag="ACHBBW_002337"
     CDS             complement(7937..8776)
                     /locus_tag="ACHBBW_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9153996.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8961..9848
                     /locus_tag="ACHBBW_002338"
     CDS             8961..9848
                     /locus_tag="ACHBBW_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9153997.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10442..11136)
                     /locus_tag="ACHBBW_002339"
                     /pseudo
     CDS             complement(10442..11136)
                     /locus_tag="ACHBBW_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11120..11518)
                     /locus_tag="ACHBBW_002340"
     CDS             complement(11120..11518)
                     /locus_tag="ACHBBW_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9153998.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3479 a         2313 c         1747 g         4122 t
ORIGIN      
        1 taggttatca tttgtaattt tatttcacag ttgatatgat tgttatgtaa aataaaaaag
       61 tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat cttttctatt
      121 atattcccat attttctatt attggtctag acgtatgatg gtaaactttg tacctatctg
      181 attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc
      241 attttattca catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac
      301 aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc
      361 tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg
      421 aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc
      481 cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa
      541 atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg
      601 tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag
      661 cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt
      721 gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt
      781 gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat
      841 ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt
      901 tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat
      961 gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt
     1021 aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg
     1081 cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag
     1141 tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc
     1201 cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat
     1261 cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat
     1321 gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac
     1381 atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat
     1441 gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat
     1501 tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt
     1561 tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt
     1621 ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta
     1681 aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt
     1741 taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct
     1801 gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca
     1861 tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga
     1921 tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt
     1981 ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa
     2041 atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct
     2101 gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga
     2161 cttttattgt tttcatccac atactttacg tttatctgat catggaacac tactttcaaa
     2221 aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct
     2281 gctccttcta ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg
     2341 ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct
     2401 gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag
     2461 tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac
     2521 tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt
     2581 tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata
     2641 tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc
     2701 ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca
     2761 tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat
     2821 aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc
     2881 caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa
     2941 tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt
     3001 tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca
     3061 agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca
     3121 atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact
     3181 aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc
     3241 tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca
     3301 tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac
     3361 atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga
     3421 tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta
     3481 ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc
     3541 gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca
     3601 aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt
     3661 tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt
     3721 tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata
     3781 aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca
     3841 gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag
     3901 gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag
     3961 caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat
     4021 gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg
     4081 tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt
     4141 ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg
     4201 tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg
     4261 gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt
     4321 ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag
     4381 ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat
     4441 ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga tgattaaatt
     4501 gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga
     4561 tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca
     4621 cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta
     4681 taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg
     4741 tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac
     4801 tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct
     4861 caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat
     4921 gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa tttttccaat
     4981 atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg cgcccaatca
     5041 ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc atatgccttt
     5101 ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga tgcactatat
     5161 tttattgggc gttcagtcat agataatatc cttttctttg cccataattc ctgtgtttta
     5221 ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa aatatccctg
     5281 actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt attaagctct
     5341 ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat cactggaaat
     5401 atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt cttttttgtc
     5461 ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata atttttttct
     5521 gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga cgattcaatc
     5581 cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc caaataggtg
     5641 ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat gtgattcatc
     5701 gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt
     5761 atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga gtggccttgt
     5821 ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc tatactacgc
     5881 gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt tatacttgat
     5941 aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg
     6001 atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga taaatgatag
     6061 tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa acttatataa
     6121 ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa
     6181 tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa aactgagaag
     6241 aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc
     6301 tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta
     6361 gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa caatagttca
     6421 aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc
     6481 ctttttatct attatttaga cttattccga cagctacatg aataacgtac aaatagattc
     6541 gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct
     6601 tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata
     6661 gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc
     6721 aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat
     6781 ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg
     6841 taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg
     6901 tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca
     6961 cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca
     7021 aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac
     7081 tctcactact catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7141 tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg
     7201 tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt
     7261 ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac
     7321 tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa
     7381 ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact
     7441 caataccagc ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac
     7501 ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt
     7561 tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag
     7621 ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa
     7681 tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca
     7741 tttctatcac tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa
     7801 tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga
     7861 atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc
     7921 ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat
     7981 atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg
     8041 cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga
     8101 aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat
     8161 tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc
     8221 tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca
     8281 atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc
     8341 taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa
     8401 taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat
     8461 acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga
     8521 gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc
     8581 tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga
     8641 ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat
     8701 tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat ccaatcaacc
     8761 gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc
     8821 ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact
     8881 aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga
     8941 ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc
     9001 tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa
     9061 cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa
     9121 ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa
     9181 aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc
     9241 caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct
     9301 ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag
     9361 atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt
     9421 cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca
     9481 agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt
     9541 ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa
     9601 acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt
     9661 atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg
     9721 agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa
     9781 tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat
     9841 tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga
     9901 aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt
     9961 atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt
    10021 tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat
    10081 tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag
    10141 ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt ctgttatact
    10201 agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg aaatttattc
    10261 aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat acatcgttta
    10321 aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat gaatattgaa
    10381 tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg gagaaattga
    10441 ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg aaagtttgca
    10501 cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct aatacttgat
    10561 ctagtagctc tttacctaac gttatcgctg agctccagat cgaatggctg tagtgacttg
    10621 ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac gttcgatctc
    10681 agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca tcctgttata
    10741 ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta attctgtgta
    10801 aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg cttgaaggcc
    10861 tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta tgataactcg
    10921 agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga taggaatctg
    10981 tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc cttctttttt
    11041 cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac aagaataaat
    11101 actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca ttctgatcct
    11161 cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta tcttctacat
    11221 acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg atatgtccta
    11281 attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac atttgtttat
    11341 caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat tcaatcttat
    11401 aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt attttttctt
    11461 tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag atcgtcatct
    11521 ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg tttctagatt
    11581 ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt gtgatttctc
    11641 agagtacaat ggatgtatgg a
//
LOCUS       DAYYKC010000090        11574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000090 DAYYKC010000000
VERSION     DAYYKC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.091"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..2380
                     /gene="acm"
                     /locus_tag="ACHBBW_002341"
     CDS             215..2380
                     /gene="acm"
                     /locus_tag="ACHBBW_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9153999.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2652..3137)
                     /locus_tag="ACHBBW_002342"
     CDS             complement(2652..3137)
                     /locus_tag="ACHBBW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9154000.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3227..3572
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3617..4258
                     /locus_tag="ACHBBW_002343"
     CDS             3617..4258
                     /locus_tag="ACHBBW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9154001.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4277..4912
                     /locus_tag="ACHBBW_002344"
     CDS             4277..4912
                     /locus_tag="ACHBBW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9154002.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4930..5745
                     /locus_tag="ACHBBW_002345"
     CDS             4930..5745
                     /locus_tag="ACHBBW_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9154003.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6064..7500
                     /locus_tag="ACHBBW_002346"
     CDS             6064..7500
                     /locus_tag="ACHBBW_002346"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9154004.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7689..10121
                     /locus_tag="ACHBBW_002347"
     CDS             7689..10121
                     /locus_tag="ACHBBW_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9154005.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10351..11418
                     /gene="rlmN"
                     /locus_tag="ACHBBW_002348"
     CDS             10351..11418
                     /gene="rlmN"
                     /locus_tag="ACHBBW_002348"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9154006.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         4037 a         1885 c         2481 g         3171 t
ORIGIN      
        1 aataaaatga atatatgcga aaaaataatt tacattagga aaaaccttgt aaaaacagtt
       61 ttttggcata tttatttttt ataaatattt gtaaaataac tatcaatttt ttagttaaaa
      121 gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga tatagtaggt
      181 tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa atattatttt
      241 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
      301 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
      361 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      421 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      481 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      541 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      601 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      661 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      721 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      781 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      841 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      901 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      961 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
     1021 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
     1081 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
     1141 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
     1201 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
     1261 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1321 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1381 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1441 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1501 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1561 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1621 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1681 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1741 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1801 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1861 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1921 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1981 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     2041 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     2101 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     2161 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     2221 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     2281 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga tagtaactat
     2341 ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata atattttttg
     2401 ttaaatatga ctgaaatcaa aagaataaaa ttccgattta taatcgaaaa aagaagcatt
     2461 tttatatatg cttctttttt cgtagaatca ataaatatgg atttggctaa ctatggaaga
     2521 atactcgtat tagttttcag cgttcatact agttgttaca ttgtttgatt ggccaaaaga
     2581 atttctgata tattttgtgc taaataaaga aaaagacgct tgaattggga aataagaaaa
     2641 ctactctatc attacaaatt ttcataaact ggcagatgtt ctaagtaaac aagtatcttt
     2701 tcaaagtcgg aaaaatccat aatcaacgta gtggtattga tatttggatg aaagcctacg
     2761 gtatcttgac gatcaatagc agcatcgatc acgacttgga tttttttctc tttgtcgtgc
     2821 atcaatgcta atggagtcac tgtacctgct ggaacgccta ggaggtcagg aagctgttct
     2881 tcagaaacaa aagataagcg cttttctttt aattgttcag ctagttgttt taaatcggcg
     2941 tgcttttcat caggcaagat gacaagatag atttgttttc cttttcttga tttcaataat
     3001 agatttttta cttgcggacc aggtagggaa aaagggacat ttttgactga cgtaatcgca
     3061 ggatgatcga ctttttgaaa agagataccc agttttttta gtagttcata agcttcttgt
     3121 tcggttgcac gactcatcgc gcatcaccct ccttttttta ttagtgtatc ataaaaaaga
     3181 gttgacaaat gaaaaataat catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa
     3241 aagaagagta gccgttaggt gtttttagag agtctctggt tggtggaaag aggcagcagt
     3301 taaccgtgaa acacatcttg gagtcgattt tctgaagcgt atagtaggaa aatccgggga
     3361 gcccgttaca gctgcggtcg ttgctagatg accagataag gctggctttt ccgtgaggag
     3421 tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg atcttcggat caaaggactt
     3481 tttgttttca tctgaacggc aacgactgtg tgaggttttt tctgcaagga aaaaataaac
     3541 agaggtggaa ccacgtataa tacgtcctct tggcatttgc caagaggatt tttttatttt
     3601 aaaggaggaa aagaagatgg agtatttgat tgaagttatg ccgcaattat tagtaggagc
     3661 actcacaaca ttgaaattat ttttatttac cttgattggc tcattaccat tagggattgt
     3721 tttagcatta ggattgtctt tgcattttaa gccgctacgc tacttgcttc aaacatatgt
     3781 atgggttatg cggggaacac cgctgttgtt gcaactgatt tttatttttt atgggttgcc
     3841 gacgattggg atcgtttttg atcgtttcga agcagcactc attgccttta tcttaaacta
     3901 tgcagcttat tttgcagaga tctttcgtgg agggatacaa tcgattcctc aaggtcaata
     3961 tgaagcagcg caagttttgc aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc
     4021 acaagtgaca aagattgttt taccttctgt agggaatgaa gttatcaact tggtcaaaga
     4081 ttcttccttg atctatatat taggtttagg tgacgtaatg cgtgctggga aaatcgcgat
     4141 ggaacgcgac gcgactttac tgcctttgct aggcgtggca atcatctatc ttacattgac
     4201 tggtattcta acaatcgtaa tgaaacaagt tgaaggaaaa ttaaattatt accgctaact
     4261 gataggagga agaaatatga ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa
     4321 aataatcaaa gatttgaatc taatcattcc agatgggaaa acattagcta tcgtaggtcc
     4381 ttccggtggc ggtaaaacca cgcttttacg tatcttggct ggcttagaaa gttctgacag
     4441 cggagaattt tatttagacg gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca
     4501 agttgtagga gtcgtattcc aagacttcca gctatttcca catctgaata tttttgaaaa
     4561 cattacctta gcaccaaaaa tggtattgaa acagacaaaa gaagaataca ccaaaaaagc
     4621 agaagaatta gcggcttcat tagggattag tgatttattg aatatctatc cttatcagct
     4681 ttctggcgga caaaaacagc gggtagcgat tgccagagca ttggctatga atccacgggt
     4741 attggcttat gatgaaccaa caagtgcttt agatcctgaa ttacgccagc aagtcgagcg
     4801 tttgatccta tcattgaaag aagatggggt aacccaaatt attgtcaccc atgatttagt
     4861 atttgcagaa aatgtcggtg atcaattact aaaagtttcc ccaattcatt aagaaaggag
     4921 aagatagaga tgaaaaaaac cagttttcta ttcgctttga tcgcaggtct tcttcttttt
     4981 gcaggctgtt cgaacaaaaa aacttcagca gataattggg cgactatcaa cgaagagaaa
     5041 aaaatcgtta taggtttaga cgatacattt gttcctatgg gatttagaga taaagacggg
     5101 aagctcacag gatttgatat tgatttagcc aaagcagtct ttgaacaata tgggattacg
     5161 gtagactttc agcccattga ctggtcaatg aaggaatttg aactgaacaa tggaacgatc
     5221 gatttgatat ggaacggcta ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca
     5281 aaaccttata tggaaaacga tcaagtgctg atcacaccta aagaatcagg aatcacaaac
     5341 tttggacaaa tgaataataa gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta
     5401 tttacgaaac aaccagaagt attaaaagag atagtcaaaa accaagatgc tgttttgtat
     5461 gatagtttta atgaagcatt gatcgatctg aaaagcagac gggtcgatgg tctgctgatg
     5521 gataaagtat acgcagacta ttatttgaaa caaagaaacg aactagaaga cttcaacata
     5581 acaaaaggtt cttatgagag tgaggatttt gcagttggtg cacgcaagtc agatagtaca
     5641 ttagtgaaaa aaatcaatga agccctagat caactagaga aaacaggtga attccaaaaa
     5701 atatccgata aatggtttgg cgaagatgtt acgccgagaa attaacaaat aggggactgt
     5761 gacaaaagac gtgtcacagt ccctttttcc gaataaacag tgttctcctg ccaactcctt
     5821 gtgactcgga gctgaatggc aggctcacaa cctcttggta aacggtgttc aaaagccagt
     5881 ttttcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc
     5941 ttactactcg aaactaagcg actttttcac aacctccttt acataatttt attttgttgt
     6001 ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa ggttatagga aaggaagaga
     6061 tgaatggaac acaaagaact tacctcattt ccaaaagatt ttctgtgggg ctctgcttct
     6121 gcagcgtatc aagtagaagg agcttggcaa gaagatggga aaggagaatc agtctgggat
     6181 cgatttgtac ggatacctgg aaagacattt aaaggaacga acggtgattt ggcagtagac
     6241 cactatcatc gttataaaga agatattgct ttaatgaaag aacaaggtct gaaagcttac
     6301 cgcttctctg tagcttggac acggattttt cctaatggac gaggagaagt caatcaagct
     6361 gggctcgctt tttatgagag attgatcgac gaattgattg aaaacgatat tgaacctatg
     6421 ttgactttat atcattggga tcttccacaa gcattgcaag atgaatataa tggttgggaa
     6481 tcacgtcaga tcattgaaga tttcactcat tatgcagaaa cattatttga ggcattccgc
     6541 gggaaagtcc attactggat tagtttgaat gaacaaaata tcttcacttc attaggttat
     6601 ttgttagcag cacatccgcc aggtgtgaca gacccgaaac gaatgtatga agttaaccat
     6661 attgctaatt tagcaaatgc atctgtcatc aataaatttc acgagttaga aatgcctgga
     6721 aaaatcggac caagctttgc gtatacgcca aattatccaa ttgacagtaa tcctgaaaat
     6781 gttctggctg ctgaaaatgc agaagacctg atggccaatt attggatgga tgtctatatg
     6841 tggggaaaat atccgattgc tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg
     6901 attgaagctg gcgatgcaga attgctagaa tctgctaaac cagattttct tgggatcaat
     6961 tactatcaaa cggcgacgaa tgcattcaat ccattaaacg gagttggtgc tggaaaaatg
     7021 aatacgacag gaaaaaaaag ttcatcagaa gaaactggag ttcctggaat gtataaaaaa
     7081 gttgagaatc catttgtcga acgaacaaat tgggactggg agattgaccc agaaggatta
     7141 aggatcggac tgcgtcgtat cacaagccgt taccgcatgc cggtactgat tacggaaaat
     7201 ggcttaggag agtatgacaa gttgacagaa gataaacaaa tccatgacga ctatcgaatc
     7261 aattacttac aaagccacgt caaagccatc aaagaagcaa tttcggatgg agcagaagtc
     7321 ctaggctatt gcacctggtc ttatacagat ttgcttagct ggctgaacgg ttatcaaaag
     7381 cgatatggat ttgtatatgt tgatcaagac gaaacacaag aaggatcttt ggaacgatat
     7441 aagaaagaca gttattactg gtaccaaaaa ctgattgaag aaaatggaga gaacctttag
     7501 ttcacatact taaaacaaga aggtacggct ggatatggcc gtgccttttc ttgataaaat
     7561 catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct
     7621 ttcaaacaaa actttagttc ccttactttt ttttgtataa tgaaacttga tgaaatgagg
     7681 gataataaat ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga
     7741 aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt
     7801 tatttgctgt cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct
     7861 ttcggatcat tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat
     7921 tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg
     7981 gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc
     8041 atactgggga accaaatatc atggggacga catgggattt gttagccagt gatataaaac
     8101 aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta
     8161 cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag
     8221 gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg
     8281 gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg
     8341 caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa
     8401 aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg
     8461 atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca
     8521 gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac
     8581 agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag
     8641 aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc
     8701 ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg
     8761 gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct
     8821 taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa
     8881 ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag
     8941 caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg
     9001 tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac
     9061 ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact
     9121 tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa
     9181 gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg
     9241 ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta
     9301 gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat
     9361 tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact
     9421 tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag
     9481 ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa
     9541 tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta
     9601 tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta
     9661 cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata
     9721 tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg
     9781 accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag
     9841 agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg
     9901 agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc
     9961 tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag
    10021 ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg
    10081 aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt
    10141 tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt
    10201 ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag
    10261 ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag
    10321 cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg
    10381 aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa
    10441 gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg
    10501 aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc
    10561 gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg
    10621 attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta
    10681 ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg
    10741 actgctggag aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat
    10801 cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac
    10861 gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct
    10921 cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac
    10981 ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg
    11041 atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat
    11101 ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat
    11161 agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat
    11221 gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa
    11281 gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt
    11341 aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa
    11401 aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt
    11461 tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat
    11521 aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca attt
//
LOCUS       DAYYKC010000091        11525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000091 DAYYKC010000000
VERSION     DAYYKC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.092"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..618
                     /gene="rpsN"
                     /locus_tag="ACHBBW_002349"
     CDS             349..618
                     /gene="rpsN"
                     /locus_tag="ACHBBW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9154007.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            633..812
                     /gene="rpmF"
                     /locus_tag="ACHBBW_002350"
     CDS             633..812
                     /gene="rpmF"
                     /locus_tag="ACHBBW_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9154008.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            844..1221
                     /locus_tag="ACHBBW_002351"
     CDS             844..1221
                     /locus_tag="ACHBBW_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154009.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1242..1391
                     /gene="rpmG"
                     /locus_tag="ACHBBW_002352"
     CDS             1242..1391
                     /gene="rpmG"
                     /locus_tag="ACHBBW_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9154010.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1415..2398
                     /locus_tag="ACHBBW_002353"
     CDS             1415..2398
                     /locus_tag="ACHBBW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9154011.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2428..2550
                     /locus_tag="ACHBBW_002354"
     CDS             2428..2550
                     /locus_tag="ACHBBW_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9154012.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2568..4091
                     /locus_tag="ACHBBW_002355"
     CDS             2568..4091
                     /locus_tag="ACHBBW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9154013.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4115..4354
                     /locus_tag="ACHBBW_002356"
     CDS             4115..4354
                     /locus_tag="ACHBBW_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154014.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4547..5008)
                     /locus_tag="ACHBBW_002357"
     CDS             complement(4547..5008)
                     /locus_tag="ACHBBW_002357"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9154015.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5203..5421)
                     /locus_tag="ACHBBW_002358"
     CDS             complement(5203..5421)
                     /locus_tag="ACHBBW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154016.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5539..7155
                     /locus_tag="ACHBBW_002359"
     CDS             5539..7155
                     /locus_tag="ACHBBW_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9154017.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7396..8736)
                     /locus_tag="ACHBBW_002360"
                     /pseudo
     CDS             complement(7396..8736)
                     /locus_tag="ACHBBW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9174..9320)
                     /locus_tag="ACHBBW_002361"
     CDS             complement(9174..9320)
                     /locus_tag="ACHBBW_002361"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154018.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9359..9553)
                     /locus_tag="ACHBBW_002362"
     CDS             complement(9359..9553)
                     /locus_tag="ACHBBW_002362"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9154019.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9463..9795)
                     /locus_tag="ACHBBW_002363"
     CDS             complement(9463..9795)
                     /locus_tag="ACHBBW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154020.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10309..10500)
                     /locus_tag="ACHBBW_002364"
     CDS             complement(10309..10500)
                     /locus_tag="ACHBBW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154021.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10594..10980)
                     /locus_tag="ACHBBW_002365"
     CDS             complement(10594..10980)
                     /locus_tag="ACHBBW_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9154022.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3836 a         1810 c         2208 g         3671 t
ORIGIN      
        1 aattggcgat aaaccagtta aacataaaag attgaataaa aagttatagt ttctttcata
       61 ttgccgtttt aagtagagga aaagctactt aaaacggtat ttgtgtttga ttggactaat
      121 aaaagtgaat tcttattttt cagagcctaa actgcatatt cttttaaatg atttgattat
      181 ctctatgtac cgataaattc gttttcaaat agttatctat tcatatgagt tttatttcat
      241 gttatttatt gttgacaaga gcaactcttg ttggtaaagt atcagtgtca aaacgtaacg
      301 attacgattt gaggtgtcta aatcaaaata aaaggagtat aaaggtatat ggcaaaaaag
      361 tcgaaaattg ctaaagcaaa gaaacaaatg gcgatgattg aaaagtatgc agataaacgt
      421 caggaattaa aagccgcagg agatcgcacc gctctcgcta aattgccaag agattcaaat
      481 ccgaatcgtc tgaggcttcg tgatcagacg gatggtcgtc caagagggta tatgcgaaag
      541 ttcggtatgt cccgtattaa gtttagagaa cttgcccatc aagggttgat tcctggagta
      601 aaaaaagcaa gttggtaaaa ggaggaaata tcatggcagt accagcaaga aaaacatcta
      661 aggcgaagaa acggttaaga agaactcatc aaactgtagt aaaaccagaa atttcatttg
      721 acgaagcgaa tggtgattat agacgtagcc atcatgtatc tttaaaaggg tactataatg
      781 gaaagaaagt aattaaagga gcttcaaaat agtttaatat ataactttta ggagggaatc
      841 aaaatggaaa ttatttaccc acctttagtt gaagaaggat taaagtatta tcttgaaacg
      901 acccagcaat cgctagataa atgcactttt tatcgttcga tggtagagag aggaattatt
      961 actgaaacgg gtttaccgac ccaacaggca attgaaaatg gattagtcaa agattactat
     1021 gaggaccaag gcttatcatt tgatgatttt ttaagaatct atccaatttt tgaagagtat
     1081 gacgaggagt tatttcaatg tatcgatggt tattgggaga tacctgttga tatgaaagaa
     1141 aatttagttt cacaattgga atctggcgag ttaacttttg aagatgcaca acaaattcaa
     1201 gcatatcttg aagatcgata agtattgaag gaggaagatt tatgcgtcaa aatattattt
     1261 tggaatgtgt cgaaactgga gaacgtctgt atctcaccag caaaaataaa cggaataacc
     1321 ctgaacggtt agaattaaaa aagtattcgc caaaattgcg cagacgggca atttttaaag
     1381 aagtgaaata gctaagtggt aaaaaggtga caaaatgggc ataccagtta caatcgtttc
     1441 tgggttttta ggggctggaa aaacaacgct aatcaatcaa gccttgcaag tctcacccta
     1501 ccccagggaa gaaattatta taattgaaaa tgagtttggt gaagttggga ttgatcacaa
     1561 attattagtt catagcgagg aacgggtgtt acaactaaat aatggatgta tgtgttgcag
     1621 tcttcgcagt gatttattag caatattgat ttctttactg gaaacaatag aagaacatca
     1681 acaaccaatt tcacaaatca tcattgaaac tacaggtata gcagatcccc aaccaatcat
     1741 tcaaacactt ttaacagcgc ctcaactaag aggaagagta tatatcgata gtttattgac
     1801 tgttattgat agtgacagtt ttgaacttat tgaaagagaa ccacaagccc ttaagcaact
     1861 tgtcatggca gatcgcattt ttgtgtctag aaaacaaaac ttgaatttat ctgcactaaa
     1921 aaaattacag agagaaatca aaagtattaa ccctttatcc gatattcgaa tgttcacaca
     1981 tagtgaaacg attcaaaaaa aggattttta taatttgaaa aaatttaatc atcccttagt
     2041 ttttgaaagt gattcatcag aaaatcacta taatggcaaa tactatcatc aacaccatca
     2101 agtacatgag tttaaatcaa ttctcttaac ttcagatcaa gacataaaaa aagatttttt
     2161 actccaatgg attgactggc tgattttaac caaccaaggg agcctgtatc gttttaaagg
     2221 attgattgct ttacaagagc aggattttat agttgccatg caagaggtaa acgaacaggt
     2281 taattttcaa tacaccactc gacaaaaaga ccaaaccact attattctaa tcggaaaaat
     2341 gttggatgaa gaaaggatca aacagagttt tgataaatta ctggcggaaa gcaaataatg
     2401 ataagtagtg attgggggta atttctaatg gagaaaacgg atatatctag tgcatatcga
     2461 cgacttaaaa gcccgaatat taaaactaga aaaagggcat taaaaattat caaagaacat
     2521 aaacgaaata aaatgaagaa attagcataa ttaaggaagg aaaaagaatg aagaaaatca
     2581 gtgtaggact tattgggata gcagctctag gattattggg agcttgctcc agcactaatg
     2641 atgcaaaagt atcgaatgat aaagatggaa aattagaaat tgtaacaaca ttctatccaa
     2701 tgtatgattt caccaaaaac atcgtcggcg atgaagcgaa cgtggattta atggttccag
     2761 cgggatcaga acctcatgac tatgagccct cagctaagga tatggcaaaa gctcatgatg
     2821 ctgatgtttt tgtatatcat aacgaaaaca tggaatcatg ggtaccaaaa gctaaagaaa
     2881 gttggaagaa agctgggcct aatgtagtag aaggaacaaa agacatgatt ttattgccag
     2941 gtagtgaaga agaagatcat gatcacggag aagaagatca ccatcatgaa ctagatcctc
     3001 atacgtgggt ttcacccaaa atggcaatta aggaagtttc aaatatcaag gatcagttag
     3061 ttaaattgta tccgaaaaaa gcaaaagtat ttgagacaaa tgctgaaaag tatttgacta
     3121 aactgaaacg gttggatgct gactatacca ctagtcttaa agaagcgaaa caaaaaagtt
     3181 ttgtgacgca acacgcagcc tttggatatt tagctttgga ttatggattg aaacaagttc
     3241 ctattgctgg actaagtcct gaagaagagc catcttctgg tcgattagct gaattaaaag
     3301 agtatgttaa aaaaaataag atcaattata tttactttga aaagaatgct aatgataaaa
     3361 ttgcaaaaac tttggccaac gaagctggga taaaattgga agtactgaat cctcttgaaa
     3421 gtcttacaaa agaacaaatg gataatggtg aagattatgt ttcagtaatg gaagacaatc
     3481 taaaagcgtt agaaaaaaca actatggttg ctggtgaaga ggtagttcca gaaaaagaag
     3541 cgaaggatga aaagacagtt gctaatgggt actttaaaga tgccgatgtg aaggatcgcg
     3601 aattgagtga ttatacaggc gaatggcaat ctgtttatcc gttgttaaaa gatggaattt
     3661 tggatgaagt tttcgattac aaagcaaaaa tcaataaaga catgacagct gccgagtaca
     3721 aagattatta tactactggc tataagacag atattgatac tatcaacatt aaagacaaca
     3781 ccattgattt tgtagtcaac ggtgaacatc atcaatacac atacaaatat gttggataca
     3841 aaattttgaa ttatgaaaaa ggaaaccgtg gtgttcgttt taattttgaa actgatgatg
     3901 caggtgctgg tcgttttaaa tatattcaat ttagtgatca tggaattgca ccaagtaagg
     3961 cagagcattt ccatattttc tttggtggag aaagtcaaga aaaactctat aatgaattgc
     4021 ataattggcc tactttctat ccagcttcat tgtctaaaca tgaaattgct caagaaatga
     4081 tggctcatta aaattcaatt tttggaggaa atacatggta agaaaagtat tattgtctat
     4141 ggattccaag aatattgatg cttatcggcg ttatcgattt gcaaagcaaa tgagattaaa
     4201 tgggagtaca ttagctgata ttgctgcaac tttacatgtt agtattgaca cagttcatag
     4261 agtattaaaa cgggatcctg attgttgttt tatcaaggtc aatactggaa gaagtagacg
     4321 tttagcaaaa cggcataata gcttggccaa ttaattgata ataccagagt agatcatttt
     4381 tcccttattt ggatttgatc tgctttttat tatgtcttga attgtcaaat caagcgcaac
     4441 actttatcaa agaagacttc ataaggtgtt ctccaatcta aacactttct aggtctgtta
     4501 ttcagttttt caatcatctg atttatcgtt gagttatcta ccgtagctat ctctttcgat
     4561 tttggcaagt attctcgaat gagtctattc gtattttaat ttgttcattg ttgccaaggg
     4621 catgaggatc tggaaaataa aagggcatat tattcaatgc ttctgtaact gcgaaatgat
     4681 tggagaattc ttttcctcta tctggagtga tcgttttgac cgcctcattt ggaatactgc
     4741 ttaaaagttc aatcattttc tcgctcacat aagcggcttt ctttctgggg accttctttc
     4801 ctgctaaaag gaagcatgat tttctgtctg tcaaagtcac taaacaagta ccaccagttt
     4861 tgccagctac aatatcggct ttccaatgac caaaatcact tctatcatct gcggattttg
     4921 gacggccatg gatcgaatga gtaaggcaaa tctttccacg agactcgaca tgattttttg
     4981 tatgtcttgt ttttcctcga tggtgcaatg aacggatcag gcctttattg ccgtgtgaga
     5041 ggggttctgt atcgaaaaga tcattgcaaa ttgcacgtta gatcgtgata aaactaattg
     5101 aaaagtccat atgttcatgc ttcaaacgat tcgagatttc ctctggcgac cactgaaaat
     5161 cgacaaataa atgacgaaca attgaccaaa ctcacggatc atttagaatg tgataacgtc
     5221 cgcatttttt tcttcgataa gcataatttt tttccgcttt ggaaggtgaa tagttttttt
     5281 cttgtttctt ctcagttcat gtaaaatagt aaacgggcta tgctgaatgt cgttgccaat
     5341 tatactaata gaaaatcctt cttcgtgaag cagaaagatt ctttctcgtt cgagtatagt
     5401 aagatgacga tactggctca taattatttt ccttctagga gtgttctcga caaacttata
     5461 ctagttggat atcgattatg actcattttt atgtgttgca cttaaagtgt aaattcaagg
     5521 tatagaaaga ggttccctat gaaaaaaata cctattacaa tcttaactgg ctatcttggt
     5581 tcaggaaaaa cgatacttct aaatcatatt ctgtacggtg atcacggaaa gaaaattgct
     5641 gtcattgtaa atgacatagg ggaagtaaat gttgatgggg tattagtcga caagaaaggt
     5701 tttaaaagaa ctggagaaaa acttgtttcg atgccgaatg gatgcatttg ttgtacatta
     5761 cgcgaagatt tgattgttga actagatcaa ttagtgcaat tttctgactt gaatcaaatt
     5821 gttattgaag cttcaggaat cagcaaacca attccaatta ttcaagcaat tatattagct
     5881 aaaaaccctt tagacattga tttgacccaa cgattagaga ttggtgccat tattatagta
     5941 gtggatgtaa aacgtttttg ggataatttc gaaacgggtg aaaagattca ggaaagaaaa
     6001 gctgaaggta atgtagttga agaagaacag gatattcgtg gcttgttaat tgatcaattc
     6061 gagtgttgta acatattact tttaaataat agtgacttag tgtcaaccaa gacgatttac
     6121 aaaattaacg gtctaattgc taagttacag tccaatgcca aaataattag gacaattaaa
     6181 ggtgaagtag acttatcgaa aattctaaat acaaatcttt ttaatataga tgagacttcc
     6241 tattcagtgg gctggttcaa agagttagag cttggctatc aaaatcatat tcctgaaaca
     6301 gaagaatatg gtatctcaag ttttatctat cgaaatcggt tactccttga cgcacaaaaa
     6361 tttgcccatc ttacggataa aaattttcct ggcaatatta caagagcaaa agggtttctt
     6421 tggtttgccc aacatagtga tttttcgacg atctttgaaa catcgggtag ctataaaagt
     6481 attgaatcat taaattattg ggtagctagt ttacctaaaa ttgagtgtca aatgattata
     6541 caaaaaaatc ccgaattgaa ggaagcctgg catccgttgt ttggtgaacg cattaataaa
     6601 ctagttttta ttggtatgaa gatggatcaa caatgtatta aggaacaact tgagggttgt
     6661 ttaactgatt ctcttaatgc agataatttc cgaaaaattt caccatttcc atggaaaaag
     6721 tctaatatgt ttagccagat atcgttaatg aaatttttaa gaaaagctgg tgtcaagata
     6781 ggcgatgtca taattattgg cattttgatt attacctctt ttattccctt atttgtattc
     6841 tctatagaaa aggagaatga aacaactgaa gatgctatca aatatgccat cgttcgaatt
     6901 gatggcaagg aaatggatcg cttcgatttg gataagatca atcataaact cgtgaattat
     6961 catcctgcga agggacaata taatattgtt gagattaaag atggaagaat tcgagttaaa
     7021 gaagataata gtcctgatca aatcgcggtt agaactggat ggatctcaaa acccggtcaa
     7081 acgagtattt gtttacccca taagttagtt attagtattg agaaaaaaga aagcacagat
     7141 tactacattt actaatctgg aaatagatga ggaggttttt aataagaaaa agatattatt
     7201 attaataata gcaggggctt tattaagtgt gttaagcgct tgtggacaag aaaaagaagc
     7261 ggtgacaaca ggcacaagta tctttaacgg gacctgcaat ggtttagtta acagtcggtt
     7321 agattactta ttttggctct tcgtcaatga gtgttggtga agaaattcaa tagaataatc
     7381 gagatactca cacggctaga cagctatctt gtctagccgt gttgttgttc tggctttagt
     7441 cagttgggta gtattcttag ttttcttaaa atattttcct cgatataaaa agattcgttc
     7501 tcggtagttt tgaaagtttc tatagccata ggcaattcgt tttagtactt tgatatggtt
     7561 attcaaacat tctatagggc cgttggaata gggaacgcgc aacgctcgtt tgatttctgt
     7621 ttggtatttc ttgaaggtcg tgaacactgg ttggtagtgc tctggcaact ccttgtagtc
     7681 ttcttttagg agcgcgacga attcaggaac atcttgtctt ctgataacgg aaagaaaatc
     7741 ctggtagact tcgtagcctc gctttaagga atcatcataa gataatagcc gatcgactat
     7801 ctctgcttct gtcagatgat ctcgaaatga cggacgccag tagcgatgct gacctttcaa
     7861 ttcccacgca ttcttttgaa gtagtttcca gtatttcttt aagtgcttcg accgacgatc
     7921 ccctttttta aaggtcttca ttacttgaat tcggtggttg gtaaaagccc gaccaatgtg
     7981 ttggctaata tgaaagcgat cggcgacgat tcgggcattc ggaaaacact ctttcaccaa
     8041 tgtgcgatag ggtgtataga aatctgaaac cactagacgc acttgttcac ggacttttat
     8101 agggtagcgc aagaaatagt cgcgtaaagg attgagttgc cgattttcca caatatcaat
     8161 cagttgatgg gtcttgccgt ccatcatgac aaagctcatg gctcccgaga catttttcac
     8221 tgatttgaat tcatcgaaac acaccacttc tggtaacagc tggctcaaag aaatggtttt
     8281 aggccgaagg ctgcgaagta ttcgaatgac agtggtcgga gaaatgtgct tcatacgtgc
     8341 gatcaaagac atagaaagag gttcgcttaa tagctcaaga atggcttgtt tgactctgcg
     8401 agcaatggag cagcgacgat cactaactga atcttctgct aaaaaagtct ttccacagtg
     8461 tttacatttg aagcgctgtt tgttaatccg cagaatggtc ttgtagttac tgacatcatt
     8521 caataagatg gatacttttt tccagcccta tttaatgatc gtttgttcat tttgatagtg
     8581 gcgatgaaag cattcctccg gttgataggt caatgtgcca tcaaaaacaa aacatgtttc
     8641 gtcattgatt cgctctttgc gaaaggcatc ttcatgaaag ataagattta ggtctagaat
     8701 atctagtaac tctttggtaa actctgaata ggacatcttg aaacactcct ttgaatggtt
     8761 ttcgtcgact ttattctaaa ggattttaag gtgtctgttt gcgtttatta gaaaaaagaa
     8821 aaagtattgg caacagaagg cgtcaccaac actagatatt atagatccct tattttcttc
     8881 gcttcaaatt gtcaaggagt catcataaat tttgtatgga ttcttttgtt tgggtagtag
     8941 tcttctaaat aatcttggat taacaccttc atctctattt tacttttata tttgttggag
     9001 tcgattaatt ctcatttgat tgatcgatga aacgcttcaa ttactgcatt gtcaacagga
     9061 gtcccagttt ggctcactga atgtgtgatg tggtggtcgt tcaacagctc ttgataaata
     9121 aaagaccgat gctggctacc tctatcggaa tgaattaccg taggcaattc tccttaataa
     9181 atttgtaatg gacggacgac aatctctttt ttcatgtgtg aatctaccat atagtaacgt
     9241 agaatacgag ttgctaagtc aatataggtg gacaaataga ggcgaccgtc attacatgga
     9301 atgtaagtaa tatccatcac ccaaacagca tcaatttggt tctgttcaaa ttatcgattc
     9361 aacatttttt taccatttct ttgattgttt tttctctacc atgagaagat ttgcgtcgat
     9421 gaaatcctct tgtgcataaa ttcatttcac gcataagaac agctacaaca cgtttattga
     9481 tttcgattcc ttcatcgtaa agtttttgag tgatccctgg agatccgtat cgatctttgt
     9541 gttcgtagaa cactcgtctt cctcttttag tcgtttattt tcctgttcaa gaatgtagaa
     9601 atctgcttta tcaggtaatc tttttccttg tccagcaaaa gcatttttcc cgtatcgacg
     9661 atattctgaa acccatcggg aaattgttgg acgagagata ttgtattctt cagcaatttt
     9721 cttggtagtt acttttttct caaggatatc ctgtgaaatc tttagtttaa atttacgatc
     9781 atatgttttt ctcaatagag ttcattcctt ccggtaacgt aactgatagt caagaaaagt
     9841 gtatcatagc ccaacttagt atcagggttt gtcaactaaa ctgtggaagt tgatagataa
     9901 gagtttttaa caaccaaaat tctcttgact atttttgaaa tcagacaaat tttgattgga
     9961 cacatagttg aataaatcta ccgtcgttac ggaaggtaaa tcgcatgctt ttcaattcta
    10021 gaggagaagt cctcgttgaa gtaccatagc ccagttctgg attcgacctc ctgcccactt
    10081 catacgtaaa taaagtaatt ttaggagtga gactgtgaca aaagttattt ttaagctaaa
    10141 aacgaattaa aaaacgttga taattaaaat cattcgtttt agattctatg aatcattata
    10201 gaacgctgta aaatcgatac tttataatta taaagaagat agtttcaaat ttattgttcg
    10261 aaatttgaaa ctatcttttt acttatgtca cagactccct tagttgagtt attctttaat
    10321 ttcgaatttc gagatagcta gatatattgc aagcatacat tcttgataac aatcttgatc
    10381 atattttcca gttctccagg attgcttatg aatcaaagag ctaaatcttt tgagaattgc
    10441 ctcaacagct tcttcatttc cagcctttgc taaaatgaaa tcttctttta aagaattcat
    10501 aaatctacct tcttcccatg gacttataat ctatttcttt gggaaggagg agaacgagca
    10561 aagaagcata caaaattagt tattatgcag atattaaacc agcatttttt gaattttttt
    10621 gtaaagtcga tgttttaaat ttgtcgcacc ctgtcttgta atccccagaa cctttccaat
    10681 ttctttatca gttttatcaa agataaattt ttcgataaga aaaatttgtt caggttcttt
    10741 caacaaagga aggatttttg ctaattttat atcttcaaca aggagagtca catttaaaac
    10801 attaatcggt tgtttaaatt ttatagtatc tttagtaaac aaatatggtg ggagttgatc
    10861 aagttgttca tgtttttgaa tttcttcttg ttttttataa taagtattag cggtgttcaa
    10921 aatgaccttt tcaaagaatc gaatcaactg ggcatttacg cgttcgtcgt tatctgacat
    10981 attgcacacc tcctccatta agatttaaag ggaaaagagg caaaaaacac gaaaagaagc
    11041 taatgcaatt atgctgcgct gcgaataact tctataagct cacatgcatt gataggactg
    11101 ctgatgttta ttttcaaaaa tgttttttta gttgataatg gatagttatc tttatactta
    11161 gaaaagaaaa ttcatatatc ttcttaaata tcaaacatgt ccttatggct aagaaaatgt
    11221 cttgagcttt ttacgtataa taaatctttt ctaaaacctc cttttggata atattcttat
    11281 tcgtgtatag attgtatttt ttatttcttc ccccttcata attaacaact agaaaagagt
    11341 tcgaatagac acgtatttga acgaaaattt ttattggatt cccaaattgg aatatttaga
    11401 aacgaaaaat tcctattatc tgtatagtgc ttagataata acggatcata ataattattg
    11461 tctttgaact agatatatgt aaatagtatt catcatgatg aaaaatgatg agttctcggt
    11521 gataa
//
LOCUS       DAYYKC010000092        11254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000092 DAYYKC010000000
VERSION     DAYYKC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.093"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHBBW_002366"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHBBW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9154023.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHBBW_002367"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHBBW_002367"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9154024.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHBBW_002368"
     CDS             complement(2169..2906)
                     /locus_tag="ACHBBW_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9154025.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHBBW_002369"
     CDS             complement(2997..3524)
                     /locus_tag="ACHBBW_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154026.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHBBW_002370"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHBBW_002370"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9154027.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHBBW_002371"
     CDS             complement(4401..4886)
                     /locus_tag="ACHBBW_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9154028.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHBBW_002372"
     CDS             complement(5039..5236)
                     /locus_tag="ACHBBW_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154029.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHBBW_002373"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHBBW_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9154030.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHBBW_002374"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHBBW_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9154031.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACHBBW_002375"
     CDS             9211..9423
                     /locus_tag="ACHBBW_002375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9154032.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACHBBW_002376"
     CDS             9425..11104
                     /locus_tag="ACHBBW_002376"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9154033.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DAYYKC010000093        11222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000093 DAYYKC010000000
VERSION     DAYYKC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.094"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..1443
                     /gene="lysA"
                     /locus_tag="ACHBBW_002377"
     CDS             184..1443
                     /gene="lysA"
                     /locus_tag="ACHBBW_002377"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9154034.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1430..2230
                     /gene="dapF"
                     /locus_tag="ACHBBW_002378"
     CDS             1430..2230
                     /gene="dapF"
                     /locus_tag="ACHBBW_002378"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9154035.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2233..3114
                     /gene="dapA"
                     /locus_tag="ACHBBW_002379"
     CDS             2233..3114
                     /gene="dapA"
                     /locus_tag="ACHBBW_002379"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9154036.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3111..3827
                     /gene="dapB"
                     /locus_tag="ACHBBW_002380"
     CDS             3111..3827
                     /gene="dapB"
                     /locus_tag="ACHBBW_002380"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9154037.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3833..4528
                     /gene="dapD"
                     /locus_tag="ACHBBW_002381"
     CDS             3833..4528
                     /gene="dapD"
                     /locus_tag="ACHBBW_002381"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9154038.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4544..5545
                     /locus_tag="ACHBBW_002382"
     CDS             4544..5545
                     /locus_tag="ACHBBW_002382"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9154039.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5542..6903
                     /locus_tag="ACHBBW_002383"
     CDS             5542..6903
                     /locus_tag="ACHBBW_002383"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9154040.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6863..8014
                     /locus_tag="ACHBBW_002384"
     CDS             6863..8014
                     /locus_tag="ACHBBW_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9154041.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7989..9158
                     /locus_tag="ACHBBW_002385"
     CDS             7989..9158
                     /locus_tag="ACHBBW_002385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9154042.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9333..10712
                     /locus_tag="ACHBBW_002386"
     CDS             9333..10712
                     /locus_tag="ACHBBW_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9154043.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10993..11222
                     /locus_tag="ACHBBW_002387"
                     /pseudo
     CDS             10993..11222
                     /locus_tag="ACHBBW_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3735 a         1955 c         2503 g         3029 t
ORIGIN      
        1 tagcttctaa tagttcgggt attttgtttt tataaccatt tttattctta tttttaaaaa
       61 aatatttcga aatatactag acaattcaga aaattttgtt taatattatt ccaataaaat
      121 tcaaatcttt atcaaatgaa attccaataa aaacggaaaa aaagtgaaaa gaggaatgaa
      181 aaaatgacag aattgataat tggcggagtc acagcaagca cattggcaga aaagtataag
      241 acacctctgt atgtgtatga tgaagaaaag atgagacaaa cgatgctgtc attcaagcga
      301 ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg cctctaaagc attccaaacg
      361 attgagatgc tgcatttagt tcgtgaatat ggattcgggc tagatgttgt cagtggtggg
      421 gaaatctata ccgctttgca agctgatttt cctagagata ctatctactt ccatggtaac
      481 aacaagacgc cagaggaact gatctatgca cttgaaaatg atttgttgca tattgttgcc
      541 gataatttga tggaagttga attattagca gaattaagcc aacgctattt aaaaaaaatc
      601 tgtatcatgc ttcggttgaa tgtcggagta gaagcacata ctcatgaata tatcgtcact
      661 gcgcatattg attcaaaatt cggcatgagc tatgaaagta aagagtgcca acaagccctt
      721 cagttgataa aagaaagcag ctatctagag ttagaaggat ttcatgctca tatcgggtca
      781 caaatttttg atgttactgc ttggttagct gaaatcgaca aattgacgaa ttacttaacc
      841 gattttgacc aacctctttc cttgaatctt ggcggagggt ttgggattcg ttatacacaa
      901 aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac tgattgaata tacggaagaa
      961 gctttggaaa aacgaaaact gaccattcgt caattactga tcgaaccggg tagaagtatt
     1021 gttggagaag ctggtacaac actatacacg attggtttca tcaaaaagac accacataaa
     1081 aaatattact ttgtagatgg cgggatgaca gataatatcc gtccagctct gtatcaggca
     1141 gaatacgatt gtgatttagc agcaaaactg tcggaagaaa aaacagagaa agtgacgatt
     1201 gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag aagcttattt gccgaaagca
     1261 gaatcgggtg atcttttagc tgtttattcc acgggagctt atggatactc gatgagcagc
     1321 aactacaatc gagcgacacg accagcagtt gtttttgtca ataatggcca atcaagactg
     1381 attgttcgca gacaagcatt ttctgatcta ctaagggggg aagtttccta tgatccttca
     1441 taaatataat ggctgcggaa atgattttat cttattagat tacgcagaag acacagatta
     1501 tagtcaatta gccgcttctt tatgcacaaa agaaaaatat gatatagacg gattgatcgc
     1561 cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa gatggcagcc gcgcgccgat
     1621 gtgcggaaat ggcattcgct gttttgctcg atacgtaaat gatcagaaca tgatgaatga
     1681 acaggaattc aacgtagaga cattagcagg cacaagaaaa attacggtgt tggatccctc
     1741 acctttttat tgcagcgtag atatgggagt gccagatgat tctccggaaa aaatcaaagc
     1801 taatcaaact tatccaataa aaaaccagga aatagagatc aatggtgaaa aagtaacgat
     1861 cactagttta tttttaggaa cgattcatac cgttgttttt gttgaaaatg cgcgtgagga
     1921 agtctggaga aaaagaggtg aactgatctg tcatcatcct ttgttcaaag aaaaaaccaa
     1981 tgtcaacttt gtagaagtag tcaatgaaca agaattgatc gtccgtacct atgaaagagg
     2041 agtaggttgg acacttgctt gcggtacagg ttgctgtgcc tcttatgtgg ttgctaagga
     2101 aactgggaaa atttcagcag atcaggtagt tgttcacttg gaacaaggag acttgatgat
     2161 ctctggtgag gatgcgatac aaatggctgg cccagcagtt catgaatgga caagaatatt
     2221 ggaggaatga agatgataaa aggttcgatc gtggcgctga tcacgccgat gaatgaagag
     2281 ggatcagtag attatgctgg tttggaaaaa ttgatccaat tccatttaga tgaacaaaca
     2341 gatggattat tagttttagg tacaacagga gaatcttcta cgctcacaca atctgaggag
     2401 gagcagatcc ttcaactgac agtaaagaaa gtggctggcc gtgtgccggt gattgcagga
     2461 gctggaacga ataatacgaa agaaacgatt gaaaaagcga agcattttgc ttctttgggg
     2521 gcagacgcac ttttggtcat caccccttac tacaacaaaa caagtgatgc aggactagct
     2581 gctcatttta cagcaattgc agaagcctca ccgattccgc ttattttgta taatgttcct
     2641 tctagaacag gcatgtcgat tccaatccat gtattagtaa atttagcaga acatcctaat
     2701 atcattggtt tgaaagaagc ctcaggagat atggcttatg tgatggatgc cgctcggttg
     2761 attggagaag aattcttcct ttacagtgga aatgatgatt tgatcctacc agtaatgagt
     2821 gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc aacctaaaat agtccatgaa
     2881 ttagtaaaag atacgcagga cggaagatgg cagcaagcaa aagaaaagca attaaatgca
     2941 cttgaactga tccatgcctt gttcagtgag accaatccta ttcctgtcaa agcagccatg
     3001 tcattattag acttaccttc cggacctcta cgcctgcctt tagtttctct ttcaaaagag
     3061 aaaaagaaac aattagctca gcttttgtta aaagaaagga cggttgaaaa atgaaaatcg
     3121 gactgattgg cagtggacag atgggaaata gagtcgaaga agttgcacaa gaaagaggag
     3181 atacggtcct tcttttacgg acggcaccta aagaagcaac tacgcttgct tttacggaac
     3241 tgccagaact gcttattgat ttttctcatc cagataattt agagaaaatc ctttcgtata
     3301 gcttatcata tcaacttccg ttagtcattg gcacgacagg ttatacacaa tcacagttcc
     3361 aagaaataaa agaacatgcc aaacatgtac ctgtgatgta tagtgctaat ttttcgttcg
     3421 gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc aatgttgcaa ggctggcaga
     3481 tcgaactgat tgaaaaacat catagtcgta aaaaagatgc accatctggt actgcaaata
     3541 tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc cgtatacaac tggcaaggga
     3601 aaaaaagaga agaaaaccaa attggtgtac acagtattcg tgcaggttct ttacctggtg
     3661 aacatgatgt attgtttgct gctactgatg aaatatttac gatcaaacat gaatcttttt
     3721 ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga ctggttgaaa gatcagtcac
     3781 caggttatta caaattagaa gatttattaa tggacaaagg ggtgtaaaaa acgtggaagc
     3841 tcaagaaatc atccgctaca ttcaaacggc atctaaaaag acgcctgtga aagtctatct
     3901 gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt tttggtgaag gaagcagctt
     3961 cgttgttttt ggtgactatg agacgattgc tccggtttta gaagctgaat cagaaaaaat
     4021 cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca gttcctttgc tagatattaa
     4081 aaaaattaat gctcgtatcg aaccaggtgc tattatcaga gatcaagtgt caatcggcaa
     4141 caatgccgtg atcatgatgg gagcgattat caatattggt gcagtaatcg gtgaaaacac
     4201 gatgattgat atgggtgctg tcttaggtgg acgagcgaca gtgggcaaaa actgccatat
     4261 tggtgcagga gctgtcttag caggtgtgat cgaaccagca tcggccaaac cggttatcgt
     4321 agaagacggc gtactggtag gtgcgaatgc agtgattgtt gaaggtgtcc atatcggaaa
     4381 agatgcagtc gtggcagcag gagcagtggt tttagaagat gtggcagcag aaactgttgt
     4441 cggtggaata cctgcacgtg tattaaaaat aagtgatgat cagacaaaag aaaatacagc
     4501 attgattgct gcgttacgcg aattatagag gagatggaga aaaatgcgcg aaatagatac
     4561 ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg ctcaaagtgt tagaagaata
     4621 tgattttcca gtaggacgat tgaaattatt agcttcaaaa cgttcagccg gtaaagtttg
     4681 tgtattcaag aaaaagaaac acactgtaca ggaattgaca gaaacttcat ttgaaggata
     4741 tgatttagcg ctattttctg caggtgcttc tgtatccaaa gagtttgcgc caatcgcacg
     4801 aagtcaggga ctatttgtca tagataattc ctcagcatgg cgggaagatt cggatattcc
     4861 attaatcgtt ccagaggtca atctggaaga ctatacattg aatcggctga ttgcaaatcc
     4921 aaattgttca accattcaag cggtacttcc gcttaaagct ttacaagaag cctttggtat
     4981 ccagcgagtc aattattcga cctatcaagc agtttctggt tccgggcaaa aaggcattga
     5041 agatcttcat cgtacgcaaa atgacgagga accagttttg tatccttata atattagcca
     5101 aacggtgatt ccggaaatcg atcagccttt ggaaaatggg tatacaaaag aagaacagaa
     5161 aatgataaat gagacccgta agattctgca tcagcctgct ttgccagtat cagcaacctg
     5221 cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg gcagttcaac tgcaaaaaga
     5281 gtttacgacg gaagaaatta gagagtgtct cagccagttt ccagggattc aattagaaga
     5341 tgatctagca gcacataaat atccgacgag cattcttgct agaggaacag atatggttta
     5401 tgttgggagg atcagaaaag acacttcttt agagaacgga ttgttgttct atacgactgc
     5461 cgataacata cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag ctttaatgca
     5521 gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt cggcggaaca
     5581 tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa ttgttctttc agatcccaat
     5641 cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt cagatgatga caaagtaaca
     5701 gacttacttt atttgattta tgcttacctt cagcatcatg ttgattggac acccctttgg
     5761 aaaaggattt gtgatcgttt cattcaagta agagatgttc ttgccctgca gttacctatt
     5821 gaagatgaac tagcacgcat acagaaacaa ctggaagacg aaaagataac tgaagattat
     5881 ttagtaagta gaggtgaata tctgactgct caactgatgg ctgagtatct tggttaccag
     5941 tttattgacg caaaagacct gattttcttt gactatgatg ggaaaatcga tcaggaaaaa
     6001 acagcacagg aaatcacaaa agcatttaaa agatatccga aaatggttgt tccagggttt
     6061 tacggggcga accctgcagg aaaagaaaaa ttattgggcc gtgggggaag cgacattacc
     6121 ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg agaattggac ggatgtttca
     6181 ggaatcatga tggctgatcc gcgaatcatc gatcatccta aaatcatcga ggaaatctct
     6241 tttagagaac tgagagagat ggcttatatg ggggctaacg ttcttcatga agcagctgtt
     6301 ttcccagttc aagaggctga tatccccatc caaataaaaa ataccaatga tcctttcgct
     6361 caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa atggactgac aggaatagct
     6421 ggacgcaagg attttctttc catcacttta ttcaaacgtc atatgtccga tgaaatcggg
     6481 tttatctgga aagcaatgag catttttgct aaacatgaga tatcaatcga acatattcct
     6541 tcagggatcg acaatatcgg agtgatcgtc tcagcagaag cgattgccga taagttattt
     6601 ctcatcacga aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt gatcgaagac
     6661 ttagctttga tctctgtcgt tgggggtccg cataaagaat tgattggatt atctgggaaa
     6721 gtgttatcga tattgaacaa attagaaatt cgcacatcga tcttatcaca aggagcacag
     6781 gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg aaacagtagt gaagggtatc
     6841 tatgaaggga tggtgaatac ggatgctgaa agaatatcga atggcactcc atcaaattcc
     6901 tgaaatcggg tttcatgaat tcaagacaaa aaaatattta tatgaacaag taaaaaacag
     6961 cgaggccgtt atccatgaaa tcggcgaaac aggactactc ctttattttg ataatcagca
     7021 agaagaaacg attgcttttc gaacagatat cgatgcactg ccagtcaaag aagaaacgaa
     7081 tctctctttt gcttctaagc atgaaggtta tatgcatgca tgtggtcatg acggacatat
     7141 ggcgatgctt ttagggttga ctgactttat aactaaacac attgaggatc ttatttataa
     7201 tgtagtactg atctatcagc catcagaaga aatttccggt ggcgcacaaa gcgttattca
     7261 gtcaggattg ttggataaat atgaagtaca agcaatattt ggctttcatc tatggcctgg
     7321 actagctaag ggagaagttt tctctcgacc aggacctttg atggcacaaa gcagtgaaac
     7381 agacattatc attaaaggcc agtctgcaca tattgcttct agtgaaaaag gaatcgatag
     7441 tctggaggca gctgtacgat ttttgaagaa tgtttatgat tttgaccggc aattaccggc
     7501 agagtggaat catctaatga aatttggcca aatccaaggt ggaacgatcc gaaatgtttt
     7561 agctggagaa gttattgtat ccggaagtat ccgttcttac agttcagata tgcaaaatta
     7621 tttgaagagg ggattagacc atttagcaga acagtttcac tctacagcta ctatttcttt
     7681 tcgctataat gacggctatc cagctgtaat caaccataga ggcttatatg cagcactgtt
     7741 agattcaaac aaactacatg aattagatgc tccagttctt caagccgaag attttggcat
     7801 atatacgcaa caatatccat gtgtattttt ctttttaggt gtcggtgata cccctgcttt
     7861 acatgatccg acgttcgatt ttgatatgga tgttttagaa aatggtgtag aatggtacaa
     7921 aacgattctt tttacagaaa atcttcttgt aaaaatcgat agcagttcaa aagaggagcg
     7981 agtaaataat gatgaattta tccaatcgat ttagtcctag gctttctaga atagaagtat
     8041 caaaaatacg acaattcgat cagcaaatat cgtcgatacc agatgtgatc aaactcaccc
     8101 tgggagaacc ggattttcca actcctgaac atgtcaagca agcaggaata gcagcgattg
     8161 aagaagactt ttctcactat accggaatga gaggattaga ggaactgcgg gaagcggctt
     8221 gtatatttca gcaacagcgt tatggattaa cttatgatcc gcaaacggaa gttttgacga
     8281 cagttggtgc aacggaagcg attgcctctg cgttgttatc tgttttggaa gaaggagaca
     8341 aggttcttat tccagcgcct gcttattcag gatatcagcc attagtggaa ctagctggtg
     8401 ctgaattgat cccgatagat acttctgaca cagggtttgt ctgtcagccg gaacaatttg
     8461 aacgagcatt tgaacaatat ggatcagctg tcaaagcagt cattttaaac tatcctaaca
     8521 atcctactgg gacgacattg tctgcaaaac aattgaaagc aatcgctgag gttttaaaaa
     8581 aatatccggt atttgttata agtgatgaag tatatgctga attgacttat agtggaacgc
     8641 atatgtccat agctacttat cttccagaac aaacaatcgt gataagcggg ttatccaagt
     8701 cacatgcgat gactggttgg cgagtgggct ttatttttgc tcaaaaagcc cttattgacg
     8761 aactaattaa agtccatcaa tatctggtga ctgccgctac aacgatgtcg caaaaagctg
     8821 cggctgaagc gctaataaat ggggtgaaag atagcgagaa tatgaaagag caatatcaaa
     8881 cacgaagaga ctatctggtc cagcaattgg cgcctttagg atttgaagtg acccagccaa
     8941 acggtgcatt ttacttgttt tgcaaactcc cagctgccat tcaaactgac tcttggcagt
     9001 tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat cccaggaatt gcatttggcc
     9061 cagaaggaga aggttatatc cggatttctt atgctagcag catggaaaaa ttacatgagg
     9121 cctgcaaacg gatcaaagac tttttaacag aacagtgatt catagatttt atcaaaaaaa
     9181 caatcgttaa ttttgcggta tattctgcaa ggagacgatt gttttttaaa tttagttcaa
     9241 atgaacgcgt ttcttttgat tcataactaa tgtaatgtta agtttaaatt gtttagaatg
     9301 attttaaaag aatattcaag gaggtttcat caatggaaaa aatcaaagtt gtcattgtag
     9361 gtgcatctca tggagggcat caatcgatct tggaactatt gacacgatac ggtgaaaatg
     9421 tagatatcac gctatttgaa gcaggagact atatttcctt catgtcatgt ggtatggagc
     9481 tgtatttaga agacaaggta acagatgtaa atgatgtacg taatttcaga ccagaaaact
     9541 ttccgcaatc caatgttcat attttaaata atcacgaagt aaaaacgatc gatgccgata
     9601 aaaaaacagt aacggttact agcgcatcag ataacgaaac aaaagaatat ccttatgaca
     9661 aattgatttt gagttctggt gtcaaaccaa acaatctgcc agtaccagga gcagatctag
     9721 aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa aaattaaatg
     9781 atccggctat taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc gaagcagcag
     9841 aagcaagccg aaaagctggg aaagaagtcg tattgcttga tgtgatcgat agacctttag
     9901 gcacttattt agatgctgag atgacagata ttttagaaca acacctaaat gaaaacggta
     9961 ttgaagtttt gacaaatgca aaaatcgaag cttttactgg taacggaaaa gttgaggcaa
    10021 tcaaaacaag tgaaaaagaa atcccagcag atctagtcat tcaagctgcc ggtgtgaagg
    10081 caaatacaga atggttaaaa gggatcgtcg acttagatga acgtggctgg atccaaacaa
    10141 atgaatactt gcaaacgaat cttccagatg tgtatgcagt aggagatgct acacttgctt
    10201 attctatccc agcacgcaaa aaaatgccga tcgctttagc gactgttgca cgtagagaag
    10261 cacgttatgt agtaaaacat ctttttgaag aagtaccaaa caaaccgttc ggcggagtcg
    10321 taggttcctc tgcattaagt gtctttgact atcattttgc agcaagcgga ttgaatagct
    10381 ttacagcagc aaaagcagga gtagatgtac gagtgtctta ttatgaagat acgttacgac
    10441 caaaatatgt accagaagaa caaggaaatc caaaagtgtc catccaatta ttctcggatc
    10501 caatgaccca tcaattgcta ggaggagcag tattatctac ctatgatgtt acaggtcaag
    10561 gaaatgtatt ggcactagcg atccaaaaca aactgacttt agaagaacta gcagaagctg
    10621 acttcttctt ccagccaggc tttgaccgac aatggagcat gttgaatcta gccgctcaac
    10681 aagcgttagg agaagaacca tttgtagaat aagaaaacga aatatttgaa aagaggttgt
    10741 gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
    10801 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attttcaaaa
    10861 aagggactgt gacacgattt gtgtaccgac ccccaaaagt tagaccaaaa aatctaactt
    10921 ttggaggtcg gtatttttta tggttaaata tacttttgaa cttaaattaa agatcgttca
    10981 tgattatttg gatggcaaag gcgattggac cgtaaaaatc cagatcaaga gattgaagcg
    11041 gaaatgctta aaattcggga gaagcacaag gattatggct gtttacggat gaccaatgaa
    11101 ctaagaaacc gcggtttctc tatcaataaa aagaaagttc agcgcttgat caagaagcta
    11161 ggaataaaag taacggcata cacgcgtaaa tcacgtcgct acaattctta tcgaggaaaa
    11221 gt
//
LOCUS       DAYYKC010000094        10902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000094 DAYYKC010000000
VERSION     DAYYKC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.095"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1333)
                     /locus_tag="ACHBBW_002388"
     CDS             complement(71..1333)
                     /locus_tag="ACHBBW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154044.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1326..2225)
                     /locus_tag="ACHBBW_002389"
     CDS             complement(1326..2225)
                     /locus_tag="ACHBBW_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154045.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="ACHBBW_002390"
     CDS             complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="ACHBBW_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9154046.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="ACHBBW_002391"
     CDS             complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="ACHBBW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9154047.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4731..7091)
                     /locus_tag="ACHBBW_002392"
     CDS             complement(4731..7091)
                     /locus_tag="ACHBBW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9154048.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7206..7754)
                     /locus_tag="ACHBBW_002393"
     CDS             complement(7206..7754)
                     /locus_tag="ACHBBW_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9154049.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7812..8249)
                     /gene="zapA"
                     /locus_tag="ACHBBW_002394"
     CDS             complement(7812..8249)
                     /gene="zapA"
                     /locus_tag="ACHBBW_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9154050.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8451..9368
                     /gene="rnhC"
                     /locus_tag="ACHBBW_002395"
     CDS             8451..9368
                     /gene="rnhC"
                     /locus_tag="ACHBBW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9154051.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9746..10657)
                     /locus_tag="ACHBBW_002396"
     CDS             complement(9746..10657)
                     /locus_tag="ACHBBW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9154052.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10710..>10902)
                     /locus_tag="ACHBBW_002397"
     CDS             complement(10710..>10902)
                     /locus_tag="ACHBBW_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154053.1"
                     /translation="RKCFNLITKKLIVCRMKETIYWVDESPRIVKRRFQDMPKMKKII
                     LAVMLFSTVISFTAPFLLT"
BASE COUNT         3081 a         2334 c         1829 g         3658 t
ORIGIN      
        1 aaaataggtc tatacaaaaa gaagctggga acaatagatg tccagcttcc ttctgtattt
       61 taggattgtt ttatgctttt tttcgttcat tcctcataat tgaaatcgat tgtttcaatg
      121 aactccataa tccgccttcg ctataaacga gtacattcga tttgtacatg tttgcagaca
      181 agatcatcaa taagaatgtt acggctacaa gtaccaacaa ggaaatcaat gctccaccca
      241 taccgactgt ctcatttgct aagcgaatcg gcataatata tgaagataag aatggaatat
      301 acgaagtgat ccgaataacg atattgtttg gatctgaagc tcctagtatc aatccaagca
      361 tgtatccgcc tagagataga taagtcactg gcaaaattgc ttttgctgta tcttctgctt
      421 tgttgaccaa agagccacat aaagctgcta aaacagaaaa aatcaaaatg ccaaagatca
      481 tgaatagaag agaataccac aagaatggtc caatcaagtt atccaatgag ataccgtcta
      541 atatattttt gaccatttcc atatctttga attgactgta tcctatacca aaaataatac
      601 cgtataatgc catttgcgtt aatgcaacaa gtaaaacacc tgttaatttt ccgtaaaaat
      661 gcttttgtgc agtcgtactg gacaggatca cttccatgat ccgcgtgcct ttttcagaag
      721 cgatctcttg tgcaatgatc tgcgcatacg tcaaaataat aatgaacaag acaatcgttg
      781 ctacataact tacgacgtat tggactgtcg agttatcttc tccgactgtc atttttcctt
      841 ttgaatcaaa attgactttt tgtcgagtca aacttgcttg ttggctcaag ctagctactt
      901 cttctggtga aatgcctaat tgactcgctc gaagcatcga ttgcaagcca gtcagttgtt
      961 gttgtatcag tagttgtgta ctttgtccta atgaattttc gctataaagc atcccattta
     1021 tcttatcatt ctcagttgag actactaaat acgcatcgat tttttcttcc gaaagctctt
     1081 tttgtgcttt ttcttcagaa tcaactgctt tgaactcaaa atcatctgtt ttgacttggc
     1141 tcatttgctg actgatttgc tcattatcag ccaatacgcc gattttattg acatcactat
     1201 tttgttgagc aaagtttcct gctacataaa tgatacccag catgataaaa gggactaata
     1261 tcatgatcaa gaaagaaatc gacttaacat tttttaaata gacatctttg gtaataatcc
     1321 aaaacttacg catcgatttc acctgctttc aatttgaaaa tctcttccaa agttggcggt
     1381 tgctggttaa acataggaat gtagccgaac tgagtcgcac gagtaaagat ttctttccca
     1441 gcttcaggat cttctaaggt aatctctaac gtattgtctt catgaaggat cacttttttc
     1501 acgccttcga tttcttctac ttcctgttgg cttaaaccag attccaagaa taacttcgta
     1561 cggccaaatg attcacgaat ctcatgtact tttccattca aaaccatttt tccgtttcgc
     1621 aacatgatca aatgatcgca gattttttct acattatcca tattgtggct agaaaaaatg
     1681 acacatgaac catgtttttt caattccaat attccatctt ttagcaattc agcatttacc
     1741 ggatctaacc cgctgaatgg ctcatccaaa atgatcaatt taggttcatg gattaacgtg
     1801 gcgatcagct ggactttttg ctgatttcct ttagacaaag attttacttt atccgtcttt
     1861 ttccctttta cctgaaattt ttccatccaa aagtcgattt ttggttcaat ctcttttctt
     1921 gatttccctc taagttctgc aaaataaagc agttggtctt gaatcgttac tttcggataa
     1981 agtccccgtt cctccggaag ataaccgatg acatcataat ctttctcccc aagatgatgg
     2041 ccattccata acacttctcc ttggtcttgt gtcaaaaaat ccaagatcaa acgaaaagtc
     2101 gttgtcttcc cagccccgtt ttggccaatt agccccatga tttttccatc ggggatttgg
     2161 aaagagacat gatcgacagc tgtgtagttc ccaaaagttt taacaagatc cttgacttct
     2221 aacatttctt cactccttct tcaagaaatc acatatatcc ttagacacca gacgtaagcg
     2281 atttattatt taccaacaaa acgtaaacgc tctttttgtt gtacataaaa tgataggaca
     2341 ctttactttc tttgtaaagt gccctttcct cattcctgat gtaatttatc aaatacgttt
     2401 tgtgcgacat ttttcggcag tccgatttct tgtaattctt ctgtcgtggc tgctgtgata
     2461 tttttcaacg atttgaactc tttaagcagc attttttttc gttttggtcc taacccttca
     2521 atttcatcta atcgggaagc aaaactgttt ttactgcgta gctgccgatg gaaagtgatt
     2581 gcgaatcgat ggacttcatc ttggattcgc tggagtaaga aaaactctgg tgaatttcgc
     2641 tcaagcggta ctacagccaa atctgtaccg aatagcaact catttgtttt atgtttatcg
     2701 ttttttacca tgccagcaat aggaatatcg attcctaatt gattttctaa gacatctttt
     2761 gccacatcta cttgtccttt tcctccatcg atcaagatca aatcaggtaa aggcaaccct
     2821 tcgcgaatca ctcgcgaata tctgcgatag atgacttctc tcattgatgc ataatcatcc
     2881 ggtccgttga ctgtttttat tttatatttc cgatattctt ttcttgccgg ttttccatca
     2941 atgaatacga ccatcgcagc cacaggattc gtccccatga tattagagtt atcaaaagac
     3001 tcgatacgta ttggggtcgg gatattcatc gcgtttccta aacgttcaac tgctccgatc
     3061 gtccgttcct gttttctggc aatcagatca aaccgttcat tcaaagcaac tgaggcattc
     3121 ttactcgcca attcaactaa ttttttcttt tctcctcgtt ttggttgcag cactttcgta
     3181 gctaacaaag cttctacact gggcttgtcg atcgtatcag gaatcaagac ttctttagga
     3241 atgaaatgct cattttcctg ataaaactga ccgaggtaag tcaagaaatc ttcttctgct
     3301 tcgttataga aaggaaacat tgaaacatct cgttcaatta gctttccttg tcttacgaaa
     3361 aacacttgga cacacatcca tcctttatca atcgcataac caaatacatc tcgatccagt
     3421 aaatcagtat ttgtcatttt ttggcgagtc atgattgtct caatggcacg gatctggtct
     3481 cggtattctg cagctttttc aaactccatg ttttcagcag ctttttccat tttctgatgg
     3541 atctctgact cgattttttc gtgtcctccg tttaaaaagt ttttcacttc atcaacgatt
     3601 ttcgtataga ctttcggatc tacgtcgaaa taataaggac acaggcattg tcccaaatga
     3661 tagtaaagac aaggctcctt cgtctgacta ggtttgcatt ttctcagagg aaataatcga
     3721 tcgaggatct tctttgtctc attcgccgca ccgacatcgg gatatggtcc aaaatacagt
     3781 gctttgtctt ttagtacctt acgagtaatc accaaacggg gatatttttc attcgttatc
     3841 ttaaggaaag gataggtttt atcatccttt aacataatgt tgtatttagg atcatttttc
     3901 tttatcagat tgatctctaa gagcaatgct tcgatattcg attctgtaac gatgtattca
     3961 aagtcttcga tttcactaac aagccgttcc gttttcgtat tatggctacc agtaaaatat
     4021 gagcggacac gattttttaa tattttggct ttgccgacat aaatgatcgt tccattttta
     4081 tctttcatca agtaacagcc gggctgatca ggaagtaaag ctaatttgtt tttgattcgt
     4141 tcgttcatct ggtactcccc ttcatattca attaaagtta ttatagcatg tctgtcaaaa
     4201 aaacaatcca gcctgacaac gtaattatac tcaaaaaata ttaatttgaa taaccttaac
     4261 tcaaccgcaa aaaaaccgag gacggaactg gattcagtcc caccctcagc ttatttttgc
     4321 tgtaagtttt tataaatatt gatcgatcat ttgacgtagt tgttcttttg aatgaacacc
     4381 aacagctttt tctactactt gaccatcttt tttcaacatt aatgttggaa tgctcatgat
     4441 accgaattgt tgaggagttt ctggattttc atccacatcc attttagcaa ttctgaattc
     4501 ttcttcatca tattcttgtt ccaactgatc caaaatcggt gcttgcatac gacatggacc
     4561 acaccaagtt gcccaaaaat caatcaatac aagtccttta tctgtttctt cattaaatgt
     4621 cttatctgta attacttgtg acatatcaaa gacctccatt tttaaaatct atgactagta
     4681 tagcatttct atacttggat agcgaaccat ctgcttacct ttagtaagct ttacttaaat
     4741 ttaacaattg tcgcaccatt tccgccttgg tttgccggtg caaactcaaa gcttttgacg
     4801 ctccgatggt ttttaagata atcagtaatt cccttgcgaa gtgcgcctgt tccttttccg
     4861 tggacaatag tcacttgagg ataaccagct agaatggcag aatccaaata ttgatctacc
     4921 tcacttagcg cttcttcgta tcgttttcct cgaaggtcca attgattcgg cacgtgacta
     4981 ctagattcag atcgtacagc atggataact cgttgtgtcg gctctttttg aggagcaaca
     5041 ggcgtcagct cgtcttcgga cacattcatt tttaatatac cgatttctac ttgccactga
     5101 ttcccgtttt tacgtaaaag cgtccctctt tggccgtaag tcgtaaccaa cacttcgtct
     5161 cccggtttca acgttttttg ttctttcgcc ttcttcaata ctttattttt cttcaaagtt
     5221 tcttcttgat gtagatttgc taattgggtc tttgcatcga tcaactgatg ctctttcaca
     5281 tttccttgcc caatctgctg ttgcatttta cgaatatcag caatgatttt ttctgctttt
     5341 tcttccgcct ctgacacgac ttcatttgct tttttctttg ctttttccat ttctttttca
     5401 cgttcttcaa agaaatagct gtaagcttct ttcaaatcat cgtgaagttc ttgtgcctca
     5461 gaaacaaaat gacgcatttc cagatattcg gtctctgcca ttttccggcg attctctaaa
     5521 tctgtgatca tttcgtttaa atcttggctc tcatcattca tcaactgctt cgcttcatca
     5581 ataacttctg tatcaagacc taatcggcta gagatttcaa acgcattgct tcgtccagga
     5641 acgccaatca gcaaacgata cgttggactt aatgtatcca catcaaactc catgctggca
     5701 ttaatcgtat ttgcccgatt atatccataa actttcaatt caggataatg cgttgttgcc
     5761 atcacataag cagacttttt accaagatca tctaagattg cgattgctag agcagcacct
     5821 tcttgcggat ctgttcctgc accgagctcg tcaaataata ccagactttt ttcattgact
     5881 tttgataaaa tatctacagt gttcgtcata tgagaagaaa aggtcgatag actttgctca
     5941 atcgactgtt cgtctcctat atccgcaaat acttcctcaa aaataccgat ttgactttct
     6001 tcacctgcag gaatcggtaa tcctgcttgc cccatcagtt gtaacagacc taaagtcttc
     6061 aacgtgatcg ttttcccacc tgtgttaggt cctgtaatga cgatggcttg atagtctttt
     6121 ccgattgtga tgtcattaga aactactttt tcctgatcga tcagtggatg tcttgcttgt
     6181 ttcaagacga catgattgtc ttcactgatt cccggcacga tggcttttac ttctttacca
     6241 aatcgtgctt ttgcattcat cagatccatt ttaccaatca cataagtatt gtggatgatt
     6301 tcttgacgat ggggaacaag ttcagcagaa agttcactta aaatacgttg aatctcattt
     6361 cgttcagcga tttggtattg acgcaaacga ttattcaaat ccaccacttg ttttggttca
     6421 ataaataaag tctgacctga agcactttga tcgtggacta caccgccaaa tacaccgcgg
     6481 tattcttgtt tgactgggat aacgtagcgg tcatttcgca tggtgatgat cgcatcactt
     6541 aaatactttg catttttccc acggacgatc ccatctaact gttcacgaac agcttgttca
     6601 cttcgccgaa tgttctgtct tatgctcttt aactcaggag aagcgtcatc agtcacgcgt
     6661 ccgtcttcat cgattgcttc ctttaaacgg cggctcaaaa cagggatcgt gactaactga
     6721 tctgcccaca tatacaaacg tgcaaattcg atttcactgt cagaaagatc atcaatgaaa
     6781 cgattcaatt cgcttgtcgt agacaatact ctggaaactt gtgcaagctc cactccattc
     6841 aagtctgctc cgatctcaat ccgtttcata tggggacgga tattttcaat tttaggtata
     6901 gggatcccgc ctcgcagacg ttgcactttc aatccatcct cagtttcttg aagccagttt
     6961 gtgatcgttt gtttctcact cactggtacc aattctgcca gttcttcttt tccttgtgca
     7021 gtcactacaa attgtctgac catctgtttg actttttcaa attccaatgt ttcaaggatt
     7081 cggttattca ttttatcacc ttatctttca ctatgaaaaa agagctgtgc cggacatacg
     7141 ctacgcagcc ttcttaaata aactactctg tacaaaaaac gaaatatttt ccagcactat
     7201 tttccttaat tcaaaatatt cgttacccaa agctcataca tcttgttggt aagaaatggc
     7261 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacatct
     7321 aatggaacaa aggcaagtaa ctgcaaaatc aaaaatatag taacgtaagc catgattagt
     7381 gacaggatgc ctcctgcaat ccagtctacc tgtctcaaga tcggcacata cgtcaatcca
     7441 tggacaaaaa cccccacaaa tcgggtaagc agccagccaa ttagcaaaat aatcagaaaa
     7501 gcagttcctg cataaaaagc ttcatctagg tgaaaggcaa atacctgatc aaaaaatgct
     7561 aactttgatg ttggggtgac agctgggtat ggaatgtata attccaaatg acttgctaat
     7621 ggtttgtaaa aatgctgggc agcaataaat gacaatacat atccaatcgc gaatatgatt
     7681 tgcaaagcaa acccgcgacg tgctccagaa aaaaacgcaa ttagcaataa aaataaaatc
     7741 agcaaactca gcatgctacg ttcctttctc ttctaacttt tttaaatttt tcttattttg
     7801 ataaaaggat cttaattaga agatttttgt ttaatttgtc cttttcgttt ctcgtttaaa
     7861 atctgttgtg cttccacatg atttttaatg gtatgatctg cttgaccatt ttctttcaat
     7921 acttcccgag cttcgttctc gattgcttca atgcgtttca cacgattttc aagctctgta
     7981 aatttgatca tttttttctt taattctgct gtttcttctt ccagctccaa tattctttct
     8041 tgtttcttca attgatccga aagcgcattg acagccatca agattgctgc ttgttcatta
     8101 tccatttgtg gtgataattg tttcaattct gctaactgtt cattgatcag cttcgttacg
     8161 atatccatat gatgcttggt ttcccgtccg ataatagtat atgtttggtt ggcaatcacc
     8221 gctttgtagc gtgttttttc gtgcgccata cgaatcattt cctcctattt gatgaaagtt
     8281 ttatttagtg taccatgaag ttggttcttt caccgttctc cacaaatatt tttcacatac
     8341 ttaactatta tatgataaac tttaaaaaaa tgctatgtct cgcaatctct tagaaaacga
     8401 aaagtcgtga gttgcggtat agttttatgt ataaaaacga ggagaatttt atgagcaacg
     8461 tcattatcaa agcaacaaac gaccaactta gtcaaatgaa agcatactat cgctcttatt
     8521 tgttaaataa acagattcct tatactagct ttgctgcaaa aaaagacggg acgaccatta
     8581 ccgcttatac atccggaaaa gtgatgtttc aaggtacgaa tgcagcaata gaagctgata
     8641 aatgggggga ggcacaggat aaacaagcca agccagcaaa aacagcctct tctttgccaa
     8701 aaaatatcag tcaattatcc gttttaggta gtgatgaggt cggaaatggc agctatttcg
     8761 gtccgctaac agtatgtgct gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac
     8821 ttggtgttag agattcaaaa gagttaaaag atagccaaat catccagctt gctgacatct
     8881 taaaaaaaac catcccctat aaactgcttg tcttatctcc agaaaaatac aatgagatcc
     8941 aaccaaaata caatgccgtt cggatgaaag tcgctttgca caatcaagcg atccatttgc
     9001 tgctaaaaaa aatcgagcca gaaaaaccag aagcgatttt gatcgatcag tttacgccgg
     9061 aagcaaattt taaaaaatat gcgcgtttag aaaaaaatca attacaagaa aaactgtact
     9121 tcgttactaa aggagaacaa tatcatttag ctgttgctgc tgcttctatc atcagtcgtg
     9181 ccagcttttt ggaagagtta gacaaagcat cagctgaagc tggaatcacc cttccgtcag
     9241 gagctggaac gaaatctgac caagtagcgg caaaactatt ggagaaaggc ggcatgccta
     9301 tgcttgaaaa atatgccaaa cttcattttg ccaatacaga aaaagctttg aagctactaa
     9361 aaaaataatg gaactgtgac aaaagtcatg tcacagtccc ttgttccgaa taaacggtgt
     9421 tcaatcatct gatcctcggt attagctcaa caaacggcaa aatatgagga actatttttc
     9481 cgtttgtttc tctaatactc agaacaaaac gttgatttca caaccttttt cttaaacggt
     9541 gttctcatgc caactcctcg gtcacaagtc acaatagtca tcaaacatga gaagctgttt
     9601 tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc ttccttaaac
     9661 ggtgtttaaa agcttgcatc tgcgataata agcccggcca aacgaaaaat atgaggagct
     9721 atttttgttt ggctgggctt atttattact gaaaataagt ggtcaatgct tgtgtcaaac
     9781 ttgcagcaat ctgctgctga taactgtctg ttactaattc ctttaaatca ctatcattat
     9841 tcatataacc catctcgatt aaaatagaag gttgaagatt ttctcgaaga acttgatagt
     9901 tcccagtgga gattcctctt gaatccagag gcagatcagt gaaggagtca gcaattgtct
     9961 gtgctaatgt ttcctcttgt ccatagtaat aatatgtcgt gattccagtt gcttcattcg
    10021 catattccgt agagtctgcg tgtaaactga taaagacatc tgcctttttt tcttcactgt
    10081 agtcgcatat atctccaagc tgtactaatt catctgtagt atgggtcaaa taaaccgttg
    10141 ctccagcatc ctctagcgcc ttttttactt ttatggctgt acttaatgtt atatcttttt
    10201 cttctgtttg ttcatcattg cttaatgccc cagtatcatt tccgccatgc ccaggatcta
    10261 atacaatcac tgcatcagat agctcttccg gttttgtttg tatattcttt gtgactttgg
    10321 ttccatcttc gagggctaaa tatccagtat agccatcatt tgtcttgatc tgataagcat
    10381 tttttgtttc agaaagaaac tccgccagct ctccgccgtt tatagttgca agtggggtag
    10441 ttagcgagtc atctgtatat agtgtacggt cattttttcc gattttgatt aatgtctctt
    10501 gttctgtatc atcaaatgat tcctttgtct ttgtaaccgt actgttcgta ctgctgaccg
    10561 tttgtccaac tatctttttt tgaccaaaaa catctatttg aaaacagata ataatgataa
    10621 tgattgcaaa tacaaaagct ccaattattt ttttcattct tgactctcac ctcgtagatc
    10681 cagactaagg gccaaaactt aaaatcagct taagtaagca aaaaaggggc tgtaaaagat
    10741 ataaccgtag aaaataacat tactgctaaa atgatttttt tcatttttgg catatcctga
    10801 aatctccttt tgactattcg tggtgattca tctacccaat aaatagtttc cttcattcta
    10861 caaacaatta atttcttcgt tattaaatta aaacacttcc tg
//
LOCUS       DAYYKC010000095        10666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000095 DAYYKC010000000
VERSION     DAYYKC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.096"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1031)
                     /locus_tag="ACHBBW_002398"
     CDS             complement(183..1031)
                     /locus_tag="ACHBBW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9154054.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="ACHBBW_002399"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="ACHBBW_002399"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9154055.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="ACHBBW_002400"
     CDS             2099..3100
                     /locus_tag="ACHBBW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9154056.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="ACHBBW_002401"
     CDS             3097..3381
                     /locus_tag="ACHBBW_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9154057.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="ACHBBW_002402"
     CDS             3414..4211
                     /locus_tag="ACHBBW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9154058.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="ACHBBW_002403"
     CDS             4225..5058
                     /locus_tag="ACHBBW_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9154059.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="ACHBBW_002404"
     CDS             5076..5933
                     /locus_tag="ACHBBW_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9154060.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="ACHBBW_002405"
     CDS             5955..6464
                     /locus_tag="ACHBBW_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9154061.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="ACHBBW_002406"
     CDS             6501..6902
                     /locus_tag="ACHBBW_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154062.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="ACHBBW_002407"
     CDS             7014..7367
                     /locus_tag="ACHBBW_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154063.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="ACHBBW_002408"
     CDS             7401..7844
                     /locus_tag="ACHBBW_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9154064.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002409"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002409"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9154065.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="ACHBBW_002410"
     CDS             8939..9652
                     /locus_tag="ACHBBW_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9154066.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9859..10008)
                     /locus_tag="ACHBBW_002411"
     CDS             complement(9859..10008)
                     /locus_tag="ACHBBW_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154067.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10404..10604
                     /locus_tag="ACHBBW_002412"
     CDS             10404..10604
                     /locus_tag="ACHBBW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9154068.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3559 a         1691 c         2314 g         3102 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttaattgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10261 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10321 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10381 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10441 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10501 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10561 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10621 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DAYYKC010000096        10663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000096 DAYYKC010000000
VERSION     DAYYKC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.097"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHBBW_002413"
     CDS             33..656
                     /locus_tag="ACHBBW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154069.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBBW_002414"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBBW_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9154070.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHBBW_002415"
     CDS             2152..3033
                     /locus_tag="ACHBBW_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154071.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHBBW_002416"
     CDS             3036..3806
                     /locus_tag="ACHBBW_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154072.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBBW_002417"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBBW_002417"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9154073.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHBBW_002418"
     CDS             5117..6280
                     /locus_tag="ACHBBW_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9154074.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHBBW_002419"
     CDS             6397..6807
                     /locus_tag="ACHBBW_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9154075.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHBBW_002420"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHBBW_002420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9154076.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHBBW_002421"
     CDS             9380..9664
                     /locus_tag="ACHBBW_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154077.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHBBW_002422"
     CDS             9824..10060
                     /locus_tag="ACHBBW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9154078.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHBBW_002423"
     CDS             10057..10455
                     /locus_tag="ACHBBW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9154079.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3725 a         1773 c         2253 g         2912 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat ttt
//
LOCUS       DAYYKC010000097        10318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000097 DAYYKC010000000
VERSION     DAYYKC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.098"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..251
                     /locus_tag="ACHBBW_002424"
     CDS             <1..251
                     /locus_tag="ACHBBW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154080.1"
                     /translation="RLETAYQTRFDMKASLVFLNDAYQNSIELIKAVDENPTNECEEF
                     LNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            270..1478
                     /locus_tag="ACHBBW_002425"
     CDS             270..1478
                     /locus_tag="ACHBBW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9154081.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1626..1991)
                     /locus_tag="ACHBBW_002426"
     CDS             complement(1626..1991)
                     /locus_tag="ACHBBW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9154082.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2138..2911)
                     /locus_tag="ACHBBW_002427"
     CDS             complement(2138..2911)
                     /locus_tag="ACHBBW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9154083.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3085..3945)
                     /locus_tag="ACHBBW_002428"
     CDS             complement(3085..3945)
                     /locus_tag="ACHBBW_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9154084.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3945..5363)
                     /locus_tag="ACHBBW_002429"
     CDS             complement(3945..5363)
                     /locus_tag="ACHBBW_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9154085.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5420..5869)
                     /locus_tag="ACHBBW_002430"
     CDS             complement(5420..5869)
                     /locus_tag="ACHBBW_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9154086.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6314..6577)
                     /locus_tag="ACHBBW_002431"
     CDS             complement(6314..6577)
                     /locus_tag="ACHBBW_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9154087.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6676..7971)
                     /gene="rho"
                     /locus_tag="ACHBBW_002432"
     CDS             complement(6676..7971)
                     /gene="rho"
                     /locus_tag="ACHBBW_002432"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9154088.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7968..9239)
                     /locus_tag="ACHBBW_002433"
     CDS             complement(7968..9239)
                     /locus_tag="ACHBBW_002433"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9154089.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9404..10273)
                     /locus_tag="ACHBBW_002434"
     CDS             complement(9404..10273)
                     /locus_tag="ACHBBW_002434"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9154090.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2859 a         2197 c         1823 g         3439 t
ORIGIN      
        1 atcgattaga aacagcatat caaacaagat ttgatatgaa agcttcttta gtctttttaa
       61 atgatgcgta tcaaaactcg atcgaattga tcaaagcagt ggatgagaat ccaacaaacg
      121 aatgcgaaga atttttaaat ctgtttatgt ctacaagaga tttatttatt cgccaacttg
      181 ttgatcgcta tccatccaat tatcacgatg tagaagttca aatacaaaaa ttaaaagcat
      241 attcagctta aggggaatgt ataacataca tggaaaagaa attatttaat aaatatttta
      301 ttggaatcac attgatcaat tttattgtct acctgattta ttatctgttg atggtcatca
      361 tggcagtcat tgcgcaagac accttacacg ccagcttgag ccaagcaggt tttgcttctg
      421 ggatctatat catcggaaca ttgctggcac gcttattcat ggggaaacag ttagaactat
      481 ttggtcggaa gtttacctta agaggcggcg cgatcttcta tctcctttca acattagcat
      541 atctttatac gccaactatc ggggcattgt atttagttcg attcttgaat ggattcggct
      601 acggtacagt ttctacagct acaaatgcta tcgttactgc atatatccct gcatctaaaa
      661 aaggggaagg catcaattat tatggattaa gtactagttt agctgccgct gttgggccat
      721 ttttaggaat gattttgctg aatatgactg attttcgttt cattatctgg ttttcaattg
      781 tactggtctt ctttgttacg attgcctgcc ttgtctttcc tgtaaaaaat attccattat
      841 ctgaagaaca aaaaatatct cttcgctcat ggaatatcaa tagttttatt gaaaagaaag
      901 cacttttcat tacaggaatt gcctttttga tgggtctttc ttattcaagt gttttatcat
      961 ttttatcttc ttatacaaaa gtaatccact tagttgctgc tgggtcattt ttcttcgtcg
     1021 tatatgcttt ggtcatcaca tttacacgtc cattgactgg aaggattttc gatgtcaaag
     1081 gagaacaata cgtgatgtat cctagctatt tattcttaac agcaggatta tttttactga
     1141 gtgtaacgac gaatagtgtg acattgctta tctctggagc attagtcggc ctaggttatg
     1201 gtacgtttat gtcaaatgga caggctgttt gtttaaaaat cgtccgcgaa catcgaatca
     1261 gtattgcttt atctacctac tttattggtt tagacctagg cttaggcgtt ggtccttatt
     1321 tgatggggat gctaaaatct cttacttcat ttagaggatt gtatgtgatt gccggagtca
     1381 ttccattgat atgtacagca ctctatcttg ttaatggccg aaaacatagt catgaagcag
     1441 agggcgaatt gattgaatca ttgaaaaatg aaatttgatc ttgatgaata gataaaaaaa
     1501 accgttcaga tctgaaaata atctgagcgg ttttttgagt aaaaatagtt tatgattatt
     1561 cttaattaaa catctattta tttttaaagc tttcatttat cgataagaat agtttaactg
     1621 ttgatttatt ccattttccg ttcatctggt acgatgcctt gacttagcaa ccacttgtgg
     1681 aagaaccatt ctgccacacc gatcaataga gcaggaatca atactttcat ccaaatatcg
     1741 actgtatcat tcataaaata tagatagagc cacaagataa ctgcagctga accagcatct
     1801 gcgatcgtcg ctgcgatgtt ccctacttta cgtaagatca acagatcccc tagcacatag
     1861 atcatgacta caataattac cccaacaatc gttgaatcca taaagctaac accgtataaa
     1921 cctgtcagca cgacccacag aatcgcccag atcatgattg ccttcactac caacgccttc
     1981 acatgtttca ttttctgcac tcctttctcc tggataaaaa agtaagatta tctaacaaga
     2041 taaccttttc attattatac catcggctga gagaagcgag caaagataag gtcctatcta
     2101 aaaatgagga agccacttag aacattcacg taataattta tccgtacaga cattcgatat
     2161 ggtgttcctt tactttcgca tactgttctt ttgtcagttc tttacttgtc accagataat
     2221 cgatttcatg aaaagcaatg gcttgatgca tgaatttatg tgcaattttt tgctgtgtaa
     2281 ctaaaacggc aagccggtct gattgttttc taaaagcttt tttgaccatt gcctctcctt
     2341 catgagtttc tgttactcct tggctgaaat ctagcccgcg acaactcatt atcgctaaat
     2401 ccgcataata tgacaatgca tcgttatagg cttttgaacc gttgattgtt gctcgtttcg
     2461 gaacaattgc ccctcccaat acagatactt ttgccgttgt atattcactc aacatagcag
     2521 ctgtgacgat gccattcgta atgatctgca aaccatcaaa tcttgctagg tttctgacta
     2581 agtgtaaaga ggttgtactg gaatccaaga ataagaccat atcatctcga atcaaagaag
     2641 aagctgtatc tgcaatctta cgtttttctg tttcttcttc taatttggtg atcttcatca
     2701 ggtaaggcac gtcaattttt cgattgtcaa taagcatggc gccaccgcga actctacgga
     2761 tcaatccact tttttccaga aaaacaaggt cacgtttaat cgtggcttcg ctataattta
     2821 ctaactcgat caaatcggtg actcttgcaa attggttgtt ttttagataa ttcacaatct
     2881 cctgctgtct attctccact tcattcaaca taaaatcacc ccatttactt tatatagtaa
     2941 caggaaaaaa gaggttgtga cagaagtgat tggcttcgag aaataagaag aaatttttcg
     3001 aaaaaactac ttctgaacat cgtgtgtggt taagagggta tgacaagact attgtcatac
     3061 cctcttgacc acacattttt atttttagta taaactagcc ttgcctactg tgttcaaaat
     3121 ttccatttta tgtttgacca cttcttgtac ctttttatta gctgctggat aaattgcatt
     3181 cggctcgtac atcgatggat tttcactcaa tatttctctc aaagcattga aaaagacgct
     3241 tttaagatct gagctcaaat tcactttggc aactccgtat tttactgctt cgctgacttc
     3301 tgcgtccgga ttacctgaac cgccatgtaa aacaaatgga agatcgattt gttggttcaa
     3361 ttctttcagt aagtcgatat ttaattttgg agttctgttt ttcggataca agccatgcgc
     3421 tgttccaatt gctacagcaa gtgtatcgat tcctgtttct ttcacaaaaa tcttcgcttg
     3481 atcaggatct gtataaatga ttgtttcagc acctccttca gcagaaccat tattcccaat
     3541 cgttccaagc tcagcctcca ctgagacatt taccgcatga gctaacgcaa ccactttttt
     3601 cgtaatctgt acattgtcgt cgaatgattt caaagacgca tcgatcatca cggaagaata
     3661 gccgtttcga atcgcccgta agacatcttc tatcgtaccg ccatgatcca agtggataac
     3721 gatcggcagt ttacttgtat aagcataggc tcttactgcc gcaacaaact catcacttaa
     3781 ataggcgatt tcatcaggat gaatttctaa gattactggt gcttctaatc tctctgcttc
     3841 ttcgatgatt gctttcatca tatcatagct gcaaatattg aaggctggta ctgcaaattg
     3901 attctcttta gccactttca ataactcttt cattgtatat aacattacgc ttccacctca
     3961 tttgttagat tttcattttt cgtccacttg tcttgttttt tctctttcac tgtttttttc
     4021 aatagtccaa gcataaaggc tgtagtgact gtacctgatg caattgccaa gacatacata
     4081 ggtaaattgt tcataacatt tggaattagc atcacccaaa tccctccatg aggtgccatt
     4141 gaagtaactc ctactgccat ggaaatccct gcagcgactg ctgaacctgt catcaaacta
     4201 ggtatcacgc ggatcgggtc agataccgca aacggtatag caccttctgt aatatacgaa
     4261 gcgcctaaaa tccagcatga ttgacctgaa gtccgttctt ctttggtaaa tttatgtttg
     4321 aataataaag tggctaaagc aatacctagg ggaggtgtca ttcctgcagc catacaagca
     4381 gcaatcggtg catttactcc agctgacatc aaaccgatcg agaaagtcga agtcgattta
     4441 ttgatcggcc cacccatatc agctgccatc atgatcccga taagtaatcc gaacaatagc
     4501 ccggatgtat ttcccaagct attcagccaa tttgtcaaag catccaacac ccatgtgatc
     4561 ggttgttcaa tcacaaatac catcaaaagc ccaataatta agatcgataa aaaggggacg
     4621 acaatcagct gataaatagc agaaaattct ttttttacgc taattttatt tgccagaaaa
     4681 tcacagatat accctgctag cacgccacca atcattccgc caagaaaacc tgaaccacct
     4741 gtagaggcca gtaatccggc aacaattcct ggggcaaaac ctgcttttcc agcaatcgat
     4801 gtagcaatcc ccgcgcctaa cgcaggaacc atcatggcaa acgctgtatc tcctccgatc
     4861 cttgaaaatg cagctgctaa cacattatag tctgctgagt tgggatcagc cgctgtaatc
     4921 ccaaaggcaa aactcagtgc gatgatgatt ccgcctgcga tcacaaatgg aagcatgtaa
     4981 ttcacaccat tcatgaaatg attatagatt gtcggctttt tctcatttgc agctggttct
     5041 ttttcattag acacaacgaa tacgccaata tctctcaatg cttcgtctat tactacttgc
     5101 ggttctttga ttgctcggct tgtagagaca cgctttactt ttttcccggc aaatcggtcc
     5161 aattcgattt ctttatctac tgcaagaatg attgcatctg cctctttgat ttcttgttca
     5221 gataactgat tttctgtttt tacaccttgt gtttcgaatt ttgcttgatg gtttagattt
     5281 cttgctgtta cttctaattt ttctgccgac atataagtat gagcaattcc agtcgggcaa
     5341 gcagttacac caacgatttt catgattatt ttcctccttt ttcttcacta tcgatactcg
     5401 gacaaagact ctactatttt cattcttgac taatctgtct gatgatatct gctggcgtag
     5461 tggcttcttt tattcccttt ctaaaatctg cattcatcaa tcgtctggag agtgtgctga
     5521 gaatctctag atgcatatct gctgattttt caggaactac gatggcaaaa atatattcaa
     5581 ttggcatttc atctatcccg ctatagtccg ttaatttggc tgtatgaaaa aaacacagtg
     5641 ctggttttgt cacatatttg ctttttgcat ggggaatccc aaatccatct tctattcctg
     5701 tggagatctc ctcctctcgt ttgaatagtg ccttaacgac tttttgaaca tttcttacct
     5761 tatttgtttt tttcaacaca tcccctattc gctcaaataa tgatcctttt gaatctatcg
     5821 atggatcgaa aacaatcagt tcttctgcga tcaattgatc aaacctcata aaaaacctct
     5881 cccccacaaa aaatgttaac gttttcttgg aactgaggtc agtatagcat cgctagctgt
     5941 accgttcaat attatgaatg aacagaggaa aaagacccat catccgcgtg tataagtcat
     6001 taaaatgaac gatctagctc acaaacaatc gcaagtctat aacaaaacaa aaagccttcc
     6061 ttgaaaggaa agcttagctt ctaattttag gtaaatggtt ttttacttcg aaataacccc
     6121 tcttcatttc gttaatataa ggtgttttta tcaagtggat aattaaaact atatactagt
     6181 ttttcattgt tttgtgcatt atgatcctac gactttttca gcatcaaacg aagttttaaa
     6241 ttggcaaaaa acataaaaaa acagactatc cctatcagat agtctgctaa acgttgagtt
     6301 atcagcgttt ttattattcc gcagcgtttg cgtctttgag accgtatttt ttgttgaaac
     6361 ggtccacacg tccgtctgct tgtgtgaatt tttgacgtcc agtgtagaat ggatgtgagt
     6421 cagaagttac ttcgacacgg atgactgggt atgtattacc gtcttcccat tcaactgttt
     6481 cttgtgaagt ttttgttgaa cctgacaaga atttgaaacc agttgttgaa tccatgaata
     6541 ctactgggtg gtaatctgga tggatgtttt ctttcatgat tttttcgctc ctttgccctg
     6601 ctccatttgc tgaaccagag ttgatttgtc gatttacaac attaacatct taacatgttt
     6661 cagtcaaggt tgcaattatc ttttcagatt tctggtcgga ttttttttac caaaggagac
     6721 atcttgaaat tctttgaaaa attgctgatt gttttttgtt ttacgcaaaa agcggatcag
     6781 ttgttccgta tattctaatg aatctccagt catatttttt cgaagtttcc aaatttcttc
     6841 taattcttca cttggcatca ataattcttc ttttcgggta cctgattttt tgatatcgat
     6901 ggctgggaag atccgacgtt cagataattc acgtgaaagt tgtaattcta gatttccagt
     6961 tcctttgaat tcttcataga tcacgtcatc catacggctt cctgtatcga ctaaagcggt
     7021 cgctaaaatc gtcaaactgc caccttcttc gatgtttctt gctgccccga aaaatttctt
     7081 aggtttatac aatgcggctg ggtcaatccc gccactcaat gtacgtccgc ttggtggaac
     7141 gactaagtta taggcccgag caagtctagt aatactatcc atcaagataa cgacatctcg
     7201 tttatcttcg actaaacgca ttgcgcgttc caatactaat tcagaaactc gagtatggtt
     7261 ttgcggctgc agatcaaatg tagaagatac cacgtcgcct ttcacgcttc gttctagatc
     7321 cgttacttct tccggtcgtt catcaatcag tagaacgatt aattctactt ccggatagtt
     7381 ttcggtgata ccatttgcga tttcttttaa tacagaagtt ttccctgctt ttggcggtgc
     7441 gacgatcaat ccacgctgac caaaaccgat tggagaaaag atatcgatca ttcgggtaga
     7501 tagtctttgc ggtgttgttt ccagttgaag ctgtctttgc ggataaagag gtgttaaggc
     7561 tggaaaatgc ggacgttctt ttgcttcttc cggatctttt ccattaacgc tttccacgtg
     7621 catcaaacca taataacgtt ccgattcttt aggtggacgg gcttttcctg ccactttgtc
     7681 cccatttctc aaactgaatc gtctgatttg tgaggcagaa atatagatat cttctgcact
     7741 tggtccataa ttgattggcc gtaaaaaacc gtaaccatct tgaccaacga tatccaatat
     7801 tccttccata taataaaatc cttgtttctc tgcttgtgca cgaatcacag ctagagataa
     7861 ttccttttta ttcatcttac tgtaataagg aattttaaat tctttggcat aactataaac
     7921 ttcttttaac gttttgttct ctagttctgc cattgtcaaa taatcgctca ttcagctccc
     7981 acttcctcta tcatttcaat tgatgcccct aaggctgtta gtttctcgat aatatgatca
     8041 taaccgcgta agatataatc tacattgtaa atcgtcgttg tcccttctgc caatagtcca
     8101 gcaatcacta aacaagcacc tgcccgaaga tcagaagcca cgacttctgt gccgtgaagt
     8161 ttatttggac cattcacgat gatcatattt ccttccacgg taatatcagc gcccattcgt
     8221 gctagttccg gcacatggtt cacacgtttc gcatagatcg tatcgatgat ttctgaagtg
     8281 cctgtagtca tcaatagtaa cgcagtaagt ggctgctgca gatctgtggc aaatcctgga
     8341 taaggatagg tcatgatgtt tgcgggtttt aattctttcg aggggtaaac ttcgatttca
     8401 tcttcgctga ttttcatatg gacaccaatt tcctgcaatt ttgctataaa gctttccaaa
     8461 tgttcaaaga tcacattttt cactttgata ccatttccta ctgcagctgc taatgctaga
     8521 taggttcctg cttcgatgcg gtcaggaatc atgaaatgcc gacagccatg taatgtttcg
     8581 actccttcga tacggatcac atctgtaccc gctcctcgaa cttttgcccc catattattc
     8641 agtaaagttg ccacatctat gatttccggt tctcgtgcag cgttttcaat tactgtctgt
     8701 ccttttgctt ttacagcagc taacatcaca ttgatcgttg ctccgactga aaccacgtcc
     8761 atgaaaatcc gattgccacg caatccttta ttttctgtcc gtaaatacat ggcaccatgt
     8821 tcatttgtca cttcagcacc tagagcttca aatcctttga cgtgtaggtc aatcggtcta
     8881 gggcctaaat aacagccacc tggcaggcct actaccgctt cgccaaattt accaagcagt
     8941 gttcccataa aataataaga agcacgcaaa ctattgattt ttccgtgcgg catcggaatc
     9001 gatacgactt ctcgaggatc gatttctagt atgttgttgg aaaaatggac agttgcgccc
     9061 atgatctcta aaatttcaat taatgaatga acatcttgta tgtccggcac accttccaag
     9121 atcactgggg aatcagctaa gattgcagct ggaatcaatg cgactgcact attttttgca
     9181 ccgctgatcg ttacttcacc agctaaaggt cggtttcctt tgattacgat ttttttcatc
     9241 tgtttccacc ttgtttatta atcttttttc atggatatgc ttgtatttta tcatagataa
     9301 aaaaaagaaa cgagcatggt ctgccgtttt aagtaaaaaa gaatggagag cgacttcaaa
     9361 agtcgttctc cattcctata tatcgtatct gttatgtgga tgcttacgct ttgttagctg
     9421 aaccgaacca gtcgatatga gatttagcat cttcaacgat tgctgcttta cctggagcta
     9481 ataatttacg agggtcgaat cctttacctt ctttatcttt accagcttcg atatatttac
     9541 gagtagctgc agcaaatgat aattggaatt cagtgttaac atttactttt gagataccca
     9601 ttgagatagc tttttcgatt tgatcttgag gaatacctga tccgccatgt aatactaaag
     9661 gcatgtctgg acctacagca tcagcaattg cttgtaagtg gtcaaaagct aatcctttcc
     9721 agttttctgg atattgacca tggatgttac cgataccgca agctaagtag tcgatacctg
     9781 tagcaaccat ttgtttgcat tcttcaatat cagctaattc gccgttaccg atgattccgt
     9841 cttcttctcc gccgattgaa ccaacttcac attcaactga gatacctttt gcatgtgctt
     9901 tttcaacaac gtctttagct aatttcaagt tttcttcaaa tggtagatgt gaaccatcga
     9961 acattactga agtatagcct aattcgatac attccaaagc agcttcgtaa tcaccatggt
    10021 ctaagtggat agcaactgga acagtgatgt tcattgcttc aactaaatca gtgattaaat
    10081 ctttacagat tttgtaacca cccatgtatt tagctgcacc cattgatgtt tggataagta
    10141 ctggtgcttg ttttgcttca gccgcttcaa ggattgcttg agtccattcc aagttgtttg
    10201 tgttgaatcc gccgacagcg taaccgcctt tacgagcagc ttttaaaaat tcagctcctg
    10261 atactactgg cataataaaa ttcctcctaa gttttgtaaa actaaatttg ttaagaca
//
LOCUS       DAYYKC010000098        10310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000098 DAYYKC010000000
VERSION     DAYYKC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.099"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHBBW_002435"
     tRNA            8..81
                     /locus_tag="ACHBBW_002435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACHBBW_002436"
     tRNA            101..173
                     /locus_tag="ACHBBW_002436"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACHBBW_002437"
     tRNA            201..274
                     /locus_tag="ACHBBW_002437"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACHBBW_002438"
     tRNA            288..361
                     /locus_tag="ACHBBW_002438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACHBBW_002439"
     tRNA            385..474
                     /locus_tag="ACHBBW_002439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACHBBW_002440"
     tRNA            487..560
                     /locus_tag="ACHBBW_002440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACHBBW_002441"
     tRNA            579..654
                     /locus_tag="ACHBBW_002441"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACHBBW_002442"
     tRNA            656..726
                     /locus_tag="ACHBBW_002442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACHBBW_002443"
     tRNA            740..813
                     /locus_tag="ACHBBW_002443"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACHBBW_002444"
     tRNA            838..925
                     /locus_tag="ACHBBW_002444"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACHBBW_002445"
     tRNA            947..1018
                     /locus_tag="ACHBBW_002445"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACHBBW_002446"
     tRNA            1041..1130
                     /locus_tag="ACHBBW_002446"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACHBBW_002447"
     tRNA            1143..1216
                     /locus_tag="ACHBBW_002447"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACHBBW_002448"
     tRNA            1235..1307
                     /locus_tag="ACHBBW_002448"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACHBBW_002449"
     tRNA            1311..1383
                     /locus_tag="ACHBBW_002449"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACHBBW_002450"
     tRNA            1388..1458
                     /locus_tag="ACHBBW_002450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACHBBW_002451"
     tRNA            1471..1544
                     /locus_tag="ACHBBW_002451"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACHBBW_002452"
     tRNA            1556..1627
                     /locus_tag="ACHBBW_002452"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACHBBW_002453"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACHBBW_002453"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9154091.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACHBBW_002454"
     CDS             3216..4688
                     /locus_tag="ACHBBW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9154092.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACHBBW_002455"
     CDS             4771..5559
                     /locus_tag="ACHBBW_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9154093.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACHBBW_002456"
     CDS             complement(5626..6294)
                     /locus_tag="ACHBBW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9154094.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACHBBW_002457"
     CDS             6409..8121
                     /locus_tag="ACHBBW_002457"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154095.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACHBBW_002458"
     CDS             8124..9896
                     /locus_tag="ACHBBW_002458"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154096.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..>10310)
                     /locus_tag="ACHBBW_002459"
     CDS             complement(9978..>10310)
                     /locus_tag="ACHBBW_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9154097.1"
                     /translation="IIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKE
                     LNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLV
                     EKLGKIEI"
BASE COUNT         3000 a         1820 c         2333 g         3157 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
    10261 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
//
LOCUS       DAYYKC010000099         9944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000099 DAYYKC010000000
VERSION     DAYYKC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.100"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHBBW_002460"
     CDS             32..388
                     /locus_tag="ACHBBW_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM9154098.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHBBW_002461"
     CDS             404..754
                     /locus_tag="ACHBBW_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM9154099.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHBBW_002462"
     CDS             783..1562
                     /locus_tag="ACHBBW_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM9154100.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHBBW_002463"
     CDS             1573..2235
                     /locus_tag="ACHBBW_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM9154101.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHBBW_002464"
     CDS             2254..3360
                     /locus_tag="ACHBBW_002464"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM9154102.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACHBBW_002465"
     CDS             3350..4441
                     /locus_tag="ACHBBW_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM9154103.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACHBBW_002466"
     CDS             4454..5194
                     /locus_tag="ACHBBW_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM9154104.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="ACHBBW_002467"
     CDS             5482..7422
                     /locus_tag="ACHBBW_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9154105.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="ACHBBW_002468"
     CDS             7509..9431
                     /locus_tag="ACHBBW_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9154106.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9944)
                     /locus_tag="ACHBBW_002469"
     CDS             complement(9711..>9944)
                     /locus_tag="ACHBBW_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM9154107.1"
                     /translation="DLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQ
                     AVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3247 a         1651 c         2207 g         2839 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaatca aatc
//
LOCUS       DAYYKC010000100         9676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000100 DAYYKC010000000
VERSION     DAYYKC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.101"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..3321
                     /locus_tag="ACHBBW_002470"
     CDS             43..3321
                     /locus_tag="ACHBBW_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9154108.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3862..5769)
                     /locus_tag="ACHBBW_002471"
     CDS             complement(3862..5769)
                     /locus_tag="ACHBBW_002471"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9154109.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5899..6774
                     /locus_tag="ACHBBW_002472"
     CDS             5899..6774
                     /locus_tag="ACHBBW_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9154110.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6854..7828
                     /locus_tag="ACHBBW_002473"
     CDS             6854..7828
                     /locus_tag="ACHBBW_002473"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9154111.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8008..9153
                     /locus_tag="ACHBBW_002474"
     CDS             8008..9153
                     /locus_tag="ACHBBW_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9154112.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9290..9634
                     /locus_tag="ACHBBW_002475"
     CDS             9290..9634
                     /locus_tag="ACHBBW_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9154113.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3151 a         1794 c         2048 g         2683 t
ORIGIN      
        1 ctttaagtaa acgctttcta atttgttacg ggaggaatac acttgttaaa aaaacgtata
       61 aaatacagtg cgctaatgat gaatctcctt atgctcagta ccccggtttt aatttctgtc
      121 gttgaagttg ctgctaacga acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt
      181 gaagggatgt caacaatgga ggaaatacca agaactagtg aagatatgga tacggaagaa
      241 ggaaatgatc ctatgatgaa agaatcagaa aatgaaaaga caaaaaagca atctgaagaa
      301 gcaaatgaag agtggaaagg ggttgttttc ggagaaagca caagtgctag taggtatgcc
      361 attgaaccat tagaagacgg cgtgcggctc tcttccacga ataacggcgg aaaattccag
      421 tcaagcggat cagatggaat gacctattat tatcaagcaa tccccaaaga catcaacttt
      481 aggatgcggg cgacgatcga aatagaatct tggacctata cgaatgccca agaaggattt
      541 gctttaatgg taagagacgc agtaccaaaa aatcatttgt ttgggcaggc attttattca
      601 aattcatttg ccatactagg cagtcggatc gaatatgtct gggactcgga tagacaagaa
      661 gtggtcaaca cgagcggcag caaatatacg atgcgacttg gattggggac acgggcaatc
      721 acaggaatat catctgaaga tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa
      781 acaacacctt tagagacttc agcagggttc ttggaaaaag aaacaggttc ttataatata
      841 ttcggaaacg gtcatgggaa tctgtttccg gccacgaact cgattactaa actggacgta
      901 gagatgaaaa aaacgaatac tggtttagag gcatattatt acgatcctga aacaggagaa
      961 gaaatggctt ctgacatcat gtatgactgg gaaaaattat acgcttcaga tgaatcggtt
     1021 atttatgcag gatttgctgc agcaaggaat atgacgatca aggtagaagg aatcgaagtt
     1081 gcttactcag atcctgcgac agatcctcct gggcaagaaa gacctactcg cttgattgat
     1141 ccgatttaca ctgtgacctc tagtaatcat tcaggaaacc aagatcatac tatgtccttt
     1201 agagcaaatg ctgacggatt attgacaatt aaaaataaag caacaggaga ggtgcttaag
     1261 aacgcaaaaa acttggctat cactgcgaat cgggaatttg attatcagac aaaactgatg
     1321 gaaggaacga acgaatttac tttcagtttt acgccagata aaaactatcc gccagaagaa
     1381 tacgtagaga tgactagtta tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa
     1441 cctaaatatt cgacagttta tgtaacacca gaagggtcag atgcaggaaa cggaagcaga
     1501 caaaatccat tatctctgac acaagcattg agatacgcat atgctgggca gacaatcgtc
     1561 atggcgcctg gaacttattc cttcgaaaga ggattgacga ttccaaaagg cgttaacggg
     1621 acaagagaaa acccgattca gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta
     1681 cttgatttta aaggaactgg aaatgggatg acactgttca gtcactactg gggtttacga
     1741 gggtttgaca ttacaaatag tgcagctatg caaaaaggat tacagatttc tggaaatcat
     1801 aatttgatcg aagagattca tgcttatcgg aatggaaata caggaatcca aatcagcggt
     1861 agctcaaatg atcctttcga agcatggccc gcgcataact tagtcaagaa ctgtacatct
     1921 tttgaaaatg ccgatccagg atttgaagat gccgatggat ttgcggccaa actgacaata
     1981 ggagaaggaa atgtatttga cggttgtatc gcgtatcata atgcagatga cggttgggac
     2041 ctttttgcta aaaatgaaac aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct
     2101 tatcgaaacg gctgggtacc aggaattgag ggagaaggta acggtaatgg attcaaaatg
     2161 ggcggttctt ctttaacggg ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt
     2221 gcgaaaggaa tcgattccaa tagcggaaca gatattatcg tcaatagcag tacaagtttt
     2281 aataacggtt catataatgt ggctctttat acctcttcag caggaaacac agactaccat
     2341 gcttctggta tcttatcttt ccgaacagag aatcttgaga tgagagagca gatccgccca
     2401 ttgaatcaaa acgaagatca aatctaccac tcgttgaact actattggaa tgaacgagag
     2461 aaacaatcag aaaatactct tggacatacc atatcaaaag attggatcga gtctctaaat
     2521 acatgttcga aagaaggtca gcagcctgtt acgcgctatg aaaatggaag tatccatgtt
     2581 catggactga tgcagattaa accggggaat cgtcaaacag aaaaggaaag agtggatggt
     2641 cttccattat ctgtaggtgc tatttttgac ggagaaacct caccgtcttc ggaatatcca
     2701 gagtatgcaa tcgtacaaga accaaatcag acaaaaccaa atgaacaaaa taatccgatt
     2761 attatacaag aagcaaatgg tgtggcggag aatgcagaag gaaatatcat tgaactgaag
     2821 ccttttacag tttttgtgtc aactttcaca ccaaatcaag gaacgcctgt ctttgaagat
     2881 atgcgttatt tcccagaaaa gatccaaatc gttgaaacac agtcaagtgc tcaattttct
     2941 aatcataccg ctattttttc agcaccttca aaaggagaat atacgctaga agtcacttat
     3001 cgttcggaaa tatttgatgg ggaaaaatgg tctgctcata cagaatctgt tacccagcaa
     3061 aaagcgatca ctgtgcgaga aactgccgat gtgcccccgg gaaatgacaa accagaaaga
     3121 gaaaatccag gaaatgaatc accaaatgga aaaccaaatg gaaaaccttc tcatccggat
     3181 aggacagcaa aagaaagtga aaaaaatggc actcaaggac tcccaaaaac gggagaattt
     3241 aataacccgt tgctagcatt agctggagga attttgttaa tcggtgtagt tgtttatgtc
     3301 atgaaacagc gaaaaaaata agtggaattt ctccttttat gatgtaaaac aattgactga
     3361 ggaaagtaaa aatagaagga aatgagacca atagatgtct cacttccttc tatcttttta
     3421 tagatgaggt tctccttgct gcttttgatt ggctgttctt attacttgat gcaggacagc
     3481 tttttcacaa tctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac
     3541 aaacggcaaa atatgaggaa ctatttttcc gtttttttct ctaatactca ggtcaggacg
     3601 ctgatttcac aacctctcga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
     3661 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttactact tgaaactaag
     3721 cagctttttc acaacctcta atacatggta ttccagaagt aacttcttcg aaaaataagc
     3781 cgaaatgtca caaaaatttg agaagcgatt ttcccaaatt ccttcttatt tcttgaagct
     3841 gaccacttct ggcacaacct cttaccagat tcgcactctt ttttctggcg ctagatacat
     3901 agcgtcttgt tccgtgatac caaatgtttc atagaattcg tcaaggtttt ttggttgcac
     3961 gtttgctcgg agtttagcag gggcatggac atcgatttgc agtaaaagtt gttggtattc
     4021 tttttttgct tttgtacgcc aaatcgttgc ccaattgaca aagaattcct cgatagaaag
     4081 atctgattcc gtttttgctg cttccaatgc acagcttaga ccgcctgcat cagcaatatt
     4141 ttccgaaacg gttaattttc cgtttacttt tccgtcagca aaaggcagac catcgaattc
     4201 ttcgatcatt gcttgtgctt tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt
     4261 gttcaagttg ccatattcgt caaataaagc gccgttatta tcaaatgcat gggaaatctc
     4321 atgagcgata acagctccga ttccaccata attagcagag cttgattgtt ctagactgta
     4381 aaatggtgct tgaaggatcg ctgccggaaa gacgatcaca ttccgaaatg gatgataata
     4441 agcattgacc gtgtcagcac tcatttccca ttctgaacga tccactggtt tgttccattt
     4501 gctgaaacga tcttttaatg tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc
     4561 accctgatct gcaggtactg ttttgaactg gtcgaataat gctgggatct tatcggggta
     4621 gcctacttgg atacctaagt tatttaattt tatgacggct ttttcttttg ttgcatcccc
     4681 aagccagtca ttttcttcta gacgtttttt ataaacggcg atcatttttt tcaccatttg
     4741 ttctacgtct tttttagcag cttcgccaaa atattttttt ccgtagtaat cgccaactac
     4801 ttgatcgaat tgtccagagg caaggtaata cgctgctttt ttctgaggca ttgcttcgtc
     4861 tgttccagac aatacgcgag aatagattcc gctgacttgg cggaattctt ctgatagata
     4921 accactaaga gaacgtactg ttttaatgat catccagctt ttgaataacg gaaaatgagc
     4981 ttctgtcagg atgtcattca aatgatcgaa gtacactgga tcagttacga tcactttatc
     5041 tggagttgtc ccaatcaaac ccgtgacaag ttttttcaag tccagtttat ctgtgtattt
     5101 aataaatgta tcaaaagatt gcggattata cattttgctg taatctgcgt tttcttctgc
     5161 actttttaca tgaggagcaa tcaatttatc aaattgcact gcttgttccg tgattgcttc
     5221 tgcctgactt ttttctttgc cggcaagctc taataactga acagccatat caaagaaaac
     5281 gcctattagt tgtgcaccgt tgggatggtc tgatgcataa tacgtcttat ctggaaggaa
     5341 taaagaaggc ggataagcaa agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac
     5401 atccagatta aatggcaatg gtaatgtatc caacagccat tctggaaatt gcttattcaa
     5461 ttcgtcgaaa gactgcaatg attcaacttt ttcgattata ggttgaagcg gttgagcatg
     5521 caactcattt cttcggtcat aatcgtttgc cagttgataa tatgcaagga aatgtttcat
     5581 tttttctgaa gacacttttt ctggatgggc aagcagatcg tctgtttctc ccatcaaaag
     5641 ttggtcgatc ccgtcaacta aatcttggaa accgcctgta gctggtttat ctgcagggat
     5701 ttttgctgtt ttttgccatt cttcattgac tgcttcatag aaatcttgtt tcaatcgttc
     5761 tttgttcatt catcgatcgc tccttttttg tttcgtgtgc ccattatctt acaaaatgaa
     5821 ggaattaaca aatagaacat aaaaaattga aaattcgaac aatcaaactg atttgttttt
     5881 ttcaatcctt cacttataat gaaaaaaagg aggcgatccc aactgaatca agtagtggat
     5941 aaacctttat atttcaaagt agataaaaaa attaaaaaat tagccagcac tcagcagctg
     6001 cagtcaagaa aaagtaaatt gcttttttta gctctggttt ttgaagatca aagttatgtg
     6061 attatcgatc aatcaggaca tccggtagaa tattctccag caaaatatac ttatcaagag
     6121 ggtctttctt gtaaaaaatg gaagctgatc aatgaagagc caatcgagtt atctagatgg
     6181 atcaacagaa aagaagatat tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc
     6241 gcaaattgct gggtgggatt gccagaagaa cgttttttga ggtataagaa atgggctacc
     6301 ccctcaggct atctttgtgg tacttacgcg gcagcagttc tccttgccta ttatcaagac
     6361 tatcgtaagg ggtggatgct tccaaatgag attcgcaaga aaaatacggc tgattcgttg
     6421 gttttgacga aggcattgcg tagccagatc caaccactag gacttcctac gattcctttt
     6481 caggttagta cagggatttc tagtttttta aagaagaatg gcaatcatga gcgtgcacga
     6541 gcaactcttc taggttcatg gcagcgagct acaaaacgta tacgtgaagg caaacctgtg
     6601 atgattggta ttttgaaagt acttggcagt acatatggca atcattgggt gacagcgtac
     6661 gcttattttg aaactgaaac aggagaacgt tattacaaag tacacgacaa ctggggcgat
     6721 tatcataaag tcattcctgc cagttggagt aacggaacag tttcacttcc ttaagaattt
     6781 gctgaaatta acagaaaatc ttggaaaaat atgttaaact atcgaagaat agagaagttt
     6841 tggggaggac tacatgagta ataagtatca ggatgacacg ttgaagattt ttagtttgaa
     6901 tggcaaccgt ccattagctg aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag
     6961 cgtcgtgaag caatttagtg atggcgagat ttccatcaat atcgaagaaa gtattcgagg
     7021 ggatcatgtg tatatcgttc aatcaacgaa tgcacctgtc aatgattact atatggaact
     7081 actgatcatg atcgatgcaa tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc
     7141 ttactatggg tatgctaggc aagaccgcac ggcgaaacct catgaaccga tcactgcgaa
     7201 attgatcgct aacttgatcg aagaagcagg ggcaactcga gtactgactt tagatctgca
     7261 cacggttcaa gtacaaggat tctttgatat tcctgtagat aatttattta cgatgccgtt
     7321 gtttgctcat tattaccgtc aattaggtct gacgggagat gacatcgtag ttgtttcgcc
     7381 aaaaaacagc ggtgttcagc gtgcacgtag tctttcagaa tatttgaaca gcacattagc
     7441 catcgtagat catgcagatg atgcttcaac tgagggtggt tatgtgatcg gtgatgttca
     7501 aggaaagaca tgtatcatgg tagatgatat cttgaacaca ggagcaacgc ttgcacgagc
     7561 agcaaatgta ttaaaagaaa acggcgcaaa agaagtatat gcctgtgcgt cacatggatt
     7621 attgtccgat catgcgaaaa gtattttaga caatgcacca atcaaagata tttgtatcac
     7681 tgattctgtt ttcacagaag aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc
     7741 agaattaatg ggtgaagcgg tcaaacgcat ccatgaaaat acaccgatga gtccgttatt
     7801 ccgtctggaa gaaaaagaat tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga
     7861 agtgagcagc ttcaaggagc tgcgacaaga cttttgtcac agctcctttt ttctatgccc
     7921 atgaaacgtc ctctagacaa aaatgagctc ttctttggtt tgttcgctat tttatgctag
     7981 aatgtgccta gcaataattt ggaagggatg aatgaagtga aaactattta tctggatcat
     8041 gcggcaacta cccccatgca tccacaagta attgaagcga tgacggcaat catgcaagac
     8101 acgtttggaa atccttcaag catacatgga tttggtcgcc acgcgcatga aaaattggaa
     8161 acagcaagac aaatcattgc agacagtctg caagcaaaac ctcacgagat catctttaac
     8221 agtgggggaa cagaagggga taatacagct atcttagaaa cagcgttctc gcgtcaaaaa
     8281 gaaggcagac atctaatcac tacagctatc gaacatcctg ctgttcttaa taccatgaaa
     8341 tatttagaga caaaggggtt tgacgttaca tatctgcctg tagatgaaaa agggaatctg
     8401 tcgatcgaag aggtcaaaaa agcgttacga gaagatacga tcttggtctc tgtgatgtac
     8461 gggaacaatg aaatcggaaa tttgatgcca atcaaagaaa tcggtgaact tttagcggat
     8521 catccagctt atttccatac agatgcggta caagcgtacg gaaaccaaac gatccatcca
     8581 gaagaattag gaatcgattt attaagtatc tctgctcata aaatcaatgg gccaaaaggc
     8641 gtcggtttct tatataaacg agacggagta aatatccctg cattgctttt aggcggggag
     8701 caggaagaaa agcggcgtgc aggtacagaa aatttagcag ggatctgggg tatggcaaaa
     8761 gcagttgaac tgctgtcacc tgaagaacgt gaagcacgta acaacaaata taaaggattc
     8821 cagcaattga ttcttgatac attagaagca aacgaaatcc cttatcaagt aaatggtgat
     8881 ttaacgaata aactccctca tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg
     8941 ctactgatga agcttgatct taatggggtc gctatttcta ctggttcagc ttgtacagca
     9001 ggaaatatcg agccttcaca cgtattagaa gcaatgtatg gagaagatac accgatacta
     9061 aaagaatcca tccgtatcag ctttggtttg ggaaataccg aagacgacat ccagtatttc
     9121 cttgatcaat tagttcgtgc tgtcaaaaaa taactagtga agtcattctt atatgaacag
     9181 atgtctgaga gaacgccttc attttttagg gaacttaact gaaaaaccta gtaaaaatca
     9241 ggataaactt ttataataaa gagaaactaa ttttatcgag gtgaaaatca tggcatttga
     9301 aacaacagca acggtactgg gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag
     9361 atatacattg cgcgataatg gatttacaga aacaaacgga ggcaattatc aattgatccg
     9421 accattagat gccacgccgc aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa
     9481 agatattcaa acattgaaaa tgtcgatcac tacagcaaat ggattgaaag cagtcaatat
     9541 tttcaaagat ccaaaacaga aaatgagcca agataaattt tattttctga tggatgggat
     9601 gattagccgt gggttattcg aaaaagtcga atgatctgtg gatataaaaa gacaactagt
     9661 tttgaaacgg cctatt
//
LOCUS       DAYYKC010000101         9635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000101 DAYYKC010000000
VERSION     DAYYKC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.102"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..981
                     /locus_tag="ACHBBW_002476"
     CDS             <1..981
                     /locus_tag="ACHBBW_002476"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9154114.1"
                     /translation="KEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYL
                     FDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTI
                     CLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLN
                     EFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQW
                     INSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQA
                     DFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            963..1424
                     /locus_tag="ACHBBW_002477"
     CDS             963..1424
                     /locus_tag="ACHBBW_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9154115.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1474..1938
                     /locus_tag="ACHBBW_002478"
     CDS             1474..1938
                     /locus_tag="ACHBBW_002478"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9154116.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1952..2701
                     /locus_tag="ACHBBW_002479"
     CDS             1952..2701
                     /locus_tag="ACHBBW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9154117.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2701..3531
                     /locus_tag="ACHBBW_002480"
     CDS             2701..3531
                     /locus_tag="ACHBBW_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9154118.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3531..4178
                     /locus_tag="ACHBBW_002481"
     CDS             3531..4178
                     /locus_tag="ACHBBW_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9154119.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4276..5052)
                     /locus_tag="ACHBBW_002482"
     CDS             complement(4276..5052)
                     /locus_tag="ACHBBW_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9154120.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5196..6155
                     /locus_tag="ACHBBW_002483"
     CDS             5196..6155
                     /locus_tag="ACHBBW_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9154121.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6272..6580
                     /locus_tag="ACHBBW_002484"
     CDS             6272..6580
                     /locus_tag="ACHBBW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9154122.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6607..6906
                     /locus_tag="ACHBBW_002485"
     CDS             6607..6906
                     /locus_tag="ACHBBW_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM9154123.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7080..7631
                     /locus_tag="ACHBBW_002486"
     CDS             7080..7631
                     /locus_tag="ACHBBW_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM9154124.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7777..8070
                     /locus_tag="ACHBBW_002487"
     CDS             7777..8070
                     /locus_tag="ACHBBW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM9154125.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8063..8359
                     /locus_tag="ACHBBW_002488"
     CDS             8063..8359
                     /locus_tag="ACHBBW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154126.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8430..9434
                     /locus_tag="ACHBBW_002489"
     CDS             8430..9434
                     /locus_tag="ACHBBW_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9154127.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3415 a         1378 c         1799 g         3043 t
ORIGIN      
        1 aaagaaatga gacaactaca caaagaacag attgaaacag caatggcagc gttaaaggaa
       61 aagacaattg atcatgtata ttttacagca tgtgggggtt cacaagccgt tttaatggct
      121 ggtcaatatc tatttgataa agaattaact gtaccttgtc atgtttatac tgctaacgaa
      181 tttaactatg ctataccaaa aggattatct gaaaattctt tagttatcac ttgttcccat
      241 tctggcacaa caccagaaac tgtagaagca acaaaaaaag caagagaatt tggagcaatg
      301 actatttgtt tatctaatga agaaggatct cctctatggg aagcagctga atatcctgtt
      361 cactatgatt ggggtaaaga tgttgatgct agtgatttaa ataaaggaat tctttatggt
      421 ttattattta atattttact tgttttagat ggctctaaaa aatgggaaac ttgcttgaat
      481 gaatttgata agttaaatga tcttgttatt gctgaaaaga gcaaatttga aataactgct
      541 aaagaatggg gtaaaaacca aaaacgagat aaaattattt acacaattgg tagcggtatc
      601 aattacggag aaatttactc cacagcagct tgctggttca tggaaatgca atggattaat
      661 tctaatgcta ttcattctgg ggaatttttc catggtcctt ttgaaatcac tgattatgat
      721 gtaccattca tcttagtgaa aagtattgga gatacaagat ttttagatca acgggtagaa
      781 gacttcacta aaaaattcag taataaatta atggttttag accaagctga ttttgactta
      841 gaatctatag atagtgtagc gaaagaatat attgcagctg tacttagtgg cgttgttatt
      901 cgccaaatgg tagaagcgat tgcttttgaa agaggccatt ccttagatgt tagacgctat
      961 atgtggcaaa tgaaatatta attaatagga ggttgcaaaa gatggcaaat caattatttc
     1021 acattgtgat tacaacacat tctacccttt gtgagggtta tttaaaggca acaaaattaa
     1081 ttttaatgaa tgacgccaca ggtattagtg ctgttccatt taaagaaaat actagtccag
     1141 atgattttga ggagagtatg aaagaaataa ttagtcagca tcatttgcaa cctctaatta
     1201 ttttaacgga tttaattgga ggaacaccta ataatgtttc tattaaacag ttgaacaaca
     1261 atagaattca aattattgct gggattaatt tacctctttt actggagtta ttaatggccc
     1321 agtcagctgg taaatctata gaagaaatag attttaagga attactgaat aactctcaaa
     1381 aaagtatggt tcatattaat tcgcttttaa aagatgtcag ttaaggtggc aatttctatt
     1441 ctataaattt taaaaattga aggaggaaat agaatgatta aattagtgcg tgtagaccac
     1501 agattacttc atggacaagt aatattttct tggactaaac agtatgatat taaccatatt
     1561 attgtggctg ataacaaagt tccaggagat ccaatgtctg tgatggcttt gacaattgct
     1621 aaacctgctg attgcaaatt agacattgtt aaaattagtg atgttaaagg gattatggaa
     1681 acatccccaa atgataatta catgatttta attaaaggac ctaaggaagc ttttgaatta
     1741 gttcaaaatt taccagaaat taaagaaatt aattttggcg gtgtgtctaa gaaaagcgag
     1801 tccaaacaat atggaaaggc agtcttttta actcctgaag aattagaagc cacaaaaaaa
     1861 attcttgatc aagaagtgaa aatatttgtg caacaagtcc catcttcaag tattgaaaat
     1921 gtagatttta gtaagtaagg ggggaaaata aatgttatta aaagccattt tattaggttt
     1981 agttggtgtg gtagctgtta ttgactccag gttaattggt cgacaaaata ttggtagacc
     2041 actaattttg agtacacttg ccggacttgt gctaggtgac ttgcatacag ggattgttct
     2101 tggggcatct ttagaattaa tttctatggg attcgtctca attggggcgg ctggacctcc
     2161 taatatgcaa cttggtagta ttattgccac tgcttttgca attttaacta atagtagtac
     2221 agaaacagcc ttaacaatag ctatcccagt ggctgtagct ggtgaattta tgagtattat
     2281 catgcgcatg ataattgctc aatttggaca tgctgcagat aaagccattg aaaataataa
     2341 atttagaaaa gctcaaatta ttcatattta ttggtcattt atttttaatg cttttgttta
     2401 ctttattcca atctttttaa cagtatattt tggtgctgac gttgtggcag atttagtaga
     2461 taaaattcca gaaatgatta cagacgcctt gactgtctct ggaaatatgc tttccgcatt
     2521 agggtttgca atgctattaa gcacaatgtt gagtaaaaaa ttatatcctt actttatttt
     2581 tggattcttt attgttgcct attcaggtct ttcactaatt ggcgtgacaa tttttgcagc
     2641 tctgattgcc tttgtaatgg atcaagttaa atatggaaaa agagaagagg cgcatggtta
     2701 atggaaaata gtacagaaaa aaaacgttta atcacaaaaa aagatcttaa taagatgtgt
     2761 cttcgttcat ggacaatcat gtgttcgtgg aattatgaaa gacaaatgca tatgggattt
     2821 atgtatggga tggcgccaat tttaaataaa gtttataaaa atgatgaaga gaaaaaaaga
     2881 gaagcttata ctcgtcatat ggcatttttt aactgtacgc ctcaaatgac ttcatttatt
     2941 atgggacttt gtgcttctat ggaagaacaa aatgctcaag cagaagaagg aaactttaat
     3001 actgaatcta tctccatgat taaaaccagt ttaatgggtc cttttgctgg aattggtgat
     3061 tcatttttcc aaggtacttt aagaattatt acatttggga ttggattgtc ttttgctaag
     3121 caaggtagta ttttaggacc gattttagct gttggccttt ttgctatacc ttctatttta
     3181 gctgtttttt acggcggtaa attaggatat gagtctggca ataaatacct tactaaattg
     3241 tatcaagaag gtcttatgga tcgagtaatg caatttgctt ctatcattgg tttggctgta
     3301 gtaggtggaa tggtggctag catggtgcca attgtcaccc ctttaaaatt cactacaggg
     3361 ggaacagaac ttgttttaca agacttactt gattccatta ttcctggatt attaccactt
     3421 gtatgtactt tgggaatata taaattgatt aagaaaaatg ttagtacaaa tatgctctta
     3481 ttaggttgta ttttggcagg gtttgtttta agtataatag gtatcttata atgattaagg
     3541 ctctaatttt tgatatggac ggagttttaa ttgatagcga gattatgtat caaaagtggg
     3601 tatttaattt tttaaaacaa gaaaatttaa gctatccttt gtattcttat caaaaaaaga
     3661 ttggaactac agcaagtatt tttgatgata tagaaaagta caatgaggga tgtaacagtt
     3721 tagaattgaa gggaaaattt actaaatatt ggaaatcgca aaagattgat tacttggata
     3781 tttttcctaa tatactaatt gaacagcttg aatggttaaa aaagaaaaaa ataaaaatgg
     3841 caattgcttc ttccagtcca atggatacta taaatgaagt tgttaccact tgtaaaatag
     3901 agtcatattt tgattgttta attagcggaa gagatttgcc agaaagtaaa cctaatccta
     3961 ctatattcct taaagcttca gaacagctca gaattccagt tgaagaatgt ctggtgatag
     4021 aagattctta taatggtata aaagctggta aaagagcaaa tatgaaggtt cttgcaatta
     4081 aagataaacg tttttctcaa gatgtaagtc tagcagatga tgttatttac gatattaaat
     4141 atctgcgaca agaagtacag gtgaaatttg gaatataaat aagttttttg atgtatttag
     4201 agaatattag taaaaataaa aatcatgccc aaatctaatc aaaattgatt gctttgggta
     4261 tgatttcttt taatatcaat ctattacttt aggatctgaa tcatttttat gcaccgtatc
     4321 taaagtgata ttttctgtga cttcaataac ataactaatt ctatcacaac gaactgtgga
     4381 ttgagaataa tgaaatggtt gattattttt atctttgcca accttttcta aaataaatag
     4441 cggatctcct actatacatt gcaataagtc ggaaagctca ggtgttgcag ataaaccgtt
     4501 tatttccata atagagctcg tagctttgac atcatagtca tcttctagaa gtttatataa
     4561 tgaagtatat tggctacctt tttcaaataa agttggaaat ttttcttctg atgcgaaaaa
     4621 attatcaatt cccatcgggg caccgtcttc ccacattaaa cgtttaatat tgtaaactct
     4681 atcagttaca ttgatttgta atttattagc taaaaactca tcagccttta taattttaga
     4741 atgtaaaaca gtatatctaa ttttatgtcc tcgggtagaa agagaatcgc taaatccatg
     4801 aactccagct aattttaaac tttctttttt ttgagttaca aaggtccctt tcccccttaa
     4861 tcgttcaact gttccacttt ctgctaaaag tttaaaagct tttcgaatgg taatcttact
     4921 tactccataa attttacata attcttcttc tgtaggtaat tgctctcctg gaagatattt
     4981 tttttcacga atatcgttac gtatagaagc ttctacttgt aaatataatg gtctattatc
     5041 ttgaaaattc atagtacact ccctaaaaag ttatacttca ttatatcaca atttattaaa
     5101 acgtcgattg taaaattaag ctagacaact aaaaagatca tttgtgctac actcaaaata
     5161 gttccgacca aagaattaat aaggagtgag tacaaatgac ttacacccat cttacaacag
     5221 acgaactagt aatcatagag tcttatttca atatcgagaa acctgtcgct cttgtcgcac
     5281 aaagtttaaa tcgctctaga caaaccattt ataatgttta ttcgtttctc aaacaaggaa
     5341 aaaaagtgct agattattac cgtcaataca aacaaaataa atctcgttgt ggacgacgtt
     5401 caaccgtttt atctgatgaa caatcaacat atgttcaaga aaaggtagct caaggatggt
     5461 cacccgatgt gattatcggc cgtggcgagt ttccaattga ttgttctgtt cgtacactct
     5521 atcggatgtt taaaaaaggc ctttttgaca cacagtcgtt acctatgaag gggaaacgta
     5581 aaccgaataa tcatcaagaa aagcgtggaa aacaggcctt tcgcagaacg attcatgaac
     5641 ggaacagtct ctatccacac ctgactaaag aatttggtca tctcgaagtc gacaccattg
     5701 tgggagttaa acacaagagc gctgttatta cattggttga gtgtttatcc aaggttatta
     5761 ttactttgaa accgacaggc agacgagccg aagatatcga aaacaggtta aatgattggt
     5821 tcaaatccat gcctaaacac ttgtttaaat ccatgacatt tgattgtgga aaagaatttt
     5881 ccaactggcg atctataagt aactcgaacg atattgccat ctttttcgca gatccaggaa
     5941 caccttcgca aagagggcta aatgagcact caaacggctt gcttagacga gatggcttac
     6001 ctaaaacaat ggactttagg gacacggatg aaacctttat tcaatccgtt gcctcgaaaa
     6061 gaaaccatat tccaagaaaa tcattaaatt atcgaacacc attggaagta tttttgagct
     6121 gcatagataa agacatattg tctagcttaa tttgacaaat cagatagtat aaaatgaatc
     6181 aaattgaata tcaaatatat attatatcca tgaagcccaa tcttctttaa tagaagattg
     6241 ggctattttt atggcaaagg agggaaaaga aatgtttcaa aataaaggac aacgttatgt
     6301 cacaaaggga gtaatggaca gtttgccggt ggaattacaa gctttatgtt ggaatctaat
     6361 cgatcaaaac gttcaaaagc aatttcattt agactatctt caaatctttg aattttctac
     6421 ggaaaaagga aatcaaaagt tggttcatcg tcaagaagaa ccagaagcaa gaaaagagta
     6481 tctgatttca cctaagcttc gtttgaaatc agtgtctcaa aaaatatggg tcattgattc
     6541 gggtgagtat caaacgatgt tattaccaga agaatactga tatctaaaag aaaggaacga
     6601 aaggacttgt tagaaacggc tatcgaagta ctggaaaagt gtgcacagct agtcactact
     6661 cctgaagagt ggggctatga atccgttacg atggaaaaag aggaaattga aatgggtatg
     6721 cttcctgaag atgttactct accacgttta gtgatgacac atctttatat ttattgcgca
     6781 ccagaagatg gaaaagacta tgtagtttat ttcattacag atattaccag ccagcgagaa
     6841 tttgttcgtg ggttactggt agaaggacga ttggtttgga gtcagattgg aggaacgaat
     6901 gaataagatc gattatttga gaatgattgc gaaatgttta gaagatatcc ttacagcgat
     6961 tcatcaaatt ctgaagaaaa aaggagaaag aatatgaaat attttcaact agtattgaca
     7021 cttataacgg caatggtgat tgaattgctt gaacaaaaat tagcatagga gaaaaagaga
     7081 tgattgaaaa agaattagtg accacacaaa cagaagtttg gcgagatcct catcataccc
     7141 atcgctatgt tttcaaacga caatgggcga tcaaaggaaa agaagcggaa gataaattag
     7201 cggtagtcat tactattcgt ccaacagata cagaaccatt tacgaatgat ttgagtatgt
     7261 tattaatcga aaagaatatc cgacaattag gatttactgg ttttattgct gtgaatcttt
     7321 tttcgtatac aaaagcgaaa agtcctcggg tatttcctcg tggaaatgat gaacaatctt
     7381 tggaaatatt aacaactgtt ttcacagaaa agaaaatcaa caccattatt tttgcttgtg
     7441 gttcgattac agcgacgagt caagtggctt atgaacaagc gaagaccatt tacgatcaat
     7501 tgagtgcgaa acaaaaaaag atgaccaaag tattgataaa tgcagaaggt aagttatccc
     7561 acccactaag tattcatgtt cgtaatgaat ggcaattggc agatagtcaa ttattatttc
     7621 aagaaaaata acattttatt ttcttatgtt acttataagt agttttacat gctttttgac
     7681 tttaaaaata catgtgatga tacatgttat atgaattatc aatgtaccca gtaatatgct
     7741 attttcatta tgtaaaaaat aacagggggt gtgtagatgg agattgatga aacttgtttg
     7801 agtaactctt ataaaagtat gaatgacttt tttgaggaaa tggaactgga ttcagaattg
     7861 ctctatcaat tttatcttgc ttttcgaggg caacagattt cttttccaat gaaaatgtac
     7921 gatcgtgagt tagttagaaa aagaatcgaa aacatgatag aacaggaaaa aactgtagat
     7981 atccgtcaat tgacagaagt ttatggtttc tctgcaagat ggattcaaaa agtaattaaa
     8041 caaaaagaaa ggagacgagt acatggctaa gctgattgta ttaggtggtt taatttacgg
     8101 tggatattgg ttatataaat atatgcttgc gcatccgaat atagataaag gaaaagtaat
     8161 taagacagtt ggggtaatca tagttggact catagctcta actaagaaaa ataatactaa
     8221 tagcccaaaa ataatgagaa cttatcgctg tagaggatgt ggaagggaaa tttctactag
     8281 ttttaatcca tctgaaagcc ctcaaggaag agatcgttgt catgctggct atcgacattc
     8341 ttgggaaagg attaactaaa tgaacaccat tgagattaat tgactgataa aataagtacg
     8401 cgagggagaa attgaaaggg ggaaataaaa tgaaaaaatg gttgttatta ctaattggaa
     8461 tggggatttt attcactggt tgtacatctg agaatacgag gactaaagag actgtagaaa
     8521 ctgatacaac aattgaggct aaaaaaagta aaacgcaatc aactgaacaa actacaacag
     8581 aaatgaaaca atctgtggaa gcaagtacaa gtgaaagttc aatcattgaa tcttcttctg
     8641 aagaggttaa aaatactttg tgggatagtg agaaatctac tcaactagca atttttgtcc
     8701 cacaatgggg aaagacatta ggtcaagaat ataaaagcta caatcctcag aacaatgtaa
     8761 gtctgtatgg tacgccttta ccaagtgcag ttattgatgg aaattggaaa atggcaatca
     8821 atgaagcacc agttaccgta caatggtcag aagatggaac tggtcatgca gatttcaatt
     8881 tagtagcagt ttattctgat gtagaaactg gagaatattt agggcagcat gtatatttct
     8941 ttggctttca aaatggacaa cctaaagttt atttaacgca acaaaatcaa ggcaatgata
     9001 aaaactacat gtacttcaat gaaactcaga accaacagtt aaaacaaggt ttcattgata
     9061 ttgtgaatgg acaaactcct caaactccag tagtagaaga atctactcaa caagcaacaa
     9121 ctacaacgac acaatctaca agtagctcag caatttcaag ttgggaagct gctaaagaaa
     9181 ctgtactaaa taataaagct ttatggttgt atgattctcc tgataaaaat gcatcaaata
     9241 ctaatttaat tagcacaaat gaatatatga aagatctaat gcaagatgaa aatggaagct
     9301 actattcaat taatattgta acggatgata atggaaatac aacaatggct ggtggaaaac
     9361 aattccgtgt ttatacagat ggaagaattt ctcaacgtat tggaatgact aacaattttc
     9421 aaatgattaa ataattgaaa atattattta tcaaagaaaa actgattttg agaaaaatta
     9481 tttaattttc ttagaatcag tttttttata aagtaaaaat gcgaaattaa gtagatgaga
     9541 gtgatgatgt tcgaaatatc tattattttt aataagaata cataagctat ctagtttgta
     9601 aacaatgaga gaaaaaacat caatattttt tatca
//
LOCUS       DAYYKC010000102         9290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000102 DAYYKC010000000
VERSION     DAYYKC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.103"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..689)
                     /locus_tag="ACHBBW_002490"
     CDS             complement(21..689)
                     /locus_tag="ACHBBW_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9154128.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            823..1110
                     /locus_tag="ACHBBW_002491"
     CDS             823..1110
                     /locus_tag="ACHBBW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154129.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1526..3064
                     /locus_tag="ACHBBW_002492"
     CDS             1526..3064
                     /locus_tag="ACHBBW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9154130.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3061..4026
                     /locus_tag="ACHBBW_002493"
     CDS             3061..4026
                     /locus_tag="ACHBBW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154131.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4013..5335
                     /locus_tag="ACHBBW_002494"
     CDS             4013..5335
                     /locus_tag="ACHBBW_002494"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9154132.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5398..5502
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5576..6319
                     /locus_tag="ACHBBW_002495"
     CDS             5576..6319
                     /locus_tag="ACHBBW_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154133.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6334..7326
                     /locus_tag="ACHBBW_002496"
     CDS             6334..7326
                     /locus_tag="ACHBBW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9154134.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7338..8075
                     /locus_tag="ACHBBW_002497"
     CDS             7338..8075
                     /locus_tag="ACHBBW_002497"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154135.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8065..8787
                     /locus_tag="ACHBBW_002498"
     CDS             8065..8787
                     /locus_tag="ACHBBW_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9154136.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8871..>9290)
                     /locus_tag="ACHBBW_002499"
     CDS             complement(8871..>9290)
                     /locus_tag="ACHBBW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9154137.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3042 a         1603 c         1965 g         2680 t
ORIGIN      
        1 aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag tggttcaaat
       61 gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc cagatacttc
      121 actttattca atgtataaga tagaatagaa cagctaagta atgtatacag aaaatgtgtg
      181 agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa aagacttttg
      241 ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt tccgcctgtg
      301 cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa
      361 ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa
      421 aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga
      481 aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga aggagcgaat
      541 ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc cagcttagat
      601 ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat gattccgcac
      661 aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga aaattttcag
      721 caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa aaaaaattat
      781 ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc agcagactta
      841 ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt tttggctcaa
      901 gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca atttatcttt
      961 ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt tttgatgaag
     1021 aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct ttatggagtg
     1081 aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat
     1141 gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag tataatattc
     1201 gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct
     1261 ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt
     1321 cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt
     1381 tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt ttttttgttc
     1441 gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat gtttatactt
     1501 aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa gtgaaagaaa
     1561 aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt ctttgctgat
     1621 tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa aaatggcaga
     1681 agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct tattaggttt
     1741 acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg ttatttacgc
     1801 tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc cgtctattga
     1861 agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag tagagcttcc
     1921 catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa tcatcggtac
     1981 ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt tgttaggtat
     2041 tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct tgcttgcaat
     2101 tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt ccatcattac
     2161 tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg ccttgtttaa
     2221 aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat
     2281 gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac cgaattttgg
     2341 caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct atcctgagtt
     2401 cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt
     2461 gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat tcgatatgac
     2521 attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga gatcttttgc
     2581 gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc agatcaaagc
     2641 gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc aagaagcata
     2701 tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa
     2761 taatggagat gtcaatattg tggatgccta ttccacagat agccagctca aggagtacga
     2821 tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg caccgctgat
     2881 gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata aattaagcgg
     2941 aaaaataaca gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa
     3001 gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg aggaagatca
     3061 gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc
     3121 gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg tccttccggt
     3181 agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc agcaggagac
     3241 atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg gtggaatatg
     3301 ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca aaatattgaa
     3361 gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt agatgaactt
     3421 cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag agagttgtct
     3481 ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc agatgtgatt
     3541 ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt acaagaattg
     3601 gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca caatatggaa
     3661 gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat tatccaatgt
     3721 gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca tttctttgat
     3781 gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg atatttctta
     3841 aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat gaaagaagta
     3901 tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag aagtattggg
     3961 tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac
     4021 atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt atggattgaa
     4081 agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga cttgtcctaa
     4141 ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa
     4201 acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag aacttcaaaa
     4261 attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat tagcagaagc
     4321 tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta ttgaagtcaa
     4381 cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac ctacgatctt
     4441 accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat tgcctgtact
     4501 gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag ctacgattgc
     4561 caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat taaaagaatt
     4621 tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca ttcagtttgc
     4681 ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt tagtcggaaa
     4741 agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc cgaatacaga
     4801 atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg cagtcaatga
     4861 ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt caaggaaaac
     4921 cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc ggataacgga
     4981 tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg ccagtcctaa
     5041 acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag tggtagagat
     5101 ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt
     5161 gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc aagcagacga
     5221 attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag agttggatag
     5281 ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga aataatcagc
     5341 atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa aaatgaatac
     5401 ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga cccattgaac
     5461 ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg gttactatgt
     5521 cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta acaaaatgaa
     5581 gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc agattgttgt
     5641 catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat tttctgcatt
     5701 agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg aagcagtgct
     5761 tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg attattggaa
     5821 gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc caatcatggt
     5881 gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag tgatccttgt
     5941 tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga ggaaagtatc
     6001 aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga tttatcgaat
     6061 atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg ctgctactta
     6121 ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct tagggtatta
     6181 catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga tcatttgtgt
     6241 cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg
     6301 tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct tttattaccg
     6361 ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa acaagaaata
     6421 aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa tcatactggc
     6481 atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt ccaaatcatc
     6541 gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc acaatttggt
     6601 gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat accagtcaaa
     6661 gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc agacagtaac
     6721 atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa
     6781 gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa actgacaatg
     6841 gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat ccaatggtac
     6901 tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa ctatatccca
     6961 ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac gaatgatcaa
     7021 ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa aaaaggctat
     7081 caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa
     7141 aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta tacggatgat
     7201 cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc ttttatgcag
     7261 gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa tcagtttata
     7321 aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc attgaaaacc
     7381 tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca tcgattgtcg
     7441 cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc cagcctatga
     7501 cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca gcatttagtt
     7561 atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac gttacgttat
     7621 atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat ctggatatct
     7681 ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg atgaagcagc
     7741 gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt gatgaaccat
     7801 ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg aagctgccta
     7861 aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca atttatttat
     7921 cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag attcaagtgc
     7981 ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag caaacgattt
     8041 atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt agatgggagt
     8101 cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa ttgtgattcg
     8161 ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc cttaagttac
     8221 ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc ttttttgaaa
     8281 aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt accgatgact
     8341 acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga gaaacctgtt
     8401 gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc ttaccctaat
     8461 cactatttga tccattggta ttcttctttg gactatcaac aagactatat tgatttaggc
     8521 tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca actagctaaa
     8581 gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc agagtgggct
     8641 ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca gttaaagaca
     8701 atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta tcaaaacttg
     8761 gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt gtcgcacctc
     8821 tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc ttagtaccaa
     8881 ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga agtaacatat
     8941 tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta agaagaatct
     9001 aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct tgattctttt
     9061 tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt ttctgttaca
     9121 gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg aaccgtttct
     9181 tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
     9241 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DAYYKC010000103         9264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000103 DAYYKC010000000
VERSION     DAYYKC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.104"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBBW_002500"
     CDS             <1..435
                     /locus_tag="ACHBBW_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9154138.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHBBW_002501"
     tRNA            complement(759..842)
                     /locus_tag="ACHBBW_002501"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHBBW_002502"
     CDS             1370..1870
                     /locus_tag="ACHBBW_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9154139.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHBBW_002503"
     CDS             complement(2060..2404)
                     /locus_tag="ACHBBW_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9154140.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHBBW_002504"
     CDS             complement(2558..3478)
                     /locus_tag="ACHBBW_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9154141.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHBBW_002505"
     CDS             3665..4156
                     /locus_tag="ACHBBW_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9154142.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHBBW_002506"
     CDS             complement(4180..6555)
                     /locus_tag="ACHBBW_002506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9154143.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHBBW_002507"
     CDS             6881..7507
                     /locus_tag="ACHBBW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9154144.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHBBW_002508"
     CDS             7530..8234
                     /locus_tag="ACHBBW_002508"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9154145.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHBBW_002509"
     CDS             8426..9259
                     /locus_tag="ACHBBW_002509"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9154146.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2823 a         1785 c         1650 g         3006 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaa
//
LOCUS       DAYYKC010000104         9090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000104 DAYYKC010000000
VERSION     DAYYKC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.105"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            431..1234
                     /locus_tag="ACHBBW_002510"
     CDS             431..1234
                     /locus_tag="ACHBBW_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9154147.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1819..2274
                     /locus_tag="ACHBBW_002511"
     CDS             1819..2274
                     /locus_tag="ACHBBW_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154148.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2393..3451
                     /locus_tag="ACHBBW_002512"
     CDS             2393..3451
                     /locus_tag="ACHBBW_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM9154149.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3493..5442
                     /locus_tag="ACHBBW_002513"
     CDS             3493..5442
                     /locus_tag="ACHBBW_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154150.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5445..9086
                     /locus_tag="ACHBBW_002514"
     CDS             5445..9086
                     /locus_tag="ACHBBW_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFM9154151.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3272 a         1308 c         1896 g         2614 t
ORIGIN      
        1 tttttaagat tttgaaataa gagaggagta atcctccttt attaaatatc tgttaatata
       61 tctgttaaat atctgttaat atatctgtta ggggaggctt aatctcttgg gagagtaagt
      121 attaaagggg tataaaggta acacttttgg acagtaaagg taacaaaagt ggacagtaag
      181 ggtaacagtt ttggacagca aaggtaacaa aagtggacat ttgaggtatc acactaggat
      241 aagacgttac cttcggtttt cttgatttgg aaggcgaaaa aatagaaaat tagattagta
      301 acaaatcttt gttgactgtt acctttagtt gtgatttaat ttaggtaaca agcagaaaaa
      361 attgtttcct ttaattagta ccagattaac atgattaaag gagcgcaaaa agaagattgg
      421 agagtaatca atgtctcaag aagttaccga aaagattatt gatagtgatg tagatttagt
      481 atcagaacaa acaaatgcaa ttgtgcgtta taacaatggt ctgaatacaa cagctttgcg
      541 aggatttaag cctgttgaaa tggatgtgtt ttgggctatt ttatcaaaaa tgaaacgtca
      601 aggaacagca gaagtaactt ttgatttcaa cctctttcgt tatttggtca aatacgacag
      661 aagagaaaag gacagttttt attctgtgtt aaaagatttg agtgacaaac taggtaattt
      721 aacctaccgt tttgaagatt cagacgtcta tgaacagctg tggctttttc aaaaattccg
      781 gatagataaa cagaaggaaa cagttacaat taaggcaaat gaggaatttg aatttatctt
      841 aaactcaatt ggcaaaaact ttactcgttt tgaacttgat aattttacaa aactaagaag
      901 tagctacaca aaagaattgt atcgtcatct catgcaatac agaaataaag agtggcgaaa
      961 tggttattgg gtagtcaaag ttgaagattt tagaagagca ttaagtatac cttcaagtta
     1021 tagaatgaca aatattgata aaagaatact agagcaggcg aaagaagaat ttttagctcc
     1081 tgatgaaaac ggaatacagg tatttgaaac ttttgattat gaaaaaatct atgcaaagaa
     1141 aggaagaaga gttgatcggc tcagattcac ttttagtgag ccagaatcaa acttgccaac
     1201 catttcaatg cataattggt tagaagaaga ttgagaagaa gcaggttgta attttaatac
     1261 aacctgcttc tttaaaaaag tatgggtaac acttttggac aataaatgca agaagttttt
     1321 ttgtaagcaa gtttcagtct aaataatatt tgaaagaatg gctaatctat acaaatttta
     1381 acaactatgg gtaacacttt tggacaagtc aagaaaaaaa acaatttttc ttaatttgtt
     1441 gatcgactaa aggtaacact tttggacaac acagaataaa aagtccggtg atatcagttt
     1501 tggtaggaaa catcttaata tctttgtgag attgaaggta acatttttgg acaagtcaac
     1561 tatttgttaa aagttaatat agagtaatta gataagtttt ttcttttcgt ctgataaatg
     1621 gataatatta gttattttat tgctaattag tctttcatga tgactgaaat agctaaaata
     1681 aatatctcgt tggataattg ctgatacttt aagggatatc taaatttgct gattgattta
     1741 tttatgttat tatgatatta taataacata aatgtgttac gcccgaacct cattttaaaa
     1801 aaatgaaggg gtgagacaat gaatgctatt aattggcatg aatttgttaa ctatatggag
     1861 agcttactag atgatctaaa aagttttagt attgctgatg cagtaaagca tgggttacca
     1921 gttagtacaa cgatgaaatt acgaggattg aaaattccag atgaaattat taagctgaga
     1981 actaaaacaa ttattgaaac cgcaaaagtc gttgacaata tgaaccatga actaaaaggc
     2041 aagaaaacaa cttacgagaa agattttgaa cgttttttaa aatctgttat tggaaaatat
     2101 ctaggtaaaa aatctttcag tgaattgaga gatgaagaga ttgtacctac tccaatggtt
     2161 agcaaaatcc gaaaaaacaa agccgagggg aattactatc ttggtacaat agcaaaaatc
     2221 attgattatt tggaaaataa agagcaaaag aaaggaacga agaaaggaag ataatttttg
     2281 tgttaaatat taatggggaa gaatataccg aaatgattca aaatcccgaa cctagaatta
     2341 ccgaaaaaac gtctgattgt ttgctctttt atgaaagggg gcagacaaac taatggaaaa
     2401 aaacgtcaat tcaacatcta ccaaagatca atacagtatt attggagggt tgcttggtga
     2461 taaggataag agaacacgtc acggattacg cattatgctt gcaacttttc taggagtgct
     2521 ttttttatta acaggcttgg gtagctacat ttatcaatta ggaaactata attattatct
     2581 cgtgaacaat acaacaccta taggctcttc attggagttt gctcgatcag gagcagagat
     2641 cactatttca gatgtgtgga cagatcaaaa cagagatgta acagtagtaa aactcggtta
     2701 ctcgcaaaac gctagaaagt tgttatctac gcaaggaaaa aactacaact tatatatggc
     2761 aacgaaagaa aaagatcgtc ctaaaaagct tgaaatgagc tatgggatgt taagtactga
     2821 tggggatgct ttcttattcc taaaaggtaa attagatgaa agagcttatc aagtttttat
     2881 tgcaaaccaa gtatcactaa tttctggtga tgatacagct gacgatgata ccaaagtcaa
     2941 tgtgaatgaa gcagattcag ttacaaaagc actttctcaa tattctatga atgatgttaa
     3001 taatgaagga gtaatttctt ttgggaaaaa agcaaaacct gttaatactc ctgataatat
     3061 caatttcaga ataaatccat attcagattc aactaatgtc tatcaaggaa gctttcttac
     3121 caaagatggc gatattgatt attcaaaagt tgtagcagtt acaagtaaga ataatgttgt
     3181 aagtaagtta gaagatcagt taaatgcttc aaatactaac atcaagaaaa ttgaagctac
     3241 aaaagctgag tatctggaac gtttacaaca aaatcctgac gataaagatt cgcaagaaag
     3301 tattcgtcaa ctagacagtc gaatggagac cgaaattaaa gcaatcaatg atacacaaaa
     3361 attgattgat caatacaaaa atgctgactt taacaaagat tcttttggag acatgaagac
     3421 gacatttaag tacctaactc tgactgacta gaagtaaatt attcttattt tagaggaagt
     3481 gaacacaaac agatggaaaa tagtaccgag ttttttaaga atattcaaac tgaatttaat
     3541 catgctctca caccaatgaa accaccgaaa cataagttag tgggacgtga tgacgaactc
     3601 catgagttgt cactgatctt gaatagaccg gaaacaccgc aagctatctt gttaggtgaa
     3661 gccggaacag gtaaaacagc acttgcaagt gaatgggctg aaagacagcg acaagatgga
     3721 aagaaagtac aagtatacaa attatcaatc ggacaattgc gtgttggggg agatgatgtc
     3781 ctccaaacac gtttagaaac attaattcca agattgaaag aatatgagcg aattttaaaa
     3841 tctgaggaca aagaaaatga atttgtcctc ttcattgacg agtttcataa agttgttagc
     3901 tcttttggta gtggcaataa aattgggggt gattcgctaa aagaaccgtt agcaaatgaa
     3961 tcacttcaat tgattaccgc tactacaaga aaagaatatg aaaactatat caaaagagat
     4021 gatgccttag ggagacggtt acaaccgtta gaattgaatg aactaaaaag cgatgttgtc
     4081 gtcgatattt tgagacaatg gctaaataca tcctctgtta aagatgatcc tcaggcttct
     4141 tttaaattat cgcaaaggat tcctgatgaa ttacttcaag aaatcgtaca agcaaacaag
     4201 ctttatcgtg aggatttgtc cgaaccggct aaatcaattg ccgtaatgga ggatttgatt
     4261 gcaaaagatc gagtagacgg aaatgcttat aatcgagaaa gtctattgca aatatttggt
     4321 aagtataata ttcagctaga ttctgatttt gatatggaaa aacttcaaga ggaattaaag
     4381 tccataaaag ggcaaccgtt agcagtttat actataacaa tgttgtttaa acgtatgaag
     4441 cttggatttg atcgttttaa aggtcgaaag caaccaaaag caactgctct gtttacagga
     4501 tcaacagggg tcggtaaaac tgcaatggct aaagccgtgg caagaggact ttatggcgat
     4561 gaaaataaag ttctcttagt taatatgccg gactacaaaa caaaagatga tgcaatgcgt
     4621 tttagacgac tcataggtac tcatgtaagg catgatcctt ctgcgattat tgtgtgtgat
     4681 gaatatgaga aagctggtgt agaaacactc gatgcctttt tatcaatttt agatgaaggt
     4741 aggataagtt ttactgaaag aggatgggat gatcgagaag ttcacgacac tgtttcattg
     4801 tcaaatacaa ttttatttgc gacaacaaat gcaggtgcgg agatgttcga ttcgttaaac
     4861 agatatagct cactatctcg tgttacaaac aaggaaaatt ctaatgaata tagcactgaa
     4921 ctaaaaagag aataccgaaa agtcgcatca gggatcaaac aagccttgat tggtgaaggt
     4981 ctaaagcctg aagttattaa ccgatttgga cgaatagttc cattttgggc tttatccgaa
     5041 caaactcttc tggaaattgc ttataatgaa atgttgaaga gtgctgaatt agtacgaaaa
     5101 catttaaata tagatttacg tcttccacct aggaaagaaa gagtatatgg tcaaaatttt
     5161 tatgcagacg atctctccat gcacttagtt tttgacatgg cttcacaaga ggattctaaa
     5221 tcagggggtg caaggaatat cttgcattta gttgaatctg agtttgaaga agcgattact
     5281 gaagcaataa ttacttatcc aaacgcaaaa atattcgatg ttaagttgag tgaagccgga
     5341 gcgtttgaag gaaaaggtga ttctaatgct gaaattattg caaagccttc cgttgctgaa
     5401 agtaaagaaa acaagattgt attttcgatt ggaggggcgt agctatgaaa ttcaagaaat
     5461 atttactaca aaaccttaga ataaaacgat gggtcgtaag aggtctagtt attggaggag
     5521 tattagcagt tactttaggc gctccttttg gaatgaatga tccaactcct gacaatcaag
     5581 atagcaaaca agaaacagtt ataaataaga acgttgcata tgcggaaagt aaaccggcag
     5641 aacaaggaat gtggacacta tatgctcgta tcctaatggg agataaagaa gttaatgata
     5701 gtgccgctaa aactggacaa atgaatttgt taggacaagg tggtatcagt gccgatttcc
     5761 cttattctga catttctaaa caaggtaatt cgattgcagg gggtaatgca ggtactcaac
     5821 tagcatctac tttagcaact tatagttact acggatatat tgaaacagtc tcaggaaatg
     5881 ctattgccag tcaagcatca tccattctag gaagtattgg taggacgatt ggtagtttta
     5941 tcggatcaat tagtttaatc ctttctaata ttgctgaatc cataaatgaa ctccttggaa
     6001 aatttttcat gagtatgaat atattttctc ttatggggtg gggagaaggt ggatcggatg
     6061 tatctaatcc ggtatctaaa gcattaacta gcttgcttaa caatttggga ttaaataatg
     6121 aattatttaa agcgatagca agcttatcta tgctcttttt cacagcaata ctaggttatc
     6181 aaggaatgaa attattgtcg aaagatggtg ttaactcgaa gggattatca ccagtacttg
     6241 aaaaatggat tgttcgtatt tttgtattgt ttatattctt accagtggca ggatcgctat
     6301 tgggagttta tatcaacggc atccagtcag caaaaaaaga tggagatata ccaacaaatg
     6361 ttgtttctga ctacatcatt aacacaagac tttgggcttc tactcaaaat cttgctccat
     6421 catctgatac tggtggtgat gtacctgacg gaaagcctac atccgaaatc aagttcgttg
     6481 atacagatta tgaaccatct aaaaaatctg caatgataag agcgatcaat actagcagtt
     6541 atgccagaca aaataatgat ctagattcta attcagtcgg tatggctcta ttaaaagaat
     6601 ggtcatcgaa tgatactttc aatattaata cttatgccgg tgatgttaat gtcgccgaaa
     6661 atgatgctta tatgggaaga gcgcctgctg tttggacagg ctataattta ggggatggaa
     6721 aagaaggaag tgcaattaag aaaccaacca atttaaatgg atatatttgg tcagcaaatc
     6781 aaaatgtgac agacgacaat cgcaagcctg atggaaaagg aacagcgcat aactttgatg
     6841 ctactcaacc ttggggcgtt gctaacaatg caacttttag cacacagtcg gtagctttat
     6901 tgctccaatc ggcacttgat acatcgggag ctaagtttta tgcgtataat atcgctcctt
     6961 caggaattca aggcagtgct aagaatatgt caacagtaaa aacagaatgg cgtacagcat
     7021 ccttagttgg tcatggagct atgggtaagt ttggctcgtg gctagcgatg aacgctgaat
     7081 caatcgtttt agcagttctt tacttagcat gtgcttgggc gatgttcaat gtgaatatcg
     7141 tgaaagcatt taaacgtgaa tttattcgga tttgggaagt agttctaaca ggatcacttg
     7201 catcaggagc aggtgtgatg gcaatttcat taggtgttgt tacaacatct ctgatcggac
     7261 tacaattacc tatctttctg attactttta ttcgtgcatt tggtgatact gctgtgggat
     7321 tcattcctaa tacattagtt ggcgatggcg tagcaaatat tgttcgatct gctatgattt
     7381 taggtgtagg atatctttta gtctttaaaa aaggcgaaaa agtaaataat ttgattaata
     7441 tgcttattgg cttaccactt gaagtagcgt ttgggtttga tgaacgtgct aagttattat
     7501 ttggtaatag aaatgatgct gtgcgagttg tggacggcgt ttctaaagca atcaagaaaa
     7561 gccaaggagc taaaaatccc tattatgatg gtggcaacag ctcagtaaaa gatatggttg
     7621 caggaggtct tggagcagtt ggagcgcaaa aaatggctga acgtttgaac aatatgggag
     7681 atagcaatgc tgatgctaag tccattgctg ataaagtaat tcctgaaaca ccagaaggaa
     7741 tgtcattcaa cggtggcgga gttggttcca caatcaataa tacctctttc aaagatttag
     7801 ttcgtcaaga tcaactacgt aataactccc aacaatatat tgatcattta ggacgtatgg
     7861 ttgatgaaaa cggaaacttt gtcgatgaaa atggtgaaat attaaaagat agtaaatacg
     7921 gtaaagatgc taaaggcaac tttattgacg aaaaagggcg tttggtcgat gaagatggaa
     7981 accttataga tactaatggt aaaccattgg aagatagtga agatggtaat attggtatgg
     8041 atagcgaagg acgtttaatt gatgaaaaag gtcgcttagt cgatgaagat ggaaatcttg
     8101 ttgacgaaaa tggacgcagg ttgctaaatg gtgattttgg tcatgatagt aacggacgtt
     8161 tagttgacga aaaaggtcgt ttagttgatg aagatggaaa tcttgttgat gagtttggta
     8221 ataaacttgg taaacacagc gatggatata atgtgaatgg agatttagtc gatgaggatg
     8281 ggaatatcat tgagacgaga gatgcgcttg tggcagatcg aaatagagaa ctcgaacaac
     8341 aacggcatac gccaattgct gttgcaattc ctgagaatga agaagacgag attactttag
     8401 atcaaactca tcaagaactg aggaatgaaa tgagtggtga ggacgtgtca gacagagtgc
     8461 atcgggaatt aggagatgga gcatctggtg aaagtcagtc aagagagctt aacagcaact
     8521 ttgataatag tgctattcgg aatgacacag tacttaaaga cagagaatcg ctaacaaaag
     8581 aaacggataa agaacaatca gatgtgaaga aaaagttcat tcctactcct aatgttaatc
     8641 aaacagataa atatgaaaca aatattggat caggaatgtc gaaagacaat caatcaatta
     8701 gagaacctga actgacaaat aaaaataata gaacaaataa ttctaaatct acttcagata
     8761 attcagggaa atctaatcaa tcaaccaaga aaacatcaac agctaaatca gccgttgaat
     8821 atctatcaaa aactggtaaa aatgtttaca cctcagcaga taaagcttta gcaaaagctc
     8881 caaaaggtag tctagcaggg ataacctatg aagctaaaaa cgaagctatt aaaataggta
     8941 aaggaacggc taaggttgcc aaggctactg ctacaggagc atccaaaact ggtaaagtag
     9001 tgaagaaatc taccatgtca gtcaaggact atattgtaaa atacggtaat caaaaatcaa
     9061 ctgaaaataa gtctgaagac gagtaaaaag
//
LOCUS       DAYYKC010000105         9072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000105 DAYYKC010000000
VERSION     DAYYKC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.106"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..539
                     /locus_tag="ACHBBW_002515"
     CDS             96..539
                     /locus_tag="ACHBBW_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9154152.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(545..1729)
                     /locus_tag="ACHBBW_002516"
     CDS             complement(545..1729)
                     /locus_tag="ACHBBW_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9154153.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1997..2695
                     /locus_tag="ACHBBW_002517"
     CDS             1997..2695
                     /locus_tag="ACHBBW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9154154.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2702..3379
                     /gene="nth"
                     /locus_tag="ACHBBW_002518"
                     /pseudo
     CDS             2702..3379
                     /gene="nth"
                     /locus_tag="ACHBBW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3505..4038)
                     /locus_tag="ACHBBW_002519"
     CDS             complement(3505..4038)
                     /locus_tag="ACHBBW_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154155.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4335..6716)
                     /locus_tag="ACHBBW_002520"
     CDS             complement(4335..6716)
                     /locus_tag="ACHBBW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9154156.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6706..7353)
                     /gene="recU"
                     /locus_tag="ACHBBW_002521"
     CDS             complement(6706..7353)
                     /gene="recU"
                     /locus_tag="ACHBBW_002521"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9154157.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7417..7956
                     /locus_tag="ACHBBW_002522"
     CDS             7417..7956
                     /locus_tag="ACHBBW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9154158.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8046..8465
                     /gene="gpsB"
                     /locus_tag="ACHBBW_002523"
     CDS             8046..8465
                     /gene="gpsB"
                     /locus_tag="ACHBBW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9154159.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8487..8854
                     /gene="rnpB"
                     /locus_tag="ACHBBW_002524"
     ncRNA           8487..8854
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBBW_002524"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         3023 a         1589 c         1845 g         2615 t
ORIGIN      
        1 cttggtttca gattgggttt tcctacaaaa tatttttatc tttgttgaat aggaaacaaa
       61 aaaacaatat aatgaaagaa aaagaaggtg tagaaatgga taaaagacaa ctgctaatag
      121 aagaatatct ggatttagct aatgagatta ttcatcgtaa taaaccagaa atggaaaaag
      181 aatttgcagg atttactcta aatgaactag aagtgattga acatatcggg aaaatccctg
      241 atcctaacgt gacaaaatta gcgaatgcta gttacatgac tagaggagcc atcagtaagc
      301 taacaaaaaa actgatagct agagggatca taaacactta tcaaagcgaa gagaataaaa
      361 aagaaatcta ttttcaattg acgccaatcg gacaaaaaat aaggaataga cacgagcaac
      421 tccatcaaaa atgggtacag cgagatgcta ctgtatttga aaatatgtca aaagaagaat
      481 ttgacacagt atttcgtttt atcggtcgat acagagaatt tatacgaagt agcaaatgaa
      541 gctactattt tttattgtag tgataatagg caaaaatcat aagcaaaaat ccgaataccg
      601 tcggaagaaa tggtgctgca gcataaagct gtcctgcgat tagcgggccg atgacgcgcg
      661 atagagattg tatactttgg gtaccgccca ttacaagacc ttgctggtta tctgagacag
      721 aattggatag ttggccattg aatacaggag taaaaataga atcaccgaaa ccataacaga
      781 taatcccaaa taaaaacaaa atcggcagta gaagaattcc agataatgtg atgaatagat
      841 aggcgaggat ttcactgaac atccctattc ttgtgatctg ctgttctgaa aattttttga
      901 gcaatcgagg cataatgaac aattgcgaga cagtatccaa gattccaatc aaagagaagc
      961 ttaaaccaac caacccagcc ttccactgaa aggcgtcaat cgaaaattga gaaaagatag
     1021 attgaagaga gccagctgcc aaccaaacag caaaacctgc tgtcaataaa ggaataacgg
     1081 atgaaatctt gaataattgt tgtaattggt gaaaaggtct tacatgggag aaacttaaat
     1141 ctactgaacg tttttttatt ggaaggcttt ctagcataaa tgtgtagcca taaacggcat
     1201 ttaagaaagt aaagagtgct ccaataaata taggaacaga attccccaac tcagctaata
     1261 gaccccctat aataggaccc agcgttgttc ctataccgac taaagcaccc atccatccaa
     1321 aaactttggt tctttcatta ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga
     1381 tttctcctgc cgtcaatcct tcaatgatac gaccaaggaa caacatccat atcgaatttc
     1441 ctaaaccaaa aagcaaatat ccgatacttg atcctaataa actgataata aggatcggat
     1501 gacgcccaaa atgatcactt aagctaccga gaatgggggc agataagaag gcagctagag
     1561 cataaacaga catgagcaaa gtgatataga aagcttgctg atgtatggag tgagaataag
     1621 gcagcgttaa aaacggaagt acaggactga cgatagtaaa tcctaatcca gtgaataaga
     1681 cagagacgta tccaaaaact ttagaatgtt tatttgtgaa actgtacatg taaaaactcc
     1741 tttatgcaat aatgttaact tggaaacaaa aatagtgtat agtaattttg tttccttgtc
     1801 aacattctaa ggaacagcga aatctactta atttatttat tccgaaaagt gaagatacgc
     1861 atcaaaaaca tgaacaaaca aaaatcaagt agacgacatt gtaaatctga ccgatcagtg
     1921 aagtaaatag aaaagaaacc tgttcttgct tcccatgttt tttttacgta tactgtgatt
     1981 gagcggagga gaaatcatgt tgaatttaga aaattactta agcgctggac aaacagtcat
     2041 atctaatctt ttacttaaaa attatcatag gatcggtcta caatctgaag aatttttatt
     2101 tgttctacag ttgcatatgg cacagcttga aggagatact ttccctgatt tgcagatgat
     2161 tgctatggat atgggaatca aacaagatca gatttttcaa atactggatc gtctcgtaac
     2221 aaatggattt atcaagatag aaacgacgat tgtcaacggt aaaaaagcag atcgatataa
     2281 tctttacccc atctatgatc tattaggtga gtatttaaaa acacaagaaa aaaaatacga
     2341 agaaaaaaca caagaaaaag aaatccagtc agtgtatcaa ttatttgaac aagaatttgg
     2401 acgcccgctt tcttcgatcg agttccagcg gatcggacaa tggctagaag aagaccatta
     2461 tcagccggaa attttgaagc tagcactgcg ggaagccgta ttaaatcagg cttatagttt
     2521 taattacgtt gaccgcattt tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca
     2581 agtggaagaa gaccaaaaac gaagaaaaca gcagcttctt caaagagaag tagaacagtc
     2641 agctcaacaa gaagaattgc cgaaagtctc actgaaaaat tggttggagg agtagcggaa
     2701 tatgttaagc aaacaaaaaa caatggaagc tctagaaacg atgtatggaa tgtttcccga
     2761 agcacatgga gaactcaagc acaataatcc atttgagtta ttgattgcag tgatcttaag
     2821 tgcccaggcg acagatgtat cagtgaataa agcaacgcca gatttgtttg cctcttttcc
     2881 gacacccgat gcgttagcag aagcttcaat cgatgaaatc attcttaaaa taaaaacgat
     2941 tggtttgtat cgaaataaag caaaaaacat caaagcgtgt gcacagcagc tgattgaacg
     3001 tttcgatggg caagtgccaa cgtcaagaga agaattgatg tctcttcctg gtgtaggcag
     3061 aaagacagca aatgtcgtat tgggggatgc attcgggata ccagcaatcg cagtagatac
     3121 ccacgtggaa cgtgtctcaa aacgcctgag gatctgcaaa ctagatgcta cggtcatgga
     3181 agtagaagaa acactgatgc ggaaagtacc acaagaatta tgggtaaaga cacatcatac
     3241 actgattttt tttggttgat atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc
     3301 tttgctatca atatgtcaag atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa
     3361 aaagaaacca cgcctgtaga cgtggtttct ttaattgcaa ataaatgtat gaattatctt
     3421 gttattatgg agaaaagctg tttcgtttat cttttttgca tgaaaaagta aatgtacata
     3481 ctcagtagct aggatggtaa cttattacaa tttattgcga taaaaataaa agttgttatt
     3541 tttattggga aaatacccga tgtagaaaat aatgccttcc aatataccta taaaataagt
     3601 ccagtattcc aaaggattat ataaataagt gacgtatcta tctaaaggtg taataaaaat
     3661 gataagtgat aataaaaaaa taccatttat aactaaacat aaactaatgg atttgggcgg
     3721 tttcaggctc atcagaaaaa tatagattct ggtgaacaac aaccataaaa aagtgagaat
     3781 cattgcatga gagcttcttt ccataaattt tcctttaaga gtagtgctta aaagtacaga
     3841 aaaaataata aatgcaagga taggagcaat taaagtaaat ttatttaata ataaacaaaa
     3901 tttttcagct tttttattct tggaataacg aggtattgtt ttcgataaaa aaagatggtg
     3961 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
     4021 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
     4081 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
     4141 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
     4201 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
     4261 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
     4321 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
     4381 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
     4441 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
     4501 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
     4561 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
     4621 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
     4681 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
     4741 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
     4801 tctgaagtga caggcgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
     4861 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
     4921 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
     4981 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     5041 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     5101 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     5161 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     5221 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     5281 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     5341 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     5401 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     5461 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     5521 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     5581 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     5641 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     5701 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     5761 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     5821 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     5881 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     5941 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     6001 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     6061 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     6121 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     6181 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     6241 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     6301 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     6361 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     6421 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     6481 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     6541 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     6601 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     6661 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     6721 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     6781 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     6841 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     6901 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     6961 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     7021 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     7081 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     7141 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     7201 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     7261 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     7321 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     7381 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     7441 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     7501 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     7561 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     7621 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     7681 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     7741 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     7801 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     7861 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     7921 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     7981 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     8041 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     8101 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     8161 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     8221 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     8281 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     8341 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     8401 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     8461 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     8521 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     8581 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     8641 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     8701 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     8761 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     8821 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     8881 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     8941 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     9001 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     9061 gtatggaaaa aa
//
LOCUS       DAYYKC010000106         9018 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000106 DAYYKC010000000
VERSION     DAYYKC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.107"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHBBW_002525"
     CDS             <1..1101
                     /locus_tag="ACHBBW_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9154160.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHBBW_002526"
     CDS             complement(1280..1615)
                     /locus_tag="ACHBBW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9154161.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHBBW_002527"
     CDS             complement(1599..1862)
                     /locus_tag="ACHBBW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9154162.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHBBW_002528"
     CDS             complement(2322..3125)
                     /locus_tag="ACHBBW_002528"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9154163.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHBBW_002529"
     CDS             complement(3103..4227)
                     /locus_tag="ACHBBW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154164.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHBBW_002530"
     CDS             complement(4243..5058)
                     /locus_tag="ACHBBW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9154165.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHBBW_002531"
     CDS             complement(5048..5920)
                     /locus_tag="ACHBBW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9154166.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHBBW_002532"
     CDS             complement(6005..7294)
                     /locus_tag="ACHBBW_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9154167.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHBBW_002533"
     CDS             complement(7438..7950)
                     /locus_tag="ACHBBW_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154168.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHBBW_002534"
     CDS             complement(8017..8160)
                     /locus_tag="ACHBBW_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9154169.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACHBBW_002535"
     CDS             complement(8643..>9018)
                     /locus_tag="ACHBBW_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154170.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAYYKC010000107         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000107 DAYYKC010000000
VERSION     DAYYKC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.108"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHBBW_002536"
     CDS             complement(13..1152)
                     /locus_tag="ACHBBW_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9154171.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBBW_002537"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBBW_002537"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9154172.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBBW_002538"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBBW_002538"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9154173.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBBW_002539"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBBW_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9154174.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBBW_002540"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBBW_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9154175.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHBBW_002541"
     CDS             complement(6166..7257)
                     /locus_tag="ACHBBW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9154176.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBBW_002542"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBBW_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9154177.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHBBW_002543"
     tRNA            8800..8871
                     /locus_tag="ACHBBW_002543"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYKC010000108         8914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000108 DAYYKC010000000
VERSION     DAYYKC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.109"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..903)
                     /locus_tag="ACHBBW_002544"
     CDS             complement(406..903)
                     /locus_tag="ACHBBW_002544"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9154178.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(943..1752)
                     /locus_tag="ACHBBW_002545"
     CDS             complement(943..1752)
                     /locus_tag="ACHBBW_002545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9154179.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2086..2943
                     /locus_tag="ACHBBW_002546"
     CDS             2086..2943
                     /locus_tag="ACHBBW_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9154180.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2936..3838
                     /locus_tag="ACHBBW_002547"
     CDS             2936..3838
                     /locus_tag="ACHBBW_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9154181.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3871..4221
                     /locus_tag="ACHBBW_002548"
     CDS             3871..4221
                     /locus_tag="ACHBBW_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9154182.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4270..5193
                     /locus_tag="ACHBBW_002549"
     CDS             4270..5193
                     /locus_tag="ACHBBW_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9154183.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5271..6164
                     /locus_tag="ACHBBW_002550"
     CDS             5271..6164
                     /locus_tag="ACHBBW_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9154184.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6202..6765
                     /gene="amaP"
                     /locus_tag="ACHBBW_002551"
     CDS             6202..6765
                     /gene="amaP"
                     /locus_tag="ACHBBW_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9154185.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6779..6967
                     /locus_tag="ACHBBW_002552"
     CDS             6779..6967
                     /locus_tag="ACHBBW_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9154186.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6980..7882
                     /locus_tag="ACHBBW_002553"
     CDS             6980..7882
                     /locus_tag="ACHBBW_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9154187.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7914..8156
                     /locus_tag="ACHBBW_002554"
     CDS             7914..8156
                     /locus_tag="ACHBBW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9154188.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8412..8783)
                     /locus_tag="ACHBBW_002555"
     CDS             complement(8412..8783)
                     /locus_tag="ACHBBW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154189.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3107 a         1540 c         1726 g         2541 t
ORIGIN      
        1 gttcgagtcc tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa
       61 gaacagccaa gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat
      121 gcaagctttt gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat
      181 tagcttaaca aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag
      241 ggtcagctga ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt
      301 cataaaaaag ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg
      361 ctttttttat tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat
      421 agagacgttg aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac
      481 taacattgga tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt
      541 agagagtaca cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt
      601 gctttcaaat aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata
      661 ctttttaatc actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc
      721 tcgttcttct tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc
      781 attattttgg cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc
      841 tggaatataa aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa
      901 cattttcttt cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa
      961 aatataagca acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac
     1021 ttgtcgctgt gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc
     1081 gttttcttga tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag
     1141 aaaaacaagt gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc
     1201 agagaatact gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc
     1261 ttcactctta tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt
     1321 taaaatttga tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt
     1381 gactagattt gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat
     1441 ggatattttc gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt
     1501 tgaacgtctg agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt
     1561 ggaagaaatc aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct
     1621 gttccttccg gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg
     1681 gatattttct tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc
     1741 tgctagtttc attttatctt cctactttct ttttacatat ctattttcct gttaccttaa
     1801 gattaaatga gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa
     1861 aaaacattct taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata
     1921 aaaagccatg aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt
     1981 aaaatattca atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta
     2041 gatgtagaac aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac
     2101 ggcgtagttc ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg
     2161 atcgcaactg cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac
     2221 gatggtctat cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt
     2281 aatgcaagta tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt
     2341 gaccatatca caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag
     2401 gattataaga aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa
     2461 ctatatgatg agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg
     2521 aaagctttgg aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg
     2581 gtgattgaca ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc
     2641 agtgaaaatg gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat
     2701 gaagattggg aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt
     2761 catccagcac caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg
     2821 atcgttcact ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc
     2881 aatttatact taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa
     2941 taaaaaagaa atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc
     3001 attatttgtc tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat
     3061 tgacgatgat atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg
     3121 aatgaactca gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat
     3181 cagcgatcgt attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg
     3241 cacagaagtt gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc
     3301 taaattatgg cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt
     3361 ccatcaacga ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg
     3421 tttgatgctg atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga
     3481 tttcatcgaa cataatccag aaaaattacg attccatgac caagatgcat taaatgctat
     3541 cttacatgac cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc
     3601 aaaagcaaaa aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa
     3661 tccttacatt atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc
     3721 aacgaagaaa tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt
     3781 ccctaaatat ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata
     3841 gaagaccaca actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta
     3901 gagacgttaa ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat
     3961 tccgatccat ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt
     4021 ccttttgttt cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc
     4081 tatacatcta aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa
     4141 cagcttgaca gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta
     4201 aagaactgct tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga
     4261 tactactttg tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg
     4321 ctcgtacttg atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt
     4381 cctgatgata ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt
     4441 tatgagacga aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat
     4501 aatcgtatcg aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt
     4561 acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat
     4621 ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg
     4681 aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca
     4741 acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat
     4801 cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc
     4861 aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa
     4921 aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg
     4981 atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc
     5041 gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt
     5101 tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt
     5161 taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac
     5221 aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac
     5281 caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg
     5341 atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg
     5401 aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg
     5461 gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc
     5521 caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga
     5581 ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag
     5641 ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt
     5701 gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca
     5761 tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg
     5821 taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa
     5881 caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa
     5941 tccgtgtaaa tggtgtagca cctggtccaa tttggacacc attacaacta gacgaaggac
     6001 aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc
     6061 caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag
     6121 cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca
     6181 cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca
     6241 ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct
     6301 tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta
     6361 ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt
     6421 tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat
     6481 aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca
     6541 ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg
     6601 aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa
     6661 gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt
     6721 gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat
     6781 gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt
     6841 agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc
     6901 gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa
     6961 atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa
     7021 ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa
     7081 cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt
     7141 tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg
     7201 aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga
     7261 agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac
     7321 aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga
     7381 acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat
     7441 cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa
     7501 tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg
     7561 taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa
     7621 aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga
     7681 agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga
     7741 tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc
     7801 gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa
     7861 tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat
     7921 ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata
     7981 aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg
     8041 gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat
     8101 tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag
     8161 acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca
     8221 aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga
     8281 acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg
     8341 cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca
     8401 gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac
     8461 atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac
     8521 tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa
     8581 taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga
     8641 caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct
     8701 agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga
     8761 aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt
     8821 aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat
     8881 ttttaaataa ataatcatta ttatcaaaat aatg
//
LOCUS       DAYYKC010000109         8713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000109 DAYYKC010000000
VERSION     DAYYKC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.110"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..234
                     /locus_tag="ACHBBW_002556"
     CDS             <1..234
                     /locus_tag="ACHBBW_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9154190.1"
                     /translation="FNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNS
                     SLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
     gene            662..1054
                     /locus_tag="ACHBBW_002557"
     CDS             662..1054
                     /locus_tag="ACHBBW_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154191.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(1227..1430)
                     /locus_tag="ACHBBW_002558"
                     /pseudo
     CDS             complement(1227..1430)
                     /locus_tag="ACHBBW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1426..1593
                     /locus_tag="ACHBBW_002559"
     CDS             1426..1593
                     /locus_tag="ACHBBW_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154192.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1636..2211)
                     /locus_tag="ACHBBW_002560"
     CDS             complement(1636..2211)
                     /locus_tag="ACHBBW_002560"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9154193.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(2366..2892)
                     /locus_tag="ACHBBW_002561"
                     /pseudo
     CDS             complement(2366..2892)
                     /locus_tag="ACHBBW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3349..4860
                     /gene="gadC"
                     /locus_tag="ACHBBW_002562"
     CDS             3349..4860
                     /gene="gadC"
                     /locus_tag="ACHBBW_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM9154194.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4873..6273
                     /locus_tag="ACHBBW_002563"
     CDS             4873..6273
                     /locus_tag="ACHBBW_002563"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM9154195.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(6438..7873)
                     /locus_tag="ACHBBW_002564"
                     /pseudo
     CDS             complement(6438..7873)
                     /locus_tag="ACHBBW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            8266..>8713
                     /locus_tag="ACHBBW_002565"
     CDS             8266..>8713
                     /locus_tag="ACHBBW_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM9154196.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2839 a         1402 c         1541 g         2931 t
ORIGIN      
        1 tttaatgaag aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt
       61 gaaaaaaata acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag
      121 tcaaaaaata gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta
      181 gaggataccg caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag
      241 ttttaattta acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat
      301 tatacaatct aaacaccgtt tccttttcta tttttgatga catcagtata tagctactcg
      361 aaagagtagt ctttttatta ttctcgctta ttattgatat gaaaatttcg atttgacaaa
      421 aggtaccgaa taattttgta atatgaatta ttatcaagaa gtaattatga tgataacata
      481 tggaaactag ttagtaataa taacaggtga ttaaactttt gaacgacttg taattctata
      541 ttttttcttt taagtaaaaa atataggtga ttttttttga cttcatattt ataataaaga
      601 cacgtaaatc aattattttt cattttactt taactaataa tgtttatttg gagggattat
      661 tatgttagat gatagattga cgtgttctaa ttgtggtaca ttattttact atgaggaagt
      721 tagaaatatt gtaaaacata gaaaaaaaga aatgcctata cgttgtccaa attgcaatga
      781 agttattaag cgaaagttgt ctcatggtta ttttgtcagc tacgaactat atgaagaaag
      841 taaaataaat ctgaaaaaaa aaccaaaata ctataaaaat ttgaaaactg gagctattat
      901 aactgaaaaa gaatttaaaa atttagtgac tgaagaagaa agcaggtttt ataatttgtc
      961 tggtaatatt tgccctggat ttatggatca gattattgaa aatgagtcaa ttgaatcaat
     1021 tgaaaactat gttcctgttg acttaccaaa atagaaaaaa taatcctgtt tttacagtga
     1081 attattttaa ttttgatttt caataaaaat atattacaat aataatcgta atagctaatg
     1141 aaacaatttc tcaatctttt taccactcca aacagggagc gtcaataatt ttgtgtaaat
     1201 aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc
     1261 acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg
     1321 gatactagga aacgctctag tgattcttca ttttgaaact gctcttttct gcggctgtat
     1381 ttcttgattt gtttattgaa agattcgatt gtagaaccag aaggaatgga gaacactttt
     1441 gaactttaca acaaaactac tgttgataaa ttaattccaa ttttagttcc aactgattta
     1501 aatggtcatg atgttttact tcgttttaac ggatttttag gtgaggatta tagataccta
     1561 aaaatttctg atcctcaacc tttaacagaa taataaaaaa cgtgtaatca attatttgat
     1621 tacacgtttt ttatttcaat cttcgttgta gtagtggttg cgacataccg aaaggtaatc
     1681 ttcattacct cctactacaa tctgttcacc tgaataaact ggaataccat caacaatacg
     1741 agcattcatt acagcctttt tattgcaaaa atggcaaata gttttaattt cctcaatttt
     1801 atcagcaaat gtcaataatg cttcggatcc cttgaagaga ttattttgaa aatcattctt
     1861 caatccatag caaattactg gtacttcctc attcattacg ataagtgata gatcaataat
     1921 attctctcta cttaaaaatt gagactcatc gaccaaaata caatcaactt ttccgtactg
     1981 atcaataatc tccactaatt ctccgtacac atccatttca gaagtaattg caatagctgg
     2041 tttcgaaaca cctatacgac tctcaacttt tccaaatcct gatcgagtat ctattgcgct
     2101 tgtcattatg ataactctct tcccttgttc ttcataatta tgagccactt tcagtagctc
     2161 aatactcttt ccactattca ttgttccata tctgtaaaat aattgtgcca ttaaatttat
     2221 tcccttcttt tacaattcaa ttattcctta attccatctg cttttatgca gctgctcgtt
     2281 tattccttga tatgtctata acatctacta tccctaaaac caatttataa gccattagag
     2341 atggtattcc tgaacatctc tcatattgtt ttttgagtgg tatatcatct gaaaaactga
     2401 aggaatcatg aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac
     2461 ttcttcagtc agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata
     2521 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
     2581 gacggtctcc tctaatgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa
     2641 ctcgccattg cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt
     2701 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc
     2761 gtaatcgagg aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg
     2821 gacacggaat aatccattca aatcgccttt gttcgctaga gtctttagga tttctgtagt
     2881 aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca
     2941 gaagggactt ccttttttct atttacacaa aatattttac actctcaaca aaagtatctt
     3001 cgattaatac ttgctcaagt tatgtgcagc tacgaaaaag taggtaccga ggtactttct
     3061 ttttcgcagc tttttttgga cttatgagaa gtacattcgc tggaagaata cgatataaaa
     3121 cgagcttttt cactaaagat attaatagag tgaaagaaac aagcttttgt tgtcagtttt
     3181 ttcacttttc taaaaatgaa aataaaaaat tctggaagtg gagaagaatt ttgcttgcta
     3241 tactttgtct tgtagaaaag aggtagacaa aataatgcaa gctcaaataa gcgattttcc
     3301 ataaccttgg taggaatgac aaattaaata ggaaagggaa gtaataatat gaataaaaaa
     3361 aagttatctc tattcggttt ttttgccctg acagcttcca tggttttgac tgtttatgag
     3421 tatccgacct ttgcaacatc acaattacat ttggttttct ttcttctttt gggaggtatt
     3481 ttatggtttc taccagttgc tttatgtgct gcagaaatgg caacagttga aggatggaaa
     3541 gatggtggga ttttcagttg ggttagtcaa actttaggag aacgttttgg ttttgcagct
     3601 atttttttcc aatggtttca aattacagta ggttttatta caatgatcta cttcatacta
     3661 ggagcgctgt cgtatgtaat taattttcca gagcttaata cagatccatt tatcaagttt
     3721 attggtgtat tagtcatttt ctgggtactg actttttcac agttgggcgg gaccaatcgt
     3781 accgctaaaa ttgctaaagc aggatttgtc attggaattg ttattccttc gatcattctc
     3841 tttggtttag cagcagccta ctttatcggc ggtaatccga ttcaaatggc tattaatgcg
     3901 aaagctttta ttcctgactt ttctcagctt tcaactttag tagtatttgt atcttttata
     3961 ttggcttata tgggcgtaga agcttcagca tcacatatta atgagctgga aaatccccaa
     4021 agaaactacc cattggcaat gtttttacta gtgattttgg ccatcgcctt agatgcaatc
     4081 ggcggtttct cagtagcagc ggttattcca caacatgact tgtctctcag tgctggtgta
     4141 atccaaacat ttgaaacgct tattttacat tttaacagtc atttacattg gttggtaaaa
     4201 gtgattgctc tcatgattgc ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt
     4261 ccatcacgag ggatgtttgc agctgctcag cgaggtctgt taccagtttt cttccggaaa
     4321 actaataaac aagacgttcc agttccgttg attatgatac aaggaattat cgttactatt
     4381 tgggcagcag tcttaacttt tggtggagga ggcaacaatt tatcattctt agtagcaatc
     4441 tccttaactg ttgtcattta tctcgtagga tatttactat ttttcctagg ttactttgta
     4501 ttagttttta agaaaaaaga cttaaaacga acttacaatg taccaggaaa aactgttggc
     4561 aaaacgatta tcggtggaat tggttttatt ttatcagtgt tcgcattgct tatctctttt
     4621 gtaccaccag cttcaattgc agctagtgag caccgaacat accaaatcat actacttatt
     4681 agctttgtag tgactgttct tttacctttt atcatttatg aagtacatga taaaagtgct
     4741 cacacaacta ttgaggaacc tactcacgtg aaagctgaag acgttaatcc cgctgtttat
     4801 ccagctgcta gaggagagca ccacgttaca aaaaatgatg aacatatttt aaaacattag
     4861 gaggaattca ttatgttata cggaaaagat aatcaagaag aaaaaaacta tttggaacca
     4921 atttttggct ctgcaagtga ggatgttgac ttgcctaaat ataagttaaa caaagaatcc
     4981 attgaaccac gaattgctta tcaattagta caagacgaga tgttggatga aggaaatgcg
     5041 cgattaaact tagctacttt ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg
     5101 acccaaacgt tagaaaaaaa tgcaattgat aaatcagaat acccacgaac aacggaaatt
     5161 gaaaaccgct gtgtaaatat gattgctgat ttatggcatg ctccaaataa tgaaaaattc
     5221 atgggaactt caacgatcgg ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa
     5281 tttgcttggc gtaaacgtgc tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac
     5341 ctggtgatct cttctggtta ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg
     5401 gaactgagag aagtcccaat ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg
     5461 gattatgtgg atgagtacac aattggtatt gtaggtatta tgggtattac ttacactggt
     5521 cgttatgatg atatcaaggg tctgaatgat ttagttgaag ctcacaataa acaaactgac
     5581 tataaagtat acattcatgt tgacgctgca tcgggtggct tttatgcacc atttactgaa
     5641 cctgatctag tttgggattt tcaattgaaa aatgttatct caattaattc ttcaggtcac
     5701 aaatatggtt tggtatatcc aggtgtgggt tgggtcttat ggcgtgacca acaatactta
     5761 ccagaagaat tagtatttaa agttagttac ttaggtggag aaatgccaac tatggctatc
     5821 aacttctctc atagtgcagc acaactaatt gggcaatact acaactttgt acgctatggc
     5881 tttgatggtt atcgtgatat tcaccaaaga actcatgatg ttgctgttta tttagccaaa
     5941 gagatcgaaa aaactggtat ttttgaaatc attaatgatg gatcagaatt gccagttgtg
     6001 tgctataagc tgaaagaaga tcccaatcgc gaatggacac tatatgattt atctgatcgt
     6061 ctgttaatga agggatggca agtcccagcc tacccattac ctaaagactt ggatcaatta
     6121 attattcaac gcttagttgt tcgagcagac tttggaatga acatggctgg tgattatgta
     6181 caagatatga accaagcaat tgaagagttg aataaagctc atattgttta tcataaaaaa
     6241 caggatgtga aaacgtacgg ctttactcac taaaattctt cagagaaata taaaagaagt
     6301 taattaacta ataaaaggag ttgtgattac cacacagctc cttaaatcta tttgaataaa
     6361 tatatgatat tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct
     6421 tattgtaagt agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt
     6481 tgaaaagtaa tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa
     6541 ctatcgcaaa gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt
     6601 tgtttacaaa tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt
     6661 cgatagtata aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc
     6721 cgtttatgct catttaaaag acttaaacta atggttagtt tacgaataga attctcattc
     6781 caacgcagaa ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag
     6841 tacaaagcgc gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata
     6901 tcagcccaaa gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct
     6961 tcagatatat tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct
     7021 gctacatggt tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat
     7081 agataatttc tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat
     7141 aaagagaaag aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg
     7201 gcaactcttt tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg
     7261 atagcgatac ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat
     7321 gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta tttagctgga
     7381 tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga taatcctttt
     7441 caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa aaattaatca
     7501 actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca gctatattac
     7561 tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca gagcgctgat
     7621 agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact gtaaagctac
     7681 ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg cattccaagt
     7741 cgttagtgac agtgagtggg caaacgttca attgatctgc tatttcgcga atctgaatgg
     7801 tccctttttc gtgtaattgc tcacaaatgt aaacttgacg aaaaatactt ttctccagat
     7861 aggtttccaa catagtactc ctcctagtca ttttatacat aactattata actctaccaa
     7921 tcggaatagt cctgtaatat attgaatcaa aaatataaat tataaaataa ttagatgggg
     7981 tatttggttt aagtatccct tatatagtaa taaatttata ggtgtgattc tcgaattaat
     8041 acccgaaaat gtgagagaat ttttatggat tttttaggat acaaattaat tatttttgct
     8101 tttatcctga ttaccagtcc agtactgtcg gtatcccatt ttgcagcaaa atcatgttga
     8161 taaagtgaca agaagactga tgagatatat tatccaaatt gagaagataa ttaatcggat
     8221 tcttgagtat accctacttt ttaagaaagt agggtataca agcgtatgaa taaagacata
     8281 actttccctg tgatggacgt tcggcttgtt ccaattacca aaatacaggc gaacgattat
     8341 aatcccaatc gagtagcgtc tccggaaatg aacttattgg agctttctat cattgaagat
     8401 ggctttactc aaccattagt ttgttattac aaggaaaaag aagagaagta tatcttggtt
     8461 gatgtgcttc ataggtatcg tgttgctaag gaaagactca acttaaaagt tgtaccggta
     8521 acagttattg ataaacccat agaagaacgt atggcttcaa caattcgaca caatcgtgca
     8581 agagggaccc atgaaataag aggaatggaa attgtcatcc aaagattagt aaaaaatgga
     8641 tggtcagaca agagaatatc caaagaatta ggcatggaca ttgaagaaat ttttcgcttt
     8701 aagcaagtgt ctg
//
LOCUS       DAYYKC010000110         8587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000110 DAYYKC010000000
VERSION     DAYYKC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.111"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1512)
                     /locus_tag="ACHBBW_002566"
     CDS             complement(46..1512)
                     /locus_tag="ACHBBW_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9154197.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1899..2495
                     /locus_tag="ACHBBW_002567"
     CDS             1899..2495
                     /locus_tag="ACHBBW_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9154198.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2553..3035)
                     /locus_tag="ACHBBW_002568"
     CDS             complement(2553..3035)
                     /locus_tag="ACHBBW_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154199.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3141..3338)
                     /locus_tag="ACHBBW_002569"
     CDS             complement(3141..3338)
                     /locus_tag="ACHBBW_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154200.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3633..4391
                     /locus_tag="ACHBBW_002570"
     CDS             3633..4391
                     /locus_tag="ACHBBW_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9154201.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4451..4879
                     /locus_tag="ACHBBW_002571"
     CDS             4451..4879
                     /locus_tag="ACHBBW_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154202.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4925..4997
                     /locus_tag="ACHBBW_002572"
     tRNA            4925..4997
                     /locus_tag="ACHBBW_002572"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4958..4960,aa:Gln,seq:ctg)
     gene            complement(5147..5518)
                     /locus_tag="ACHBBW_002573"
     CDS             complement(5147..5518)
                     /locus_tag="ACHBBW_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9154203.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5733..5918
                     /locus_tag="ACHBBW_002574"
     CDS             5733..5918
                     /locus_tag="ACHBBW_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154204.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6001..6189)
                     /locus_tag="ACHBBW_002575"
     CDS             complement(6001..6189)
                     /locus_tag="ACHBBW_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154205.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6213..6476)
                     /locus_tag="ACHBBW_002576"
     CDS             complement(6213..6476)
                     /locus_tag="ACHBBW_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154206.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6634..6891
                     /locus_tag="ACHBBW_002577"
     CDS             6634..6891
                     /locus_tag="ACHBBW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9154207.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6962..7153)
                     /locus_tag="ACHBBW_002578"
     CDS             complement(6962..7153)
                     /locus_tag="ACHBBW_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154208.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7294..7614)
                     /locus_tag="ACHBBW_002579"
     CDS             complement(7294..7614)
                     /locus_tag="ACHBBW_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154209.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7637..8071)
                     /locus_tag="ACHBBW_002580"
     CDS             complement(7637..8071)
                     /locus_tag="ACHBBW_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9154210.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8406..>8587
                     /locus_tag="ACHBBW_002581"
     CDS             8406..>8587
                     /locus_tag="ACHBBW_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9154211.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1695 c         1361 g         2812 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt ggtttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
     1801 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
     1861 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
     1921 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
     1981 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
     2041 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
     2101 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
     2161 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
     2221 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
     2281 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
     2341 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
     2401 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctgaggaa aaagaataca
     2461 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
     2521 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
     2581 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
     2641 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     2701 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     2761 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     2821 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     2881 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     2941 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     3001 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     3061 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     3121 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     3181 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     3241 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     3301 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     3361 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     3421 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     3481 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     3541 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     3601 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     3661 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     3721 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     3781 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     3841 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     3901 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     3961 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     4021 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     4081 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     4141 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     4201 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     4261 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     4321 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     4381 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     4441 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     4501 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     4561 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     4621 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     4681 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     4741 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     4801 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     4861 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     4921 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     4981 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     5041 atctttttgc agggggatta ttgtattatt tttactactt gtatttagtt tacataatta
     5101 aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac ccaataattg
     5161 ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct atgtgtgctc
     5221 ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca ttaaattcaa
     5281 ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct gtaccatatt
     5341 catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca actaatggat
     5401 ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa tatttcagtc
     5461 caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc actttcatct
     5521 ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc atccgtccca
     5581 agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta gtaaaaatga
     5641 tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg tccatgtaaa
     5701 aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta tttcaaaaag
     5761 tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc gaagaacata
     5821 aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat gtaaatatcg
     5881 acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca cttttgtaaa
     5941 atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga atacttcata
     6001 ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg aggcttttcc
     6061 caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca ttaattcttc
     6121 tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa gttgttcata
     6181 tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat aggttcatat
     6241 gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat tttttctact
     6301 gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa
     6361 ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac aacatagtaa
     6421 atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg atccatgaaa
     6481 atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc aaaatcgata
     6541 cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt
     6601 gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca atttgttttt
     6661 tatattcctt tttttattat gataagcatt cttttctatt acataaaatg gacaaaaaag
     6721 aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca aattttttct
     6781 tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt gacgctttca
     6841 gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg aatccctcgc
     6901 atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt aatcgttttt
     6961 tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct tgttttaatt
     7021 ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc ttttcgatca
     7081 ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc tgtaatactt
     7141 ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt tatttaagtt
     7201 atacgcatta ttctagtttc atcccaaata accttttttc atttttactc ctcgaactaa
     7261 tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt cattctgctc
     7321 ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat catagctgta
     7381 taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt tagctgtgac
     7441 agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac ctttttttcc
     7501 tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat
     7561 tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa ccattttcta
     7621 ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt gatacgacat
     7681 aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc gctcccataa
     7741 cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt cttggatctc
     7801 cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca ttgatttttt
     7861 tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa aaggcatagg
     7921 cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg gcttcattgt
     7981 cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg
     8041 ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc ttttattgta
     8101 acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga tgaacaaaag
     8161 caatttcttt caagttattt atacatttct tttctcatca gaacacctgt tctaatccca
     8221 ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac atcattctgt
     8281 tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga aatattttca
     8341 cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa taaaggagat
     8401 ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc ggcgctgcat
     8461 tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca ggtgatactc
     8521 tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc attgcagaat
     8581 caaattc
//
LOCUS       DAYYKC010000111         8584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000111 DAYYKC010000000
VERSION     DAYYKC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.112"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..860)
                     /locus_tag="ACHBBW_002582"
     CDS             complement(231..860)
                     /locus_tag="ACHBBW_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9154212.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(853..1971)
                     /locus_tag="ACHBBW_002583"
     CDS             complement(853..1971)
                     /locus_tag="ACHBBW_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9154213.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1973..2605)
                     /locus_tag="ACHBBW_002584"
     CDS             complement(1973..2605)
                     /locus_tag="ACHBBW_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9154214.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2616..4433)
                     /locus_tag="ACHBBW_002585"
     CDS             complement(2616..4433)
                     /locus_tag="ACHBBW_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9154215.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4446..5210)
                     /locus_tag="ACHBBW_002586"
     CDS             complement(4446..5210)
                     /locus_tag="ACHBBW_002586"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9154216.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5238..5936)
                     /locus_tag="ACHBBW_002587"
     CDS             complement(5238..5936)
                     /locus_tag="ACHBBW_002587"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9154217.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5948..6727)
                     /locus_tag="ACHBBW_002588"
     CDS             complement(5948..6727)
                     /locus_tag="ACHBBW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9154218.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6743..7687)
                     /locus_tag="ACHBBW_002589"
     CDS             complement(6743..7687)
                     /locus_tag="ACHBBW_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9154219.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7733..8512)
                     /locus_tag="ACHBBW_002590"
     CDS             complement(7733..8512)
                     /locus_tag="ACHBBW_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154220.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2304 a         1780 c         1464 g         3036 t
ORIGIN      
        1 tgagataaaa ttcgcattac ggatatattt ttttatatcc gatcgttttt atcacatgac
       61 gatttttaat catatgcctt tctttactgg atagaaattg ttttcttata tgtgagtaat
      121 cagatacaac attaaaatat tcgatactgt tttccgtcag aatttcctct aatatattcg
      181 ttcgactgat ataatcaaaa ggatgaataa gcagccactt ttttttattc tcactcatct
      241 atcttcgcac cctcaaattt ctcagtagtc ccacttgcta ctgatgttat tccatcacgg
      301 acaataactt tctttagcgt taaaaaaaat atttttatgt ctttcatcat tgaaatgttc
      361 tccacatatt caatatcttt ttcaaatttt tctttccaac taattgcatt acgaccactg
      421 acttgagcta atccggttaa acctgggcgc acaaaatgtc tttttcgttg ttcattactg
      481 tatagtgaaa gatactcaac taacagagga cgcggtccaa taatactcat atctcctttt
      541 aaaatattcc ataattctgg tagttcatca agactagtcg aacgcaatat tttaccaaat
      601 tgagtcaatc tgatatcgtc tgataataaa ttaccttctt catctctttg atctgtcatt
      661 gttcgaaatt tatacatcag aaaaatcttt tcatgttttc caggtcgttg ttgtttaaag
      721 atcactggcg atccaagctt tattcgaact aggattgcaa caaacgtcat cagtggccat
      781 aacattatta aacctaaaat tgaaaaaaga atatctatca aacgtttaaa gaacttttcg
      841 tagatacctc tattagacat tttgcatcac tcgcttaatt gtattagtta ctcgatacaa
      901 atcatcatcg gtcattttag aatctgaagg taaacagata cctttttcaa acaatgtctg
      961 agcaacatct gtaccaatat actcatactt atcgaaaact ggctgtaaat gcattggttt
     1021 ccatatcggg cgggcttcta tattattttt atctaactca tcaataagat gattcgtatt
     1081 tattcccttt actgttatga cacttaacca ataatttggt tcattccaat tattttctgg
     1141 catgaattgt atatttgaca atttagacaa ttctttttta taaaaactat gaatatatcg
     1201 ttttttctca attctttcat ttaaaacttt caattgtcct cgaccgatac ctgcaacgat
     1261 attgctcatt cgataattat agccaatttc attgtgttga taatggcgtg ccttctcacg
     1321 agattgcgta gcccaaaacc tcactttttc caccttttct ttgtcattag acacaagcat
     1381 tccgccacct gaagtcgtga ttatcttatt tccattaaaa gaataaattc catagtcgcc
     1441 gaatgtacca gtatattttc ctttatatgt tgttcctaat gattctgcag catcttcaat
     1501 taaaacaaca ttatgttctt tgcatatttt agtaatttca tctagttcag ctgataaacc
     1561 gtacaaatgc actactaata cagcttttgc gttaggatat ttttctagcg ctgttcgcaa
     1621 atgttcaggg ttcatattcc aagtttcgta atcactatca acaaatactg gaatagctcc
     1681 ttgatagatg attggattgg cagtagctga gaacgtcaaa gactgacaaa tcactatatc
     1741 atctttaccg accccagccg cttttaaagc tagatgtata gctgctgttc cagaagatag
     1801 tgcagctgca tgactagcac cgacatattc tgctaattcc tcttcaaaac cattgacatt
     1861 catacccaaa ggagccaccc aatttgtatc aaatgcttct tttacatact cttgttcata
     1921 tccttcatca ctcatatgag gagatgctaa taatattcgt ttattttcca acttaatccc
     1981 atctttctat gactttggta ggtacgccaa catttttagt cttattatta atatttttta
     2041 ttactacact tcccgcacct ataatgttca tatttccaat tcttagattt tgtattattt
     2101 tagtacctac tccaatttca tttttatctc ctaatagaac atgtcctgaa atatttacac
     2161 ttggaaaaat tgaattataa ttaccgattt gagtatcatg tccgatagta gagccgatat
     2221 tgatcatatt gaaatttcct aaaactacgt cagcagtgta agtcgtatag ggcatcaaaa
     2281 tattgccaat tcctaactga acatttatgc ccactagtgc acttgatgca actaaattag
     2341 ggaaaatcaa tttattattt ttatttatct tttgatatat tttctctttg atatctggcg
     2401 aagcgattcc cagaaaaata gcagtttctt cttctaattc taataacgag tctattgttc
     2461 ccaatacagt ttttcccact aattcttgat tttgcatttt taacgaatca tcgacaaaac
     2521 caatcaattc atatctagag aaactttcta gtagaaaagc aatctctttg ccaaatccac
     2581 cagctcctac aataattgct ttcactttac tccccctact tattcgaagc attcgcaaaa
     2641 gtgacaatgt ctttagccaa ttgttcatcg tcttgcggca ggctcttgac aaattccatc
     2701 actgtttgca tggaataacc tttgatgttt cccacaaaga tcttctcgta aactgcttca
     2761 tcgtttcttt ccttatctaa caatagttct tcatatagtt tttcccctgg acggatacct
     2821 gtttcaatga tttcgatctc gtcttcgcta tatccgctca atcggatcat gttttttgct
     2881 agatctacga ttttcactgg ttcactcata tctaaaataa agatttcgcc gccttttgct
     2941 aacgccccag attgaataac taaacgactt gcttcaggaa tcgtcatgaa atatcgggtc
     3001 atacgaaagt ctgttacagt gactggccct ccttgagcaa tttgttcccg gaatactgga
     3061 atcacactac cgcgagaacc aaggacattc ccaaatctga cagctgagaa tttcgtacat
     3121 ccttcttcat tcaacccagt cacgatcatt tcagcgattc gttttgttga acccatgacg
     3181 tttggcggat tgttggcttt atctgtcgag accatgacga agttttttac tttcgctgct
     3241 ttggctgctt ctgctacatt ttttgtaccg aaaatattgt ttttgactgc ttcttttggg
     3301 ttatattcca tcaatggtac atgcttatga gcggctgcat ggtagacgat atctgggtgg
     3361 tattgttcca tgatctcatt gattttcttg cgatcttgga tatccgcaat gatcggcacg
     3421 atttctgtag gacacttttg atgatgcgtg cgtaattcac ggtcaatcaa gtaaatcgag
     3481 ttctccccat gacctaaaag taatagtttt gcagggtcaa attggatgat ttgccggcag
     3541 atttctgaac cgattgatcc tcctgcaccg gtgacaagaa tgactttgtt tgtgatctgg
     3601 tctttgatgg attcgatatc caactctacc tcatctcttc ccagtaattc cactacgtcg
     3661 atggttttta attttgatac gctgattttc cctgaggcaa gttcttccat agaaggcatc
     3721 gtattgactt tcacatgagc agattcgact aactcgaaga ttcttcgtaa tttttttctt
     3781 gataaagaag ggatagcgat cgttaccatt tggatgtcat atttttcgat caattccggt
     3841 atatctttta atgaccccag tacttttttt cctgaaagat aggtatttct tttattcgga
     3901 tcatcatcca cgaatccaat gacatggata tcttgagccg ttttagaacc aagaaagctg
     3961 ttgtataaaa tacgtccacc ttcgccggcc ccaacgatca aggtattttt agcggaatct
     4021 gttgatagat acctcatatt ttttgtttcg atatagatac gccagatcaa tcggctgcca
     4081 atgatcagta atagcgaaag taggtatgca aagatcacta aacgtaaact gtattgttta
     4141 tttatgaaaa gcaatatgat gatacttgag gccgcacttg cagataacga accaaaaatc
     4201 gcaatgatct ctcttaaatt tgtataacga ttgattcgag taaaaacttt gaataaacta
     4261 ccatagaata aataaaaacc aatcgacaaa ccgatagaga ttaaaataag gtccattggt
     4321 atagctacaa acggcttcat gaatttagct gctgcaatat tcgcagcaat caacaacaag
     4381 ctgtccacaa ataccaatgt ggcaattttt cttcttcttg ttaatacaaa caccctacat
     4441 tccccctaaa ataaaccaaa cttcttcttt tttacctctt catagactgg ataactgatc
     4501 tcatccccgt tgatcaggtc ctttgcgact tgtttcatct gtgctacttt ttccttacca
     4561 aagtccttgg cgatttgctc ataacattct tttaagtaaa aggtacgttt agagacccca
     4621 tgggcatcgg aagcagccat ttgcacgagg ttatgtttga ccatcatttt ggctgttttc
     4681 tggatgtctt ttccaaatac accgacataa cttggtgcgg tgagttgagc caagcatccc
     4741 atatctagga atggcagcaa atgatttggg tcttctcgaa aatgcgcgtt gcgttctgga
     4801 tggacgatca ctggtgtctt tcctagttgt aacaactcaa aaaagagcca ttctgcatat
     4861 aacggcactt ccagtgtcgg aaattcgatt agaagatatg tatcatctaa atctgtaaat
     4921 aaaatttcat ctcggtggat gtcttcaatc agtgttccgg taatacgtac ttcttgtcct
     4981 tccaataacg tcaaaggcaa ttgtcttttc tctaattctg cttgtaacga agcgactaga
     5041 gaaatgactt gtgatttttc attgctgtat tttccattat tgtgatgggg tgtgcataaa
     5101 atatgtgtga tcccttgttg aatcgctttt tcagccatgg cgatactggc atctatgttt
     5161 tctgctccgt catcgattcc tggcaggata tggcaatgta gatcgatcat ttctcttgtc
     5221 tcctctcttt ttatttctta attcccgtag taatagccag catctctgct gtgttctgct
     5281 ccattgtata caacaccaag gactcgcgct tgtaccatat ctaacagttc ttttgcttta
     5341 gtcaatgatt cttttcgtga cgtattttcc cgtacgacca ataaggtgcc atccgttttt
     5401 gaagacatga tctgtgcatc tgttacggca acgactggtg gcatgtcgaa aatcactaca
     5461 tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc ttggcgaact tagtagttct
     5521 gatggatttg gtggtttagg tccacttggc agaatcgaca aattgtcgac tggtgttcgt
     5581 tggatctcat ctgctacact tcctgatgaa ctaagaacag tacttaatcc gcttgcgttg
     5641 ttcaacttga acgttttata gatgacgggc ttacgtaagt ctgcatcgac tagcagtacc
     5701 cgttgaccag atttggcaaa aacaacggca atgtttgctg cagtggtgga ttttccttct
     5761 ccaggacctg atgaagtcac gacaatcgtt ttgatttgct gaccagctgc ggaggcaaac
     5821 tggatattcg tccggatcgt acgatattgt tcggcgattg gtgaagatgg gtttgttaat
     5881 gtgatcaaac tgactgcatg agtttgagtt gctttttgtt tctgtgttcg tgccattttt
     5941 cttcctccta gactcttgag cggcttctgc gtgatggggc tgttttatcg tttgtagctt
     6001 ttggtcgtgc aggagctgat ccttttttac ggataccagc gttcaattct tttgctgaca
     6061 tttctggaac tgttcctaag attgggaatc ctaatgtatc cgtcacatat ttttcatctt
     6121 tcactgtccg atcaagtaat tcgaataaga atgccagacc tacgcctacc atcattccta
     6181 acactaagcc aatcactaga ttcaatttat tatttggcga aacaggtgtc gtattggcaa
     6241 ctgcacctga aataatcgag attttatcga cattcatcac gtctttggcg ttttcttgga
     6301 aaacttgtga agttgtatta gcgatctgtt gagcagtcac tgcattcgtg cttattgcct
     6361 ggatcgagaa catttgtgaa ttttgtgact gattgacgga gatcgcatct ttgatttgtc
     6421 cagcagtcat tttcacattg tcttctgttt ctaaacgatc tttgacttca tttagtacta
     6481 aatcactgac gatcatatct ttatacgtat tgatcatttg caggttcgta ttgacatcat
     6541 tgacatttgc tgtgtctgat tgtggcagtg tcacgatcaa ttgtgcttgt gaactgtatt
     6601 ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc caagccagca aacattgaga
     6661 tgatgattgt taccaagtgt ttgcgtaatg tttcaaaaat ttctttcaag ctgattgtct
     6721 cttccatttt ttctccactt tcctatttat tctctagatt catttgatct tttaattctt
     6781 ttttcacacg atcaagttct tgatcgctga ctttttgata ggagatatta tcttgcatga
     6841 agccttctcc ttgcagctga tcttgcttga tcgtaccaaa tgcctcccga taatctaaag
     6901 cgattttttt catttcatca aagctcatgt ctgttttcat attcgactct agagcattca
     6961 aaacggattg atagttgctg acaccgttta aactcaatac tttttttgct acaccttcta
     7021 cgatctttct ttgacgctct tgtctaccat agtcaccatt tggatcttca taacgcatac
     7081 gagaataaga taatgcctga tccccgttca atgtaatttt accgattgga aagtcatagc
     7141 catcttgtga gaaagtgagg tcattattga cttcgatacc accaactgca tcaacgagtt
     7201 cctttagccc tttcatattg atagagacat agtgatcgat tgggatgtct acatattttt
     7261 gtagggtatc catcgccatg gcagctccgc cgaatgcgta agcgtggttg atcttgtctt
     7321 ttgtgtcacg acctacgatg tcgacatacg tatctcgagg aatgctgacg atcgttgtct
     7381 gtttgtcttc aggactgact gtggcaacca tcatcgtatc tgatcttcct tgatcgaccc
     7441 gacctaaatc tccagtatca atgcccatca atagaacaga gaaagattct tgtttgtcca
     7501 gatcaactgg tttcgttcgt tctccttggg ttcgttccac tgattcgtaa gttttctcaa
     7561 tagaatgtga gagatccaga tataattttg ctcctactcc tacaattgct aatactaata
     7621 cgataagaac tgctaaaata atcagaaata tttttttccc tcttgatttt cttcttcgtc
     7681 ttgccatagt ttcctccgtt tgctaacctt gcttgaatca acgttttaac tgtcaataac
     7741 ttatattgct atagcccatt tgctgagcaa cagtttgtgc ttttgttgcg tatgctgcgc
     7801 ttattttttc attccggatc tgctctattt gactgagcgc cgtcaaatat tgatcgtaag
     7861 tagcggtggc agtcacttga ccatccttca acatcgtatc gaataactga tctagttgcg
     7921 tgacttgcgc gatctgagta tcagcgaatc ctagatattg cataacaacc gttttccaag
     7981 gactatcttt aaaaaagctc atattttcac gtacacgagc gacatctgca cttgtcacct
     8041 tttctttgat gacgacatcg tctacagctt gctgccagtt tgatacattt ttagtaaata
     8101 aatcgttgac agctgtctgg atcttcagtt ttgattctgc actatctaac tgtgtcacga
     8161 ttgctttctt ctcatcttgg attgcaacag cttctttagg caattctgat tctttgatgt
     8221 cgtaatcatc tgctgaaagt ttgacagagg atagcttatt tgacaaactt gtgattgttt
     8281 cttctgttat ttcagggttt agaaaatttt tttcttttgt actatacagc ttgtctactt
     8341 cttttgataa agaagtcaag gtgtcttttt gattggtgat ggcagattct gcataattga
     8401 tccgttgctg atgttgccgc tgattattga tatggtaacc aaacaaacag atcgcaatag
     8461 cacctgcacc taccgccaca gcagtcaatg acttgacgaa tggcttttcc atagattcac
     8521 tcctaagtat tgatgataga taaattaaga tataattcac atacataaaa atgacagctt
     8581 ttta
//
LOCUS       DAYYKC010000112         8430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000112 DAYYKC010000000
VERSION     DAYYKC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.113"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHBBW_002591"
     CDS             476..1339
                     /locus_tag="ACHBBW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9154221.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHBBW_002592"
     CDS             complement(1468..3090)
                     /locus_tag="ACHBBW_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9154222.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHBBW_002593"
     CDS             3339..3860
                     /locus_tag="ACHBBW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9154223.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBBW_002594"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBBW_002594"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9154224.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHBBW_002595"
     CDS             5941..6759
                     /locus_tag="ACHBBW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9154225.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHBBW_002596"
     CDS             complement(6862..7059)
                     /locus_tag="ACHBBW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154226.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHBBW_002597"
     CDS             7228..7806
                     /locus_tag="ACHBBW_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9154227.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHBBW_002598"
     CDS             7901..8038
                     /locus_tag="ACHBBW_002598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154228.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2548 a         1509 c         1649 g         2724 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt
//
LOCUS       DAYYKC010000113         8304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000113 DAYYKC010000000
VERSION     DAYYKC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.114"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..238
                     /locus_tag="ACHBBW_002599"
     CDS             <1..238
                     /locus_tag="ACHBBW_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154229.1"
                     /translation="QVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYLAGQI
                     YTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            219..665
                     /locus_tag="ACHBBW_002600"
     CDS             219..665
                     /locus_tag="ACHBBW_002600"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9154230.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1008..1331
                     /gene="relB"
                     /locus_tag="ACHBBW_002601"
     CDS             1008..1331
                     /gene="relB"
                     /locus_tag="ACHBBW_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9154231.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            1651..1878
                     /locus_tag="ACHBBW_002602"
     CDS             1651..1878
                     /locus_tag="ACHBBW_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9154232.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1894..3060
                     /gene="hemW"
                     /locus_tag="ACHBBW_002603"
     CDS             1894..3060
                     /gene="hemW"
                     /locus_tag="ACHBBW_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9154233.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3295..4338
                     /gene="hrcA"
                     /locus_tag="ACHBBW_002604"
     CDS             3295..4338
                     /gene="hrcA"
                     /locus_tag="ACHBBW_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9154234.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4432..4995
                     /gene="grpE"
                     /locus_tag="ACHBBW_002605"
     CDS             4432..4995
                     /gene="grpE"
                     /locus_tag="ACHBBW_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9154235.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5046..6875
                     /gene="dnaK"
                     /locus_tag="ACHBBW_002606"
     CDS             5046..6875
                     /gene="dnaK"
                     /locus_tag="ACHBBW_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9154236.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7026..8192
                     /gene="dnaJ"
                     /locus_tag="ACHBBW_002607"
     CDS             7026..8192
                     /gene="dnaJ"
                     /locus_tag="ACHBBW_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9154237.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2892 a         1377 c         1794 g         2241 t
ORIGIN      
        1 tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta attttgtaaa
       61 gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag aagtatattt
      121 agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca gaaaagtata
      181 taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag atatataacc
      241 aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta gaggaagaag
      301 caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac gaagaggagt
      361 tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa tatgttaaac
      421 gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata aaagacggtg
      481 taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa gatgtgattg
      541 aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt gagttacctg
      601 acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt tctaaaatac
      661 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
      721 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
      781 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
      841 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
      901 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
      961 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
     1021 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
     1081 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
     1141 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
     1201 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
     1261 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
     1321 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
     1381 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa ttggaatcta
     1441 gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa taaggacaaa
     1501 attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt
     1561 ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg taagcaaagc
     1621 gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat ttttgcagat
     1681 tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc tgtaaaagca
     1741 ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct tgattctaaa
     1801 tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg
     1861 cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa tcccgcagaa
     1921 aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta ttactgtgac
     1981 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
     2041 gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact ttatgtagga
     2101 ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc aggtgctaga
     2161 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc tggtgatttg
     2221 acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc catgggagtg
     2281 cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc agaagatgtg
     2341 tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
     2401 tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa agctttggct
     2461 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
     2521 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaatgttt
     2581 gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt
     2641 gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga ccactatttt
     2701 gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa caaaggacca
     2761 atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga agaagaaaaa
     2821 ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa aaaaacaggg
     2881 attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat
     2941 gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg tgtttatttg
     3001 acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt tgaaaaatag
     3061 tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa
     3121 tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat tagcactctt
     3181 gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata
     3241 tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta
     3301 acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg
     3361 cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc aacgattcgc
     3421 aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg
     3481 cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc
     3541 gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc
     3601 aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt
     3661 tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat
     3721 aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc
     3781 actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac
     3841 cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg
     3901 attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga caccgttctt
     3961 ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt ggattatgag
     4021 ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga
     4081 cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta
     4141 ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat
     4201 ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt
     4261 ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta ccgctcgctt
     4321 gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc
     4381 ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa
     4441 aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc
     4501 gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa
     4561 ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca
     4621 aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat
     4681 ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg
     4741 tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt
     4801 aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatcct
     4861 aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt
     4921 gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc aactatggtc
     4981 attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt
     5041 taattatgag taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat
     5101 tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg
     5161 ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga
     5221 caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag
     5281 atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca
     5341 aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag
     5401 cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag
     5461 aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa
     5521 cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta
     5581 tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag
     5641 gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa
     5701 acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa
     5761 aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag
     5821 caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat
     5881 taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta aaagatgcag
     5941 gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg
     6001 cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg
     6061 atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag
     6121 atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt
     6181 tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa
     6241 ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg
     6301 ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg
     6361 gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg
     6421 cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa
     6481 cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa gcagataaag
     6541 ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc tctgtggaca
     6601 aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag
     6661 ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg aaaactaaac
     6721 gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagctgcac
     6781 aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg
     6841 ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag
     6901 gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt
     6961 tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa
     7021 agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag
     7081 aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata
     7141 aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg
     7201 acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat ccaaattacg
     7261 gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg
     7321 gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa
     7381 atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga
     7441 tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg
     7501 gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta
     7561 cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa acttgtgatg
     7621 tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac
     7681 atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac
     7741 aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt
     7801 atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact
     7861 atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg
     7921 tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt
     7981 tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag
     8041 taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg
     8101 ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta
     8161 aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat aaagtagact
     8221 tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
     8281 acaatcatac gttcgcctgg tttt
//
LOCUS       DAYYKC010000114         7879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000114 DAYYKC010000000
VERSION     DAYYKC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.116"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHBBW_002608"
     CDS             <1..107
                     /locus_tag="ACHBBW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM9154238.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHBBW_002609"
     CDS             complement(176..904)
                     /locus_tag="ACHBBW_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9154239.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHBBW_002610"
     CDS             complement(917..2197)
                     /locus_tag="ACHBBW_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9154240.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHBBW_002611"
     CDS             complement(2338..2940)
                     /locus_tag="ACHBBW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9154241.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHBBW_002612"
     CDS             3185..3391
                     /locus_tag="ACHBBW_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154242.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHBBW_002613"
     CDS             3703..4422
                     /locus_tag="ACHBBW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9154243.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHBBW_002614"
     CDS             complement(4503..5186)
                     /locus_tag="ACHBBW_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9154244.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHBBW_002615"
     CDS             5405..7591
                     /locus_tag="ACHBBW_002615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9154245.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARPPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2830 a         1318 c         1475 g         2256 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgaccgcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
     7861 tattttatta gctaataat
//
LOCUS       DAYYKC010000115         7815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000115 DAYYKC010000000
VERSION     DAYYKC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.117"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..323
                     /locus_tag="ACHBBW_002616"
     CDS             24..323
                     /locus_tag="ACHBBW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154246.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            588..1463
                     /locus_tag="ACHBBW_002617"
     CDS             588..1463
                     /locus_tag="ACHBBW_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154247.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1460..1678
                     /locus_tag="ACHBBW_002618"
     CDS             1460..1678
                     /locus_tag="ACHBBW_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154248.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1665..4292
                     /locus_tag="ACHBBW_002619"
     CDS             1665..4292
                     /locus_tag="ACHBBW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9154249.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4307..4525
                     /locus_tag="ACHBBW_002620"
     CDS             4307..4525
                     /locus_tag="ACHBBW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154250.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6044..6358
                     /locus_tag="ACHBBW_002621"
     CDS             6044..6358
                     /locus_tag="ACHBBW_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154251.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6411..7184)
                     /locus_tag="ACHBBW_002622"
     CDS             complement(6411..7184)
                     /locus_tag="ACHBBW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154252.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7289..7534
                     /locus_tag="ACHBBW_002623"
     CDS             7289..7534
                     /locus_tag="ACHBBW_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9154253.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
BASE COUNT         3019 a         1004 c         1469 g         2323 t
ORIGIN      
        1 ttaattaaaa aggagaggtt tttgtggaag caataaaatt aatgatttca gaagaacagg
       61 aggaaatgtt tctaaagcag ttttatcaaa ttgctacaaa agctattgaa aaggctcaag
      121 aaaatgcggg gatgacaagg gaatatctca atcaaaaaga aatggcggaa tacttaaatg
      181 tgagtgaaaa ctttttaaaa aatgctgtga gaaatgaggg attgcccgta gttgaattag
      241 gggcaagaaa attttattcg aaaaaggcag ttaatgagtt tatgtttgct cgaatgaaaa
      301 gaggggaaaa tattggaaga tgatgaagta gaaattataa aaaaatgctt taaaaaaaga
      361 aaaaaagtgt tcccaaaagt ttgcagactg ggggaacact caataataaa gtgctgagaa
      421 aactttattt atagctatta tatcaaaaaa gaaggtgaaa aggtgttttc ttctggaaat
      481 gttgtagttg acaaacttag agaaataaat tttgacggaa atgtaatcca cacacatggt
      541 acgatcaatt aagaaaaaaa acgaaaaaag gtgttgagaa gccctatttg aatgcaatag
      601 ttatcttagc agaaattacc tattggtata gacctaaaaa aatttttaac gatgatggtc
      661 agctacttgg ttatcaaaaa aagtttcttg aagatatttt gcaaaaatca tataagcaat
      721 taagcaaaaa aacaggatta tcagaacgag ctataaaaga tgcaattgtg tacttagaga
      781 aaaaggggat tatcaaaagg gtttttagaa atatttatta tgagaaaaga gcagttacat
      841 tatcaaatgt tatgtacatt gttttaaatg tacataaatt gatagaaatt acttttccta
      901 ctttggattg cgaaaaaaag gagtatatgc cgaaaattcg tcagaggggt atcgaaaaaa
      961 agagatatgt ctctttaaaa aaaggtagag gtatgtacaa atttgtatat acgtatacaa
     1021 agactacttc acagaacata tcaaagacta ctctcgttat ggaagaagag gatattaata
     1081 taaagaggat ttacagtaag attagtgttc tttatgacgc aaataacgaa gatggtcgaa
     1141 aattatttca agtgtatcac tatttgagaa acgctaatgt agatacagaa gatatcagaa
     1201 aaatcattga atatttatct gaaaatactc agtatcttat tgctgagtgt attataaaac
     1261 aacatgaagc atgctttttt caaagaaaaa ctgaagaagg gttatacgat tatgtaacat
     1321 attttttgaa tggacttcag aaaagatata aaagtcaatt tttagaaaat gtagagccta
     1381 tagatcagtt caaaaaagct ttcgaaatca aagagttacc tgaaattact atgcataatt
     1441 ggttgaaagg agaagaatca tgattaaaaa tcgcttaata gatcaaatgt ggtgtcttga
     1501 tcaaaataca gatactctcg aattaatcga gagtatctgc ttcaacacag tagttctcga
     1561 tttaagagct gaaaatgaca attgtgtaac tcatatcata attaatcaaa aaatggctca
     1621 gcagctctct gagagcctaa gaaaatgggc tgaaggagaa aactatgaaa gcaattaaag
     1681 aaaaaatcgt tagtagaaaa ataaactggt tagcactcag tgtacttgtt ttaatgatta
     1741 gcgttgtttt tacaagtaat attgttgaag ctagcatttt caattggttt gatgacacaa
     1801 aaagtcaaac agagtttttg aataattctc tttatagacc ttacctaaaa aaatacaatg
     1861 gagatttcct tagtcagttc gggatttggc ttggatgggc tgtggtaaaa ggcatgttta
     1921 ctgtgactga ttcgattcaa aatatgattc cagatgtgtt ggatttgttc aatttcattg
     1981 aaagtacagg gctaaataat gtttatcaat cagtcataaa tacaattgtc gttggattaa
     2041 tgatcctttc attgatgttt gttggctaca agatgattac tggaaaagga acaatagatt
     2101 taagatctgt tggaatgaat atagtgatgt ctgtagcgtt aattctttta atgccaacaa
     2161 tgatttctag cggtattcaa tttgccaaaa tattttacaa tgatgctact acgattacaa
     2221 attcagatga cggagtcgct tggtcattga taaaacaagg agtaacagat ctggcatata
     2281 ttaataaaac agatcaatac tcaagcattg ataaaacgga ggatcgaaat aagttaacac
     2341 gaaaaaattt tcaacagaca gatttaacac aagtgttgac ggataaagtg attgataaat
     2401 tggaaaaaga aaatccagct gcggataatc ttcgctatga attagttgaa aattctaata
     2461 atgaatttgt tgccacaaag ttttctgata acttcttatc aacattttct gatagcttga
     2521 aatcaggtta ttatcgatat caagcaaatc tttgggggat ttcgattggg ttgacagcgt
     2581 tggcaatcgc ctatgtgttc agcgcgtttg tgatcattac agcaatatta gaacttgcat
     2641 ttaaacgtgt gttaggtgtt cttgtttttg cgacagatat tgaaacagga caacgttcaa
     2701 aagttgtttt atccgatatt ttacaatgtt acttaacagt tggatttcaa gggtttggac
     2761 tatctatgtt tgctatgttt atcaattttt taaatgctgg acaaggtatc tccacaaata
     2821 tttttataaa aacaattgct tatatctgtg ctgtgtttgt gctgataaaa ggcagtggaa
     2881 cagtcatgag atactttggt gttgatattg gactcaaaga aggctatggg caacttgctt
     2941 ctgcttttgg tatgggcgca atgctgttta gaaaaggatc aactggattt aatcgtgcta
     3001 aaggaagcgg aaatggaaat ggatcagaca gtggagaagg agaagatcga aaaccagaaa
     3061 agaatttcgg cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg
     3121 cccatgaacg tggattatca ggtttagctt cagatggagc tacaatggct agtgaaagag
     3181 caataaaacc atttaagagt atgcgtgata tggctaatga taccaagaat aagttcaaag
     3241 aaggtttaga tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct atgttagcta
     3301 aaaataaaga agatgaaact ggaaaatatg ctgattccat gccaacacgt ctttcggata
     3361 gaaaagatgg tgctaaggtt gaaaatgccg atagaattat gtctagttct gaacgtatgc
     3421 gtgaagcaat gaaaaataat gctgaatcga ataataatcc tgtaagtgca attcagcaaa
     3481 aagttcaaca agatattgaa gaacgtaaaa atgctatgca cggtcaagct aagtctgctg
     3541 aagaattaat taatcagaag cgacaagaag ctaaatacac gcctgaagca atgaatcgtg
     3601 aggaaatgtt aaggaaacgt gtagaagggc gtacaggagc taatacggac gaaaaagaag
     3661 cgttgaccaa agaaagaatc caagaagcta aaaactcaaa taccggatta gaaaaattgg
     3721 tcaaagaaaa tctacaaaat tctacaagta gtcaaggtgg ccgatcagta gatgtccgag
     3781 aaaatattca aggttcattg aatggtcatg tcggacggaa tgttgatgta agagagaatt
     3841 tacaaagctc ttcaaatggt caaggtacta agacaattga tgttcgagaa aatgtacaaa
     3901 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaaattcta
     3961 caagtagtca agggtttaaa tcaatggatg taagagagaa tgtacaaagt tctacaagta
     4021 gtcaaggatt caaaacagta gatgttcggg agaacgttca aaaagatagt ggagaaatga
     4081 aagaaaaaac acaaaaaatc aatatcgttg aagacagaaa gtcagcgaaa aaatttgatt
     4141 caaatcatga aacagtgatt attgatagtg aaatccgtga aaatgataag ggatcaaaac
     4201 caagacgaag atttacctat gaagacaacg agttattcag agatacactt aatgatccta
     4261 atccgctttt cgataatcta ttaaaaaaat aaaaggggga attgtcatga aattaattct
     4321 aaagattagt acgcatgatt tcttagaact tgacgaatta tgtgaatcag taaagaacgc
     4381 aacaaatgaa aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa
     4441 gatgaaacat gagataaaaa acaacttgaa atattgttat ctagaaaaaa gtgaagagat
     4501 aatccatgaa ttaaatggct tttaaaaatt gtatttgaca tgaatttacg acaaggaatt
     4561 agtggggatt aagcgattat tctcttccac tttagagaaa taatgggtta aaaagccaga
     4621 atacgtagag caatattgac aacctataaa ggagctattg ttgaaaattg aagtaacaaa
     4681 aagataaagg gaggtttaca tctatcagat taaaagtgat agtacaccca tgaaacctgc
     4741 aaaagagttg aacctaatgt atatcggttt aactcttttt atttgcaaat taaaaaaata
     4801 tcaccgaagt gacgaatgaa actatgacca aattaagcaa gaaaatatat cttgcaaata
     4861 aagtagtata aaaagatatt gataaagcca atcaaaactt tgaatttcaa acgttagata
     4921 atcaaacaga tacaaaaaat gaaatcagtt ctacgcgtac gattattaaa aataataaat
     4981 tttcactaga agaaaatgga aataaaatca aataattagg aagaaaaaac aaagcaatta
     5041 gaaaagcctg caagcaaatt tgagtaatcg agatccaatt tttttaagga agtttaacaa
     5101 aaatatgtaa ttctattatc actattaaag agaaagttgg agcggaatat aaagaaaaga
     5161 gaaaagtaat taaacgcaaa aaaatgtaga acttaaaaaa tataaagagg aacaaccgtt
     5221 aaaattagga atgatgttgt gtaaacatta taattgctat gatataaatg aagtgttaaa
     5281 gaaagcaaat ttaaaccaat tttgtgaaca gaaaagcctt agtcactatg accaaaacta
     5341 cggacgaaac tatgaccaaa agtaggtact aaattatgac cgaaaatagg tccaaaatgg
     5401 atttttttat gatatcaaaa attatgaaat gattttgaaa gaaaaccagt tggcacagcc
     5461 aacttagcca ccttcctgta ggaaggtgct atcagttttc ccttatgctc ttttctttct
     5521 tttttgtttc atatgtcaaa tgccattttg aaatgtacca tcttaagaaa tttatcctta
     5581 aaaacaattg cgttattgga taaaaagatg ctacattatc ttaaaaaagg aatcaaatat
     5641 gatgaattta gtggctagca tatcgtgtga ttaagatact ttatagtgta agttgaagga
     5701 gaaaaaatat ttaaaatata cttcccattt cagtgggaag taaaattaat cttgttgttt
     5761 ctactagtaa tgtatctttt tggaaactat gtaacgatgt aaacaagatt tttttcagaa
     5821 gaaaaaacga taaaagttgt gataaacgat ttaaaaaata ggagaaaata tatcaagcgc
     5881 atggtatgaa acgcacattt aaaaagattc gactaactaa aaaaataagt ttacaaagca
     5941 ttgttgtgta ttacaagtga tctatacact gaaaaatgat agagaaaatc tgatacgtcc
     6001 gcaacaatgg acaattgaaa atgaaagtga ggaaattgga agaatggctg atgaaaataa
     6061 agttgttccg cttgaaacac tagtgataaa taaggaagag tttgagcaac tttttcttga
     6121 tttttttgtt caatatgaca ggatttatga ttttagttcg tcactagatt tatttgaatt
     6181 agaggcagaa ataatagaaa acagtcgaga ggagaatggt aaaatcatca tttcagtaga
     6241 aaaattcaat gaattattct cagaaacatt tgaaatagat acagattatt atcaaccaca
     6301 agaagttttc caatcattta gaaatcagat tattcgctca ggtaagaccg taaactgaaa
     6361 aacaataaaa gaaacgttga aagaataatt attccttcaa cgtttctttt ttagaatgtt
     6421 ttaatgacca aagcagtttc attgtcaggc ttaggtaatt catgaaatcc cgtatttata
     6481 gtgctcaatt gatcaactaa ccatgtttta ctttttgaat tgtagataaa tctaaaatta
     6541 atatcgttat tatcgtgttg aaggggcgtc tccaaataat ctgctgtata ttcatagtca
     6601 acgtaacctg ctaatacaaa agagagtatc cctttattta atgttagatt taatggataa
     6661 tcatacgact ttattttttt caacttcata ttgtacatat cttcagccat aaaagttgag
     6721 ttcgtgattt catcttgggt ctcattaaaa aagttatccg aataatcgct tatttttgaa
     6781 aagtctttat tggcaccagc ctcaaaaaat tgttcaactt tttcagtata aagttgtgct
     6841 aacgctcctt tttcaatatt aactttaata ttttcatgtc tatcaatttg tgtgctgttc
     6901 agtaagggaa gatcagcttt tagttttata tcgtcttgat ttctttcaat ttcaattggc
     6961 aaaatctttt cagtagattc agtttctgat aaaggaacat ctagtttttt atcatgataa
     7021 taagcttcaa atccatctgg aaccggaaaa aaatgtattg ggtaattctt ttctgaggtt
     7081 gttgtactag tttccgtact tgtagaactt gtgtcagaag tggtacaccc gcttatagtt
     7141 ccgatgagta aagcgcaagt tagaatgatt gcaaatttct tcaaaaaaat tcctccttaa
     7201 aattgaatat ggactaatac tagtcccatt gtagcatacc gaatctttaa gattaataag
     7261 aaaaaggact aattttagtc caaaaggtgt gctattcttg aaagaggaaa aaattattga
     7321 taaagaaaat gtaatcggta gcaatattag acgagtccga ttagaaaaag gaattggtca
     7381 aaccgagctg attagagacc tacagcttcg taaaattgct ataactaggg aaacgttggt
     7441 taagattgaa ggtggccgcc aacacattaa gttggatcaa ttgaaagcca tcaaagatat
     7501 tctacaggtg tcttatgaag atttgcttca atgactactt gtagtcattt cgtttgaaat
     7561 tattatgtta cacccttatg tgtcacgaag aaatactggt ataataatga atgggatatg
     7621 taacgatagg gtatactgtt atgttgccta ataaattgtg gatcaaaata caagaatgga
     7681 gggatatcct tgtataaggc atatgaattg gattttacaa atgctgactt aaatgcattc
     7741 tcctcaagct cgcaatcata tacacatgta gtaaatcaaa tagatcacaa ccaaaaatct
     7801 attaacaaga gaata
//
LOCUS       DAYYKC010000116         7806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000116 DAYYKC010000000
VERSION     DAYYKC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.118"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHBBW_002624"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHBBW_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9154254.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="ACHBBW_002625"
     CDS             complement(1378..1896)
                     /locus_tag="ACHBBW_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154255.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="ACHBBW_002626"
     CDS             complement(1912..2238)
                     /locus_tag="ACHBBW_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9154256.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="ACHBBW_002627"
     CDS             complement(2251..4230)
                     /locus_tag="ACHBBW_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9154257.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="ACHBBW_002628"
     CDS             complement(4251..4565)
                     /locus_tag="ACHBBW_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9154258.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="ACHBBW_002629"
     CDS             4733..5026
                     /locus_tag="ACHBBW_002629"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9154259.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="ACHBBW_002630"
     CDS             complement(5104..6216)
                     /locus_tag="ACHBBW_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9154260.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..7217
                     /locus_tag="ACHBBW_002631"
                     /pseudo
     CDS             6369..7217
                     /locus_tag="ACHBBW_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7570..>7806)
                     /locus_tag="ACHBBW_002632"
     CDS             complement(7570..>7806)
                     /locus_tag="ACHBBW_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9154261.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2495 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatc
//
LOCUS       DAYYKC010000117         7600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000117 DAYYKC010000000
VERSION     DAYYKC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.119"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHBBW_002633"
     CDS             complement(138..428)
                     /locus_tag="ACHBBW_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154262.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHBBW_002634"
     CDS             complement(546..806)
                     /locus_tag="ACHBBW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154263.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHBBW_002635"
     CDS             complement(943..1356)
                     /locus_tag="ACHBBW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9154264.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHBBW_002636"
     CDS             complement(1812..2297)
                     /locus_tag="ACHBBW_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154265.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHBBW_002637"
     CDS             complement(2436..2651)
                     /locus_tag="ACHBBW_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9154266.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHBBW_002638"
     CDS             2875..3675
                     /locus_tag="ACHBBW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9154267.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHBBW_002639"
     CDS             3694..5562
                     /locus_tag="ACHBBW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9154268.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHBBW_002640"
     CDS             5555..6046
                     /locus_tag="ACHBBW_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9154269.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHBBW_002641"
     CDS             6034..6588
                     /locus_tag="ACHBBW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9154270.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACHBBW_002642"
     CDS             6606..>7600
                     /locus_tag="ACHBBW_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9154271.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAYYKC010000118         7558 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000118 DAYYKC010000000
VERSION     DAYYKC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.120"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..1249
                     /locus_tag="ACHBBW_002643"
     CDS             257..1249
                     /locus_tag="ACHBBW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154272.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1265..2149
                     /locus_tag="ACHBBW_002644"
     CDS             1265..2149
                     /locus_tag="ACHBBW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9154273.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2198..3805
                     /locus_tag="ACHBBW_002645"
     CDS             2198..3805
                     /locus_tag="ACHBBW_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9154274.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3806..5290
                     /locus_tag="ACHBBW_002646"
     CDS             3806..5290
                     /locus_tag="ACHBBW_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9154275.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5467..6459
                     /locus_tag="ACHBBW_002647"
     CDS             5467..6459
                     /locus_tag="ACHBBW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9154276.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6511..7380
                     /locus_tag="ACHBBW_002648"
     CDS             6511..7380
                     /locus_tag="ACHBBW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9154277.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2420 a         1206 c         1659 g         2273 t
ORIGIN      
        1 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
       61 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
      121 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
      181 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
      241 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
      301 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
      361 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
      421 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
      481 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
      541 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
      601 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
      661 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
      721 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
      781 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
      841 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
      901 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
      961 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     1021 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     1081 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     1141 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     1201 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     1261 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     1321 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     1381 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     1441 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     1501 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     1561 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     1621 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     1681 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     1741 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     1801 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     1861 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     1921 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     1981 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     2041 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     2101 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     2161 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     2221 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     2281 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     2341 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     2401 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     2461 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     2521 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     2581 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     2641 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     2701 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     2761 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     2821 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     2881 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     2941 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     3001 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     3061 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     3121 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     3181 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     3241 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
     3301 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
     3361 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
     3421 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
     3481 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
     3541 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
     3601 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
     3661 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
     3721 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
     3781 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
     3841 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
     3901 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
     3961 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
     4021 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
     4081 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
     4141 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
     4201 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
     4261 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
     4321 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
     4381 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
     4441 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
     4501 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
     4561 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
     4621 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
     4681 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
     4741 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
     4801 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
     4861 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
     4921 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
     4981 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
     5041 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
     5101 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
     5161 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
     5221 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
     5281 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
     5341 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
     5401 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
     5461 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
     5521 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
     5581 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
     5641 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
     5701 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
     5761 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
     5821 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
     5881 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
     5941 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
     6001 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
     6061 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
     6121 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
     6181 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
     6241 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
     6301 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
     6361 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
     6421 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
     6481 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
     6541 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
     6601 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
     6661 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
     6721 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
     6781 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
     6841 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
     6901 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
     6961 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
     7021 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
     7081 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
     7141 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
     7201 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
     7261 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
     7321 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
     7381 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
     7441 taaatagtct caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt
     7501 caaattaata taattacgaa atgatattga gtacaagaaa gttcttttta ccagatct
//
LOCUS       DAYYKC010000119         7486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000119 DAYYKC010000000
VERSION     DAYYKC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.121"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBBW_002649"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBBW_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9154278.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBBW_002650"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBBW_002650"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9154279.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHBBW_002651"
     CDS             complement(2298..2960)
                     /locus_tag="ACHBBW_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9154280.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHBBW_002652"
     CDS             3144..4790
                     /locus_tag="ACHBBW_002652"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9154281.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002653"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBBW_002653"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9154282.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7486)
                     /locus_tag="ACHBBW_002654"
                     /pseudo
     CDS             complement(5971..7486)
                     /locus_tag="ACHBBW_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2348 a         1368 c         1383 g         2387 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttc
//
LOCUS       DAYYKC010000120         7331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000120 DAYYKC010000000
VERSION     DAYYKC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.122"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(401..2036)
                     /locus_tag="ACHBBW_002655"
                     /pseudo
     CDS             complement(401..2036)
                     /locus_tag="ACHBBW_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2475..3344)
                     /gene="scrK"
                     /locus_tag="ACHBBW_002656"
     CDS             complement(2475..3344)
                     /gene="scrK"
                     /locus_tag="ACHBBW_002656"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9154283.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3364..4485)
                     /locus_tag="ACHBBW_002657"
     CDS             complement(3364..4485)
                     /locus_tag="ACHBBW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9154284.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4487..4882)
                     /locus_tag="ACHBBW_002658"
     CDS             complement(4487..4882)
                     /locus_tag="ACHBBW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9154285.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4901..5698)
                     /locus_tag="ACHBBW_002659"
     CDS             complement(4901..5698)
                     /locus_tag="ACHBBW_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9154286.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5700..6488)
                     /locus_tag="ACHBBW_002660"
     CDS             complement(5700..6488)
                     /locus_tag="ACHBBW_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9154287.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6502..6978)
                     /locus_tag="ACHBBW_002661"
     CDS             complement(6502..6978)
                     /locus_tag="ACHBBW_002661"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9154288.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7050..>7331)
                     /locus_tag="ACHBBW_002662"
     CDS             complement(7050..>7331)
                     /locus_tag="ACHBBW_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9154289.1"
                     /translation="ERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLE
                     DEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         2364 a         1505 c         1086 g         2376 t
ORIGIN      
        1 acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa accactcatt
       61 ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa
      121 gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc aaagataatt
      181 ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca acatagcgtt
      241 gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc caattctcta
      301 taaagaagat taagcttcat tattataata taaagtttaa atttggttta ttacttactt
      361 tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt atttttatat
      421 gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat aaactaatta
      481 aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt gtttctcctt
      541 gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc atttactttt
      601 gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt agattcatgt
      661 actgtataga ctattttcga atccatcact tgataaggca tagtatgcca ttccttgcca
      721 attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt gggtgcatag
      781 tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg attcattacc
      841 aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc agcagataca
      901 gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt agctgatgtg
      961 ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa gcctaagatt
     1021 cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca tggaaaaaat
     1081 ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc tttgcgatta
     1141 aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat tcctaccgta
     1201 aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt taatgcttgg
     1261 tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg tcggaacatc
     1321 ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga caactccaca
     1381 atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc taagactagt
     1441 aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat gagtaagctc
     1501 atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc gtaagcataa
     1561 tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga acgatgataa
     1621 atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa agccgcatcg
     1681 attcttttta aaaattttag tccataatga aacatcagca tgatagtaca aacattcaat
     1741 agccaaacat ataagatata actaacgatt aaattattac ttatccaata aaaaatgact
     1801 gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata atgattaaac
     1861 gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct agcaaaatgg
     1921 aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt gattgaataa
     1981 agtccagcaa taattgctag gagaatggac aatattattt ttttctttac ttgcattact
     2041 taattatata ctcctttcat ggattgcttt atctagtata acttaaattt taaataaatc
     2101 gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg gaggtaatgg
     2161 tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc aaagcttaat
     2221 tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt
     2281 tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac tactttacta
     2341 tctttcattc ttcttgtctc acttatgata aggcgctggg tatcatgagt gaatgatatt
     2401 gctgaataca aatagtccga ccacattaag ttgatcggac ttcataagca ttttattgac
     2461 taatactttt ttgcttaaga attgattcta acatcttttg ctaaagttaa gcatcctata
     2521 gttcctgcat tatctgctaa cttaggtgtg acaatgtatt cttcgatatt tggcgtatcg
     2581 acatagccgt taattagttc taaaaaacgt ctttgtactt tctttttcag atgttcttgt
     2641 tgcatgacac caccaccaaa aattataata tctggtgaaa gcatcagggt cgtattacag
     2701 gcacattgtg caatataatc cgcctcgatt tcccagaacg gatcttctat agaaagatct
     2761 tgtcccttgg ttcccgtacg catctcgata gcgggtccag atgccaaccc ttcgagacaa
     2821 tcatgatgaa aaggacaagt accagagaaa gtatctcctg aatgtctctt aactaacgta
     2881 tgccccattt ctggatggct aaacccttca acaaaaatcc catcttgtat agctcctcca
     2941 ccaattccag ttccaacagt gtaataaaca acactggata aaccttttcc atgacctgaa
     3001 acatactccc cgtaacaagc agcattcaca tcggttgtcc aggcaattgg aatttcgaaa
     3061 tgcttattca acgttccaac aaaatcaaaa tttttccagg cagtttttgg agtagaggtg
     3121 atatgtccat aggtctttga ttcacgatgg atatcaatcg gtccaaatga ccctactcca
     3181 atgctttcta attgttcttt atagtgatta aagaaatcaa ttaccaaact cattgtttca
     3241 ttcggtgtag ttgtaggaaa actcactctt tctaaaacct ttaaatcatc attgccaatg
     3301 gcacaaacaa atttagtccc acctgcttct attgagccat acatttctta ctccaccgtc
     3361 cttttatcta ggaattcgtt cttccctgtc taatagtttt tcgaaaaact catgtggttc
     3421 cttttggtac caataggcaa cagaagaaat atcgtcttga cgctcataaa gcttgatgtc
     3481 atcatttcct agggtttgga aagtaacacg taaattttct ttgaaactaa tggggtcggg
     3541 taaatgccag cggtacaacc catgcatcgg taaagcatca tcgccaaaac catgaactgg
     3601 atttggatta ggttttccag cactaaaata atcccgggtg tgatctcttt tagattgaaa
     3661 aggatagccc ataaatagcg tattaaaatt ttgagcatat gccctttctc caaagttctt
     3721 tttatggaaa gcccaagcgc cgccaaaata atcttctgct cctgtagatg ttattgtagg
     3781 gaaatcttca tctccatcta tataaaactt aaattctcct tccccccacc agtatctttc
     3841 caatgcgcac aaagcaaagt aggttcctac ataataccca gtgcctttga ttccatcaat
     3901 aagggtgtaa tcttttcctt ttgtcgtgat gggttcacgg ttccatttag catgaaaata
     3961 catcgcatca tccggtactt cagtcaatgc ataattaaat gtataaaaaa tgtgcttaat
     4021 aaactcagga tgttcattcg ttaaagtaat cttagctttt tttcgaaatg gcatctcaaa
     4081 atagcaattc atccctgatg ttggattcac tacgattggt aaggaattca cttcgcatct
     4141 tgtcccaaac ccattacaaa aaaaatctcc taatggactt tctacagaag gagtggattc
     4201 atcatcccaa taaatacgca aaattaaatc acgtaagacg aaactcccag cgattgtttt
     4261 gtctgtcact gtcatccaaa aatgacgtaa aattccaggg ccttccattt ctgctaaagt
     4321 aattgtctct ccagcattta tcggaacaga aggtgaccct tttctcccaa ttcctagatg
     4381 actcgctgtc attgccgctt tccctttttc accattttta ttttctggtg aaatagttct
     4441 actttcctct cccttgtgga gccatatatt atttaaacaa ttcattttat tcgtcctcta
     4501 tcctttcttc acttttttga ttattcatat agatgatttg ttgttgtgcc tccgcaataa
     4561 ttgctccaat atctaaatta tcatttccta actgcaaagc gattgacatt gccaagggaa
     4621 gattcattcc tgcaattacg tgcactctgt cagaaatata ggtaaaaaac ttttgattta
     4681 cacttcctcc agccatgtca gttaaaacaa ttatttcgtc tgtagtatcc actaactctt
     4741 taatttgttc atctatatca tcaatttctt cgacatatgc acataagcta ctaatttgat
     4801 tatgcatacc tgttaaaaac tctagtgtct ctttaaaacc ctcagctagt attgaatggc
     4861 tagcaatgat caatcttctc ataattcaaa tccttttcct ttacgctaat attccaaatg
     4921 cagccataac catcgcaaaa acaataacga gtagaatcaa aatggtcata ctgatttttt
     4981 tcttactgag tagataatat gctgcagcag taataattac tggaattaat gttggtaaaa
     5041 ttttgtccaa cattggttgg attttcatac taacttcacc aaaagtgaat ttaagtggcg
     5101 ttgaaacact gataactgaa gcaattaatg atccaactac tgttagcccc aatactgaag
     5161 cagcttcagt taaataatta atcttttcac cgtattcagt aacaatcttc attccctgct
     5221 tataaccaaa ttcaaagcct ctcattcgga aaaagaagat taccatgatt aaaatgaacc
     5281 ataaaaagat tcctacagga ttattttcta aagccatata agccgcaatt gatcccatga
     5341 tggttgggat catcgtcatc aaaagagagt ccccaatacc agcaattgga cccatcgtac
     5401 caactttaaa gtcattaatc gcatcttttg cttctaatcc ttgcttttcc tccattgcaa
     5461 cacaagcacc taaaataatg gctgccagcc aaggctgtgt attgtaatac tgaaagtgat
     5521 tatttaaagc ttcgatgtat tcatcatcat ctttgtataa ttttcttaaa ataggtgaca
     5581 atgcgtaagt cacagatgca ccttgctgtg tttgataatt aaaggtacaa actgacatca
     5641 accaacgata gttcgctttc ctaagatcct ttttcgttac ttgatatttt tcgctcattt
     5701 tattcatcct ccccaataaa cccaacatca gaagctcccg cagaagcagt gatttgtgca
     5761 tctttttcag ctctatgata cacattaaat gctaaagcga atcctatgat cgccgctcca
     5821 ataataggta ccttcaaaaa agcggctaag aaaaatccaa caagtaaata actaatgtat
     5881 cgacctgtag gcataaagcg taacaacatc gcaataccaa cagcaggcaa catttttccg
     5941 gctaatgtta gcccatccat aaaccaatta ggtaaaacat ccaaaacgta attaacagca
     6001 gactgtccta aagtaacggc aataaatact ggaattgctg cagataaacc aaagaaaatt
     6061 ggccccaacc acaaaacact attcattttc ttaaattctc gtttattcgc atattcctgt
     6121 gatttgcgaa caacaaaacc atttaagatt ttgacaatta catctagttg cacccctaac
     6181 atacctacag gtagtcctat tgctaatccg gcttctactc caagctggct accaaatgcc
     6241 gttgcaataa ttgcgcacgt tccatagtcc ggtacagatg agccaccaaa accggcaact
     6301 cctaatgaca tcaattgaac tgtaccgcca actatcaaac ctgtttttat atcacccatc
     6361 aaaattcctg tgaataatcc ccaaaaaact gcgtaattta caaagatcat cggaatccca
     6421 taataatcaa catgtttaat aaatgctaat atagtcagca taataatttg aaaaatagta
     6481 aaatgcatta tccttactcc tttatcctaa ttttgattga atttcctgtg ccttatccgc
     6541 cggaacaaat tgtgtaacaa tttctacacc agaatccaac aattttttat acgtgctgat
     6601 attttcatct gatgcatagg ctctagaaga tagctgtaat gcattatctt taacaacagt
     6661 accgccaaca attagtcggt caattctaat accgttatct actaaatgaa ggattgtttc
     6721 cggctctttc acgatgacaa atactttttc ctgatcgtat ttcccctttt taaaattcgc
     6781 aatcgctgtt tcttcagtga taattgatac agccattcca gcaggtctag ctaaacgcat
     6841 acttgatttt tggatgtcgt tatttgcgac agaatcatcg atcaccatca ctctgtttgc
     6901 tttagacatt ggtgcccatt gcgtgacaat aatcccatgc aataaacggt tatcaactct
     6961 cgacataaca atactcataa atacttcctc ctttatcttt cattttctta caagcaatta
     7021 ccatgccaaa acaaaaagaa cttgataaat caagctcttt ttacataatc ataaacgtat
     7081 ttaacctcat gaatcgggat gtcgacacta taataatgtt caacgacact gaaagattgc
     7141 ttaaaatgtg tctgaaattt ttgaaattcc tcactcgttt cattgaaata aatttcatct
     7201 tccaaagtat tttgttttac taatcgttca attaagcaac taatgtgaac atataatccg
     7261 aaacacgtat tattatcaaa tgaaattcct agatcatttt gtaagatgta tatagcatct
     7321 gaaacccgtt c
//
LOCUS       DAYYKC010000121         7280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000121 DAYYKC010000000
VERSION     DAYYKC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.123"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            440..1600
                     /locus_tag="ACHBBW_002663"
     CDS             440..1600
                     /locus_tag="ACHBBW_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9154290.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1635..2402
                     /locus_tag="ACHBBW_002664"
     CDS             1635..2402
                     /locus_tag="ACHBBW_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9154291.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            2593..2991
                     /locus_tag="ACHBBW_002665"
     CDS             2593..2991
                     /locus_tag="ACHBBW_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM9154292.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3499..3672)
                     /locus_tag="ACHBBW_002666"
     CDS             complement(3499..3672)
                     /locus_tag="ACHBBW_002666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154293.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFL"
     gene            complement(3694..4044)
                     /locus_tag="ACHBBW_002667"
     CDS             complement(3694..4044)
                     /locus_tag="ACHBBW_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154294.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(4061..4456)
                     /locus_tag="ACHBBW_002668"
     CDS             complement(4061..4456)
                     /locus_tag="ACHBBW_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM9154295.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            complement(4523..4708)
                     /locus_tag="ACHBBW_002669"
     CDS             complement(4523..4708)
                     /locus_tag="ACHBBW_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM9154296.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4790..5044)
                     /locus_tag="ACHBBW_002670"
     CDS             complement(4790..5044)
                     /locus_tag="ACHBBW_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154297.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5318..5602)
                     /locus_tag="ACHBBW_002671"
     CDS             complement(5318..5602)
                     /locus_tag="ACHBBW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154298.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(6096..6581)
                     /locus_tag="ACHBBW_002672"
     CDS             complement(6096..6581)
                     /locus_tag="ACHBBW_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154299.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKQQLDRIKEE
                     NNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(6748..7014)
                     /locus_tag="ACHBBW_002673"
     CDS             complement(6748..7014)
                     /locus_tag="ACHBBW_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9154300.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(7016..7237)
                     /gene="relB"
                     /locus_tag="ACHBBW_002674"
     CDS             complement(7016..7237)
                     /gene="relB"
                     /locus_tag="ACHBBW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9154301.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
BASE COUNT         2408 a         1171 c         1216 g         2485 t
ORIGIN      
        1 ttaaatagat tttagggtgg acataaagcg atgcgaacat ggacaaaaag cgatgtgaat
       61 gtggacaaaa agcgatgcaa acgtggacaa aaagcgatgt ggtattccga atatatccaa
      121 aaaaagaaaa agtatgtgaa tttatttcta aaaactagaa aaatatttgt tttttaattt
      181 tggtaatttt tataatttcg tattttattg aaaaggattt ttattttgaa aatgttctaa
      241 aatagcgatt tctgctgtat tttcaatgaa atttgattgg aaaaaataaa atgtgtttta
      301 ctgacattga gttatgttat aaatttacga tacatttctt attgtaacgt atcgaaaaca
      361 ggtctatact tattcaatgg aatcgtacca gttccaagtt tttaagctcg taccaagctt
      421 agaaagaaga ggcaatcgta tgaaaataac agaaaagaat gtaaacccga aatttgaaac
      481 taccgaaagc aatctaaaaa agtttgattt aactgttact ttatcgaatc aacttgtaga
      541 tagcattgtt gaaatggatg ctatgccaca aaaaatttat ctttacgcta taggacatct
      601 tgaccctaaa aatccgcctt ttaataatac cgtcaaagta tctaaaaagg ctttatttgc
      661 gttgatgtct tctagttcat cttcttcaaa acattcaaga ttaaaacaag ttattgaaaa
      721 attgcatcag caatctgtca ttaaaattca aagaactaaa cctaacggta agactgaata
      781 caaagtcatc tcaccgctag aaattactga attcaatgat ttttctgatg cagtatctat
      841 taaatttagt agttcaatta tgcctttatt aacgaactta gaagatcatt ttacgcaata
      901 tacatttgat caagtagtta acttgacaag caagtatgcg atcagttttt ttcaattatt
      961 cactaaagat tttaatctgt atgaagcaaa tagagataac ttaacatgga gtacaaaatc
     1021 acttgataga ttaaaaaatc ctgttttctc cattgcagat ttacgaaaga aatttagtat
     1081 tgagaaaaaa tataaagctt ttcgagactt tgaaagacgt gtaattaaag aaccgattgc
     1141 ccaactgaat aaatacactg attttaaaat tgactatgag cttatcaaga agggtcatac
     1201 ttatacaaac ataaagttct tgcttacacg taccatgtca gttccggtac cttataagca
     1261 agaacaaaga gaccctgctt atttaaaagg cgcagaagat aaaacagaga aaatgaatca
     1321 atcgtacttg aaagctatga atagcaaata cactaaatta ctaatcaaaa actttttgat
     1381 cagttctgcc gacttatgcg atcttaaact tatgagccga cttcagaata aagtttatcc
     1441 gctttatgat cgtttatcca aagatagagg atttgaatca gttgtcaatc acctatctta
     1501 cgttgctact cataaggagg attacacaat aaaaaatact gttaaatatc tcgaaaaatc
     1561 aattacgagt tatttgcctt atgtgaacct ttatgattaa caactgaaaa aatgcgtgaa
     1621 ggaaggaaaa acttttgaaa aaagatgaac tgacaaagta caatgaggag ttcttagcaa
     1681 caattgacat gaaaatggat ttaaagtcgt atgaacttta tcaattgctg ttcagttata
     1741 tctctcctaa caaattaaag aaaacagggg aaatagtttt acctgttaaa gcaataatca
     1801 aatacatgga tgttgatcaa aatcttgaaa cagaactaaa aaatatagtt aacacccttc
     1861 agagacaagc agtttatgaa atatcgaact tagataaaga atataagact tatgctacag
     1921 tatctgctat tgatcaaatt cattgggact tagaagaaca aattataact ttcaatttct
     1981 cgcaagaagt aattccccta attctttcaa tcaaaaagaa atatccatat tttgtttttc
     2041 aagatttagc aagctttcaa aaaaaatata gcattgtgtt atacaaatat ttggcgatgt
     2101 attataaaaa atatcttatc gccaaaaata aaggagattt tgagaaagct gaaatatata
     2161 agaatcccct gattgaaata gttaaaattc gtgagattac gaaaacagaa aatgagtaca
     2221 aaacaatatc gaattttgat agacgtgttt taagggaatc agttgaggaa attaatgaaa
     2281 aatcaatgct gaagattgat tttgctaaag ttagaaaagg cagaaattac aattttgctg
     2341 tccagtttaa tatttctagt gagtttgatg aaaaacctta tagagaaaaa aaattaggtt
     2401 aatgcaaaat atatcttact cgtttgaaga tgcttcagac gtatgctaag atatgtagtg
     2461 ccttgggagc aaggtattaa tctcctttag aggttaatta gcttgtgatc gcctatcaat
     2521 ttttttggta ggcgattctt tttttgaaac taaaaagcaa aaattatttt ttaattagag
     2581 aggtgcaatg cgatgaaata ctttttttgt gcagtattcg aatggaaagg aaatgaatat
     2641 gcagttaaat tcccagattt tccagactta tatacgcatg gtgaaacttt agaagatgct
     2701 tttgagatgg cgcaagaagt attggaagga tacttggtgc ttttagagtt gcaagaaaaa
     2761 gaaattccat cgcctacgcc tgttcaagag ttaaatgcaa gaatcaagcg atctgagaat
     2821 gttaatttat ttgtcgtaaa agctaatacg aaagttgcta aagctcgaaa caagcaaaat
     2881 tacgatcgaa aatcagtgac tgtggaacac tatttaaatg ttttagcggc tgaagagaat
     2941 attaatctgt cgaaagcttt gaatgatttg ttgagagaaa agttgatttg atgtctctca
     3001 gatgatcata ttaagcaaaa acagcttgtt ttaggacgta tggatttaag aaagacaaat
     3061 tatcaaagaa tatattaaaa gctctcagaa gcgttctgag agcttttaaa ttgctatgta
     3121 ttatactgca tctgctcaaa ttgattttct tttcatgctt accatgtatc tagttttcca
     3181 tcaagtaatt tttagatatg tattgaggaa taaatagcaa atttgcttga tataagctcg
     3241 aattaggtgc tttgtaatgt gtttggataa attatacaag aaaatgtcaa aagctctcag
     3301 aacgcttctg agagcttttt atcggtaggc ttgtttaacg tgatcttgaa agataccctt
     3361 ttgatgtttc tctcgatccc tgcatcgcta attactaagt gttcttcaag tgtgatgtag
     3421 gtatataaat taagcatcaa aatggtgtag ataagcaaaa aactccctaa aactaagtgt
     3481 tgattataac aatctgactt ataaaaaaag aactgagaat gttagtgttg cttcccaaaa
     3541 aagaaacaaa attttgtatt tccaaagata gtggatcaag cgctgtgctt tactcatctc
     3601 aaataattta taatatcctg ctaattgatc agcaagcgta tcgttaatgg gttctgtttg
     3661 tgtttgtttc atctttctct tctcctgttt caattaatct aaaatttcag attgagcaag
     3721 caactcattg tggtagtcgg tttgaaattc aaataacaat tttgccttct taacgaggtt
     3781 taaagcgttc gttagtgatt cacttaatgc ttcaacatct ttaactgatt ttgcatgttt
     3841 cattttttca gtgtgcgtat agcagaattc aggtattgag ttaggggttg cctgttcaat
     3901 aaacatctca attcgcaagt tattatcctc aaaaataatt agatcattgg caaagtgttt
     3961 ggtactaacg ctaatgccag ttgtatcttt taatagatta ttgattaacg ttcgagagaa
     4021 atagtttaag tgtgtagctt tcataggttc ggtctccttt ttacttttca aacaactctt
     4081 cttctagtgc tttttgaagt agttgagaga agtttatgtt tttctccttc cctaaaagaa
     4141 ctaaccattc cggaacacta acattttttc taattgtttt tgtatttaag cttcttcgga
     4201 aaagtttaat gtttagttgg atgattacta ccacttgatt ggctttcaaa gttaattctt
     4261 ccactgctga tggtttagga aattctgcat ctggtacact atatagcgct aatcctaaag
     4321 catccttagc gtttaaatat gcttcttcaa gactatttcc aaaggtagat atttccggta
     4381 agtcagggaa aacgatgtta taagcttctc cttctttctc gaagattgcc ggataaaaaa
     4441 tattttttgt cttcatttcc tcgctccttt tctagtgtaa gagaggatag tatgcctaat
     4501 accaaatagg aaggcaaggc tgttaaagcc ctgcttgctt ccagatatta gcttctgttc
     4561 ctagtggtac atcaccattg tggatcggaa caacgaccgt tcttttagtc ttggaattat
     4621 aaaattttgc atgactgcca gtttgtccag tctttttgaa gccgttagat ttgagaattt
     4681 ttacaatttc tcttgctgtc ttaggcatag ctaccctccc tttacaaaat ctattataca
     4741 cattgttgtg tatggaatca atagaaaaag aggatgtttt attgcaaaac taataaaatg
     4801 atttattggt tttttcagca aattgaatgt tattagcgtt gcaaatattc ataatttccc
     4861 aagtatcatt agaagtatag gtatctaaga attcattgac agttacggtt gattcgtatg
     4921 gttcataagt caaagcgttc tccaagtctt ttgatgaaat gatcaagact ttttcaattt
     4981 cctccccttt ttcatttgtg aaaacgtatt ccaaagtgat ttgttcttca tcggtataat
     5041 tcatattgtg ttctcctttc ttgtattaag aaaaatggat atttccaatt gcatcccact
     5101 cggcaccatt gtggtataag acgtgaccga gcgttacgcc tttgaaatgc tcttctaaat
     5161 acatctaaac gcattcaagg ctagtacctg ctttgagatt gtagaagtct gcatccaata
     5221 ccttttcaga agttatcgga tcaatgcgag ttggtacctc aataaattca gtccaaattt
     5281 ctgcaaaagt cttaccttcc aaagtccaca ctccctatca attaattcca aaagcaaata
     5341 gagaaatcaa gcgaccgtca atagtcactt caacaactga aaatgccgtt gaaacagttt
     5401 taacatccac actttccaat ttccctatga aggaattttt gtgctttaga atagctttct
     5461 ttaactgtct gttatacgct tgacgactgt taaacttttt gaattctcga tccttgatca
     5521 gctgtttaac gacccctgca ttaattgtat agctttctgc aatagcaacg tgagtaggtt
     5581 gctgtaatgt agctgttagc atattgtcac tccttcctaa aagttaatta gcttgtttac
     5641 aattacatta tacacactag tgtgtataat gtaaagagag gttttttgct gatggatcag
     5701 ctgtgctaaa caaaagaaaa cgatagagta acaaaaaaag tcagcaaagt taaaaaggag
     5761 gaaacggcta gtccggttgc tttgcttgtg taagtttcgg ctttcttata gatcaaatag
     5821 gaaattatca gtgatattcc agtaaagaat aatgtactag ctgtcactaa aagaaatagg
     5881 ttaatcatac gatacactcc ttctgtaata ttggtatcag atacgtttaa atgcgtttct
     5941 gagctgtttt ggatcaatgg atgttttagc cctaccctaa aatcaaagcc cagaaaagca
     6001 tttaaaaacg ttcgtaccta atacgtttat attcatatac atatacataa acataaacat
     6061 aaacgtatac acgtttatgt ttatgtattt aagttttagg ctacttcctt aacatctcct
     6121 gctgatagtc gagtgaaccg agtgagtgat ccatcttgta gtctaaaaat aaagctacgt
     6181 ttgagtttat actgataaac agcgacatac gatccatccc agattgagta agttttacct
     6241 gcaactaata agtcatcaag gtttggttta ttgttttctt ctttaattct atctagttgt
     6301 tgcttaacca ttttttcttg atcaccgaca aagaatttca accatttttt cggaattttc
     6361 ttaaagtaag caggactgtc atttaagaag tagattggtt tgtacatttc atctcgaaag
     6421 atgaagctgt caatatcgta ggttccttct gcatctttga aaaggataat accataggtc
     6481 ttcgttactt tggtatctac gatttccaaa ccatttttgg cgtacccttt cttttcttcg
     6541 atcacttcat ttgctgatga ataagcacca taccatccca taaaaaatcg ctccttttga
     6601 aagttaatta gcttgtttac acttatatta tacacaccag tgtgtataat aacaagaggg
     6661 tattgttctt ttttaggtaa agtgggatcg taaacagtga ttttgttggg ttaacaaaaa
     6721 aacagacaag ccttatcagc ttgtctgtta cttataaact tcacttctgt gaccaataga
     6781 caatataaga attgtcactt catgctcgct aatatcagct attatacggt aggcacctac
     6841 cctatatcgc cagtagcctg atttattagc tgttagacct ttaccatgtt ttcttggatc
     6901 atcagtgcct acaagatttt tctcgatcca gtttaggata acctttgcct gaaacttatc
     6961 catttttttt agcgcttttt gagcttcacg ttcatatctt acttggtaag tcatcttata
     7021 agcctaactc ttttagcata tcttcgtggg aaatactttg atcgttttcc tcatgctctt
     7081 tcattagttc cttgtaggtg gcaaagtcta ctaacgtttc tgctgtttgc agggaattac
     7141 tacgcataaa ttcagaaatg ttatctccgt tgaattcagc aactttttca agaaaatctt
     7201 tttcttcttt agatacacga atacttatta ctggcatagt ttatcaactc cttttcaatt
     7261 acattgtact acgattgtat
//
LOCUS       DAYYKC010000122         7251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000122 DAYYKC010000000
VERSION     DAYYKC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.124"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHBBW_002675"
     CDS             99..746
                     /locus_tag="ACHBBW_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9154302.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHBBW_002676"
     CDS             1139..3784
                     /locus_tag="ACHBBW_002676"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9154303.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHBBW_002677"
     CDS             4094..5407
                     /locus_tag="ACHBBW_002677"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9154304.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHBBW_002678"
     CDS             5397..6050
                     /locus_tag="ACHBBW_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9154305.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHBBW_002679"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHBBW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9154306.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHBBW_002680"
     CDS             6947..7147
                     /locus_tag="ACHBBW_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9154307.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2395 a         1229 c         1593 g         2034 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt a
//
LOCUS       DAYYKC010000123         7247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000123 DAYYKC010000000
VERSION     DAYYKC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.125"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            961..1197
                     /locus_tag="ACHBBW_002681"
     CDS             961..1197
                     /locus_tag="ACHBBW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154308.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            1310..1552
                     /locus_tag="ACHBBW_002682"
                     /pseudo
     CDS             1310..1552
                     /locus_tag="ACHBBW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1642..1881
                     /locus_tag="ACHBBW_002683"
     CDS             1642..1881
                     /locus_tag="ACHBBW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154309.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            2028..6311
                     /locus_tag="ACHBBW_002684"
     CDS             2028..6311
                     /locus_tag="ACHBBW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM9154310.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            6410..6775
                     /locus_tag="ACHBBW_002685"
     CDS             6410..6775
                     /locus_tag="ACHBBW_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154311.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            6777..7229
                     /locus_tag="ACHBBW_002686"
     CDS             6777..7229
                     /locus_tag="ACHBBW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154312.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
BASE COUNT         2584 a         1121 c         1579 g         1963 t
ORIGIN      
        1 aaaaatttat aaaaggaggt ttcttgatat gataagaaac aaaaagggtt tgttgaaagt
       61 tgctttactg gcagcttttt tctaagtaac tttttgacaa gctcaatggc aacacaagcg
      121 gaagaagtaa tacaagaact aactgttatg aataacagct agataaaatg ggctatgtat
      181 gttatccaac aatttagtaa tcactagaag cctagcgaaa gttcgcttga taagttatag
      241 atgagtattg atttgtagtg tttccaaatc aaaagggtac agagccacat gaaatatgtt
      301 gtcctctgca gcgtcaggat gggaatccat accatggaga ttcggagatc tgtgagggaa
      361 tacttacgtg gttaatatgt cagttgtgtt ggacgtagta atggtcatag tgggtagatt
      421 gactgaattc ttatatgaga aaatgaaacc aacaagaaga tagggttatt tatacagaat
      481 tggggatctt ttctggaaaa gtaatcggat aatgcgagat tcgtacggtc ttttttaaat
      541 ttctctaaaa aaaacggcat cgaatgggtt gggtgattga taagcaatga atcatagact
      601 gatatagaat aaaaaatgat tctatttatc tttttacatc tatcgttttt tcaagtagcc
      661 gcaaattata tcagcattca acaatttttg tatacaagtt ctagtaaaag gatctgaaga
      721 aaaagttggt tgttgtgaag ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa
      781 tttgtgttat taggttagga caaagataag aagaagtatg aagatttaaa aaaggaatga
      841 ggaaaagata gtaatttccg attattggag tttttttatt tataaagaag aagcgaataa
      901 gttgtgattc ctagatttcg cgcgtgatta attaatgagc aaaaaatgcg gaaagtttta
      961 gtgtttaata cacgaggaca aagtttctta ggtattgaag gcacacgtgt aacggttgat
     1021 gggtggtgtg attgtttcga ggttggaaaa catgttgaac ttatggcatt tactgagcta
     1081 aaagataaga acaacgtgga atttttcgag agtgatattg gttgggacga tcatgaagaa
     1141 gtttatggaa aatttatttt gcggaaggga aattcttata cgaatgggga agcataagta
     1201 gcgatttgtt agagatcatt gataggattg aaattgtcgg gaatatttat gagaatctag
     1261 agttatttga ggtgatagaa tgagtgatgg ttttgaacaa gtatcaataa ttaaaaatca
     1321 agttcgggag attcttagaa aaaaaggcta tttggtagat agttattttg aaggagatta
     1381 cgaaacatgg gtaggtgtct atgctcgtcc agagaataaa cctacctatt tagatcctac
     1441 gacgtcagaa gatgcctatt tacaaaatag gtatcgagta gatggtttta agcaagactt
     1501 tgcggagtgg tttgaatggg aaattgaaaa cggagaagta aaagaagaat aagagtactt
     1561 gttgtatcaa aataagccag tagaacaacc aaaccagtag ctagtaatga caataatact
     1621 gaggaatcgg atcattatgg tatgattgat atttctcttc cagaagatct taagccagat
     1681 cctgtaacaa ttggcaatag tgtcatgatt tttgattcat tagatgaagc tatgtcatat
     1741 ggttggaaag aaattaagga tgaaggtagt aattggtatg gcatgagatt tggtgatggt
     1801 gaagttgcca aaaaagctgg tattccaatt gggaaatgga cagttgattt ccacgaagca
     1861 ggttcttttt ttgttctgta attatagtgg gagggtaaaa tcattgatga aatgtgttca
     1921 gataagagcg ccagataaaa atagaagaga cagttcacgt cgttatcact tttggtaacg
     1981 gcgttttgtt ttattatata aaattctcag gaaaaggagg aattcttgtg tatgcaaaaa
     2041 cgaaaatgaa ttggttgaaa gcactcgcat tggtggcttt ttctcaattc tgtttcttgg
     2101 tgggaactgc tcaagcacat gcagaagaaa cgacggctat acaacctacg atggaagcca
     2161 ttgcccaagt cgcttctgaa gtgacagaag tccaaccagc agaagccggg gtcccagctg
     2221 gtgaagccac tgttgaacag gcccctgtga cagaagcgcc aacggtcaat tccaaaacga
     2281 ctctcgatca gacgattggg gaaacacaac acccagggaa tagtcctggt acttcacaac
     2341 cagttgaaga aactgcctca attacaaccc ctgcttcaga aacgtccact ctagaaggag
     2401 aaaaagctgc cgaagcaacg ccttctgtgc agccagagtc ttctgagaaa caagcggaag
     2461 aaaagaaacg aatagaagaa acagtggaac aaccagcggc tactgctgac aaagaaggaa
     2521 aaaatgagga aaacgttgaa aaaacaatct cttcaaccgc agctagtcaa aatcaaccaa
     2581 cgaaaccaaa caaagaatct acggctagta ttgaaaatca aaagaaaaaa gagcagaaca
     2641 ttccaaaaac aggtacacct acactgttag taacagtacc aaccaatcgt tcggttcgtg
     2701 ttcgccgtgc aaccaatgca gctgttttag gtggtcggaa aattgattgg ggcgaaaata
     2761 ttggtgttac ttctagtccg aacaatctaa acttcacttt aaattatatg gttcgttttg
     2821 agattaatgg acaagtggcg ttttgtattg agcctggtgt ctcagccgca gaaggtcaag
     2881 tttatacagc tactgctttg aaaaactatt tgaaaaattc aaatgtacgg aaaaaaacaa
     2941 gcttgatctc ttatttagga tatgtcacaa ataaagacaa atccgatgaa caatacattg
     3001 cggcacaatt tatgatttgg gaagctttgg gcacaaaaat cccaatcaaa aatatccgtg
     3061 ttgattatga tcgtcgaaaa gcagaaattc aacgtttgat taataacttt ggtgaaaaag
     3121 caacttttaa taatcaaaca catacggtaa aaattggcga aacgttgaaa gtaaagaata
     3181 aaaatgattt tttgaaatat gtgaaagaca ttcaaaaacc aaaaggtgtc aatgtatcta
     3241 ttcacggcaa tgaagtatgg attacagtca caaaagaggc accggataaa gcaaaaattc
     3301 aattgaataa aattaaagtt caaggtgcgc cattagccta tcgtcgtcca ggagctcaaa
     3361 caattggtgt gttacatcca acagaaccta atgcgtctgt tattaattta caaattctaa
     3421 aacaaggaaa cattcgtgtg tttaaagaag atgcggaaac tggtgtgaaa gcacaaggtg
     3481 cagcttcttt agaaaatgcg gtctatggtt tgtatcaagc agatgggaaa aagattaaag
     3541 aaattacctt gaaaaaaatt aacggcaaag tacaggcaga aattaaagat cttgatcctg
     3601 gcaaatatgt tttaaaagaa atcaaagcgc caaaaggtta tgtgctttct gatgaagcaa
     3661 ttgaaattgt tgttgaacct ggaaaaactg tcaatgcgac cgcaaaagat caagtgatca
     3721 aaggaaccat tgatttagtg aaagttgcta ataaagattt agtcgattca acgaatccag
     3781 ataacaaacc aaaattagct ggcattgaaa tcactttgac gtcaaaaaca actggcaaag
     3841 tggtgaaaac cgttgtgaca gacaatgatg gttatgctac ttttggtaaa aataccgttg
     3901 tctttgatac gtacattctt tctgaaacca aaggaaaaga agggtataaa ctatttgaac
     3961 cacttgaagt cacaatttct gaaaatgggc aaactttcca ttatgtttta gaagataaag
     4021 taattgaaca acgtcttaaa gtggtgaaag tggatcaaga aacaggaaaa gtgattccac
     4081 ttgctaatac agaattcaag atttttgata ctttagccaa tcgttatgtc acaatggaga
     4141 ttccaaatga tacagaaaca acggatatct ttaaaaccaa tgaaaaaggc tttttcctaa
     4201 ctaacggtac acttacttat ggaaaagatc gctatcgttt ggaagaaatc aaagcccccg
     4261 taaattacgt tttgaatcaa aacccattag tgttctcgat taccaatgat acagaaccga
     4321 tcaaagtgat ccattttgcg aaccgtcaag cacggaaaag tgtaaaatta ttcaagtatg
     4381 aagaatggaa caagaagaaa acgccgctcg caggtgtgaa ctttaccttg tataacggta
     4441 aaaatcaagc aattggtgaa tataccactg atcaaaacgg ggaaatttta gtgaaaggtc
     4501 tagttgccga tgactattac ttcttggaaa atgccccatt agaaagtttc caaccaaata
     4561 cagataaaca agcattcacg gttgcattca aacaaaatgc gccagatgat ggggaaacgt
     4621 tagtggtttc tgtacgtaat tacttgattc caccaaaact agaaacacaa gcgtcaaata
     4681 aagccgatgg tgaaaaagtg gttgatccat tagaaacgat cgtcattaaa gacagtgttt
     4741 cttataccaa cttgtttgtc ggaaaagaat acaccatttc aggagtattg atgaacaagg
     4801 caacgaatgc gccaatttta caagatggca aagaaatcac aagtaccttg gcctttattg
     4861 cagacaaagt caatggcaca aaagaccttg aatttgtggt taatgctagt gttctgaaag
     4921 gtcaaacgat cgttgttttt gaaaaacttt tccgtgatgg cattgaagtt gcagcacatg
     4981 cggatatcaa cgatgaagcc caaagtgtga aagtgaataa tccaaccgtt cataccacgg
     5041 ctgtctttga agacggattg acagtcgcag acccaacagg attgtttacc ttaaacgata
     5101 ccgttgaaat taccgagatt cttccaggaa aagcatactt ggtcaaaggg atcttaatgg
     5161 ataaagcaac cggtgaaccc ttgcttgtgc atggaaatac ggtagaaacg agtgttggct
     5221 ttattgccaa agcagaaaat gaaacggtga tcgtaccatt tacctttgat ttgacaggta
     5281 taaatggtcg tgaaatcgtc gtgtttgaaa gtctttttag aaatgatgaa gaactagcaa
     5341 accataaaga catcaacgac aaagggcaaa cggtacgagt aactaaccca agtgtacgta
     5401 caacggctac acataaaccc actggcttga aagaggttaa tccattaacg aaagtcacca
     5461 ttacagacgc tgtgaaatac cacgatttgg tcgttggaaa aacatacaca gtcaacggtg
     5521 ttctaatgga caaagtaacg gaaaaaccag tgctagtaga tggaaaagaa gtcacaagta
     5581 ccttaacgtt tgtggcagaa acaacggatg gtacagtcaa tctagacttt attctagacg
     5641 cacgtgcgtt gcgaggcaaa gaagtcgtgg tctttgaaga tctgtttaga gaaggcgtat
     5701 tattggcttc tcacgcagat attaccgaca atgaccaaac catcaaaatc agtaatccaa
     5761 ccattacgac gaaagcgacg aataaaaatg gcggaaaaga gatcttggcc ttgccaaatc
     5821 aaactgtgct tgaatgggtc aaagtagacg gtcttgcgat tggtcaaatc tatcgcttaa
     5881 ttgttcaagg ctacttaaca ccagacggca caccatttga aggcactcat gcggaaaaag
     5941 tctttgcggc cgataaagaa atgatggaat tcttatttga attcttagtg gacggtcgca
     6001 atcttgcagg aaaaggcttg agttttgcgg aatggctcca agcgaaaacg gaagatgaga
     6061 aagaaacgtt tgaagaagtg gcgaaacaca atgtagatct aacaaacaaa gatcaaacgg
     6121 ttcaaattgt ggaagcgcca aaaccaccgc aaccagtgaa accagaacaa cgggttaaac
     6181 cagctactgt tgtaccaatg ttgccaaaaa caggtgaaca aacaagtagt atacttatgg
     6241 ttcttggatt agtagtatta gtcattgctt taggtataac actttataaa gtacgtaaag
     6301 ccaaagatta gtcatgagga acaagcggtt aggattttta accgcttgtt tgattgtaat
     6361 gtagaaatat ttagaattta ttttggtttt taagaggagg aaaaacttta tggcagaaaa
     6421 agcattgcgt atgaagaaag agtttttagg tcaagtattt ttattgggtt cacgggaacc
     6481 taatccacca aaggaaatta atggtgtgtc tgggcgatat gttcaattac aagcagagtg
     6541 ttcgcgagaa gaacagcctg ctttttttgt ttatctcaca gctgaagagg ataaaagatt
     6601 acgattacca gaaaaagagg tgcgtatttc atttgaagaa gcttattttg tttttcgtaa
     6661 agaacgagtg aatagtcgtg aaacaaatga tatgggacaa acagagcgtc gaacaactgg
     6721 ctatcaagat gttggtgtct ttttaactgg tgtaaaaatt ggggaggttt tgtaagatgg
     6781 gattaaaatt tccaaataat tatgacatta cagaagcatt agatgaaact tgtttaggag
     6841 ccgtacgctt tggcgcatta gaagagtctg atcgtgtatt tgataatgaa acaggcgatt
     6901 atacgggaga aattcgatca cagcgagcac aattagtttt tggaaatcca gatgaaccaa
     6961 agattgcacg acgagttaaa tttccagcag atgtagattt gactggtttt aaacgacgtg
     7021 atcctgtgaa attaattgat ccagaatatc atgtggtata tattccagca caagatggca
     7081 atcgtgaata ttcagattta gtgatttatg cgaaaggatt agagaaagta gaagctccaa
     7141 caaataatcc taatatgaaa caatcacaag aaaaaggttt agatcaaaaa caaccaagtg
     7201 ttgattctaa ggaaaaagag aaaaaataag gtttttttga aagaata
//
LOCUS       DAYYKC010000124         7141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000124 DAYYKC010000000
VERSION     DAYYKC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.126"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHBBW_002687"
     CDS             <1..615
                     /locus_tag="ACHBBW_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9154313.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHBBW_002688"
     CDS             612..1385
                     /locus_tag="ACHBBW_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9154314.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHBBW_002689"
     CDS             1382..1780
                     /locus_tag="ACHBBW_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9154315.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHBBW_002690"
     CDS             1965..2636
                     /locus_tag="ACHBBW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154316.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHBBW_002691"
     CDS             2695..3135
                     /locus_tag="ACHBBW_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154317.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHBBW_002692"
     CDS             3147..3815
                     /locus_tag="ACHBBW_002692"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9154318.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHBBW_002693"
     CDS             3879..4502
                     /locus_tag="ACHBBW_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9154319.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHBBW_002694"
     CDS             4558..4944
                     /locus_tag="ACHBBW_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9154320.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHBBW_002695"
     CDS             5157..6548
                     /locus_tag="ACHBBW_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9154321.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DAYYKC010000125         7045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000125 DAYYKC010000000
VERSION     DAYYKC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.127"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1194)
                     /locus_tag="ACHBBW_002696"
     CDS             complement(<1..1194)
                     /locus_tag="ACHBBW_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9154322.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNN"
     gene            1513..2238
                     /locus_tag="ACHBBW_002697"
     CDS             1513..2238
                     /locus_tag="ACHBBW_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154323.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            2228..2737
                     /locus_tag="ACHBBW_002698"
     CDS             2228..2737
                     /locus_tag="ACHBBW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154324.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2807..4255)
                     /locus_tag="ACHBBW_002699"
     CDS             complement(2807..4255)
                     /locus_tag="ACHBBW_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9154325.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4255..4971)
                     /locus_tag="ACHBBW_002700"
     CDS             complement(4255..4971)
                     /locus_tag="ACHBBW_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9154326.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(4952..5302)
                     /locus_tag="ACHBBW_002701"
     CDS             complement(4952..5302)
                     /locus_tag="ACHBBW_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9154327.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            5446..6219
                     /locus_tag="ACHBBW_002702"
     CDS             5446..6219
                     /locus_tag="ACHBBW_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9154328.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            6390..6756
                     /gene="ssrA"
                     /locus_tag="ACHBBW_002703"
     tmRNA           6390..6756
                     /gene="ssrA"
                     /locus_tag="ACHBBW_002703"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2059 a         1379 c         1252 g         2355 t
ORIGIN      
        1 gttgtttaag cattccaatg gtccgtttga atagctgtag tgaagcgcat tttggatctg
       61 tggcagaaac tttttaaacg ttcgtaaagt ggttgtatag gtttctggca gaccgctaat
      121 gtctctagtt aaacaatcct caaatagtga ctgatctctt ttctttaccg cataaagaat
      181 gtcttggtat aactgatagc cttggcgtaa gttgggacaa gcagctaaca gtcgatcggc
      241 aatctcactt tcagtcaaat aatctttaaa actggcacgc caaacccgtt tttcatagtt
      301 taactttgct tcttcttttt gtaggagttt ccaatagcgc ttcaattttt tccctaaccc
      361 tcgttgagag tgggaatttc ctttaagtcg taccgtggtt tctttcactc gatgattcaa
      421 gaaagttctg ccaatatgtt ggacgatgtg gaaacgatcg atgatcaact gagcattcgg
      481 gaaaagcttt ttcaccaacg aaacataagg cgcatacata tcaataacaa tcaattgaac
      541 ggcttcacga gtcgctaatg aaaaccgaga aaaatagcgt tctaaataag gcaagcgtcg
      601 attttcgaca atatccaata atgcatggct ttgtccgtcc atcatgataa aactcattgc
      661 actggcaaca cttcgaaccg atttgaattc atcaaagcat aagacctttg gcaaagtctg
      721 tttgaaagga ttcaatgggc ggtagaatcg cttcatgaca cgatagaccg aagaagtgga
      781 aatatttttc tgacgagcga tttccgtcat ggaagtattc tcttttaagc gagcaatgac
      841 agaccaacga actttttgac taatgaaaca gtgtttttca gcaacagagg tatccgctac
      901 gaatgttctt tggcaagact tacatttaaa tcgttgtttc ttcagctcca gataagtgcg
      961 atactcagac acatcattca gtaacaaccg aacggttgtc gtcccccaac gaatgatcct
     1021 agaggggacg acacaaagac aatgggaaca ctcttctggc ttgtacagaa gagttccttt
     1081 gtaaatcaag caaatttgcc ctttgtattt ttctttagtt aaacaatttt cctcgaaata
     1141 gatatttagg tctagaatat ttagggtatc tttgataagc tgagtatgag acatcttgaa
     1201 tcctcctatg aattggtagc caaattaatt ctaaaggatt tcagggtgtc ttttctatat
     1261 ttttttatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagcccaaa
     1321 attattgtcg ctgctcctct tttcgaataa acagtgttca aaagcttgca tctacggtaa
     1381 tagaataggg cagcttcttc acaacctctt gctttttaca aaatattttc tggttttaat
     1441 tcgtgatttc actaaaggaa tttcatactt tattcatatt cattttctat actttttata
     1501 gaggtgaaaa gtatgaaaat aatccgctat gctactgctc gattaattta ttacaaaaag
     1561 cagacaatac tctattgttt attttcaagc ttttctgctt ttttattgat agcatgcctg
     1621 actctttacc attcgcaatc ggctttagcc aatcaagtcc aagatcggct ttcatttatt
     1681 ggcgcttctg catctacaga gcttcttccg gctttagaat tagctagccg attttatata
     1741 aaaggcatat tcagtttatt ttttctattt gccttccttt ttatcttttt cttttactat
     1801 tcagtcaggc aaaacaagca ggaattgatc aattggcgcc tgactggttt ttctaaaacc
     1861 aaattattcc tttttatttt ctggcagctg ttctttcctc tctttctttg ttgtatcttg
     1921 gttttattgt ggatcgtcag cttccagtca gtttatgaag cgcttcttca aaggatcaat
     1981 tttcttgtct taaagactac tgaactgcca gatatccgtg atatcttgac ttcggtaagc
     2041 ggacttacaa ttccgatgga tcaacagact ttttttacca tcgatttcca aaatgatctt
     2101 tttttcacag atatacttaa gggatttgcc caaactctta ttgggctcag tggatgctgt
     2161 ctcattctta gtctattccc tttcactttt tttatccatc atttgcaaag taggaggcct
     2221 ggacgtaatg aaacctgaat atttgattca tgctgaacac ttgaccgtta ttttgtgtcc
     2281 tctcgaaaaa cagcataaaa tattggagca cttgaaaaaa gaggttcttg ttgcaaaact
     2341 ggataatttt gcgatcatcc aaaaaaattg gccgatgttt ccttatctga atcttaaaga
     2401 ccatgtcctg ttggatgtac cggaaaaaga aatcaaacaa gatcgtttgg cctatcaaga
     2461 aaaacttgat atctctcctt ccttgcttaa ttgtgcggca gctgagttga cgtcttttga
     2521 aaaagtaaag ctgcaattac ttcatgcctt gctttccaag cgaaacaata ttgttattga
     2581 agatacattt gatgaactta gtgtattgga gatccaagag ctactccact tgctttctta
     2641 tttagctcat gaagaaaatc aaggtatctt actctttact catgatgcta ctattgctca
     2701 aagtccttat attgatcgtt tagagcccgc gggctaaatc aatcatttta tagtaaaaac
     2761 aagcggtata gaagatcgat tcatgatctt ttataccgct tgttttttac tcttttttga
     2821 tttctacgtc aggcaaacga acagcgaatt ttgttccttt tcctagttcg ctttctactt
     2881 ctaattttcc tttatgcaag cgtacaagtt gctgcacgat tggtaaacct aatcctgatt
     2941 caccatattt tgtatttttt cttgaaggat ctactttata ataacgatcc cagatacttt
     3001 tcatttgttg ttcagacatt ccaattcccg tatcttcaat cgtgatgatc gtttcaagat
     3061 agcctttttc taaagtaatc gtgatttggc cgttttcagt aaactgtatc gcgttttgaa
     3121 taatattgac catgatttgg acaaatcggt cataatcagc gtaaacatca atatcatcaa
     3181 cggctttcaa tgtcaatgta tctcctgctg cttctgcttt agcttctaac tgtgcaacaa
     3241 tatttttcaa tgtttctgtt ccattgaatt ttttaatgac catactgatt tgattcgtac
     3301 ggattttttc ataatccaag ttttcattca ccaaacggat cagacgctct gtttcatttt
     3361 tcatcaattt aatcgcgttt tctcgctgat tttcagggat tgcattgtat tctaaccctt
     3421 ccagaagtcc gttgattgtc gtaagaggtg ttctcatctc gtgagaagca tctgccataa
     3481 attgtcttcg acgattttct tgttcttcaa tttctgcttg agattctttc aatgaattcg
     3541 tcattttatt gaaatcatct gctaactcat caaattcatc tttgtcatgt acggggagct
     3601 gtacatcaaa attaccattc gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct
     3661 taacttggaa tgctgcaaaa gcatagctgg caataactgc aacgatactg gaaaaaataa
     3721 atcctttgaa caggttcagt gtaattggac gaacgctgtt atcgatattt cgcgcaggtt
     3781 gactaactac aagtgctcca tagaattcat gattcaaatt aaatggcaca agtgcgtaag
     3841 aggtcgcctg tgcttgtcct aaaatatttt tattcgatgt gaatttttct tgacgtccat
     3901 tcctcagatt tttccattga tcagctgtta aggtaaaatt cagtaaagct gtattagtcg
     3961 gataaatgac acgttcgttt ttatcgataa atataaagtt cacattttgt tctgtcagta
     4021 cttgctctgt cagcagcaat gcattttgaa acgcctgatc ttgactaaac tgcgggagcg
     4081 tatcggcata ggtttgcgtt gtcttttcaa ctgattcagc atatccaaac agttgctggt
     4141 aattgttttc ttcaatcgtt tgttttgtca gttgtgtaaa agaaactcca acgatcaata
     4201 agatcaacgc gaccattccg caaaaagcta agagttgctg atacaaataa cgcattaggc
     4261 aacaccagaa tcatcgaatt tgtagccgac tccccaaaca gtttggatga cttgtggtcc
     4321 gaccttttcg attttttgtc gtaatttctt gatatgggcg tccactgtcc gttcatcacc
     4381 aaagtactga taatcccaga ctaattcaag caactgctcc cgagagaata cttgacgtgg
     4441 tttttttgct aaagtaaaca atagatcgaa ctctttaggc gtcagtcctt caattggccg
     4501 gttgtccaga tacgcttctc gtgttttcgt attcatcttg aaatgactag tctcgatatc
     4561 aaaatgatca tctgaaactg ctgcttcttc tcttgcttct gccaactcac tacggcgatg
     4621 caaagctttc attcgagcaa taagcgtcag aggactgaaa ggttttgtga cataatcatc
     4681 tgcccccatc tctaagccga tcacctgatc gctttcagaa tctctagctg tcagcatgat
     4741 aatcggcaca gtacttgaaa ctttgcggat ctctcttcct actagcatac cgtccagtgt
     4801 gggcaaattc aggtctagtg taatcatatc ccaatgttgt gggttttcta aaaaggcatc
     4861 gagcccttct tttccatcat acttgaaagt tgcttcccaa ccttcattta agaaaaacat
     4921 ctgcatcatt tcagaaacag attgattatc ttcaatcatt aaaatattca ttatcgttcc
     4981 ctccagtttc caggtcatat ttctgtaatt tcttttctgt cataataaaa ataggcataa
     5041 ttggaaatac tagcagaagc atttcaatca gaaaataatt cgtatgccca gatgtcttta
     5101 aataatagcc gatcaatgcc atcagtaagc ccataagaaa aaaccagttt ccgcttgtct
     5161 tttgagccag ccgccaatga gcgtctgttt ttgcggcaag aggcgaatga tacccataaa
     5221 aaggcagatt tccttttgag ggcagaatcc gaaaaagact gccaattatt aagaataaga
     5281 taccaatact gaaaaaaatc attctttccc tcctaaattc tcttcttata gtatactcta
     5341 aattgttgac tgaaccaata tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat
     5401 tctttgttaa gataaactta ttccaataaa aaaggagttt tatttgtgtc aaattatcgt
     5461 gcaatcactt tctttgatct tgatggaaca ttactagatg cgaaatcaaa aatcacaccc
     5521 gaagtggcaa aagctatgaa cgcattaaaa gaaaacaatg ttctccccgt catcgctaca
     5581 ggacgtacag aagcagaaat ccactctatc atggaagatg cgggcattac ttctgcggtc
     5641 accatgaatg gttctttcat tcgagtagac ggcgaagaaa tctattctca aacattttct
     5701 tcagaagaat gtcaaaaaat gtatgatcat gtacagttga acggtcatga actttcttac
     5761 tataacgatc agaaaatctg gtgtaccggt catagccaaa caatgatcaa tgcttacgac
     5821 ttcattcatt cagatgttcc ggaaattgat cctttaggct ttaaagaaaa cacagtaaat
     5881 atgttgctga tcttatctca atcaggtgac gaatactatc acgaacattt tcctgagcta
     5941 actttttatc ggaatggtcc ttactcgatc gatacagtga agaaaggaat ctcaaaaggg
     6001 gcaggtgtca aacgtctgca aaaagaactt cagctggaaa aagttcctac ttttggattt
     6061 ggcgatggtc cgaatgattt tgccttgttg gaagcttgcg acaacaaaat cgctatggac
     6121 aacgcctatg acggattaaa agaactttct acctttatca caaagaaaaa tacagaaggc
     6181 ggaattgtcc acgcattgaa gtattttgat ctgatttaag atcttttttt gaagctacct
     6241 cttttcttaa gaaaatcgct tattttttct taagaaaggg gcagttatta tataaataat
     6301 gggatatgat tcgctttttt attgaattta tcccaatata tgctatacta attttgtatg
     6361 aaaaatacac gtaacccttg ttagctaacg gggacgttac ggattcgaca ggcacagtcg
     6421 agcttgaatt gcgtttcgta ggttacgtct acgtaaaaac gttacagtta aatataactg
     6481 ctaaaaacga aaacaactct tacgctttag ctgcctaaaa acagttagcg tagatcctct
     6541 cggcatcgcc catgtgctcg agtaagggtc ctaactttag tgggatacgt ttcaactttc
     6601 cgtctgtaag ttgaaaaaga gaacatcaga ctagcgatac agaatgcctg tcactcggca
     6661 agctgtaaag tgaatcctta aatgagttga ctatgaacgt agatttttaa gtggcgatgt
     6721 gtttggacgc gggttcgact cccgccgtct ccatatatgt atggttgaga cagataaaca
     6781 tgtatgaaaa acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga
     6841 gaaatacaaa attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat
     6901 ccccaatacg tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac
     6961 actctttttg aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca
     7021 tgttccttca aataagtttt tttaa
//
LOCUS       DAYYKC010000126         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000126 DAYYKC010000000
VERSION     DAYYKC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.128"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHBBW_002704"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHBBW_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHBBW_002705"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHBBW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9154329.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHBBW_002706"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHBBW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHBBW_002707"
     CDS             complement(2143..2373)
                     /locus_tag="ACHBBW_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9154330.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHBBW_002708"
     CDS             complement(2603..3421)
                     /locus_tag="ACHBBW_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9154331.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHBBW_002709"
     CDS             3582..4271
                     /locus_tag="ACHBBW_002709"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9154332.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHBBW_002710"
     CDS             4285..5787
                     /locus_tag="ACHBBW_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9154333.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHBBW_002711"
     CDS             5800..6276
                     /locus_tag="ACHBBW_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9154334.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHBBW_002712"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHBBW_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYKC010000127         6607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000127 DAYYKC010000000
VERSION     DAYYKC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.129"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHBBW_002713"
     CDS             complement(105..629)
                     /locus_tag="ACHBBW_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9154335.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBBW_002714"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBBW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9154336.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBBW_002715"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBBW_002715"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9154337.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHBBW_002716"
     CDS             complement(2351..3031)
                     /locus_tag="ACHBBW_002716"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9154338.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHBBW_002717"
     CDS             3157..4026
                     /locus_tag="ACHBBW_002717"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9154339.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHBBW_002718"
     CDS             4052..4567
                     /locus_tag="ACHBBW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9154340.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHBBW_002719"
     CDS             complement(4614..5177)
                     /locus_tag="ACHBBW_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9154341.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHBBW_002720"
     CDS             complement(5232..5411)
                     /locus_tag="ACHBBW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154342.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHBBW_002721"
     CDS             complement(5488..5997)
                     /locus_tag="ACHBBW_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154343.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHBBW_002722"
     CDS             complement(6093..6473)
                     /locus_tag="ACHBBW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9154344.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1857 a         1393 c         1170 g         2187 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcc
//
LOCUS       DAYYKC010000128         6590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000128 DAYYKC010000000
VERSION     DAYYKC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.130"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHBBW_002723"
     CDS             complement(1273..1938)
                     /locus_tag="ACHBBW_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154345.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ACHBBW_002724"
     CDS             2118..4100
                     /locus_tag="ACHBBW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9154346.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHBBW_002725"
     CDS             4104..4742
                     /locus_tag="ACHBBW_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154347.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHBBW_002726"
     CDS             4770..5198
                     /locus_tag="ACHBBW_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154348.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHBBW_002727"
     CDS             5655..6566
                     /locus_tag="ACHBBW_002727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9154349.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2265 a          999 c         1219 g         2107 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac
//
LOCUS       DAYYKC010000129         6577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000129 DAYYKC010000000
VERSION     DAYYKC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.131"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..279)
                     /locus_tag="ACHBBW_002728"
     CDS             complement(52..279)
                     /locus_tag="ACHBBW_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154350.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(446..2416)
                     /locus_tag="ACHBBW_002729"
     CDS             complement(446..2416)
                     /locus_tag="ACHBBW_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154351.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2431..3297)
                     /locus_tag="ACHBBW_002730"
     CDS             complement(2431..3297)
                     /locus_tag="ACHBBW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154352.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3287..3520)
                     /locus_tag="ACHBBW_002731"
     CDS             complement(3287..3520)
                     /locus_tag="ACHBBW_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9154353.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3532..5688)
                     /locus_tag="ACHBBW_002732"
     CDS             complement(3532..5688)
                     /locus_tag="ACHBBW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154354.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDAPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5708..5929)
                     /locus_tag="ACHBBW_002733"
     CDS             complement(5708..5929)
                     /locus_tag="ACHBBW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154355.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6039..6377)
                     /locus_tag="ACHBBW_002734"
     CDS             complement(6039..6377)
                     /locus_tag="ACHBBW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154356.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         1866 a         1285 c          895 g         2531 t
ORIGIN      
        1 gactgagatc acctttcaat ctaaatacac ggttcttttc tggtaactta ttcatgcttc
       61 aaccacctca tactgttctc tcataccttt gacttcatta aaaaaaactc tcgctttttc
      121 ttcatcagta aagtttttat acaactgatt acaataatga ggtttttgaa gtttctgaat
      181 acctatacta acttcaaatc cttcttcatg ttgaattagg attacttctg tgtctctacc
      241 tagaaatatt cgatctaaaa tttttatgtt tggtttcatt atttaacctc cactataatt
      301 atttcactca ataaggtttc actttatgaa atttctatag ttctcttttc acaaaacgta
      361 ccatgttctc taagactgtt tttagtctca aatatacatg ttttttctta tcctttcctt
      421 atacactcaa aatcaaagat aaaacttaaa gaataaactc tccaataaca aaattattag
      481 tcagatccac tggtctcctg atctgaaatt tattacttct tcctgttgtc aaatactgtc
      541 tcaaatcaac agaaagtttt tgatcatctt ctccttttct agcaggagca actttttttt
      601 catacttcat taactctcca ctggtcatct ttccaatgat agtgtaatta aaatcacttt
      661 caaaactttg gtaaattttt ccatctgtat tgaatatatc agctgtttca atatcttctt
      721 tgtattcatt gtcttctcct gaaccgattg tatcaaatag aaaggcaaga cgaattccat
      781 cttcttcaag cattgttaat ctttttttaa ttagcccaac ggtttcaata gataacttat
      841 cgaagccgtg aatcataaca atatcatctt tttttgcttg tgatgtgatg aaatcaaagg
      901 tatttaaaaa ttgagcttcc ataacattgg tttctgctct tagatcacct aagttatagt
      961 aaaactgtaa tttgtcactt ttaccgattt ctttcagtgt cgttctttga ttaaacatcc
     1021 tagagaagct gtctaatgct gttctcaatg tgtcttttaa tacaataata cgatcgactt
     1081 ctttttcagt accagatttt ttggcttttg tttcagcatt agtcaaatag ctaataaaat
     1141 ccccaagaac tggcattgta tcactactgt tcaccaaaag ttttgaacgt tcttcataat
     1201 taatatccca aagtttctga cttatataaa agttacctaa ttcttttttc aataaaactc
     1261 tagttgcaga atctaatgtt ctgcctgcta acaaataaaa aatctgagtt agtttatcga
     1321 gattccgtct atataaatca ccaatgtcct tttgattacc aaaaagctcc aaagagttaa
     1381 gaccttcttc agccaaatta accttttcta ttacactgtt taacgaaggt ttggcaacaa
     1441 atgttctacc agttggatca ccaaagtaat caaatttatt taagacaata tgaaaagttc
     1501 gtttatgatt tgccattgca ttattagcaa tcaattgccc ccaaagtgat gatgctgaga
     1561 ttttttcatc atagccttta attgtagcgt ttttgtgtga tgccactaaa actttacttg
     1621 taaaagaatc atccagtgcc ataaaagcta caccacttgc gtagctagta gacatagtgc
     1681 cgatagccca tccattcgca tcatctaaac ctttgcgaat tgcatgatca tttcctgcaa
     1741 aatccgaact catagacgta tactcataga cattcccgat tggttcactt aaaagtctat
     1801 caaacagatc acgttgatct cctccatatg acataagctt taatccatac attttttcat
     1861 catacaaccg ttggagttta attagctgtt cgctgatttt attaggagaa tcggatacta
     1921 ataggatttc tagcttccaa tcaataattt tatttgccat gctgtctagt aaagatgctg
     1981 tattcaaatc cataatccgc ataacagttt tgctaatttc agcatctgaa ttttttcctt
     2041 ctaaattatt ttcataacta ttcgttgatc ccccaacatt ctcgtgcatt atttcttgtt
     2101 gatctttttg tgacactggc ttatctgctt caattagata ggcttttacc ccttctactc
     2161 tcacttcagg aatagcttta accatccaac caaatccata atttctattc attccataag
     2221 tattcactaa tttcacaatt gttccccacc gcttacctgt cctaatgtaa gatggtgtaa
     2281 attgataacc aactaaagat gttaaatcac taacaaaggt attattcact acctttgaat
     2341 ttctagtttt ttgttctttt ttagtttttc tctttttttt gaaatttaat agttgtgatc
     2401 tacccataat ttccatttaa tctctcacct ctatttcagt gcataaatat cagataatat
     2461 ttcttttgtt tgtttagctg acaactgagt gacactataa taaagtcctc tactcgtata
     2521 gttttctaaa cgttccacac cttcatccaa taccatctta gattgagatt tcaagataag
     2581 atattggatt actgttgttt tcacaccatc aatatattct gatacaaatc gttcttgctg
     2641 atctattatg tcaaagactg ctttgttact ccttgcattt cgtttttgta ttttcatatt
     2701 tcgagtttgt ctttccgtat tctgaggttg agatgaagtg attttgattt cagctaagga
     2761 acgatctctg gtattttgat attgtattga tttcgattcc tgagccataa cttcttctgg
     2821 aaaagcggtt agtgatgtag ccccatctag attgaacatt aatgctacac tcccatctcg
     2881 atatacgatt gttccatctt gtttcacttt taaaatctct gtgcctcgaa atggtttctt
     2941 tttccccata gatactcttg ctgaattaac tactgatttg aacagtctaa tagaaccaga
     3001 aatcaatgaa ccactagatg gatcaataaa catatactgg ctaattagaa taagaaatcc
     3061 aattatcatt aataacgttg gaacaaaaat aaatttcagt ttaatcgtat cagaaactaa
     3121 gtaaacaatt gatccaagaa caacgactgc aacaactata gctgatacaa ttaatctttt
     3181 tgctgtaaca gaataggtgc tattgacatg cttttcaagc cattcaactc tgacaattgt
     3241 ttcagaaggt ttttgatgta agttatgttt aaatccatag tttgagttat ctgccatcag
     3301 aaatacttcc tttcacgttc aacttacggt aaacacctac agttatcact attgcaacaa
     3361 taagcaagcc aattaaagat attgacttgc tcattgtctc gttcgtttgt ggtagttcat
     3421 ttacctgata cacgagctgt gcttcctgta agtgctgaga agaggtcatg ttttcttgat
     3481 tgttttgaat tactggataa actatttttt cttctcgcat aatagccctc cttagttatt
     3541 atcagataag tgagcttgaa ttttgtactg atttccgtct ggagttttca caactgcttt
     3601 ttctccatca atagaaatca cttcagcctt agttgttttc ccatcaatac tgatgacaac
     3661 atctttccca actttgaact ctgtagttgg tgttgcttca tagctactat ttgttgaagc
     3721 agagttcgta tttgtcccaa tcgaattttc cactctcgaa atagatgttt gagtttgagc
     3781 ctgtgcttgt tgattattat gactaataaa gtaggacgat cccaagatcg ttccgcaagc
     3841 cattgcagcg acagaaatga tagtagccat ctttttactt gtaaaatcac gttttgaact
     3901 tgattgattt tctgtagcaa ctgctaatga tttcgttttt tctgccattt gagctagatc
     3961 acttcgggaa cgagataagt cttctcttaa agcttcactt tgttctgcca acttttcgct
     4021 atttaattta taaccctctg ctaaatcttt gacctctttc attcgatctt tatattcttc
     4081 gatagttttt tgttgctcta caagttttgt ttctaattct ttcgtgttgt tagctacctg
     4141 cttttcaact tcttctttgt tcgcaaaata tttgacaacc acatctgttg ttttctcttt
     4201 tgtatcatgg aactcagctt cattttctga ctttgttttt tcgacaatct ctaaagtttt
     4261 ttcttgcacg tctgttaatg atttttcaat ttctggtttc aatgaattga taattcgttc
     4321 caccacaact tcttttgctt cttggctttg tttcaatgca tcgtttttaa tagattcaat
     4381 tgcttcttgg tactgttgat caagactttc ttcagttgct tttctatatt catcttcttg
     4441 ttgtttaact tctggtaaat gctcagcatc ataccgttgc tctagtttct ttagttcagc
     4501 atccaaataa gcctgtttcg ccatatcata atctttacga atgatattgg atttttccat
     4561 cactaaatca tcaattttag atgcttctgc ttcgtagctc tctttagcaa ccgatagtgt
     4621 atttgtaaac tgattttgac catttaatga ggttgcttcc tctatccgca aaagctcatc
     4681 agctaacatt tgctttcctt tgaggatcaa ttgctccttc gtcttattta attcactgtt
     4741 gattttatta ttagcaatag agatgcgagc gttcatactc tctttagcta aaaacagcaa
     4801 tggtttcaac tcaggtatgg atagcatctc ctcgtttaaa tcgacagggt cattaatctc
     4861 atctaaaact acaggttgaa atttgtcagc aatgatctgt tcaaaatttt tttctaaaaa
     4921 atcagcatct aagtttttcg gtacatcact ttcagatgtg attttttcag cttcataaac
     4981 ataattttct ttgtcacttt gggattcggg tttttcatca atttttggtt gttggtaggt
     5041 ttctgtttgt atttgtgttt cttctatgct tacttcttgg ttttctgctt cggttggttt
     5101 gttatcatcc acaaaaagag cttctttttc atctggtaca ttttctgcta cggcttcaaa
     5161 ttcaacttct tcagaagagt tgaaggtctc agcaattcgt tctaatccat tagtagctac
     5221 ttctaattgc tctactgcgc tatagaagtc tgtagcttct tcaacttctg attcaaacac
     5281 attaggagca tccggaataa ctaataattg accactacta tccataaaac ggacacctgc
     5341 taatagcttc tctttaaatt tttctggtag atcagctaca taattcaaac ttgacttcaa
     5401 tagaacgaca tacttctcag aatcgagaat agcatcatcc tcatctataa ctccctcact
     5461 gttgagcttt tgtacatctt caattaccat ataatttttc tttggtaaat ctttggtcag
     5521 caacgcatcg aattctttcc atacctttgc tttagctttt tttgccttcg cttctttttc
     5581 ttcttttttg tacacttcga gtaattgtgg cttcaaagag agtgcaacta caggaactgt
     5641 ttctgccaca taattaatag attcggtaat atctttgaat tttgccatta ttgtaaaact
     5701 ccttttttca gtcaatagtt cttttctgtt tatctagcat atatacatat gctttttcga
     5761 atttaataag cgtttcaagt ctggcttttt caatcttttt actcccattt cgataatcat
     5821 agaactggtt aacattcata ttcattatct ttgccagttc ggtacctttc agactgctca
     5881 ttaatacttc gttggcgtga gctaacattt tttctctatc aaaaaccatc ttacatttac
     5941 tttcttccct cacttctaaa ctacgcttgg agcgtagttt tttggtaaaa agtaaattga
     6001 acaaatcggc atattatcca aatttgttca attcactttt aacctaacag ttcatttttt
     6061 ggaaattttg aaactagata gataccaaca actggaataa gtgctgatat gatattagct
     6121 ttttggttca aatcattacc tgttttagta cccattgtgt aaagcataac ggttgtaatt
     6181 acaggaataa taaagattgc aataatcgcc ccaacatatt ttaatccatc tccccatttt
     6241 gcatcaccaa aaaatttcat tgcagtgaat cctggtttca acgaaaaaac gaatgccaag
     6301 aaatacaaaa caacttgtac taaagttttc atatcaggcg tcattccgct aaccaaattt
     6361 ttaatagaat cgaacatata aaaacctcct ttttaatatt tttaataagt taattatatc
     6421 atcgacaaat cttatttgtt cacttgtcaa tgatctaact aagaaaaaga cgtctcgata
     6481 tttacatctt tatgttatca taataacata aattttcgat gattaaaaga gattttccat
     6541 taaattattt cacttttttt attggctaaa aatgctg
//
LOCUS       DAYYKC010000130         6411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000130 DAYYKC010000000
VERSION     DAYYKC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.132"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2602
                     /locus_tag="ACHBBW_002735"
     CDS             <1..2602
                     /locus_tag="ACHBBW_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM9154357.1"
                     /translation="RTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYNPI
                     KQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEK
                     RIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYN
                     PSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSR
                     VKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSN
                     GSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFK
                     LNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQ
                     TNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDS
                     FGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVT
                     KANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGG
                     VPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLEN
                     YFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITKI
                     EATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINS
                     YEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKV
                     NIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILL
                     PSSLKSYFRL"
     gene            2784..3023
                     /locus_tag="ACHBBW_002736"
     CDS             2784..3023
                     /locus_tag="ACHBBW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154358.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3039..4412
                     /locus_tag="ACHBBW_002737"
     CDS             3039..4412
                     /locus_tag="ACHBBW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9154359.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4416..6050
                     /locus_tag="ACHBBW_002738"
     CDS             4416..6050
                     /locus_tag="ACHBBW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9154360.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6213..>6411
                     /locus_tag="ACHBBW_002739"
     CDS             6213..>6411
                     /locus_tag="ACHBBW_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154361.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2245 a         1049 c         1292 g         1825 t
ORIGIN      
        1 gagaactttt tctacattaa acaagaaaac cgtttctcat attttttcta ctgcagattc
       61 tcaagcaatt attaactatt tagaaaaaca gaaattgtct gaaggtacag taaacataac
      121 ttataatcct attaaacaag aggtgattac acaaaaaaag cattctgtga gagacaatga
      181 tattgaaaaa attcggtttg tatttattga tattgaccct aaaaggaaag agggaagtgc
      241 aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa caagtagaac gttatttgaa
      301 agaaaagaga atagaatcat ttgtaaaagt tgatagtggg aatggctacc atatttttct
      361 tcccataaat gaacaaccaa ataatcacga gacgattctt actattcaga attttcttca
      421 attacttcac cgaagatttg gagtagaaga tgaagttgat attgatactt ctgtttataa
      481 tccttcaaga ttatgcaaat tagttggtac accagcggtc aaaggaaagt ccacaaaaga
      541 gcgtccgcat cgtttatcta gtttcctttc tattcccgat aacatagata gaagtaattg
      601 ttttaaaatt cttgaacaga tagttgaaga aaattccatg atgtcaacct ctagagtaaa
      661 aagccatcca tcttttagag aagtgttacc ttttattcaa gcagactata aacaatggtt
      721 agctcactat cctgaattaa cttataccat aaaagagggc gatcataaag gagttacctt
      781 acttatattt gacgaatgtc cattaagaaa acacacgaat aattctaatg gttcttgttt
      841 gtctgttacg gctgataata agattcgatt tcattgtctg catgcttcgg ataaaaataa
      901 agatattcaa gactttgtaa aaaaatatcc gttgccaaaa cctgtaaatc cttctgtaga
      961 atcattagaa tccaatcaag aatattattt tgaccagttt aaactgaatc aagaaggagt
     1021 atttttggtg aaagaaggtc aagaagaaaa agttgcttgt ccgatgttta ttaagcaaat
     1081 acgcaaaaat atcgaacaaa aagtcattac acttgttctt gcctattatg tcgatcattg
     1141 ttgggatgaa atagaaatag aaggtggatg tttacaaact aataatttta aacaattagc
     1201 aaaacatggc ttaatcatta aagctagaag agaagctgaa gtcgttgact ttttacagat
     1261 tcaacgggag aatgctccaa tcttttttga acatgaatct cttggctgga aaaaagaaaa
     1321 tgaagcaatg atttttcagt tacaagattc ttttggacgt gaaattcctt ctgtattaac
     1381 taaaaactca tgctaccaat taacaactaa tggtacagaa gcggaatttg acgcattggt
     1441 tcataagtat gttttaggaa cgaacttaga acttgcttgg tcgattggta ttgcttctat
     1501 tgtgttaggc tatttaaacg ttacgaaggc taatactttt ttgagtttac taattaacct
     1561 acagggcaag agcacaacgg gtaaaaccac tacgttacac tttattgcct ctttatttgg
     1621 aaatccaact acggttctta gaaatatgaa tgctaccaat aaagccatta taaagctagc
     1681 atcaaacaat cagggaggtg ttccactaat actagatgag ttaggagcag caactcaaaa
     1741 taactttagc tcgttgatct atcaattagc ttcaggagaa gaaaggctaa gattagattc
     1801 taattgtcag cttatgccac aagagacttt ttctgttttg acccttacct cttctgaaga
     1861 gcgtttggaa aattatttca atgagaaatt aactggtttg aaagttcgat atatcgaatt
     1921 ttctgatatc aagtttacaa aggatgcgaa tcacgcagag aaagttaaaa gtctttcttc
     1981 taatactttt ggttggggta taaagttatt tcttttgaaa ctctttgaac aagggattac
     2041 aaaaattgaa gcaacttttg tcgaagctca aaaaaaacta tgggctgttt tacctgaaca
     2101 ttctttgaag caacgcattg gtaataatct agctttgatt tatagtggag ccctcctaat
     2161 tcaagatata ctgggctatc ctttacgatt agagcatata cagacggtgc ttattaattc
     2221 gtacgaacag ttaattgtag atactagttt aggaagtgag caaacggtat ttgaccgagt
     2281 gaaaacattg ttggtgatga atagccataa atttttaaca aacagtgaca acaaacatac
     2341 gcccagtacg atttggggaa aagtatcaat tcaaaaaaat ggggatatta aggtaaatat
     2401 ttttcctatc ccgtttgagg acttgttaag gcgagaattt caagtaaagg atctaaccta
     2461 tctttttagg caattgcttg attcgggtaa tatgtgtgca gaaaaaggga gaagaacgaa
     2521 acgtattcac atgaatcgtc aagttcttcc aacatacgag attctattac cttcttcact
     2581 caaatcgtat tttcgattgt aatttagaat aaaccaaagt atgaaagtga ggcaccatgt
     2641 tatggaaata tttcttattg atttagcaaa acaactgatt acttatctgt atgatagcga
     2701 gcaaacccaa acggaagtaa atgaagcttg atctatgctt catttacttc attcaatcaa
     2761 acatgaaaga aaggaaaatt ctaatgacta aaaataaaat gacactaaaa gcagaggttc
     2821 ttctgtatat tcaagaacat ttttcaaatc aagccttttt tacaaaacca atttacttgg
     2881 atttcgaaat aagaggggta tctgctggat ctatcggggg tactcttcaa gcgttgaaga
     2941 acgaaggata tttagaaaat cactttgtgc aacgttcgtt caatagaaga gtcgtgaaaa
     3001 aatggtattt agttcattca tagagagggg aatcaagcat gcgtgtggct tgttatgatc
     3061 gaacgtctgt ttatgaagaa gcgaaaatta gtaatgacgc tcaatatgat gcagtgaaag
     3121 agtatgtttt agagaaatat gaacaaattc cccaacgatt ttcagacaaa ggagaatcag
     3181 gggaaaaagc ggagcgtcca gcttttcgac agcttagagc ttgggtttgt gcgtcagaag
     3241 aagagggggt gatctatgtt tggcgttatg atcgattggc acgtcaagcc aaaatagcct
     3301 tagaatttat tgagttgtgc caacaacatc aggttcaagt gtattcgatt tctgaacctc
     3361 ttcctgcttc tttctcacac gcgtcaacga ataaactatt tgtgcaatta ttgttcactt
     3421 ttgcagaaca acaacgaaac gtcattattg agaatgttcg tgccggactt tcctataaaa
     3481 agcaacaacg gcaatatttg tcttctgcgg taccatatgg gtatcgttta gtgaatggga
     3541 aaatccaaca agaagtacac gaagcaagga ttgttcgcta tatttttcaa ctttatttga
     3601 ccaaaaaata tggctacaaa aagctttgtc aacgattgac gcaacaaaag cttttcttcc
     3661 gcgaacgtcc ttttcagccc tatcatattt atagtatcct aaaaaatcca ttgtactatg
     3721 gcgaaatcaa aggaggatct ttggggaagt atctaggaac ctttgaaccg attctctcga
     3781 aagcaacctt tttacaagcc caagagattc gccaatcaag gtgtacagct aaaaaagata
     3841 cgtatctgta cttattacgt caaaaaattc ggtgtccctt ctgtggtcgt catctgagtt
     3901 cgaagtatca atggaataca aagaagacga agacgcttca ttattatcat tgtaccgacc
     3961 gaagctgcca aggaattttt cttcgggcag aaagggttga gtcagaagta ttggaaaaga
     4021 tacaggcttt ttttcaatac gaaggcgtct atcaagcctt gattgcccaa ttaacgcaac
     4081 aattagccga aaagcgaaaa gatgaaaaac gcaaagtaat ccaacaaaag aaacaagaag
     4141 cacaatggat taaggcattt gaagagggca attgttcact ggaagaactc aagcaacaac
     4201 tgcaagctct tcaaaagccg atgtctactt cttcgtttct ttcactccaa cagtgtgaag
     4261 caaaagtgaa agaatggctt caactaaaag acctctctag tcgacaatta ttatgtgact
     4321 atgtttgtcg gattgaggta acaaaggata aaacagtttg tgggatttac ttccgacaac
     4381 tgacacaaaa tattttagaa aggaacgaat gatctatgga aatgatacaa accaaacgag
     4441 tcgcattgta tattcgcgtg agtacacaag aacaagcttt agaaggacat agtattcaag
     4501 cacaagaaca aaatggacga cagtttgccg aacggatggg ctatgaagtg gtagaagttt
     4561 atgccgatga aggaatcagt gggaaatcca caaaaaatcg gttcgcctat caacgaatga
     4621 tgaacgatgc tcggaaccat cgttttgatc tactagtgat ttggaaattg acccgtctcg
     4681 gacgcaatat gttagatgtc ttacaaacag tcgaggaatt attgcgatat gacgtaggat
     4741 tacattcgat ctcggaacaa tttgacgtga ccacttcgac aggtaaattg atgttacaac
     4801 tccttgggag tttcggtgaa tttgaacgca accaaattgc ggagaatgtt caaatgtcaa
     4861 tgcaaagctt agtgaaagat aaaaagcgtt atgcaggagg ccgacgttta ggctatgtca
     4921 gtggtattga cacgaatggt caaaaacaat tggtcattga accagaagag gcgaaaattg
     4981 ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta tcgagcgatt gccaattttt
     5041 tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt ttctaccacg gcagtcaaag
     5101 atatcttacg caataaaatt tatgcagggt atttagaata tgcgcgctac atcaattggg
     5161 aaaccaaacg acggaagggg aagaatccga gaccgatttt agtgaaagga acccatgaac
     5221 cgattatttc ggaagaagat tatcaaaaag tacaagaacg ccttgcttta gaaggaaaac
     5281 aacccaaatg gaaccataca ggtgaaaatg tattgacagg cttgttacgt tgtccagaat
     5341 gtggcgcacc gatggcagcg agtaatgtca caaataccct aaaggatggc acaaagaaac
     5401 ggattcgata ttattcttgt tcagtctttc ggaataaagg agcaagtgtg tgtcatgcca
     5461 attcgattcg tgccgaacaa gcggaaacat ttgtcgccga ccgattaaag gagatcgttc
     5521 agctaccaga agttcttccc cgattggtgg cagcgttaaa tgaagaaata gtgcgacaaa
     5581 gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg aaaggaagaa ttgaagacta
     5641 aaattgaaaa atgggaagct gctttggaag atagtcctga attgtttccg atgttaaaag
     5701 accgcttaga cgaattgact gaaaaacgac gacagttgca cattcgagaa aatgaaatct
     5761 tagggatctt tcaacaagga gagccgattc aagtcaaaga tgtccaacgg atattgacta
     5821 gtttggatcg ttttcttgcc cagagtgaga agaagcaaat caaagccttg tatcgtacct
     5881 ttatagagaa aataaccttt gatcctaacc ataaggagat gttggaaatt acgatgaaat
     5941 tcacgcatgc cgtggtacaa caattgaatg agcaatatca aatagccgta tccaaaacaa
     6001 aggatacggc tatttttgtg ttaaaaacac cctttacgtt aacgatctag tctaggttat
     6061 atcaaacaac gttagagggg cgctaacgct tctctagcga tttgaaatcg ttagagaagt
     6121 agttagggag gtccttatca taaataggga tatttattaa tgaatatttg aaatataatt
     6181 gtatataaga attaaaaaga gtggggatag atatgaaaaa gatgggtaag tgtattttat
     6241 gtgaaaaata cactgaactt actaaagaac atgtaccacc taaaaaatca gggaatactg
     6301 gaggtaaaaa gaccagaact ggtaatttag atgatttcct taagagcgat tttacaaaag
     6361 gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa tgtttattat a
//
LOCUS       DAYYKC010000131         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000131 DAYYKC010000000
VERSION     DAYYKC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.133"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHBBW_002740"
     CDS             330..1175
                     /locus_tag="ACHBBW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9154362.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHBBW_002741"
     CDS             1456..2676
                     /locus_tag="ACHBBW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9154363.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHBBW_002742"
     CDS             2673..5393
                     /locus_tag="ACHBBW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154364.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHBBW_002743"
     CDS             5377..6321
                     /locus_tag="ACHBBW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9154365.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYKC010000132         6339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000132 DAYYKC010000000
VERSION     DAYYKC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.134"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHBBW_002744"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHBBW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHBBW_002745"
     CDS             complement(1878..3080)
                     /locus_tag="ACHBBW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9154366.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHBBW_002746"
     CDS             complement(3140..3343)
                     /locus_tag="ACHBBW_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9154367.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHBBW_002747"
     CDS             complement(3485..4552)
                     /locus_tag="ACHBBW_002747"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9154368.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHBBW_002748"
     CDS             complement(4610..4717)
                     /locus_tag="ACHBBW_002748"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154369.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHBBW_002749"
     CDS             4880..5692
                     /locus_tag="ACHBBW_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154370.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHBBW_002750"
     CDS             complement(6066..6257)
                     /locus_tag="ACHBBW_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154371.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1891 a         1299 c         1000 g         2149 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaa
//
LOCUS       DAYYKC010000133         6261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000133 DAYYKC010000000
VERSION     DAYYKC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.135"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..836
                     /locus_tag="ACHBBW_002751"
     CDS             <1..836
                     /locus_tag="ACHBBW_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9154372.1"
                     /translation="RMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYIDS
                     NIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSNE
                     LMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERDF
                     TYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVETI
                     EKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYYL
                     S"
     gene            1204..2628
                     /locus_tag="ACHBBW_002752"
     CDS             1204..2628
                     /locus_tag="ACHBBW_002752"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9154373.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            2714..3142
                     /locus_tag="ACHBBW_002753"
     CDS             2714..3142
                     /locus_tag="ACHBBW_002753"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM9154374.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            3230..4396
                     /locus_tag="ACHBBW_002754"
     CDS             3230..4396
                     /locus_tag="ACHBBW_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM9154375.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            4649..4792
                     /locus_tag="ACHBBW_002755"
     CDS             4649..4792
                     /locus_tag="ACHBBW_002755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154376.1"
                     /translation="MENAITVITNSFFSSIKYLKTLFVCQADQCSFRLGRTLISSQVM
                     IEV"
     gene            5612..5731
                     /locus_tag="ACHBBW_002756"
                     /pseudo
     CDS             5612..5731
                     /locus_tag="ACHBBW_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(5779..6012)
                     /locus_tag="ACHBBW_002757"
                     /pseudo
     CDS             complement(5779..6012)
                     /locus_tag="ACHBBW_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2077 a          843 c         1171 g         2170 t
ORIGIN      
        1 tcagaatgga tctattagag aaagaaaaat tagattctct atttgaaagt gaaggttttg
       61 acatagtaat tcatttagct gctcaagcag gtgttagata ttctattgaa aacccttatg
      121 cttatattga ttctaatatt atcggttttc ttaatattct tgaagcctgc cgaaacttcc
      181 ctgtaaagca tctattatat gcatcatcta gttcagtata tggtgggaac acgaaagtac
      241 cgttttcaac agaagattcg gtggatcatc cagttagcct ttatgcggct accaaaaaat
      301 cgaatgaatt aatggctcat acttatagcc acctttataa aataccaaca actgggctga
      361 gatttttcac tgtctatggt ccatttgggc gaccagatat ggcctatttc tcttttacca
      421 agaatatttt atctggaaaa gaaattaaga tttttaacca tggaaagatg gaaagagatt
      481 tcacttacat cgatgatatc gtagagggaa tcactagatt aatcaataaa gtacctgaac
      541 ccaactccga ctggacagac atcgatgatt tgagtagtga aagttgggca ccatataaga
      601 tatacaatat tggaaataat aagccggttg cattgttgga gtttgttgaa acaattgaaa
      661 agtatttagg tgttaaagcg aacaagattt ttttggacat gcagccgggt gatgtcgtta
      721 gaacttatgc cgatattgaa gatttagagg aaaaaatcaa ctttaaaccg aatataacct
      781 tggatgaggg cattaaaaaa ttcgttgaat ggtatacagt gtattatttg agttaatttt
      841 gaaaggaata ttgaagatgg gtaattcata cgattttttt agctttgtta ggtattttag
      901 gaaattctac tttcaaacgt gacgaacttt ctcgccgaat agatattttc tataactaaa
      961 agaatttcta taaaacaaaa ttgtgtatca agatgtgtga actggattct gactgccaat
     1021 attgattgat gagattgaag aaatatatag ttgatcatgg atatctttat ttgaaaagat
     1081 cttagctatt cacatattca aaaaaatttt tactacgagg actcacacga tacagaggaa
     1141 tttaggtagt tctaattaaa ccagaaagtc atctatagat gtgagttgag ggaggtcata
     1201 cggttggata gtagcagtaa taaatcacat tcaatagcta aaaaagcttt tatttacgca
     1261 ataggaacat tatcatcaaa aatgatatct gtcattataa tccctatcta tgcagtatat
     1321 atttctgttg ccgatttagg aagctttgat tatcaaaata caattgctca atttctttct
     1381 ccagttattt ttggagcaat atgggaagca attttacgca tgggattagg caaagaaaaa
     1441 aatattcaga aaaaagtagt gacgaacagt atttgttttg ttatagcaaa tacagtgata
     1501 ttagccattt ttttaccaat aatatataga atgattttcc cgaatttaga gtattatcaa
     1561 ttgtttgttt ttcaattaat tttcactcct atcctaagtg tttttcaata tattgtaaga
     1621 gcactaggtg aatcaaagat ttttgtgatg agtggtataa tcgctgcggg tatcaacctg
     1681 ttactcctta ctatttttgt tgttacaatg ggtatgggga tacttggtct cttactctct
     1741 atcataattt ctaatgttat aaatttgatt tttttgattt tcagggggca gatctttcaa
     1801 tatttcgcaa cgagttcttt tgatttttta tttttaaagt ccatgctgaa atactctatc
     1861 ccactcactt tgaatctcac ttttggatgg ctaatagttg gtttttcaag attttatatt
     1921 ggaacgtaca tcggagtaac agaaaatggt attttttctt ttgcgatgaa attttcatct
     1981 ataattttaa ctcttacaag tattgttagt atgtctatga tagaggatgc ggttatcacg
     2041 tctgagaatg atgatttcat cgaaagattc gttgagaaaa atacctatat ttttaaaatg
     2101 tacattaggt tagcgactct ttctattcct atcatttgct gtgcatactc ttttataacc
     2161 aaccctgatt ataaaaaatc attagttcta gttccaatat tagttattat ggcatgtttc
     2221 agtgggatga caacaaatat cggaaatata tttgcagtat acaataaaac aaatcagtta
     2281 ttaatgattt cagtagtgtc aggtctattg agtgttgttg gcacctattt attaggctat
     2341 tcttttggag taattggtgt ctcatttgct actttgctcg gtactttagt attatttgga
     2401 cttagatatt attttggaag gaaaattgct tattatgcat ttgattggaa gcgaatatcg
     2461 ctacttttag ttctctattc cgcaatcgct tttgtatgca ttcatagagg cttctatttt
     2521 aatcttttct taacgattat ttttagtgct attttcgttt ttacgtataa acaattaatt
     2581 ataacgtatc taaaaaaatt tttcagaata aaaatggcta aaaggtgagg gtatacgaag
     2641 taagaataga atctttcgaa gaataaagag aaaactattt ggaaaaaatc tgcgagaatg
     2701 tattaatgaa ggtttgatat tcggaaaaaa acatagttta gtagaggttc ctgaatttgg
     2761 gagcgagcca tatttaattg aaatagggga taatttcaga tgttcatttg gtgttaaatt
     2821 tataacacac gatggtggag tctgggtctt aaggaagatg agttctgtga gcgagtcaac
     2881 ttcaaaaata ggtaggatca aattgggaaa taatattttt attggtattt actctattgt
     2941 tttcccagga gtttcaattg gcgataatgt aattattaga gcgaacactt tagtaaataa
     3001 agatatccca aataatagtg ttgtagcggg tagttcatgt aaagtgattt caactataga
     3061 ggagcattat gagaaaaata aaaatgaatt tattaaatat cgaacctcga tgattttgaa
     3121 gcaaaaaaat tttttctatt aaataatatt gattgaataa ggaatatgtt caaaaggata
     3181 acagctaaaa tactataaaa tgaaatttga ttggagcgta tggaagaata tgaaaatttc
     3241 agttttcgga ttaggctatg ttggtctagc aaattcacta ttattagcac aaaaagagca
     3301 ggtcaaagct tatgatatcg tggaagaaaa aatcacgatg ttacaaaatg gcatctcacc
     3361 aatagaagat aaagaagttc atgaattttt aaaacgggat gacttaaatg tcgaatttac
     3421 gtcaaatttt actgatgcag taaacttcgg tgattacttg attattgcaa ctccaacaga
     3481 ttatgacgaa aagaaaaact attttaatac atcaacagta gaagatgtaa ttgagaaggc
     3541 tttagcaatt cgtccagatt caacattcat tattaagtcg acagttccag ttggctatgc
     3601 aaatgaaatt aaagcgaaat ttggtacaga aaacatcatt ttttcacctg aatttttgcg
     3661 tgaaggaaaa gctttgtacg acaacttata tccatcaaga attatagttg gagaatgttc
     3721 tgctcgtggt cgcgagattg caactatgtt tcaaagaaac gcatttaaca aagatgcatc
     3781 agttcttttg actcactcaa ctgaagcaga agctattaaa ttattctcga atacatattt
     3841 ggcattgcga gtggcgtatt tcaatgaatt agatacgtat gcagaatttc gtgggcttga
     3901 tagtaagcaa attattgaag gtgtcggttt ggatccacgg atcggttcgc attataataa
     3961 tccttctttt ggctatggtg gatactgttt acccaaagac acaaaacaat taagagccaa
     4021 ctatgaaaaa gtaccaagca atatcatcag tgcaattgtt gatgcaaata caacaaggaa
     4081 agattttatt gctggacaga ttttggcgaa aaaaccaaat gttgttggaa tctatcgttt
     4141 aacgatgaag gccggttcag ataattttcg atattcgtcg atccaaggaa ttatgaaacg
     4201 gattaaagcc aaaggaattg aagtggttgt ttacgagcct acattggatg aagatgaatt
     4261 ttatcattca caagtattga aagatattaa tgaatttaaa aatatttctg atgtgatcgt
     4321 atctaatcgg tttgaagata ttttaaagga tgttgaagaa aaagtttata ccagagatat
     4381 ctttggtgat aactaaataa taatgaacgg gtacatggag gaaaatattt tagatggtga
     4441 ctcgaaataa atgtttgttc tcattgcatc tgtatctata ccgataagtt taaaaattcc
     4501 gattaggaaa atgaagttgg ctatccttgc gaggaattta ttgctggatt attttatgaa
     4561 ctaaagcaag acatcatcag aaaaaaatcc aaaatttgac aatgggattg atcgtttacc
     4621 agctgtaagc gaagtatctg acacgaaaat ggaaaatgca attactgtta ttaccaatag
     4681 ctttttttca tcgatcaagt atttgaaaac attatttgtt tgccaagctg atcaatgttc
     4741 tttcagactt ggaagaacat tgatcagctc acaagtaatg attgaagttt aggttatggg
     4801 ataaaggata agtgaaataa aagtcgtgtc tgcatctttg ttagtaccag taaatttttc
     4861 tactacggcg ctagtcacga cagtccaggc atctgaaatt gaagatgcaa atagagtttc
     4921 tgctagttta ggtattgatg ttactgtatt agcattaatc ttagagccag tagcatccaa
     4981 taggtgtagc tatagttcta atagtataaa acaatgcaga ggatatgatt gctttatgtc
     5041 tcggtgtgtc aggtcaaaat agagttccat atggggcgat ttctcaatga ataatcttat
     5101 agatataatc ttgtttgcta tttcgttatc acttttactt ttcttcgatt atttcaaaaa
     5161 taaaaatcca aaatatttta agttcttact atttatcatt actgtactag ctgttttatc
     5221 gatgacagat tttatttaac ttatctcaaa aagtagggaa ccatatcatt tttcagtaac
     5281 atgcgtatta tttgtataaa taatacaaaa tgaaaaatta tgattattct attgtctttt
     5341 tagaaagcgt tttctgtgag cttttaatct aaaaggaggg atataaaata aagaaaacct
     5401 attttatgct atttttttgt tgtttctgtg taggaatcct gtggtatata ggtggtaatg
     5461 aaaagaagat tcatggagtt gtgacaaata tttctgcgga tgagttcgta tttgaagatg
     5521 aaaaacagaa cgaatatagt attcaaaaaa gaagctcttt gtcaaatgat gttggtaaag
     5581 agaaatctta gaataatttg gacagaagag gctgggacag aagtgtttaa ctccgagaaa
     5641 taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttccgaa
     5701 ggagttgctt ctgctcccgc cgtttactcg agagtctgta gcaaaactga tttttagatt
     5761 tatcccacat tcttctgttt atttggttaa aaatgttttg ccatgacgaa tcatgatccg
     5821 tagtttgaaa ttgtaaaagc tgcgaaagcc atacgtattg cgttttaata ctttgatgtg
     5881 gttgtttaaa cattccaatg gtctgtttga atagctgcag ttaagcgcat tttgaatctg
     5941 tggcagaaaa tttttaaacg ttcgtaaagt ggttgtatag gtttcaggca gatcgctaat
     6001 gtctctagtt aaacagtcct caaatagtga cgaatctctt ttctttcact caatgattca
     6061 agaaggttct tttaatatat tggaagatgt ggatattctt ctggtgtgta ctgaagagtc
     6121 cctttgtaaa tcaagcaaat ttgtcctttg tatttttctt tagttaaaca attttcctcg
     6181 aaatggatat tgatatttag gtctagaata tttagggtat ctttgataag cagagtatga
     6241 gacatcttga atcctcctat g
//
LOCUS       DAYYKC010000134         6251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000134 DAYYKC010000000
VERSION     DAYYKC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.136"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBBW_002758"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBBW_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9154377.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHBBW_002759"
     CDS             499..861
                     /locus_tag="ACHBBW_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9154378.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHBBW_002760"
     CDS             913..2595
                     /locus_tag="ACHBBW_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9154379.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHBBW_002761"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHBBW_002761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9154380.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBBW_002762"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBBW_002762"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9154381.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBBW_002763"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBBW_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9154382.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2075 a         1089 c         1427 g         1660 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaaggatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt t
//
LOCUS       DAYYKC010000135         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000135 DAYYKC010000000
VERSION     DAYYKC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.137"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBBW_002764"
     CDS             725..811
                     /locus_tag="ACHBBW_002764"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9154383.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHBBW_002765"
     CDS             complement(932..1621)
                     /locus_tag="ACHBBW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154384.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHBBW_002766"
     CDS             complement(1628..1906)
                     /locus_tag="ACHBBW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154385.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHBBW_002767"
     CDS             complement(1866..2780)
                     /locus_tag="ACHBBW_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9154386.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHBBW_002768"
     CDS             complement(2762..3142)
                     /locus_tag="ACHBBW_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9154387.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHBBW_002769"
     CDS             complement(3592..3879)
                     /locus_tag="ACHBBW_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9154388.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHBBW_002770"
     CDS             complement(3899..4123)
                     /locus_tag="ACHBBW_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9154389.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="ACHBBW_002771"
     CDS             complement(4578..4793)
                     /locus_tag="ACHBBW_002771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154390.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHBBW_002772"
     CDS             complement(4763..5296)
                     /locus_tag="ACHBBW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9154391.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHBBW_002773"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHBBW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9154392.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYKC010000136         5835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000136 DAYYKC010000000
VERSION     DAYYKC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.139"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHBBW_002774"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHBBW_002774"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9154393.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBBW_002775"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBBW_002775"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9154394.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHBBW_002776"
     CDS             2594..3379
                     /locus_tag="ACHBBW_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9154395.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHBBW_002777"
     CDS             3392..4243
                     /locus_tag="ACHBBW_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9154396.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHBBW_002778"
     CDS             complement(4367..4819)
                     /locus_tag="ACHBBW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154397.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ACHBBW_002779"
     CDS             complement(5284..5652)
                     /locus_tag="ACHBBW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9154398.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1981 a          940 c         1177 g         1737 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaa
//
LOCUS       DAYYKC010000137         5834 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000137 DAYYKC010000000
VERSION     DAYYKC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.140"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..1076
                     /locus_tag="ACHBBW_002780"
     CDS             180..1076
                     /locus_tag="ACHBBW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9154399.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1226..2167)
                     /locus_tag="ACHBBW_002781"
     CDS             complement(1226..2167)
                     /locus_tag="ACHBBW_002781"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9154400.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2293..3414
                     /locus_tag="ACHBBW_002782"
     CDS             2293..3414
                     /locus_tag="ACHBBW_002782"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9154401.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3672..5174
                     /gene="eat(A)"
                     /locus_tag="ACHBBW_002783"
     CDS             3672..5174
                     /gene="eat(A)"
                     /locus_tag="ACHBBW_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9154402.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5241..5768
                     /locus_tag="ACHBBW_002784"
     CDS             5241..5768
                     /locus_tag="ACHBBW_002784"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9154403.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1913 a         1031 c         1224 g         1666 t
ORIGIN      
        1 agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt
       61 catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct
      121 ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga
      181 tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca
      241 tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag
      301 ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg
      361 agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc
      421 acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg
      481 agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat
      541 tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg
      601 caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag
      661 atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta
      721 cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg
      781 agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag
      841 tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc
      901 ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc
      961 tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg
     1021 attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa
     1081 cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga
     1141 gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt
     1201 cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga
     1261 ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc
     1321 aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt
     1381 acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt
     1441 acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata
     1501 cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat
     1561 ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat
     1621 aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt
     1681 tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta
     1741 acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga
     1801 tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac
     1861 acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt
     1921 gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt
     1981 ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc
     2041 catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc
     2101 cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga
     2161 ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga
     2221 agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag
     2281 tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca
     2341 tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat
     2401 gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga
     2461 acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta
     2521 ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta
     2581 gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga
     2641 gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga
     2701 agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg
     2761 tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt
     2821 cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa
     2881 caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc
     2941 ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta
     3001 gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga
     3061 gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc
     3121 gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga
     3181 aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt
     3241 tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt
     3301 attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa
     3361 acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca
     3421 aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca
     3481 ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga
     3541 agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg
     3601 tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga
     3661 ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag
     3721 gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta
     3781 tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt
     3841 atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata
     3901 aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg
     3961 aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac
     4021 tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt
     4081 tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg
     4141 cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg
     4201 atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga
     4261 atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg
     4321 aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt
     4381 ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta
     4441 gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag
     4501 caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg
     4561 aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg
     4621 tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg
     4681 agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc
     4741 attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc
     4801 tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg
     4861 cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg
     4921 aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg
     4981 aattggctaa atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt
     5041 atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca
     5101 tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc
     5161 ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg
     5221 ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg
     5281 cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg
     5341 cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt
     5401 cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc
     5461 tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca
     5521 aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt
     5581 cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag
     5641 aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc
     5701 atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt
     5761 atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta
     5821 gcagtgcctt tttt
//
LOCUS       DAYYKC010000138         5779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000138 DAYYKC010000000
VERSION     DAYYKC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.142"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHBBW_002785"
     CDS             <1..813
                     /locus_tag="ACHBBW_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM9154404.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHBBW_002786"
     CDS             786..1229
                     /locus_tag="ACHBBW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154405.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACHBBW_002787"
     CDS             2363..3331
                     /locus_tag="ACHBBW_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9154406.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACHBBW_002788"
     CDS             3341..3829
                     /locus_tag="ACHBBW_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9154407.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACHBBW_002789"
     CDS             4125..4406
                     /locus_tag="ACHBBW_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154408.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACHBBW_002790"
     CDS             4408..4824
                     /locus_tag="ACHBBW_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154409.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5492..>5779
                     /locus_tag="ACHBBW_002791"
     CDS             5492..>5779
                     /locus_tag="ACHBBW_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM9154410.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DAYYKC010000139         5767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000139 DAYYKC010000000
VERSION     DAYYKC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.143"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(394..897)
                     /locus_tag="ACHBBW_002792"
     CDS             complement(394..897)
                     /locus_tag="ACHBBW_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9154411.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1256..2281
                     /locus_tag="ACHBBW_002793"
     CDS             1256..2281
                     /locus_tag="ACHBBW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9154412.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2604..3482
                     /locus_tag="ACHBBW_002794"
                     /pseudo
     CDS             2604..3482
                     /locus_tag="ACHBBW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3958..4074
                     /locus_tag="ACHBBW_002795"
     CDS             3958..4074
                     /locus_tag="ACHBBW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9154413.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5002..5340
                     /locus_tag="ACHBBW_002796"
     CDS             5002..5340
                     /locus_tag="ACHBBW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154414.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5434..5664
                     /locus_tag="ACHBBW_002797"
     CDS             5434..5664
                     /locus_tag="ACHBBW_002797"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9154415.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1961 a          863 c         1068 g         1875 t
ORIGIN      
        1 taggatcagg cagaatatac tggcagaatc aaggagaagg gagcctaaat aaggagaaaa
       61 accatagaaa atataaagta attacttata aaacaagctt tttatagaaa aataatatac
      121 ctgagtaggg tataaattaa gacggaattc ctttctagat tgagatattg actaggaaga
      181 atgttaagat tttaagcctt taagctaaaa aaaaaccctt tttgttaaat aaggggaata
      241 tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag attagagatg
      301 aaaatagatt tttttaaacc aatgactgaa taaaaagacc gtcatagata cacattggaa
      361 tgtgtctatt acggtctttt tttacgaacg attttattga agacggacag ttcgggtcaa
      421 aaataaaaaa gctacgatca gaaataagaa taaaatgtat cccaaaataa tggctagacg
      481 gtctaactta tttgtcgata gatgtttgtt tagaagttga taggcccata aacctatcag
      541 tcctcctgtt gtattagtta tcaaatcagt gatgtccgct acgccaatac cgaataggaa
      601 ttgaatcaat tctgccaaga agcttacaag gaaaataatc cctaattttt tagaaaaaga
      661 aagtctcttg aaattgacat tcaaaagcac cccaaaagga aaaaagacaa tcaagttata
      721 agtggtctca cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt
      781 gacattgctg taagaataag tgagaaattg tggaagatta aatgatagct tgaataaaat
      841 caaccataat agaaagaata aataaatccc taataaaaat tttgatacat accgcataga
      901 catctcctcc atttaaaaag tataccatcg aaaaccgctg aaaaatcata aaaaagtatg
      961 aagtttaagg accaacgtag ttatcgtacg aaaattagaa gctttttttt gttgggaatc
     1021 cggtttcttt aacaaagcta agatagcgag ttgcttggaa aagttgctat cttttttctg
     1081 tcaaaacgac tcggaatgtt cgttttttgt tctaatttct ccaagttgtt tactttttga
     1141 tactaagttt atgaaaaatc aagaaatagt tgttatacta ggtaatgctg acaaacggga
     1201 ttggcatttc atcttggttc agcttttttt ccaattacta ggaaggaggg atagattgtt
     1261 caagacttat tttcgtttca ataaaataca agataatcct tttcgtatca tgaatctgat
     1321 gctacttatc tttattgtat tatggttagg ttacataaat catgattttt tgatttcaag
     1381 tttttcatca ttagggatct ttactttttt ctattatcaa aacataccga tgaaatattt
     1441 gctgaaacgt atgtttctga tcggttgcgg actgttggtt gctttcgttt tggggatctt
     1501 atcgacttat gttttatggc ttgaaccatt tgccgtagct ttggttgctt tttccagcag
     1561 attcgtgttg cgattattcc atatttctaa accaggtggc ttgttttttg cgatgctttc
     1621 tgccatggga acgagcatgc agctgccaat cgctcaatta ccgatcgtct cactttattt
     1681 ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt acaaaattac tggattcacg
     1741 gcctgagcag acaattgaaa aagcaacttt aaaagaaaga tttcacgaag agcctttagt
     1801 gatcattgat tctgttttct acagcgctgc cttatttctt tccgtttatg tcagtcacgg
     1861 cttgaatctg cataatcctt attggttgac cttgtcatgt gcatcgattt tgctagcaga
     1921 aaacttggat gcgatgaagc atcgacaagt ccaatatctg attggatcga tgggtgggtt
     1981 atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag ttgcagacca tttttctgat
     2041 cacttttcta tatggaatcg cacaatttct agttgctaga aattatgcag ttgctaatat
     2101 ttttcttaat ccgatggcgt tgatgctatc tacacttatt cgtggagcat acttgatttc
     2161 tttgatcgaa tatcgttttt tagggatcgt cataggaagt ttcatcggtt taggtgttgc
     2221 gtgggtaatg acggttggtt tgcaacatta tttaacggtt gttcgtcaaa aattagaata
     2281 gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca acctctaatt
     2341 caaggtgttc aaaagccaga ttctcagcat taagtcaaat tcacacaaaa atgtggaaag
     2401 gaactttcaa aaaaatttta tatttattct atcaaaaaat tttgtttatg atggtaactg
     2461 aataaacttt cctgagatgg tcaatgcttt ccttgaaagt cttacagata ttctttcttc
     2521 atagaaagtt taagttttat agttagtgta caataaaagt atgtttatac catatggtgt
     2581 ccggataatc agtcatttaa acaatgaggt tcaaagatat gaaaagggga aatttgatga
     2641 agaaaataag caatgaaatc caaattctgg cagaaacttt aactcttccc aaagtcgtta
     2701 tagagcaaat gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag caatatagtc
     2761 atgaactaat ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg
     2821 aagaggaacc ttttgcagca ttagctattt atctatatgc agcttctcaa agttggaaaa
     2881 aaatttatca accattgaag attccaagaa ctatctattt ggcaacaatg aatgctttta
     2941 ctagatttat tcatgaacat ttccaagtaa caggaagcta tcgctttgac cgtggttttt
     3001 ggatttggca ttacactagt ggcttgatct ttcggattgg tgaattggag tacgaaatga
     3061 catattttcc gaaagggtac acggatctaa gattagaagg aaagcagtgc ctttctcttc
     3121 atattccttc cgatgcagat ttatctcaga ataagataag agatagttac gaaagtgcca
     3181 ttcgtttttt tgaactttat tttcctgatt accattacca agtgatgtac acagatactt
     3241 ggttactatc acccaatctt actaaatagt tgaaaaaaga gtcaaaaatc tgtctttttg
     3301 cagccgatta tcgattatta tcagttgatg aacaggacga tagtggagtc ccttggatct
     3361 ttggacgagt agatgcccaa atacatgatt atccagaaag tacgagttta cagcgacaag
     3421 caaaagagca attgcttgca ggcgagcaca ttggctctgg cttaggtatt cttacttcat
     3481 aagtttagta cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt
     3541 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
     3601 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     3661 cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt tcaataggta
     3721 aactagctat aacaatagat attaaagtta acggacaaga gggccttttt taggtagcta
     3781 aaacgtaata atgaaaattt atttccagag ataggagtca atcagctagt ctgatcgact
     3841 gttttaacaa gttgactttc attttaactt ttgataccat ttaaaacaca aatcgtaatt
     3901 gtttcgattt gtgtttttta gcatgactac aatcaaagaa gaaagtgggt cgtccatatg
     3961 gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa gcccaaatat caaaacacga
     4021 aaacgtgcgc tgaagatcat caaagatgtg aaacggaata gtgggaaaag gtaagaagaa
     4081 ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa taatcggttt
     4141 ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata tgataaattg
     4201 gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata gttaactaca
     4261 gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc aattcccttt
     4321 caacctctta ttttgaccag tagtattgat cactttacct tttaacacct ttgttcgttt
     4381 ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt ttgcgaaata
     4441 tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca ttatcgttta
     4501 ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat ttcttttctt
     4561 caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact ttaaaaagta
     4621 acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa taacatcaag
     4681 gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga attttagata
     4741 cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt atctttaata
     4801 aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt agtcagtcga
     4861 ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt ggaagataaa
     4921 aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat tttccctctg
     4981 tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca tgataacctt
     5041 tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga gactccatta
     5101 gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat acagtacgaa
     5161 gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga gctaacaccg
     5221 attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga cgagactgag
     5281 atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt gtggatgtag
     5341 acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat gtagcacaag
     5401 aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg aaaagaaaat
     5461 atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa agaattcaag
     5521 agtcgctact caattaactc gactccgaca tttacagttt acagagaagg atcgatagaa
     5581 aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga agagtttttg
     5641 gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg acacatatta
     5701 tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc agtataaaga
     5761 gtatcca
//
LOCUS       DAYYKC010000140         5573 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000140 DAYYKC010000000
VERSION     DAYYKC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.144"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      108..274
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            422..979
                     /locus_tag="ACHBBW_002798"
                     /pseudo
     CDS             422..979
                     /locus_tag="ACHBBW_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1037..3076
                     /locus_tag="ACHBBW_002799"
     CDS             1037..3076
                     /locus_tag="ACHBBW_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9154416.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3185..4336)
                     /locus_tag="ACHBBW_002800"
     CDS             complement(3185..4336)
                     /locus_tag="ACHBBW_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9154417.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4490..5020)
                     /locus_tag="ACHBBW_002801"
     CDS             complement(4490..5020)
                     /locus_tag="ACHBBW_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9154418.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5119..5469)
                     /locus_tag="ACHBBW_002802"
     CDS             complement(5119..5469)
                     /locus_tag="ACHBBW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9154419.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2022 a         1022 c         1042 g         1487 t
ORIGIN      
        1 tttttttcta aaaaatatta aagattgtta aatttcacta tttcatttca aattccattg
       61 cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag aatatagtaa taaccttgtt
      121 aggtgaggct cctatacaaa cataggctgt tgcccagaaa cgtcgaaagt cgccaatggg
      181 taaaacagaa actgttggat taaggctttt tccaatagct aagaatgttt tctttacgtt
      241 gtatagtgct aaaactcgga cgatgagatg atgctaggtc agaattcttg ttcgaaaagg
      301 tttctgacct tttctttata tttttgtgac tgacttattt gatgagacaa actcgctcct
      361 cacctgacaa gtggattgct gtgtgaggag gagatgcggt gaaaaaagat cgattggtgg
      421 aggtcgctcg gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact
      481 gatctctatc atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa
      541 tgcagcttac tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa
      601 atttgaagct ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga
      661 actgtttcct ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata
      721 tataaacaaa ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg
      781 ggaagacaaa gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat
      841 ctctcagcag ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt
      901 acttggtagt atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt
      961 cctcaaagac atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca
     1021 agagaaggag gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta
     1081 tattgccggc gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca
     1141 ctatcaagaa acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc
     1201 tttgaacaag ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa
     1261 aagtgcatta tctgaaaaag agatcttaga tcaataccag aatatatacg gtgctgctga
     1321 tgtcaaagga cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag
     1381 tttttcatat aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa
     1441 aggaacatta gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt
     1501 tccagaaatg gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa
     1561 tattatagat cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt
     1621 cgttccaagt aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc
     1681 ctccttcgac ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc
     1741 cgattacttt gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc
     1801 taccattcaa gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat
     1861 tggttacgtg ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa
     1921 tggaaaaatc ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac
     1981 aggtgagaaa ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca
     2041 tgaagttcaa aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc
     2101 ttttgacagt ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga
     2161 tcttcttgcg cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc
     2221 acaagaagat tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc
     2281 gacaggttat gctcctggat ctacgtttaa aataattaca gcagcaatcg gtctcgacaa
     2341 cggcactatc gatccaaatg aagtgttgac gatcaacggg cttaaatggc aaaaagacag
     2401 ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat gtatcacaag tagacttaaa
     2461 aactgctttg atctattccg ataatatata tgcggcacaa gaaacgttga aaatgggtga
     2521 gaaaaaattt cgtacaggct tagataaatt catttttggt gaagaccttg atttgccaat
     2581 tagtatgaat ccagcacaaa tttctaatga agatagcttt aactcagata tcttgctagc
     2641 tgatactgga tatggacagg gcgaacttct aattactcct atccagcaag cagcaatgta
     2701 ttctgttttt accaacaatg gcacacttgt ctatcctaaa ttgattgcag ataaagagac
     2761 aaaagataag aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag
     2821 agaagttgtg caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt
     2881 ggcagcgaaa actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa
     2941 cagtttcttg tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga
     3001 aaataaagat gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa
     3061 ccaaaattat caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt
     3121 cacagtcctt ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat
     3181 ttactcacac ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg
     3241 tacttaataa aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt
     3301 gctgtccaat ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga
     3361 ccatcaatga actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca
     3421 atttggtcaa aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt
     3481 cagaaaaatc aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc
     3541 ctaaagcctc tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc
     3601 cagcaaaaaa agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc
     3661 ctaaacaaat aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa
     3721 atgaaagtag gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta
     3781 gctgttcaat caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag
     3841 ataggctgaa acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa
     3901 ttgttgatcc ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac
     3961 tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga
     4021 atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga
     4081 acataccagg tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa
     4141 acattaataa gaacacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc
     4201 aattcaatcc agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga
     4261 tacctaatct ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa
     4321 taccaataaa ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa
     4381 catacattat tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt
     4441 tttttggatt gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag
     4501 tcatttttct tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa
     4561 agccgaatat ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc
     4621 cgccaatcaa caacagaata ctactataaa cactccaact tttagctttt gtattcatgc
     4681 tgaagaacaa ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga
     4741 tagaaacgat tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac
     4801 cactgaacag accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa
     4861 gattcaacaa cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa
     4921 cagcagaagg gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata
     4981 aggcgtattt ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac
     5041 ttattttaac gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga
     5101 agaatatctc tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag
     5161 ctcatcgatc attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc
     5221 tactcgtaca ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc
     5281 ttccgcaaaa aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg
     5341 ggaataaccc gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct
     5401 ctccgctttt gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt
     5461 ttgcgccata atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag
     5521 aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaa
//
LOCUS       DAYYKC010000141         5384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000141 DAYYKC010000000
VERSION     DAYYKC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.145"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..357
                     /locus_tag="ACHBBW_002803"
                     /pseudo
     CDS             8..357
                     /locus_tag="ACHBBW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(629..919)
                     /locus_tag="ACHBBW_002804"
     CDS             complement(629..919)
                     /locus_tag="ACHBBW_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154420.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1321..1557
                     /locus_tag="ACHBBW_002805"
     CDS             1321..1557
                     /locus_tag="ACHBBW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154421.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1564..3516
                     /locus_tag="ACHBBW_002806"
     CDS             1564..3516
                     /locus_tag="ACHBBW_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9154422.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3588..4085)
                     /gene="dfrG"
                     /locus_tag="ACHBBW_002807"
     CDS             complement(3588..4085)
                     /gene="dfrG"
                     /locus_tag="ACHBBW_002807"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9154423.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4412..5104)
                     /locus_tag="ACHBBW_002808"
     CDS             complement(4412..5104)
                     /locus_tag="ACHBBW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9154424.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1755 a          930 c          877 g         1822 t
ORIGIN      
        1 tacttaattg ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa
       61 ttttcattat atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag
      121 aattcgtcga ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa
      181 gatatagatt cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt
      241 tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc
      301 gagttacata ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac
      361 tagtaatgac tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg
      421 gcttcgaatt gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta
      481 gtgattcatc acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc
      541 gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta
      601 ggacattttt gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt
      661 ttcttgggta tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat
      721 aggatctcac aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc
      781 cttcgtctac ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat
      841 ttaaactgaa acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg
      901 ttccatattt tttctccata accttcacct caataccatt atacgaacat acattccctt
      961 tgtaaagaag aaactctgtt ttatcttttt gactttttgt ctattacctt tcttaaaatc
     1021 atattggcac ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc agcagtatag
     1081 atttaagtag ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa
     1141 attgcaactg aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac
     1201 accacaaaat gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga
     1261 atgcaaattg gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct
     1321 atgcaatttc ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag
     1381 caagtaaggg tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca
     1441 gtggatgaat tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac
     1501 aagcgaagta ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag
     1561 acaatgacca agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt
     1621 ccgatttcaa acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa
     1681 cgatttgcaa caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt
     1741 tctgaatatc ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa
     1801 gaacagcaca aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt
     1861 tccaaagata aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta
     1921 tcgcaaaact gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc
     1981 cttatagaaa tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc
     2041 tgctcggaag aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa
     2101 agtaggggca aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta
     2161 ccaaaaagag aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc
     2221 gctaagatgg ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga
     2281 gttggaacct actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa
     2341 cgatatgaga cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac
     2401 aagatgcact actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg
     2461 gggtttgaag accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga
     2521 tatgtgttcc gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa
     2581 gatacaagaa aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta
     2641 gattggtctt attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat
     2701 cttcacgaat tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat
     2761 gctccttata ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa
     2821 tggcgaatga ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg
     2881 aaagaattaa ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc
     2941 agaaaacaat gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat
     3001 atcagaggat tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta
     3061 ctaacaagtc atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa
     3121 tatgccgata accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt
     3181 gattgtagaa cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat
     3241 gttaatgacc attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt
     3301 gaaagaccat taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat
     3361 ccgaagtatg ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct
     3421 tttaaatcag cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa
     3481 caatttgatt ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca
     3541 agctcttgtg ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag
     3601 ttttttcttt catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct
     3661 ttttgggcaa atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct
     3721 tcgaattcat gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta
     3781 taaatctgtt ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca
     3841 tcttctattg aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga
     3901 ataatatttc ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat
     3961 attatcggat gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa
     4021 ggaatgtcat tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact
     4081 ttcatttata acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa
     4141 aaccttttct cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt
     4201 atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata
     4261 aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag
     4321 tacattttgt cattttgtac tccaaaactg aactacttag tatagattac tgttgcttct
     4381 ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt
     4441 gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat
     4501 attgtttcca gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta
     4561 actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat
     4621 ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat
     4681 ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga
     4741 tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt
     4801 gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt
     4861 cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa
     4921 gatctgtaat cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat
     4981 tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc
     5041 ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt
     5101 tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca
     5161 caatgtcaag cgtttttaaa ttacatatgt aattttaata taaatactat caaatactca
     5221 tttaatcctc cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa
     5281 tactttctta aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt
     5341 aacccttttc atttcataga ttactgtatt tattacaaaa aacg
//
LOCUS       DAYYKC010000142         5376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000142 DAYYKC010000000
VERSION     DAYYKC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.146"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..867
                     /locus_tag="ACHBBW_002809"
     CDS             76..867
                     /locus_tag="ACHBBW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9154425.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            833..1786
                     /locus_tag="ACHBBW_002810"
     CDS             833..1786
                     /locus_tag="ACHBBW_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM9154426.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2184..3158)
                     /locus_tag="ACHBBW_002811"
     CDS             complement(2184..3158)
                     /locus_tag="ACHBBW_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9154427.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3273..3707
                     /locus_tag="ACHBBW_002812"
     CDS             3273..3707
                     /locus_tag="ACHBBW_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9154428.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3832..4854)
                     /locus_tag="ACHBBW_002813"
     CDS             complement(3832..4854)
                     /locus_tag="ACHBBW_002813"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM9154429.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5246..>5376
                     /locus_tag="ACHBBW_002814"
     CDS             5246..>5376
                     /locus_tag="ACHBBW_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM9154430.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGL"
BASE COUNT         1705 a         1021 c          956 g         1694 t
ORIGIN      
        1 aaaatcgttt atctatataa ttaagaagaa caaagtcgaa caaaaattga cttttatgtt
       61 aaaggagtaa ttcatatgct gaaaaaagaa agacaagaac aaatcattac attattagag
      121 aaagattttt atttaactaa ttcagaaatc gctaacaaat taggcgtatc agaaatgacc
      181 atacgccgag atgtcactga attagcaaaa aaaaataaag taaaaaagct atatggtggc
      241 gttgaacgaa aggaacctta taatcgagaa ctatcgactc aagaaaagat tgatacgaat
      301 gtagccgaaa aaaaatatat tggtcaagtc atgaacagta tgattcccga taattctact
      361 atttatgtcg gtgctggaac aacgatccta tatgcacttc ctttcattca aaagaaaaat
      421 ctatttgtaa ttacaaacag tttgattgct ttcaactatt taaagaatca tacaagttat
      481 cggattttac ttactggtgg cgaattttct cctattacag aagagttcat tggggaagtt
      541 gcagaaagat cttttgaaag tttgaatgtc gatatctctt tcgctgcaac aaacggcata
      601 tttgataaca atgtgacaac cgctcaattt attgagggaa atattcagaa cgcagcgctc
      661 aatgcagcaa aaatcaaatg cattgttgca gattattcaa aattaaataa atcagacgtc
      721 tatactttta gaaaattaac cgattttgat tatctaataa ccgataatca tttatcagaa
      781 caggatttca atcattattc aaaatactta acaatattaa aggagcccaa aaatgatact
      841 gacaatcacg atgaacccat ccattgattc catttatttt acaaaacact ttgaattagg
      901 tacgatgaca cgctttgatt ctccggtacg ctatgtaggc ggcaagggaa tcaattgcgg
      961 acgagcttct gctctactag gttcatcagt tattttaaca gggtttttgg gcggttattt
     1021 tggcagtatg attcaagata tgctgaaaaa tgaaaatttg tttgatttgc attttttacc
     1081 tattgcagaa gaatcaagag gtgccgtcac gatcatgcat gatgatgaaa cgcagacaga
     1141 gctagtggaa gctggtcctg aaatagcaga tactactatc aaaaaattaa tgaatgatat
     1201 caaactaatc agtaaaaata attacctttc agtcattaca ataaatggtt ctgttaacaa
     1261 tccagatccc tttttgtatg ctaaattatt acaatatatc aaagaacaaa tagatccttc
     1321 cacctatata ttaatggata tatctggaca acatctccgt gaagtcttag caagtaagta
     1381 taaaccgatg ttcatcaaac ctaatatcca tgagttttca gatatcatcc atcaaaaaat
     1441 tactcaaaaa agcgacataa aacaccatct aaaaacaaat ccactattta aagatatcga
     1501 tgttatcatg atttcttgcg gctcgtatgg tgcagtagtc aaagtaaaag aagaattttg
     1561 gaatgttgag atccctaaga ttcctatcat taatacaaca ggctctgggg atgccactgt
     1621 tggaggtttt gctcattcat tagaacagaa ttatccttta aaagaatgtg taaggtacgc
     1681 aatggcttgc ggcatgtcga atgcacagca atcagaagtc gggaaagtta ttccagctga
     1741 tgtaaaaaat ttagcaaaaa gcattaaaat cacagaaatg aaataaatag tacaaagtaa
     1801 ataattgttt atatgaaatg agacacaaag agactgtgac aaaaaacgtg tgtaccgacc
     1861 cccaaaagtt agattttttg gtctaacttt tgggagtcga tacattgtca cagtcctttt
     1921 ttcgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
     1981 atgagaagct attttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacga
     2041 cctcttggta taaacggtgt tcaaaagctt gcatctacgc taatgagtcc aaccaagtca
     2101 aaaatgagga gcttttttgg ctgtccttat tacttgatgc agggcagttt tttcataatc
     2161 tcttttagat taaatccttt tggttacaca aggttctctt caaagaatgt tgccattttt
     2221 tcaaatggaa tgatgtctgt ctggtcgtat agatctgtat gggatgctcc tggaatgatc
     2281 atgagttcct tgttgtcacc ttttagttct ttgaaagcgt cctccccaaa gtaacgtgaa
     2341 tgagcttttt ccccgtgaat gactaagact gcatgacgga tttctggaat ataatgcaat
     2401 agttttgcat tcataagtga caaccctgat gtcctattcc aaccatcatt ggagtttaaa
     2461 gaacggacat gatatccgcg gtctgtttta taatacgcat gatagtcttt gacaaattga
     2521 ggagcatctt caggcaacgg atcaaccaca cctccagcac gtgcgtagtc cccttgtttg
     2581 aaatcaatga tgcgttgtgc atttaattct tcacgcattt catatcgggc attttcatcc
     2641 ataacatcga agtagccatt tgctgttact cgagtcatgt catacattgt ggaaacaact
     2701 gttgctttga cccgcgtatc gatagctgct gcattcaatg ccatacctcc ccatccacaa
     2761 ataccaatga ttccgattct ctcagggttt acattttttt gtacagataa aaagtctact
     2821 gccgcttgga aatcttccgt attgatatca ggcgaagcaa catatcgagg tactcctccg
     2881 ctttctcctg taaacgatgg atcaaacgca atcgttaaaa agcctctttc agccatcgtc
     2941 tgcgcatata gaccagacga ttgttccttc actgcaccaa atggcccgct cacagcgatt
     3001 gccggcaatt tcttttcact tacttctttt ggttcatata aatcagcgac taatgtgata
     3061 ccaaaacgat tcgggaagct cacctttcga tgattgactt gattgctttt tggaaatact
     3121 ttgtcccatt cttgtgtgat gtttaattct ttattcatat tctagactct cctttgatca
     3181 aaattgacat ttgtttttga atgtattatt atgataaaac ttaaacttga ctttaggtca
     3241 aggtaaaaat tttattttag gaggattttt ttatgtattc cattggacaa gtgtctgaaa
     3301 tgtttggatt gcctgtttct actttacgtt attacgacaa agagggatta tttcctgata
     3361 tccagcgtgt ttctggtatt agaaaatttt cagagaatga gttagaggct ttaagagtca
     3421 ttgactgttt gaaaaaatct ggtttagaaa taaaagacat taagcaattc atggaatgga
     3481 gcaaagaagg cagtaagaca tttgaggttc gtaaagaact atttgaaaaa caaaaagaag
     3541 tagtagaaaa agaaatagca aaattagaaa gagtactcgc tatgctaaac tacaaaagct
     3601 ggtactatga agaagcaata aaagccggca atgaagaagc cgtattgaca atgattcctg
     3661 atgatttacc ccaacatgta aaagaagctt atgtacattc tcattgaaca aacggataac
     3721 tcactctcct tctctatgaa cttcatgaaa acatgaataa acacctctaa ctgattgtta
     3781 gaagtgttta ttttttcact caagtatcct gcacttacaa aaatatcctt tttaccgcca
     3841 tcctaattct ggagcgatat cttttacgat tgtttcaagc aagtgcatgt tatattctac
     3901 ccccaaagta ttaggaatcg tgacaagtac tgtgtctgct tctttgattg cttcatcttc
     3961 agctagctcc ttgattaatt gatcaggttc accagcaaaa gtttttccaa aaatcgttgg
     4021 gtttcggtga taagcaagat agccgacagc atcttgttgc ggactaccgt cttgtccaaa
     4081 taagcgtcga tccaaatcag tggtgattgg ctgaatcgag cgagtaacta atgttcttgg
     4141 ttcaaaatca tgacctgctt gcttccacgc ttctttatac gcacgtaatt ggtttgcttg
     4201 ttggatatgg aatggctcgt gggattcgta attgactaat gtagatgatt gcagattcat
     4261 tccatgttgt gctgcccaga ttgccgtttg ctgcgaacca gctccccacc agattctttg
     4321 tcgtaatcct tctgaataag gttctatccg taattttcct gatccttgtg ggaacattgg
     4381 ttgaggattt ggttcttcaa atggttttcc ttcaagtaat tccaagaatt caagggtgcg
     4441 ttcccgtgtt acctctttga tttcttcttc actgtaatca taaccaaagt atttccagcc
     4501 atctagaact tgttcaggtg aaccacgccc cactcctagt tgcactcttc cttgcgtgat
     4561 gatatctgtc aatccggcat tttctgccat ataataaggg ttttcatatc gcatatcaat
     4621 aagtcctgta ccgatttcaa tcttacttgt ctttgcgccg attgccgcta gcaaaggaaa
     4681 cggcgaccca atctgagggg caaaatgatg tacgcggaaa aacgctccgt ccactccgat
     4741 tttttctgct tccaccgcta aatcaatcga ttgcaaataa gcatcttgag ctgatttcac
     4801 aagcgatcct tgctccatcc aatgtccaaa gcttaaaaag ccgatttttt tcaaggtaat
     4861 gacctccttt aaacctacaa aaatggaagg ttttttattt ttaatatgaa ctgattctac
     4921 tacttttttt cttaaaagac gaataattgt tctcgcctca caatcaagaa atgtatcggc
     4981 ttactacacc cctactttct tcatcatctg ccgtgtctta atctctttta acagtctgtt
     5041 acatcgttga tttttgactc aaaatgattg tttctaacat gtatttatgg taaaaaagac
     5101 aagattagtt ctttgtacac atcgatttat acgctgaaca aaaagaaata tataaactaa
     5161 gaaacccatt gttttttcgc tccatcacat agagtttact ttagataaac agtgacattt
     5221 actaatccat aacggaggaa atcaaatgaa aaaaatctat ttcttatgta cgggcaattc
     5281 atgccgcagt caaatagcag aaggctacgc gcgtaaatac ttgtctcctt caatgtttga
     5341 gataagaagt gcggggatcg agacacatgg cttgaa
//
LOCUS       DAYYKC010000143         5311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000143 DAYYKC010000000
VERSION     DAYYKC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.147"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBBW_002815"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBBW_002815"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9154431.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHBBW_002816"
     CDS             complement(1297..2133)
                     /locus_tag="ACHBBW_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154432.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHBBW_002817"
     CDS             complement(2317..3534)
                     /locus_tag="ACHBBW_002817"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9154433.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHBBW_002818"
     CDS             complement(3683..3844)
                     /locus_tag="ACHBBW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154434.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHBBW_002819"
     tRNA            3917..4000
                     /locus_tag="ACHBBW_002819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACHBBW_002820"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACHBBW_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAYYKC010000144         4976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000144 DAYYKC010000000
VERSION     DAYYKC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.148"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..225
                     /locus_tag="ACHBBW_002821"
     CDS             13..225
                     /locus_tag="ACHBBW_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154435.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(202..1812)
                     /locus_tag="ACHBBW_002822"
     CDS             complement(202..1812)
                     /locus_tag="ACHBBW_002822"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9154436.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2034..2279)
                     /locus_tag="ACHBBW_002823"
     CDS             complement(2034..2279)
                     /locus_tag="ACHBBW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154437.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(3005..4345)
                     /gene="glnA"
                     /locus_tag="ACHBBW_002824"
     CDS             complement(3005..4345)
                     /gene="glnA"
                     /locus_tag="ACHBBW_002824"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9154438.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4373..4765)
                     /locus_tag="ACHBBW_002825"
     CDS             complement(4373..4765)
                     /locus_tag="ACHBBW_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9154439.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1480 a         1063 c          807 g         1626 t
ORIGIN      
        1 ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc
       61 aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac
      121 aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt gctacttttc
      181 tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg ctccgacaaa
      241 tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt ggaattggca
      301 tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt tatctggtga
      361 gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt tattgaactc
      421 atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt cagccgtctt
      481 cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac ccaaattctc
      541 gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg gattcgtttc
      601 agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac aagccatctg
      661 cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac gaatcgcttc
      721 gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc catctgcgtc
      781 tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc aatcgatctc
      841 gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg agatatatgc
      901 atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct tcaagttcaa
      961 gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat ctagttttaa
     1021 atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga tggagtaaag
     1081 tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa attgtgccaa
     1141 tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa tattcggttg
     1201 gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt tcatttctcc
     1261 agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat ctgtaccgac
     1321 atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat cccctactgt
     1381 tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc gcatgatttt
     1441 ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca agtattcgcc
     1501 cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt tggagtattt
     1561 gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg tttcagcacc
     1621 atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg gatccacgtt
     1681 gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca ataagcgtcc
     1741 taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg tcacaaaaat
     1801 atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa gtctcagaca
     1861 ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg taaacttctg
     1921 accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa gtcaagtact
     1981 caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag aaattatttc
     2041 ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga tataaacata
     2101 gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa gtgaaataat
     2161 agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa agaactactt
     2221 tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt ttctttcacc
     2281 cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt tggcatatct
     2341 tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa gatgaccttt
     2401 ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa
     2461 taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt tctcctgcca
     2521 actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt gaaattattt
     2581 cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta ttcaaaagcc
     2641 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
     2701 ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg tgttcaaaag
     2761 cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg
     2821 ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa acggtgttca
     2881 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
     2941 tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc acggtattcc
     3001 tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt tgtctgaatg
     3061 ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt
     3121 caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt gaaggtaagt
     3181 cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta cggtcaacag
     3241 ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac aatactgcca
     3301 ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac
     3361 cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat gcgacataaa
     3421 caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga ttgcaaacgg
     3481 ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct gtttcactta
     3541 gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac aaagacatat
     3601 tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa
     3661 gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata ttatcacaag
     3721 catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct acttcatgat
     3781 gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca cggcggcaat
     3841 tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc aaggtcgttg
     3901 tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt cctaaattga
     3961 aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat
     4021 cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga gcaacttttc
     4081 catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac aaatacatat
     4141 cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc attttatttt
     4201 ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta cctaagatgt
     4261 ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt ttgatttctt
     4321 cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt attcagtcaa
     4381 agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac ccaccttgcg
     4441 ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt
     4501 ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca gccatattca
     4561 atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc agcgaataca
     4621 tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca taatagcgaa
     4681 tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat acggacatag
     4741 atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag ttggctaaag
     4801 tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac atgagatctc
     4861 tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt tgattttcgt
     4921 accagatacc ttttattttt tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DAYYKC010000145         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000145 DAYYKC010000000
VERSION     DAYYKC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.149"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHBBW_002826"
     CDS             85..972
                     /locus_tag="ACHBBW_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9154440.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBBW_002827"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBBW_002827"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9154441.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHBBW_002828"
     CDS             2274..2720
                     /locus_tag="ACHBBW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9154442.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHBBW_002829"
     CDS             2993..4345
                     /locus_tag="ACHBBW_002829"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9154443.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHBBW_002830"
     CDS             4516..4803
                     /locus_tag="ACHBBW_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9154444.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYKC010000146         4911 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000146 DAYYKC010000000
VERSION     DAYYKC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.150"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1854)
                     /locus_tag="ACHBBW_002831"
     CDS             complement(88..1854)
                     /locus_tag="ACHBBW_002831"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154445.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1814..3568)
                     /locus_tag="ACHBBW_002832"
     CDS             complement(1814..3568)
                     /locus_tag="ACHBBW_002832"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154446.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3678..4247)
                     /locus_tag="ACHBBW_002833"
     CDS             complement(3678..4247)
                     /locus_tag="ACHBBW_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9154447.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4265..>4911)
                     /locus_tag="ACHBBW_002834"
     CDS             complement(4265..>4911)
                     /locus_tag="ACHBBW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9154448.1"
                     /translation="LKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMK
                     IIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKM
                     ELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDL
                     KEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1636 a          927 c          665 g         1683 t
ORIGIN      
        1 ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa
       61 ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa
      121 tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa
      181 ctcattatga gtaccttgat caactagttt tccttgatcc atcactacaa ttttatttac
      241 cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg
      301 atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc
      361 taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc
      421 tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt
      481 ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc
      541 ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg
      601 aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc
      661 atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt tcaagatact
      721 agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa
      781 agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc
      841 aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc
      901 tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga
      961 atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt
     1021 cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta aactaacata
     1081 tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat
     1141 cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa
     1201 gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt tctctctcaa
     1261 agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac
     1321 aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag
     1381 catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact
     1441 catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt
     1501 atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt gcactcttaa
     1561 atgcgcactt tctttataag tactattata taagttttca tactccctat ctaacaacca
     1621 atacatgaaa agcaaagtaa caaacgatac ggcaaaataa agccaacggg gctttgtatt
     1681 ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca
     1741 gtatacaaaa aaactagaaa tagaagcttt agttaaagct tctgcgccct gatcacttaa
     1801 agcaaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat
     1861 aattagtgac caattgttta tacacttgat gtttagccat taattcatta tgatttccac
     1921 gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata
     1981 atcgatgagc tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta
     2041 gttcatactc attatctgga tcaatagatg cactggcttc atccataata acaatctttg
     2101 catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa
     2161 ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc
     2221 aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag
     2281 aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg
     2341 tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc
     2401 ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg
     2461 attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa
     2521 cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct
     2581 gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg
     2641 tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag
     2701 aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg
     2761 gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa
     2821 aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa
     2881 acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa
     2941 atatctttat aacttgaatt ccgcggatat attctaccgt ttcagagctt aacacatcta
     3001 acgattcttg atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata
     3061 caccaattac taacatggcg attagaacca atccaatttt aaagttaatg aaaaagccta
     3121 aggcaactga taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa
     3181 ttgccatatg tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat
     3241 ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat
     3301 tagtctctaa gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag
     3361 ctcctaaagt taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc
     3421 ctacaataat tagagactga agaaatttgt aaatactgta atatccatag actgttaata
     3481 cgacagaaaa atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat
     3541 aactaaataa ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt
     3601 attcgtttac aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat
     3661 aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca
     3721 ccaatcatag ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca
     3781 tagtggatca ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga
     3841 aaacgatctg taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt
     3901 agagcccaag acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata
     3961 tcagctaaga tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt
     4021 aacgtaaaaa ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag
     4081 ttatagctaa tataaacaaa tatgattcca gataataatc caaaaatccc gggggatatg
     4141 ctgtgaccaa acgccccacc aatactacta accattattg aaaaaaagta aattactagg
     4201 gaaataactg tagctaaacc aataaaaata taatcttttg ttttcatata tattctcctc
     4261 aattctattt cgttatgtca ttaaaaatct gttctatcaa agttgaatct tctactctaa
     4321 gttttatatc tttttttatt ccaccatttt ttaaataaat aattctattg catgttttat
     4381 aaagaaattc cggatcatga gtagctataa ctactgcacc cttttctttt aagtctttaa
     4441 taaggtcagc aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata
     4501 gaaataaatt agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac
     4561 cggataaatc aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt
     4621 tttcttgctg atctttacta actatttcat tccccagggc taactcgctc ctcactgatt
     4681 ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt
     4741 tattttcgat tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg
     4801 ttgttttccc cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa
     4861 aatttatgtc ttttagaata ttttcataag taatttcttc aacttttaat a
//
LOCUS       DAYYKC010000147         4817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000147 DAYYKC010000000
VERSION     DAYYKC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.151"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHBBW_002835"
     CDS             complement(207..1169)
                     /locus_tag="ACHBBW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9154449.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHBBW_002836"
     CDS             complement(1171..2610)
                     /locus_tag="ACHBBW_002836"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9154450.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHBBW_002837"
     CDS             2795..4741
                     /locus_tag="ACHBBW_002837"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9154451.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1539 a          822 c          793 g         1663 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataa
//
LOCUS       DAYYKC010000148         4810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000148 DAYYKC010000000
VERSION     DAYYKC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.152"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHBBW_002838"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHBBW_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9154452.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHBBW_002839"
     CDS             complement(2066..3064)
                     /locus_tag="ACHBBW_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9154453.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHBBW_002840"
     CDS             complement(3290..3943)
                     /locus_tag="ACHBBW_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9154454.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHBBW_002841"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHBBW_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9154455.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1416 a         1007 c          746 g         1641 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag
//
LOCUS       DAYYKC010000149         4625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000149 DAYYKC010000000
VERSION     DAYYKC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.153"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHBBW_002842"
     CDS             complement(220..972)
                     /locus_tag="ACHBBW_002842"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9154456.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHBBW_002843"
     CDS             1085..2005
                     /locus_tag="ACHBBW_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9154457.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHBBW_002844"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHBBW_002844"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9154458.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4625
                     /locus_tag="ACHBBW_002845"
     CDS             3311..>4625
                     /locus_tag="ACHBBW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154459.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIES"
BASE COUNT         1620 a          748 c          900 g         1357 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtc
//
LOCUS       DAYYKC010000150         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000150 DAYYKC010000000
VERSION     DAYYKC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.154"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHBBW_002846"
     CDS             186..2855
                     /locus_tag="ACHBBW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9154460.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHBBW_002847"
     CDS             complement(2946..3560)
                     /locus_tag="ACHBBW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154461.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHBBW_002848"
     CDS             3680..4219
                     /locus_tag="ACHBBW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154462.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHBBW_002849"
     CDS             4216..4554
                     /locus_tag="ACHBBW_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM9154463.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYKC010000151         4580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000151 DAYYKC010000000
VERSION     DAYYKC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.155"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHBBW_002850"
     CDS             complement(12..482)
                     /locus_tag="ACHBBW_002850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9154464.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHBBW_002851"
     CDS             complement(641..1099)
                     /locus_tag="ACHBBW_002851"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9154465.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHBBW_002852"
     CDS             complement(1270..2415)
                     /locus_tag="ACHBBW_002852"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9154466.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHBBW_002853"
     CDS             complement(2428..3345)
                     /locus_tag="ACHBBW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9154467.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHBBW_002854"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHBBW_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9154468.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1429 a          840 c          867 g         1444 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt
//
LOCUS       DAYYKC010000152         4548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000152 DAYYKC010000000
VERSION     DAYYKC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.156"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(507..848)
                     /locus_tag="ACHBBW_002855"
     CDS             complement(507..848)
                     /locus_tag="ACHBBW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9154469.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(838..1119)
                     /locus_tag="ACHBBW_002856"
     CDS             complement(838..1119)
                     /locus_tag="ACHBBW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9154470.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1490..1729)
                     /locus_tag="ACHBBW_002857"
     CDS             complement(1490..1729)
                     /locus_tag="ACHBBW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154471.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1749..1997)
                     /locus_tag="ACHBBW_002858"
     CDS             complement(1749..1997)
                     /locus_tag="ACHBBW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154472.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2012..2578)
                     /locus_tag="ACHBBW_002859"
     CDS             complement(2012..2578)
                     /locus_tag="ACHBBW_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154473.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2579..3124)
                     /locus_tag="ACHBBW_002860"
     CDS             complement(2579..3124)
                     /locus_tag="ACHBBW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154474.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3150..3917)
                     /locus_tag="ACHBBW_002861"
     CDS             complement(3150..3917)
                     /locus_tag="ACHBBW_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFM9154475.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1289 a          954 c          596 g         1709 t
ORIGIN      
        1 cgctacgcga ccgttcgttc gtcttttcct ccgtgggact cacccaccgc aagcggttgg
       61 ttgagttgtg gctcctattc aaaattcact aatctgttat tttttatttc tattcaaata
      121 ggtaacgttc gtttgcaagc aaactctctc tatgtaactt gccagttaca tcagcggtaa
      181 cgctacgtcg caagcgactt gctctaatga aactgccgtt tcattctgcc tgttcgttcg
      241 tagcaagcta cttcactcac cgtatccgtc tgaattattg ttatttattg ctattcaata
      301 tccgtcagac attttattta tttacataca ttttatatag aatatatatt ataattcgta
      361 tattactaaa tatcattatt taacaattct taacgtttta ttaatgttta taaccacgta
      421 tatatttacg tatattttca tatacataaa tacgtttata taaacgtata tcttaatata
      481 catataaaca aaaaagaaaa gccttgttag acttttcttt gtaatttctc gataaaccta
      541 tttagctttt tcgtatcact agctgataac ataccaacct ttttgaatga tatgttctct
      601 cggcttttgg gaatagcctt taaagaccct acgtcaatcc atgattgctt tttaagtcct
      661 gctttttccc attctgcaat aggaaaatac accctcttta tttggtctga ttttttctca
      721 tattgacttg tgattccaaa gaattctact ctatcttctt tatcactaac caccaaaata
      781 ggtcttcttt taccaccatt tttatctgta aatgcaacat ataccgttac tatctcatta
      841 ggcttcatcg aaccactctt ctaacttttc atctgtatct agaacatcta ctggcatgtc
      901 cttagttagc tgttgcaatc tgattccttg cttctcctct tcacttagct ttatctctac
      961 tggaaactca cctctagcga ctacttgttt gtaaagaatt gttattgctg ttgtaggatt
     1021 taaacctagc tcttccaata tcatttctgc gttctctttt aaattcttgt ctattccgac
     1081 ttggattctg cttttttcag ttggattttt gaccttcatt tcatccactc cttttgagta
     1141 taaaacactc attatttatc aaattgtcca catttatgat tatattatac tcttaataaa
     1201 ctacattgca agtaatctaa tcaaattaag atgctatatc atacggtaat tgaacaaatt
     1261 gtcgctaacc cacacatacg gaatcgtaat gaatgaaaaa aagacaagcc tattcgcagt
     1321 aggcttgtct ttgtgcttct cttgtacaaa atcttcacat ttgtaaatta attttagcac
     1381 ctttgttagg aaagaacaac ttaaatgaga tgctaaagaa ttaatagttc tcaatctagc
     1441 atattcactt aataccatct tatcttcatt catggttcac cacctcatct taattatttt
     1501 caaattggca aatcaattta ttttgggtta acaaaactaa tagctgttca ctatcaaaaa
     1561 gaacctcaca aaattctttt tctatcctct cttctggcat gtaataacct atttgtttca
     1621 atatcttatc tggataacct tttcgttggg tttttctcaa taaatcttca tcatttccaa
     1681 cataataata agaacaccat tgcataccct tacttttaaa ttttgtcatg tttctatctt
     1741 cctttcactt attgacccaa ccatgaaaaa atatattttg ctaaatcttc cttttctatt
     1801 ccatataaaa cattctctaa atccaattta tattcctcgt caacaaggct ttctgtatcg
     1861 tcatcataaa gaacatatac tgcataattt ctttcgtaaa aggcattagc aagacttctg
     1921 ctaataccta ccattccttt ccatgtataa ccaaaatcaa ccaaatcttc atgatctaga
     1981 aaatccattc cttccatgct gattcacact cctattctgc tgttgtgtaa tatgcaacgc
     2041 aatcatctgc ccctactaac cacacacacc aatcttcaat ttcatcatcg cttctcttac
     2101 tcaattcctc tttggtacaa aaaactactt caccattttc tttttccatc tcataatctt
     2161 gtagcatgta ttcaaaccaa cttctttcgg ttggaaattc tatctcgtgg caactctggc
     2221 aaataaaatc accatattca ttgattcggt agcaaccttc atcttctaca aattcatgat
     2281 tacaacaaac acatctagta cctgttagga cttccccatt aatcgttgtt ctaacggtat
     2341 cttcccttgc tacgtgttca ctgctgacaa gccaatcaat gattttcttt gccaattctt
     2401 catcagaatc ggcaaagcct ttaaacgtgt ttctctttcc ttgaaagtag tatttgcaac
     2461 ttgcggtagt ttctatggtt ccataacttt tttggaataa ttcaatcatt tcaccatcaa
     2521 tcactttttg acttttcgct tctaaattag ggaactggtc ttttcttttt tcaaacactt
     2581 atttcacgct ctcttcttct aggtttattc cataattttc tagcaataat ctgtatccct
     2641 taataaaatc tttcgtagca aactcttttc cgctttcaca aaacttgttt agtcctatct
     2701 gttgaccctc aaaatcaaac aatcgcaatc tggttttatc atctagacta aatccatttt
     2761 catcgaacac catctctaaa tgagcatagc catcattgtt atctgaaaac ttcatcacat
     2821 cttcaatcgt ttcttgataa ggttcatagc gcccaccaaa ccctgtttca tcataagcac
     2881 tcactccaga ttcataggta acaaaattga taactcctgt acttctatca aagattcctt
     2941 ttgctaattc ttctcttgat ttatctcgta agtcgtatgc tttattgatt aaataaattc
     3001 cttgattcaa tacgtccatc agcatatatc gaccataccc ccactgataa tgaatcgaca
     3061 cgcttgcttt cgttttgctg ttaccatatt tgactgtaat taacgcttgt gttctttcgc
     3121 ccatcttgtt ttcctctttt ctctaatttt tataaagaac cattttcttg aaatgagtaa
     3181 tagcttgtgt ttttacttgt tacgatgata tggtctaaca attcaatacc aattgttttt
     3241 cctgcgtttt tcaaattcgt agtaaaactt ttgtcatttt cacttggaaa ttcatatccg
     3301 ctcggatgat tatgtgatac tgcgattttt gctgaattgt ttagcaaagc tagttgaaaa
     3361 atctctcttg gtatcgctac tgattgatta attccacctt tgaaaacttc gctataagca
     3421 atgacctcat tctttgtatt cagacacatc actagcaaag attcactagc attgtttcca
     3481 attaagttag tagcaaagtt tgctagacca accgagcttg tgactctata aaaacttcta
     3541 tcttcactga tatatttttg ttcgattttc aagacattct taatcaatcc ttcaccttca
     3601 ttctcggtct tgttttcaac ttcaatagat tcaagactgc tatttcccat atcagaaccc
     3661 aacaaagcat ttctgaaatc caataccttt tcaaagttat cagtcataac catttcatag
     3721 gtgctattcc attcattcaa atatttctct ttgatgagaa tgaacgctct cggtttactt
     3781 ctgccaattc tgatattttg tgtatcgtga aaaatatagt tatcaagtaa agtaccatcg
     3841 tacttttcat agttcccact gatatttgtt aacttgttta ccatttcttg attaacttct
     3901 aaaattctat ctctcatgtt gaacgcttct ttctgttttt gatttttggt ttttcaaaat
     3961 tcttttttgt ttggattgtt ttagagggag gtggtttttt cttcatcgct atttggtttt
     4021 gcattttatg tttcttcctt tcgttcattt atttatcttt ttcgcctgtc ttgccttgtc
     4081 gggagtgatt agaatgcgtt cttatctgcc ggattaatac aagtgtttaa gtaaattatt
     4141 ttcgtaacgc ttgcgtttag aaaaaaattt acgccacttg tattaatcca aagatgagat
     4201 aagctctaaa cacttctgac aaggtatgac aggcgaaaaa gataatgggc gtaaggaatt
     4261 ataaatggca aaaccaaata aagagcgatg aatttcaatg ctttcaggcg tagcttgaaa
     4321 gcctaaaagt cttgaagaat gaaagacttt ttaccttcaa agcagaccta gtaatcttaa
     4381 ataggcgatg gctatttaag attacggtgt tgacccacta aataaacaga aatggaaccg
     4441 atcaagcaaa atcggactag gcgatggcta ggacggtttg cggtggcttt aggaaacgtt
     4501 catggtgaga tgagcatatc tagcaatgtc gggctaagct atgcttag
//
LOCUS       DAYYKC010000153         4369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000153 DAYYKC010000000
VERSION     DAYYKC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.157"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..874
                     /locus_tag="ACHBBW_002862"
     CDS             173..874
                     /locus_tag="ACHBBW_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9154476.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1446..2462)
                     /gene="tsaD"
                     /locus_tag="ACHBBW_002863"
     CDS             complement(1446..2462)
                     /gene="tsaD"
                     /locus_tag="ACHBBW_002863"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9154477.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2459..2923)
                     /gene="rimI"
                     /locus_tag="ACHBBW_002864"
     CDS             complement(2459..2923)
                     /gene="rimI"
                     /locus_tag="ACHBBW_002864"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9154478.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2930..3472)
                     /gene="rimI"
                     /locus_tag="ACHBBW_002865"
     CDS             complement(2930..3472)
                     /gene="rimI"
                     /locus_tag="ACHBBW_002865"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9154479.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3456..4178)
                     /gene="tsaB"
                     /locus_tag="ACHBBW_002866"
     CDS             complement(3456..4178)
                     /gene="tsaB"
                     /locus_tag="ACHBBW_002866"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9154480.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1300 a          926 c          744 g         1399 t
ORIGIN      
        1 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
       61 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
      121 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
      181 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      241 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      301 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      361 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      421 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      481 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      541 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      601 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      661 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      721 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      781 aatcaaacaa ctacgtgatt ccggactgtt agattatgac aaaaagcgtt ttactttctg
      841 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
      901 gatatttatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
      961 ttagatcact gggcaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     1021 tgtttgcaaa aaatactgat agattaacta gaggaagcgt cttgtcctca tccgacctag
     1081 attattcgat attcctcgac aataaacgat taatcagtga gggcaatcag aggaacggat
     1141 aatgatgaat aagctgtaaa aaatagggct ttgacaaaag cgtgtcacag cccttatttc
     1201 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     1261 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     1321 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     1381 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcaga tttttcacaa
     1441 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     1501 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     1561 cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat
     1621 ccttcacgca acccttgatt ggccgctaca cctccagcta caatcagttg cttgactgga
     1681 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     1741 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     1801 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     1861 gctcgtggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     1921 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     1981 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     2041 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     2101 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     2161 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     2221 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     2281 cataaggtga tttgttctac gtgatggcgg ctagctactt caggaactac gccaccaaaa
     2341 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     2401 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     2461 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     2521 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     2581 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     2641 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     2701 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     2761 ggttatcttc aactaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     2821 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     2881 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     2941 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     3001 tcgttgttgc ttgaaaacct aacttccgat aaagtctttg tgcatcttga ttactcatac
     3061 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     3121 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     3181 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     3241 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     3301 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     3361 tagtcaattc tcttagtacg taacgacgat cgatttgttc gattgttttt tctgtataaa
     3421 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     3481 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctacccgttt taaatagcga
     3541 ggaagaaaag cttgtttctc tgcaactggt tcagctaaag cacctaattg agccacagtt
     3601 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     3661 tttatttcat agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     3721 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacaatttct
     3781 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     3841 atgggcacaa taattttatc cacaccgatg caattagccg cgatggtttt cagactagat
     3901 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     3961 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     4021 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     4081 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     4141 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     4201 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     4261 aaactagtac aatgatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     4321 acaaaaaagc tgcggtatca cttcatttcc ccacagcttt tccatcttt
//
LOCUS       DAYYKC010000154         4342 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000154 DAYYKC010000000
VERSION     DAYYKC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.158"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..954)
                     /locus_tag="ACHBBW_002867"
     CDS             complement(58..954)
                     /locus_tag="ACHBBW_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9154481.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(947..1627)
                     /locus_tag="ACHBBW_002868"
     CDS             complement(947..1627)
                     /locus_tag="ACHBBW_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9154482.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1628..2410)
                     /locus_tag="ACHBBW_002869"
     CDS             complement(1628..2410)
                     /locus_tag="ACHBBW_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9154483.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2403..3320)
                     /locus_tag="ACHBBW_002870"
     CDS             complement(2403..3320)
                     /locus_tag="ACHBBW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9154484.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3484..3738)
                     /locus_tag="ACHBBW_002871"
     CDS             complement(3484..3738)
                     /locus_tag="ACHBBW_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154485.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1276 a          946 c          756 g         1364 t
ORIGIN      
        1 tattaaatat atataagata atacattttt ctactagttc tcactaatct tgtataatta
       61 aaccctccgc aacttgacgg agatggagaa gatgcccccg tctaatctag cggtcatctc
      121 tcctcccatt ccttccacca atttttttga caggtaaagc ccgattccgc tagattccgt
      181 atttgtgcgg ctggtatttt cggtataaaa acgctggaag actttttcaa cagaggtttg
      241 cgttcctggt ttgataggat tggccactgt taatcgaatg aatggatcct cttttgaaaa
      301 tgtcaccttt gcttgtccat ctgcataacg caataaattc tgtatgatat tttgcagcac
      361 tcgcagcaac atgagcggat ctgctaatac agttgtattt atcgagtgtt cagcgaacgc
      421 tacctcgata tttttctcag aaaattgttc ataaaaagag aaaaatactt cttccacctc
      481 tttagaaaaa gaaagcgtat ggagatccat ttctttttgc ttcgtctcta ttcgagcaag
      541 atcataaaac tgatccgtca tactgagtaa tcttgcaaca gattgtttga tgatcgttaa
      601 atacttctcc cgtttctccg tatcctctac ctgttccagt aattgtacat aaccgttaat
      661 agcagtcaaa ggagttctga gatcatgaga aaggccgacg atcgatagtc tgatgttttc
      721 ttccatttct tgaaatgctt gtcctttttg ttcattttca tccaaccatg tattcagtgc
      781 atctactagg tccattagat tcttttctcg aaattcgact gaaagacgac caccatattc
      841 ttgtcgttca gaaagagtgg cgattttctt agttaatctc ttgatttccc tttgcaggaa
      901 gaaaataaaa cttcccataa agataacaac gagaagcaac agccatttaa tcaaatctaa
      961 accccactcc ccacaccgta tgaatataca cagcatgtgc ctttgctagc ttccctcgaa
     1021 gattggaaat atgcatattc actgttttct cactatcaaa atatggttct tgccaaactg
     1081 tttcataaag atttgctcgc gaaaatactt ttttcggatg ttctaaaaaa agcttcagca
     1141 gatcgaattc tcttcctgtc aaatggacag ccacaccatt cacatttaca tccctcgact
     1201 ctttgtccag taagatttcc ttgtaataga tgcgttgcga cagatctttt acttggacag
     1261 aagtctgatg acgaagttgg atgccgattc gtaacaaaag ttcttcgata tcaaatggtt
     1321 tcgtcacata gtcatccgct cctgctgcca acaaggaaac tttatctctt tgatcgcttt
     1381 ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg gatttctgct aacaatgctt
     1441 cacctgaaag ccctggaagc atgagatcta gaaggatcaa atcgacagga tatgtgctta
     1501 acaataattt tccttccgta cctgaataag catcaagaag tttgtatctc tgttcaagaa
     1561 cctcctccag taaggaatga acagcttcat cgtcttcgat aatcaaaata gtttccagca
     1621 ggaccaccta cttaatgtca gatttttgga aaataaacat tcctagagct gatgatacca
     1681 cgattgttac tagactgacc agaatcattt tcggtgcttg cgaataagtt gcttgttgcg
     1741 caaagtccgt atacatgagc cacgtttccg taaaccactc cgttagaaat tccaagcctt
     1801 tgatttttgc gaaaacaccg ggaatgaagg ccgttcccat gatataaaga aaagaaagaa
     1861 gcaaactgac actggtactt ctagtgatga atgtcaaagc cactggaaca gataatgtcc
     1921 ctaatgtcag cagaagcaat acaggaaata cttttagaac ataatttcca tagtttttga
     1981 ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc aagcatggct agtcccgtga
     2041 tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag aagtttcgac acataataag
     2101 tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt accgttttga aattcttccc
     2161 caatataaaa gcacgcaaat gctgcgaaag ccagaagaat ccatgcagcc attttttcta
     2221 ccattaaaat ccctgttgca ccgttgatga ctgtatccgt tgcttggttt aattcattgc
     2281 cgtattttgt atgcatatac cattcgttcc aagaataaaa aagcggaagt acgaccatca
     2341 gtcctagtga gacccacatt ttacggctct tccatacttt tattttttca gcagttaata
     2401 gattaaacat gtggattgcc tccagttaat tgtaagaaat aatcttccag tttttgccca
     2461 actaggtgaa taccatccac aggaactttt gcttctacca atgcttgatt gatggctgct
     2521 acttggtcaa ttccttcaaa aagctggata acacggctag attgtacaaa ataatcccta
     2581 taaccaagag agtcgagaac cgttactgct tgttccgtcg ccgaagtgtc aatttttata
     2641 aattgacgag attcttgtgc caattcttct ttcgataatt ctttgatcaa gtgtccttca
     2701 tgcaaaatac catagtgagt ggcgatctgt gacaattcat ctagcaagtg gctagaaagc
     2761 aagacggtga tgccccgttc ttttaccaaa cgttggatga tttctcgcat ttctgccatc
     2821 cctgctggat ccatcccatt cgtcggttca tccaaaatca aaaattctgg attcgtgatc
     2881 agtgcatttg caatagctaa acgctggcgc atacctaaag agaaattctt tgctttcttt
     2941 ttccctgtat tttccaatcg catcattttc agcaggtctt cgatttctct atccgacaca
     3001 ccgccgttcg ctgcttggat acgtaggtta tctcttgcag ttaattctgg ataaagagca
     3061 ggggtttcaa tcgattgacc catacgtcgt cttgctgcct gcaaatccgt cgttccaaat
     3121 aattcgatcg aaccttcatc aatcgagatc aaacccataa tagttctcat aaaggtagac
     3181 ttacccgctc cattttcccc aatcaatcca tagatcattc cacgtttgat ttcgattgat
     3241 actttatcta atgctttggc agcaccgtat tttttggtta taccagttgc tttcaagacc
     3301 gtttcatttc caaaatccat acttatcctt ctttcttatt gatgagtgaa gtatagttga
     3361 cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg taaattttcc aattgaatgt
     3421 caaaaaaaac aatcggacaa tacgtgtctt tcctattatc aggcagtttg ttatacgctt
     3481 gtatcagttt cctgattctt tcttttcgac caaagtcgtt tctctgtatt cctcttcact
     3541 gaaaattttt gcttctttca gatcaagctt gagtaatttt accagaccgg ttataaggat
     3601 aactatagca agactccctc ccagaaaaat tagactgtct cgcagacgtg gttgcgttgt
     3661 cttccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca ccatggtaat
     3721 cgttgttatc gttgacataa gtcgtccttg tatatgcgtt ggaacttctc gctgaagcca
     3781 gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg aaaaataata
     3841 catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa agacttttca
     3901 caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     3961 atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga agctgaccac
     4021 ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt atctttttcc
     4081 cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta acatagctaa
     4141 aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag gattaaggta
     4201 actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact cgaacgataa
     4261 tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac ctggtttttt
     4321 cttcgtatca ttgaataagt tt
//
LOCUS       DAYYKC010000155         4127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000155 DAYYKC010000000
VERSION     DAYYKC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.159"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(681..754)
                     /locus_tag="ACHBBW_002872"
     tRNA            complement(681..754)
                     /locus_tag="ACHBBW_002872"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Ile,seq:cat)
     gene            complement(943..1962)
                     /locus_tag="ACHBBW_002873"
     CDS             complement(943..1962)
                     /locus_tag="ACHBBW_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9154486.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1959..2183)
                     /locus_tag="ACHBBW_002874"
     CDS             complement(1959..2183)
                     /locus_tag="ACHBBW_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9154487.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2180..2473)
                     /locus_tag="ACHBBW_002875"
     CDS             complement(2180..2473)
                     /locus_tag="ACHBBW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154488.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(2510..2647)
                     /locus_tag="ACHBBW_002876"
     CDS             complement(2510..2647)
                     /locus_tag="ACHBBW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9154489.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(2649..3080)
                     /locus_tag="ACHBBW_002877"
     CDS             complement(2649..3080)
                     /locus_tag="ACHBBW_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154490.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(3094..3588)
                     /locus_tag="ACHBBW_002878"
     CDS             complement(3094..3588)
                     /locus_tag="ACHBBW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154491.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(3588..4037)
                     /locus_tag="ACHBBW_002879"
     CDS             complement(3588..4037)
                     /locus_tag="ACHBBW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154492.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT         1169 a          837 c          668 g         1453 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attataagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggcagt ggtttttggg gaacgcttta aactcttctt tattattatc tcacaatata
      481 acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac agaaatataa
      541 ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc actcatttga
      601 gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct aacacagaac
      661 gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
      721 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
      781 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      841 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
      901 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
      961 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1021 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
     1081 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg ctctataaat
     1141 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
     1201 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
     1261 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
     1321 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
     1381 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
     1441 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
     1501 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
     1561 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
     1621 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
     1681 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1741 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1801 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1861 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     1921 cgttaacagg ctcatcgcag taatagtaat tttctttttc atttgtttcc tccttcttcg
     1981 ctttcagccg agaacatttt gtaggttcga tttgatacac ccaacacact ccctaaaaac
     2041 gcgccaaaac cagtaatgat gacaacacag atatctgtgt actgccaatt gagcgcttta
     2101 ccaactaacc ccacgaaagt agctagtgcg ggaataatta ccagtgcgaa ccattttagt
     2161 acttcgaacg ttttattatt cattttcttc tctccctaaa caaagttttg atttgttgcg
     2221 tgtgttccac caatttttct gtatgtgtat ctaatctttc atcgtgtttc tttagttctt
     2281 catgaatcat caatcgatct gatttgctcg attctaaatc tttagtcagc aaatctaaat
     2341 tgtggcttac ttttgaaaga gtctcagtaa ttttcgagaa agatgcagca atcggtctaa
     2401 ttactaataa aatcaaagaa acgatagcgg ttattgatcc tgctatcatt ccccattccc
     2461 ctaaattaat catgtgacaa ctcctttacc ttaaataaaa acgcatcaat taagatgcgc
     2521 tcttatcttt attaatgatt ttatctgctt cttcgtctgt aatgcataat ggaacgaaaa
     2581 ccattacttg ttcgttagtg aaacagcccc agtcatacat tagtttgata tcttcatatg
     2641 aatacatgtt acttcccacc ttgtaattgt tttttgattt cttcgatttc cttggtattt
     2701 tgtacagaag caagcatcgt ttttgaattc aactgcgcta aactttctga cttttctttc
     2761 aataagacat tttcttgctt gattgctaca ttgtttagca ttgctttaga atttagctgt
     2821 ttcaattcat cattttctgc cttcagcgtt tcgtataatg ccttaatatt agccaattca
     2881 tctacgctat caacagcatt cccttttttc tcttcttcag tagctaactc aacccactgt
     2941 tttttatcaa aatcaaatct tggtctccag ttaggaactg gtggtttaat ctctgtacag
     3001 ttttcaggga tattttcttg attgttcaaa atgatttgct cgaatccgta aggcttgatt
     3061 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     3121 ataagctgat ccataacttg aatttcttct ccatttaatg gctccgtctg cgccaataga
     3181 tagttgagca ctgttcaaag tagaacggtc tattgaccca accagttgtt caaaactaat
     3241 tggtgtccga tagccttctg gaacagttaa aataaccgaa tcattcccgc cactactttt
     3301 tccgtttaaa gccacaaaat atatagaaac tgtttttcct tcacgataaa gctttgctgt
     3361 ccctgtatta ccgttagtga caaccagatt taccacttca tatctgttat catcagtcgt
     3421 caaaacacgt ttattattga ttgttacaga acctgaaaaa ttcttatgtc ctgcaatggc
     3481 ttggtctcca tctgtctgca ccaatgcttc tttaccatca atagcttcta catgcgtttt
     3541 taagtattta gcaactccgt cttcttttaa ttgaacaata tcagccatta aaccgtcccc
     3601 actttctcaa atgtaatatt cgctaatcca tcaagtttca ctttatcggt tgctgacatt
     3661 aaacctgctg tcgtagttgt agcattacct ggatttttct gtgctccagc tgcaattcca
     3721 tccaacttag ttttatctgt ggacgacatc aacccatttg ctgtagttgt agctacagct
     3781 gtagttgtgg catttattcc aggatcacct ttatctccct ttggtaaaac aaaattaaat
     3841 ctagctgcag atgatgttcc tacattcgta acagaagcgg ttgaaccact agaaacggtt
     3901 cctatggtaa ttgttgctgc ttggccagga tcgcctttat ctcccttcac cgttgttggt
     3961 ttgccttcta tagcattcca atgagtttgt ggataaacct gtacattgct ttgttttatt
     4021 tttacgatat ctgtcattct ctatacctcc ccaatttttt caaaagtaaa atttggaatt
     4081 ctttcgtttg tgtaattttc tgcttgattt acagcttctt ggaattt
//
LOCUS       DAYYKC010000156         4091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000156 DAYYKC010000000
VERSION     DAYYKC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.160"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1239
                     /locus_tag="ACHBBW_002880"
     CDS             46..1239
                     /locus_tag="ACHBBW_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9154493.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1232..2959
                     /locus_tag="ACHBBW_002881"
     CDS             1232..2959
                     /locus_tag="ACHBBW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9154494.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3353..3550
                     /locus_tag="ACHBBW_002882"
     CDS             3353..3550
                     /locus_tag="ACHBBW_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9154495.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            3552..3863
                     /locus_tag="ACHBBW_002883"
     CDS             3552..3863
                     /locus_tag="ACHBBW_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9154496.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            3967..>4091
                     /locus_tag="ACHBBW_002884"
     CDS             3967..>4091
                     /locus_tag="ACHBBW_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM9154497.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT         1434 a          633 c          882 g         1142 t
ORIGIN      
        1 tttttcagaa agaaaaatga atttacataa aaaaggagaa tttgtttggt aaaagtagaa
       61 gtaaaacatt taacaaagat atttggtaaa aagacacaag ccgctttaga tatgatgaat
      121 gatcaccagc cgaaaacgga gattttaaag aaaaccggtg caacggttgg tgtgtatgac
      181 gtgaattttg acgtaaaaga aggagaaatc ttcgtgatca tggggctttc cgggagtggg
      241 aaatccacat tgatccgtct attgaatcgt ctgattgagc caacttcggg cagtatctat
      301 attgatggag aagatgtatc gaagttaagc aaagaagaat tgcgagaagt ccgtcgtcat
      361 aaaatcaata tggttttcca aaacttcggt ctgtttccac atcgaacgat cttggaaaat
      421 acagaatacg ggttagaagt ccgtggtgta cctaaagaag aacgacaaga aaaagcagaa
      481 aaagcattag aaaattctag tttgctttca tttaaagacc aatatcctag tcagttatct
      541 ggtgggatgc aacaacgggt tggcctagcc agagctttag caaatgatcc tgagatcttg
      601 ctgatggacg aagcgttctc ggctcttgat ccgctgatcc ggcgagaaat gcaagacgaa
      661 ttactggacc tgcaagccaa tgttcaaaaa acgattattt tcattaccca tgatttgaat
      721 gaagctttac ggattgggga cagaatcgcc ttgatgaaag acggcgaaat catgcagatc
      781 ggaacaggtg aagaaatatt gaccaatcca gcaaatgatt atgtacgtga atttgtggaa
      841 gaagtcgatc gttccaaagt gcttacggct caaaacatca tggtacctgc attgacgacg
      901 aatatcgaat ctgatggacc aaacgttgca ttgacaagga tgcgtaatga agaagtcagc
      961 atgctgatgg ctgttgacag aaagagacac ctgaaaggga tcatcactgc agatcaagcg
     1021 ttagaagcta ggaagcaaaa acgtccattg atcgacttcc tagatgaaaa tgtgacggtg
     1081 attgggaaag acatggtggt aagtgatatt ttcaatatta tttatgactc accgactcca
     1141 cttgcagttg tagaggatgg aaaattaaaa ggtgtcgtca tccgaggtag tgtgattgaa
     1201 gcattagcag aaacaagcga ggtgagtgaa catgaataat ctattgaact atcaattacc
     1261 agtggcaaac tgggtagaaa atatcacaga atggtttacg acaacttttt ctggcctatt
     1321 ttcattttta caaacgattg gacaggcagt gatgtcgggg attacaaatt tactgttagt
     1381 gattccagct cctttgttta tcttgttatt gacggtagca gctttcttta tttctaagaa
     1441 acgtccagga cttacgttgt tcacattaat cggtctatgg tttatttata accaaggtct
     1501 atggaatgat ttgatgaata ctgtcacact tgttctttta tctagtgtga tctcgatcat
     1561 cattggggta ccattaggga tcttgatggc aaaaagcagt aaagcccaaa gtattatcaa
     1621 accgatcctt gactttatgc agacaatgcc aggattcgtt tatttgattc cagcagttgc
     1681 atttttcggt atcgggatgg ttccaggggt atttgcctca gtcatctttg ctctgccacc
     1741 aaccgttcgt tttacgaatt tagggattcg acaagttcct aaagaattag tagaagcttc
     1801 tgattcattc gggagcacaa gtcgtcaaaa actattcaaa ctagaattgc ctttagctaa
     1861 aagtacgatc atggcaggga tcaatcaaac aacaatgctg tcactttcaa tggtcgttat
     1921 tgcctctatg atcggtgcac ccggcttagg acgcggcgta ttatcagcat tgcaaagagc
     1981 acaagtcggg aatggatttg tcaacggggt tgcattagtt atcttagcaa tcatcattga
     2041 ccgttttaca cagcatttga accagccaaa caataaaaaa gctgcaggag caacaacaca
     2101 gaaaagtaaa aaacgtcaag gactgatcat tggtgctgta gtagtagtga tcttaggtgc
     2161 gattggtata gggagttttt catctgccaa agaaacaaaa cgtatcaact tatcttatgt
     2221 tgaatgggat acagaagttg cttcaacaaa tgtagtcggt gaagtattga aacaaatggg
     2281 ctatgatgta acgatgacgc cattagataa ttcaatcatg tggaaatctg tttcaaatgg
     2341 cgagtcagat gccatggttt cagcttggct acctaaaact catggttcac aatatgcaca
     2401 atataaagat caagtcgaag acctaggtgc caatttgaca ggagcaaaag ttggtttagc
     2461 agtgccagcc tacatggatg taaattcgat cgatgagtta accgatcagg caggtaagaa
     2521 aatcatagga atcgagccag gagctggtgt agttacggct gctgaaaaca cgatccaaaa
     2581 atatgacaac ttgaaagact ggaaagtaga gacatcttct tcaggagcaa tgaccgttgc
     2641 actaggtcaa gcgatcaaaa aacacgaacc aattgtagtt acaggatgga cacctcattg
     2701 gatgtttgcc aaatatgatc tgaaatactt agaagatcct gaaaacggaa tgggaagcga
     2761 agagcagatc catacgatgg ttcgaaaagg attgaaagaa gaccagccag aagcttacaa
     2821 agtattagac aatttccatt ggtcagaaaa agatatggaa aaagttatgt tggaaatcaa
     2881 caatggaaaa gatccacagc aagcagcaaa agactggatc aaagagaacc aagaattagt
     2941 cgaaagctgg aaaaaataaa aagacagaca cgaaataaat ggagcttcca aaattggatc
     3001 aaatatccaa atttggaagc tccatgttcg tctctatctt ttttgtctat aaaaaaagag
     3061 aatgcgtagc tcatcttcga aacgttcaaa gacgccgaca aactattatg aatttatggg
     3121 tgcgagtcaa agagatcgat cgtaggtatg aaagactaat gtgattaaag tttattatgt
     3181 acgaagtgca ttctcaaaaa atattaaaga atatacatag tttatatact taaaagtgat
     3241 taaattctta ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt
     3301 aatgaatgtt agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca
     3361 tttaaaaatt ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg
     3421 aaaatattat ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa
     3481 ggcaactact tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg
     3541 gaagtgctaa aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat
     3601 ctataagtaa agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa
     3661 aattagaaaa acaaaaatat gaattagatc ccagtccttt aataaataga ttggtgaatt
     3721 atctatattt tactgcttat actaataaaa taagattcac tgaatatcaa gaggaattaa
     3781 taagaaattt gagtgaaatt ggaagaactg ctggaataaa tggtttatat cgagcagatt
     3841 atggagataa atctcaattt taatgatact tataatatta aatatgtctc aaaatttttt
     3901 gattagaaaa ataggtatat tgaatcagtg acataaaagt atttatagtt tgtaggggga
     3961 atcgtaatgg aagaaaaaaa tagactaaat gctaaacaat gttcagatca agagcttaaa
     4021 aaaattaaag gaggtgctgg aacaaaacct caaggaaaac cggcatcaaa tttagtggaa
     4081 tgtgtttttt c
//
LOCUS       DAYYKC010000157         4072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000157 DAYYKC010000000
VERSION     DAYYKC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.161"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..607)
                     /locus_tag="ACHBBW_002885"
     CDS             complement(65..607)
                     /locus_tag="ACHBBW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9154498.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            742..1131
                     /locus_tag="ACHBBW_002886"
     CDS             742..1131
                     /locus_tag="ACHBBW_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154499.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1135..1899
                     /locus_tag="ACHBBW_002887"
     CDS             1135..1899
                     /locus_tag="ACHBBW_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9154500.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2028..2975
                     /locus_tag="ACHBBW_002888"
     CDS             2028..2975
                     /locus_tag="ACHBBW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9154501.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3105..3845
                     /locus_tag="ACHBBW_002889"
     CDS             3105..3845
                     /locus_tag="ACHBBW_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154502.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1358 a          687 c          818 g         1209 t
ORIGIN      
        1 taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg
       61 tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt
      121 ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt
      181 cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca
      241 ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat
      301 cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt
      361 cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat
      421 tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct
      481 cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta
      541 acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat
      601 caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg
      661 aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata
      721 aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg
      781 ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta
      841 gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt
      901 ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt
      961 tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc
     1021 gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt
     1081 ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat
     1141 ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa
     1201 ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag
     1261 ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat
     1321 gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg
     1381 gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga
     1441 ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc
     1501 gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca
     1561 cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca
     1621 aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa
     1681 aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat
     1741 atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt
     1801 tttcgttcct caaaaacaca gcgacatatg gtacaaatac aactaatgga agaagcttcc
     1861 acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat
     1921 gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt
     1981 ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag
     2041 cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc
     2101 cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt
     2161 accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat
     2221 ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg
     2281 ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg
     2341 tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg
     2401 accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag
     2461 acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc
     2521 aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg
     2581 gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc
     2641 attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt
     2701 gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg
     2761 gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa
     2821 tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg
     2881 gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt
     2941 ttcctgaact agttatcaga gagacaacga attaagtctt attactatat atatatttat
     3001 gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg
     3061 tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac
     3121 gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca
     3181 agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt
     3241 ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc
     3301 gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag
     3361 gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa
     3421 aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc
     3481 atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta
     3541 aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt
     3601 taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc
     3661 cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt
     3721 tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac
     3781 gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta
     3841 aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc
     3901 cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc
     3961 gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg
     4021 cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga at
//
LOCUS       DAYYKC010000158         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000158 DAYYKC010000000
VERSION     DAYYKC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.162"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHBBW_002890"
     CDS             complement(<1..1157)
                     /locus_tag="ACHBBW_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9154503.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHBBW_002891"
     CDS             complement(1160..3409)
                     /locus_tag="ACHBBW_002891"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9154504.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHBBW_002892"
     CDS             complement(3506..4009)
                     /locus_tag="ACHBBW_002892"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154505.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAYYKC010000159         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000159 DAYYKC010000000
VERSION     DAYYKC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.163"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHBBW_002893"
     CDS             85..1434
                     /locus_tag="ACHBBW_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9154506.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHBBW_002894"
     CDS             complement(1562..2152)
                     /locus_tag="ACHBBW_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9154507.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBBW_002895"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBBW_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9154508.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYKC010000160         4015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000160 DAYYKC010000000
VERSION     DAYYKC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.164"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..1633)
                     /locus_tag="ACHBBW_002896"
     CDS             complement(128..1633)
                     /locus_tag="ACHBBW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9154509.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1630..2316)
                     /locus_tag="ACHBBW_002897"
     CDS             complement(1630..2316)
                     /locus_tag="ACHBBW_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9154510.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2509..3930)
                     /gene="gndA"
                     /locus_tag="ACHBBW_002898"
     CDS             complement(2509..3930)
                     /gene="gndA"
                     /locus_tag="ACHBBW_002898"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9154511.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1102 a          852 c          691 g         1370 t
ORIGIN      
        1 taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat ccctgaattt
       61 ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa caagtaaagc
      121 acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa attgtccctt
      181 gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt tgtttagcga
      241 tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc actcgataaa
      301 aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg
      361 cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt cgtgtagttg
      421 aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga ttacgataaa
      481 tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag tctggatata
      541 atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt gtttcattcg
      601 cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga actaagcttt
      661 tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc tcaggatcat
      721 ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca ggtgtcctta
      781 actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa cgcatacggt
      841 caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg tcgatttcag
      901 gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct ctgagtacct
      961 tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt aagatcaaag
     1021 aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca tagaaagaag
     1081 ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta gaatatatag
     1141 gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga actttacgtg
     1201 tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc tcgtccaaat
     1261 cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggga tcgatcttca
     1321 ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca tttgtcgctt
     1381 tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta gccaaacgag
     1441 aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca atcagattga
     1501 tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac
     1561 tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt tccgattttt
     1621 tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga tatagctttc
     1681 ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca ctacgtttgt
     1741 ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg ctaataccac
     1801 gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta gttcaattac
     1861 ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc gataagtaat
     1921 agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca acaatgcacg
     1981 aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg
     2041 gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa tgattggtgt
     2101 atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg ggagcatcag
     2161 atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag ttcggccgtt
     2221 gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa atcgtgccaa
     2281 gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc tttctattct
     2341 actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc tatagtcatt
     2401 ttatcatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat cgacttcagc
     2461 tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt attcttcatg
     2521 gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa
     2581 gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt atgaatcgaa
     2641 gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag ccaaggcaac
     2701 gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat catctagcaa
     2761 taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca agaatcgagc
     2821 tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca agtcccaatc
     2881 aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca tgattttact
     2941 gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg tgaactcaaa
     3001 atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat
     3061 atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt caagcgcgct
     3121 ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca catctactac
     3181 tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga tttctatcaa
     3241 atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt cttctactga
     3301 aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca tatctccata
     3361 ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc caatatatgt
     3421 gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg gtgcaactag
     3481 cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag ctccttcttc
     3541 accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact cttcattacg
     3601 acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag
     3661 gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga ccattagcaa
     3721 gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg ttgctttgaa
     3781 atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac gattgaataa
     3841 agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca taacggccat
     3901 accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg ggatttctcc
     3961 ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac aacat
//
LOCUS       DAYYKC010000161         3937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000161 DAYYKC010000000
VERSION     DAYYKC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.165"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..466)
                     /locus_tag="ACHBBW_002899"
     CDS             complement(293..466)
                     /locus_tag="ACHBBW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154512.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(469..867)
                     /locus_tag="ACHBBW_002900"
     CDS             complement(469..867)
                     /locus_tag="ACHBBW_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154513.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1083..2138
                     /locus_tag="ACHBBW_002901"
     CDS             1083..2138
                     /locus_tag="ACHBBW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154514.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2158..2511
                     /locus_tag="ACHBBW_002902"
     CDS             2158..2511
                     /locus_tag="ACHBBW_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154515.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2693..2980)
                     /locus_tag="ACHBBW_002903"
     CDS             complement(2693..2980)
                     /locus_tag="ACHBBW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154516.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2993..3811)
                     /locus_tag="ACHBBW_002904"
     CDS             complement(2993..3811)
                     /locus_tag="ACHBBW_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154517.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT         1343 a          707 c          558 g         1329 t
ORIGIN      
        1 tgaactaata aatagcttaa cctcgctatt ttgttttttc tgatcactat ctatacagca
       61 ttagtaaata gaattttaaa aataaaaaaa ttaagacaaa aaagtatcaa cataaacata
      121 tacatataca cgtttatgtt gattcactta atttgtcctt taaaaagcac ttattttaat
      181 ataatgttat tttataatct tattacaagc tgattgttag gcgttgtcct ttcattacat
      241 ttattaacgg ttattttatt gtttaattta ttaattattt ttgatctaaa acttagctta
      301 acaaataaat aattaaccaa ccgccagata aaaatgcgat cattgctaat acataagcaa
      361 agataaaaat atgttcgatc agcttaaacc ctgatgcgat taagcagacc aagtaaaata
      421 gaaattctct tacagataat ggtaattttc taactagcat actcataatt aacaccccaa
      481 agtaatattg tattttgaac attttacatc caatacagca tcaatcaacc attgccaatc
      541 aaaaatacaa ggcttcattg aagtatgttt ttcagatgaa aagatataat ataatttctt
      601 cggaataata ttttcagttg ttgggatcaa gctaaatcta ataccgtcta actcatagct
      661 accagctttc tctccattaa aaatttcctt agcactttct tctaaagaat caaaatattt
      721 ttcgacagca atctttgaag cttttactat tgaagcattt cccttttcga taaaatctgc
      781 ttttgtttta ccgacaataa gtaattcttg atcagtaaaa tcttctaaaa taatggtagt
      841 gtaattctta tttcttctca atcgcatcag gatcactcct taatataatt tatgttttta
      901 taatatcata ataagataaa tttgcaagca tttatttaca cctttttatt tacactttat
      961 tataattaat tagactttta tgattgcatt tttttcttat aaacagttgc aactaatagt
     1021 aaataggtgt aaactatttt cgtaccgaaa taccataaac acaaacagaa agaggtcata
     1081 ccatgaaaga accattattg ttttccgttg acttagggaa taaatcagta aaagaaatga
     1141 gtacacacgt ttctccactc tcacttccct ctcgtatttt gaacaaaaat atcattaatt
     1201 caggacgttt ttctaatata ggttcctcat taaaacgatc actaaacgtt tctgaatata
     1261 gcctagtaaa cactactgaa agctattact ttgggaagga tatacacgag ctaggaaaag
     1321 atgactacgt gatagaatca ctaggttttg ggatcagcag atacaagcag gaaacctacc
     1381 aaaatatcct atctttctct ttagctaacc tagcacgttt agaagatcaa tctgatgacg
     1441 ttttaaccgc taatctagtt ttaggtctgc cttcatccga ctataccgaa acaatcgtca
     1501 aatacgtgat gcaacaagcc acacgacaac attcagttga agttgacggt aaagcttata
     1561 atataaacgt agataccgtt tgggtactgc cacagccgat tggaacacta tataacctcc
     1621 tacttgctga tgatggtagt gtacaagatg aaacattgat cagtcagaag atcggttttg
     1681 ttgatattgg cggtggcacg aaactttttg atgttgtcca aaattttgaa attgacgaaa
     1741 acaatcgtga acaaaaggat accggcattt ttactttata taatcgtatc gtaaatatgc
     1801 ttgaccttcc gatcagtcct aatgctttcc aacttgaaaa gattatccga gaaggcaaag
     1861 aaaaaggcgt atatctattt aagccaagcg gaaatgaaag ttttgatatt tcagaagtag
     1921 ttgaaagaga aataaaaaca tacaccaata tggttgttgc agaagtttta gctacattca
     1981 aaaacttcac aaccttagat aaagttgtct ttactggtgg tggagcttct attatcgaca
     2041 aacaacttgt aaaagataaa attccgcata ccttgtttgt tgaagacagt gaattttcta
     2101 atgtaaaagg cttttacaag tttggtttaa ctcaataaaa cagcgaagga gatcaacatg
     2161 aataagggaa aatcagagcg ttatgtagtt ataatcaata gagaaaaggt tagcgagtta
     2221 cctttagcag agtttcttga atccgaatat gaaacactag gcgttaaata ctctcagacg
     2281 atcaaaaaag ctttgaacct atatcggcgt gtaaagaaca atgaagttac aattattgaa
     2341 gcaggcaacg atagaaacga tagcaaagta gcagaagtaa agaataaacc aactgatcat
     2401 aaccctattc aacaagagca tccttcaacg gttacatccg atcattcagc aaagaaagaa
     2461 aagccaatgc tgaaagggtt caatgcacca agtaacaaag acttacttta aaaataaaaa
     2521 ggcgaggtta caatacctag cctttttatt atgtacaaat ataaacataa acatatacgt
     2581 atacatgtat ataaacatgt atacgtatat atccataaac acgtatatta aatttagtta
     2641 agctatacct aaccgcttat tccaataaaa aaagtgatca gctttaaact gatcactcac
     2701 caaaaacttc atcatacaag ttatctatca tttcaccaag taaagctgaa tctgatcgaa
     2761 ttcctaactt ttcgctgatt ttagatagtt tctttctttg cgatggttga agcgtgaatt
     2821 gataattttt cttcttttca gaactatcac tttttagttt agctaccaaa gaacttgttg
     2881 tttctttagg tgtttgttta ggttcttcaa ctgcttcaac cacttcagga acttcttcta
     2941 cttcagcttc agcgaatttt ttaaatctat tttctttcac ttcaatcact ccttattctt
     3001 gtgccgtatc aattattttg ttgtatagcg taaacgtgct ttcaaagaaa tccttattct
     3061 tacgatagat attcttatcc ttagcgaact cagcaatatt cttaccttct aagctagttg
     3121 cattaagcaa ttccttctct tgtacatatc caagataatt agataacgtt ggtaccagtt
     3181 ctaggaactc acgacttgaa ttagtattat gtttgatcct gctaccgatc gtagcgtatt
     3241 tagtttccat cagtgtttca ccagtcttaa tatcaatcaa ctgttttgct agtgcatcaa
     3301 tatctttacc aagcttcagc aaagcttcaa aaccatcttt agatggatca gaaatgccta
     3361 cgacaagatc agatacagcc caagcatttt gagctagtag acctgcatca ttatgagtat
     3421 caatcagaat tagatcatac ttctctttaa gctgttcata gttaacaacc atccatctaa
     3481 acagtagggt tctgttgtta ggcatatcac gcatttgcat ctcaacttgt tttaaatctt
     3541 cacctgctac taaaatatcc atgttctcat ttacagctaa aggttcagct gatccatgaa
     3601 gatataaatg attgattgta ttctctcttt tcgctttact gaaagcatca ggaaatcgtt
     3661 ttgataggtt ttgcgactta tcttcatcaa tatacagcac cttcatacct aaattttcga
     3721 cagcatatcc acctagattg tagattgttg ttgttttacc tgttccacct ttttcaatgt
     3781 ttccagtaat gattgacaac ttcttttcca tgattaaacc tcccttatta accttttgta
     3841 actccattat acactataaa cacgtttatg tatatgttta tatacataaa cgtgtttata
     3901 tttatatttg cttttatatt ctacaacata aacatat
//
LOCUS       DAYYKC010000162         3920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000162 DAYYKC010000000
VERSION     DAYYKC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.166"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1193
                     /locus_tag="ACHBBW_002905"
     CDS             <1..1193
                     /locus_tag="ACHBBW_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154518.1"
                     /translation="LKLTTITMTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLV
                     YKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRN
                     EKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTG
                     QRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASV
                     LQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNELIVNFADLCSGEC
                     LINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKR
                     ESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGI
                     EVRS"
     gene            2043..3368
                     /locus_tag="ACHBBW_002906"
     CDS             2043..3368
                     /locus_tag="ACHBBW_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9154519.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3361..3708
                     /locus_tag="ACHBBW_002907"
     CDS             3361..3708
                     /locus_tag="ACHBBW_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154520.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            3705..3887
                     /locus_tag="ACHBBW_002908"
     CDS             3705..3887
                     /locus_tag="ACHBBW_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154521.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         1532 a          501 c          752 g         1135 t
ORIGIN      
        1 tgctaaaatt gacaacaata actatgacaa aaaaaataga agtatatgtt gatggatcac
       61 aagaagaaag ggattccttt tttgattttt tttggaaaaa tagtagagaa tatgtaaaag
      121 cgttgaatct ggtatataaa cagctaatgt ttgaagagtt gataaaagat gcaattaaag
      181 aaatggatca aggttatgct gagagagaag aaagtattca aaatcggatt gaaaagttag
      241 catatactga tccaatagat caaaaaaaac ttgataaagc aaaagctgaa ttatctaaat
      301 tgagaaatga aaaaaataaa gaagcgagag aaatattaac gcaagcgata gggttgaaga
      361 gtcaaacgag aacacgagat gtagcaaaaa aattagactt tgactttgga gattttgttg
      421 attccgcaaa tatgaaagct agtaaagatt ttggcaatga ttttatcgga attgtgaccg
      481 gacaaagaac gcctagaata tataaaaata gaaacaaata tggattacta ccttttagag
      541 gtcgagatat taaaatccat aaagatgata agttttatat ttatttacct aaaggcttta
      601 ggtttaaagt gaatttaggt agcaagccaa gaaaagcaag agatgttgct tcagttttac
      661 aacacattat ggatggcgat tacaaattaa atcaaagtaa tcttattttt gatggaagaa
      721 aattattttt taatctaaca atttcttttg aaaaaagcta taatgaaatg gaattcaaga
      781 aaggtaatga attgatcgta aactttgcag atttgtgtag cggtgaatgt ttaattaatg
      841 ataaatctgt aatgtctttt ggtgatagcc aatatataag aaacttcaaa agtaaaattt
      901 tagcaatgaa atcaaaagaa caaagccgag gagtcttttc taagggcggt catggtcgaa
      961 aaagaaaatt agatcaagat cgatgggaag caataaagaa aagagaaagt aattttgtta
     1021 aaacttataa taatcaatgt tctgcaaaaa tcgttaaaac tgctattcgc aataaagctc
     1081 aaattattcg tatcattgaa ccagataagg cttatggcga atgggcttac tatcaattga
     1141 aagaacaact agtaaacaaa ggaaaaagat atgggataga agtcagaagt tagtaaatta
     1201 aacaatcgtt gatttggcaa cgaaatagtg agggaaaatc cactcactga agggtaaact
     1261 ccgtcaagtt gtgtgaccag acggaaggac gaatgttgtt gaggttttag aaccatataa
     1321 aacagcatgt ttctcaaatt tttaggataa gggaaattgc tagcagtttg ttttagaacc
     1381 atataaaaca gcatgtttct caaatgagta ctttaaatta attgatggcg ttcctgcttt
     1441 agaaccatat aaaacaacat ggatttaaaa caagaagtaa gtcagttctc cttttacttt
     1501 agtaaggaaa aataaagtat tctcaataaa acttaaaata agcaaacctt tgttttaata
     1561 gctaatcgaa gtaaaaccca aagccatatg aacccctgaa tcagcgaaag tttatgatga
     1621 tttggattac taatagttta ggaattagat ggtaaatcta aattaaacaa tcgttgattt
     1681 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttatgtg
     1741 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catagttctc
     1801 aaattgttta aaatgattgt caagttaaaa aaatctggga tcatataaaa tagtatagtt
     1861 ctcaaatatg attagaaacc ctattaaaga gcaatattaa gccaacttac ttaaatcgtt
     1921 taattaagaa caataatctc ttttagtcag acttttagtc tgactttttt gtatttccaa
     1981 aatttattct ttacaaaaac gaacatacgt ttgtataatt gcttgtgaat gggggaataa
     2041 aaatgtttga ttacagtaga gaaccggtga atgactattt tttaatcgac atgaagtctt
     2101 tctatgctag cgtggaatgt attgaacgaa atttagaccc aatgactaca ccgttaattg
     2161 tcatgagtag atcggacaac actggttcag gtctgactct agcttcttca cctactgcaa
     2221 agaaaaaata tcatattact aatgttagta gagctactga tttaccttat ccattaccaa
     2281 aagaacttca tattgttcca ccacgaatga acttatatat aaaaaaaaat atggaagtta
     2341 atcagatttt tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat
     2401 caattttaaa agtgacaaaa tccttaaatt tatttactag ttcaggaaca agaagcgaga
     2461 gaagaagaga attagctaaa ataatcaaag atgagattca gaaagaaaca ggtctaatag
     2521 caacagtcgg aataggagat aacccattat tagctaaact tgcactggac aatgaagcga
     2581 agagtagtaa agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga
     2641 aaataaacga gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc
     2701 atatgggaat tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc
     2761 agttaggtgt aatcggactc cagttatatt tccataccaa tggaattgat cgtacggata
     2821 ttgctattcc accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa
     2881 aagattatac gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag
     2941 ccattcgaat tagacaacac aattgcaaaa ctggttgtgt acgcttagca attggtacgt
     3001 caattttgga agaaaaatca ggtttctcgc accagatgaa aataccaatt acagataata
     3061 cgaaagaatt acaaaattat tgtctttttt tattcaataa gttttatgag gggcaggaag
     3121 tacgacatat aggagtaact tatagcagat tagtatacac agattcccta caattagatt
     3181 tatttagtga tccaagaaaa aagattgctg aagaaaattt ggataggatt attgataaga
     3241 ttagacaaaa atatggattt acagctatta tacatgcttc atctatgtta gaaggtgcaa
     3301 gaagtattac gagaagcact ttaataggag gacacgcagg aggattagga ggtttgcata
     3361 atgattgaat ataacgatag aggacgttta aaatggtcgg gattttattt gtctgatcat
     3421 actgaaagaa tcaatgcaga caatgtacaa cgtgctaatc ataatttggc aaaagaacaa
     3481 atgacgactg aagagattgc gaaaatactg agaactgctg tcataaaaaa tagacccgtc
     3541 tgcattcaaa aagaggaacg aaacgctgaa ggttactatc cagatgatgt agtaggaaaa
     3601 attattggat atgacgagct tggtgtattt gtggaagaag aaaaagttca ctatgatgaa
     3661 attaggaatg tttctttctc ggaattatta aaatggagtc agatatgaac attattaaaa
     3721 aatacgaatt cggttatatt acgtatgaac agctaataga ggaattatgg ggatatggtc
     3781 aacaattaat taatgaagtg gggattgatt gcttttgttt ctatattgaa tccggagcag
     3841 gataccataa gaacagatac tatatatttc catatccacc aaaatagtct cttagatatt
     3901 tttaaatttt ctccaaatac
//
LOCUS       DAYYKC010000163         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000163 DAYYKC010000000
VERSION     DAYYKC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.167"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHBBW_002909"
     CDS             complement(717..3866)
                     /locus_tag="ACHBBW_002909"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM9154522.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYKC010000164         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000164 DAYYKC010000000
VERSION     DAYYKC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.169"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHBBW_002910"
     CDS             complement(<1..353)
                     /locus_tag="ACHBBW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154523.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHBBW_002911"
     CDS             complement(613..1161)
                     /locus_tag="ACHBBW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154524.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHBBW_002912"
     CDS             complement(1162..2019)
                     /locus_tag="ACHBBW_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9154525.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHBBW_002913"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHBBW_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHBBW_002914"
     CDS             complement(2581..2910)
                     /locus_tag="ACHBBW_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9154526.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHBBW_002915"
     CDS             complement(2987..3259)
                     /locus_tag="ACHBBW_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9154527.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHBBW_002916"
     CDS             complement(3259..3522)
                     /locus_tag="ACHBBW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9154528.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYKC010000165         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000165 DAYYKC010000000
VERSION     DAYYKC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.170"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHBBW_002917"
     CDS             72..155
                     /locus_tag="ACHBBW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9154529.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHBBW_002918"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHBBW_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9154530.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHBBW_002919"
     CDS             complement(1280..2143)
                     /locus_tag="ACHBBW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9154531.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHBBW_002920"
     CDS             complement(2145..2417)
                     /locus_tag="ACHBBW_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9154532.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHBBW_002921"
     CDS             complement(2434..2643)
                     /locus_tag="ACHBBW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9154533.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHBBW_002922"
     CDS             complement(2721..3617)
                     /locus_tag="ACHBBW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154534.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYKC010000166         3695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000166 DAYYKC010000000
VERSION     DAYYKC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.171"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..506
                     /locus_tag="ACHBBW_002923"
     CDS             54..506
                     /locus_tag="ACHBBW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154535.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="ACHBBW_002924"
     CDS             520..1209
                     /locus_tag="ACHBBW_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154536.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="ACHBBW_002925"
     CDS             1334..3685
                     /locus_tag="ACHBBW_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9154537.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1258 a          479 c          771 g         1187 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gttta
//
LOCUS       DAYYKC010000167         3670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000167 DAYYKC010000000
VERSION     DAYYKC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.172"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1162
                     /locus_tag="ACHBBW_002926"
     CDS             <1..1162
                     /locus_tag="ACHBBW_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9154538.1"
                     /translation="KNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGTME
                     TIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSIKS
                     KLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDTTG
                     PSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGHNI
                     APGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKANAE
                     EIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRPLK
                     TWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            1208..2704
                     /locus_tag="ACHBBW_002927"
     CDS             1208..2704
                     /locus_tag="ACHBBW_002927"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9154539.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            2785..3468
                     /locus_tag="ACHBBW_002928"
     CDS             2785..3468
                     /locus_tag="ACHBBW_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9154540.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            3477..>3670
                     /locus_tag="ACHBBW_002929"
     CDS             3477..>3670
                     /locus_tag="ACHBBW_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154541.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         1254 a          641 c          785 g          990 t
ORIGIN      
        1 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
       61 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
      121 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
      181 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
      241 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
      301 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
      361 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
      421 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
      481 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
      541 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
      601 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
      661 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
      721 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
      781 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
      841 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
      901 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
      961 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     1021 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     1081 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     1141 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     1201 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     1261 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     1321 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     1381 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     1441 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     1501 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     1561 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     1621 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     1681 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     1741 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     1801 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     1861 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     1921 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     1981 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     2041 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     2101 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     2161 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     2221 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     2281 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     2341 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     2401 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     2461 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     2521 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     2581 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     2641 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     2701 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     2761 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     2821 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     2881 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     2941 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     3001 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     3061 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     3121 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     3181 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     3241 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     3301 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     3361 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     3421 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     3481 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     3541 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     3601 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     3661 accgtttatt
//
LOCUS       DAYYKC010000168         3537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000168 DAYYKC010000000
VERSION     DAYYKC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.174"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1036
                     /gene="galE"
                     /locus_tag="ACHBBW_002930"
     CDS             47..1036
                     /gene="galE"
                     /locus_tag="ACHBBW_002930"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9154542.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1048..2532
                     /gene="galT"
                     /locus_tag="ACHBBW_002931"
     CDS             1048..2532
                     /gene="galT"
                     /locus_tag="ACHBBW_002931"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9154543.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2556..3536
                     /locus_tag="ACHBBW_002932"
     CDS             2556..3536
                     /locus_tag="ACHBBW_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9154544.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1201 a          646 c          775 g          915 t
ORIGIN      
        1 tatataatca acaaacacaa ttttactaat caaaggagtg ttttttatga caattttagt
       61 actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc aaaaggggta
      121 tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac atccagatgc
      181 tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat ttgaaaagga
      241 accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat ctgtcgaaaa
      301 accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg aagtcatgca
      361 cgagttcaat gtgaataaaa tcgtgttctc ttctactgca gcaacttacg gagaaccaaa
      421 agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg gtgaaagtaa
      481 attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc gctatgtcgc
      541 tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag aagatcatac
      601 accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa gaaaagcatt
      661 ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag attatgttca
      721 agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag aaggaaatga
      781 aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa tgctagaagc
      841 agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa gagctggaga
      901 tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt ggaaacctga
      961 atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca gccatccaaa
     1021 tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg tgattttgcc
     1081 acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt acagaatcgt
     1141 cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc agttaaggaa
     1201 gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg tgttctttca
     1261 gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct cacaccgcca
     1321 ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga ggaagcaact
     1381 gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc catcgcaaaa
     1441 aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat caatttatct
     1501 aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa agtagactat
     1561 cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa ctatccagcc
     1621 cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg attccaatat
     1681 tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca ccgcccgatg
     1741 aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt gcctcattac
     1801 tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc tcatgatcat
     1861 tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa acttatcaaa
     1921 ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc tgtgatccgt
     1981 ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt ggaaaagtgg
     2041 aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg tacaccacac
     2101 catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga ccttgtcctt
     2161 cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca taaagatgtc
     2221 caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc tgttttacca
     2281 cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga agaaaaccag
     2341 atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc tgatttgacg
     2401 aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc acgtgtactg
     2461 gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga acgatttact
     2521 gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa gacattgcgc
     2581 agttggcagg agtttctcct gctactgttt caagagttct caactatgat cctgatcttt
     2641 ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta aattatacga
     2701 aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag tggtacgacg
     2761 atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc gaaaaaaagg
     2821 cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt gatcttccga
     2881 cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa atgaaaggat
     2941 cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc gactctctag
     3001 ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc cataaagcgc
     3061 aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag cggctaaaag
     3121 atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac gaaggtccaa
     3181 ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag tatttaaaaa
     3241 cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg attggcgcac
     3301 tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg atgggtttca
     3361 atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa gtttataccg
     3421 aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc aaagcaccgg
     3481 tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg aaataaa
//
LOCUS       DAYYKC010000169         3448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000169 DAYYKC010000000
VERSION     DAYYKC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.175"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..598
                     /locus_tag="ACHBBW_002933"
                     /pseudo
     CDS             278..598
                     /locus_tag="ACHBBW_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            776..1135
                     /locus_tag="ACHBBW_002934"
                     /pseudo
     CDS             776..1135
                     /locus_tag="ACHBBW_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1199..>3448
                     /locus_tag="ACHBBW_002935"
     CDS             1199..>3448
                     /locus_tag="ACHBBW_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9154545.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKF"
BASE COUNT         1265 a          461 c          640 g         1082 t
ORIGIN      
        1 aaagtcccta tattaacacc aacagtactt acaatgttgg agatggaaaa taatattaat
       61 tatattgact caggttcaga tttgctgatt aggtggcagg taataactaa ttttcttatg
      121 gaattaaaaa aatacagtaa tactcaaacg aagttctctt gaagttataa agaatacaat
      181 aaatataaat ttcctttaga ctatattaaa atcagcaatc gaaggaaaat aaaagaagaa
      241 aatggtacgc aaaatctaac tattagagca aaaacaggaa gctgctttgg aagatagtcc
      301 cgaattgttt ccgatgttaa aagatcgttt aaatgaattg actgaaaaac gaagacagtt
      361 acaaattcga gagaatgaaa tcttagggat ctttcaacaa caaggggaac cgattcaagt
      421 caaagatgtc caacagatat tgactagttt ggattgcttt cttatccaga gtgagaagaa
      481 gcaagttaaa gctttgtatc acacctttat tgagaaaatc acctttgatc ctaatcagaa
      541 gtagatattg ccgatcccga tgagattcac acctactgtg atttgataat tgaatgagta
      601 atatcaaata gccgtatcca aaccaaagga tatggctatt tttgtgttag ggatatacgt
      661 cacgtagctt gttatcaaga ataagtttct ttattttttt gctttttcta aaactgagta
      721 tgctaaaata agagggtata tagcaacttt aaataagaga taggagtgta tctttttggg
      781 tgcagaatta aatcagaagt tatttagtgc ggcagataat ctccgtagta aaatggatgc
      841 gtcagaatat aaaaattatt tattggggct gattttctat aaatatttat ccgaccgttt
      901 gttggaacaa gtggttcttt tagcagatga atccttagag gaatatgaca cagtaagcaa
      961 acaaacgatg ttgtatcgtg aattactttc agatgaagaa tcaaaagaag atctgattgc
     1021 aacaatcgtt gatattttgg ggtatgcgat ttcgccagag tatcttttca acgtcttagc
     1081 ggatcaagca aaacaagcaa cgttccaatt gaatgatcta aacaaagcct ttttagcaag
     1141 tcatatttat ggaaaaatac tgtcggcaaa attcgaatag tgtcgaggag gtgctgtgat
     1201 ggaagaatta aaagaacgca ttgtcaatta tgtcatagag aactctgaga ttaaaaataa
     1261 atgggggaac aatacagcac agaaaatatc tgaaaaatta aatgttagtc gtaatattgt
     1321 ctctcaatat ttgaatgaat tcgtaaaaga tggagaattt ggaaaaatca atactcgtcc
     1381 agtaatcttc ttttctttac cggagaaagt tccaaatcaa caaattttta gctcttcaga
     1441 ggaagcggaa aaatggtatg atagctcttt tagggttgga atagaaaggg ttatcggaag
     1501 tcaaggaagt ctgcgaaatg taattgctca aattaaagca gcaatctact atccgccaaa
     1561 tggcttaccc attttacttc atggcgagac tggaactgga aaaagctatt tagcaaatta
     1621 tgtgtataag gatttgttgg ccaataatta tatagaagag aaggcatctt tcatttcagt
     1681 taattgtagt gaatatgcca ataatccaga acttttcttg tccaattttt ttgggcatgt
     1741 aaaaggtgca tatacaggag ccgaagatgg aagaaagggt ttagcagaat tagctaataa
     1801 tggagtctta tttttagatg aaattcatgc attgagcaat gaatgtcagg agaaactttt
     1861 tcagtttatg gatacgagga aatttcatcg gatgggtgat aatgaaaact ggtacacatc
     1921 aagttgctgg attatatttg caacatcaga accacctgag gataaactat tatcaacttt
     1981 gttgagaaga atacctgtaa agctaacact tccaacatta gacagtcgtt ctctttatga
     2041 aagaaaacag ttaatcaaga tattttttaa atcagaagaa gaaagactag caaagaaaat
     2101 actgatatct caacaattat tagatttttt attaaactat cgctttagtg aaaatgttgg
     2161 caatttgaaa aatatcattc aattgacctg tgccaatgcc ttttatttct tagatgattt
     2221 ggaagaactt tatattcaga tgtatcattt accacaatgg gtgaatttca acggcatgaa
     2281 ggttatgaaa agacagaatg aattaaaata tattcaaatg gatgatttgg aaaaagtaga
     2341 cgaagttttt aatatcaacg aacttataga gtatctttat caaacgtgga agaaagacac
     2401 tgataagcag cagctttttg agaaatatga tagatatttt agacaaaaga atgttgatat
     2461 ccaaaataat tcagctttaa catatcccac ctatcaagag attcgattta gacagtatgt
     2521 taaggaaata gagagagatt ttcaaataga atttgactat tattatcttc aaataacgtc
     2581 tatctttttg aattcttgga tttatcaaat ccaattaaca tcggacgata aattatattc
     2641 cttcatcaat gatattaata gttggtgtag tcgagaagtt caattagtca atcgttttat
     2701 tcaaataggg gatatttatt taacaaaaat tcaaaaaaat gttttggtta ttttatttaa
     2761 gttaatgtgt agtaaagaat ttcagcaaaa acgtttagca ctgattttgg ctcatggaga
     2821 tctaacggca acctgtatag caagtactgc taataggatc gttgagagat atatatacga
     2881 cgccattgat atgccactaa tgacttcaag tagtgagatt gcagacaaaa ttaatagata
     2941 cttacaagag tgtaccggat atgaagaaat tattttactt gttgatatgg gttcactgga
     3001 gaaaatttat ctaaatttgg aactagacgc tcaaaaaaaa ttcggactac taaacaatgt
     3061 ttcaacaaga atggctattg atgttgcaaa tcagatatta ttagatttac cgcttaaaca
     3121 gattcttgaa caagtaaaag aaaatcaaaa tgtggattat atttatgaat catcaaatat
     3181 taagaaggat atgattgtct gttcttgtgc aagcggatta ggaacttcgt ttaaattaaa
     3241 aaggatactt gaagatagct ttccaaggga tactgatctt gtcatagaaa cgtgtgacta
     3301 ttactcttta gtgaatgaaa actatttatc ggagcttcgt aaagagaaca atattctttt
     3361 tattgttgga acactaaatc cacatctaga agatgtagaa ttcttttcaa ttgaagatat
     3421 gattttagga atgaatataa gaaaattc
//
LOCUS       DAYYKC010000170         3411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000170 DAYYKC010000000
VERSION     DAYYKC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.176"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACHBBW_002936"
     CDS             complement(<1..2303)
                     /locus_tag="ACHBBW_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9154546.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACHBBW_002937"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACHBBW_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          934 a          765 c          550 g         1162 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat a
//
LOCUS       DAYYKC010000171         3388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000171 DAYYKC010000000
VERSION     DAYYKC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.177"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHBBW_002938"
     CDS             complement(63..2555)
                     /locus_tag="ACHBBW_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9154547.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHBBW_002939"
     CDS             complement(2562..3029)
                     /locus_tag="ACHBBW_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9154548.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="ACHBBW_002940"
     tRNA            complement(3165..3238)
                     /locus_tag="ACHBBW_002940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="ACHBBW_002941"
     tRNA            complement(3296..3369)
                     /locus_tag="ACHBBW_002941"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DAYYKC010000172         3277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000172 DAYYKC010000000
VERSION     DAYYKC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.178"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..614
                     /locus_tag="ACHBBW_002942"
     CDS             <1..614
                     /locus_tag="ACHBBW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154549.1"
                     /translation="KKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIE
                     EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKN
                     ILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALS
                     EDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            601..2022
                     /locus_tag="ACHBBW_002943"
     CDS             601..2022
                     /locus_tag="ACHBBW_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9154550.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            2035..3042
                     /locus_tag="ACHBBW_002944"
     CDS             2035..3042
                     /locus_tag="ACHBBW_002944"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9154551.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3054..>3277
                     /locus_tag="ACHBBW_002945"
     CDS             3054..>3277
                     /locus_tag="ACHBBW_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM9154552.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKK"
BASE COUNT         1178 a          413 c          545 g         1141 t
ORIGIN      
        1 aaaaaaaaat tgacgattat tttgtctatt gtccggaatt gttactttat gatgtaaaaa
       61 atataaaaaa aattcccaaa atagatgaaa acagtaaatt gatgaaaaac atacaatcaa
      121 tctttgggat agaagaagtt tttgtcggag ataaatgtat cttttttgat caacctttat
      181 tatctgatgg ttatggcata gatgaaacag atattttcgc taaagtaaat agtatatttt
      241 ctggaaaaaa agaaacttta attaagcttc atcctagagc aaggacaaat cgttatggtg
      301 aaaaaaacat attgaattcc agtttaccgt ttgaagtctt gctacttgaa aatgacttct
      361 cttcaacact tttgattagt cctatatcta caatttcttt ttcaccatct atgatgtttg
      421 aaaagacgat caactctatt atgttggcta aattaatttt aaatgaatat acagcattat
      481 ctgaagataa aaaagaaaca ttgacaaata ttgttctttt ctgtgaacat tataaccgtc
      541 tatcagaaga aaaaatttta ctcccagaaa actgggcaga attaagaaag ttggtagagt
      601 atgggattat ttaacacact ttccaagtca aaattagtta agtccagttt ctggtatact
      661 atagggaatt tttttattaa gggaatttca ttcataacta ttcctatttt tgtaaatatt
      721 actactcaag cagaatatgg tttgattaat aatttcactt catttgtatc aattttttcg
      781 attcttgttg ggctaagcct aaattcggca attaataatg caagttttga ctataaagat
      841 gacataaatg aatttatgtc atcggtactt tctctagcta cactttcgtt aattgtgttc
      901 ttattaatag ggaacatata ttttttgttt tcaaatgctt tttttgatat gaaccaactt
      961 gtatttaatt tgatgttatt acaaagtttt gctagttttg taattagtta tatccagtct
     1021 tattttacga ttaactttaa ccattttaaa tatttgattt tatcttttat atctactttt
     1081 ttgaatattg caatctctgt agctttgatg cagacattat attttgatga taaatatgtt
     1141 ggcagagtag tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga
     1201 ataatgataa agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc
     1261 tctttgccgc taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt
     1321 atgattaatt cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata
     1381 ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat
     1441 ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta
     1501 gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg
     1561 gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt aggctatttc
     1621 tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt
     1681 attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata
     1741 cctaggttag gaataattgc tgctggatac acaaccgtat ttacgtattt tttacttttt
     1801 atttttcatt ataatatcgc taaaaaaatt acgaataaac aattatttga cacaaaaaaa
     1861 atatgtatca taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat
     1921 caaatgttga ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt
     1981 ttaaagatta agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt
     2041 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
     2101 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
     2161 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
     2221 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
     2281 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
     2341 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
     2401 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
     2461 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
     2521 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
     2581 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
     2641 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
     2701 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
     2761 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
     2821 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
     2881 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
     2941 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
     3001 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     3061 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     3121 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     3181 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     3241 aagaggacag ttctcaattt gaaatgctaa aaaaatt
//
LOCUS       DAYYKC010000173         3113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000173 DAYYKC010000000
VERSION     DAYYKC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.179"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            787..>3113
                     /gene="esp"
                     /locus_tag="ACHBBW_002946"
     CDS             787..>3113
                     /gene="esp"
                     /locus_tag="ACHBBW_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9154553.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1137 a          461 c          603 g          912 t
ORIGIN      
        1 tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa aaattggatc
       61 agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt tcagaaaagt
      121 taaacaacta caagttaata gattataagt atttgagtga aagcaattaa ttattcatgt
      181 agagtactag acactttaaa tatggacaat tcaattataa agaaaaagaa tcaataattt
      241 aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa acgagtgaaa aaatgttgat
      301 aactccaatc attcgtttaa gattctagga atcattcaga aatgctgtga aaccgatact
      361 ttataattat aaagaaggtg atttcaaatt ccgaagaagg aatttgaagt caccttttta
      421 cttatgtcac agtctcattc agtaatgcgg gataaggctc cttattccgt tatttgaata
      481 ctctgaagtt taatctaaga aagggtaata ttttgaaaac atgcttcatc gcatagaaat
      541 gaacttaatt tcagaaagaa tacatacata tctttttata tgttttcagc tgctgaatgg
      601 ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt
      661 catgaaattc tctaagtaaa ttagttaaca taaggttagt aagattccta ttctgtgaat
      721 aatacgtatc agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt
      781 tgaataatgg ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta
      841 ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt
      901 cttgtcttgg gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca
      961 cttgaaacag cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt
     1021 caggagaatc ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa
     1081 gaactaactg gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat
     1141 aatattgcag catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct
     1201 aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc
     1261 tttgtaagaa ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt
     1321 cgtgcaagtg atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat
     1381 ggggtaatta cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat
     1441 ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa
     1501 attgtaccgg gtattgatag tacgggctac tttttaacag attcagatga tttctataat
     1561 agcttagatg tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa
     1621 tatgagaaac ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct
     1681 ataatcttag atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag
     1741 ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt
     1801 atacagatgc acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga
     1861 acaaaagtag ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt
     1921 tcaataacta ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca
     1981 gtcgttattc gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg
     2041 aaagcaacag cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct
     2101 tggttgtcgg atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat
     2161 ttgcaagata ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat
     2221 ccatatatcg ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg
     2281 aaagtattca tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa
     2341 ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca
     2401 gatactgtag aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa
     2461 ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt
     2521 aaggaatcac caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct
     2581 aaagaatcaa ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca
     2641 gagcctgata cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga
     2701 aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaaaat
     2761 gatgcaacat acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct
     2821 acatttgata aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat
     2881 aataagtact tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca
     2941 gctaaaggtg taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac
     3001 caagatggtt ctgtaggcac gaccgattta gcagtaactg taagtaaaga tatttatgaa
     3061 aatccaggag aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAYYKC010000174         2814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000174 DAYYKC010000000
VERSION     DAYYKC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.180"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..511
                     /locus_tag="ACHBBW_002947"
     CDS             188..511
                     /locus_tag="ACHBBW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154554.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="ACHBBW_002948"
     CDS             675..929
                     /locus_tag="ACHBBW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154555.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="ACHBBW_002949"
     CDS             999..1244
                     /locus_tag="ACHBBW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9154556.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="ACHBBW_002950"
     CDS             1320..1685
                     /locus_tag="ACHBBW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9154557.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="ACHBBW_002951"
     CDS             complement(1746..2309)
                     /locus_tag="ACHBBW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154558.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2580..2702)
                     /locus_tag="ACHBBW_002952"
     CDS             complement(2580..2702)
                     /locus_tag="ACHBBW_002952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154559.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT          833 a          493 c          431 g         1057 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caat
//
LOCUS       DAYYKC010000175         2752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000175 DAYYKC010000000
VERSION     DAYYKC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.181"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(368..907)
                     /locus_tag="ACHBBW_002953"
     CDS             complement(368..907)
                     /locus_tag="ACHBBW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154560.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(1047..1727)
                     /locus_tag="ACHBBW_002954"
                     /pseudo
     CDS             complement(1047..1727)
                     /locus_tag="ACHBBW_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(2049..2270)
                     /locus_tag="ACHBBW_002955"
     CDS             complement(2049..2270)
                     /locus_tag="ACHBBW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154561.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2368..2613)
                     /locus_tag="ACHBBW_002956"
     CDS             complement(2368..2613)
                     /locus_tag="ACHBBW_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154562.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          846 a          513 c          385 g         1008 t
ORIGIN      
        1 aattttcaaa tacgcaaaat taagtacgaa agagttgaaa caaatttgga gaaaaatgag
       61 catagaatgt ctgatgagtt aaatagacct aaaaattatc taaaagtgga gcaaaattag
      121 gaattacaaa caaaacacta aattagttta tataattttc cgtaatattt aatatatatg
      181 aaaaggaaaa cgtagaaaat tacgtctacg ttttcctatt tagttctata ttttcattaa
      241 attttatatt tgctcttttc acttttcgga ataaattcac tgtgactctt gataccttct
      301 tttatatatt gctaatgtat tttctccatt gctatgaaaa tagaatcaac atatttataa
      361 tatctatcta atctattttc atttcaccgc agcatcttat taaataacgt gtaaataaga
      421 atataaaagt tattgaacta attaataaag atagaatcat cgtagttgga tacgtagatg
      481 aaacctcaac tatagttctt ttccccgtac ctttaataga cactaaacca ttgtcatttt
      541 ctatagtgct ctttttccca tttactttca caactgttcc ccaataattg aatacaggta
      601 atgtgatatt ttgctcatcg gcatcttcta ctgtatagac tattttggaa ttatatgctt
      661 tatgagatgc attctcccac cgatcaccaa cttttacttc atgtttcttt aaatgatctt
      721 tatatttcaa tgcatctaat ggtgcatagt catataggcc tccagttctt gaggatgtat
      781 aacgtgtaac atcttttttg gtcataacta aacgaccttt atacgactga attttatgcg
      841 aataatttgc ttctgttgct gcagtcacag taaaaataat aagaactatt cctacaaggc
      901 tccacatact ttttttgttg cttgttttcc actttaaaaa cactatggtt aagattaatg
      961 aaccaaaaag cacttgaaaa cccaaggttc tcggttctgt tgcgaagttt gaaaatcaaa
     1021 cgcgccctct ctgaactcca aatatcttag gctgttattc ccattaatac cttgatttca
     1081 gtagacaccg aaaagccgaa gagcgttcca tttcttcggt tctttttata tattcctcga
     1141 atggtctcca tgcccttaat cgtggaagag gctgtacgga gactttgata aaatttattt
     1201 cgtcgtttaa taggtcgatg gtcttgttct attaaattgt taagatactt cacagttcgg
     1261 tgctctgtct tagtatataa acccacactc tgtaactttc taaaggcgga gccaagagaa
     1321 ggtgctttat cggtcacaat tgctttcggc tcaccaaact gtttatggag tcgttttaag
     1381 aaagcatagg ctgcttgcgt atcccgtttc tttcgtaacc agatatctaa ggttaagccg
     1441 tccgcatcaa ttgcacgata aagataatgc caacgtccct taattttgat ataggtttcg
     1501 tccattttcc atgaatagaa ggattgtcta tttttcttct tccaaagata atagaggact
     1561 ttgctgtact cttataccca acgataaatc gtagtatgac aaacatttat tccacgatca
     1621 tataacaatt cctgaacttc acgatagctt agattgtaac gcaggtagta accaacagcg
     1681 acaataatga cgtctttttt gaattgtttg cctttaaaat gattcattac tctgtcctct
     1741 ctgtcttttt tctcaatttt acactaaaat agattttttg gaaaactttg caacagaacc
     1801 gtttttacta tatacttctt cccagaactt gtccgtctcc ttcaaattat ctctacctct
     1861 cgagttcatt gggtggtttg cccatttatt aaacatggaa tcacctttct catactcata
     1921 tatttatgtc ctatttcaat tatatttata tatgcttaaa taaatttgta tcctgaaatt
     1981 tattgtaata tgaagctgta ttttacatat agtaaccaaa gagttaataa aataaaaaga
     2041 gcgaaccatt atagatcagc tcttttttca ttgctgtttt taatcttatc tagaatcact
     2101 tcaaatgttt caaaggtatt tacatctaac tgacataacc aaagcaagta cttcttttct
     2161 ttgtaagtcc aatcaatatt acggtaagag gacattagat tatggtacct tgctctttgt
     2221 tcaactgaag ccaattccca gcgtttttca aatatttttt cttcttccat tattgatccc
     2281 tcgcaagacg ctacctcaaa cttaaataat gggcagcgaa agtctaattc cttagatcta
     2341 agtatagcaa acaaatatga aaaaaactta gaaggtagta aaagttcttc cgtcttttgc
     2401 tttgcttcta ttcttaatgt gtttcgttat cggtgcccta caaagatttt cattaattac
     2461 acactcattt attgttgtgt attttctgac atttctctct tgatcaatta ttttaattcc
     2521 atgggcatcc tgatagttca taggagagga gcggctatca gaaatagttg cacctatctc
     2581 ttgaacttct tttacaactt ctggaaaatc catccatcct gcttcagctg tcaaaagtaa
     2641 taatctataa tatttatctt tccttttcaa tctaagaaat cgtcacccca ttctttttct
     2701 tctattataa actatttaac ttttagaatt caaaaaccgt ttgtctattt ag
//
LOCUS       DAYYKC010000176         2676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000176 DAYYKC010000000
VERSION     DAYYKC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.182"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1400
                     /locus_tag="ACHBBW_002957"
     CDS             <1..1400
                     /locus_tag="ACHBBW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9154563.1"
                     /translation="GEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLP
                     ALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
     gene            1534..>2676
                     /locus_tag="ACHBBW_002958"
     CDS             1534..>2676
                     /locus_tag="ACHBBW_002958"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9154564.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFE"
BASE COUNT         1050 a          367 c          535 g          724 t
ORIGIN      
        1 aaggcgaaaa agtagaagtt gggggcacag tagatctaac agataatgtt actaacttgc
       61 cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca attgatacca
      121 acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt acgaaagata
      181 cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa tatgaaccaa
      241 cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat aatgttacta
      301 acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc ggtacaattg
      361 ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca gacggtacga
      421 aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca gataaataca
      481 atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca tctgagggaa
      541 ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag aaagtagatg
      601 ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat ggatcatctg
      661 atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat aaatataatc
      721 ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct gagggaatta
      781 agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa gtagatgtta
      841 gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga tctaaagaag
      901 aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct caagttgatc
      961 ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca gaggtgacag
     1021 ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt aattttacag
     1081 ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact gatgaatctg
     1141 gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac agtggaaaaa
     1201 acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa gacaaaaata
     1261 gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac agtaatatct
     1321 ttactattag cggtggttta atactgttag gaacgttagg attattaggg tataaaaatc
     1381 gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa gaggaagaat
     1441 ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta aagatgaata
     1501 tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat tattggttca
     1561 aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa ttatgaagtt
     1621 attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc gttatttata
     1681 aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac actaaattct
     1741 gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca aaataaaaag
     1801 attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa agttatatta
     1861 gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc taatattttt
     1921 tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga tagtactatt
     1981 aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga agccataaga
     2041 aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc gacttattac
     2101 gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat acatctttgt
     2161 ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat agtcactaca
     2221 agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct atctaatagt
     2281 ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac taatgaatac
     2341 cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac tttttttacg
     2401 tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac agcttatatt
     2461 gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt tacaaatggc
     2521 ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac agcacgatta
     2581 ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc ttttttgaga
     2641 aaaaatagtc gtgtaaaatt aagaattagc tttgaa
//
LOCUS       DAYYKC010000177         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000177 DAYYKC010000000
VERSION     DAYYKC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.183"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHBBW_002959"
     CDS             699..785
                     /locus_tag="ACHBBW_002959"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9154565.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHBBW_002960"
     CDS             complement(1087..1311)
                     /locus_tag="ACHBBW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154566.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHBBW_002961"
     CDS             1677..1961
                     /locus_tag="ACHBBW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154567.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHBBW_002962"
     CDS             1964..2380
                     /locus_tag="ACHBBW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154568.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACHBBW_002963"
     CDS             complement(2517..>2675)
                     /locus_tag="ACHBBW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9154569.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAYYKC010000178         2600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000178 DAYYKC010000000
VERSION     DAYYKC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.185"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2280
                     /locus_tag="ACHBBW_002964"
     CDS             <1..2280
                     /locus_tag="ACHBBW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9154570.1"
                     /translation="ISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSN
                     WSGLYFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMEL
                     LGQRIVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYS
                     TEGLSVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFK
                     TNLKLETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKD
                     VEQDGQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSL
                     DLNLIELLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEE
                     EYPTIFEIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSY
                     LDKKAEHYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESK
                     KRFPELSVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPS
                     RLREDILIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKR
                     DYDNRNSANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMW
                     VHDPENGNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELD
                     GLKQASSYLFKFKQKRSDG"
     gene            2415..2543
                     /locus_tag="ACHBBW_002965"
     CDS             2415..2543
                     /locus_tag="ACHBBW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9154571.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          994 a          340 c          498 g          768 t
ORIGIN      
        1 atcagtaatt ttgaagaatt aatagacaaa ttagatacga aagacattgt caattggctc
       61 aatcaagacg aagaattgac agatttatta tcttcggtaa aaaaagaatc aagtgcaagt
      121 cagcaatcga actggagtgg attgtacttt aattcaaatg agtatgtgaa atacattttg
      181 aataaaactg catggattaa gattgatgga gaaaaatata gcccgagaca aattgtgcta
      241 tatgaaaagc tccaggcaca cgttccggga ttgtatggag tttctgaaca aagcttaatg
      301 gaactcttgg ggcaacgcat tgttgaacag ttggatttca gaaaaaatat ggcgatgttt
      361 tcggatgatg aaataagagc tatattagtt gagttaccaa aattcgataa aggcgaaata
      421 agtcgcagat tatataatga acttataaga aataaaaaag gaatgaatcc tacttattct
      481 accgaaggat tatcagtctt agccaaagat ggcttctttt atccaaacaa agaattaagg
      541 tatgctgata agcgactgcc taaatcaata gaaaaacaaa tgcgttttat ttacgtgcct
      601 gaaaaagcta gtacaactac gatatatgaa tggcttgggg ttgaacgctt caaaacaaat
      661 ttaaaattgg aaacatttga gttattggaa gattattctg aggatttcaa aaaagagatt
      721 gatgatatca aaactgcggt attatctacg atagatgaca atagtaagaa tgttagttca
      781 ttaaaacgta ttcagattat tccatgcagc aaaatatctg ctaaagatgt tgagcaagat
      841 ggtcagtcaa tcgttttgga agattattat tttgtggaaa gtaaaggaaa attctactta
      901 aaattacctg ctaattctat tgaaattaat caattacgtt tgattgatac gttttcttca
      961 acaatcgtag acatattcaa gcaggtgtta aatctaagct tagatttgaa tttgattgaa
     1021 ttgttaattt cccgagatac ggaacataaa caaagaaaaa taactgaaga attcggtgta
     1081 gataagtgga atgaatcgtt tgagctattg tttagcgaaa atgcgttaaa taaaaaagtt
     1141 aatgactttt tgttatcaaa tggactaaat gaagaggaat atcctaccat ttttgaaatt
     1201 gactattcat atgcattaaa agaagaagag tatcaattgt tggcgatagc tctagaaagc
     1261 atttcaaaag atattgagga tttgaatgga ttgtcagaat tgattcattt ggatattcgt
     1321 gaattttgga aactgaaatt aagatcttat ctagataaga aagcggaaca ttaccgccag
     1381 attttatata gcaatgcaaa taaaagcggt gatgaaatac tcaaagaaac attcctagaa
     1441 aaaattgata gatacaaaga ttatcaattt gagatttctg aaattccaaa ttcagttcaa
     1501 gtcgacattg agaaagaaag taagaagaga tttccagagc taagtgtgtc aattactgat
     1561 gaaattgaca ttgatggcct gtatgataat aatgtcgaag agttagtcac ggttgcagct
     1621 attacaatag atgattttga ttattatatt agaggtcatc aaaaagtttc ttccttactg
     1681 tattttggta ttccgtcccg tttaagagaa gatatactga tattcgttac taaagaaaat
     1741 gatgttgatt tgaacggaga aaaatctggt atctctattg aaggaacatc aactgttcaa
     1801 accattcttg aaccatcgaa tagtgttagt aggaaaggta agaacacacg aaatggagaa
     1861 agaagtaaac gagattatga taatagaaac tccgcaaatg atgatgctgg tgaaacagca
     1921 gaacaaattg cttacgctga attgactcag aatcaaaaac ttaatgtaat ttggaatacg
     1981 aaatattcta gtaaatcttc agatcgtaat aagcagccac caaacggaat tgtttgtgat
     2041 atgtgggttc atgacccaga gaatgggaat atgtactttg aggtaaaatc atcaattaca
     2101 gaatttgaaa tgtcgattaa tgaatataat tctatgttag ataaccctga ctcttacgag
     2161 gtcgttttag tcaatcgtga taccaaagag ataagtcgtc ataaatttga tgaacttgat
     2221 ggattgaaac aggcaagtag ctatcttttc aaatttaagc aaaagagaag tgacggttga
     2281 gtgatttgtt tccacaaacc tattcaatct atcaactcaa aaatcataaa caaccattat
     2341 tatagtagga agatacagag tgtactctca acgcatatcg aattagtcgc ttcattcgtt
     2401 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
     2461 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
     2521 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
     2581 tattgtttct tagtcttttt
//
LOCUS       DAYYKC010000179         2547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000179 DAYYKC010000000
VERSION     DAYYKC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.186"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2195
                     /locus_tag="ACHBBW_002966"
     CDS             102..2195
                     /locus_tag="ACHBBW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9154572.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2220..2522
                     /locus_tag="ACHBBW_002967"
     CDS             2220..2522
                     /locus_tag="ACHBBW_002967"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9154573.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT          901 a          433 c          513 g          700 t
ORIGIN      
        1 gtttgctttg cttactttct ttccacttca agtggcagaa atatctactt tttattatgt
       61 aagcggaacc atatacttga aatcagaagg agatcatttc tatgaaaaca agaacaattg
      121 aaatacttca cagaatcatg acagcccaat atccagtaaa agcaaaacat ctagcaagtg
      181 agttcaatgt cagtcagcgg acgatccgtc aggaagttct tgaagcgaat atctggttga
      241 gcagtaaaaa gcttccggag atccgtacaa ttcgcaacaa aggttttttg ttgaagctgg
      301 aaaaggaaaa gcaagctcga ttagaaactg aaataaaaga tgtgaaagac ggctttttga
      361 atcggcgcga acgtattttt gatctagttt tagccgttgc ttacagccga atcccgatcc
      421 atctaaacaa aaaagaagaa gaatttcaaa tctctaaaag tacattagat gaagatatga
      481 gacggttacg agcaacactg gtcagttatg ggatagagat cgtcagttat ggaaagcaag
      541 gactggttta taaaggttca gaacgatcta tccgtacgat gatctatgat tttatcaatc
      601 agaatctggg aatgaccgac ttctttatga atcaagatgc acaattaaca ccggctcaaa
      661 aaatctttct ccattatttt cctttggtgg aaatcaaaaa aatacaggag atctattcca
      721 ataagttgtt gaaacaagaa gatgatattt ataaaaaaca aatcctgctt tttacattga
      781 tctggatcca gcgaatcagg aaacatgaat ttatctctgc cattaattgg aaaaatacag
      841 aaataaaaga cagtagcttt tctgcattta ttgaccacat gatcgaggaa tttcaattgg
      901 acaatgtccc gcaagtagaa agaaattatc ttcgtttcac tattgagacg ttcaatgcta
      961 gagatatctc caattcaatc gagtgggtac aagctcaatt actaacgatc caattgatcc
     1021 aatttgtaga gaacgagaca cacgtacctt tccatctaaa ggaagaagct ttattcgaaa
     1081 atctctataa gcacatggct gcactgattg taagaatcaa gaatcatatc caagtcatga
     1141 atccattgaa agaaaacatc cgaacaaatt atttcccaat ctaccgcgca gtcactcatt
     1201 ttgttcctac gatcgaagaa gtaattggtg gcaagatact ggaggacgag atagcttttc
     1261 ttgtcatcca cttttcgacg atcgccagtg taatcaagcg agacttgaat tatgtttata
     1321 aatcagtcgt tgtctgtaac catgggatgg ctactggaaa tctgttagca gagaatctga
     1381 aagaaaagtt tcctcagatc gagattactg cagttttaag ttcaaaagaa atcgaattag
     1441 tagataaatt ggatgtcaat ctggttttca gcacctttca attgaattat cgaaataagc
     1501 cgcttttagt tgtcgatccg attttaactg agacgaatat cttggttata gaagactttt
     1561 tgaaaagaaa tgaagaacta cagcggttag agcctaacag tcatgaatcg acggagttat
     1621 tcactcaaat aatagatgtc atgaaagaaa gtggtggccg agtagatcga atcatctatg
     1681 gcaagttaga aaaattattc gaaacaaaca atttagaaat caacaaaagg gagatccagc
     1741 ctatgttaaa agatattttg acggacagca atatcatgat caaagaaaaa gccactactt
     1801 gggaggaatc gattgaacta gctgctagac cattggttaa agaaaacatt gtcgaagagc
     1861 gctatgtcga agcgatgatt cgtgcagtga atgattacgg accgtatatc gtgattggca
     1921 aacacatggc actagcccat gcccgaccag aagatggggt caataaatta ggagtcagcg
     1981 tagctacgat tgagcaacca atcgatttcg gtaatcctga aatggatcca gtaaagatta
     2041 ttttttgttt ggcagcagta gactcctact cgcatctgaa catcatgaaa gaactaattg
     2101 aattgatcaa caacgaagag aagttggatc gattgattga atgcacaaat atcgaagatt
     2161 tcaaacaact gcttttttca gtagttgaga aataataaat gatacggggg agaacaaaga
     2221 tgaacaaatt gaacatttta ttcgtatgcg gtgcaggttt aggaagtagt tttgcagcac
     2281 agatgtctgc tgaggatgtt ttgaacaaat taggagtaga agccaaactg gaccattgtg
     2341 atatttcttc cgcagtttcc atgaaaccag atgtgatcat cacagcagaa aattttcgtt
     2401 cgcagttcga aaagttcgca gttgatccta aaacggcgat gatctatttg aaaaatattg
     2461 tttcaaaaaa tgaaattgaa gaaaaaatca ccccagttct gcaagataag ggagttatct
     2521 aaatacaaaa agaaaagagg gtaaaaa
//
LOCUS       DAYYKC010000180         2538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000180 DAYYKC010000000
VERSION     DAYYKC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.187"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..2469
                     /locus_tag="ACHBBW_002968"
     CDS             28..2469
                     /locus_tag="ACHBBW_002968"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9154574.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          840 a          455 c          603 g          640 t
ORIGIN      
        1 ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga
       61 acgccgatag gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca
      121 cttgtgacta aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta
      181 atatttggaa atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg
      241 aacagcggtc tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt
      301 atgaaagcag tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata
      361 gccggcggaa cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca
      421 aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca
      481 aatgcacata tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa
      541 gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt
      601 gtattttctg aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat
      661 gaagcagttc gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca
      721 gaagaaggaa ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg
      781 atccttgcat ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa
      841 gaagtagcgg aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt
      901 caaaaattga cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat
      961 gaagcatttg cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag
     1021 gtcaatatat atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt
     1081 atcttgacta cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca
     1141 ttatgtattg gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca
     1201 cataaggatg tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa
     1261 ttaatagaaa aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt
     1321 tctgaagagc tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg
     1381 gggatcgcgc agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag
     1441 gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca
     1501 gaaacacctc agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa
     1561 gcagtgatca atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt
     1621 tatccttcaa tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg
     1681 gggagtttcc atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc
     1741 gctaacatga tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct
     1801 gaagaagaga ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc
     1861 tgttgtgaaa tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc
     1921 aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac
     1981 aacaaaggga tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt
     2041 gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca
     2101 gactggcaga tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc
     2161 acagtaggcg gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat
     2221 attgattcag ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg
     2281 gctgctttgc gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct
     2341 cgttcattag cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa
     2401 ttacgtgaag cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg
     2461 gaaaaataaa aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta
     2521 ctgcagatct tgcttttt
//
LOCUS       DAYYKC010000181         2426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000181 DAYYKC010000000
VERSION     DAYYKC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.188"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2276
                     /locus_tag="ACHBBW_002969"
     CDS             123..2276
                     /locus_tag="ACHBBW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9154575.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          829 a          426 c          536 g          635 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAYYKC010000182         2366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000182 DAYYKC010000000
VERSION     DAYYKC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.189"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBBW_002970"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBBW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9154576.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1227..2330
                     /locus_tag="ACHBBW_002971"
     CDS             1227..2330
                     /locus_tag="ACHBBW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9154577.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          737 a          463 c          419 g          747 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca tataataatc
      961 ttaattttta gaatttttag ataattacaa aaaagcaaag ggaatcggta gaaatactaa
     1021 gaggaacaaa aattcattat tgtcgttttc aatgtttttg tacttgttcg tttttaggat
     1081 acttattgat gatttcaagg tgttcatgcc accttattca tcacttatcc tgtcttaggt
     1141 tcacctctac tccatctaca gccgcagtca gatcgatcac tctttaaaat tttaacccgt
     1201 ttcaacgaag aaagaaggaa atgaatatga gtcaaagtgt tttcttacca aattatacca
     1261 ttggaacaga tgcgtatgaa aaagcaaatg gaatcgtcaa agaatatggg acaaaagtag
     1321 tgctgattgg agggaagact gcactttcaa aggcagaaaa caaaataaaa gtagctttga
     1381 cacaggaaat agaggttgtc gccagtctct ggtacggggg taattcaact tatggaaata
     1441 ttgaacagtt gatccagcat gaaagtgtcc aaaaagcaga cgtcctgttt gcagtcggtg
     1501 gtggccgtgc ttgcgatact gtaaaagtca ttggtgaaaa actagctaaa ccagtcttca
     1561 cctttcccac tattggttcg aactgttcgc ccgtcactgc ggtatgtgtc atctataacg
     1621 aagaaggcgt aatggacgga ctcttctttc cttcaaaacc acctgtacac accttcattg
     1681 atacagcaat tattgccgaa gcacctgata aatacttatg ggcaggtatt ggcgatgctt
     1741 tatccaaaga agtcgaatct actttttctg ctcgtggcga tgatctaggc tacacggata
     1801 gattaggaat ccaaatcgga aaaatgtgca ttgatcgtct cttgacttat ggtacgcaag
     1861 cattgaaaga tagtgccgac gatcgtccct catatgcaat tgaacaagtc gtttcggata
     1921 tcattgttac taccggattg gtttctgttt tagttaacaa taactataat tctgctatgg
     1981 cacactcatt ctattacggt acgactgttc tagaaaaaat ggagggatac cttcacggag
     2041 catctgtcag ttatggtgta ctgaccttat tgatgatgga tcagcagttc gatttgtttg
     2101 ataaaatcta tgtgtttatg aaaaagaacc aacttccagt atcgttaacc gacatgaatc
     2161 tcaatgtcac gaccgattta gaagcaatct tagataaagc catggcaaca aatgatatta
     2221 aacatgtgcc ttatgaaatc acccgagaaa aatttaaaca ggccattctt gacgtagaaa
     2281 cttatcatgc tacacaagaa agccagcaag gatataagaa aatcagttaa ataattgcta
     2341 aaacccccat caagcaggct gtcatc
//
LOCUS       DAYYKC010000183         2360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000183 DAYYKC010000000
VERSION     DAYYKC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.190"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..412
                     /locus_tag="ACHBBW_002972"
     CDS             <1..412
                     /locus_tag="ACHBBW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9154578.1"
                     /translation="TNEDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDF
                     KALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPG
                     RPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            449..1459
                     /locus_tag="ACHBBW_002973"
     CDS             449..1459
                     /locus_tag="ACHBBW_002973"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9154579.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1574..2287)
                     /locus_tag="ACHBBW_002974"
     CDS             complement(1574..2287)
                     /locus_tag="ACHBBW_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154580.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          793 a          392 c          486 g          689 t
ORIGIN      
        1 tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa
       61 aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa
      121 acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc
      181 agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga
      241 aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc
      301 accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca
      361 gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa
      421 aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc
      481 aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga
      541 atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg
      601 gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat
      661 gatgcaagat gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga
      721 gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga
      781 tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac
      841 caaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt
      901 actgaaaaca agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga
      961 gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca
     1021 atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac
     1081 gatcagtcat ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa
     1141 tgatggaaag ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag
     1201 aagatcactt gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt
     1261 cggtagaagt gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct
     1321 gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca
     1381 actgacattg atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt
     1441 tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa
     1501 tctaaataga aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga
     1561 tctattagaa attttaatta tccataacta tcacattgat cagctgtttc acatcatttg
     1621 gattacctgt aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc
     1681 taatgaacgc tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat
     1741 aactagtgat tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt
     1801 tcccagtgag cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga
     1861 taggtgttcc tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa
     1921 aagtattacc tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc
     1981 ctgctaacgg atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag
     2041 aaatatttgt ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac
     2101 tgcttgttga agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag
     2161 ctgaactggt ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt
     2221 tactaggaga cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact
     2281 ttttcatata aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt
     2341 gaatccgtaa aaccttgtct
//
LOCUS       DAYYKC010000184         2346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000184 DAYYKC010000000
VERSION     DAYYKC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.191"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..2017
                     /locus_tag="ACHBBW_002975"
     CDS             155..2017
                     /locus_tag="ACHBBW_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9154581.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2017..>2346
                     /locus_tag="ACHBBW_002976"
     CDS             2017..>2346
                     /locus_tag="ACHBBW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9154582.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          842 a          349 c          459 g          696 t
ORIGIN      
        1 aacgcttttt aacagtacat gtagtcgtct ttataataga taaactctgt aatattagtt
       61 aaaaagctat aagttgcact tagttaagtg caaacatttc atattgtcgt tagtttagaa
      121 aacgcttata ttagcaatgt aaggagagat gataatgcag cagctaacaa aaagagaaag
      181 gcagcttttg atttacttgg tggaaatgtc tgaaccggta acaatcaaaa agttggctga
      241 aaaagaaaat gttagtgttc ggacgataaa atacgatcta gatgatataa gagagtggct
      301 gaatgagcgg aagcaagtcc tatatagcaa acgtagtcag ggaatttgga tgaatattag
      361 tagctctgat cgaatcaaat taaaaagcga attgatggat gttaatcgtt ttgaaatatt
      421 tgcagatcaa agtctccgaa tcaaacgttt actaattatg cttttagtaa caaaagaagc
      481 tattactgga atgaaaatgg ccaacgcatt agatgtcagc aaagatacga tcatgaatga
      541 tttagatgtg ctggaaaata attttcttaa ggaaggattg accttgaatc gtcaggcgcg
      601 aaagggtttt tggatcatag gagaagaacg tctagttcga ttaacgattg aagagattct
      661 tcaaaaagag ttcacggatt atgacattta taaattaatg tcattgttat tgaatggcgg
      721 tgagactgaa tactttgaaa tgtattctgc tactgctaca cctatccaag aggtatttaa
      781 tcaagtaatc attcgaatgc gacacttact ggaatttgaa aatctagaga aactgaatta
      841 cgcggaatta ctaaatattc tgattcgagt gacaattgct accgttcgct tacgcaaaga
      901 ggctacgatt ggtcgttatc aattggttgc tgaacaagaa ttagacaaaa acgatctttc
      961 ttatcgcgtc atgcagcagg tctttgatta ttatcagttg ccactgttcg aggatgaata
     1021 tcattatatc tatagtgata cattcgacac gattcaccag caagacgtca tgaaactagt
     1081 tgagaatgtg atttataaag taagtaaagc aatcaaccat tctttttatc aagatccaca
     1141 attgttgacg aatctgtatg ctcatttatc catgcgatta tcgagaaaga aaaaatttgt
     1201 caatgaatat aatcctttta aagaagatat caagcagaaa tacccggaat tatttgatgc
     1261 gatcaagcaa gcagttcgac aagaaataag tgaacaaaat attctgatca atgattcttt
     1321 cgtggcatat attgccttac actttttggt ttcctacgaa agagaagaag atctacgaag
     1381 cgttagagcc gtctacattt gttcaacagg gttgggtgtg actagtctga tcaagcaaaa
     1441 gatcaatgaa gaattaaata atatcactat cgtggcattt gcttcagtat tgaatgcgca
     1501 agaaatgatt gctaccaaag atcctgattt ggtgattagt atttttccaa ttaatggcat
     1561 cgatcgtcct ttcatcaaag tccatccatt acccactgaa caggacttga aaaaaattca
     1621 acaaataata aaaaaaatac ttgcttcttc caatcagcag gaaaggcata acttgaatct
     1681 tagagaaacg tccatcaata aagatacttt agaagacttt agcaaggaat tgattttgaa
     1741 agcttatacg atttatgaac gtttacttgt cattttggaa gcagaattga tccaagaata
     1801 tcgagaagct tttttgcttc atgtattctt aatggttcat cgtatcacat ttgatcaaca
     1861 atatatggca gaaggtcgtc aagtagacca gattatttta gacaaaggaa cactggtagg
     1921 aaaaatcgaa gcagtttttg cagaaaataa tttaggaatc aatcagtcag aaatcagtgc
     1981 attattctca tatataaaag aaggagcgtc aagttagtga aaatgtcaga cgaggcttta
     2041 aaaattatcg aggaaagtca aaatcgggaa aagttaaaac aagtgattga tctaattgaa
     2101 gagcgattag tagaacatgc tattgtacca accgagttgc agtggaccat tctaattaat
     2161 cacttgaatg agatgctgaa acgttctcaa agaaaggaaa cgattcccgt agtcgatcgg
     2221 gatttgttca aagaagtatc caaagaagcc ataactattt ctgatgaaat agttagaagt
     2281 atcggtaatt taaccgatga tgaaatatat gttttgtcga ttcattttga aacagcaaaa
     2341 aataat
//
LOCUS       DAYYKC010000185         2270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000185 DAYYKC010000000
VERSION     DAYYKC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.193"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHBBW_002977"
     CDS             191..445
                     /locus_tag="ACHBBW_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9154583.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHBBW_002978"
     CDS             446..763
                     /locus_tag="ACHBBW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9154584.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHBBW_002979"
     CDS             1322..2257
                     /locus_tag="ACHBBW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154585.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAYYKC010000186         2251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000186 DAYYKC010000000
VERSION     DAYYKC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.194"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1698
                     /locus_tag="ACHBBW_002980"
     CDS             85..1698
                     /locus_tag="ACHBBW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9154586.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          707 a          400 c          444 g          700 t
ORIGIN      
        1 aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat
       61 caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt
      121 ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa
      181 cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc
      241 actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc
      301 gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt
      361 aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct
      421 ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc
      481 ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa
      541 catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat
      601 cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca
      661 gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa
      721 acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc
      781 ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa
      841 gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc
      901 aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt
      961 cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat
     1021 attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc
     1081 tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata
     1141 acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct
     1201 acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa
     1261 gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga
     1321 aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact
     1381 acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta
     1441 gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat
     1501 atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg
     1561 aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc
     1621 agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg
     1681 attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt
     1741 tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata
     1801 gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag
     1861 aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt
     1921 tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat
     1981 tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat
     2041 tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg
     2101 cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt
     2161 tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata
     2221 atttaatttt tttgttccta tttatttaac a
//
LOCUS       DAYYKC010000187         2121 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000187 DAYYKC010000000
VERSION     DAYYKC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.195"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..709)
                     /locus_tag="ACHBBW_002981"
                     /pseudo
     CDS             complement(102..709)
                     /locus_tag="ACHBBW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1232..1906
                     /locus_tag="ACHBBW_002982"
                     /pseudo
     CDS             1232..1906
                     /locus_tag="ACHBBW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1899..2060
                     /locus_tag="ACHBBW_002983"
     CDS             1899..2060
                     /locus_tag="ACHBBW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154587.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          761 a          332 c          362 g          666 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta a
//
LOCUS       DAYYKC010000188         2103 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000188 DAYYKC010000000
VERSION     DAYYKC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.196"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..818)
                     /locus_tag="ACHBBW_002984"
     CDS             complement(<1..818)
                     /locus_tag="ACHBBW_002984"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM9154588.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTILRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLA"
     gene            complement(790..1686)
                     /locus_tag="ACHBBW_002985"
     CDS             complement(790..1686)
                     /locus_tag="ACHBBW_002985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9154589.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1676..>2103)
                     /locus_tag="ACHBBW_002986"
     CDS             complement(1676..>2103)
                     /locus_tag="ACHBBW_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154590.1"
                     /translation="IGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVILDKCHFGL
                     NLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGINYNSTVDENEMLKF
                     YQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          734 a          356 c          254 g          759 t
ORIGIN      
        1 gctaacatat tgatagaaaa tatatatgag cttaagctga taactttttt ttcgtaaata
       61 ttcatttttg ataatttatg aactaaaaaa agatttgctg aaaaaaagag agcaaacatc
      121 actacgtgag aaatcgatgt actgtttcta tcaaaatatg catcatactt cgtatttata
      181 aatattgaac tgatataact ttgtccaata gagaatagga gcaagctaag gattgtaaca
      241 tatccagtaa tctttttatt aatttttaca ataaaaaaag ggagataaaa atatgcaaga
      301 ttatggaaca gtccagccac taagataaaa aaaatatact taaatatagc tttgctgcct
      361 cgcaatatag ttagatgata aaaagctagt aatatgaata tcaatgcgct aaacatacgt
      421 acttgtattg cttgtgaagt aaaaccaaaa ataaaaaaca agaacaaaac taaaaaaaca
      481 ttagttgtga ataatctgat gacatacaat aaagctgaga gtaagacagt agaaataatc
      541 aataaaaaaa tctgatactg actggaaata aaggtacata cgtacataac agctctgaat
      601 cccggttcaa tactatctga actaaagtcc ataccaaatt gtgctatccc attatacaag
      661 acagaatatg cataataatc agcgttttgt gtgttaaatg ccatgatgat ccacattatc
      721 aaaagtagca ccccagacaa aatcttgttc gatgtactcc attttttttg tgaaacactc
      781 ttttcttgat taatctccaa tgacgcctct gtatccatct cgaacccccc ttatagccgc
      841 tctaaatcta ctgagcttat cagtctcaaa agctattgca ataaaaaatt tcaataatct
      901 tttattcata aggcgggaaa attccttatc acttttatac attcttttca ccaatgttga
      961 atttcttgca ataaaatact ttcgtgttga attatgattg tctattttaa ttttttttga
     1021 tcctattttt aaattttctc catctccaac gtgatgataa aaatagactt gatttgttac
     1081 ataaatgtta tagccattat ttgcaaccct tttacaaaat tcaacatcaa tctgatcaat
     1141 aaaaaattca tccttcatat ttccaacatc gtcaattaat tcacttttga aaaacattcc
     1201 agaggaattt gcaaatgcta ttttcatgga accactagtt gaagcaaagt gtttttttac
     1261 gtatctccct ctctcatttt tgcaggtaaa agagacagat tcatcattgc tattctcaaa
     1321 gtaattggga acaataatcc caatgtcctt atctttcgct tcaatattgg taaactcttc
     1381 taataatttt aaagaaagat tcttttgtat gatggtatct tgatctagta aaaggatata
     1441 atcacatttc tcttttttgg caatttcaat acctatgttt tgagctgtcg caagcccaat
     1501 gttctcattc atttttttta aaattaagtt ttccatcatt tcaaattgaa aagagttaat
     1561 attatctgag ccgttatcca cgataataag tttgttctgt ttctcttgta ttaacgtatc
     1621 cactaataac tttatgttat tatctggatt gtaggtaaca ataattatcc caattttatt
     1681 tttcatcgtt catcaccttg tacacttcat tccatttcat tttcaatact tgctcagaga
     1741 atatagtggc gtaaattttt ctaacatttt catttatgtt tttattacac tgataaaatt
     1801 tcaacatctc attttcatca acggtactat tataattgat tccgacatta tatcttttaa
     1861 ctatttcttt cgtatctcct gataaatttg aaattatagg taatccaaac ctaaaatact
     1921 caatcgattt cagacttaaa ccaattatgg tatttgactt aaataaattt agtccaaaat
     1981 gacatttatc gagtataact tttttttctg cattgtcata tataacaccg cagtcaacaa
     2041 attcaatatg atttttttca caaatatcag ctaatttagt tttgctttca ccttttccaa
     2101 taa
//
LOCUS       DAYYKC010000189         2044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000189 DAYYKC010000000
VERSION     DAYYKC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.197"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..623
                     /locus_tag="ACHBBW_002987"
     CDS             <1..623
                     /locus_tag="ACHBBW_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9154591.1"
                     /translation="GAGGHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELN
                     SWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVI
                     FANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGS
                     QCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            574..1755
                     /locus_tag="ACHBBW_002988"
     CDS             574..1755
                     /locus_tag="ACHBBW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9154592.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1768..>2044
                     /locus_tag="ACHBBW_002989"
     CDS             1768..>2044
                     /locus_tag="ACHBBW_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9154593.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEI"
BASE COUNT          728 a          268 c          423 g          625 t
ORIGIN      
        1 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
       61 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
      121 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
      181 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
      241 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
      301 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
      361 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
      421 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
      481 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
      541 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
      601 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
      661 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
      721 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
      781 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
      841 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
      901 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
      961 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
     1021 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
     1081 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
     1141 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
     1201 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
     1261 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
     1321 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
     1381 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
     1441 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
     1501 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
     1561 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
     1621 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
     1681 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
     1741 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
     1801 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
     1861 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
     1921 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
     1981 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
     2041 atca
//
LOCUS       DAYYKC010000190         2041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000190 DAYYKC010000000
VERSION     DAYYKC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.198"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            654..986
                     /gene="traE"
                     /locus_tag="ACHBBW_002990"
     CDS             654..986
                     /gene="traE"
                     /locus_tag="ACHBBW_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9154594.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1605..1946
                     /locus_tag="ACHBBW_002991"
     CDS             1605..1946
                     /locus_tag="ACHBBW_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9154595.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          713 a          271 c          429 g          628 t
ORIGIN      
        1 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
       61 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
      121 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
      181 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
      241 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
      301 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
      361 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
      421 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
      481 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
      541 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
      601 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
      661 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
      721 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
      781 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
      841 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
      901 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
      961 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     1021 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     1081 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     1141 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     1201 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     1261 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     1321 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     1381 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     1441 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     1501 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     1561 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     1621 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     1681 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     1741 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     1801 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     1861 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     1921 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     1981 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     2041 t
//
LOCUS       DAYYKC010000191         2023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000191 DAYYKC010000000
VERSION     DAYYKC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.199"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1294
                     /locus_tag="ACHBBW_002992"
     CDS             125..1294
                     /locus_tag="ACHBBW_002992"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9154596.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1561..>2023)
                     /locus_tag="ACHBBW_002993"
     CDS             complement(1561..>2023)
                     /locus_tag="ACHBBW_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154597.1"
                     /translation="MVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQ
                     VTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGP
                     FVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          721 a          341 c          305 g          656 t
ORIGIN      
        1 aatattctat caaaaatgta agtagtaaat ccattactat aagattaaaa taaatgttat
       61 caagatacaa gctattttag ctttattctt tctgtaaaaa agaaaggaga ctaaataaac
      121 tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat tcatttttaa tgatcgttgt
      181 cgtgttgttt agccttagtg cgttctctac aggaactatt cttggatggc aattttacaa
      241 taatgaacac tctcagatag ttgcccatgc tcaatcagct caaaaaaagt tgatactgac
      301 taccaatgaa caacagctcg ttgaaaaaat cgatcaacta ttaagaaaaa atgattatat
      361 tggctcaatt tacgtaaaga aaaataatcg catcatcatg gaaaaaggct atggctatgc
      421 gaataaactc acgcaacaac caaatgatcc ttctctttat tatcagattg gttcggttca
      481 aaaagcattg actactcttt tgattttaaa acaagtcgag cgtgggcacc tttctttaga
      541 tactaagctg gctcaattct atccacaaat tgctggcagc cagcaaatta ctatccgtga
      601 cttgctgtat atgcgctcag gcttgcaccg aactgcttct ccaaccatac cgatgactga
      661 tgaagaagtc gttcaatttg cgattcagca cttaaaattt acagattatc agacctctca
      721 ttatgattca ttgaacttta ccatattgac tggaattttg atcaaattaa caaatcaacc
      781 gtatgaaaaa ttattgaccg atgatatcat cgaaccttta ggattacagc atacaaaatt
      841 ttatgaccaa gtcaaaaatt catccaatca tgcactatcc tatcaaatgt ctaaagagga
      901 tgattacttt aaaccactga cagaatctga aacagagatc cataacgaat tagggactgg
      961 taatatcagt atgtccgtct atgatttaaa tacgctgttt acacaagttt tatctggaaa
     1021 actagtttcc aaagacttac tcttctcatt atggcgtgaa aatgcgaatg gacatccata
     1081 tagtggtggg gtatatagcg gagataatta tatcctgtcg caaggaaata tcgactcttt
     1141 tgatacatct gctatattta gcaaagattc acaggatgct atcatcatgg aaagcaatat
     1201 aaaagctgac aaaactatca aactgcctac tgctgatcta agaaaccaaa tctatgacct
     1261 gatggaagga actactaagt tggcaaatag ctgatatgat gaaaagatag caaatactac
     1321 ttctttgaat agcatttgct taaaaaaagt actcgactaa acgtaggaaa gagtgtggga
     1381 caaatctaaa aatcaatttt tgctacagac tttaattcca aataaacaga acagaaagag
     1441 gtgttcttct ttctgttctg tccaaattat tctaagattt ctctttacca acattatttg
     1501 acaaagggcc tattaaatct aaaatagtat ctttagattg ctaaaatatg tgctagcaaa
     1561 tcattcatgg cttgttctta tttttaattt tatgcaataa aaaagaagaa caattagcgt
     1621 gattatagaa ataaataatg agattttaga aaaaaatgtg tatgaatacc tgattgttac
     1681 tttatttatt ccactttcac tttttactgt aacaaaagga cctccttgtt ttagtactgt
     1741 agcatttttt gaattaacag taaccgttaa tcctttatac cggaatacag gtaaaactat
     1801 tgttttgcct tttgaagatt taaacgtaaa tgtaatttca tctttatttg gagaaaagct
     1861 actataaata acttgaccat cttctgtaac ttgatggttt ttcaaaagca attgatcttt
     1921 tttatagcct ataggtacat aatcaaaatt ctcaaattct tcaagtttat tttccatttg
     1981 ttcattagaa taagttcctt gaaaattcat ttctttcacc atg
//
LOCUS       DAYYKC010000192         1926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000192 DAYYKC010000000
VERSION     DAYYKC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.200"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            610..1779
                     /locus_tag="ACHBBW_002994"
     CDS             610..1779
                     /locus_tag="ACHBBW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9154598.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          724 a          264 c          296 g          642 t
ORIGIN      
        1 tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa
       61 aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga
      121 tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc
      181 aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata ataaagtact
      241 acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac tatattttag
      301 gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa tttaaaggta
      361 ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt aataagagtt
      421 tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag tgatataacg
      481 aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga tatttagggt
      541 agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat tttataggag
      601 gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa taaaggtgat
      661 ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt gcaggctgaa
      721 ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc
      781 cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa tttggtaatt
      841 cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa aagaaccaat
      901 gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat gtctgaaatc
      961 tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat ttcaaatatg
     1021 actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga atccagatac
     1081 tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa
     1141 gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct tgggtttagt
     1201 cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct aaaagaccgt
     1261 ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa cgatagtatt
     1321 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
     1381 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa attagtttat
     1441 caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga taaatggaaa
     1501 aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac cttttataat
     1561 ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg ttttaacaaa
     1621 caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc tctggagaga
     1681 tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt tcataaaggt
     1741 tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca gaatgggctt
     1801 ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg taaagttata
     1861 aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat tcatatgcta
     1921 atttat
//
LOCUS       DAYYKC010000193         1925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000193 DAYYKC010000000
VERSION     DAYYKC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.201"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHBBW_002995"
     CDS             complement(<1..426)
                     /locus_tag="ACHBBW_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9154599.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHBBW_002996"
     CDS             complement(535..1566)
                     /locus_tag="ACHBBW_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9154600.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          591 a          321 c          333 g          680 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taatt
//
LOCUS       DAYYKC010000194         1918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000194 DAYYKC010000000
VERSION     DAYYKC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.202"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHBBW_002997"
     CDS             complement(139..1338)
                     /locus_tag="ACHBBW_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9154601.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..>1918)
                     /locus_tag="ACHBBW_002998"
     CDS             complement(1468..>1918)
                     /locus_tag="ACHBBW_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9154602.1"
                     /translation="FAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVF
                     ISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTL
                     SSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          605 a          383 c          315 g          615 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaat
//
LOCUS       DAYYKC010000195         1914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000195 DAYYKC010000000
VERSION     DAYYKC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.203"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..80
                     /locus_tag="ACHBBW_002999"
     CDS             <1..80
                     /locus_tag="ACHBBW_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9154603.1"
                     /translation="PGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            77..>1914
                     /locus_tag="ACHBBW_003000"
     CDS             77..>1914
                     /locus_tag="ACHBBW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9154604.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          717 a          234 c          383 g          580 t
ORIGIN      
        1 atcctggaaa aaatgaaatt aaatggacga aaaatattca ttcatttgaa ataatgccta
       61 ggtggtggag aaaagtatga aaccaagaat atatagtcct actgaaacag atttctcaac
      121 gaatggctta ggaattttaa aggattgtac aagatgtgaa atatatgaag tagctaatgg
      181 aaaatatgaa ttagagttgg attatccttt aggaactaga tttgatgaat attttgaaaa
      241 tgactatcaa ataaaagcaa agccaaacga tcaagaagag tatcatatct tttttattga
      301 tgataaagat atagatactt ttttaaatac agtaactatt tatgctcaga gtcgtacaaa
      361 tcgacttgga agacgggtag tcactcttgt ggaagtggac tctaaaactg gtcaagaagc
      421 gatatcaatt attgaaacta aaatggataa aaaatctgac atacgacttt attctgatat
      481 tacggctgtt tctagcacaa tctttgaagc gagaaatgtt ttaaattgta ttgctggtga
      541 acaaggatca ttgcttcaat attggggcgg agaaattaaa cgtgaaccat ttaagctttc
      601 tttgttaaag cgaagaggtc gcaataatgt tggaacgatt cggtatggaa aagatatgtc
      661 tggtttgaag gtcaaattag attggacagg tataaaaaca agaattattc catatgctga
      721 tcctcaaagt gaagtaggta cgactaatcg aatttatggt tcgccagtag atagtgccta
      781 tattaataac tatcctgatg tctatacaga acatgttcag tttacagaag aacaaggagt
      841 aaaagacatt aagagcttaa ataaaatagc taaaaattac tttaaaacga tcaatcctgg
      901 ctgtgataag cctaaagttt ctattacggt tgaatttgat aagttgactg atagtgaaga
      961 agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt gatacattta aaatatatca
     1021 taaaaaatat aatatttatc ttgaatcaaa ggttagtgga ctacaatacg attctttatc
     1081 tgaaaaaact ttgaaattag aagcaggaga cattcaagtt gctttttatc agcaacaagc
     1141 tgtaactatt caggataaat taaaagatta tgcaacgaat aattatatga gtgattttaa
     1201 tgattatgtt tcttcaatga ttacaggtca aggcagtgca ggaggatatg tagttttatg
     1261 gccaaaagaa aaaccttcca acatctttat tatggataat ccagatttag aaaaggcaag
     1321 agaagtacta agaatgaata aaaatgggat tgctttttct aagaaaggat ggcgggggcc
     1381 ttttaattca gcttggacat tagatagtat atttaatgcc aattttatac aaactggatt
     1441 aatcaaagca gatatctttc aaaattcttt taacaagact ggagatgtat tgaaattagt
     1501 taatggacta ctccaaattt ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg
     1561 aatggaattt tggaattcta agggttcaat tggaacgatt ggaacaactg attctgttgg
     1621 taatcctttt cctggggctt ctactccaac ccctattgaa gataattctt tagttattcg
     1681 tacaaatgga gacggcaaat atattttgat ttctcctaaa gtaggaaaag gattagtttt
     1741 attaggaaat ggtaaagcaa tttattttgg agacttagat gtacaaggaa aacttacagt
     1801 taaaggaaaa gaaataattc ctggacaagg tggtggtcca tcgggaggtg gagaatctac
     1861 tggtgggtat cctagtgaat taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DAYYKC010000196         1908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000196 DAYYKC010000000
VERSION     DAYYKC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.204"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..425)
                     /locus_tag="ACHBBW_003001"
     CDS             complement(<1..425)
                     /locus_tag="ACHBBW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9154605.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
     gene            complement(426..1334)
                     /locus_tag="ACHBBW_003002"
     CDS             complement(426..1334)
                     /locus_tag="ACHBBW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM9154606.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1334..>1908)
                     /locus_tag="ACHBBW_003003"
     CDS             complement(1334..>1908)
                     /locus_tag="ACHBBW_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9154607.1"
                     /translation="KKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELP
                     WIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLT
                     IIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEF
                     FTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          639 a          334 c          211 g          724 t
ORIGIN      
        1 actgaaaagt agacgtcttt atctaaattt tttagatgtt catctattaa ttcttcaaga
       61 ttagaaactt cacgtaaagg tgaagtcaaa tctaaatcta taattaaatc aaatatatta
      121 tcttctcttt ctagttgtaa gaatgaatca cgtatcactt caattttccc tacagtatct
      181 gtagctaatt caggtttacg ttctacaatt ttcacagacg gatttaactt agtcgtcaat
      241 tctactaact cattactgtc cgtattcaaa caagtcgtgc aattatgttc aggatgtttt
      301 tgttgataga tatctatcat tgctaaagcg aaatggacta ggggctttcc tgccaaatat
      361 ttcaaatttt tatttttaaa ccccttagac ccagctctac cacaaatagt aaataaaatt
      421 tccatttatt tctcctcagc tattttcaat acttcgtttg catgatataa atcattccag
      481 ttttctttgc ctgttactac gttttcaaag aaatatttca actccttctc atacatatca
      541 ttagcagatt caggcatttc tttaacaatg ttttcttcaa aacttatttt attatttaat
      601 aagtcaacat tgatacattc atctaaagta aacaattgaa attttcttgt aggaactctt
      661 ccaaaataat ctaaatgaat ttctataatt ttatcatcgt atctcaatat atatgttgct
      721 aaatcattac tgttaatttc aagattcgaa tatttaccaa actgtttttt tactgattta
      781 gggaaaccaa ataggtttat aatataatcc aattcatgta tacagtctaa ttctactcct
      841 ccccccatag atttaaaggc agaataatta tttctataat cagtaccttt tctccaatca
      901 ggaagataag tagaacaaat agctcttaca gaatatactt ttttactttg aatactatta
      961 cgaacaactt ccatgatatt tttatacctc agtggagcag caacataaat atttttgtaa
     1021 tcataattat attgagtaag atcaatactc tcagaaaaag caggtttttc taaaaatatg
     1081 taatcaactt tttcaacaat tacatttagt gtttccagat gcttaaaagt cggatttgta
     1141 ataaaagcta tatcataatg tgtatccaaa tcgtctctat tttgaatata cgttatttct
     1201 tcaaactctt tttttccact gctactataa gctgttatat caaaattaat agaattcttt
     1261 tttccatagc ttattagatt ttttagatgt ctctttccaa ttgaccctaa cccaaaaaaa
     1321 attactttca tcattaattt acctcaatgt cgttaattaa gttaataata tacttatctt
     1381 tttcaaaagt atattggggt tccattttct tttccaaaag cgtgaaaaat tctttaatct
     1441 cctctaaata tgcatcttca ataatagtat gtgaataatt attatcatgc ataacctcat
     1501 cgtacaattg aatatctgtc atactagaag tttcttcatt ccatgaaaat agagaatccg
     1561 ggttaccttc ccattctatt aaaatctttt caccaattat agttaaattt cttttagcta
     1621 ctcttgaaac aacatctaca cttaaagttc caactatacc agtttcatgc tcaataataa
     1681 gagtgaaact gtccgaataa tcaatatcaa gattggataa tttctgggtc attacagtaa
     1741 acgatttaac tttcccaaat gtatctataa tccaaggtaa ttcaatagcg aatatttctc
     1801 tacacccatt agtttttttg tttgaaacaa aaaaattgcg ataatcttcc caaggatgcc
     1861 agtcgggtaa atactgtcct acatggtatt gataagaaat ttttttat
//
LOCUS       DAYYKC010000197         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000197 DAYYKC010000000
VERSION     DAYYKC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.205"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHBBW_003004"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHBBW_003004"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9154608.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYKC010000198         1845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000198 DAYYKC010000000
VERSION     DAYYKC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.206"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..709
                     /locus_tag="ACHBBW_003005"
     CDS             <1..709
                     /locus_tag="ACHBBW_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM9154609.1"
                     /translation="PFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLS
                     TGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDH
                     SLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGD
                     GVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKK
                     VKRPISADSVLLEEDVE"
     gene            706..>1845
                     /gene="neuC"
                     /locus_tag="ACHBBW_003006"
     CDS             706..>1845
                     /gene="neuC"
                     /locus_tag="ACHBBW_003006"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9154610.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQI"
BASE COUNT          679 a          240 c          361 g          565 t
ORIGIN      
        1 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
       61 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
      121 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
      181 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
      241 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
      301 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
      361 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
      421 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
      481 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
      541 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
      601 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
      661 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
      721 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
      781 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
      841 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
      901 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
      961 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
     1021 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
     1081 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
     1141 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
     1201 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
     1261 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
     1321 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
     1381 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
     1441 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
     1501 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
     1561 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
     1621 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
     1681 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
     1741 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
     1801 atattgaatg atggggaaga tattaagtgg attcaaaaac aaata
//
LOCUS       DAYYKC010000199         1824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000199 DAYYKC010000000
VERSION     DAYYKC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.207"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..622)
                     /locus_tag="ACHBBW_003007"
     CDS             complement(<1..622)
                     /locus_tag="ACHBBW_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154611.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKEL"
     gene            complement(633..1301)
                     /locus_tag="ACHBBW_003008"
     CDS             complement(633..1301)
                     /locus_tag="ACHBBW_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9154612.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1431..>1824)
                     /locus_tag="ACHBBW_003009"
     CDS             complement(1431..>1824)
                     /locus_tag="ACHBBW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9154613.1"
                     /translation="YLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLILFQSAG
                     TKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILK
                     HSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          661 a          300 c          238 g          625 t
ORIGIN      
        1 ttaattcttt caaatagtaa aaacgatgtt cagctataac aatagtaaat cctttatttt
       61 ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg
      121 gttcatccaa tataattaat ttagagttta gcgataaact acatccaata gaaattttct
      181 gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata
      241 attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca
      301 tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac
      361 ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt
      421 caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta
      481 actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta agactgcctt
      541 tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag
      601 aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata
      661 gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt
      721 cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc
      781 atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa
      841 aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga
      901 taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt
      961 attttttcta aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag
     1021 atcattacga ccggcaacat tcgaaataca atatataagg atgtcgcaaa aaatatagta
     1081 aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc
     1141 aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata
     1201 atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa
     1261 atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat
     1321 aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat
     1381 cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt
     1441 aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt
     1501 tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag
     1561 aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac
     1621 ttatctagac aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct
     1681 tgatatttgg ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa
     1741 atataattaa taaatggatc taattcattg aagttctctc tccaactttt ctctatatct
     1801 atattttcta agtttaagag gtat
//
LOCUS       DAYYKC010000200         1772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000200 DAYYKC010000000
VERSION     DAYYKC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.208"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..217
                     /locus_tag="ACHBBW_003010"
     CDS             <1..217
                     /locus_tag="ACHBBW_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154614.1"
                     /translation="VLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILI
                     SIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            227..883
                     /locus_tag="ACHBBW_003011"
     CDS             227..883
                     /locus_tag="ACHBBW_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9154615.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            883..>1772
                     /locus_tag="ACHBBW_003012"
     CDS             883..>1772
                     /locus_tag="ACHBBW_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154616.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGI"
BASE COUNT          747 a          185 c          307 g          533 t
ORIGIN      
        1 tgtattacta aaagtaattg tagatggatt cattaactca gaggattcaa tgatttccgg
       61 tggaagttta ataaataata gtagttttat gtcaaatata acaacacttg gacaaagtta
      121 tttaatatta ataagtatta ttgttttatc agttgtattt gcttcttcat taatgttaat
      181 caagaagcct aaagaaatct tatcaaaaat aagttaggag aaaataatgg atatattaga
      241 aataaagaat gtagcatata gttatgcaaa ttctaaagaa aaagttttgt caggagtaaa
      301 tcaaaaattt gaacttggga agttttatgc gatagtagga aagtcaggaa caggaaaatc
      361 tacacttctt tccttacttg ctggacttga taaacctcaa acaggaaaaa tattgtttaa
      421 gaatgaagat atacaaaata aaggatatag taatcataga aaaaataata tatctttagt
      481 atttcaaaac tataatttaa tagattattt atcaccaatt gaaaatatta gattagtaaa
      541 taaatcagca gatgaaagta tcttgtttga actaggttta gataaaaaac aaataaaaag
      601 aaatgttatg aaattatctg gtggacagca acaaagagta gctattgcta gagcattggt
      661 atcagatgct ccaataatac tagctgatga gcctactggt aatctagaca gtgttactgc
      721 tggagaaata attaatatat taaagacatt agctaaagat agaaataaat gtgtaatagt
      781 tgtaacacat agtaaggaag tagcagattc tgcggatatc attttagaac taagtggtaa
      841 aaagttgaaa aaagtaaata aaatgaattt ggaggttgaa taatgataaa aaatgcattt
      901 gcttatgtaa ctagaaaaag tttaaaatca ttaattatca tattagttat tttatctatg
      961 tcaactttaa gtctcattag tttatctatt aaagatgcta cggacagagc ttcaaaagaa
     1021 acatttgcta atataacaaa tagtttttct atggagataa atagacaagt aaatccagga
     1081 acacctagag gtgggggaaa tgtaaaaggt gaagatatta aaaagatatc tcaaacagat
     1141 agtatagact cctatgtaaa aagaataaac agtgttgcag atttagttga ttatgatatt
     1201 attgaaactc aagagactct tgcgaatcaa tcacctgaaa gagcaaagaa ttttaaaaga
     1261 acagttatgt taactggggt taacgactca acaaaagaaa caaaatttgt atcagaagca
     1321 tataaattag tagaaggaaa gcatttagaa aatgaagata aaaataaaat cttaatgcat
     1381 aaagatttag ctgaaaaaaa taaccttaaa gttggagata agataaaaat aaaatctaat
     1441 ttatttgatg ctgataatga gaaaggtgca gatgaaacag tagaagtaga aattaaaggt
     1501 ctattcgatg ggcataatag cggtggagta agtgcagcac aagaactata cgaaaataca
     1561 ttaatcactg atattcatac agctgctaaa gtttatggta ataccgaaga tacagctgta
     1621 taccaagatg caacattctt tgtaaaaggt gataagaatc ttgatagtgt aataaaagat
     1681 cttgggaaat tagatataaa ttggagagaa tataatttga ttaaaagttc atcaaattac
     1741 cctgcattac aacaatctat atcaggtatt ta
//
LOCUS       DAYYKC010000201         1763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000201 DAYYKC010000000
VERSION     DAYYKC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.209"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHBBW_003013"
     CDS             <1..1604
                     /locus_tag="ACHBBW_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9154617.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          640 a          283 c          357 g          483 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cgg
//
LOCUS       DAYYKC010000202         1760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000202 DAYYKC010000000
VERSION     DAYYKC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.210"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHBBW_003014"
     CDS             278..820
                     /locus_tag="ACHBBW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154618.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACHBBW_003015"
     CDS             976..1425
                     /locus_tag="ACHBBW_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9154619.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>1760)
                     /locus_tag="ACHBBW_003016"
     CDS             complement(1455..>1760)
                     /locus_tag="ACHBBW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9154620.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          655 a          256 c          334 g          515 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt
//
LOCUS       DAYYKC010000203         1720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000203 DAYYKC010000000
VERSION     DAYYKC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.211"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1533
                     /gene="guaA"
                     /locus_tag="ACHBBW_003017"
     CDS             <1..1533
                     /gene="guaA"
                     /locus_tag="ACHBBW_003017"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9154621.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT          523 a          355 c          367 g          475 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
     1681 tttattcact acctttattt tcctattgaa ttagcttttt
//
LOCUS       DAYYKC010000204         1690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000204 DAYYKC010000000
VERSION     DAYYKC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.212"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACHBBW_003018"
     CDS             complement(541..1566)
                     /locus_tag="ACHBBW_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9154622.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          526 a          269 c          326 g          569 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt
//
LOCUS       DAYYKC010000205         1683 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000205 DAYYKC010000000
VERSION     DAYYKC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.213"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1447
                     /locus_tag="ACHBBW_003019"
     CDS             <1..1447
                     /locus_tag="ACHBBW_003019"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9154623.1"
                     /translation="ADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIS
                     PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRN
                     VTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQI
                     VTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLIL
                     HGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGK
                     LAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIG
                     MPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYA
                     ERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVL
                     EKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1440..>1683
                     /locus_tag="ACHBBW_003020"
     CDS             1440..>1683
                     /locus_tag="ACHBBW_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9154624.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          590 a          281 c          357 g          455 t
ORIGIN      
        1 agcagatgaa tccttagagg aatatgatac ggtaagcaaa caaacgatgt tgtatcgcga
       61 attactttca gatgaagaat caaaagaaga tctgattgca acaatcgttg atattttggg
      121 gtatgcgatt tcgccagagt atcttttcaa cgtcttagcg gatcaagcaa aacaagcaac
      181 gttccaattg aatgatctaa acaaagcctt tgtgcaattg gcatctacct acaatcaatt
      241 taatgggtta tttgatgatg tggatctcca atcaaaaaaa ctaggaacag atgagcaaca
      301 acgaaatgtg acgattaccg aagtcattaa gaaattgaat gacgtggaag ttttaggtca
      361 cgatggggac gtgatcgggg atgcttatga atttctgatc agtcaatttg cttcagaagc
      421 agggaagaaa gccggagaat tttacacgcc tcatatggtt tctgatatga tggcacaaat
      481 cgtgacgttg gatcaaaaag aacgacgatt ctttagtgtt tttgatccaa ccatgggttc
      541 aggttcattg atgttaaatg tgcgtaacta cttaactcac ccagataacg ttaaatatca
      601 tgggcaagaa ttaaatacaa cgacatacaa tctagcaaaa atgaacttga ttttacatgg
      661 ggtagatgcc gaagaaatga atctccgcaa tggggatacg ttgaataagg attggccaac
      721 agatgaacct tacacatttg atgcagtagt aatgaaccca ccttattcgg ctaattggtc
      781 agcagatacg accttcttag acgattctcg gtttaatcgt tatggaaaat tagcgccaaa
      841 atccaaagca gattttgcgt ttcttcttca tggtttctat catttgaagg aaacagggac
      901 tatggccatc gtcttgccac acggagtttt attccgtggc gctgcagaag gggtcattcg
      961 tcaaaaatta ctagaagacg gcagtattta tgctgtgatc ggtatgcctg cgaacttgtt
     1021 cttcgggaca tcgattccaa cgacggtgat tgtattgaaa aagaaccgcc aaactcgaga
     1081 cgtattattc attgacgcta gtcgagaatt tgtcaaagga aaaaatcaga ataaattatc
     1141 ggaagaaaac attcaaaaaa tcctcgaaac ctatgctgaa agaaaagatg tcgagaaata
     1201 cgctcatttg gcaaactttg atgaaatcaa agaaaatgac tataacttaa atattccacg
     1261 ctatgtagat acgtttgaag aagaagaacc aattgatatg gttcatgtcg gaaatgacat
     1321 caagaagata cgacaagaac agcaagtgct agaaaaagaa cttttagaag ctatctcttc
     1381 cctgcaaaca acacctgaaa atgaagcgtg gttacaaggt gcgctggagg tgttcaaaca
     1441 tgagtaatga tatacaacca gaaattcgtt tcccaggatt tacggaagct tgggaacagc
     1501 gtaagtttga atgcttactt gataaaaaag atggagtaag gaggggacca tttgggagtg
     1561 ccttaaagaa agaattcttt gtatctaata gcaattttgt tgtctatgaa caacaaaatg
     1621 ctatttatga taattacgaa acaagatata aaattactga aaaaaaatat aatgaattaa
     1681 tca
//
LOCUS       DAYYKC010000206         1669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000206 DAYYKC010000000
VERSION     DAYYKC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.214"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACHBBW_003021"
     CDS             <1..613
                     /locus_tag="ACHBBW_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9154625.1"
                     /translation="TKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGM
                     KDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSG
                     MIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQ
                     FQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            1036..1669
                     /locus_tag="ACHBBW_003022"
                     /pseudo
     CDS             1036..1669
                     /locus_tag="ACHBBW_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          586 a          306 c          292 g          485 t
ORIGIN      
        1 cactaaagaa gtactgagtt atgcgattgc tccaactgaa tcaacatacg tttggaatga
       61 gctgctacag gatattaact ccagaggagt tcaagaagtc ttgcttttta ttacggacgg
      121 cttaaaaggc atgaaagata ctatccatca aatttatcct aaagcaaaat atcagcattg
      181 ttgtatccat gtatctcgta acatcgctca taaagtacgt gtcaaagacc gaaaagaaat
      241 ctgtgatgac tttaaggctg tttatcaagc taactcaaaa gaagaagcga ataccttctt
      301 atccggcatg attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt cactcataga
      361 aaaccaagac ttattaactt tttatgattt tccacctagc attcgtagaa ccatttactc
      421 aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta gaaaagagca
      481 gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata catacaatca
      541 aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat tagtttcaat
      601 gtttactgag taactaatta ttttgcagga ggacaattta tttacacaaa attattgacg
      661 ctccctgttt caaagttagt agagaaaatg tagttatata tttaaatttt taccagaaag
      721 aaatgaacac cttcaattat ctaaatctcg aaaatggtta gatgtaatca tcaggaaaaa
      781 caatcttaaa cgaattacta ttcatggtct tagacacaca catgctagct tgcttttagc
      841 aagctagtgc aaatattaaa gatgtgcaag agcgtttagg ccactcttct attcaaatca
      901 ttatggatct atatattcat attacagaca aaagaaaaga agaaaccgta gcgcaattcg
      961 caaaatatag gctctataat ttatgggact gatgaatcaa aattgattct tcagtcccat
     1021 tgttatttta ggtatatgat tcgtacattg aaagagttga aaaactatct tccgaagccg
     1081 tcaaaaagga ccctgccaaa agtattgatg gtggatgaat ttcgttcgca cgcttcaatt
     1141 gtggataaaa tgagctttat ttgtgcggat ggcgagactg gaaaactcat tgatattttg
     1201 cctacacgaa aattgtctcg attaacacac tattttagtc gctgtgcaaa tcctgaagcg
     1261 gtggaatatt tagtgacaga tatgaacgcc gcctacttcc aactgacaaa acgtgtctta
     1321 ccaaatgcga aagtcgtcat tgattgtttt catatcatca aacatttgaa tcaggcgttc
     1381 aatgattttc gtgtacaaga aatgaaaaga ttacaccagt tagggcaacg acgggaagcc
     1441 gagaaagtga aaaagaactg gcgctttctt ctaaaaaacc gtcaaaaaat caatcactat
     1501 gattacaaaa catggcgcag ttttcgtgca cctaaatacc cgctactcac tgaagcaatg
     1561 atgattgatc gattgctggc attttcaacg cctttaaagg acgcctatac ttttttccat
     1621 gagctgaccg aagcctttag agacaaggaa tcagacttat tttttacct
//
LOCUS       DAYYKC010000207         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000207 DAYYKC010000000
VERSION     DAYYKC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.215"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBBW_003023"
     CDS             131..505
                     /locus_tag="ACHBBW_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9154626.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBBW_003024"
     CDS             complement(619..1248)
                     /locus_tag="ACHBBW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9154627.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBBW_003025"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBBW_003025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYKC010000208         1599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000208 DAYYKC010000000
VERSION     DAYYKC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.216"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHBBW_003026"
     CDS             complement(<1..613)
                     /locus_tag="ACHBBW_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9154628.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..1554
                     /locus_tag="ACHBBW_003027"
     CDS             901..1554
                     /locus_tag="ACHBBW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9154629.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          575 a          223 c          297 g          504 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatacgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa
     1501 ttagactgta taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat
     1561 taaagatatt tccaaaaatg ttcatacaaa tattttctg
//
LOCUS       DAYYKC010000209         1594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000209 DAYYKC010000000
VERSION     DAYYKC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.217"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..249
                     /locus_tag="ACHBBW_003028"
     CDS             <1..249
                     /locus_tag="ACHBBW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154630.1"
                     /translation="TIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(426..554)
                     /locus_tag="ACHBBW_003029"
     CDS             complement(426..554)
                     /locus_tag="ACHBBW_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154631.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(611..850)
                     /locus_tag="ACHBBW_003030"
     CDS             complement(611..850)
                     /locus_tag="ACHBBW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154632.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1136..1450
                     /locus_tag="ACHBBW_003031"
     CDS             1136..1450
                     /locus_tag="ACHBBW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154633.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          544 a          218 c          279 g          553 t
ORIGIN      
        1 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtgatt
       61 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
      121 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
      181 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
      241 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
      301 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
      361 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
      421 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
      481 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
      541 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
      601 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
      661 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
      721 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
      781 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
      841 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
      901 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
      961 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
     1021 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
     1081 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat agcttttgga
     1141 tttttacagt aattttatct taataatagc aatattatta cttctaaata tttggttttt
     1201 tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt cttctgaaaa
     1261 aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca gtttactttc
     1321 ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta tatctattat
     1381 tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag agaagtcaaa
     1441 aaataattaa agcaattatg ccttttagta caggaattat tacttcttac aaatgtatag
     1501 cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa caattatttt
     1561 atgttaggat gaattaattt ctctagagtt tata
//
LOCUS       DAYYKC010000210         1583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000210 DAYYKC010000000
VERSION     DAYYKC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.218"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..845
                     /locus_tag="ACHBBW_003032"
     CDS             3..845
                     /locus_tag="ACHBBW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM9154634.1"
                     /translation="MKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFW
                     NSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNA
                     FQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKA
                     TNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWK
                     FVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLK
                     KIAL"
     gene            865..1425
                     /locus_tag="ACHBBW_003033"
     CDS             865..1425
                     /locus_tag="ACHBBW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFM9154635.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT          593 a          216 c          288 g          486 t
ORIGIN      
        1 taattaaaaa ggatgagatt ataatggggt tactggttcc gagagctaat acttatacta
       61 tacctttaac gaaacgccaa ttaattgcta aaagatatca aaggattact agagccatca
      121 atagagagtt ttggaattct gaaagtgata ctgctcacag tttatacgta ggttcttatg
      181 ggagaggaac ggcaattagt actagtgata tagatataat agttgaatta ccgatggcag
      241 aatttgacag attcaaaaat tatttatcaa atggcccgtc taagttatta caagttataa
      301 aaaacgcatt tcaggaaata ctaccaaata gtgatattag agctgatgga caagttgtta
      361 aaataaattt ccatgatgga atcaaatttg aaatagttcc agcttttaat gaaaaagatt
      421 attggggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg aactggaaag
      481 ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa agtaataatt
      541 tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt acgagttatc
      601 atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat tggaaattcg
      661 ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca gctttattag
      721 aatactataa ctcacataaa gttatgggcg gtttaaattt atactctcct ggaagcaatc
      781 aatttgttaa ttctgatagt agtatcatat gtttagaaaa agtattaaaa aaaatagctt
      841 tataaagttt tatggaggaa caacatggga gacgaaaaaa agcgcttgga agttcaatta
      901 cgtgaagcat atggacgagt aacgtatact tacaccactc atttaaagca agccgataat
      961 attatttctg acaataaaat tttaaggtgt cttcaaattg gtttgtcagc ccttactact
     1021 ggaggggtac ttagtaccat tattactgat aatagtgagt taacctttgt tgctggatta
     1081 tgttcaacaa tttctcttgg attaaactta tactttaaga attttgatct tgctgaagaa
     1141 gctaaaaatc atcaaattgt tgccgataaa ttatggttga tcagagaaga atacgtaagc
     1201 ttactaacag atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag agatcaatta
     1261 atgaacgaaa ctcatgaagc atataaaaat gcccctaaaa caaatagtaa aagctatgaa
     1321 tcagctcaga aagcattaaa atctgaagaa gagcaatttt ttacagaaga ggaattggat
     1381 aagatgttac cagagcatct tagaaaaaag agttctaaat attgaactct ttttttatgc
     1441 ttaaaattct cttgtaattt ttctattcat ctatatttaa gaagaaatat gatgatagaa
     1501 acatctccta aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagata
     1561 tgctcatctc accatgaacg ttt
//
LOCUS       DAYYKC010000211         1562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000211 DAYYKC010000000
VERSION     DAYYKC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.219"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACHBBW_003034"
     CDS             <1..198
                     /locus_tag="ACHBBW_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154636.1"
                     /translation="IIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFINIRGTAFV
                     NGILPLFRENIIFKAVYYFFE"
     gene            235..1173
                     /locus_tag="ACHBBW_003035"
     CDS             235..1173
                     /locus_tag="ACHBBW_003035"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM9154637.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1425..>1562
                     /locus_tag="ACHBBW_003036"
     CDS             1425..>1562
                     /locus_tag="ACHBBW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9154638.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DR"
BASE COUNT          593 a          180 c          281 g          508 t
ORIGIN      
        1 attatttatg ccagtgtatt aatgagtaat tataagagaa caaaagtaaa gaattatatc
       61 tacttgcttg cgtatttctt ttttatcatt gtgtgtttga tattcatcaa tataagagga
      121 actgcttttg taaatggaat cctaccatta tttagagaaa atattatttt taaggctgtt
      181 tactattttt ttgagtaaga ataaatagca cttaaaaata gagatgaggg atttatggga
      241 agacaagaat tatatagttt agctatcaat gaattggaaa aaagaaatct tgaaatacat
      301 gaattaaatc aaaaaaataa ttctatttta ggcatgaaaa tacgaaataa aattaatcaa
      361 cttaaccaaa tgaaaaaacg agataatctg ttgccagcaa aatataccct taaatcgatg
      421 accggtaagg ggcctattaa agactattca aacagagaga atcgcagtga tgaactaaga
      481 aaaaaagagt taactaatca aggaacacct aaaaggattg ctgtgtatag ttgtatcact
      541 ggtaattatg attctattaa agaaccggat ttttctcatc cattggtaga ttactatttg
      601 tatacagata atgtgattac aagtgagcat tggaatatta gagagatacc taaatatata
      661 tcggaattaa aaaataatat tttgatcaat agatatatta aatttcatcc taatgagcta
      721 tttactgact acgattttac tttgtatgtt gatggtaatg taacaataac ttctgatatt
      781 accggattac tgaacagttc ttccaaactt gggttaagta tgcataaaca tagtgaaaga
      841 aaatctttat attctgaggc agatgcatta attgcagttg gcaaaggtaa taaggaaagt
      901 attatagagc aaattagtaa gtacagagaa gaaggatttc ctgataattt tggattggct
      961 gaagccacgg ttattttagt tgatttaaat aattcaatcg ctgagaatct tctagatttg
     1021 tggtggagag agtatttgaa ctcagaaagt gggcgagatc agctcgcatt tccttacgtg
     1081 ttgtggaagt ccggacatgc aatgaaggaa gtgactacct taggcaataa tgtatataaa
     1141 agcgctaaat taagaatcat aagtcattat tagccctaaa tgatttctaa actattcttt
     1201 caaatcctag taaatatttg gagatagaag atttcctttt taaagtcata aaaaattaag
     1261 ttatattttt tgagtgaatg gagaagacgc atagtatcat aaacttcgtt atatcttaaa
     1321 ttatggggta tgcctaaata ataaatgtaa tacgagtata aagaagaaag caattttggg
     1381 tttaacccta ttggttcctt tcagaaaaag gtggatgaat aaaaatgaaa atattaataa
     1441 caggtggagc aggatttatt ggttttcatt tgtctagaaa attactcaag gaaactgaaa
     1501 atcaaattgt agcgattgat aatctcaatg attattatga tgtccaatta aaagaagatc
     1561 ga
//
LOCUS       DAYYKC010000212         1553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000212 DAYYKC010000000
VERSION     DAYYKC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.220"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(525..1139)
                     /locus_tag="ACHBBW_003037"
     CDS             complement(525..1139)
                     /locus_tag="ACHBBW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9154639.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          512 a          291 c          247 g          503 t
ORIGIN      
        1 tttcatttta caatcattga aaaattgaat ttcattgcaa aaaaaataat aaacacttat
       61 aaatatccat tatctatcct tcaatttttg tgtattttat ttctattaga aaaaggtatc
      121 ctgttggaca attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact
      181 ataattactt tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa
      241 gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca
      301 ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact
      361 ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa
      421 gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat
      481 gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg
      541 tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca
      601 agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt
      661 aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta
      721 tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca
      781 ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga
      841 agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct
      901 tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc
      961 ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc
     1021 aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc
     1081 tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt
     1141 tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt
     1201 acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa
     1261 aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca
     1321 tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc
     1381 gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat
     1441 tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat
     1501 ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaa
//
LOCUS       DAYYKC010000213         1525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000213 DAYYKC010000000
VERSION     DAYYKC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.221"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..322
                     /locus_tag="ACHBBW_003038"
     CDS             23..322
                     /locus_tag="ACHBBW_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9154640.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(358..729)
                     /locus_tag="ACHBBW_003039"
     CDS             complement(358..729)
                     /locus_tag="ACHBBW_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154641.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(731..1132)
                     /locus_tag="ACHBBW_003040"
     CDS             complement(731..1132)
                     /locus_tag="ACHBBW_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154642.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1146..>1525)
                     /locus_tag="ACHBBW_003041"
     CDS             complement(1146..>1525)
                     /locus_tag="ACHBBW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154643.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          489 a          264 c          212 g          560 t
ORIGIN      
        1 aaaacagatc ggagtaatgg ttatgctttt taatgagaca caattatggt ttaaatttga
       61 cccttcgaat agatttatca aagattttta taaagtatgg aattcagaag ttttcttttt
      121 agcaatcgaa gatagcttat taatcaatct ctactattct aataagaact atttcaaaat
      181 tcctgctgcg aaaactagaa tgaagaaggg tgtatacttt ttgtttgata tcgtgactga
      241 cgtgccggac gcacgtagcg atcatcggcg ttatgactat ataaagtata ctttcgttga
      301 tccagaaaga tacaaagatt aaataaaaaa ataacaaaca aactcgcttg aaattcatta
      361 ggtgagtttg tttattattt tttctgcttc tcgattcatc agcgccatat ctgttttcgt
      421 aatggtttca ttaggaatat agtcagcaca ttttcttttc tctaacattc tatacccact
      481 attatgtatt cctaaggtat tcaattcctt aactgttgaa aacaaattcc aaacggtttg
      541 gtggcttcct tttccgctac ttccattata aataatatca taattttctt tcaaatactc
      601 atcagatttt ttaaaagcac tgtagtatgc tcttgaaaca gcagttcttc tataagattc
      661 tttagaatta tatttatcat ctgattcaat ttgtttggaa atagataaat attcattaaa
      721 gtcaaacatt ttattccccc aaagttgcta ctacagattt ccccatatat ttatagtcag
      781 ataatacatt atcgagagta tccaaaacat cgtaatctga ggaaaacaat tcagatgttg
      841 ttttgataac aaagaaatac aagtttaaat cttcatcata ttttatattg atttcagctt
      901 ttctttttaa aaccgtattt atcacatcaa aaattttgcc atcattactt gaaattgtgt
      961 taatctcatt ttttgtagta attacaagtt gattatcatt tttagttttc ggagtagaac
     1021 ttgcatccac aacaactgga gatgctagaa ttaccgatgc gcaagcaaaa atcgcagcaa
     1081 aatttattaa gctaccactc cgatgtgtat ttgatggtac tatatcaagc atccgtatcg
     1141 tctccttaat ttttttccac tggtttgttg accttgatat caaagatttg ccttccaatc
     1201 tcagttccat taaatttaac aacagcataa tgctctcctg cttcttcaac caagacattt
     1261 ctggcgttta tattaaagtt tatatcgtta acttgaggag gcaagcttac cgatacttca
     1321 cccgtagaaa aaatttgttt ttcattcttg tttaaaactt caatttctac tatttttatc
     1381 ttttctgtat cctctatctc agacaccaca gctgttacag aaaatgataa agcagtagga
     1441 attaaaggcg cattaataga aagagctgga ttaattactt tattaataac attagtgcca
     1501 aattgctgac tttctacatc ttctg
//
LOCUS       DAYYKC010000214         1496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000214 DAYYKC010000000
VERSION     DAYYKC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.222"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..1374
                     /locus_tag="ACHBBW_003042"
     CDS             334..1374
                     /locus_tag="ACHBBW_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9154644.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          589 a          195 c          232 g          480 t
ORIGIN      
        1 tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa tttatttttt
       61 gttttgacac tggtagtact taattatctt taattttaaa actaaatttt attcatacgt
      121 ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta atgactttaa
      181 aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa aaaacagttt
      241 taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg taattttaca
      301 taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt ttttaaggcg
      361 gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt ttctgacgaa
      421 tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc aagattagag
      481 atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac ttatacgaca
      541 aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat caaaaaagag
      601 ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca gttagggaaa
      661 aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga tatctactta
      721 ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg tatgaaaatc
      781 atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga gggtatgaaa
      841 atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc agaacttaat
      901 aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg cctacaagat
      961 caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc tacgtttgtc
     1021 cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga agctcaaaaa
     1081 accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtga tatttcgata
     1141 gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata cacgacactg
     1201 ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt
     1261 gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa
     1321 cgtttgaatt atgcaagctc cccaagcact attacgaaag actggttaga gtgaaacttt
     1381 actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt tttattattc
     1441 taattatatt acgaatatta caaaataata gctattttaa tagaatttat aagtta
//
LOCUS       DAYYKC010000215         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000215 DAYYKC010000000
VERSION     DAYYKC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.223"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHBBW_003043"
     CDS             64..972
                     /locus_tag="ACHBBW_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9154645.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHBBW_003044"
     CDS             972..>1480
                     /locus_tag="ACHBBW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154646.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYKC010000216         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000216 DAYYKC010000000
VERSION     DAYYKC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.224"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHBBW_003045"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHBBW_003045"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9154647.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          497 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta ta
//
LOCUS       DAYYKC010000217         1394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000217 DAYYKC010000000
VERSION     DAYYKC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.225"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1392
                     /locus_tag="ACHBBW_003046"
     CDS             join(53..494,494..1392)
                     /locus_tag="ACHBBW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9154648.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          583 a          182 c          243 g          386 t
ORIGIN      
        1 gatgtgcacc ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa
       61 atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta
      121 caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat
      181 ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt
      241 taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa
      301 aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg
      361 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
      421 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
      481 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
      541 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
      601 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
      661 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
      721 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
      781 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
      841 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
      901 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
      961 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
     1021 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
     1081 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
     1141 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
     1201 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
     1261 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
     1321 aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag
     1381 tccattaaat agtt
//
LOCUS       DAYYKC010000218         1392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000218 DAYYKC010000000
VERSION     DAYYKC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.226"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..417
                     /locus_tag="ACHBBW_003047"
     CDS             7..417
                     /locus_tag="ACHBBW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9154649.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            431..787
                     /locus_tag="ACHBBW_003048"
     CDS             431..787
                     /locus_tag="ACHBBW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9154650.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          207 c          260 g          414 t
ORIGIN      
        1 gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa agcaagagca
       61 tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga accgttaaca
      121 gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga tattatttcc
      181 actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac tttaaagcaa
      241 atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt gattgatgaa
      301 aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc tcgtgaaatt
      361 cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa ttcataggga
      421 gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc
      481 gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag aagaacgcga
      541 tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag cagaacagct
      601 atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct gtgtaggaaa
      661 aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc ctttgattat
      721 tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc ttatcttaaa
      781 taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt gtaacagtcg
      841 aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc tactatagag
      901 acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag ttaaaaatat
      961 attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta gcaaagcaat
     1021 aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat gacatgataa
     1081 gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga aaaaaacttc
     1141 aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc tgtaagattt
     1201 cgaaaaacga aataaaaaaa acttttaaaa aagagttgac aattacttgt ctctctgata
     1261 tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg atgaagtaga
     1321 cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg attcaattca
     1381 agacaacaaa ca
//
LOCUS       DAYYKC010000219         1391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000219 DAYYKC010000000
VERSION     DAYYKC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.227"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..1145)
                     /locus_tag="ACHBBW_003049"
     CDS             complement(222..1145)
                     /locus_tag="ACHBBW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154651.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          450 a          247 c          140 g          554 t
ORIGIN      
        1 ttgtaaatgc ttctgaattg aagtgtgatt cattttcatt aattaccact cactttctaa
       61 aaaattttta tttgtatgac tacattacct ccaacctctc ataatttcat gatcttcagt
      121 tctacaaaaa tatatcccac tcaaaatctt gacaaaagaa aaaagttgtg attttggaaa
      181 tcacaacttt tttcttttac gataaatcaa tccttattaa attatttagg aaaatattgt
      241 tcaattattt ctttatatat tttctcatag tatgtttctg tccatcctaa tgttcctata
      301 aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt aataatgttg
      361 tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc aatttcctcc
      421 caataatatg aatctctttc aattcccatg atggtagctt tggctaattt caaatcaaat
      481 aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc tttaatccat
      541 tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt atatttcaaa
      601 tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct tttaagtact
      661 tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt attcactaat
      721 aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg aaaatcacct
      781 ttataaactc cattcttttc cactgtttta ttaatatttt ttaaaaacca gaatgagttt
      841 gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg taataataag
      901 acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc aatcataggg
      961 agccattttt tgattttcac taattgattg ctcataaatc tttcactgga cttttgttca
     1021 atcttaaatt gcaaaacttc taactcataa ttcccatctt ctgatttatt tttgggaaca
     1081 actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc ataatttgta
     1141 gccattaata catctcctta ttcataataa atttcatatg acgctcctct caaaatttac
     1201 cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct taaaaaaaaa
     1261 ctggtctctc taccggtaat atcgattgag tccgccttta tcgactttat ccttttttta
     1321 tattaaattt tttcaccggt agacagggag agaaagaata aactaaaaag cccttaattc
     1381 ttaatgtttt c
//
LOCUS       DAYYKC010000220         1389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000220 DAYYKC010000000
VERSION     DAYYKC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.228"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1389
                     /locus_tag="ACHBBW_003050"
     CDS             <1..>1389
                     /locus_tag="ACHBBW_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154652.1"
                     /translation="NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSA
                     IAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMII
                     VRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLE
                     ALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVT
                     NLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVF
                     LLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEK
                     HEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKK
                     HGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYV
                     TKKEINKKNENK"
BASE COUNT          487 a          202 c          246 g          454 t
ORIGIN      
        1 taaatatttg tacgattctg attagttttt attatggaga aaagtttttt aaaaagatag
       61 atgctgcttt tttatttagt tgtttatata cttttgcaac ttatcgaaca gttgatatct
      121 attatcgatc cgcaattgca gaagcaatcg ctcttacgat tatcccagct gttttatatt
      181 atgcttatgg attgatattc aatagagaaa aaaaagtcat cgctttatct gtcagtataa
      241 gtctacttgt gtatactcat gtcttaacta cactattgtg tagtgcattg attgcttgta
      301 tgattatagt tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa tttgtggtcg
      361 tgaatttagt aaaggctgtt tttttgactt gtgcattaac tagctatttt tggtatccta
      421 tgttgcagca gatgaattat caagaaatac acagtccctt caaaacagat ttgcagctag
      481 aagcactttc tatttcagat agtatcattc aagcgttaaa taacgatatt acaacataca
      541 ctatggggtt attaggattg gttgcgttga tttttccttt attcatactg aaagaaatgg
      601 agaaaaaaga aaaaatcatc tatgttggcg tagtttctac atgggtatgc gtaactaatt
      661 tatttccatg gtttttgttt caaaaaacac cgataagctt actccagttt ccttggcgta
      721 tcttaggtat ccaaatcttt ttttcttcaa tgatcataac gcttgttttt atgaaaaaga
      781 ctatgaataa aaaatattca tggttaatca ttggcgctac tgtggtgttt ctactcgtca
      841 cgtctatagc agcaaaagaa aattacgcaa aagttatcca atcaaaacac ggtcatattg
      901 ttatcaatga agaaacactc ccacgttata caacatcaga tatgggagga ttatacgatt
      961 acgccccaac agaagcgata aaagaaaggc ctcacttaga aaaacatgaa gtaaaagtag
     1021 gagaatcgtg ggaaaaaggg aagtataaag ctgcagataa taacattact tacacagtgg
     1081 caagcaacaa aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt acacaggtaa
     1141 aagtaaacgg taaagtcgta aatgactctt ataaaaaaca tggcttagtg aatgtggatg
     1201 taaatgctgg agaaaataag atagaaatta caacaaaata tactccaact gctctcatgg
     1261 ctttatggat cagtattctg acttattgtt tagtggtgta ttcaagtttt aaacgatttg
     1321 cacctaataa gttgaaagat tctcgatatg tgactaaaaa ggagataaac aagaagaatg
     1381 aaaataaat
//
LOCUS       DAYYKC010000221         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000221 DAYYKC010000000
VERSION     DAYYKC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.229"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHBBW_003051"
     CDS             82..>1362
                     /locus_tag="ACHBBW_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9154653.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYKC010000222         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000222 DAYYKC010000000
VERSION     DAYYKC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.230"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHBBW_003052"
     rRNA            <1..1355
                     /locus_tag="ACHBBW_003052"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYKC010000223         1339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000223 DAYYKC010000000
VERSION     DAYYKC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.231"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..394)
                     /locus_tag="ACHBBW_003053"
     CDS             complement(95..394)
                     /locus_tag="ACHBBW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9154654.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            745..>1339
                     /locus_tag="ACHBBW_003054"
     CDS             745..>1339
                     /locus_tag="ACHBBW_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9154655.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQAL"
BASE COUNT          447 a          220 c          191 g          481 t
ORIGIN      
        1 ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta
       61 tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg
      121 ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat
      181 aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt
      241 ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta
      301 tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta
      361 gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt
      421 cttcccacta cattctataa acaaagtata cgctacatct agattttttt aaatcattac
      481 tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc
      541 taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt
      601 gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa
      661 ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact
      721 ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat
      781 aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca
      841 caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc
      901 aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc
      961 gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt
     1021 gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc
     1081 gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt
     1141 gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt
     1201 tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta
     1261 agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt
     1321 aatcagttgc aagcacttg
//
LOCUS       DAYYKC010000224         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000224 DAYYKC010000000
VERSION     DAYYKC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.232"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..>1311
                     /locus_tag="ACHBBW_003055"
     CDS             54..>1311
                     /locus_tag="ACHBBW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154656.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKL"
BASE COUNT          534 a          180 c          194 g          403 t
ORIGIN      
        1 tttaattttt ttttatggta aacttgtcat acaaataaaa aaaggagatt tatatgttta
       61 tattagatga tgaaataaaa aaagttaaaa atcctagaat tattccatta ttagaagaag
      121 ttagaagtag tttttatcag ggaaattatc gttctgcaat agctgtcaat tacagtgcgc
      181 ttattttaga tttaattgat aaattatctg atttagcaaa tatttacgag gataatactt
      241 ctaagaaaat tttagaagat atagctagac taagaaaaag tgatccaaac tctccaaaat
      301 gggaaatgga actactagaa aaagcttata gttcaactga gctaattgat agctatgaat
      361 atgaggattt aaaatatatt aaggatcaaa gaaattattc agctcatcct gtagtcactt
      421 acaacggtaa tacttgggaa ttaagagata taacaaaaga aacttgtcag gatgttatac
      481 ggaagtccta tgaaattata ttcctaaaaa atccaatatt aggaaaaaaa gtaacgatcg
      541 aaattataca atatgcaagt cgcatacatg ctatgtctgt tacacctgaa gaatttcact
      601 ctgcactaaa aaatagctat ctaaataaac taagcgaaaa agccaaagaa aacctatgta
      661 agacagcctt taagtttgtt tttcaattat cttatggaaa tgaggaagca gatagaaata
      721 gggaaagtac tttcagacta cttaatgccc taatttttga taataaagaa ttctatttaa
      781 atattctaaa aaaagaaatt gcaaagtata ggttcacagc tgaatcaaga gaggaaatta
      841 ataaatcact tggtgaaaat caacagatca cgtatacaaa agggttcttt ttacttactt
      901 ttatagctac ccatccagaa ttacaacatg atttttcaga cgagacaatt caaaatttac
      961 aaatatcaat aaatgatttt aaaccgaaac aacaagtact aacagaaata gaatattact
     1021 accgtttacg ttcccttgca gttctaggaa acaacaatga agaattacaa aatcacttaa
     1081 atagtctagt tgatcctata ctccagcaaa aaatgagagt ttcttcgcta ccatcttcaa
     1141 cattagaaac aatatataat caatatttat attttggtga aaaagagatg ttcatagatt
     1201 ttatgatcga acatcttacc gatgctagtt cattcaggtt agcagataag gatatggaag
     1261 tattaccatg gatttacaat aaattaaatc aggagcaatt taacaaatta c
//
LOCUS       DAYYKC010000225         1304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000225 DAYYKC010000000
VERSION     DAYYKC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.233"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..119
                     /locus_tag="ACHBBW_003056"
     CDS             <1..119
                     /locus_tag="ACHBBW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9154657.1"
                     /translation="LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            124..1071
                     /locus_tag="ACHBBW_003057"
     CDS             124..1071
                     /locus_tag="ACHBBW_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9154658.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          424 a          187 c          274 g          419 t
ORIGIN      
        1 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
       61 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
      121 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
      181 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
      241 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
      301 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
      361 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
      421 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
      481 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
      541 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
      601 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
      661 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
      721 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
      781 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
      841 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
      901 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
      961 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     1021 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     1081 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     1141 atcaaatctg ggatagaagg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa
     1201 taatttggac agaacagaaa gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat
     1261 gttttgccat gacgaatcat aattcgtagt ttgaaattgt aaaa
//
LOCUS       DAYYKC010000226         1256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000226 DAYYKC010000000
VERSION     DAYYKC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.234"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..629)
                     /locus_tag="ACHBBW_003058"
     CDS             complement(<1..629)
                     /locus_tag="ACHBBW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9154659.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQD"
     gene            complement(619..>1256)
                     /locus_tag="ACHBBW_003059"
     CDS             complement(619..>1256)
                     /locus_tag="ACHBBW_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9154660.1"
                     /translation="TMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKE
                     IFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSR
                     WMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNIS
                     ASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT          394 a          235 c          150 g          477 t
ORIGIN      
        1 tgatcctgat gttctaaatt accatctaat tttcgataag cctcttctaa tttagaatct
       61 ataaataatt ctctgtccaa cgatttcaaa tcatatttat ccttattaat ataaaaagaa
      121 gcatctccag aaccactcaa tttaataagc ggttttgaag aatacttctc tgggttataa
      181 atttcaaaag tttctgtttg tcgaatagta aatctaccat tgtaattttc cttaaatggt
      241 cgaatactca ctgtaaattc aaaagggaca atatttcctg tttttcttgt tcctttaaat
      301 tcaggtcctt ctattactac agcttgataa atatattgtt catcatagta aagaataaaa
      361 tcactataag acgacatatc taaccattca gtgattcgat cttcccactc ctgcaccata
      421 tcaattgaag gtgctttata gtaacactca atctttctag tcacattttt gtaatagcct
      481 ttatcaataa tgatagaatc atttccttct cgctctctca attcaattac tcgactagct
      541 gaaacagaag caggcctgtt ttgaatatac acattaaatt cagaagaata atgttgatta
      601 ataaaaaatt gtcctctctt aagttgcata taatgttcct cctactgcac cagcatctcg
      661 tttcatttgt cttgtcaatt ctgttttaat ttttgtagta atctttcgaa ccattgaatc
      721 aggtaaatct ccataaacat ttaaatgtaa atgaatttct tttgtttcac tagcagatat
      781 attttgcttt gtttgtgtaa ttggtaggtg acttattcca tttactacag gttgaacaga
      841 cggtgttttt actaaatttg tcatggtttg atctaactta ctttgctctt tatcaatacc
      901 aacgataata ccttgaacaa tatttttacc aaccatatca cgcatccatc ttgatggaga
      961 atgaatatct aaagctcctt taatccaatt tttgatatta cctgcaatac ctttgatagt
     1021 atctttcaaa gcattccact tttcttttac accatttata agtccatcaa tgatgttctt
     1081 gccaatttca aataaattga cttcccttaa cgaattaaag atttctttta cccgattaat
     1141 agcatttgaa acaccatttt ttagatttgt ccatgcattt tcagcagaat taacaattcc
     1201 agtaacaata ttccagaaag agtccttaat gttattccat gtattaatca tggtat
//
LOCUS       DAYYKC010000227         1255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000227 DAYYKC010000000
VERSION     DAYYKC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.235"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..212
                     /locus_tag="ACHBBW_003060"
                     /pseudo
     CDS             18..212
                     /locus_tag="ACHBBW_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(400..1176)
                     /locus_tag="ACHBBW_003061"
     CDS             complement(400..1176)
                     /locus_tag="ACHBBW_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9154661.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT          405 a          227 c          183 g          440 t
ORIGIN      
        1 aagtgataac tacttaagat gaagcagaat atttacgcca tacggcatac aaccgcgaaa
       61 aatataaaaa aaagaaaacg attgaacgcg tttttgcagt cgcaaaggaa aagcatggaa
      121 tgcgctacat caagtatcga gacttggaaa acaatcaggt gtatacgctg attgtttttg
      181 ctgcaatgaa tctgaaaaag ttgtccactt ggcagttgga acatatgtct tcatatcttt
      241 tattatacaa aaatagcaaa agcaccagtc tacactctta gtaaagagga aactgatcct
      301 tttgtctaca ttcttaaagt acttaaacta agtactttat tttactcttt aggattgata
      361 cagtagcatc tatcccttta ttaataatct tttatttttt taaataaaga tgttgtctcc
      421 atcttcaggc actaaaactt tttcggaaat tcctttttct ttattgaaag actttaattc
      481 ttctcttgac agtccccaat ggtttacagc ttccatatga acggcaataa ttgtggaatc
      541 tggtattgct ttatgcaatt catataaatc gtctttcccc ataataagag attctccaac
      601 taaaaaatga ttatctccag catttgctac tatgatttct ggattatact gatcaatagt
      661 ttgtttaaca gcttcatacc aaactgtatc tcctgtaaag tatagtgtct tctcagtcga
      721 atgtttgaaa acaataccgc aaacattacc tacataattc aagatttctc ctctgccatg
      781 tttgcctttt gttttaacca attgtataga ttcaaaaact gtgtcttctc gtagtacctc
      841 cacattttta aaaccatata gtcgaatttc attcgcatca ttttcatctt gcacaaataa
      901 ttttatttct ttaggtagga cctctttcgc tatatcgtcc caatgatcta gatgtaaatg
      961 tgttacaata acagcatcaa taccgttgat aatatcttcg actggcattg gcaaatcaac
     1021 tagaggattc atttgatctt ttcttggtga tggaaaagga cctttcgaaa taaatggtgg
     1081 aagtgttcct ttttccgcta acattggatc aattaaaaat tttttacctc catagtctaa
     1141 gatagctgtt gcattacgaa tgtgatgtat attcataaac gttgtctcct ttttgttgat
     1201 atgaaatatt tttagtataa ttcaattcca tacaataaat aaaccgaaca aaaaa
//
LOCUS       DAYYKC010000228         1255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000228 DAYYKC010000000
VERSION     DAYYKC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.236"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1012
                     /gene="scrK"
                     /locus_tag="ACHBBW_003062"
     CDS             140..1012
                     /gene="scrK"
                     /locus_tag="ACHBBW_003062"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9154662.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          386 a          225 c          288 g          356 t
ORIGIN      
        1 attttaaaaa atgataaaaa ttcaaaaaga aaaaatagta gttatttact tgtcatgtta
       61 ctaaataaaa aaataattta aatatgatat atagtcattt atttagtgat ttcattctaa
      121 aataaaggga gaaatgtcta tgctatacgg tgcaattgaa gcaggcggca caaaatttgt
      181 gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga gtgagctttc cgacaacgac
      241 acctgaggaa acaatgcctt tagtcattga cttcttcaaa caataccaag cagatttagc
      301 agggattggg attggctcat ttgggcctat cgatatccat cgcgattccg ccacttatgg
      361 gtacattacc tcgaccccga aattggcatg gcaaaatttt gatttcatcg gtaccatgaa
      421 aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg cctatggtga
      481 gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga tcggtacggg
      541 tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc atccagaaat
      601 ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct gtccatttca
      661 tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaccgct tagggaaaaa
      721 aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct atattgctca
      781 atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg gcggtggtgt
      841 gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg
      901 gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag aagacaacgc
      961 tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat aaacgaaaaa
     1021 aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta gtcataacca
     1081 gttaggcaga gtgcttaact ggtttttgtt ttacttggaa gatcaagcct tgaatgagat
     1141 aaattcttgc tcgaaagtta tggaaatttc gatagccgta ggccacttgt attgtgcaat
     1201 agttttttac aaaaaaaagg gggtgcggat atttttttgg acctttttaa caaat
//
LOCUS       DAYYKC010000229         1249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000229 DAYYKC010000000
VERSION     DAYYKC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.237"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(384..470)
                     /locus_tag="ACHBBW_003063"
                     /pseudo
     CDS             complement(384..470)
                     /locus_tag="ACHBBW_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          435 a          175 c          163 g          476 t
ORIGIN      
        1 gcttttgtta gaaaaatcaa tacataaatt atcatatctg tatatgtcat ttaggtattt
       61 cagttatgtt ataatagttt tgctgtctgc taccccggcc taatctggca aggggaggag
      121 gagatataat gaaatacctt tttgattttg tttttgcaat caagccactt gcagatgtag
      181 tgtgcagtta cctctgcaaa tggcttgatt gcaaaattgc caaaaaagac agtaagccta
      241 cccgttaatt ctataatata gaaacgtaaa aaaaacccca tcagcctgca cgctgatggg
      301 gtttggtgat ataatgaaat acctctttgt tactcataat tataccataa ttagtcacga
      361 atgcaaccaa tagaaaaaac tacttagaac ggtagatcat cgctaataac aatgcattgt
      421 ccaccaaatg gattttgtga agcattaaat acattgttat taattgtcgc tgcttttaac
      481 taggcctctc cccttctatt ataaaagaag atatatatca tttcttaatt acctactacc
      541 ttaaaccaaa atttaagtac tacctaattt aagtagtact taaacaatct aaaaaatagt
      601 tatcccaatt gttatgcttt tataatacga ttatttgtaa caaaatgaat attatataat
      661 taaacactac catatttagg tagtgtttaa ttattagcaa aacaaaaaat atttattcta
      721 ttctcttaac ttcaactaag ttatgctgca cacctttata taaatcatat gcaactcatt
      781 agctagacaa actacaagca tgtagtttta tctgttaatt atgtactacc taaataaagt
      841 agtttataat tagcaagtat atatatttaa aaatttaaga ttaatttttt tatgacttgt
      901 acaaatttat ctgatattct cttattaaga actacctaat ttgggtagtt cttatttttt
      961 acattttaag tagtatttaa ttgcaatttg gtagtatttt tttattatct tattcttaga
     1021 acggtagtac ttaatataaa tataggtagt atctaattta agtattaatg gtagtatcta
     1081 aattaacagg tagtatgtaa ctactttaaa gtaatttttt tcagattaac acagtagtat
     1141 cttattaaat aagttaattt taatggtagt acataattat tatatatatc tgacattggt
     1201 agtacttatt gtatttttta gtagtatata atctattgtt ttctagcct
//
LOCUS       DAYYKC010000230         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000230 DAYYKC010000000
VERSION     DAYYKC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.238"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..788
                     /locus_tag="ACHBBW_003064"
     CDS             <1..788
                     /locus_tag="ACHBBW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9154663.1"
                     /translation="ISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELH
                     YSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQK
                     ANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYK
                     IGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQR
                     KEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            860..>1215
                     /locus_tag="ACHBBW_003065"
     CDS             860..>1215
                     /locus_tag="ACHBBW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9154664.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLD"
BASE COUNT          533 a          140 c          199 g          343 t
ORIGIN      
        1 ctatttctaa tatagactcg actagggaaa aaatcataaa aatatttgat aaaattaata
       61 atttattcaa gaatgacaat gctgaattaa ggaagggtct tttagagttt aaacttcaag
      121 aactagaact tcattactct tatttagtga aaattaccga agaaaaagaa caacaaaaag
      181 caataaaaga acagatgata gaagaggaaa aagttcgtag agaaattgat cgtgaaaaga
      241 aacgtattga taaagaacaa cgtcaattca attctgaaat ttccaaatta atcgcatata
      301 tgcaaaaagc taatgcagat gtggaaaaag aattatatgc aaataaaatt caagaactgg
      361 aagaaaaatt aaaagaatta gaagttatac gtgaaaatgt attacaaaga gaattaaata
      421 ctagagccgg ttatgtgtat gtaattagta atattgggtc ttttggtgaa gacatatata
      481 aaattggtat gactagaaga ttagaaccta tggatagaat aaaagaattg agtagtgcat
      541 cggttccatt tgaatttgac gttcatgcga tgattttttc agaggacgct ccgtctttgg
      601 aaactaaatt gcataatcat tttagaaaac aagaagttaa taaaattaat caaagaaaag
      661 aatttttcaa agtttcacta gacgaaatag agaaagttgt tttagaaaat tataatggaa
      721 cagtaacatt tacaaaatta gctaaagctg aacaatatag acgatcttta gaattgtcaa
      781 aagattaaat atataaaaag ccttcgggct ttttctttta acaattaata ggaacgtata
      841 ttcgaaagga atagaaataa tgtggttaga aaaaataaat gacaaaaaat ttaagtacac
      901 cgaacgttat acggatccct taactgaaaa aaagagaaaa gtgtccgtta ctttatctag
      961 caattcacgc caagcttgga atcaagctaa cttacttttg aatgaaaaaa tcgcagaaaa
     1021 aataaaacga aatgaagaat taccactaac ttttggagaa ttaaaaacta aatgggatga
     1081 aaaatacaag ccaactgtaa aagaaagttc ctatcgaaca actcaggttt atctatcatt
     1141 aatttcaaaa tatataaaag atgatgtact tgtaaaaaac gttaatagta atcttataca
     1201 agatatgcta gatta
//
LOCUS       DAYYKC010000231         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000231 DAYYKC010000000
VERSION     DAYYKC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.239"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHBBW_003066"
     CDS             414..947
                     /locus_tag="ACHBBW_003066"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9154665.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYKC010000232         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000232 DAYYKC010000000
VERSION     DAYYKC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.240"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHBBW_003067"
     CDS             <1..1076
                     /locus_tag="ACHBBW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9154666.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYKC010000233         1180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000233 DAYYKC010000000
VERSION     DAYYKC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.241"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..785
                     /locus_tag="ACHBBW_003068"
     CDS             <1..785
                     /locus_tag="ACHBBW_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9154667.1"
                     /translation="VQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPV
                     KIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVIL
                     QHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNE
                     LKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIF
                     ILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            787..>1180
                     /locus_tag="ACHBBW_003069"
     CDS             787..>1180
                     /locus_tag="ACHBBW_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154668.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKS"
BASE COUNT          394 a          141 c          200 g          445 t
ORIGIN      
        1 tcgtacaaat attttatatt acactttctt cgtctagagg tgcccaggta gtaatttgtt
       61 cttttttagt attatttctt ttgttgtttg ccaattcaaa atttagaaag agcattagag
      121 aagtactacc tgtaaaaatc aaatggatgg taagcatatt attagttcta ttggtatatt
      181 tttcttttgg tactaattat gctaaggatt ttttagcaac aattcctgta tcgattgata
      241 gaaaagtaga agttatagag tcaaatgaat cagataaaac tccacttcca gaaaaagaag
      301 taatattaca gcattctagt gataactccg aaatttcagc tggtcgtttt acgatttgga
      361 aagctgcgct taaagttata agaccaactt tgttatttgg ttatggagag accgatttct
      421 atgggaatag cgatgcaaaa tttttgaaaa aagtagatct tgatactaca gatgtgaatg
      481 aactaaaaag agcacatgga aacatgcata atggatttct tcaagtactt gtatcaagtg
      541 gtataattgc ttttatatgt atctatgctt tctatgtgtt aaatatcttt agtttactca
      601 aaacattttt tagaagtaaa gtgaattatg cgtttcttgg tattgtttta atttttatac
      661 tatctatttt tatagatgaa atggtggagg ctcatgtact tttcaataag cgcgatgtca
      721 tttcaattgt tttctggtat tatttgggaa gcatttctta cttgtattta aaaaaggaga
      781 attaatatga agacagcttt cattgcttgg actcctttac atttgattaa tatattaaat
      841 gtttatcaaa atttttaccg agaatcaaaa ggagatttat ttatttattc tgaatttgaa
      901 aacgctagaa gatatagtga gcgtataaaa gaattagggc tatttaatga tgttgttttt
      961 gtgaatcacc ggaaaatcgg ttcttccttt aaacgtaaaa ttaacttgtt aactaaccat
     1021 aatcaatttt tctcggatga tttatccgtt tatgatgaaa tttttataca gggggaaaat
     1081 tatttcacaa aaatactttt ctccaatgtt tacaaaaaaa atccagatgt aaaactaaat
     1141 tatatagaag atggtttggg tacttatttg aagaagagta
//
LOCUS       DAYYKC010000234         1075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000234 DAYYKC010000000
VERSION     DAYYKC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.242"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..431
                     /locus_tag="ACHBBW_003070"
     CDS             <1..431
                     /locus_tag="ACHBBW_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154669.1"
                     /translation="DENGQFFVWMRHEHCWCNPFVLKKENEIRTKIANGEWDYLFRWE
                     DESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQNIDIDAIECLKEVDQKYEEEPYY
                     LLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDRKSSI"
     gene            606..941
                     /locus_tag="ACHBBW_003071"
     CDS             606..941
                     /locus_tag="ACHBBW_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154670.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          391 a          129 c          219 g          336 t
ORIGIN      
        1 tagatgagaa tggacaattt tttgtttgga tgcgacatga gcattgttgg tgtaatcctt
       61 ttgtgttaaa aaaagaaaat gaaatcagaa ctaaaattgc aaatggtgag tgggattacc
      121 tcttcagatg ggaagatgaa tcttttatag caaatgatat ggatcatgca tttgtctcat
      181 ttctggataa tacatggggt acttggtacg attgttacct agaaaaattg ggctttcaaa
      241 atattgatat tgatgctatt gaatgtctta aagaagtcga tcaaaagtat gaagaagaac
      301 cctattatct cttgcaacaa aaattagcca ataattcagt gtttcaggta gaaatttggg
      361 aaaatgaaga tggaacttat gaggggctag cgcaagaaat acccttctca gataggaaga
      421 gttcgatttg aaaagagtat atcagcaatt tttatgaagt gaaagcaagc ttacaatata
      481 gtaatggaaa attgtggtta gctggaaggt atattccagc taaccaaatc ttacgttttc
      541 tgggagagtg gcgttttaaa acaatttcct aggaaaaata gttaacataa aaggaatgaa
      601 ttgttatgaa acaatattta aatgaatgga aagcgataga ggggtcatta gtaacgcaaa
      661 gaataaagga attacctgat tgcttagaga aggatcattt gtttcaaata cgagaaatgt
      721 tgaaaaaaga acagtttgat ccagaccaat ttttagtagt cgagtatcca actattggag
      781 tgtactgttg taaccatatc aatgatggaa aatattttat tattcaggag tatggaggac
      841 agttaactcc tttctatact acatgggaaa tgagtgaaga aggaatcaat aattttccat
      901 gtgaaagcat cgaagattca atttcacaca ccgaatgtta attgattata actataaaaa
      961 aggattgaac attatgttca atcctttttt caatgaaatg ttttccgcca tttcattgtt
     1021 tggtgcgatt tctattttat tatatcaaaa taaagagaga ttctaaagaa agaag
//
LOCUS       DAYYKC010000235         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000235 DAYYKC010000000
VERSION     DAYYKC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.243"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHBBW_003072"
     CDS             <1..969
                     /locus_tag="ACHBBW_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9154671.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAYYKC010000236         1063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000236 DAYYKC010000000
VERSION     DAYYKC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.244"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1062
                     /locus_tag="ACHBBW_003073"
     CDS             52..1062
                     /locus_tag="ACHBBW_003073"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9154672.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          443 a          170 c          186 g          264 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaa
//
LOCUS       DAYYKC010000237         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000237 DAYYKC010000000
VERSION     DAYYKC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.245"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..551
                     /locus_tag="ACHBBW_003074"
     CDS             <1..551
                     /locus_tag="ACHBBW_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9154673.1"
                     /translation="TEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFERW
                     RQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVRHASI
                     SERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGA
                     LIIATVDDPKQNVFTLEKVGVI"
     gene            551..>1062
                     /locus_tag="ACHBBW_003075"
     CDS             551..>1062
                     /locus_tag="ACHBBW_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154674.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          360 a          156 c          264 g          282 t
ORIGIN      
        1 aaacagaagg agaaatgggt tcttattggc aatcaatcga agatttaatt gcggatatga
       61 cggactttat taatgaaaat aaaggcgatt ttacggactg gatgaatgaa aggaaagaag
      121 aatttgaacg ttggagacaa gaacaggaac acagtttcca agattggaga gaaggacaag
      181 aatcagacta tttggcatgg tttgaatcaa tcaaggatat tttaaagacg attgatccag
      241 gtggagtcat gttagccgaa ctaacggatg cgcgagtaga tattcaagga gttcgtcatg
      301 cgtctatttc agagcgtttg ctggcagata tggactattt atatcagaaa ttgcgagcaa
      361 cgcttttcac gattgaatac ggggagattg aagtgactga tattttgcag gatgatctct
      421 tttcagataa tcatgaagtc gaaaaagttg gaactgtagc attcccgatt gaagaaggag
      481 ccttgatcat cgcaaccgtt gatgatccaa agcaaaatgt gttcactctt gagaaagttg
      541 gggtgatcta atggctaaaa ctaaacgaat gatggaaacg gatgaaaaaa cgggtgtaca
      601 acgccaattc tttccaatca cacatgcttc cgcagttctt gggttagaag aaataatagc
      661 aggagaagca acagttttat ctgttaatgg aaaaattgga gccgtcgtca ttactaaaga
      721 agatttggga ttagagaatg ttctcacaga attaccctat gcaagtgaag aagatgacgg
      781 cattatcacg gcagaaatgt atcaaaaaat tttaaacagt ggagaaggtg actacgtgtt
      841 gccagttgcc actatcgacc gtttgggtgg cataaaagtt ggtgaattat tgacgattga
      901 tgagacaggg aaagtctctg cagtcagaca atctgatgtc aatttttcgc tggagttaaa
      961 agaaaaactc gattcactga aaaattatac tgctggagaa aacatcacta ttgatgaaga
     1021 tggaacaatt agttctacag gtggtagtgg gactggtggt gt
//
LOCUS       DAYYKC010000238         1021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000238 DAYYKC010000000
VERSION     DAYYKC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.246"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACHBBW_003076"
     CDS             2..460
                     /locus_tag="ACHBBW_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9154675.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACHBBW_003077"
     CDS             469..696
                     /locus_tag="ACHBBW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154676.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DAYYKC010000239         1015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000239 DAYYKC010000000
VERSION     DAYYKC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.247"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..981
                     /locus_tag="ACHBBW_003078"
     CDS             172..981
                     /locus_tag="ACHBBW_003078"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9154677.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          337 a          176 c          197 g          305 t
ORIGIN      
        1 tttttttaac aaaaaaagcg aaaataatac ttaacgttcg tgtttagctt atgtttagct
       61 atttatatag ccataactgt agtttttgtt aggaatagct gattgctctt gttaattacc
      121 taaaatatgc tagactaaaa caaatgctag ttgaggaagg aaggaccctt attgaataaa
      181 aaattaattg ccatcgatct ggacggaact accctcaatc aagattcgat gattacatct
      241 aaaacaaaag agactctaaa aaaagcaatc aatgccggtc atcatgtcag tattgctaca
      301 ggacgcccat tccgaatgag ccatcagttt taccaacaat tagaattaac aacaccgatg
      361 gttaatttta acggcgcatt agtacacatc cctaatcagc actgggatgg tgaaagagaa
      421 acgttgatca atcgtgaaat cgcttttgaa atcttatcgc aaaaaaagca gctaaattta
      481 gattttatcg ctgctgaaaa tcgtgatact ttttttattg acagttttga tttctttgat
      541 gaaaaaattt ttgcttcttc tcgtccagga gagaagaatt tgctttctcc taaaaatttg
      601 acgacaaatc ctacatcttt gcttgtccga acagacaaac gttttgcagg agctgtatct
      661 gctgaattaa cacgtcaatt tggcagttat gtcgatgttc ggacatgggg tggacctact
      721 gccatcttag agattgtttc aaaaggaatc caaaaagcaa aaggcgtgca agagattgct
      781 aactacctgt ctatcgatca aaaagacgtc attgcctttg gtgacgagca taatgatttg
      841 gaattgttgg attatgcagg ttggggagta gctatggcta acggtactga ccaactcaaa
      901 ggcattgcaa atgatgtcac tcctttgagc aatcaggaag acggtttagc tgtttatctg
      961 gaaaaactac ttaaactata atagagaatt agattccaca aaaaagaggt tgtga
//
LOCUS       DAYYKC010000240         1014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000240 DAYYKC010000000
VERSION     DAYYKC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.248"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1014
                     /locus_tag="ACHBBW_003079"
     CDS             <1..>1014
                     /locus_tag="ACHBBW_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154678.1"
                     /translation="VKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSLESIF
                     SSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHY
                     VMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSK
                     KESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVT
                     LLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLL
                     ICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFS
                     TLFI"
BASE COUNT          322 a          140 c          138 g          414 t
ORIGIN      
        1 gtaaaaatat ttatagttta tgcatttgtt agtttattat atttatttcc agcatttaaa
       61 aacatggaaa tatttggctt ttatgatacg gcatttcaca ttaatcgtgc gttaagttta
      121 gaatctattt ttagctcccc tattaatttt gaaacgttta gatcatatgg catgcaagta
      181 aataattttt acccatggtt aactctatat cctttatttt tattgatcaa gtttactaat
      241 ttagcaatag gctataacct ttttctttat attgttacgc tcattactct atttatatgt
      301 cattatgtaa tgtatgaaat taccaaaaaa catgttacta gctccttttt cgctattata
      361 tatacaacat cttcttttag gagtgtagaa atatttcttc gaggggcaat gggggaatta
      421 ctcgcaatgt caatacttcc tcttatttta ctcgggttta taaagcttta cgactcaaaa
      481 aaagaatctt gggttatgtt ggcgattagc atgactttat taatttacac tcatgttctt
      541 tctgtagcta tgactatggt aatgattata attgctttaa ttattcaatt ttatcggaaa
      601 aagaaaatag aaatcttctt aattattaaa ttaataaaag ctgctatagt tactttactt
      661 ctttcactag cgtttatagg tcctatgatt gagcagacat tgtatcaaga tctgaataga
      721 ccttatgtgg atatattaca aaaaagagca atttatctgg gtaaagagtt tttgattgga
      781 agtatagatt cagaattact atcatttggt attggcttaa ctttgcttat ttgtttaatt
      841 atactaacat ttaactttaa aaaattagga atcttatcta aaatcactta tataatgggt
      901 ataatatcta ttctactttg tacaaaaata tttccatggt ttgccttaca aaatactcct
      961 ttaaatatta ttcaagctcc ttggcgtttt atgatatttt caacattgtt cata
//
LOCUS       DAYYKC010000241          985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000241 DAYYKC010000000
VERSION     DAYYKC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.249"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>985
                     /locus_tag="ACHBBW_003080"
     CDS             <1..>985
                     /locus_tag="ACHBBW_003080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9154679.1"
                     /translation="EPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITDS
                     EYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLD
                     DTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGK
                     KNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGS
                     IAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYE
                     NFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          262 t
ORIGIN      
        1 tagaaccagg cgattttatc atgagtggcg ctggaacaat tgggagaatt tcaagagtac
       61 caaagcaaat aaaaccgggt gtattcaatc aggctttgat tagacttaga ataaataaag
      121 aaatcacaga ttcagaatat tttattcaat ttattcgtgc agattttatg caaagaaaac
      181 tcacaggagc taatccaggt tcagcaatta caaatctcgt accaatgtca gaagtgaaaa
      241 aatggatagt acaatttcca atattagaag aacaaaagaa aattgggaac ttcttcaaac
      301 aactggacga cactatcgct cttcaacaac gtaagctaga tttattaaag gaaacgaaaa
      361 aaggattctt acaaaaaatg ttccctaaaa acggagcaaa agtgccagaa attcggtttc
      421 ctggatttac ggaagattgg gaacagcgta agttcaaaga gtttagtaag aagactggaa
      481 aaaagaacac gaaagattta gacttccctg cctattcggt aagtaataaa gcaggtttaa
      541 tttctcaaac agaacagttt gatggtagta ggttggatga tttagaaaaa acaaattata
      601 aattcgttga accaaatgaa ttcgcctata atccagcaag agtaaacgta ggatcaattg
      661 catttaacaa tcttggtatg acggtcattg ttagctctct ttatgtagtt gtgaaaatga
      721 gcgaggactt agataatgag ttcatacttc agtttatcaa gtcaccaaca tttatcaaag
      781 aggtcaagcg caacactgaa ggaagcgtac gagaatactt attctatgag aatttcgcta
      841 atatcaagtt tccttttact aggaataaag aggaacaaca aaaaatcggc gctttcttca
      901 aacaactcga cgacacgatc gctcttcatc aacgtaagtt agatttgctg aaggaaacga
      961 aaaaaggctt cttacaaaag atgtt
//
LOCUS       DAYYKC010000242          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000242 DAYYKC010000000
VERSION     DAYYKC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.250"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHBBW_003081"
     rRNA            <1..>943
                     /locus_tag="ACHBBW_003081"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYKC010000243          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000243 DAYYKC010000000
VERSION     DAYYKC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.252"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHBBW_003082"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHBBW_003082"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAYYKC010000244          833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000244 DAYYKC010000000
VERSION     DAYYKC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.253"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHBBW_003083"
     tRNA            complement(20..91)
                     /locus_tag="ACHBBW_003083"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Gln,seq:ttg)
     gene            complement(103..175)
                     /locus_tag="ACHBBW_003084"
     tRNA            complement(103..175)
                     /locus_tag="ACHBBW_003084"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:His,seq:gtg)
     gene            complement(178..251)
                     /locus_tag="ACHBBW_003085"
     tRNA            complement(178..251)
                     /locus_tag="ACHBBW_003085"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Trp,seq:cca)
     gene            complement(258..338)
                     /locus_tag="ACHBBW_003086"
     tRNA            complement(258..338)
                     /locus_tag="ACHBBW_003086"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:Tyr,seq:gta)
     gene            complement(340..415)
                     /locus_tag="ACHBBW_003087"
     tRNA            complement(340..415)
                     /locus_tag="ACHBBW_003087"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Phe,seq:gaa)
     gene            complement(451..523)
                     /locus_tag="ACHBBW_003088"
     tRNA            complement(451..523)
                     /locus_tag="ACHBBW_003088"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Asp,seq:gtc)
     gene            complement(533..605)
                     /locus_tag="ACHBBW_003089"
     tRNA            complement(533..605)
                     /locus_tag="ACHBBW_003089"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Val,seq:tac)
     gene            complement(621..692)
                     /locus_tag="ACHBBW_003090"
     tRNA            complement(621..692)
                     /locus_tag="ACHBBW_003090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Glu,seq:ttc)
     gene            complement(707..796)
                     /locus_tag="ACHBBW_003091"
     tRNA            complement(707..796)
                     /locus_tag="ACHBBW_003091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Ser,seq:gga)
BASE COUNT          235 a          222 c          194 g          182 t
ORIGIN      
        1 aaagaataaa ttatagaaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
       61 gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg atgggaatcg
      121 aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatgg
      181 caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt ctacctttaa
      241 actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta aggaacggat
      301 ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg gacagaatcg
      361 aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc gcgccatatt
      421 ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa cccgcgatct
      481 cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat tatggaggtt
      541 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
      601 cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
      661 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga gagtaaggga
      721 ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc tccttcggcc
      781 tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat gag
//
LOCUS       DAYYKC010000245          812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000245 DAYYKC010000000
VERSION     DAYYKC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.254"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHBBW_003092"
     CDS             complement(<1..613)
                     /locus_tag="ACHBBW_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM9154680.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          245 a          158 c          150 g          259 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gc
//
LOCUS       DAYYKC010000246          800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000246 DAYYKC010000000
VERSION     DAYYKC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.255"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>800
                     /locus_tag="ACHBBW_003093"
     CDS             <1..>800
                     /locus_tag="ACHBBW_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM9154681.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKL"
BASE COUNT          273 a          117 c          148 g          262 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta
//
LOCUS       DAYYKC010000247          754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000247 DAYYKC010000000
VERSION     DAYYKC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.256"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /locus_tag="ACHBBW_003094"
     CDS             <1..216
                     /locus_tag="ACHBBW_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154682.1"
                     /translation="KRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNS
                     ISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(307..672)
                     /locus_tag="ACHBBW_003095"
     CDS             complement(307..672)
                     /locus_tag="ACHBBW_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154683.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          283 a          103 c          129 g          239 t
ORIGIN      
        1 aaacgaagtc tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga
       61 attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc
      121 acctcaaatt caatatctat tattggaatc attattgctg caattattac acaactgttt
      181 ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga
      241 tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta catatatggt
      301 ttgtttttat ggatgccgag cttttttata agataaggct accgcccata acgacccaag
      361 gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg gttttaaagg
      421 atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga
      481 tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat aatatccagc
      541 aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac gaaaatcaag
      601 cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt ttttccagca
      661 agtttttatc atctttcatc accacctaac aagtgttatt attttgttag ttttattgta
      721 aattgaatcc tgcaataatc aattactttt tcat
//
LOCUS       DAYYKC010000248          754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000248 DAYYKC010000000
VERSION     DAYYKC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.257"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..733
                     /locus_tag="ACHBBW_003096"
     CDS             62..733
                     /locus_tag="ACHBBW_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9154684.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          304 a          113 c          117 g          220 t
ORIGIN      
        1 tattatatgg catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg
       61 tatgacaatt aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat
      121 ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc
      181 tattggtcgt acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga
      241 tacaattcct caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc
      301 tcgatttgtc agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa
      361 taaccaaaaa aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca
      421 aaaaaatgct cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat
      481 tgcagagcga gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt
      541 tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta
      601 tcaactattt gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt
      661 gcatcttcat ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt
      721 caaaaatata taacttttaa ctctttttaa atta
//
LOCUS       DAYYKC010000249          746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000249 DAYYKC010000000
VERSION     DAYYKC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.258"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>746
                     /locus_tag="ACHBBW_003097"
     CDS             <1..>746
                     /locus_tag="ACHBBW_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9154685.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          251 a          134 c          135 g          226 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttaa gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa
      541 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct
      601 tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat
      661 ttgaaagcat attactggct tacagtgttc tattaagtcg agcacaacga cgttttgaag
      721 gaactttgag atattcttta ggatat
//
LOCUS       DAYYKC010000250          728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000250 DAYYKC010000000
VERSION     DAYYKC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.260"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>728)
                     /locus_tag="ACHBBW_003098"
     CDS             complement(37..>728)
                     /locus_tag="ACHBBW_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154686.1"
                     /translation="DKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRR
                     ELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFF
                     LFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKP
                     EFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQID
                     DFPEEIIDRIE"
BASE COUNT          226 a          129 c          109 g          264 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcat
//
LOCUS       DAYYKC010000251          725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000251 DAYYKC010000000
VERSION     DAYYKC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.261"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACHBBW_003099"
     CDS             <1..586
                     /locus_tag="ACHBBW_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9154687.1"
                     /translation="IMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKG
                     TNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRN
                     HKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIR
                     EWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          267 a          115 c          138 g          205 t
ORIGIN      
        1 tattatggac tttgcgattc tttctgatgg acaaaaaatt gataataata aattcacgtc
       61 caaaatggaa aagaaactaa agcgtgaaca acgcaaattg tctaggcgtg ctttattggc
      121 taaaaataaa ggtaccaatc tatttgaagc taaaaattat caaaaacaaa aacgcaaagt
      181 agctagattg catgaaaaag taatgaatca acgtactgat tttctgaata agttgagtac
      241 agaaatgatc aaaaatcacg atattatctg tattgaagac ttaaacacaa aaggtatgtt
      301 gcgaaatcat aaattagcta aaagtatttc tgatgtttct tggtcaaagt ttgtgactaa
      361 attacaatac aaggctgact ggtatggacg aaaaatcatc aaagtagata aatggtttcc
      421 gtctagtcag atttgttcag agtgcggaca taaagatggc aagaaatctc ttgaaattcg
      481 agaatggact tgtcctgttt gtcatactca tcatgatcgt gatatcaatg ctagtatcaa
      541 tattctgacc gaaggtctaa gactacattc tatgggattg gcttagatcc aatcatcaag
      601 aaccgtagga actacgggga tagcttggta aataagagac accgctgtta gtaaagaaat
      661 acgctatcaa gtatgctcta ttcccaagaa gctcccactt caagcgttaa gaaccgtcag
      721 gttta
//
LOCUS       DAYYKC010000252          716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000252 DAYYKC010000000
VERSION     DAYYKC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.262"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..362)
                     /locus_tag="ACHBBW_003100"
                     /pseudo
     CDS             complement(249..362)
                     /locus_tag="ACHBBW_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          262 a          107 c          122 g          225 t
ORIGIN      
        1 tcgaaaagag tatacgaaca aacgttcttt ttgtaaaggt ggaatttatt atactaaata
       61 aataggtgaa caagtacttg tgccaaatta tgtgccaaaa aaaatcgaat ttaataaaac
      121 acaaacaaaa agaaatccta ttatgataac atttcttata ataacaaaca caacagaaga
      181 cgtgtaatag ttagtcagga acgtacaaat aacccctgta tcctttgcgg tacagggggt
      241 tattttgtat aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg
      301 ctaagtatat ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccatca
      361 ataaacagtc gaagagaaaa tagggaaaaa ttgattacta aagaaaaact tactaggtcg
      421 acggtgtagt tgattttcat acttcaatag attcgaatta aacgatttcg attggcttat
      481 accagtccta tccaacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt
      541 tacgtaaagc caatgggaat gaactggaga tgacttttta ttatttttag taatagctga
      601 tgggttaata cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt
      661 tagcgaaata aaggatctaa gccaatagct tagatccttt attctatgct ttatat
//
LOCUS       DAYYKC010000253          701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000253 DAYYKC010000000
VERSION     DAYYKC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.263"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHBBW_003101"
     CDS             <1..156
                     /locus_tag="ACHBBW_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9154688.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>701
                     /locus_tag="ACHBBW_003102"
     CDS             175..>701
                     /locus_tag="ACHBBW_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9154689.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELL"
BASE COUNT          310 a           55 c          122 g          214 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag a
//
LOCUS       DAYYKC010000254          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000254 DAYYKC010000000
VERSION     DAYYKC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.264"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHBBW_003103"
     CDS             complement(54..629)
                     /locus_tag="ACHBBW_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9154690.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYKC010000255          633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000255 DAYYKC010000000
VERSION     DAYYKC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.265"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..298)
                     /locus_tag="ACHBBW_003104"
     CDS             complement(<1..298)
                     /locus_tag="ACHBBW_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154691.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRF"
BASE COUNT          196 a          117 c           98 g          222 t
ORIGIN      
        1 aaaatcgaat ttttctccca gttgatagat caaggggcag aaaacctttt attacattgt
       61 gagtgtcacc atcatttaca atgtcattag gaattttgat tttagaattc tgtggagcac
      121 ctattggaag ataataattc attataattt tgtagtcttc atgaggaaat aaagtacttt
      181 taggattgct aagatattct cgtaccgaat ctaagaatgg cttatccatt gaatcttcat
      241 ccccatttaa actaaaaaac attcctacaa ttgcttttgc aactctaagt gaattcattt
      301 tctttattga atgtgttaaa tcagacttac cattctttaa acttgtattg ttataaagga
      361 aatttttatt gtcttctgca aatctaatat attcctcaac atactcacta ccaaaaaaac
      421 tattacattt actacataac gtattggaat ggcaacagtt tttttgacaa attttataag
      481 gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg
      541 tgacgatctt cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat
      601 ccgagctcat tatcaaggat tcgctgcgcc aat
//
LOCUS       DAYYKC010000256          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000256 DAYYKC010000000
VERSION     DAYYKC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.266"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHBBW_003105"
     tRNA            complement(65..150)
                     /locus_tag="ACHBBW_003105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHBBW_003106"
     tRNA            complement(167..238)
                     /locus_tag="ACHBBW_003106"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHBBW_003107"
     tRNA            complement(250..322)
                     /locus_tag="ACHBBW_003107"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHBBW_003108"
     tRNA            complement(340..421)
                     /locus_tag="ACHBBW_003108"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHBBW_003109"
     tRNA            complement(429..501)
                     /locus_tag="ACHBBW_003109"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHBBW_003110"
     tRNA            complement(505..577)
                     /locus_tag="ACHBBW_003110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYKC010000257          578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000257 DAYYKC010000000
VERSION     DAYYKC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.267"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACHBBW_003111"
     CDS             complement(122..499)
                     /locus_tag="ACHBBW_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154692.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          172 a          105 c          102 g          199 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtc
      541 aattctaata taccatcatt aagtataaat gtaaacgc
//
LOCUS       DAYYKC010000258          544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000258 DAYYKC010000000
VERSION     DAYYKC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.268"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>544
                     /locus_tag="ACHBBW_003112"
     CDS             <1..>544
                     /locus_tag="ACHBBW_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM9154693.1"
                     /translation="RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLK
                     NRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKD
                     PDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKR
                     VSYGFKSFENMRIRIFLINQL"
BASE COUNT          192 a          106 c          100 g          146 t
ORIGIN      
        1 tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat
       61 gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg
      121 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      181 tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt
      241 ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga
      301 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg
      361 ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat
      421 ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg
      481 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
      541 atta
//
LOCUS       DAYYKC010000259          539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000259 DAYYKC010000000
VERSION     DAYYKC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.269"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHBBW_003113"
     CDS             complement(<1..450)
                     /locus_tag="ACHBBW_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9154694.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          154 a           99 c           66 g          220 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagt
//
LOCUS       DAYYKC010000260          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000260 DAYYKC010000000
VERSION     DAYYKC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.270"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHBBW_003114"
     CDS             <1..>534
                     /locus_tag="ACHBBW_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9154695.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYKC010000261          522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000261 DAYYKC010000000
VERSION     DAYYKC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.271"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>522)
                     /locus_tag="ACHBBW_003115"
     CDS             complement(<1..>522)
                     /locus_tag="ACHBBW_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9154696.1"
                     /translation="EQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYT"
BASE COUNT          177 a           66 c           85 g          194 t
ORIGIN      
        1 gtatattttt taaaagctga agaaaaatga tggatcgaat gaaatcctaa tatatctgca
       61 atttctgtat aggttaattc attttctagc acgagacgtt ttgctttttc catttttagt
      121 tctttaaaat agctaataac actttttcca ttcacttttc taaaatgttt ttttaagctg
      181 gctggactga taaaaaaaat ttgggctaat tgttcaagca aaaaattttc tgaaagatgt
      241 tcttctaaat atcgtatgat tggatcaact aattcatctg aataaagaat atgctttttt
      301 ttaggaatga ctttctcatt tgaaggtatt gtcaataaag caagttcctg ttgataatgc
      361 cttggtaagg aagacaattg aattttttgc agacaataag taactaataa ggaaagcaaa
      421 tgaatgctca tctgttggca tagtatgaaa aaagaattga tttcctgatc ataggaaata
      481 gctggcttac tatgttttag ataatcaatt ttgaactgtt ca
//
LOCUS       DAYYKC010000262          515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000262 DAYYKC010000000
VERSION     DAYYKC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.272"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>515
                     /locus_tag="ACHBBW_003116"
     CDS             <1..>515
                     /locus_tag="ACHBBW_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9154697.1"
                     /translation="VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLN"
BASE COUNT          196 a           64 c           87 g          168 t
ORIGIN      
        1 agttcattat ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata
       61 tacagaaaat ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa
      121 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
      181 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      241 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      301 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      361 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      421 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      481 aatttcttct gtcgttcaat tcatcggctt aaata
//
LOCUS       DAYYKC010000263          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000263 DAYYKC010000000
VERSION     DAYYKC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.273"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHBBW_003117"
     CDS             complement(<1..132)
                     /locus_tag="ACHBBW_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9154698.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYKC010000264          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000264 DAYYKC010000000
VERSION     DAYYKC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.274"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACHBBW_003118"
     CDS             complement(<1..>487)
                     /locus_tag="ACHBBW_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154699.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAYYKC010000265          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000265 DAYYKC010000000
VERSION     DAYYKC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.275"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>479
                     /locus_tag="ACHBBW_003119"
     CDS             <1..>479
                     /locus_tag="ACHBBW_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9154700.1"
                     /translation="TFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     "
BASE COUNT          193 a           74 c          112 g          100 t
ORIGIN      
        1 accttcaccg caggagaagg aacaagtatt gaaagtggaa caacagtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagagga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatttg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DAYYKC010000266          477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000266 DAYYKC010000000
VERSION     DAYYKC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.276"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHBBW_003120"
     CDS             <1..375
                     /locus_tag="ACHBBW_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154701.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT          210 a           47 c           77 g          143 t
ORIGIN      
        1 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
       61 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      121 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      181 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      241 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      301 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      361 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      421 tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta gtttatt
//
LOCUS       DAYYKC010000267          470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000267 DAYYKC010000000
VERSION     DAYYKC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.277"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..193)
                     /locus_tag="ACHBBW_003121"
     CDS             complement(<1..193)
                     /locus_tag="ACHBBW_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154702.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEAR"
     gene            complement(210..>470)
                     /locus_tag="ACHBBW_003122"
     CDS             complement(210..>470)
                     /locus_tag="ACHBBW_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154703.1"
                     /translation="NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTI
                     SEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          166 a           87 c           47 g          170 t
ORIGIN      
        1 ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca tattcattta
       61 acacactatt attaccatta cttagacgct cagattctac gaaacaactc aaatcagaaa
      121 tttcccaaaa aaattttttg ttaaaatcca atttcctcaa ctcctccaaa ctacgtaaat
      181 gatatagtct cataataaaa taaaacctct cagctaatgt tgttgaaatt gtccactaaa
      241 cctaattgat attgcttagg agatacacct gcgtactttt tgaatactct cgaaaagtgt
      301 gaagaatctg aaaatcctaa acctaaagaa acttcactga tagtatggtt tgatttcaac
      361 attattttag ccgtagaaat ttttcgctta gagatataat tataaatcgt aatatgcatt
      421 tcattcttaa agctttctct taatttagtt tcacttacag aaaaataatt
//
LOCUS       DAYYKC010000268          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000268 DAYYKC010000000
VERSION     DAYYKC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.278"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHBBW_003123"
     CDS             <1..>451
                     /locus_tag="ACHBBW_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154704.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYKC010000269          441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000269 DAYYKC010000000
VERSION     DAYYKC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.280"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>441
                     /locus_tag="ACHBBW_003124"
     CDS             <1..>441
                     /locus_tag="ACHBBW_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9154705.1"
                     /translation="IGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIA
                     NKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTY
                     SIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFE"
BASE COUNT          167 a           40 c           80 g          154 t
ORIGIN      
        1 atagggatct tttctggtaa aaaacaggaa acatatacag gattatcttc tgattttagt
       61 gaaaatatgg tgtttgtaga ttattcaaca agccaagaga tattaaataa ttcagaaaat
      121 aatgaaattg caaataaaat tttaatgtat tctggtagtg cagaatctac agacttagcc
      181 ttaaacaaat tgaaagagct taaaattgat gagtcaaagt attttgttga aaaagattct
      241 aatgcgtttg aagagtcttt agagtcagtg agtggaataa aatatataat aaaagtaatg
      301 acttattcta ttatgttagg tgggatggtt gttctttcat taatcttgat tctatggtta
      361 agggaaagaa tttatgaaat aggtatattc ttatctattg gaacatctaa gatacaaatt
      421 ataatgcaat ttatattcga g
//
LOCUS       DAYYKC010000270          438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000270 DAYYKC010000000
VERSION     DAYYKC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.282"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..438
                     /locus_tag="ACHBBW_003125"
                     /pseudo
     CDS             1..438
                     /locus_tag="ACHBBW_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          183 a           73 c           82 g          100 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtac
//
LOCUS       DAYYKC010000271          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000271 DAYYKC010000000
VERSION     DAYYKC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.283"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHBBW_003126"
     CDS             complement(<1..339)
                     /locus_tag="ACHBBW_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154706.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYKC010000272          413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000272 DAYYKC010000000
VERSION     DAYYKC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.284"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..>413
                     /locus_tag="ACHBBW_003127"
     CDS             131..>413
                     /locus_tag="ACHBBW_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9154707.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDP"
BASE COUNT          145 a           56 c           74 g          138 t
ORIGIN      
        1 tattagcttt ttccgaaaat aaacaacctg taactgcaaa aaaagaaaca gtattctctg
       61 aaggcaaaat aaaaactcga tatatattag aggtagagac taagtttaaa aatagatcgg
      121 agtaatggtt atgcttttta atgagacgca attatggttt aaatttgatc cttcgaatag
      181 atttgtcaaa gatttttata aggtgtggga ttcagaagtt ttctttttag caatcgaaga
      241 tagcttatta attaatctct actattctaa taagaactac tttaaaatcc ctgctgcgaa
      301 aactagaatg aagaaagacg tatatttttt gtttgatatc gtgactgatg tgctagacgc
      361 tcgaagcgat catcggcgtt atgactatat aaagtatact ttcgttgatc cag
//
LOCUS       DAYYKC010000273          410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000273 DAYYKC010000000
VERSION     DAYYKC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.285"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..351
                     /locus_tag="ACHBBW_003128"
                     /pseudo
     CDS             127..351
                     /locus_tag="ACHBBW_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          140 a           59 c           72 g          139 t
ORIGIN      
        1 tagatacaat atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga
       61 gttactttca atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt
      121 gatgaaattg ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat
      181 tggtctaatg gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa
      241 aataaagtta ttccagtatt gctatatgga acacataaca atttttatga attagccgag
      301 aacactcgca actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta
      361 tatcaatcaa ccgaccgaca tatgttaaat ctatatataa taaaaatacc
//
LOCUS       DAYYKC010000274          361 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000274 DAYYKC010000000
VERSION     DAYYKC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.290"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..320
                     /locus_tag="ACHBBW_003129"
                     /pseudo
     CDS             129..320
                     /locus_tag="ACHBBW_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          150 a           44 c           55 g          112 t
ORIGIN      
        1 atatatagaa tcaaaatacg aatcttattt tgatttttct agttttttga aaaaagcacc
       61 ctttgatcgt tgtattagtg gaaaagagaa aaaggagcat acttatcatg tcacaaaact
      121 acaactaaat atctaaacaa caccaatttg atgcctattg aaaaaaagta ttgcgaaatg
      181 aagccaagtc tattagaaaa tgaaataata gaataaaaga aaatgggaaa ccgctgaatg
      241 atgtaaatga aggtaagtat ccacgaacta attttgatga acaagatatc tattttttat
      301 ttgtattgga gatactagtt taatatcaaa agttatcagc agtcataatt agttatccaa
      361 t
//
LOCUS       DAYYKC010000275          336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000275 DAYYKC010000000
VERSION     DAYYKC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.291"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>336
                     /locus_tag="ACHBBW_003130"
     CDS             <1..>336
                     /locus_tag="ACHBBW_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9154708.1"
                     /translation="FEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHV
                     RYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMS"
BASE COUNT          107 a           66 c           59 g          104 t
ORIGIN      
        1 gctttgaaca atacaatcac atgcctttgg ttatgcgctt aaaaaagcag cgctatacct
       61 gtaaaaactg tcgaacccat tggacggctc aaagttattt tgttcaaccc agacattcaa
      121 tcgcgaatca tgtcagatat aaaattgctt ctttactgac tgaaaaagta tctttatctt
      181 ttattgcgaa aaattgtcag gtatctttga ccaccgttat tcgtacgttg aaagagttta
      241 aaagctactt accaaagcaa tctaagagga ttctccccag agtattgatg gttgatgaat
      301 ttcgttcgca cgcttccata gaagataaaa tgagct
//
LOCUS       DAYYKC010000276          330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000276 DAYYKC010000000
VERSION     DAYYKC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.292"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..>330
                     /locus_tag="ACHBBW_003131"
     CDS             41..>330
                     /locus_tag="ACHBBW_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9154709.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHIT"
BASE COUNT          122 a           63 c           34 g          111 t
ORIGIN      
        1 ttataaatag aaagcttttt atataaaaag agaaaggaaa gtggttcttt tgttaacaaa
       61 caaccaatcc tccaatcttg tcaaaaaaac actaacctat tccatagata cccatcttca
      121 tttatctgct gctccctatt ttcgactact aataaatgaa agcgctaatt tatatgctga
      181 atttgcacat aaatctatag aactagataa tccttccctt ttattcattg ataaacagca
      241 agaagtccaa atcttttcta ctcaaaagga aacacaaact tttatcatta tccagtttta
      301 ttttgatgag caattattag cacatattac
//
LOCUS       DAYYKC010000277          326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000277 DAYYKC010000000
VERSION     DAYYKC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.293"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..>326
                     /locus_tag="ACHBBW_003132"
     CDS             64..>326
                     /locus_tag="ACHBBW_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9154710.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQC"
BASE COUNT          118 a           43 c           68 g           97 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgttt
//
LOCUS       DAYYKC010000278          317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000278 DAYYKC010000000
VERSION     DAYYKC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.296"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>317)
                     /locus_tag="ACHBBW_003133"
     CDS             complement(<1..>317)
                     /locus_tag="ACHBBW_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM9154711.1"
                     /translation="NFLIPSIYNFGYLLITLIVIFPADFSQSEEEKKKKMVYFNNVFI
                     IIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSI
                     ILS"
BASE COUNT          133 a           47 c           40 g           97 t
ORIGIN      
        1 aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt gtatagacac
       61 caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt cctgttggga
      121 catgataaga aatcaaaaaa ataaattgat agatagatat tagcgcagct aaaaaaatta
      181 tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct tcactttgtg
      241 aaaagtctgc aggaaaaatt actatcaaag taatcagcaa atatccaaaa ttataaatag
      301 atggaattaa aaaattt
//
LOCUS       DAYYKC010000279          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000279 DAYYKC010000000
VERSION     DAYYKC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.297"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHBBW_003134"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHBBW_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYKC010000280          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000280 DAYYKC010000000
VERSION     DAYYKC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.298"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBBW_003135"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBBW_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9154712.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAYYKC010000281          289 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000281 DAYYKC010000000
VERSION     DAYYKC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.300"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>289
                     /locus_tag="ACHBBW_003136"
     CDS             <1..>289
                     /locus_tag="ACHBBW_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9154713.1"
                     /translation="NRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYASRLDE
                     LGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT           97 a           32 c           54 g          106 t
ORIGIN      
        1 taatagaaaa ataacgaata acaaagaact gatcttgtta ctaataggga tttggggtat
       61 ctttttactc tttaatatgt atatgccgac tatgagagcg gatgacgtag tttatgcaag
      121 tagattagat gaacttggct atttgggtgc ttcgatagaa cattataaga catggagttc
      181 aagaattatt atagaactat tcttaatgtt cttttctaaa cactttatgc tatggaaatt
      241 attaaattct acgattatgt taggaattgt agtattactt tgcaagtat
//
LOCUS       DAYYKC010000282          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000282 DAYYKC010000000
VERSION     DAYYKC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.302"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHBBW_003137"
     CDS             <1..>282
                     /locus_tag="ACHBBW_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9154714.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYKC010000283          267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000283 DAYYKC010000000
VERSION     DAYYKC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.303"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..>267)
                     /locus_tag="ACHBBW_003138"
     CDS             complement(35..>267)
                     /locus_tag="ACHBBW_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154715.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaata
//
LOCUS       DAYYKC010000284          264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000284 DAYYKC010000000
VERSION     DAYYKC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.304"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..194)
                     /locus_tag="ACHBBW_003139"
     CDS             complement(<1..194)
                     /locus_tag="ACHBBW_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154716.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGF"
BASE COUNT           95 a           39 c           38 g           92 t
ORIGIN      
        1 taaaatccta tatccgaaaa aaatagcgac tgattgctag caatatctaa agaatcagct
       61 tgttcagcca acaggttgat tgctaaaaag tggaataatt tctttttttc ataagcagac
      121 agaggataat actgttttaa atcttttaac attttttctg tttttataat tataggtagc
      181 gtatacaatt ccacaaaact tatctctgtt gagttaaaaa gattatataa aaaaagccga
      241 atgtttttag gattccctgt caac
//
LOCUS       DAYYKC010000285          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000285 DAYYKC010000000
VERSION     DAYYKC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.307"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHBBW_003140"
     CDS             <1..>253
                     /locus_tag="ACHBBW_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9154717.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYKC010000286          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000286 DAYYKC010000000
VERSION     DAYYKC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.308"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACHBBW_003141"
     CDS             <1..>247
                     /locus_tag="ACHBBW_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154718.1"
                     /translation="TGVLANLIRNGMPKEGEMFNLDKIITWRENWSKNILGELEVGRV
                     YTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNV"
BASE COUNT           95 a           25 c           53 g           74 t
ORIGIN      
        1 actggtgtat tagctaattt aattagaaat ggcatgccaa aagagggaga aatgtttaat
       61 cttgataaga ttattacatg gcgagaaaat tggtctaaaa acatattagg tgagttagaa
      121 gttggtagag tatatactaa caaagaaatt tcagagaagt ttaaatgttc caaacagggt
      181 ggaatgagaa gatctcatca aactaatact cttgtactgt ttagtgatca aacaggaagt
      241 aatgttt
//
LOCUS       DAYYKC010000287          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000287 DAYYKC010000000
VERSION     DAYYKC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.309"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACHBBW_003142"
     CDS             <1..>247
                     /locus_tag="ACHBBW_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9154719.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAYYKC010000288          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000288 DAYYKC010000000
VERSION     DAYYKC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.312"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>233
                     /locus_tag="ACHBBW_003143"
     CDS             22..>233
                     /locus_tag="ACHBBW_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9154720.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           87 a           44 c           44 g           58 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat cat
//
LOCUS       DAYYKC010000289          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000289 DAYYKC010000000
VERSION     DAYYKC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.313"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAYYKC010000290          227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000290 DAYYKC010000000
VERSION     DAYYKC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.315"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACHBBW_003144"
     rRNA            complement(<1..>227)
                     /locus_tag="ACHBBW_003144"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAYYKC010000291          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000291 DAYYKC010000000
VERSION     DAYYKC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.denovo.316"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHBBW_003145"
     CDS             <1..>224
                     /locus_tag="ACHBBW_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM9154721.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAYYKC010000292        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.073, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000292 DAYYKC010000000
VERSION     DAYYKC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.073"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHBBW_003146"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHBBW_003146"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9154722.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHBBW_003147"
     CDS             complement(2159..3031)
                     /locus_tag="ACHBBW_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9154723.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHBBW_003148"
     CDS             complement(3373..4245)
                     /locus_tag="ACHBBW_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9154724.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHBBW_003149"
     CDS             4373..4942
                     /locus_tag="ACHBBW_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9154725.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHBBW_003150"
     CDS             5009..5746
                     /locus_tag="ACHBBW_003150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9154726.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHBBW_003151"
     CDS             5977..7245
                     /locus_tag="ACHBBW_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9154727.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHBBW_003152"
     CDS             7732..8196
                     /locus_tag="ACHBBW_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9154728.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHBBW_003153"
     CDS             8180..8809
                     /locus_tag="ACHBBW_003153"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9154729.1"
                     /translation="MEQKNDFSTIMTGRRPVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHBBW_003154"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHBBW_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9154730.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHBBW_003155"
     CDS             10688..11134
                     /locus_tag="ACHBBW_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9154731.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACHBBW_003156"
     CDS             complement(11333..11848)
                     /locus_tag="ACHBBW_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9154732.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACHBBW_003157"
     CDS             11965..13167
                     /locus_tag="ACHBBW_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9154733.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2425 c         2708 g         3964 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagacccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAYYKC010000293         8032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.115, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000293 DAYYKC010000000
VERSION     DAYYKC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.115"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..605)
                     /locus_tag="ACHBBW_003158"
     CDS             complement(<1..605)
                     /locus_tag="ACHBBW_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9154734.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVAS"
     gene            complement(810..2003)
                     /locus_tag="ACHBBW_003159"
     CDS             complement(810..2003)
                     /locus_tag="ACHBBW_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9154735.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2082..2288)
                     /locus_tag="ACHBBW_003160"
     CDS             complement(2082..2288)
                     /locus_tag="ACHBBW_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9154736.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2281..3423)
                     /locus_tag="ACHBBW_003161"
     CDS             complement(2281..3423)
                     /locus_tag="ACHBBW_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9154737.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3935..4570
                     /locus_tag="ACHBBW_003162"
     CDS             3935..4570
                     /locus_tag="ACHBBW_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9154738.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4706..4798
                     /locus_tag="ACHBBW_003163"
                     /pseudo
     CDS             4706..4798
                     /locus_tag="ACHBBW_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4912..5547
                     /locus_tag="ACHBBW_003164"
     CDS             4912..5547
                     /locus_tag="ACHBBW_003164"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9154739.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5654..6892
                     /gene="mef(H)"
                     /locus_tag="ACHBBW_003165"
                     /gene_synonym="mefH"
     CDS             5654..6892
                     /gene="mef(H)"
                     /locus_tag="ACHBBW_003165"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9154740.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            7119..7538
                     /locus_tag="ACHBBW_003166"
     CDS             7119..7538
                     /locus_tag="ACHBBW_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9154741.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            7543..7782
                     /locus_tag="ACHBBW_003167"
     CDS             7543..7782
                     /locus_tag="ACHBBW_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9154742.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         2455 a         1401 c         1503 g         2673 t
ORIGIN      
        1 atacttgcta caagacagaa aataacaact ggaatataca ctgtagaagc atggaggaaa
       61 aaatatccaa tgattcctat cgtaattaaa ccgatcaatg aaatccctgc cgccagccca
      121 tccaaaccgt caatcagatt gaccgcattc gtgataccga agatccaaaa aatcgttacg
      181 ggaaaactaa gccagcctag ttggatatat ccaataaaag gaagcgtgaa ggaatcaata
      241 cgtatatctg ctacaaaata aataataaga gaagcaatca agatccctat acttttttgt
      301 ttaggtgtca gctcatattt atcatcgagc actcctgtga ctatgattac taaagaagca
      361 atcaagatcg gtacaatata cgattgaggg ataattttac ggaataagaa taaagtcgat
      421 ataacaaatg aaataaagat aggcagtccg cccgctgaag gcattggaac tttatttatt
      481 cttctttcat ttggtttgtc gaccatacct gtttgtactg aaatgatttt gaatacaggt
      541 gttaatatca gtgataataa aattgtcaga aaaaatctta taaccatact aaaagaaaca
      601 agcatatggg cacctacttt aattaagagt cataattatt ataaattata tatgtctttt
      661 tgactatctt tttatatctt ctcaaatgtc cgcaaagcct tattttatcg gctctacggg
      721 cattttgctt ttgtggtaaa cctcgcatat ctaggtctat cttcttatat tttccctgtc
      781 aatcgtggtt aaaatcgtgg taaatacttc tatgcgatta gacgctgaac ctctgatttt
      841 gcggtatcta tggacgcatg agcgtaccag ttcattgtga tactgatgtt tgaatgcccc
      901 atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagccttgt gcagaatgta
      961 tggcgtagcg tatgcggtgt aatatgtggc aagggattgt ccttgtggtg cttgttgtat
     1021 ttctttacca tacggataaa taaggcgtta taatcaatgg caacttttgg attacctttc
     1081 ggattgataa acacaaaacc gctgtgtccg tctacctcta tcgactttat ctttggtcgg
     1141 ctgttgataa cccgttgaaa tgcctgtatc gtttctttgc ttaatggcac ttgccttatc
     1201 ccgcttttcg tcttaggcgt ttcaatataa tagccctgtt ccttactctt tagtaactgg
     1261 tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag tccgcacagt
     1321 tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataatg cttgtgatac
     1381 acgttgtccg tcttaataaa tgacaataag gcttgctcct gttcctctgt caatgcgact
     1441 ttctctttgg tatcattttc tatgacttca cttagcttga aatcaaaagg gtttttcctc
     1501 acataatcat cttgtatggc tatgtagaat gacgctttta acgagcgttt atggttgtta
     1561 atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcttt agcgtcagag
     1621 ggttttatcg tatcaatgct cctagcacct aacttgtcct cttttaataa ccgcatgagt
     1681 tgttcccgtt gtttctgtgt gcttttcttc acatttgtcc tctgtgcgtt ctgtttggca
     1741 tagagttggc aaagcgtcat tttcttgcct gtgctgtcga taccgtcctc aatatcccgt
     1801 cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt tggtgtcggg
     1861 tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttcccaatgc gtctacatat
     1921 ttatataagt attttccgtc tgttcgttgg ctctctccag tatgcaggat acgacctttg
     1981 ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa gagccttgat
     2041 atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc gtccaattta
     2101 tcaataacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt cataatgagc
     2161 cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc aatgcggaaa
     2221 tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat atcctgtgta
     2281 ttactcataa aacaccccct cactttcgat tacgtctgat atgtccgtac acatctgttc
     2341 cacaagcatt tcctgttttt cagaaaggtg ggtacattca tcaatcattt tcatgtagtt
     2401 tgaacctgtc agcctgtcga tttcctctat catcacgatt gacggcatga cctgtctttc
     2461 cagccattgc agagtgcgtt ccatattgac aggtacaggg ttcatttcaa aaggtaaact
     2521 gtcatgggaa atgaatatct cccacaatgg atagtcagga aattccacaa aatcggtaat
     2581 gagataaaac accagcccat gagggttata cgtcaataac agttcttcaa ctgcctgtac
     2641 cgcctttttg tcacgcagac gaatttcaaa acggttgata atatcggtat gtttcttttt
     2701 cgttgcctgt tccttttgtt tttcatacag gcagaaatat tttgtgcttg cctttgagcc
     2761 gatataaagg gtacttgcta aattacggtt ttttccgctt aattttccac cttgtacatt
     2821 ttcatagttt ttacagcgac attccgcacc accatttctg tatttttcag atagcacagg
     2881 aatatccagc agaccgcaca tatcattgat tgccaagtca aaacgtcgga taacaccgcc
     2941 acagtcaaga cagcggttta agaatgaata ccagtccctg ttctgtgcct gtaagacata
     3001 ttccatgttg cgtgagcctg ccccttttaa ttctaaaaat acgcccatgt gttcatcatc
     3061 agaagccatg accttgattt ccccgtaagc ataatgctcc ttatagccat aatacccata
     3121 gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa cttccaatgc
     3181 gtcagtcgtt ggaaaacgga cagacagata atcaacgagc agataaaaag gcttatcgca
     3241 gatatagtaa ttcagacaac gcaggataat gttctgtgtt tccttgctgg cgttagcttt
     3301 tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg caagttctcc
     3361 ttgtgaaaga ccgctggcaa gacgtttgtt cttcatttca agtgcaaatt ctaaattgtt
     3421 cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaaccacc tttcaaaaag
     3481 tgtgatatgt actatcttct taaaacctct cattactact cacaggtttg ggggtgtatg
     3541 gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg gcacagacaa
     3601 ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg tgtcatggcg
     3661 gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag ttctgttttg
     3721 ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa tcagataggt
     3781 atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta caagaacata
     3841 gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc atattaagca
     3901 tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa tgaaaataag
     3961 ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac aacaggaact
     4021 tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag ggttgaacta
     4081 tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc ccaatgaatt
     4141 gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc agtttttaag
     4201 agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact tctttgacag
     4261 cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta cagactatat
     4321 caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta tccagcattt
     4381 gaagtttcaa aaattattag accgctatga tgatttatac agtatggata tgtttgggga
     4441 aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga taaacccgaa
     4501 ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt ttgatgatga
     4561 ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc cgatcaagag
     4621 tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt ctgttgtggg
     4681 caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa aggcaacgca
     4741 ggtatcacag tatgaattga caccgcataa ggaaagcaac tggaagattg tagaataaat
     4801 gaaaaaggct tgcattctgc atttttataa aattgcaagt ctttatattg acaagcataa
     4861 aatactaaga tatactttaa tcagaccaac ggtttgtatt ggaggtaagt tatggcacga
     4921 aataaatatc cagaagaaac agtaaaactc attttagatg tttctacaaa aatgttttgt
     4981 gaaaaaggat atgataacac atcattgcaa gatattatca atgaaacaaa actttctaaa
     5041 ggtgctattt atcatcattt cgattcaaag gaagatattt taaaagcaat ttttcaaaga
     5101 attggaaatg aaaatgcagt aatatttgct aaaattagag ataacgatag tttaaacggt
     5161 ttagaaaagt taagagaaat atttaagaca gcggtattca attctaacca aagcgtttta
     5221 ttgacagttt ctcctcaact attaaacaat cctcgttttc ttgctatgca aatagaacaa
     5281 atgtatgaaa ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg tatcaaggat
     5341 aagtctttgg agattgaaaa ttcacatgaa attgcagaag ttatcatggt attaagtaat
     5401 gtatggctca acccattagt tgaaatgacg gataaagagg gtatggaaaa acgttgcaat
     5461 acattcaatg cccttttaaa tggaataggc atagacacat tattagacga agaaacaatt
     5521 tctgcattta tacagttttg taaataactg tatttgttat ccctatacga tagactgctc
     5581 tataaaagag cagtttattt aaaccattat ataaaccgac ggtctgtttg attaaataaa
     5641 aaggagagat tttatgcaag cagaaaaaaa actttttaat cgtgatttta tacttgttgt
     5701 tataggacag ataatttctt tatttggaaa cgctatccta cgatttgcat taccgtacta
     5761 tttgttaaaa gaaactggtt catcagcttt gtttggtatt gctacagcct gctctttttt
     5821 acctatggtt attttgtcgc tggtaggtgg tattattgct gacaggacaa ataaaagaaa
     5881 cattatggtc atactagatt ttgtaacagc aatcataatt ttagtatttt atttgtcatt
     5941 gggaaaaatt cctatcatac ctttgtttat tgttgctttg atgttacttt atggtatttc
     6001 tggtgcatat caaccagcgg tacaagcaag tattcctcta ttggtatcat cagataaact
     6061 taccgtcggt aatgctataa taaatcaagt aaatacttta gcaaacttta tcggaccagc
     6121 tataagcgga gtagtttttc aattttatgg tatcaatccc attttaattg ttagtattat
     6181 atgttttact ttttcagcca ttatggaaat ttttatccat attccacacc aaaaaagacc
     6241 tcgtgaagta ggtgtaattg cagttgctaa aaacgattta aaagatagtt ataccttttt
     6301 gaaaaaggag aaacccattt ttatatccgt aatctttttg gtttgcattt tcaatttggt
     6361 tttaagtgct gttttaattg ttggtctgcc tatcttaatt tctgaagttt taaaaatgcc
     6421 agatacacta tatggttttt cacaatcagc attatcatta ggtggtttat gtggtggtat
     6481 tttaacagct cttgttgctt caaagttaaa actaaaaaac ctttatacct tgttacttat
     6541 ttgttctgtt tctgttttac ttatgggaat ttcgctattt ttagaattgc ctgcaatagt
     6601 ttcctactgg gtagttacag tgatgtgctt tgtggctatg ggtgtatcta ctatgtttgt
     6661 tgttcaaatt tacactcttg ttcagacaca aacaccaccg caattagtag gaaagataat
     6721 ggctacgctg gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg tttacggtat
     6781 tttattcgat gtatttatac aagaaacatg ggttgttatg tttgtagctt ctattgcttc
     6841 aatcatgatt tcaatatatt caaaaagaat ttttcaaaag ttagagatat aatacaaggg
     6901 gttaaatagt atgttaggct aaaaacaata tataaaagaa aatttgaaat ctgctaaaag
     6961 gatagtgtta taaaagaaag agtaatattg cagtatttaa ggctcgcaca aatatatgtt
     7021 ttgtgtgggc ttttttctta ccatttttct gtatttctaa agggtataaa cggtattccg
     7081 tttatatatt tcagcattag cagaaagggg gtgagattat gaggacatct tctttcgagc
     7141 atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac aaagtgaaaa
     7201 gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt gaaatagccg
     7261 aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt gtatcattta
     7321 aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt ttacatagac
     7381 tgtcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg aacgacagag
     7441 aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga actagaggac
     7501 taaggaagtt aaaaatgctt ttgagcgaaa ggaagtaaag aaatgagtaa agattatggc
     7561 tatccctctt ttgaattgat ttgcaaagcg tcaagcggag atgaaatggc agttaaagag
     7621 attttgaagt tttatgacgg ttatatttct aaattatgct tgcgtccttt ttaccactct
     7681 gaaagtggta aaatcattat gcaggttgat gaagaactaa aaggagaaat tcatacagac
     7741 atgatgaaag caatattgaa atttgaaatc ggagtgaagt aatcaactgt ataaaaaaat
     7801 cgggagatac acagtttcgt gctatctccc ttagtagttt tacggtcata tcaaggctca
     7861 ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct atacttcaaa atgcttgaat
     7921 gtatagtgtt tatgcggata attgaaaatt agatataaaa tttcaaataa attactgata
     7981 taaatttaca ataagaactc ttgtttctac ttttttttat gagaaaattt ag
//
LOCUS       DAYYKC010000294         5906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.138, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000294 DAYYKC010000000
VERSION     DAYYKC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.138"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1107
                     /locus_tag="ACHBBW_003168"
     CDS             211..1107
                     /locus_tag="ACHBBW_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9154743.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1257..2198)
                     /locus_tag="ACHBBW_003169"
     CDS             complement(1257..2198)
                     /locus_tag="ACHBBW_003169"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9154744.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2324..3445
                     /locus_tag="ACHBBW_003170"
     CDS             2324..3445
                     /locus_tag="ACHBBW_003170"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9154745.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3703..5205
                     /gene="eat(A)"
                     /locus_tag="ACHBBW_003171"
     CDS             3703..5205
                     /gene="eat(A)"
                     /locus_tag="ACHBBW_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9154746.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5272..5799
                     /locus_tag="ACHBBW_003172"
     CDS             5272..5799
                     /locus_tag="ACHBBW_003172"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9154747.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1940 a         1041 c         1236 g         1689 t
ORIGIN      
        1 atttaacaat gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt
       61 aggcgaattt taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt
      121 tcatacaaat ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga
      181 aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca
      241 aaaaataaat cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa
      301 gatttactac agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg
      361 atcgtttttt tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc
      421 aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg
      481 aagcaggcac aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca
      541 aaagaacttg ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct
      601 agcgaaccaa agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca
      661 gaaagctata agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca
      721 catcgaggga atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg
      781 gaatcgcgcg ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca
      841 tttgaatcca tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat
      901 catttttatc gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg
      961 gaatttctgc atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca
     1021 gaaaattggg caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat
     1081 gacctctcct ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa
     1141 acaaacaagc acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt
     1201 gggcttaaaa aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat
     1261 tctgcgccaa ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt
     1321 gtagcaatat catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca
     1381 gtcaaacgat ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg
     1441 tcaggatcat aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct
     1501 tcttttgctg cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct
     1561 ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt
     1621 ccatttacac aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt
     1681 aaatttgctt cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc
     1741 aagttctcta gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga
     1801 ccagctgtcc acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt
     1861 acatcttcat gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg
     1921 gattggatcg aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag
     1981 tccacgtgtt cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc
     2041 acttctttac cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca
     2101 ggccaacgat cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg
     2161 ctgcccattg ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa
     2221 tgagttccct tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa
     2281 atgaaaaaaa gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc
     2341 aattgattca tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg
     2401 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
     2461 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
     2521 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
     2581 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
     2641 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
     2701 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
     2761 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
     2821 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
     2881 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
     2941 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
     3001 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
     3061 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
     3121 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
     3181 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
     3241 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
     3301 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
     3361 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     3421 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     3481 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     3541 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     3601 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     3661 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     3721 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     3781 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     3841 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     3901 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     3961 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     4021 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     4081 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     4141 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     4201 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     4261 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     4321 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     4381 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     4441 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     4501 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     4561 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     4621 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     4681 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     4741 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     4801 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     4861 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     4921 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     4981 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     5041 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     5101 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     5161 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     5221 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     5281 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     5341 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     5401 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     5461 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     5521 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     5581 gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt
     5641 cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat
     5701 gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca
     5761 ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa
     5821 ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggct ctttgtcaat
     5881 aaggactgat gggttgtaca aaatca
//
LOCUS       DAYYKC010000295         5827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000295 DAYYKC010000000
VERSION     DAYYKC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.141"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1040)
                     /locus_tag="ACHBBW_003173"
     CDS             complement(<1..1040)
                     /locus_tag="ACHBBW_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9154748.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEA"
     gene            1319..2125
                     /locus_tag="ACHBBW_003174"
     CDS             1319..2125
                     /locus_tag="ACHBBW_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9154749.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2143..2850
                     /locus_tag="ACHBBW_003175"
     CDS             2143..2850
                     /locus_tag="ACHBBW_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9154750.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            2847..3818
                     /locus_tag="ACHBBW_003176"
     CDS             2847..3818
                     /locus_tag="ACHBBW_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9154751.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3811..4839
                     /gene="vanB"
                     /locus_tag="ACHBBW_003177"
     CDS             3811..4839
                     /gene="vanB"
                     /locus_tag="ACHBBW_003177"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9154752.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            4845..5453
                     /locus_tag="ACHBBW_003178"
     CDS             4845..5453
                     /locus_tag="ACHBBW_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9154753.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5519..5692)
                     /locus_tag="ACHBBW_003179"
     CDS             complement(5519..5692)
                     /locus_tag="ACHBBW_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154754.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1650 a         1206 c         1471 g         1500 t
ORIGIN      
        1 gcctctttgt cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat
       61 tcgtcagaga tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca
      121 aaattgcgcc atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc
      181 cgagacaacg cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag
      241 cttttcatga ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga
      301 aaacgatcca caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa
      361 acattcatgt ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata
      421 aaataagaaa cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta
      481 tcgccatcca tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca
      541 tccattaata aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa
      601 gcgagtgtcc gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt
      661 gcttgttttt cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag
      721 tttctatcga caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag
      781 cgttgtttcg ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc
      841 gattttttct ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga
      901 cagcgacgaa tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg
      961 ctcttcgatt ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg
     1021 atatgatgaa gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct
     1081 aacggagctt ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc
     1141 tatcagtcct agaaattata gagccgattt tgaccgacag gtataaccct gccggtcttt
     1201 ttttgctgct acaggaaaac tacagactgg ctacagggaa agtacagatg cgcttgccat
     1261 aataacaatc gtaccagcca caaatcgtag ttttattgca aaggaggcat tcgatcaaat
     1321 ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt
     1381 ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt
     1441 ggggtggaga ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca
     1501 tacaggcagc agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa
     1561 agaacaggga gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc
     1621 cgcacagtac gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat
     1681 ttctccctat cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt
     1741 cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga
     1801 atataaggcg aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc
     1861 cgtgccggga acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat
     1921 tcattcaaca ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt
     1981 tattcgtcgc tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca
     2041 ttaccgatat gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga
     2101 ggaatattta aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg
     2161 acacagcgtt tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat
     2221 gcgggaatga gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc
     2281 catttgctct ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata
     2341 taccaggaaa acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg
     2401 ataagaccgg gggaaacctt ttctttctgg ttgttcctgc atacgccatt gacaattatc
     2461 cagcgcagag gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg
     2521 gtggatgcaa ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc
     2581 acctaccaaa tatgggtgac cttagatgat gagaaaatca tcggtcaggt gttcgccgac
     2641 aaagagcccc aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt
     2701 ggcgggattt atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa
     2761 ataatagatt gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa
     2821 agtgtggata ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat
     2881 gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta
     2941 cgctgattag cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg
     3001 ttagcgtaag ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg
     3061 gggtaaaata catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg
     3121 agagaatggg aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg
     3181 ctttgatgct gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc
     3241 aacaagattt cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag
     3301 ttattggaac cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc
     3361 atgtgctggc ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc
     3421 ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc
     3481 tgatcggtca gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc
     3541 gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg
     3601 gtgcggcact ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga
     3661 aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac
     3721 cccatacggc gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga
     3781 attgtctcaa ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc
     3841 ggcggttgct cggaggaaca tgatgtgtcg gtaaaagccg caatagaaat tgctgcgaac
     3901 attgatacgg aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag
     3961 ctatgcaaga agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg
     4021 gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt
     4081 gatgtggctt tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg
     4141 tttgtattgt ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg
     4201 gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa
     4261 atgattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag
     4321 ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac
     4381 gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg
     4441 ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg
     4501 gatcaaatcc ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa
     4561 ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg
     4621 gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt
     4681 gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac cctgcccggt
     4741 tttacatcgt acagccgcta cccacgtatg atggccgccg caggaatcac gcttcctgca
     4801 ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt
     4861 tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata
     4921 acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg
     4981 tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt
     5041 tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac
     5101 cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta
     5161 caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc
     5221 tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg
     5281 aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct
     5341 tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata
     5401 agttgattga tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt
     5461 attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt
     5521 actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc
     5581 cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt
     5641 ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg
     5701 gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc
     5761 accactcaaa aaaagccttg cccctccatg aactgaaccc caaaagttga actatttaat
     5821 ggactgt
//
LOCUS       DAYYKC010000296         3796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000296 DAYYKC010000000
VERSION     DAYYKC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.168"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHBBW_003180"
     CDS             110..193
                     /locus_tag="ACHBBW_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9154755.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBBW_003181"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBBW_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9154756.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHBBW_003182"
     CDS             complement(1318..2181)
                     /locus_tag="ACHBBW_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9154757.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHBBW_003183"
     CDS             complement(2183..2455)
                     /locus_tag="ACHBBW_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9154758.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHBBW_003184"
     CDS             complement(2472..2681)
                     /locus_tag="ACHBBW_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9154759.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHBBW_003185"
     CDS             complement(2759..3655)
                     /locus_tag="ACHBBW_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9154760.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAYYKC010000297         3571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.173, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000297 DAYYKC010000000
VERSION     DAYYKC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.173"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..213
                     /locus_tag="ACHBBW_003186"
     CDS             28..213
                     /locus_tag="ACHBBW_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9154761.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            371..538
                     /locus_tag="ACHBBW_003187"
     CDS             371..538
                     /locus_tag="ACHBBW_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154762.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            770..943
                     /locus_tag="ACHBBW_003188"
     CDS             770..943
                     /locus_tag="ACHBBW_003188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154763.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            1156..1815
                     /gene="vanR-B"
                     /locus_tag="ACHBBW_003189"
     CDS             1156..1815
                     /gene="vanR-B"
                     /locus_tag="ACHBBW_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9154764.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1818..3179
                     /gene="vanS-B"
                     /locus_tag="ACHBBW_003190"
     CDS             1818..3179
                     /gene="vanS-B"
                     /locus_tag="ACHBBW_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9154765.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(3270..>3571)
                     /locus_tag="ACHBBW_003191"
     CDS             complement(3270..>3571)
                     /locus_tag="ACHBBW_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9154766.1"
                     /translation="YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESG
                     IRLALELPYSNAKIENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1060 a          675 c          894 g          942 t
ORIGIN      
        1 ttataatgta ccctgtggag gtgtactatg gactacatga cgttgaaaga ggcaggcgag
       61 aaatggggtg tgcccccacg atggataaac tactattgtg ctgcgggacg catccccggc
      121 gctgtaaaga tggcgacgat ctggctcatt cccaaaactg cggaaaagcc gattgacagg
      181 cgtacaaaga aaggaaaggc ggttactgaa tagtataatg aaaccggcgc attttggata
      241 tggttttgaa gagagagaaa gcatggtatt ttgtcacgga aaaactataa gatgatagat
      301 ggcaggctgc tccagaccga taaaaagtat tctcatctga aacagaaaga aaagatttac
      361 acatggatgt atgaagaagc gaaactttat catgacagaa atggaaaatg cccgaaacaa
      421 agacgaggaa gctgctgctg tatcagccgt atatggtaga attgaaaagg ccggcatctg
      481 gattctttat ggtgaagtct ccagacattg gataactgct ggtctaatgc gcctttgagt
      541 atggggtgat tgattttaat accggggagg tacggctttt tgaggaaaat attctgggat
      601 ttacatttgg cgattattac atttcctgta actgcaagga cacggatgat gaagatatga
      661 ggaatataga cttgctgatt ttctactgtc ttgaacggta ttaggactga atctgctgcg
      721 cggagtgcgg caagagcgac cggagggagt acacacaggc cctgcctgtg tgaaaagcca
      781 taccgcaagg tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc
      841 caacaagcaa aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg
      901 atattctgat ccattgcttg ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa
      961 acatcccaaa cattaagaag gtgttaatta ttattatctt gatttaattc gtttaaacca
     1021 ataatataga tacatacacc atgaaaagaa gcgagtatcc caatgacgga tgattaaaat
     1081 ccgaaaagaa atcgctttga aactacaggg aaactacaga ctgttatgtt atcttcttaa
     1141 aatggaggga tttttatgtc gatacgaatt ctacttgtcg aggatgatga tcatatctgt
     1201 aatacagtaa gggcgtttct ggctgaggca ggatatcagg tggatgcctg cacagatgga
     1261 aatgaggcat acaccaagtt ttacgaaaac acttatcaac tggttattct tgatattatg
     1321 ctgcccggta tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc
     1381 attctgatga tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag
     1441 gcagacgact atgtaacaaa gccattcaag atgcagattt tactaaagcg ggtggaagcc
     1501 ctgctgcggc gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct gacacttctg
     1561 ccggaggatt tcacggtact ttgtgacggt acagagctgc ccctgacacg aaaagaattt
     1621 gaaatccttt tgctgctggt gcagaacaaa ggcagaacct taacgcatga aatcattttg
     1681 tcacgtatat ggggatatga ctttgagggt gatggcagca cagtccacac tcatatcaaa
     1741 aatttgcggg ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt aggataccga
     1801 ttggaggaat cataataatg gaaagaaaag ggattttcat taaggttttt tcctatacga
     1861 tcattgtcct gctactgctt gtcggtgtaa cagcaacact gtttgcacag caatttgtgt
     1921 cttatttcag agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac
     1981 tgattcagaa tagcgatagg cttgatattc aagaggtggc agggctgttt cactacaata
     2041 accaatcctt tgagttttat attgaagata aagagggaag cgtactctat gccacaccaa
     2101 atgccaatac atcaaatagt tttagaccca actttcttta tgtggtacat agagatgata
     2161 atatttcgat tgttgctcaa agcaaggcag gtgtgggatt gctttatcaa gggctgacaa
     2221 ttcggggaat tgttatgatt gcgataatgg ttgtattcag ccttttatgc gcgtatatct
     2281 ttgcgcggca aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa
     2341 acctgaaaga tgtaccgccg ccgctggagc gaaaggatga gcttggcgca ctggctcatg
     2401 atatgcattc catgtatgtc aggctgaaag aaaccatcgc aaggctggag gatgaaatcg
     2461 caagggaaca tgagttggag gaaacacagc gatatttctt tgcggcagct tctcatgagc
     2521 taaaaacgcc catcgcggct acaagcgttc tgttggaggg aatgcttgaa aatatcggtg
     2581 actacaaaga tcattctaag tatctgcgcg aatgcatcaa aatgatggat aggcagggca
     2641 aaatcatttc cgaaatactg gagcttgtca gcctgaatga tgggagaatc gtacccatag
     2701 ctgaaccgtt ggacataggg cgcacggttg ccgagttgct gcccgatttt caaaccttgg
     2761 cagaggcaaa caaccagcgg ttcgtcacag atattccagc cgggcaaatt gtcctgtccg
     2821 atccgaggct gctccaaaag gcactatcca atgtcatatt gaatgcagtt cagaacacgc
     2881 cgcagggagg cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc cgcctttttg
     2941 ttttgaacat gggcgttcac attgatgata ctgcgcttcc aaggctgttc accccattct
     3001 atcgcattga tcaggcgcga agcagaaaaa gtgggcgaag cagaaaaagt gggcgaagcg
     3061 gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag tatgcgctgg
     3121 aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca acattgtaaa
     3181 tatttaaaac ttaaatgatt ttgggctctt tgtcaaatag gactgataga gtgagataag
     3241 aacatctaag gtagaattcc ttttctactt tagatgtttt ttgattcata ggtaatcaga
     3301 ttatttagta agaaaattct ggtcttcatt ttccgaaagc ttcgaaagcc ataagcgact
     3361 cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tggcatttga ataaggcaat
     3421 tcgagggcta aacgaatgcc ggattcatag tttataaaag ctttttttat agaatgcagt
     3481 tcttttggaa cggaattggg catcgttctt acaagtttaa agaaagtgtc cgcctctttg
     3541 tcgtgaaacg cttgaagaat ttcttgatag t
//
LOCUS       DAYYKC010000298         2613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000298 DAYYKC010000000
VERSION     DAYYKC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.184"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..388
                     /locus_tag="ACHBBW_003192"
                     /pseudo
     CDS             39..388
                     /locus_tag="ACHBBW_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(660..950)
                     /locus_tag="ACHBBW_003193"
     CDS             complement(660..950)
                     /locus_tag="ACHBBW_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154767.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1352..1588
                     /locus_tag="ACHBBW_003194"
     CDS             1352..1588
                     /locus_tag="ACHBBW_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9154768.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1595..>2613
                     /locus_tag="ACHBBW_003195"
     CDS             1595..>2613
                     /locus_tag="ACHBBW_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9154769.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNE"
BASE COUNT          896 a          438 c          479 g          800 t
ORIGIN      
        1 cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg
       61 caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt
      121 agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata
      181 caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca
      241 aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat
      301 ttacttttac gactatgata atcacctctt cgagttacat accggtactc tccaggaacg
      361 tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct
      421 ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag
      481 caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg
      541 tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg
      601 tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc
      661 tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct
      721 accacaattt tgggtttccc taacacatga taggatctca caacaaattg ctttctatta
      781 ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa
      841 ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca
      901 ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc
      961 tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt
     1021 tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga
     1081 catgtactaa aaaaaagagg cagcagtata gatttaagta gttcagtttt ggagtacaaa
     1141 aagtgaaaat gtaaacctaa atgagtatca aattgcaact gaatttgata ctcgtttttt
     1201 attgttaaat caacgtttgt tgttaattta caccacaaaa tgtaagttaa aatgtatata
     1261 aaaatgcgaa cgtgaaataa ttattgtagg aatgcaaatt ggtgtgttaa aatggttaca
     1321 atttactaag caaaaagggg tgcattttcc tatgcaattt cttgagccaa aaaataagaa
     1381 cgctaagtct gtggattggg aaatatcaga gcaagtaagg gtgatagtta agcaatacgc
     1441 tgaatatgca gagcgtactg aaagcgaagc agtggatgaa ttcttattga acattttaga
     1501 cgataagaag tttattgaat ggattgccaa caagcgaagt aataagcgga ttgttgaaaa
     1561 aatgggtatc aaagatagag tgggttgata gacaatgacc aagcttaaaa gattagccac
     1621 gaaggaagat gaaattgtag atgttaaaat tccgatttca aacgaagaat taaaagatag
     1681 ggctaaacaa tatgatttat taactccaaa acgatttgca acaaggtata acaaaatgtt
     1741 gttcttgcca acgtctttta aatggaacgg ttctgaatat cccattcaat ataattactg
     1801 catcaatccg ttttgttgta attttgggaa agaacagcac aaattcaaag atgtaaaagg
     1861 gaaaccaagt cgatataaaa tgactggtag ttccaaagat aaaggtcata aaggtatgta
     1921 ctgcaatgac aatcctattg gcagaggtgt atcgcaaaac tgtacagtaa cccctttatc
     1981 taattggtct gttgttgaag aaattaaacg ccttatagaa atcaatagca tccaagatgt
     2041 tgagccagat tatcaatttc ataaagaagg ctgctcggaa gaagaatcaa ccccattcaa
     2101 tgagccgaag caattttaca aaagggggaa aagtaggggc aaatctcaac gctatcaatg
     2161 taaggcgtgt aagaagttca ctaatgttct accaaaaaga gaagaaacca ctacttatca
     2221 ccaacaaaag aacaccattc ttccaatgtt cgctaagatg gttgttggaa gggtttcagt
     2281 cagtcgtact tgtgatattt taggaattgg agttggaacc tactatcata agctggagtg
     2341 gctgtatagg cgttgtttgg agtttttaga acgatatgag acacaacccc ttcaaacaaa
     2401 gaaatttaac gagatgtggc ttaatacgga caagatgcac tactacctaa ataacgtccg
     2461 taagaaagga caaggttcaa agaaatatac ggggtttgaa gaccttaata tgcaaacgta
     2521 tatagttgta tctgctgagg tcttatcaag atatgtgttc cgttctgatg ttgcctatga
     2581 ttggaatatt tcaatggatg aattaaatga aga
//
LOCUS       DAYYKC010000299         2346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000299 DAYYKC010000000
VERSION     DAYYKC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.192"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1181)
                     /locus_tag="ACHBBW_003196"
     CDS             complement(78..1181)
                     /locus_tag="ACHBBW_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9154770.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1733..2281
                     /gene="aac(6')"
                     /locus_tag="ACHBBW_003197"
     CDS             1733..2281
                     /gene="aac(6')"
                     /locus_tag="ACHBBW_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9154771.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          744 a          416 c          457 g          729 t
ORIGIN      
        1 caattctgat tcatcagtcc cataaattat agagccaatt tgatgacagc ctgcttgatg
       61 ggggttttag caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca
      121 tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc
      181 acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg
      241 acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag
      301 attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg
      361 acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat
      421 gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta
      481 acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct
      541 ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt
      601 cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct
      661 ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt
      721 gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg
      781 ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg
      841 ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca
      901 cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac
      961 tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt
     1021 tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca
     1081 atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct
     1141 gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt
     1201 cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta
     1261 gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc
     1321 aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt
     1381 gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa
     1441 aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta
     1501 aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct
     1561 ttcctgatct ttcctatttt gaataaagtg tttctgtata attttttatt ttataagtaa
     1621 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
     1681 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
     1741 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
     1801 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
     1861 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
     1921 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
     1981 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
     2041 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
     2101 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
     2161 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
     2221 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
     2281 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
     2341 ttactt
//
LOCUS       DAYYKC010000300          730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6988
            SAMEA115708717-rid22596613.guided.259, whole genome shotgun
            sequence.
ACCESSION   DAYYKC010000300 DAYYKC010000000
VERSION     DAYYKC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708717
            Sequence Read Archive: ERR13198438
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290326.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290326.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:01:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,198
            CDSs (total)                      :: 3,136
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 35 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 27 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708717-rid22596613.guided.259"
                     /isolate="VRE6988"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="ACHBBW_003198"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="ACHBBW_003198"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9154772.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          279 a           78 c          149 g          224 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      721 aatcaaattg
//
